LOCUS       NZ_FOYG01000023         2069 bp    DNA     linear   CON 22-AUG-2024
DEFINITION  Paenibacillus sp. cl130, whole genome shotgun sequence.
ACCESSION   NZ_FOYG01000023
VERSION     NZ_FOYG01000023.1
KEYWORDS    WGS; RefSeq.
SOURCE      Paenibacillus sp. cl130
  ORGANISM  Paenibacillus sp. cl130
            Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae;
            Paenibacillus.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900116035.1-RS_2024_08_22
            Annotation Date                   :: 08/22/2024 03:12:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,232
            CDSs (total)                      :: 5,132
            Genes (coding)                    :: 5,055
            CDSs (with protein)               :: 5,055
            Genes (RNA)                       :: 100
            rRNAs                             :: 7, 8, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 8, 5 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 40 of 77
            Pseudo Genes (incomplete)         :: 49 of 77
            Pseudo Genes (internal stop)      :: 13 of 77
            Pseudo Genes (multiple problems)  :: 20 of 77
            CRISPR Arrays                     :: 5
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:52:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2069
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOYG01000023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2069
                     /db_xref="taxon:1761876"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0073259_123"
                     /organism="Paenibacillus sp. cl130"
                     /strain="CL130"
     gene            <1..>2069
                     /locus_tag="BM358_RS26140"
                     /old_locus_tag="SAMN04488603_1231"
     region          1..2069
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2069
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2069
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>2069](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>2069
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>2069
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (87-156). E-value: 1.4e-24. Score:
                     78.0. Matches aSDomain: nrpspksdomains_BM358_RS26140_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (171-456). E-value:
                     2.2e-109. Score: 357.0. Matches aSDomain:
                     nrpspksdomains_BM358_RS26140_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312094.1"
                     /locus_tag="BM358_RS26140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488603_1231"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_143109576.1"
                     /sec_met_domain="PP-binding (E-value: 3e-20, bitscore:
                     58.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 8.1e-90, bitscore:
                     287.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 6.3e-15, bitscore:
                     40.6, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVVVVREDQEGQKALCAYYTAEEGLTAADLKRAIASELPGYMIP
                     SYFVELERLPLTPNGKIDRKALPAPEGGAGGGREYVAPRTELEAKLAAIWQDVLVREKA
                     VGVTDNFFDLGGHSLRATTLVSKMHKELGVEFPLRDVFRYSTVEEMAAAMERLEIGSFT
                     AIPAAEPSEYYPLSSAQKRLYILNQLEGAELSYNIPGAMLLEGELDRQRFEEAFRGLVA
                     RHETLRTGFEMVKGEAVQRIYEEAAFQVEYVQISGEQAEETVRQFVRPFDLAKPPLLRV
                     GLAELAPDRHILMFDTHHIVSDGVSIDVLIEEFVRLYSGEQLEPLRIQYKDYAVWQQSD
                     VQKAQLAKQEAYWLDMFRGELPVLELSTDYPRPAMQSYEGRTLQLFMHSEKSEGLKRLA
                     AENGATLYMVLLAGYTILLHKYTGQEDVVVGTPIAGRNHSDVQPLIGMFVNTLAIRSYP
                     AAGKTFLDYLKEIKETTLGAFEHQNYPFEELVDKVNVARDLRRNPLFDTMFALQNTENL
                     EIQLPGLHLSTYASEEIVSKFDLSLDVTEIEEGLEYLFEYATALYKTETVEKLAAHYLQ
                     LLESILRNPSATIAELGILTPAEKEQILGAFNPAQPEAAPTAAFHRLFEEQAECTPEEA
                     AVVYENDRLTYAELNERANRLAATLRASGIGRETIVGILAERSVDLLVAVLAVW"
     aSDomain        262..468
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM358_RS26140_PCP.1"
                     /evalue="1.40E-24"
                     /label="BM358_RS26140_PCP.1"
                     /locus_tag="BM358_RS26140"
                     /protein_end="156"
                     /protein_start="87"
                     /score="78.0"
                     /tool="antismash"
                     /translation="EAKLAAIWQDVLVREKAVGVTDNFFDLGGHSLRATTLVSKMHKEL
                     GVEFPLRDVFRYSTVEEMAAAMER"
     aSDomain        514..1368
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM358_RS26140_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.20E-109"
                     /label="BM358_RS26140_Condensation_LCL.1"
                     /locus_tag="BM358_RS26140"
                     /protein_end="456"
                     /protein_start="171"
                     /score="357.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVSDG"
                     /tool="antismash"
                     /translation="EYYPLSSAQKRLYILNQLEGAELSYNIPGAMLLEGELDRQRFEEA
