LOCUS       NZ_FOSG01000046         3042 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces pini strain PL19, whole genome shotgun sequence.
ACCESSION   NZ_FOSG01000046
VERSION     NZ_FOSG01000046.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces pini
  ORGANISM  Streptomyces pini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900114215.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:14:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,762
            CDSs (total)                      :: 5,677
            Genes (coding)                    :: 5,560
            CDSs (with protein)               :: 5,560
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 20 of 117
            Pseudo Genes (incomplete)         :: 111 of 117
            Pseudo Genes (internal stop)      :: 1 of 117
            Pseudo Genes (multiple problems)  :: 15 of 117
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3042
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOSG01000046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     aSDomain        1..972
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28540_AMP-binding.1"
                     /evalue="2.10E-81"
                     /label="BM237_RS28540_AMP-binding.1"
                     /locus_tag="BM237_RS28540"
                     /protein_end="324"
                     /protein_start="0"
                     /score="265.5"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="MPLDPDLPVERLRFMVAEAGVGCVVADAATAGTAAGLDAAVVRAD
                     TAGFDPDGPRRTPDPHDLAYTIFTSGSTGRPKAVGVEHHALAAHVAAARDRYGITAEDR
                     VLAFASFSFDASLDQVLPALTCGAQVIVRPDEPWLPTQVPEVVERHGVTVVNLPPTYWN
                     ELAFSLDRRAAARLAGLRLLILGGESVPAGALAAWRAEAPHVRVCNAYGPTETTVTSTT
                     YEVTGPVEGRVPIGRPLGSRRVYVVDAHDRPVDVGVPGELLVGGPELARGYLGRPALTA
                     ERFVPDPFASGGGRLYRTGDIVRWLPSGDLEFLGRSDDQVKIR"
     aSModule        1..1533
                     /complete
                     /domains="nrpspksdomains_BM237_RS28540_AMP-binding.1"
                     /domains="nrpspksdomains_BM237_RS28540_PCP.1"
                     /locus_tags="BM237_RS28540"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     source          1..3042
                     /db_xref="taxon:1520580"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0075229_146"
                     /organism="Streptomyces pini"
                     /strain="PL19"
                     /type_material="type strain of Streptomyces pini"
     gene            <1..>3042
                     /locus_tag="BM237_RS28540"
                     /old_locus_tag="SAMN05192584_1461"
     region          1..3042
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..3042
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3042
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>3042](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>3042
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>3042
                     /NRPS_PKS="Domain: AMP-binding (0-324). E-value: 2.1e-81.
                     Score: 265.5. Matches aSDomain:
                     nrpspksdomains_BM237_RS28540_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (444-511). E-value: 1.6e-25. Score:
                     81.1. Matches aSDomain: nrpspksdomains_BM237_RS28540_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (528-828). E-value:
                     2.5e-102. Score: 333.8. Matches aSDomain:
                     nrpspksdomains_BM237_RS28540_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016827425.1"
                     /locus_tag="BM237_RS28540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_1461"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_139238207.1"
                     /sec_met_domain="AMP-binding (E-value: 5e-99, bitscore:
                     317.7, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.4e-15, bitscore:
                     43.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 5.2e-68, bitscore:
                     215.4, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLDPDLPVERLRFMVAEAGVGCVVADAATAGTAAGLDAAVVRAD
                     TAGFDPDGPRRTPDPHDLAYTIFTSGSTGRPKAVGVEHHALAAHVAAARDRYGITAEDR
                     VLAFASFSFDASLDQVLPALTCGAQVIVRPDEPWLPTQVPEVVERHGVTVVNLPPTYWN
                     ELAFSLDRRAAARLAGLRLLILGGESVPAGALAAWRAEAPHVRVCNAYGPTETTVTSTT
                     YEVTGPVEGRVPIGRPLGSRRVYVVDAHDRPVDVGVPGELLVGGPELARGYLGRPALTA
                     ERFVPDPFASGGGRLYRTGDIVRWLPSGDLEFLGRSDDQVKIRGFRVELGEVEAVLREC
