LOCUS       NZ_FPAA01000002        10852 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Marininema halotolerans strain DSM 45789, whole genome shotgun
            sequence.
ACCESSION   NZ_FPAA01000002
VERSION     NZ_FPAA01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Marininema halotolerans
  ORGANISM  Marininema halotolerans
            Bacteria; Bacillota; Bacilli; Bacillales; Thermoactinomycetaceae;
            Marininema.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900116235.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:20:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,453
            CDSs (total)                      :: 3,387
            Genes (coding)                    :: 3,359
            CDSs (with protein)               :: 3,359
            Genes (RNA)                       :: 66
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 5 of 28
            Pseudo Genes (incomplete)         :: 25 of 28
            Pseudo Genes (internal stop)      :: 3 of 28
            Pseudo Genes (multiple problems)  :: 4 of 28
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:12:26
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 308219
            Orig. end                         :: 319071
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FPAA01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10852
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10852
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10852
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5852](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5852)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(352..365)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 20.8"
                     /tool="antismash"
     gene            complement(1028..1993)
                     /locus_tag="CHX35_RS03710"
                     /old_locus_tag="SAMN05444972_102229"
     CDS             complement(1028..1993)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CHX35_RS03710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05444972_102229"
                     /product="hypothetical protein"
                     /protein_id="WP_091834107.1"
                     /transl_table=11
                     /translation="MLIYKQSTFVPIYISGVTRIAVIIPEYEIIHEGLSPLEIARKVYL
                     ESSLINKPTNKLIFIHSIKDKNHYIVKFTQYVPEFDLIDESGDCGNALVASYLFLRDRG
                     DIDKLAKFTSLNTGKVVEITYLGRYSNQHRVQISFKEPVRTINENLLSTNKPILQIKDG
                     DMDYKVTAVNAGNPYIWLRAGDLSPLDLFEWSHREYSLLMRIRSIVQRELGINCHSVFP
                     KVAVVDKPVCKFNHIRTRMITVPSWHGRFALTGVTNLIAGLYTNGTLMTQVGKTMTPSL
                     PYFIEIPGGTIQATGHVKGGKLESVSILQEGYKIGGVNVP"
     gene            complement(1996..3792)
                     /gene="asnB"
                     /locus_tag="CHX35_RS03715"
                     /old_locus_tag="SAMN05444972_102230"
     CDS             complement(1996..3792)
                     /EC_number="6.3.5.4"
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="asnB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /locus_tag="CHX35_RS03715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_102230"
