LOCUS       NZ_FOWL01000041         1655 bp    DNA     linear   CON 22-AUG-2024
DEFINITION  Variovorax sp. OK605, whole genome shotgun sequence.
ACCESSION   NZ_FOWL01000041
VERSION     NZ_FOWL01000041.1
KEYWORDS    WGS; RefSeq.
SOURCE      Variovorax sp. OK605
  ORGANISM  Variovorax sp. OK605
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Variovorax.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900115445.1-RS_2024_08_22
            Annotation Date                   :: 08/22/2024 03:36:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,179
            CDSs (total)                      :: 8,110
            Genes (coding)                    :: 8,025
            CDSs (with protein)               :: 8,025
            Genes (RNA)                       :: 69
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 61
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 85
            CDSs (without protein)            :: 85
            Pseudo Genes (ambiguous residues) :: 0 of 85
            Pseudo Genes (frameshifted)       :: 21 of 85
            Pseudo Genes (incomplete)         :: 74 of 85
            Pseudo Genes (internal stop)      :: 8 of 85
            Pseudo Genes (multiple problems)  :: 16 of 85
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:45:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1655
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOWL01000041.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1655
                     /db_xref="taxon:1855317"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0070142_141"
                     /organism="Variovorax sp. OK605"
                     /strain="OK605"
     gene            <1..>1655
                     /locus_tag="BM013_RS40310"
                     /old_locus_tag="SAMN05216567_1411"
     region          1..1655
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1655
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1655
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>1655](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>1655
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>1655
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (31-56). E-value:
                     1.1e-15. Score: 48.6. Matches aSDomain:
                     nrpspksdomains_BM013_RS40310_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_Dual (34-321). E-value:
                     2.9e-122. Score: 399.6. Matches aSDomain:
                     nrpspksdomains_BM013_RS40310_Condensation_Dual.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542704.1"
                     /locus_tag="BM013_RS40310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216567_1411"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_143098532.1"
                     /sec_met_domain="Condensation (E-value: 4.6e-49, bitscore:
                     153.1, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 4.8e-17, bitscore:
                     47.6, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCEAITPQMLTLVTLDAQQIATVAAGVPGGAANIQDIYPLVPMQE
                     GMLFHHLLQTEGDAYVTLNSLSFDSRERLERFVASFNEVIARHDILRTAVLWEGLPEPV
                     QVVYRHAVLQLQWLESAQQLDATVDPRHYRIDVRRAPMLGAIAAHGAATGKWLLQILSH
                     HLVDDNTTLKLIVEEIALIQQGRADALAAPVAFRHFVAQARLGVSQAEHETFFRQMLGD
                     VQEPTAPFNLLGVQGDGLEVDEARLSLETGLALQLRQQAQRHGVSAATLCHLAWALVVG
                     KATGKDDVVFGTVLFGRMQNEGAARALGLFINTLPLRVRLGAQGVAQSLRQTHAALSAL
                     LHHEHASLSLAQRCSGLPASTPLFSTLLNYRHTPQGDGASLALGLEGMAVLGDRERTNF
                     PMSMLVNDLGDAFVLIAQVSRTLDAQRICGLMEAALRGVVEALARQPEQAVSEVELLGE
                     AERAQLRRWSVNGQRHAGEQPVHRLIEQQVRERPDAPALAFGEQSLSYGELNARANRLA
                     HRLIAMGVKPESKVGIAAERSVEMVVGILAILKAG"
     aSDomain        94..168
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM013_RS40310_NRPS-COM_Nterm.1"
                     /evalue="1.10E-15"
                     /label="BM013_RS40310_NRPS-COM_Nterm.1"
                     /locus_tag="BM013_RS40310"
                     /protein_end="56"
                     /protein_start="31"
                     /score="48.6"
                     /tool="antismash"
                     /translation="ANIQDIYPLVPMQEGMLFHHLLQTE"
     aSDomain        103..963
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM013_RS40310_Condensation_Dual.
