LOCUS       NZ_FOYL01000030        11260 bp    DNA     linear   CON 09-DEC-2024
DEFINITION  Lentzea waywayandensis strain DSM 44232, whole genome shotgun
            sequence.
ACCESSION   NZ_FOYL01000030
VERSION     NZ_FOYL01000030.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lentzea waywayandensis
  ORGANISM  Lentzea waywayandensis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Lentzea.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900115955.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 15:54:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,449
            CDSs (total)                      :: 9,363
            Genes (coding)                    :: 9,244
            CDSs (with protein)               :: 9,244
            Genes (RNA)                       :: 86
            rRNAs                             :: 5, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 4, 6 (16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 26 of 119
            Pseudo Genes (incomplete)         :: 101 of 119
            Pseudo Genes (internal stop)      :: 5 of 119
            Pseudo Genes (multiple problems)  :: 12 of 119
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:39:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6949
            Orig. end                         :: 18209
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOYL01000030.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11260
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11260
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11260
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:6260](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6260)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    433..448
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.46"
                     /tool="antismash"
     gene            848..2491
                     /locus_tag="BM417_RS46405"
                     /old_locus_tag="SAMN04488564_1307"
     CDS             848..2491
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055: pyruvate oxidase/decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014794.5"
                     /locus_tag="BM417_RS46405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488564_1307"
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="WP_093606513.1"
                     /sec_met_domain="TPP_enzyme_N (E-value: 4e-29, bitscore:
                     91.7, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 5.5e-34, bitscore:
                     107.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIAAAPRTTLAEQVPELLGLLGVTAGFGIPGGTVAPTWDAIARS
                     PLELLHFRHECGAVFAAVEAYFATGAPVAVCVTGGPGITNAITGLHAARSDGAKVLLVS
                     GATPKWSWGRHPFQETSPAALGADMFTEGPLFHYAALVDNELTDVVAPLAAGMRGPGGF
                     VAHLSLTRDGQLSGATRPLRLPPDSPQAPPDPAMVALVADFLRAEHMTLWLGFGARHAA
                     SEIRELVERTGTRVLCSPRAKGIFPERHPAFVGVTGLAGGAFTDHPHRMLVLGSGLGEM
                     TSFWSPDLVPPRGFVHVDLDEDVPGSAYPGVPLIPVHCEIRAFLREVLDALPARTAEPP
                     PGRRARPVEAPRSGPVRPQVLMAAVQRVIVDGSDATVLAEAGNSFLWAGRHLEFDTARY