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="WP_091834110.1"
                     /sec_met_domain="GATase_7 (E-value: 1.1e-39, bitscore:
                     129.5, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 6.9e-64, bitscore:
                     211.1, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRVFFLRGVFKICGIVGFHIKGDRYQSYVKLKKCTGSLVHRGPD
                     SEGIYLDDFMALGMRRLSIRDLAFGEQPYYNERKNVVVVFNGEIYNNDRLRCWLESRGH
                     TIDSQSDGAILPHLYEEKGERLFEELDGMFALAIWDAEKKILLLSVDSAGIKPLYYTIQ
                     SDNEFVFASEIKTIHQFDKRPFELDPSAIGSYLLFKSIPTPYTPYKKIRKLKPGEVLIY
                     KTQDQKLVRKYFQPHVSTITDPFKEFFYEQVRTTMLSDVKVGCIASGGLDSNLVLKAMA
                     EQMDEPVPVFTVGYPIETEDDERIYAQQMTKEVNGHFHSVLLNPLEVPEMLEEVIWHLD
                     EPNQDPITLPYYALMREVKEHCKVVLTGDGSDEFFGGYSRFHHWDKNVGLDVTQSQYIS
                     DLLLFSDTQLKEILILQNNAWEPYAEPIQQLNQVRSLKGAMEWEQKFRLPSYHLNRVDK
                     LSMAWGVEARVPFLRPSMIAQARLLSTGDLRIDGVEKVWLKDQASQFMPRWLIERKKKP
                     FTFPMQGWMRDQWKNWIYETLFSSNSIICNFVHKNSLLSIWNMHQKGADYSHHLWSFIV
                     LETFLQLQKGSFNSLYQDQRAG"
     gene            complement(4204..5001)
                     /locus_tag="CHX35_RS03720"
                     /old_locus_tag="SAMN05444972_102231"
     CDS             complement(4204..5001)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CHX35_RS03720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05444972_102231"
                     /product="hypothetical protein"
                     /protein_id="WP_091834114.1"
                     /transl_table=11
                     /translation="MGVLQLQTFLTRYIRDKSLREKVKSLPIETRYSEFGFSKEELKLA
                     VSLDFTQLDRTVNQLDRERRAKRRADFAEFTTVLQQVGVFDSFFSSFCKRYTDGFLTPD
                     VEAKRFYDHAQQYIYDNQLPDILADLATYSFQVFNVSQVSLQDPYNNISLNFDRPLYLK
                     RPFSIKTVSYNLIDIIDNDEIKRIEDLAVIPKKQTTYFFQKDYLNAISSNIFEIDDPIF
                     FRLLDNNLTGNEILNKADSLEEMEEWKNMICEIYDLEIIGMRD"
     gene            complement(5001..5852)
                     /locus_tag="CHX35_RS03725"
                     /old_locus_tag="SAMN05444972_102232"
     CDS             complement(5001..5852)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /locus_tag="CHX35_RS03725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_102232"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_091834118.1"
                     /sec_met_domain="DUF692 (E-value: 1.4e-31, bitscore: 104.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTYPHLGVGMLVHNSKTYMDYLDKLGDISDVYELLLIQYMNEPDE
                     LNKMADRLGKPIIAHTTRFSFGTDAPVERNLLDNMNDLIFRCKAKWCGDHLCFTGVPGL
                     GSGGLLPPIMNEESVDVFVRGILEAKKVINTPLLIENVVQYYQVGTMSQAQFLKDVVEK
                     ADIGIVLSMENISTSKSFMESDYYEFIDTIPVERIVQIHCTIGNSQEQKEMKSDIFGTI
                     YDKQMMQYEALEYLVKKRGVKPKAFIWELETETDSHPEVEELRQRMEWANSLFFKNKGA
                     GY"
     gene            6814..7230
                     /locus_tag="CHX35_RS03730"
