LOCUS       NZ_FOYG01000026         1729 bp    DNA     linear   CON 22-AUG-2024
DEFINITION  Paenibacillus sp. cl130, whole genome shotgun sequence.
ACCESSION   NZ_FOYG01000026
VERSION     NZ_FOYG01000026.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus sp. cl130
  ORGANISM  Paenibacillus sp. cl130
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900116035.1-RS_2024_08_22
            Annotation Date                   :: 08/22/2024 03:12:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,232
            CDSs (total)                      :: 5,132
            Genes (coding)                    :: 5,055
            CDSs (with protein)               :: 5,055
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 8, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 8, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 40 of 77
            Pseudo Genes (incomplete)         :: 49 of 77
            Pseudo Genes (internal stop)      :: 13 of 77
            Pseudo Genes (multiple problems)  :: 20 of 77
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:52:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1729
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOYG01000026.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1729
                     /db_xref="taxon:1761876"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0073259_126"
                     /organism="Paenibacillus sp. cl130"
                     /strain="CL130"
     gene            <1..>1729
                     /locus_tag="BM358_RS26160"
                     /old_locus_tag="SAMN04488603_1261"
     region          1..1729
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1729
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1729
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[2:>1729](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3..>1729
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             3..>1729
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (1-53). E-value: 4.6e-18. Score:
                     57.2. Matches aSDomain: nrpspksdomains_BM358_RS26160_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (68-353). E-value:
                     5.6e-109. Score: 355.7. Matches aSDomain:
                     nrpspksdomains_BM358_RS26160_Condensation_LCL.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544418.1"
                     /locus_tag="BM358_RS26160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488603_1261"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_143109578.1"
                     /sec_met_domain="PP-binding (E-value: 2.8e-14, bitscore:
                     39.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.2e-86, bitscore:
                     275.7, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 6.9e-11, bitscore:
                     27.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGVHDNFFDIGGHSLRATTLVSKIHKELNVDLPLRDVFRHSMIE
                     SMASAISRLDEQTFIAIPVADDREVYPQSFAQKRLFILNQLEGAELSYNMPEAMLLEGA
                     LDRTRFEEAFRKLVARHEMLRTGFEMVDGEASQRLYHDVNFAVEFYRVDEQEAEETVRR
                     FVRPFDLVKPPLLRVGLVELAPERHILMYDMHHIISDGVSMEIFVEEFVRLYGGEQLEP
                     LRVQYKDYTVWQHSQEQKERLQRQEAYWLDMFQGELPVLEMPTDYPRPAVQSYEGQTLE
                     FFFDASKTDGLKQLASETGTTLFMVLLAAYNVLLHKYSGQEDVIVGTPIAGRNHGDVQP
                     LIGIFLNTLAIRSYPASEKTFLSYLNEVKETTLHAFEHQNYPFEELVDKVQVTRDLSRN
                     PLFDTLFTMQNTEDEEFELEGLRLIPYPSALDTAKFDISLDVGEENGGLDYSFEYATAL
                     YKRETIERLAKHYEQLLVTIVSRPDAKIAELNLLTAEEKEQILGAFNPAQPEAAPVAAF
                     HRLFEEQAERTPEAEAVVYENDRLTYAELNERANRLAATLRASGIGRETIVGILAERSV
                     "
     aSDomain        7..162
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM358_RS26160_PCP.1"
                     /evalue="4.60E-18"
                     /label="BM358_RS26160_PCP.1"
                     /locus_tag="BM358_RS26160"
                     /protein_end="53"
                     /protein_start="1"
                     /score="57.2"
                     /tool="antismash"
                     /translation="IGVHDNFFDIGGHSLRATTLVSKIHKELNVDLPLRDVFRHSMIES
                     MASAISR"
     aSDomain        208..1062
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM358_RS26160_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.60E-109"
                     /label="BM358_RS26160_Condensation_LCL.1"
                     /locus_tag="BM358_RS26160"
                     /protein_end="353"
                     /protein_start="68"
                     /score="355.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIISDG"
                     /tool="antismash"
                     /translation="EVYPQSFAQKRLFILNQLEGAELSYNMPEAMLLEGALDRTRFEEA