                     RVSTGFGAMGHAATGVLGAAHAVDGKAVAIVGDGALLMNNEISTAVQYQIPAVWVVLND
                     SAYGMVRQGMTAEGLRPEGVRMPRTDFVGLARAMGADGITVTDETELDQALAVALAAPG
                     PFVLDVLVDDTCSAPMGERVASLTSQRMEL"
     gene            2494..3576
                     /locus_tag="BM417_RS46410"
                     /old_locus_tag="SAMN04488564_1308"
     CDS             2494..3576
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF020130.5"
                     /locus_tag="BM417_RS46410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488564_1308"
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="WP_093606522.1"
                     /sec_met_domain="fabH (E-value: 4e-38, bitscore: 121.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 8e-09, bitscore:
                     25.6, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPGGVGIRSLAVAFPASVRTNDYFRENYPELVATAESRSLAQVFS
                     PQDEKVRLDTWEREMAPYLADPFRGAVERRILAPGETALDLEARAARRALDAAGLDVAD
                     VDLAIVTSFMPDQLGPGNAAYLARELGMRGTAWNVESACSSGLVSLETATALVEAGRYE
                     IVLVVISCTYSRVSDYSDTLAWFWGDGAGAFVVGRTPEGVGVMGSMSTNTASTCDTFSN
                     HIVVDEHGEPHIRTTASKATGRILRDTAEASLRETCEGALACAGVDLLDIDFFVCNSPV
                     AWYPQFCARVLGLTPDKTISTYPLYSNVGPALLTTNLFHGARDGLIKEGDLVLVYTIGS
                     VSTAGASVMRWGQVGLGPLP"
     misc_feature    3809..3827
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 21.8"
                     /tool="antismash"
     gene            4240..4416
                     /locus_tag="BM417_RS48405"
     CDS             4240..4416
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM417_RS48405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_177321003.1"
                     /transl_table=11
                     /translation="MSGTPIYDALMWQFSAGSRTSALWHVQGRRGVEMDDNVVLESFAG
                     TVDLAETVGDSCP"
     gene            complement(5001..6260)
                     /locus_tag="BM417_RS46420"
                     /old_locus_tag="SAMN04488564_13010"
     CDS             complement(5001..6260)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016970.5"
                     /locus_tag="BM417_RS46420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488564_13010"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_093606515.1"
                     /sec_met_domain="DUF692 (E-value: 7.6e-14, bitscore: 41.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPTLTLEIASPGAIPVALRALNLAGMKPNLVEVFWDGFCHLEPI
                     EVMGHLEEFDAAISLHISNSRFLETPEDELSGYLSRLRKSISVLAPTAVSDHVYRFTVG
                     QLRPCFPLECDYRAVERSAVDRIIRYQDTIGQTLLLENYASTSAIGGAQPEFFEEIMSK
                     TGCRLLWDVTNALVAHFNGWCEIDRWTHLLEKSRSLHVHVGTLGVDKYEGDYRVTQGDG
                     PDSASMELLKDTVRLFPVSHIVYEGSAGASAEDLAQSLLAIESAALRSPFGSAGSNPYS
                     GGGPRNRGRSGDEPQDDSRQIERALRDLYLTSTIPNDLPEGHSLWQAPLYGTPADASTA
                     EIESRMMRAGELWLENHDLSHGRLLVLSRSDAELSFGFERAASPSESAKARGPIRRRLL
                     SPLRSHGVGGYLFAETDHRL"
     gene            complement(6905..8140)
                     /locus_tag="BM417_RS46425"
                     /old_locus_tag="SAMN04488564_13012"