                     /old_locus_tag="SAMN05444972_102233"
     CDS             6814..7230
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /locus_tag="CHX35_RS03730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_102233"
                     /product="hypothetical protein"
                     /protein_id="WP_176391867.1"
                     /transl_table=11
                     /translation="MAENLYPSVIQLCKTVRFDTSKIVQLHISLGELLLNQDKSEDTHD
                     VYESANALCEKEDYTGVKYIDLLIKIGDLFYKKDDNEKTIQYFEKALGLLNNNYLKYKY
                     AELFFKLSVCYAKIGSDKEIEYIRMYSNFRNPFN"
     misc_feature    complement(7272..7287)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 23.51"
                     /tool="antismash"
     misc_feature    7275..7290
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 24.65"
                     /tool="antismash"
     misc_feature    7277..7291
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 23.42"
                     /tool="antismash"
     misc_feature    complement(7278..7293)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.86"
                     /tool="antismash"
     gene            7335..7925
                     /locus_tag="CHX35_RS03735"
                     /old_locus_tag="SAMN05444972_102234"
     CDS             7335..7925
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1203: putative siderophore biosynthesis protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024913.4"
                     /locus_tag="CHX35_RS03735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_102234"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_091834123.1"
                     /transl_table=11
                     /translation="MKKNRILHSKYIDDLGLTMSIRKLDLINDFEVLHDWMHRPYISPF
                     WKLDVSNEDFSAYLKRTTSTPGREHFIFELDGEPFSFLLTYLVRNEDLKDHYDDFDIND
                     IGVHVVIGERSYIAEKYVVPYFQLGLDFVFKRNKESKKIIGEPDRRNRIIIPSAKKAGF
                     ISRGKITLPHKVANLIVADRIKFYERNEDETHV"
     gene            7918..8151
                     /locus_tag="CHX35_RS03740"
                     /old_locus_tag="SAMN05444972_102235"
     CDS             7918..8151
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="CHX35_RS03740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05444972_102235"
                     /product="hypothetical protein"
                     /protein_id="WP_091834125.1"
                     /transl_table=11
                     /translation="MFKEILDQEVFSVDITKRSAVCITSWQGEVKCMVDKDTLNKWKNA
                     KEELLQQEYNHRTDTMVLAVSEEEAVEKALSK"
     misc_feature    complement(8485..8495)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     misc_feature    8485..8497
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     misc_feature    8680..8692
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 20.68"
                     /tool="antismash"
     gene            8873..9163
                     /locus_tag="CHX35_RS03745"
     CDS             8873..9163
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /locus_tag="CHX35_RS03745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="trypsin-like peptidase domain-containing protein"