                     PRVAAAAVRPDTGTGQTLIGYVVTGPDGPDGTGGTDGTGGPVEPAALRAWCAERLPHYA
                     VPSDVVLLDALPVNASGKLDRAALPDARPDRATGRVAHTAPRDESERIIASVWADVLGV
                     ERVGIDDGFFDLGGHSLLATMAVSRVAERLGRDIELRTLFENPRIREFGPAVAAARRAA
                     GTAVTPVDRSGPLPMSFAQERLWFLDRTSEAGDEYVLWFSWRLRGGLDRDAWQAALDDM
                     TARHEVLRTALVEVDGRPAQQVCDPMGVPLEWRQAPGGEDEAARLEAVREQAHALATRR
                     FDLDRPPMLRAGVWELGPRDHVAVLAFHHVATDGWSKDVFTGELAACYRARLAGRAPAL
                     PEPPVQYGDFAVWQRDRAGDGTLEDRLDYWEEELRDLPVLRLPTDRPRPAAWSGRGGSV
                     EVGLPAELASRLDAFAREQGATRFMVLLTAVQTVLARWSGQDDVPVGTPVAGRGRTELE
                     RLVGFFVNTVVLRGDLSGSPTFTELLARVRATVLGAFDHQDVPFEKLVERLRPERDLSR
                     NPLFQVMVDVQESPTSGPDIEGLEATGFDLPWASAKFDLTTTFLLYPDRFALNVEYAAD
                     LFDAATATRLAEHVGRVLAAVTADPDIPVHRVDLLSPAEHAHLVATAGTDRLAAGSVPV
                     VAVGGEPGASALVCGERSVTYGELE"
     CDS_motif       190..249
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0001"
                     /evalue="1.90E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="BM237_RS28540"
                     /protein_end="83"
                     /protein_start="63"
                     /score="26.5"
                     /tool="antismash"
                     /translation="AYTIFTSGSTGRPKAVGVEH"
     CDS_motif       631..660
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0002"
                     /evalue="8.60E-03"
                     /label="NRPS-A_a5"
                     /locus_tag="BM237_RS28540"
                     /protein_end="220"
                     /protein_start="210"
                     /score="8.9"
                     /tool="antismash"
                     /translation="YGPTETTVTS"
     CDS_motif       766..852
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0003"
                     /evalue="1.40E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="BM237_RS28540"
                     /protein_end="284"
                     /protein_start="255"
                     /score="49.3"
                     /tool="antismash"
                     /translation="VGVPGELLVGGPELARGYLGRPALTAERF"
     CDS_motif       937..1002
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0004"
                     /evalue="1.50E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="BM237_RS28540"
                     /protein_end="334"
                     /protein_start="312"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FLGRSDDQVKIRGFRVELGEVE"
     aSDomain        1333..1533
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28540_PCP.1"
                     /evalue="1.60E-25"
                     /label="BM237_RS28540_PCP.1"
                     /locus_tag="BM237_RS28540"
                     /protein_end="511"
                     /protein_start="444"
                     /score="81.1"
                     /tool="antismash"
                     /translation="ERIIASVWADVLGVERVGIDDGFFDLGGHSLLATMAVSRVAERLG
                     RDIELRTLFENPRIREFGPAVA"
     aSDomain        1585..2484
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28540_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.50E-102"
                     /label="BM237_RS28540_Condensation_LCL.1"
                     /locus_tag="BM237_RS28540"
                     /protein_end="828"
                     /protein_start="528"
                     /score="333.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHVATDG"
                     /tool="antismash"
                     /translation="PLPMSFAQERLWFLDRTSEAGDEYVLWFSWRLRGGLDRDAWQAAL
                     DDMTARHEVLRTALVEVDGRPAQQVCDPMGVPLEWRQAPGGEDEAARLEAVREQAHALA
                     TRRFDLDRPPMLRAGVWELGPRDHVAVLAFHHVATDGWSKDVFTGELAACYRARLAGRA
                     PALPEPPVQYGDFAVWQRDRAGDGTLEDRLDYWEEELRDLPVLRLPTDRPRPAAWSGRG
                     GSVEVGLPAELASRLDAFAREQGATRFMVLLTAVQTVLARWSGQDDVPVGTPVAGRGRT
                     ELERLVGFFVNTVVLRGDL"
     CDS_motif       1597..1629
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0005"
                     /evalue="2.80E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="BM237_RS28540"
                     /protein_end="543"
                     /protein_start="532"
                     /score="10.2"
                     /tool="antismash"
                     /translation="SFAQERLWFLD"
     CDS_motif       1666..1758
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0006"
                     /evalue="7.30E-13"