     CDS             complement(6905..8140)
                     /EC_number="1.14.-.-"
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.5"
                     /locus_tag="BM417_RS46425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488564_13012"
                     /product="cytochrome P450"
                     /protein_id="WP_093606516.1"
                     /sec_met_domain="p450 (E-value: 4.8e-23, bitscore: 71.7,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTPIPIHLRRFGFRLDPELERLREQEPMSRLTLPNGYRVWLVTN
                     HRLVREVLANPETFGNNEQGWFDPANEGWAPTPAAGPDREYELHGNITEYNAPEHGRLR
                     KLLAPAFSARRVKELRPLVEGIVDDCLESMISAGSPADLVKHFTLPIPSLTICELLGVP
                     YRDRVEFERRGRDRLDTKLPRAVRLRALHESRQYMVDFIASERAQPGDGFIGRLVQEHG
                     HEIDDHELVGAADILLLGGFETTAHMLALGTLLLLQTPHHWADVRNGENVDEIVEELLR
                     HLSVVQTGMPRVAHRDVEIAGQLVRAGERILCSLPSANHDPEAFAQNADVFQPLRTNKS
                     HLAFGHGIHYCLGASLARLEMRSSYQALARRLPSLRLAESPSSDQYLQSSNVFGLGSLM
                     VEWEPEDRLRAS"
     gene            complement(8168..9124)
                     /locus_tag="BM417_RS46430"
                     /old_locus_tag="SAMN04488564_13013"
     CDS             complement(8168..9124)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021599.5"
                     /locus_tag="BM417_RS46430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488564_13013"
                     /product="DUF2332 domain-containing protein"
                     /protein_id="WP_093606517.1"
                     /transl_table=11
                     /translation="MITELNLHMVRRLAEELAERTPTSSRMLFKIVGEIEDDTELGRLM
                     NNHPAANETLFPVRLLAGIQYLLVTGQTPALARHLKGLLRYGNDDEWCDQTWTLFRAAI
                     DHNPRSITAALDRPVQQHQPDRSAPLLRGLTMLGAPKVRLLELGACAGLNLIPDHYRWV
                     APTWEWGSLKSSVRLAAGGPSPGDITIVERAGCDRAPRDPRNPEDVAILRSFIPCELSV
                     ARMELDNAIELAAQVGTRVEQADAVEWLGRQLQSPTATDVYTVVWHSLFWGYLSPEEQS
                     AIEELLCRAATRMPLASICYEPAELARPARLQLRLYS"
     gene            complement(9193..9954)
                     /locus_tag="BM417_RS46435"
                     /old_locus_tag="SAMN04488564_13014"
     CDS             complement(9193..9954)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065: ABC-2 type
                     transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013245.5"
                     /locus_tag="BM417_RS46435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488564_13014"
                     /product="ABC transporter permease"
                     /protein_id="WP_093606518.1"
                     /transl_table=11
                     /translation="MILRDTWLIYTNELMQRKRQILWLVLGLFQPLLYLFAYGPLVTKL
                     MSSFWHTNTWDTFLPALLLQAALTQSMFAGLTLLWEKRMGVVERFRVAPISRYALVFGK
                     LGAIGTTTALLSFFVAMICRSFFALNSNMVNIAVCVLFNVLLSMFVAACSYALALRVNN
                     EETLSPALNALLLPLLLLSGAFIPITKDSSPGWLQQVSLFNPITYIMDADRAILRGNYF
                     DSNAVIGTAVLLGLGFFSITWVARAFTHESA"
     gene            complement(9941..10894)
                     /locus_tag="BM417_RS46440"
                     /old_locus_tag="SAMN04488564_13015"