                     /protein_id="WP_091834128.1"
                     /transl_table=11
                     /translation="MGDLVAIGNPLGLNHTVTLGVISGKDRPLSMADRFFSRVIQTDAA
                     INPGNSGGPLLNIFGQAIGINTLVVYPSQSISFAIPIDEIKPLIRSYIEKT"
     gene            complement(9308..10366)
                     /locus_tag="CHX35_RS03750"
                     /old_locus_tag="SAMN05444972_102236"
     CDS             complement(9308..10366)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006285417.1"
                     /locus_tag="CHX35_RS03750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444972_102236"
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="WP_091834131.1"
                     /transl_table=11
                     /translation="MKKRFKILLLLICSPLVIVGGFLGYMTITDYVPPNSLSIPIEHNT
                     HQQLDVGVPFTVTSFNIGYAGLDKKEDFFMDGGTDSHGSSQEQVMKNLEGITQVLKKEN
                     SSFFFLQEVDIDSSRSFHVDELAHIKKHLPNKSVTFALNYTVPWVPVPVFDPHGSTQSG
                     LATLSEYKTTKSTRFSLYGKEAWPQQLFDLDRCIMENRVPLKNGKELLMVNVHLSAYDK
                     GGKVRKQQLQFLQEYMNKEYARGNYIVLGGDWNHQIPGTNSKRFKTTEAWPDWMQTIPS
                     NFAPSGFKWVADPTIPTSRTLARSYQKGVNFLSNIDGFIVSPNVKVMNTYGHSLGFEHS
                     DHNPVTTQLVLK"
     misc_feature    10432..10447
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.73"
                     /tool="antismash"
     misc_feature    complement(10434..10447)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 23.87"
                     /tool="antismash"
     misc_feature    complement(10789..10803)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.58"
                     /tool="antismash"
ORIGIN
        1 atcgccattg catctctaac caatttttct attttgtgac cccttacata tccttgtgct
       61 ccacaaagtt ggagaacctc attcgtcact tctttacata gttgtgctgc ccaaagctta
      121 gattgcatgg ttaatagtga agcacgcgga ttccgttgat ctgcaagatt tgcagcctcc
      181 aaaatcaaca attcaagtcc ctggattcgt gcatagaagt cagccaatat gaagcgcgta
      241 ttctgaaaac cccatatttt aggcttattc ttaatttcct cccgcaaaat atccagagca
      301 ttttgtgcaa ttcctaaacc aattatccca ggatgaattg cactggctcg attgatctcc
      361 atcatttctc ttccgcgccc tattcctcca agtatacgat caggataaat caaacagtta
      421 tcacaaataa tttcacgagt ggaggtcgaa cgtagtccca aaccatccca atcggctcca
      481 aataaaaccc cattgtcatc cttgcaaagt aagacaaaag ttaattcctc tccagaatta
      541 ttcccagttc taactagcac ggtatacaaa gaggagaacc cagcaccagt acagaaggct
      601 ttgctcccat ccaaaactaa tttaccttct ttccattttg cctttgtatt tagttctgac
      661 ttatctgcac ccgccttcag ttcactccaa gaagaggatg ccacccattc attagccaat
      721 agtggtaacc atttttcttt ctgttcagag gtaccaaaat ccaataatcg ctttactact
      781 tgattatgaa acatataaat tgttgctaat gaagggcagt cctttgctag tacaagggcg
      841 attttcgtca tctcaaggat atttccccct ttccctcccc attttttagg aattaatacc
      901 cccataagcc cttttctgag aagcgtttga atactcattt cgggatagct acctgccttt
      961 tcagaaatta tagcctccct gcgaattaaa tcgcttggta tcccccaatt ctgtttttct
     1021 tgtagattca tggcacattg actcccccaa ttttatatcc ttcttgtaat atacttacag
