LOCUS       NZ_FOYG01000024         1994 bp    DNA     linear   CON 22-AUG-2024
DEFINITION  Paenibacillus sp. cl130, whole genome shotgun sequence.
ACCESSION   NZ_FOYG01000024
VERSION     NZ_FOYG01000024.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus sp. cl130
  ORGANISM  Paenibacillus sp. cl130
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900116035.1-RS_2024_08_22
            Annotation Date                   :: 08/22/2024 03:12:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,232
            CDSs (total)                      :: 5,132
            Genes (coding)                    :: 5,055
            CDSs (with protein)               :: 5,055
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 8, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 8, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 40 of 77
            Pseudo Genes (incomplete)         :: 49 of 77
            Pseudo Genes (internal stop)      :: 13 of 77
            Pseudo Genes (multiple problems)  :: 20 of 77
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:52:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1994
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOYG01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1994
                     /db_xref="taxon:1761876"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0073259_124"
                     /organism="Paenibacillus sp. cl130"
                     /strain="CL130"
     gene            complement(<1..>1994)
                     /locus_tag="BM358_RS26145"
                     /old_locus_tag="SAMN04488603_1241"
     region          1..1994
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1994
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1994
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1994](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1994)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..1994)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (127-195). E-value: 4.9e-25. Score:
                     79.5. Matches aSDomain: nrpspksdomains_BM358_RS26145_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (211-495). E-value:
                     5.9e-107. Score: 349.0. Matches aSDomain:
                     nrpspksdomains_BM358_RS26145_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544418.1"
                     /locus_tag="BM358_RS26145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488603_1241"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_231517255.1"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-17, bitscore:
                     49.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2e-18, bitscore:
                     53.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 3.9e-89, bitscore:
                     284.7, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLPDGNIEYLGRIDHQVKIRGYRIELDEIETQLLNVEGVEEAVVL
                     ARQDGGGEKALVAYFVADRMLTVSEMRTSLAQGMPGYMIPSYFVQLERMPLTTNGKVDR
                     KALPEPQGGIQTGVEYVAPRNRTESQLVKIWEEVLGYSGIGVLDNFFELGGHSLRATNL
                     VSKIQKEMNVELPLRDVFRYSTIEEMALAISRIGEQSFSSIPLAGARAYYPLSSAQKRL
                     FILNQLEGADQSYNMPGVLLLEGSIDRSLLEKAFRGLIARHETLRTGFEIVQGEAIQRI
                     YESVDFAVEYRHASEEEAPEVVQDFIRPFDLAKPPLLRAELVELAAERYLLMFDMHHIV
                     SDGVSMDVLVEELVRLYGGESLEPLHIQYKDYAVWQQSDEQKVQLKREEAYWLDRYRGE
                     LPVLEMPTDYPRPAVQSFEGQTLTSFVDEATNEGLKQLAAQRGTTLYMVLLAAYTVLLH
                     KYTGQDDLIVGTSIAGRTHGDTQPLIGMFVNTLALRNYPASEKTFLSYLEEVKETTLGA
                     YEHQNYPFEELVDKVQVSRDLSRNPLFDTMFSLQNLEDKEFELEGLKLSPYPSEYGTAK
                     FDLSVDVTEENGGLECSFEFATALYKESTIRRLSTHFGHLLAAIVSCPDAKIAELNLLT
                     AEEKEQILGAFNPAQPEAAPVAAFHRLFE"
     CDS_motif       complement(345..464)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26145_0007"
                     /evalue="5.10E-23"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BM358_RS26145"
                     /protein_end="550"
                     /protein_start="510"
                     /score="73.5"
                     /tool="antismash"
                     /translation="KETTLGAYEHQNYPFEELVDKVQVSRDLSRNPLFDTMFSL"
     aSDomain        complement(510..1361)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM358_RS26145_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.90E-107"
                     /label="BM358_RS26145_Condensation_LCL.1"
                     /locus_tag="BM358_RS26145"
                     /protein_end="495"
                     /protein_start="211"
                     /score="349.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVSDG"
                     /tool="antismash"
                     /translation="YYPLSSAQKRLFILNQLEGADQSYNMPGVLLLEGSIDRSLLEKAF
                     RGLIARHETLRTGFEIVQGEAIQRIYESVDFAVEYRHASEEEAPEVVQDFIRPFDLAKP
                     PLLRAELVELAAERYLLMFDMHHIVSDGVSMDVLVEELVRLYGGESLEPLHIQYKDYAV
                     WQQSDEQKVQLKREEAYWLDRYRGELPVLEMPTDYPRPAVQSFEGQTLTSFVDEATNEG
                     LKQLAAQRGTTLYMVLLAAYTVLLHKYTGQDDLIVGTSIAGRTHGDTQPLIGMFVNTLA
                     LRN"
     CDS_motif       complement(513..602)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26145_0006"
                     /evalue="3.90E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="BM358_RS26145"
                     /protein_end="494"
                     /protein_start="464"