     CDS             complement(9941..10894)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062048.1"
                     /locus_tag="BM417_RS46440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488564_13015"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_093606523.1"
                     /transl_table=11
                     /translation="MIEADGIARTYVRRGKKVEAVRDLTLRVDEGEIVGFLGPNGAGKT
                     TTMRILTTLLRPTSGKATIAGRDLIKDPNGVRARIGYVPQGTGLYDDNTALENLVLQGR
                     MHGMTKSKARQRAMDLVEQLQLTGFQGQLAKTLSGGQRRRVDIALALVHRPHLLFLDEP
                     TNGLDPQSRANLWDHIRSLRGQTTVFLSTHYLNEADALADRVFVIDHGSIVTQGSPAQL
                     KATISEGDLIQFGVPQDAVDEAARIAGGLRDASGVEASSNGLITFRSPNGEAATSHLIR
                     HLVDAEITLLSLQLTRPTLDDVFLSVTGRSLRDDSP"
     misc_feature    complement(10857..10870)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.03"
                     /tool="antismash"
     gene            complement(11045..11170)
                     /locus_tag="BM417_RS50060"
                     /old_locus_tag="SAMN04488564_13016"
     CDS             complement(11045..11170)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM417_RS50060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04488564_13016"
                     /product="hypothetical protein"
                     /protein_id="WP_281251421.1"
                     /transl_table=11
                     /translation="MNTELLTEVDALLDGADLAPADTPDMLPVFEGGGGGNGGGC"
ORIGIN
        1 tgaacccggc gttgcgcgcc ggagcgaccc tggtgacggt gtcgacgccg ttctccggcg
       61 cgaacctgct cgacgtcatc gcccgacgcc gggtcacggt cgcatacgcg gtaccgagcg
      121 tgctggccga actggcgctg caccccgacg tggaacggta cgacaccagc gctctccgac
      181 tggtcttctc cggtggagcg ccgctgccgc tcgatgtcgc caaaacgtgc gccgagcgcc
      241 tgggcgtccc cgtcgtccag ggatacggca tgaccgaggc gggctgtacg tgtgccccgc
      301 ccgacgggga acccatcacg ccgggctcga tcggccgacc gctgccggac accgagaccc
      361 gattcatcaa cccggatacc ggtgcggagg gtgaatccgg cgagatctgg gtccgcggtc
      421 ctcaggtgtc gcctggctat ctggtcgagc cgggtgtagt cgtcccgttc accgacgccg
      481 agggctggct ggacaccggt gacctcgggc gccgtgacga gcgcggctac gtgacgatca
      541 ccgggcggcg caagcaagtg atcaagtaca agggctacca ggttgctccg tccgagttgg
      601 aagaagtgct gctcacccac ccgaacgtcg tggatgctgt cgtggtcggc gtgccggacg
      661 acctcgcggg cgagctaccg aaggcgttcg tcgtgctcaa ccgtccggtc gcgctggccg
      721 gcgtggcggc gtatgtggcg gcgaaagtgg ccgcgcacaa gaagatccga ttgatcgagg
      781 cagtggaccg gattccgcgg tccgccatgg gcaaggtgcc gcgcgacgcg ttcgacggcg
      841 gcggtcgatg accatcgccg ccgcaccgcg caccaccctc gccgagcagg tgcccgaact
      901 gctcggtctg ctcggtgtca ccgcgggttt cggaatcccc ggcggcactg tggccccgac
      961 gtgggatgcc atcgcgcgaa gtcccctcga gctcctgcac ttccggcacg agtgcggtgc
     1021 ggtgttcgcc gccgtcgagg cctacttcgc cacgggtgcg ccggtcgcgg tgtgcgtcac
     1081 tgggggacct ggtatcacca acgcgatcac cggccttcac gccgcccgtt cggacggtgc
     1141 gaaggtcctg ctcgtctccg gcgcgacgcc gaagtggtcg tggggacgtc acccgttcca
     1201 ggaaacgagc ccggcggcgc tcggtgcgga catgttcacc gagggaccgc tgttccacta
     1261 cgccgcgctg gtcgacaacg agctcactga tgtcgtcgcg ccactggccg cggggatgcg