     1081 attctaattt tcctcctttc acatgcccag tagcctgtat cgttccccct ggaatctcta
     1141 tgaaataagg aagtgaaggc gtcatagtct tcccaacctg agtcataagt gtaccattcg
     1201 tatataatcc agcaattaaa ttagtcacac ctgttaatgc aaatctacca tgccaactag
     1261 gtacagtaat cattctagtt cgtatatgat taaacttaca cactggttta tcaacaacag
     1321 ctaccttagg aaatacagag tggcagttaa ttcctaactc tctctggact attgatctaa
     1381 tccgcatcaa taaggaatat tccctatgac tccattcaaa taaatccagt ggagacaagt
     1441 cacccgcacg tagccaaata tatgggtttc ctgcatttac agcagttact ttataatcca
     1501 tatctccatc ttttatttgc aaaatcggtt tattcgtact caataaattc tcattaatag
     1561 ttcgtacagg ttctttaaat gagatctgca ccctatgctg gttagaatat ctgcctaagt
     1621 aggttatttc tacaactttc ccagtattta atgatgtaaa ttttgcaagc ttatcaatat
     1681 cacctctatc tcgcaagaat aagtaagaag caactaaagc attgccacaa tcgccgcttt
     1741 cgtcaataag atcaaattcc ggaacatatt gagtaaactt gacaatataa tggtttttat
     1801 ctttgattga atgaataaat attaatttat tagtgggttt attaatcaaa ctactttcta
     1861 aatacacttt ccgggctatt tccaatggag aaagcccttc atggataatt tcatactcag
     1921 gaataataac cgcaattcga gtaaccccac tgatataaat gggaacaaac gtactttgtt
     1981 tataaatcaa cataactatc ccgccctttg atcttgataa agggaattga acgatccttt
     2041 ctgtaattga agaaatgttt caagtacaat aaaactccac agatgatggc tatagtctgc
     2101 acctttttgg tgcatattcc aaatcgataa tagtgaattt ttatgtacaa aattacaaat
     2161 tatggaattt gaagaaaaca aagtttcata aatccaattc ttccattgat cacgcatcca
     2221 accttgcatc ggaaaggtaa atggtttctt cttacgttct atcaaccaac gaggcataaa
     2281 ttgtgatgct tgatctttta accatacctt ctccactcca tctatacgga gatctcccgt
     2341 tgaaagcaat cgagcctgcg caatcataga gggtcgtagg aatggaactc gggcctcgac
     2401 tccccaagcc atggatagct tgtctactcg attgagatgg taacttggta aacgaaactt
     2461 ctgctcccac tccatggcgc cttttaaaga acgaacttga tttagttgtt gtattggttc
     2521 agcatatggc tcccaagcat tattctgtaa aattaggatc tcttttaatt gtgtatcact
     2581 gaaaagtagc agatcagaaa tatactgact ctgagttaca tccaatccaa cattcttgtc
     2641 ccaatggtga aaacgactat atcctccaaa aaattcatct gaaccatccc cagtcaacac
     2701 tactttgcag tgctctttga cttctctcat aagggcataa tacggtaagg taattggatc
     2761 ctgattaggc tcatccaaat gccagattac ttcttctagc atttctggaa cttctaaggg
     2821 atttaaaagg actgaatgaa aatgcccgtt tacttctttt gtcatctgct gagcatatat
     2881 ccgttcatcg tcctctgtct ctataggata gccgactgta aatacgggaa caggttcatc
     2941 catttgttct gccattgctt ttaggacaag attagagtct agtccaccac tagcaataca
     3001 tccaactttt acatcactca acatcgttgt acgtacttgt tcgtagaaaa attctttaaa
     3061 aggatctgta atggttgata catgtggctg aaagtacttt cttaccaatt tctgatcttg
     3121 tgttttataa atcaaaactt cgcctggttt tagtttccgg atctttttat acggcgtata
     3181 gggagttgga attgatttaa ataaaaggta actccctatc gccgagggat ctaactcaaa
     3241 cggcctttta tcaaattgat gaattgtttt tatctctgaa gcgaatacaa actcattatc
     3301 tgattgaatc gtatagtata aaggctttat tcctgctgaa tcaacactca ataacaatat
     3361 ttttttttca gcatcccaaa ttgcaagcgc aaacatccca tctaattctt caaacaagcg
     3421 ctcccctttt tcttcatata ggtgaggaag aatggctcca tcgctttgag aatcaatggt
     3481 atgtcctctg ctctctaacc aacaacgtaa ccgatcattg ttatagattt caccattgaa