                     1"
                     /domain_subtypes="Condensation_Dual"
                     /evalue="2.90E-122"
                     /label="BM013_RS40310_Condensation_Dual.1"
                     /locus_tag="BM013_RS40310"
                     /protein_end="321"
                     /protein_start="34"
                     /score="399.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHLVDDN"
                     /tool="antismash"
                     /translation="QDIYPLVPMQEGMLFHHLLQTEGDAYVTLNSLSFDSRERLERFVA
                     SFNEVIARHDILRTAVLWEGLPEPVQVVYRHAVLQLQWLESAQQLDATVDPRHYRIDVR
                     RAPMLGAIAAHGAATGKWLLQILSHHLVDDNTTLKLIVEEIALIQQGRADALAAPVAFR
                     HFVAQARLGVSQAEHETFFRQMLGDVQEPTAPFNLLGVQGDGLEVDEARLSLETGLALQ
                     LRQQAQRHGVSAATLCHLAWALVVGKATGKDDVVFGTVLFGRMQNEGAARALGLFINTL
                     PLRVRL"
     CDS_motif       112..153
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM013_RS40310_0001"
                     /evalue="8.50E-08"
                     /label="C1_dual_004-017"
                     /locus_tag="BM013_RS40310"
                     /protein_end="51"
                     /protein_start="37"
                     /score="24.2"
                     /tool="antismash"
                     /translation="YPLVPMQEGMLFHH"
     CDS_motif       190..291
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM013_RS40310_0002"
                     /evalue="1.00E-14"
                     /label="C2_dual_024-063"
                     /locus_tag="BM013_RS40310"
                     /protein_end="97"
                     /protein_start="63"
                     /score="47.0"
                     /tool="antismash"
                     /translation="NSLSFDSRERLERFVASFNEVIARHDILRTAVLW"
     CDS_motif       487..546
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM013_RS40310_0003"
                     /evalue="2.30E-04"
                     /label="C3_dual_135-156"
                     /locus_tag="BM013_RS40310"
                     /protein_end="182"
                     /protein_start="162"
                     /score="14.2"
                     /tool="antismash"
                     /translation="HHLVDDNTTLKLIVEEIALI"
     CDS_motif       586..624
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM013_RS40310_0004"
                     /evalue="4.20E-05"
                     /label="C4_dual_169-182"
                     /locus_tag="BM013_RS40310"
                     /protein_end="208"
                     /protein_start="195"
                     /score="16.3"
                     /tool="antismash"
                     /translation="FRHFVAQARLGVS"
     CDS_motif       862..954
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM013_RS40310_0005"
                     /evalue="1.70E-15"
                     /label="C5_dual_260-291"
                     /locus_tag="BM013_RS40310"
                     /protein_end="318"
                     /protein_start="287"
                     /score="49.1"
                     /tool="antismash"
                     /translation="DVVFGTVLFGRMQNEGAARALGLFINTLPLR"
     CDS_motif       1009..1077
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM013_RS40310_0006"
                     /evalue="3.30E-09"
                     /label="C67_dual_14fromHMM"
                     /locus_tag="BM013_RS40310"
                     /protein_end="359"
                     /protein_start="336"
                     /score="29.2"
                     /tool="antismash"
                     /translation="LSALLHHEHASLSLAQRCSGLPA"
ORIGIN
        1 ggctgcgagg cgatcacgcc gcagatgctc acgctggtga cgctggacgc gcagcagatc
       61 gcaaccgtcg cagccggagt gccgggcggc gcggccaaca tccaggacat ctacccgctg
      121 gtgccgatgc aggaaggcat gctgttccat cacctgctgc agaccgaggg cgatgcgtac
      181 gtgacgctga acagcctgag cttcgacagc cgggagcggc tggagcggtt cgtcgcgagc
      241 ttcaacgagg tgatcgcacg gcacgacatc ctgcgcacgg cggtgctgtg ggaaggtctg
      301 cccgagccgg tgcaggtggt gtaccggcat gccgtgctgc agctgcagtg gctggagagt
      361 gcgcagcagc tggacgccac ggtcgacccg aggcactacc gcatcgacgt gcgccgcgcg
      421 ccgatgctcg gtgccatcgc cgcccacggc gcggccaccg gcaaatggct gctgcagatc
      481 ctcagccacc acctggtgga cgacaacacg acgctgaagc tgatcgtcga ggagatcgcg
      541 ctgatccagc agggccgcgc ggacgcattg gcggcaccgg tggcgttcag gcacttcgtg
      601 gcgcaggcgc gcctgggcgt gagccaggcg gagcacgaga cgttcttccg ccagatgctg
      661 ggtgatgtgc aggaacccac ggcacccttc aacctgctgg gcgtgcaggg cgacgggctc
      721 gaggtcgacg aggcacgcct gtcgctggag acgggccttg ccttgcagct gcgacagcag
      781 gcgcagcgcc atggcgtgag cgcggcgacg ctgtgccacc tggcctgggc gctggtggtg
      841 ggcaaggcca cgggcaagga cgacgtggtg ttcggcaccg tgctgttcgg ccgcatgcag
      901 aacgaaggcg ccgcccgcgc gctgggtctt ttcatcaaca cgctgccgtt gcgcgtgcgg
      961 ctgggcgccc aaggcgtggc gcagagcctg cgccagacgc atgcggcgct cagcgccttg
     1021 ctgcatcacg agcatgcgag cctgtcgctt gcgcaacgtt gcagcgggct gccggcgagc
     1081 acgccgctgt tctccacgct gctcaactac cgccacacgc cgcagggcga tggtgcgtcg
     1141 ctggcgttgg gactcgaagg catggcggtg ctgggagacc gggagcgcac caacttcccg
     1201 atgtcgatgc tggtcaatga cctgggcgat gccttcgtgc tgatcgcgca ggtcagccgg
     1261 acgctcgatg cgcagcgcat ctgcggcctg atggaagcgg cactccgggg tgtcgtcgag
     1321 gctttggcca ggcagcctga gcaggcggtc agcgaggtgg agcttctggg tgaggccgag
     1381 cgcgcacagc tgcggcggtg gagcgtgaac gggcagcgcc atgcgggtga gcagccggtg
     1441 caccggctga tcgagcagca ggtgcgtgaa cgccccgacg ctcccgcgct ggcgttcggc
     1501 gagcagtccc tgagctacgg cgaactcaac gcacgggcca accgcctcgc gcaccgactg
     1561 atcgcaatgg gcgtaaagcc cgagagcaag gtgggcatcg cggcagaacg ttccgtcgag
     1621 atggtggtgg gcatcctcgc gatcctcaag gccgg
//