     1321 cggcccgggc ggattcgtcg cgcacctcag cctcacccgc gacgggcagt tgtcgggtgc
     1381 cacccgaccg ctgcggttgc cgccggattc cccgcaggcc ccacccgatc cagccatggt
     1441 cgcgctggtc gcggatttcc tgcgcgccga gcacatgacg ctctggcttg gcttcggcgc
     1501 gcgccacgcg gccagtgaaa tccgggagct cgtcgaacgg actggcacgc gggtcctgtg
     1561 ctcaccacga gcgaagggca tcttccctga gcgccacccg gcattcgtcg gcgtgaccgg
     1621 gctcgcaggt ggcgcgttca ccgaccaccc gcaccggatg ttggtgctgg gcagcggttt
     1681 gggggagatg acctccttct ggagccctga cttggtccca ccaaggggtt ttgtccacgt
     1741 cgacctcgac gaggacgtgc ctggctccgc gtatccgggc gtgccgctga tccccgttca
     1801 ctgcgaaatc agggctttcc tgcgcgaggt gctcgacgca ctacccgctc ggacggccga
     1861 accgccgccg ggtcgacggg ctcggccggt ggaagctccg cgctccgggc cagtgcggcc
     1921 tcaagtgctg atggccgcgg tgcagcgcgt gatcgtcgac ggtagcgacg cgacagtcct
     1981 cgcggaagcc ggcaactcct tcctgtgggc ggggcgacac ctcgagttcg acacagcccg
     2041 ataccgggtg agcaccggtt tcggggcaat gggccacgca gcaacaggtg tgctgggagc
     2101 cgctcacgcg gtggacggca aggcggtcgc aattgtcggc gacggcgcgc tgttgatgaa
     2161 caacgagatc agcacagctg tgcagtacca gatcccagcg gtctgggtgg tgctcaacga
     2221 cagcgcctac ggcatggtgc ggcagggcat gacagccgaa gggctccggc ccgagggggt
     2281 ccggatgccg cgcaccgact tcgtgggatt ggcccgtgcc atgggagccg acggcatcac
     2341 ggtgaccgac gagaccgaac tggaccaggc gttggcggtg gctttggccg cgccagggcc
     2401 ttttgtgctc gacgtgttgg tggacgacac gtgctcggcg ccgatgggcg aacgcgtcgc
     2461 cagcctgacc tctcaacgaa tggagctgtg atcatgcctg gtggtgtcgg aattcggtcg
     2521 ttggccgtgg cgtttccggc ctcggtacgg accaacgact acttccggga gaactacccg
     2581 gagctggtgg ccactgcgga gtcccggtcg ttggcccagg tgttctcgcc ccaggacgag
     2641 aaggtacggc tcgacacgtg ggaacgcgag atggcgccct accttgccga tccgttccgg
     2701 ggggcggtcg aacgccggat ccttgcaccc ggcgaaaccg cgctggacct ggaagcacgc
     2761 gcggcccggc gggcgctgga cgccgcaggc ctggacgtcg cggatgtcga cctcgccatc
     2821 gtgacctcgt tcatgcccga ccagctaggc cccggaaacg ccgcgtacct cgcccgtgag
     2881 ctcgggatgc gaggcaccgc gtggaacgtc gagagcgcct gctcaagcgg tcttgtgtcg
     2941 ctggagaccg ccaccgccct ggtggaggca ggcaggtacg aaatcgtgct ggtggtcatt
     3001 tcctgcacct actcgcgggt ctcggactac tcggacacgc tcgcctggtt ctggggtgat
     3061 ggcgcgggtg ccttcgtcgt gggccgtaca ccggaaggcg tgggcgtcat gggctcgatg
     3121 agcaccaaca ccgcgtcgac ctgcgatacg ttctccaacc acatcgtggt cgatgagcac
     3181 ggcgaaccgc acatccgaac caccgcttcg aaggccacgg gacgcattct ccgcgatacg
     3241 gccgaggcct cactgcgcga gacttgcgag ggcgcgctcg cgtgcgccgg agtggacctg
     3301 ctggacatcg acttcttcgt gtgcaactcg ccggtcgcgt ggtacccgca gttctgcgcc
     3361 cgcgtgctcg gcctcacgcc ggacaagacc atcagcacgt atccgttgta cagcaacgtc
     3421 ggcccggcgc tgctcaccac caacctcttc cacggagctc gtgacggact gatcaaggag
     3481 ggcgacctcg tgctcgtcta cacgatcggc tccgtgtcca cggcgggcgc gtcggtcatg
     3541 cgctggggcc aggtcggtct ggggccgctg ccgtgagaat ccgccgattg tccgatcggg
     3601 gaacatactc ggtgtacagc cgcagcacgg cccgccgagc ctggggaaga tcacaggtcg
     3661 ccgagctgga gaaggacaag gcgttcttgg tgaaagcttc ggtgtgcctt gcagcgattg
     3721 gaggagaacc ggtctggcgc atggcgcgac tgctggagat ctcgacctcc ggctgctacg