                     /score="44.9"
                     /tool="antismash"
                     /translation="DLIVGTSIAGRTHGDTQPLIGMFVNTLALR"
     CDS_motif       complement(861..893)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26145_0005"
                     /evalue="2.10E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="BM358_RS26145"
                     /protein_end="378"
                     /protein_start="367"
                     /score="14.0"
                     /tool="antismash"
                     /translation="QYKDYAVWQQS"
     CDS_motif       complement(924..989)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26145_0004"
                     /evalue="9.50E-11"
                     /label="C3_LCL_132-143"
                     /locus_tag="BM358_RS26145"
                     /protein_end="357"
                     /protein_start="335"
                     /score="34.1"
                     /tool="antismash"
                     /translation="MHHIVSDGVSMDVLVEELVRLY"
     CDS_motif       complement(1185..1295)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26145_0003"
                     /evalue="2.30E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="BM358_RS26145"
                     /protein_end="270"
                     /protein_start="233"
                     /score="52.1"
                     /tool="antismash"
                     /translation="SYNMPGVLLLEGSIDRSLLEKAFRGLIARHETLRTGF"
     aSDomain        complement(1410..1613)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM358_RS26145_PCP.1"
                     /evalue="4.90E-25"
                     /label="BM358_RS26145_PCP.1"
                     /locus_tag="BM358_RS26145"
                     /protein_end="195"
                     /protein_start="127"
                     /score="79.5"
                     /tool="antismash"
                     /translation="ESQLVKIWEEVLGYSGIGVLDNFFELGGHSLRATNLVSKIQKEMN
                     VELPLRDVFRYSTIEEMALAISR"
     CDS_motif       complement(1521..1550)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26145_0002"
                     /evalue="4.10E-03"
                     /label="PCP_mC"
                     /locus_tag="BM358_RS26145"
                     /protein_end="158"
                     /protein_start="148"
                     /score="10.5"
                     /tool="antismash"
                     /translation="NFFELGGHSL"
     CDS_motif       complement(1905..1970)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26145_0001"
                     /evalue="2.00E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="BM358_RS26145"
                     /protein_end="30"
                     /protein_start="8"
                     /score="38.9"
                     /tool="antismash"
                     /translation="YLGRIDHQVKIRGYRIELDEIE"
ORIGIN
        1 tcctcgaaca gccggtggaa cgctgccaca ggagccgctt ccggctgcgc cgggttaaac
       61 gcgccgagaa tttgctcttt ttcctctgcc gtcaacaagt tcagctcggc aatcttcgca
      121 tccggacagc ttacgatcgc cgcaagcaaa tgtccgaaat gagtcgacag ccgccggatc
      181 gtgctttctt tataaagagc cgttgcgaat tcaaaactgc actccaggcc gccgttttct
      241 tccgtaacat ccacactcag gtcgaacttg gccgtgccgt attcgctagg gtacggggac
      301 aatttcagcc cttccagctc aaactcttta tcctccaagt tttgtaggga gaacatcgtg
      361 tcaaacagcg ggttgcggct caaatcccgg ctgacctgca ctttatcaac gagctcttcg
      421 aacggataat tctgatgctc gtaagcgcct aaggtcgttt ctttcacttc ttccagatac
      481 gacaggaagg tcttctccga agccggataa ttgcggagcg ccagcgtatt gacgaacatt
      541 ccgatcaaag gctgcgtgtc tccgtgcgtt ctgcccgcaa tcgacgttcc gacgatcaaa
      601 tcgtcctgac ctgtgtattt atgcaaaagc acggtatatg ccgcaagcag caccatatac
      661 agcgtcgttc ctctttgagc ggccagctgc ttcaagcctt cgttcgttgc ctcgtccacg
      721 aaggacgtca gcgtttgtcc ctcaaagctc tgcacggcag gacgcggata gtccgtcggc
      781 atttccagaa ccggcagctc gccccggtaa cggtccagcc agtaagcttc ctcgcgtttc
      841 aactgcactt tctgctcgtc cgactgctgc cataccgcat agtccttgta ttgaatgtgc
      901 aaaggctcta atgactcgcc gccgtacaga cgaacgagtt cctcgactaa cacgtccatc
      961 gaaaccccgt cggagacgat atggtgcatg tcgaacatca gcaaataacg ttcggctgcc
     1021 agctctacga gctccgcccg cagcagcgga ggcttcgcca agtcgaaagg ccggatgaag
     1081 tcctgcacga cttcaggcgc ttcttcctcg ctcgcatgac ggtactcgac ggcaaagtcg
     1141 acgctctcgt aaatgcgctg tattgcttcg ccttgtacga tctcaaagcc ggttcgcagt
     1201 gtttcgtgcc gtgcgatcag tccgcggaaa gccttctcca gcaggctccg gtcaatcgat
     1261 ccttccagca gcagcacgcc cggcatgttg tagctctgat cggccccttc cagctgattc
     1321 aggataaaca gccgcttctg agctgaggaa agcggataat atgctcttgc gcctgccagc
     1381 ggaatcgacg agaacgactg ctctccgatc cgggagatgg caagagccat ctcttcgatc
     1441 gtcgagtagc ggaatacgtc gcgcagaggc agctcgacgt tcatttcctt ctgaatcttg
     1501 ctgacaaggt tcgtcgcccg caaggagtgg cctccgagct cgaagaaatt gtccaggact
     1561 ccaatgccag agtagcccag cacttcctcc cagatcttca caagctggga ctccgtccgg
     1621 ttacgcggcg ctacatattc gacgcccgtt tgtatgccgc cttgcggctc cggcagcgct
     1681 ttgcggtcca ctttgccgtt ggtcgtcagc ggcatacgct ccaactgcac gaagtacgac
     1741 gggatcatgt accccggcat tccctgggcc agcgaggttc tcatttcgct gaccgtcagc
     1801 attcggtccg ccacaaagta ggcgacaagc gccttctcgc cccccccgtc ctgacgggcg
     1861 agcaccaccg cttcttccac gccctcgacg ttcagcagct gcgtctcgat ttcgtccagc
     1921 tcgatccggt acccgcggat tttgacctgg tggtcgatcc gtcccaggta ttcgatgttg
     1981 ccgtccggca gcca
//