                     FRKLVARHEMLRTGFEMVDGEASQRLYHDVNFAVEFYRVDEQEAEETVRRFVRPFDLVK
                     PPLLRVGLVELAPERHILMYDMHHIISDGVSMEIFVEEFVRLYGGEQLEPLRVQYKDYT
                     VWQHSQEQKERLQRQEAYWLDMFQGELPVLEMPTDYPRPAVQSYEGQTLEFFFDASKTD
                     GLKQLASETGTTLFMVLLAAYNVLLHKYSGQEDVIVGTPIAGRNHGDVQPLIGIFLNTL
                     AIRS"
     CDS_motif       277..387
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26160_0001"
                     /evalue="5.70E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="BM358_RS26160"
                     /protein_end="128"
                     /protein_start="91"
                     /score="54.1"
                     /tool="antismash"
                     /translation="SYNMPEAMLLEGALDRTRFEEAFRKLVARHEMLRTGF"
     CDS_motif       583..648
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26160_0002"
                     /evalue="9.70E-11"
                     /label="C3_LCL_132-143"
                     /locus_tag="BM358_RS26160"
                     /protein_end="215"
                     /protein_start="193"
                     /score="34.1"
                     /tool="antismash"
                     /translation="MHHIISDGVSMEIFVEEFVRLY"
     CDS_motif       679..711
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26160_0003"
                     /evalue="1.40E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="BM358_RS26160"
                     /protein_end="236"
                     /protein_start="225"
                     /score="14.5"
                     /tool="antismash"
                     /translation="QYKDYTVWQHS"
     CDS_motif       970..1059
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26160_0004"
                     /evalue="3.10E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="BM358_RS26160"
                     /protein_end="352"
                     /protein_start="322"
                     /score="42.1"
                     /tool="antismash"
                     /translation="DVIVGTPIAGRNHGDVQPLIGIFLNTLAIR"
     CDS_motif       1108..1227
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26160_0005"
                     /evalue="1.30E-20"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BM358_RS26160"
                     /protein_end="408"
                     /protein_start="368"
                     /score="65.8"
                     /tool="antismash"
                     /translation="KETTLHAFEHQNYPFEELVDKVQVTRDLSRNPLFDTLFTM"
ORIGIN
        1 ggagattggc gttcacgaca acttcttcga catcggcggc cactccctgc gggcgacgac
       61 gctggtcagc aagattcata aagagttgaa cgtggatctg ccgctgcgtg acgtgttccg
      121 ccattccatg atcgagagca tggcatccgc catttcccgg ctggatgagc agacattcat
      181 tgccattccg gtggcggatg accgggaggt gtacccgcaa tcttttgctc aaaaacgtct
      241 ctttatcctg aatcaactgg aaggcgcgga gcttagctac aacatgccgg aagcgatgct
      301 gctggagggt gctttggatc ggacaaggtt cgaagaagcg ttccgtaagc tcgtggcgcg
      361 gcatgaaatg ctgcgcaccg ggttcgaaat ggtggatggc gaagcatcgc agcggttata
      421 ccatgacgtg aattttgctg tggagttcta tcgagtggat gagcaagagg ccgaagagac
      481 ggttcgccgt ttcgtccgtc cgtttgactt ggtgaagcct ccgctgctga gggtaggcct
      541 tgtcgagctg gctccggaac gccatattct aatgtacgac atgcatcata ttatttccga
      601 cggcgtctcg atggaaatct ttgttgaaga attcgtccgc ttgtacggcg gcgagcaatt
      661 ggagcctctg cgcgttcagt acaaagacta caccgtttgg cagcattcgc aggagcagaa
      721 ggaacggctt cagcgtcagg aggcgtactg gctggacatg ttccaaggcg agcttccggt
      781 gctggaaatg ccgaccgact atccgcgtcc ggccgtgcag agctacgaag gccaaacgct
      841 ggagtttttc ttcgacgctt cgaaaaccga cggcctgaag cagctggcct cggaaacggg
      901 cacaacgctg tttatggtgc tgcttgcggc gtataacgtc cttctgcata aatattcagg
      961 tcaggaagac gtgatcgtcg gtacgccgat tgccggaagg aatcatgggg atgtgcagcc
     1021 gttgatcggg attttcttaa acacgctggc tatccgcagt tatccggctt cggagaagac
     1081 attcctgtca tacctgaacg aagtcaaaga aacgaccctc cacgccttcg agcatcaaaa
     1141 ctatccgttc gaagaattgg tggacaaggt gcaagtcacc cgtgatttaa gccgcaatcc
     1201 gctcttcgac acgctgttta cgatgcagaa tacggaggac gaggaatttg agctggaagg
     1261 gcttcgcctg attccttatc cgagcgcact ggataccgcg aagtttgata tcagcttgga
     1321 tgtgggcgag gagaacggcg gcttggatta cagcttcgaa tatgcgacgg ctctctacaa
     1381 aagggagacg atcgaacggc tggcgaaaca ttacgagcag ctgctcgtaa cgatcgtaag
     1441 ccgtccagat gcgaagatcg ccgagctgaa cttgctgacg gcagaggaaa aagaacaaat
     1501 tctcggcgcg ttcaacccag cgcagccgga agcggctcct gtggccgcgt tccaccggct
     1561 gttcgaggaa caggcggagc gcacgccgga agcggaggcc gtcgtgtacg agaacgaccg
     1621 gctgacgtat gcggagctga acgagcgggc gaaccgtttg gcggccacgc tgcgcgcaag
     1681 cggcatcggc cgggagacga tcgtcggcat tctcgccgag cgttcggtg
//