     3781 cacacttgaa gcgttgtgct gcaacggtgt tgagaccgac ctgcaacgtc gcgcgaacgg
     3841 accgctcgcg ctcgtgtgag caatgtgctt gccaagttcg tgccgccctc ttgtgtgtgg
     3901 ccgtacgtgg aggccctgtg agtggtcctt gccaggcagc tggatgacta cttccggtgg
     3961 cagaaggtca acgacgggga actccgaggg tgtcgagcag ttcacggaag acgtcgtctg
     4021 gcggtttgcc gatgccgccg gccgcgaagc cgccaaggtc gacgttgact ctgttcgtcc
     4081 ggataggacg gtgccacccg gcggatctgg tggggccatg cgaagtcgaa gggcacgacg
     4141 cgccgcagtt aaggaaaccg tgatgtgctg atcacccgat gcggggattg cgaggggttc
     4201 tgtcgttgtg ggagctacgt tcggggagaa gattccgctg tgagtgggac accgatctat
     4261 gacgcgctca tgtggcagtt ctcggccggt tctcggacgt cggcgctgtg gcacgtccag
     4321 gggcgacgtg gcgtcgagat ggacgacaac gtcgtgctgg agtcgttcgc cggcacggtc
     4381 gacttggctg agaccgtagg agattcctgc ccgtgagcaa gcacggccga gagctcgtcg
     4441 aggcctgtct gtgccggtac ctcagcctgg ttgccgcggt gctcgacgtt ccgctcggcc
     4501 tggttgcctt gacgtgcgga caacacggcg atgggatgag ctatctggct gggccgtcgt
     4561 tgtccagagc gatgatcaac ctgacatcgt tgtgcggcag ggaacagatg ttgttccacc
     4621 acctcgtgtt gtcggtgtgt tcacgctgtt gctcgtagcc tacgccgaat gaggtgccgc
     4681 gctggcggtc gcacgactga ggccgcacca ctgacgacat ggtcgtcatc accacgcttc
     4741 aggccgaagg cgactatctt gaagatgcca agtgatcagg cgcgttcgga tacacgatgc
     4801 tggcgcacta ttgaccattg gaacttcgag tcgttgctct cgaagggttt gtcggctccc
     4861 ggcggtgtgt actcatgaag gcgaggcctc ggttgctgct cattcgtcgt gcgtcggcgg
     4921 aagggagcag gggtcggccc ggtgggctcg tcgaaacttt ggctggtcac ctcgcgagga
     4981 cggcatcgcg ggaagggtct tcacagtcgg tggtcggtct ccgcgaagag atatccgcca
     5041 acgccgtgcg accgcagtgg cgaaagcaat cttcgccgaa tcggcccgcg agccttggct
     5101 gactcactgg gtgacgccgc tctctcgaat ccgaacgaca gctccgcgtc cgaacgagaa
     5161 agcaccagga gacggccgtg cgagagatcg tggttttcca gccacagttc acctgctcgc
     5221 atcattcgtg actcgatctc ggccgtactg gcgtccgccg gagttccgta caacggcgcc
     5281 tgccaaagcg agtgaccctc cggcaggtcg ttcgggatcg tggaagtgag gtacagatct
     5341 cgaagtgcgc gctcgatctg cctgctgtca tcctggggtt cgtcaccact ccgcccccgg
     5401 tttctcggcc ctcctccgga gtacggattg cttcccgccg aaccgaacgg gctgcgcagc
     5461 gcggcgctct cgatggcgag caggctctgg gcaagatctt ccgccgaggc ccccgcggag
     5521 ccctcataga cgatgtgact caccggaaac agccgcacag tgtccttcag cagctccatg
     5581 gaagccgagt ccggcccgtc gccttgggta actcggtagt cgccttcgta cttgtcgacg
     5641 ccgagggtgc ccacgtgcac atgcaatgat cgacttttct ccaaaagatg ggtccagcga
     5701 tcgatctcgc accagccgtt aaagtgcgcc acgagtgcat tggtgacatc ccagagaagg
     5761 cgacatccgg tcttcgacat tatttcctca aagaattccg gctgtgctcc gccaattgcg
     5821 gaagtcgaag cgtagttctc cagaaggaga gtctggccaa tcgtgtcctg gtaacgaatg
     5881 attcgatcta ctgcacttct ctcgactgcg cgatagtcgc actccagtgg aaagcaggga
     5941 cgcagctgcc cgacggtgaa tcggtaaacg tgatccgaaa cagctgtggg agcgagaacc
     6001 gaaatagact tccgcaggcg cgacaaatag cccgacagtt cgtcttcagg agtctccaga
     6061 aagcgcgaat tcgatatgtg caacgatatg gcagcgtcga actcctcgag gtgtcccatg
     6121 acctcaatcg gttccaaatg acagaagcca tcccagaaca cttcaacgag gttgggcttc
     6181 attccagcca gattcaacgc acgcaacgcc accgggatcg cacctggact ggcgatctcc
     6241 aaagtcagcg taggaatcat cgcacacctc gacggacgtc agacctggga tacttcgcgc