                     FRGLVARHETLRTGFEMVKGEAVQRIYEEAAFQVEYVQISGEQAEETVRQFVRPFDLAK
                     PPLLRVGLAELAPDRHILMFDTHHIVSDGVSIDVLIEEFVRLYSGEQLEPLRIQYKDYA
                     VWQQSDVQKAQLAKQEAYWLDMFRGELPVLELSTDYPRPAMQSYEGRTLQLFMHSEKSE
                     GLKRLAAENGATLYMVLLAGYTILLHKYTGQEDVVVGTPIAGRNHSDVQPLIGMFVNTL
                     AIRS"
     CDS_motif       583..693
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26140_0001"
                     /evalue="3.40E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="BM358_RS26140"
                     /protein_end="231"
                     /protein_start="194"
                     /score="54.8"
                     /tool="antismash"
                     /translation="SYNIPGAMLLEGELDRQRFEEAFRGLVARHETLRTGF"
     CDS_motif       892..954
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26140_0002"
                     /evalue="2.00E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="BM358_RS26140"
                     /protein_end="318"
                     /protein_start="297"
                     /score="29.9"
                     /tool="antismash"
                     /translation="HHIVSDGVSIDVLIEEFVRLY"
     CDS_motif       985..1017
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26140_0003"
                     /evalue="6.90E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="BM358_RS26140"
                     /protein_end="339"
                     /protein_start="328"
                     /score="15.4"
                     /tool="antismash"
                     /translation="QYKDYAVWQQS"
     CDS_motif       1276..1365
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26140_0004"
                     /evalue="5.70E-17"
                     /label="C5_LCL_267-296"
                     /locus_tag="BM358_RS26140"
                     /protein_end="455"
                     /protein_start="425"
                     /score="54.0"
                     /tool="antismash"
                     /translation="DVVVGTPIAGRNHSDVQPLIGMFVNTLAIR"
     CDS_motif       1414..1533
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM358_RS26140_0005"
                     /evalue="2.20E-21"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BM358_RS26140"
                     /protein_end="511"
                     /protein_start="471"
                     /score="68.3"
                     /tool="antismash"
                     /translation="KETTLGAFEHQNYPFEELVDKVNVARDLRRNPLFDTMFAL"
ORIGIN
        1 gaagcggtcg tcgtcgtcag ggaagatcaa gaaggtcaga aagctttgtg cgcttattat
       61 acagcggaag aaggcttgac ggcggcggac ctgaagcggg cgattgccag cgagctgccg
      121 gggtacatga tcccgtcgta cttcgtggag ctggagcgcc tgcctctgac gccgaacgga
      181 aagatcgacc ggaaggcgct gccggcaccg gaagggggag caggcggagg ccgcgaatac
      241 gtggcgccgc gcaccgaact ggaggcgaag ctggccgcca tttggcagga tgtgcttgtt
      301 cgggagaagg cggtaggcgt aacggacaac ttcttcgacc tcggcggaca ctccctgcgg
      361 gcgacgacgc ttgtcagcaa aatgcataag gagctaggcg ttgaattccc gcttcgcgac
      421 gtattccgct actcgacggt tgaggaaatg gccgcggcta tggagcggct ggagatcggc
      481 tcgttcacgg ctattccggc tgcggaacct agcgagtatt atccgctctc ttccgctcag
      541 aaacgtctct atatcttgaa ccagctggaa ggagccgagc tgagctacaa cataccggga
      601 gctatgctgc tcgaagggga gctcgaccgg cagcggtttg aagaagcgtt ccgggggctc
      661 gtagctcgtc atgaaacgct gcgtaccggc tttgagatgg taaaaggcga agcggttcag
      721 cggatttacg aagaagccgc cttccaggtg gaatatgtgc agatcagcgg ggagcaggcg
      781 gaagaaacgg tgcgccaatt cgttcgtccg tttgatctag cgaagccgcc acttctgcgg
      841 gtaggccttg ccgaactggc gccggaccgg cacattctaa tgttcgatac gcatcatatc
      901 gtatctgacg gcgtttcgat tgacgtactg atcgaagagt tcgtccgctt gtacagcggg
      961 gagcagttgg agccgctccg cattcagtac aaagattatg cggtatggca gcaatcggac
     1021 gtgcagaaag ctcagcttgc caagcaggaa gcctactggc tcgacatgtt ccgcggagag
     1081 ctgccggttt tggaattgtc gacggactat ccgcgcccgg ctatgcagag ctacgagggc
     1141 cgcacgctgc aattgtttat gcatagcgag aaaagcgagg gtctgaaacg gcttgcagcc
     1201 gagaacggcg caacgcttta catggttctg cttgctggtt atacaatatt attgcataaa
     1261 tatacagggc aagaagacgt ggtggtcggt acgccgattg cgggaagaaa tcatagcgac
     1321 gttcagccgc tgatcgggat gttcgtcaat accctggcca tccgcagtta tccggctgcc
     1381 ggtaagacgt tccttgacta cttgaaggaa atcaaggaga cgacgctggg cgcttttgaa
     1441 catcagaatt atccgtttga ggaactggtg gataaggtga acgtggctcg tgatttacgc
     1501 cgcaatccgc tgttcgatac gatgtttgct ttgcagaata cagagaattt ggaaatccag
     1561 cttcccggac tccatttgtc gacgtatgcc agcgaagaaa ttgtttctaa attcgatctc
     1621 agcttggacg tcacggagat cgaggaaggc ttggaatatc tgtttgaata cgccactgct
     1681 ctttataaaa ccgaaacggt ggagaaattg gccgctcact acttgcagct gcttgaatcc
     1741 attcttcgca acccatcggc gactattgcc gagctgggca ttttgacacc ggcggaaaaa
     1801 gagcaaattc tcggcgcgtt taacccggcg cagccggagg cggctcctac ggccgcgttc
     1861 caccggctgt ttgaggaaca ggcggagtgc acgccggaag aggcggccgt cgtgtacgag
     1921 aatgaccggc tgacctatgc ggagctgaac gagcgggcga atcgcttggc ggctacgctg
     1981 cgcgcaagcg gcatcggccg ggagacgatc gtcggcattc tcgccgagcg ttcggtggac
     2041 ttgctggtgg ccgtgctggc cgtctggaa
//