     3541 tacgacaaca acattttttc gctcattata atacggttgc tcaccgaagg ctaaatcgcg
     3601 aatacttaat ctgcgcatac ccaaagccat aaagtcatct aaataaattc cttccgagtc
     3661 tggacctcga tgaactaaag agcccgtaca ttttttcagt tttacatagg attgataacg
     3721 atcacccttt atatgaaagc cgacgattcc acaaatttta aaaacccccc ttagaaaaaa
     3781 tacgcgggag atttatctcc cgcttttact accacttagt taccaccagc gtttttttca
     3841 acttttcctt ggttgttccg agtttgaact tttttcatga tattcacccc cttctatagc
     3901 actttatatt ggtcatttct tatagcactc tcaatgtctg attctcagca attaattacc
     3961 acttagttgc caccagcgtt tttttcaact tttccttggt tgttccgagt ttgaactttt
     4021 ttcatgatat tcaccctctt tcatagcact ttatattcgt catttcttat agcactctca
     4081 atgtctgatt ctcagcaatt aattaccact tagttgccac cagcgttttt ttcaactttt
     4141 ccttggttgt tccgagtttg aacttttttc atgatattca ccccctttca tagcactttt
     4201 tgttcagtcc ctcattccaa ttatttcgag atcatatatt tcacaaatca tgtttttcca
     4261 ttcttccatt tcctcgaggg aatcagcttt attaagaatc tcgtttccag ttaagttatt
     4321 atcaagtaat cgaaaaaaaa taggatcatc aatttcaaat atatttgagc ttatggcatt
     4381 caaataatcc ttttgaaaaa aatacgttgt ctgtttcttc ggaatcactg ccaaatcttc
     4441 tattctcttg atttcatcat tatcaataat gtcaattaaa ttgtatgaga ctgtttttat
     4501 agaaaaagga cgttttagat acaatggtct atcaaaattt aatgaaatat tattgtatgg
     4561 atcctgtaaa gatacttgtg atacattgaa tacctggaag gaatatgtag caaggtcagc
     4621 aagaatatct ggcaattgat tgtcataaat atattgctga gcatggtcat aaaatcgctt
     4681 agcctctaca tctggagtaa gaaacccatc tgtgtaacgt ttacagaatg aagaaaagaa
     4741 tgaatcaaat actcctacct gttgtagaac tgtggtaaat tcagcaaaat cagcgcgtct
     4801 ttttgccctt cgctcgcgat ctaattgatt tactgtacga tccagttgag tgaagtctaa
     4861 tgatacagct agttttaact cttctttgga aaagccaaat tcggaatacc gggtttcaat
     4921 tggcagtgat tttacttttt ctcgcaacga cttatctcta atataacgtg tcaaaaaggt
     4981 ttgtaattgt aatacaccca ttagtagccc gctcctttgt tcttaaagaa caatgaatta
     5041 gcccattcca ttcgttgacg taattcttcg acctctggat gggaatcagt ttctgtttcc
     5101 aactcccata taaacgcctt tggcttaact cccctcttct taaccaaata ttccagcgct
     5161 tcgtactgca tcatttgctt gtcataaata gtgccaaata tatcagattt catctctttc
     5221 tgttcctggc tgttgccaat tgtacaatga atctgaacta ttcgctcaac aggaatagta
     5281 tcaataaact cataataatc agattccatg aacgacttag aagtactgat attttccatg
     5341 ctaagtacaa ttccgatatc cgccttctct accacatctt taaggaattg agcttgagac
     5401 atagttccca cttgataata ttgcactacg ttttcgatta gtaatggtgt attgatcacc
     5461 tttttggctt ctaatatacc tcgaacaaaa acatcaacag attcttcatt cataatagga
     5521 ggaagcaaac caccagaacc taaacctgga actccagtaa aacatagatg atctccacac
     5581 cactttgcct tacatctaaa tattagatca ttcatgttat ctaataaatt tctttcaact
     5641 ggagcgtctg ttccaaaaga aaaccttgtt gtatgagcaa ttataggttt ccccagtcga
     5701 tcagccattt tgtttaactc gtctggctcg ttcatatact gaattaacag taactcgtaa
     5761 acgtctgata tatcacctaa tttatcaaga taatccatat aggtcttgga attatgaacg
     5821 agcataccga ctcctaaatg agggtaagtc attttatcat agtcactcct tcgtttaatt
     5881 tagttgctgg atgactcaga ctgtcaaaaa agagactgag catagtataa tcccgatctt