     6301 ggatgccctt gttctcgcgc acggcgacca ccctggccgg cggtcatgtc gccggggaga
     6361 tcgggcgccg ctgccggact cgacctcccc ggcacgtatc attcggttgc ttcttcctcc
     6421 gtcttctcgc attcgccgtt ccagcagtac ctcatggggg agacggagac gggaactgct
     6481 tccttgagca gttccagcgc ttcatcgaac atggctcacc tcatcactga tgggttcctg
     6541 tcgaaggcga tcgtcccgat ttctgcaatc gattggaaca atcgatcggt attgtaggtt
     6601 taagcggaaa gctaagtcaa gaccgcttct cggaagtggt gacgtctgaa aataggtacg
     6661 acaattatgc cctggtcccc ggcggggcca aggcttgcaa ctcggcgcct tgtgcagcgt
     6721 tccgtgcagg ttccacctcg tgaggagtag ggttttggtg cccgcaaatg aaccccaaag
     6781 agaagaagaa gtgcattgaa gattgcactt tcttgcattg tgtcagagta gtgcggccgc
     6841 tcctcgatca tgaacaggtc gtcgttcggt gttgtggaca actgccggca cgttgcggcg
     6901 cttgtcagct cgcgcgtagt cgatcttcag gctcccactc caccatcaac gagccgaggc
     6961 cgaaaacatt ggaggactga aggtactgat cactcgaagg cgactcggcc aagcggaggc
     7021 tgggaagtcg tcgggcaaga gcctgatacg aggatcgcat ttccagccgg gcgagcgaag
     7081 cccccaggca gtagtggatc ccgtgaccga aggcgagatg ggacttgttc gtgcggagcg
     7141 gttgaaagac gtcggcgttt tgagcgaacg cttcgggatc gtggttggcg gatggaagag
     7201 agcacaggat ccgctcgccg gcgcgtacca gttgtcccgc gatctctacg tcacgatggg
     7261 caactctggg cataccggtt tggacgaccg ataggtggcg aagtagctcc tcgacgattt
     7321 cgtcaacatt ctcgccgttg cgtacgtccg cccagtggtg aggggtctgc agcaagagca
     7381 gagtgccgag ggccagcatg tgtgcggtcg tctcgaagcc gccgagcaag aggatgtccg
     7441 ctgcacccac gagttcgtgg tcgtctattt cgtgaccgtg ctcctggacg agtctgccaa
     7501 tgaatccatc gccaggttgg gcgcgctcac tcgcgatgaa gtccaccatg tactgcctgg
     7561 attcgtgaag tgctcgcaat ctgaccgcac gcgggagttt cgtgtccagg cgatctcggc
     7621 cgcggcgttc gaactcgact cggtctcggt acgggacacc aaggagctcg cagatcgtca
     7681 acgacggaat ggggagcgtg aagtgtttga cgagatctgc gggacttccc gcactgatca
     7741 tcgactccaa gcagtcgtcg actatgcctt ctacgagagg acgtagttcc ttcacgcggc
     7801 gcgcgctgaa ggctggagca agcagtttgc gcagcctgcc gtgctctgga gcgttgtatt
     7861 ccgtgatgtt gccgtggagt tcgtattctc gatccggccc agctgcaggc gtcggtgccc
     7921 acccctcgtt ggcggggtcg aaccagcctt gttcgttgtt tccgaaagtc tccgggttcg
     7981 cgagcacctc gcggaccagg cgatggttcg tcacgagcca gacacgatag ccgttcggca
     8041 gtgtaagtct gctcatcggt tcttgctcgc gcaacctctc gagctcagga tcgagcctga
     8101 atccgaaccg gcggagatga atcgggatag gagtcgacat gcgcgggcct caccgttgct
     8161 gtcagggtca ggagtaaagc cggagttgca accgtgcagg ccttgccaat tcggccggct
     8221 cgtagcagat gcttgccagc ggcattcgtg tggccgcgcg gcacagcagc tcttcgatcg
     8281 cggattgttc ctccgggctc aggtaacccc agaacaagct gtgccacaca actgtgtaga
     8341 catctgtggc agtcgggctt tgcagctgtc tgccgagcca ctccaccgcg tcggcttgct
     8401 cgacacgagt gccgacctga gcggcgagtt cgatcgcgtt gtccagttcc atgcgcgcga
     8461 cgctgagttc gcacggaatg aacgatcgta ggatggcaac gtcctccggg ttgcgagggt
     8521 cgcgtggcgc cctgtcgcaa ccagccctct cgacgatggt gatgtcgccg ggtgatgggc
     8581 cgcctgcggc cagtcgaacg ctcgacttca ggcttcccca ctcccaggtc ggagcgaccc
     8641 accggtagtg gtccggaatc agattgagtc cggcgcacgc cccgagctcg agaaggcgca
     8701 ccttcggtgc tcctagcatg gtgagcccgc ggagcagggg agccgaacga tcgggctggt