     5941 gacactttct tgctagttct atttcttggc tttttcagaa gtgaagttct aaatatagag
     6001 tatatgaaga caatgtttaa cattaataat catttaaagc atacaactct aagtaagatt
     6061 tgtaaaataa aaatatcaat atattttaag aagtcttttt aaaaaccaca tgaaactata
     6121 tcatacacac gaaaagtgtc tccagagagt gttgtgagca cgtggtttaa tcgtttactc
     6181 ccttctctcg ttccgttggg gttcgatgaa gggggcttga tcgtgttaaa ttgatacgcc
     6241 cccccgttgc ctcaggcgtc acatttgttg cttctatcac tccctattct tcccgtcttt
     6301 tagataatca cttaccagta tgcacaccta ctatatttag taatgatcac gcagttatta
     6361 tgtgatacgc attactctac ttcaaccaat tctagaccct taattcaata ccaaccctcc
     6421 aactatatgg ccaattataa tttaataaaa tgcaaaaatg caatataaat tatttacaaa
     6481 ttacaaaatt aaaatatttt tatcttttat aatttgagat attaacattt atgatctcat
     6541 gtctcaatac tttgcatcca acctcaaagc caccgtcatc aatttattgg ctaattttaa
     6601 tcaatactgc atttcactaa tttgaaacgt gagattaatt ttgatcatat ctcacaaaaa
     6661 aaacaatgga tttggacttg aaagcatttt aagaatgcta tttcaagtgc gaagatagga
     6721 ctttcttatt gtttgctttg ttcttgtttt gatagataca tagatatatg catgcttatg
     6781 tgtgatatat ttgcagatat gggcgaatta gaattggctg aaaatctgta tccttccgta
     6841 attcaacttt gcaaaacagt taggtttgat acatcaaaaa tagttcaatt acatatatca
     6901 ttaggtgaat tgttgctgaa tcaagataaa tccgaggata ctcatgatgt atatgagtct
     6961 gcaaatgctc tttgtgagaa agaagattat acaggtgtaa aatatattga cttattaata
     7021 aaaattggag acttgtttta taaaaaagat gataacgaaa aaaccatcca gtattttgaa
     7081 aaagctctcg gtctgctcaa taataattat cttaaatata aatacgctga actattcttc
     7141 aagttgtccg tatgctatgc taaaattgga agtgataaag aaatagaata tattagaatg
     7201 tacagtaatt ttagaaatcc ctttaattaa tttatttttt taattcctat ttaaaaaaat
     7261 aaattattgt tgataatgat aatccttttc aattataatt acgagtaact actctatcaa
     7321 aggatctgac gtgaatgaaa aaaaacagaa tacttcatag caaatatatt gatgatttgg
     7381 ggttaacgat gagtatccgt aaactagatc ttatcaatga ctttgaagtg ttacatgatt
     7441 ggatgcaccg cccttatata tcaccttttt ggaaacttga tgtatcaaac gaggattttt
     7501 ctgcttatct gaaaaggaca acatcaacac ctggacgaga acactttatt tttgaattag
     7561 atggtgagcc attctctttt ctactaacat acctggttag gaatgaagac ttaaaagatc
     7621 attatgatga ctttgatatt aacgatatcg gagtacatgt ggtgatcggt gagcgcagct
     7681 acatagcaga aaaatatgta gtcccttatt tccaattagg actcgatttt gtcttcaaga
     7741 gaaacaaaga gtcaaagaaa ataattggag aaccagatag aagaaacaga attattattc
     7801 cttctgctaa aaaagctgga tttatcagca ggggtaaaat tactctcccg cacaaagttg
     7861 caaatttaat tgttgccgat cgaatcaaat tttatgagag aaatgaggat gaaactcatg
     7921 tttaaggaaa ttcttgatca agaggtcttt tccgtggata ttacaaaaag gagtgcagtc
     7981 tgcataacat cttggcaagg tgaagtaaaa tgtatggtcg acaaggatac tttgaacaag
     8041 tggaaaaatg ctaaagagga attactacaa caggaatata accatagaac agataccatg
     8101 gtcctagccg tttcagaaga agaggcagtt gagaaagcct taagtaagtg aatataacat
     8161 ccatgaccta gttgaacaga aaaaggagga cgtcaatttg acgttctcct tactatttgc
     8221 attaataaag gattcaaatt aataatcctc ccatttgtat cctctctctc aatgtagcgc
     8281 catgatagag aaagatttct ggctaacggg gacggggatc atccatttac accatagtgt
     8341 ttttcctaga gaaatttcta cctcaacgcc tcttaaaccc gatatgtaaa gattaaaagc