     8761 gttgctggac aggacggtcg agcgccgcgg tgatggaacg tgggttgtgg tcgatggcgg
     8821 cgcggaacag agtccacgtc tgatcacacc attcgtcgtc gttgccgtac ctcaggagac
     8881 ccttcagatg acgagccagt gctggggtct ggccggtcac cagcagatac tgaatacctg
     8941 cgagcaatcg gactggaaaa agtgtctcgt ttgcggcggg gtgattgttc atgagtcgcc
     9001 ccaactccgt gtcgtcctcg atctcaccga cgatcttgaa cagcattctg gaggaggtgg
     9061 gtgtgcgctc ggccagctcc tccgccaacc gccgcaccat gtggaggttg agttcggtga
     9121 tcatttcggc actccgctct gtgagcgttc ggggaggatg ttggtccctg tggccctcgt
     9181 ctggaagcgt ttctaggcgc tttcgtgggt gaaggcccgg gcgacccaag ttatggagaa
     9241 gaagccgagg cccagcagta ccgctgttcc gatcaccgcg ttggaatcga aatagttgcc
     9301 gcgcaggatc gctcggtctg cgtccatgat gtaggtgatg gggttgaaga gggagacctg
     9361 ctgcagccat ccaggggatg aatctttagt gatcgggatg aaggcgcctg atagaagcag
     9421 tagcggtagg agcagcgcgt tcaaggctgg gctcagcgtt tcctcgttgt tgacgcgcag
     9481 tgcgagcgcg tatgagcacg cggctacaaa catggacaac aaaacgttga acaggacgca
     9541 caccgctatg ttgaccatgt tggagttgag cgcgaaaaat gatcgacaga tcatggcgac
     9601 gaagaacgac agtagagctg tggtggttcc aattgctccg agcttgccga agacgagcgc
     9661 gtaacgactt atcggcgcaa ctctgaaccg ctcgaccaca cccatgcgct tttcccagag
     9721 caaggtgagc cctgcgaaca tgctctgcgt cagggcggct tgcagtagta gagcgggcag
     9781 gaaggtgtcc cacgtattgg tgtgccagaa cgaggacatg agcttcgtga ccaggggccc
     9841 gtacgcgaag aggtagagca aaggctgaaa cagccccagg acaagccaca agatctgacg
     9901 cttgcgctgc atgagctcgt tcgtgtagat cagccaagtg tcacggagaa tcatcgcgca
     9961 agctccgtcc ggtcaccgat aggaagacgt cgtcaagagt cgggcgcgtg agttgcagcg
    10021 acaacagcgt tatttctgcg tctaccaagt gtcggatcag gtggctcgtc gcagcttctc
    10081 cgttcggaga gcggaacgtg atcaaaccat tcgagctggc ttctactcct gacgcgtcgc
    10141 gcaatccgcc agcgatgcgg gctgcttcgt cgactgcgtc ttggggcacg ccgaattgaa
    10201 tgaggtcgcc ctctgagatc gtcgccttga gttgagccgg gcttccttgt gtgacgatgc
    10261 tcccgtgatc aatgacgaac acgcggtccg cgagagcgtc tgcctcgttg aggtagtggg
    10321 tgctgaggaa caccgtcgtt tgaccgcgga gcgatcggat gtgatcccac agattagccc
    10381 tgctctgcgg gtccagtcca ttggtcggct cgtcgaggaa gagcaagtgg ggacggtgga
    10441 ccagcgcgag tgcgatgtcg acgcggcggc gctggccgcc ggacaaggtc ttcgccagct
    10501 gaccttgaaa accggtcaat tgaagttgtt caacgagatc cattgctcgt tgccgtgcct
    10561 tgctcttcgt cattccgtgc atgcgcccct gtagaaccag attttcgagc gcggtgttgt
    10621 cgtcgtaaag cccagttcct tgcgggacgt agccgatgcg ggcgcgcacg ccgttgggat
    10681 ccttgatcag atcgcggccg gcgatggtcg ctttcccgga cgtggggcga agcagagtgg
    10741 tcaggatgcg catggtggtt gtcttgccgg cgccgttggg gccgagaaag ccgacgatct
    10801 cgccttcgtc cacgcgtagt gtcaggtcgc gcacggcctc cacctttttg ccgcgtcgaa
    10861 cgtacgtccg ggctatgcca tccgcttcga tcatgaggcc caccacattg aaagttcact
    10921 ctgaatcgtc ttcatgatcc accaccatgg ggccagtcac ccggacgcca gtggacggag
    10981 cctgaagcgg gccgctcgtt ctcggaggtg gaatcctccg agaacgagcg gaaggaacga
    11041 cagctcagca gccgccgccg ttgccgccgc cgcccccttc gaacacggga agcatgtcgg
    11101 gggtatcggc gggagccagg tcggctccat cgaggagggc gtcgacttcg gtgaggagtt
    11161 cagtgttcat tcgactgctc cgatcacaaa gtgggggagg tagatcttga cgcgcaacgc
    11221 gtgaagtcgt gttcatcgcc gagatcacac atccgtctgg
//