     8401 acaaaacctt ggatgactct atccatccag ctttccagtg tttcattccg tgtggcgttg
     8461 taatttccca ctgtggatct tgtcgtgaca ttgtaaaaac taccagggtt tttctttgtc
     8521 ttgatccttt attttttagt attcgggtct tgcacgtttt ctgttccccc tccgcttttt
     8581 atgatatgct gttgtcccct ccggcttttg ccatcaggta agttacttga cccaaaggac
     8641 tctcctctat cctatccttc attgtgaagg ggttcagaat tgaattatgc aactcacaaa
     8701 ttagtttact taacattata cgtgtgattt ttccttttgt gatctttaac cctttagaga
     8761 tcatattcac aacggcactt ataatcaaca tccatattac tgtaatgaga gataatgata
     8821 tgggctaggc atctgtatca cagtaaagta aaatcccagt gttcgtttta atataggtga
     8881 tttagttgcc ataggtaacc ccctcggact taatcatacg gtcacccttg gtgtcatcag
     8941 tggcaaagac cgcccattaa gcatggccga ccgtttcttc agccgggtca ttcaaaccga
     9001 cgcagccatc aaccctggca atagcggagg acccctgctc aatatttttg ggcaagcaat
     9061 tggcatcaat acattagtgg tctacccctc ccaaagcatc agctttgcca tccccatcga
     9121 cgaaataaaa cccttaatac gttcgtatat tgagaagacc tagaagggat ccgcttaatt
     9181 taagcggatc ccttctataa aggaagactg ctgattaggc agtctttttc agggtaatca
     9241 tcagaggatc tttgatagca acacattaac cacttatcgc ccaaagcagg cgtgagccga
     9301 aagtgattta ttttaaaacc aattgtgtgg taaccgggtt atggtcacta tgttcaaatc
     9361 caagtgagtg tccataagtg ttcattactt taacattcgg ggaaacgata aagccgtcaa
     9421 tattggatag gaagtttact cctttttgat aagaacgggc tagcgttcta gaggtaggga
     9481 tcgtaggatc agctacccat ttaaagccgg atggagcaaa attactcgga atggtttgca
     9541 tccaatccgg ccaagcttct gtggttttaa aacgttttga attcgttcct ggaatttgat
     9601 gattccaatc gccacctagc acgatgtaat ttcctcgtgc atactctttg ttcatatact
     9661 cttggagaaa ttgaagttgt tgctttctca cctttccacc cttgtcgtat gcagagagat
     9721 gaacattcac cataagtaat tctttcccat ttttcaacgg aactcgattt tccatgatac
     9781 aacgatctaa atcaaacaac tgttgcggcc aagcttcttt tccatacagc gaaaagcgag
     9841 tgctctttgt tgttttgtac tctgatagag tggcgagccc tgattgggta gaaccgtgcg
     9901 gatcaaaaac aggcacagga acccaaggaa cggtatagtt gagtgcaaag gttacacttt
     9961 tattaggcaa gtgttttttt atatgagcta actcgtccac atgaaaactt ctagatgaat
    10021 caatatcgac ctcttggaga aagaagaagg aagagttctc tttctttaat acttgtgtga
    10081 tcccctctag attcttcatg acctgttctt gactactgcc atgagaatcc gttccaccat
    10141 ccataaaaaa gtcttctttt ttatcaagac ccgcatagcc gatattaaaa gaagtcactg
    10201 tgaaagggac tcccacatct aactgctgat gggtattatg ctcaatggga atagaaagag
    10261 aattgggtgg aacatagtcc gttatggtca tataaccgag aaaaccaccc acaataacaa
    10321 gtggactaca aattaatagc agcaatattt taaaccgctt tttcatatca tttcaatccc
    10381 tttgcaaaat atattattgt gtaatataca taatatccct atatcgacga tatttaaaat
    10441 aattatcgaa tataaatcca cttatcaatc cagatcgaaa gcaaaaatcc acccaatcga
    10501 atgatatggt tgtaggagca tgacagccgc ccatcttaaa agtctaagga atcttgggtt
    10561 tgaaccgcct ttctcagatg agctacttct tgcttttatc attttaaatt atcatgagtt
    10621 tatttattgt acaacaggta aaacctttca tttctagctg cattaaacgt gagagtctca
    10681 cgaaccagaa aggattgtag cacgctaact ccccttcgcc aaggagtgtc aacgaagccc
    10741 ttatgatagt gatttaggtg ctctcggtaa ttccctcatc taaaccatat cgtcactctt
    10801 gatgtcatca gtggcaaaga tcgtccctta aggatggaag atccttaagg ga
//