                     /label="C2_LCL_024-062"
                     /locus_tag="BM237_RS28540"
                     /protein_end="586"
                     /protein_start="555"
                     /score="40.9"
                     /tool="antismash"
                     /translation="FSWRLRGGLDRDAWQAALDDMTARHEVLRTA"
     CDS_motif       1984..2049
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0007"
                     /evalue="1.10E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="BM237_RS28540"
                     /protein_end="683"
                     /protein_start="661"
                     /score="27.5"
                     /tool="antismash"
                     /translation="FHHVATDGWSKDVFTGELAACY"
     CDS_motif       2098..2133
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0008"
                     /evalue="3.20E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="BM237_RS28540"
                     /protein_end="711"
                     /protein_start="699"
                     /score="16.5"
                     /tool="antismash"
                     /translation="QYGDFAVWQRDR"
     CDS_motif       2323..2376
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0009"
                     /evalue="1.30E-03"
                     /label="Cy4"
                     /locus_tag="BM237_RS28540"
                     /protein_end="792"
                     /protein_start="774"
                     /score="11.9"
                     /tool="antismash"
                     /translation="TRFMVLLTAVQTVLARWS"
     CDS_motif       2386..2475
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0010"
                     /evalue="3.60E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="BM237_RS28540"
                     /protein_end="825"
                     /protein_start="795"
                     /score="51.5"
                     /tool="antismash"
                     /translation="DVPVGTPVAGRGRTELERLVGFFVNTVVLR"
     CDS_motif       2524..2640
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28540_0011"
                     /evalue="1.40E-25"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="BM237_RS28540"
                     /protein_end="880"
                     /protein_start="841"
                     /score="81.8"
                     /tool="antismash"
                     /translation="RATVLGAFDHQDVPFEKLVERLRPERDLSRNPLFQVMVD"
ORIGIN
        1 gtccccctcg atcccgatct gccggtcgag cggctgcggt tcatggtggc cgaggcgggg
       61 gtgggctgtg tcgtggcgga cgcggcgacc gcggggacgg ccgccgggct ggacgcggcc
      121 gtggtacggg ccgacaccgc cggctttgat cccgacggcc cgcgccgcac cccggatccg
      181 cacgacctgg cctacaccat cttcacctcc ggttccaccg gccggcccaa ggcggtcggt
      241 gtggagcacc acgccctggc cgcccatgtc gccgccgccc gcgaccgtta cggcatcacc
      301 gccgaggacc gggtgctggc cttcgcctcc ttctccttcg acgcctccct ggaccaggtc
      361 ctgcccgccc tgacgtgcgg cgcacaggtg atcgtccgcc ccgacgagcc ctggctgccg
      421 acccaggtgc ccgaggtggt cgagcggcac ggcgtcaccg tcgtgaacct cccgcccacc
      481 tactggaacg agctggcgtt ctccctcgac cgccgcgccg ccgcgagact ggccggcctg
      541 cggctgctga tcctgggcgg ggagtccgta ccggccggcg cgctggccgc ctggcgggcc
      601 gaggcccccc atgtgcgggt gtgcaacgcc tacgggccga ccgagacgac cgtcacctcc
      661 accacctacg aggtcaccgg accggtggag ggccgggtgc cgatcggccg cccgctgggc
      721 tcgcgccggg tgtacgtggt ggacgcgcac gaccggcccg tcgacgtcgg cgtccccggc
      781 gaactgctcg tcggcgggcc ggagcttgcg cgcggctatc tgggacggcc ggcgctgacc
      841 gcggagcggt tcgtgcccga tcccttcgcc tccggcggcg gccggctcta ccgcaccggc
      901 gacatcgtcc ggtggctgcc gtccggggac ctggagttcc tcggccggag cgacgaccag
      961 gtcaagatcc gcggcttccg cgtcgaactc ggcgaggtcg aggcggtcct gcgcgagtgc
     1021 ccccgggtgg ccgccgccgc cgtgcggccc gacaccggca ccggtcagac cctgatcggt
     1081 tacgtcgtga ccggacccga cggacccgac ggcacgggcg gcaccgacgg cacgggcgga
     1141 cccgtcgagc cggccgcgct gcgggcctgg tgcgccgagc ggctgccgca ctacgcggtg
     1201 ccctccgacg tcgtgctcct cgacgcgctc cccgtcaacg cctcgggcaa gctcgaccgg
     1261 gccgcgctgc ccgacgcccg ccccgaccgt gccaccggcc gtgtggccca caccgcgccc
     1321 cgcgacgaga gcgagcggat catcgcgtcc gtctgggcgg acgtcctcgg cgtcgaacgc
     1381 gtcggcatcg acgacggctt cttcgacctg ggcggccact cactgctcgc cacgatggcg
     1441 gtctcccgcg tcgccgaacg cctgggccgc gacatcgagc tgcgcaccct gttcgagaac
     1501 ccccgcatcc gggagttcgg cccggcggtg gccgccgcgc ggcgggccgc cgggaccgcc
     1561 gtcacccccg tggaccgctc cggcccgctg ccgatgtcct tcgcccagga gcggctgtgg
     1621 ttcctggacc gcacctccga ggcgggcgac gagtacgtcc tgtggttctc ctggcgcctg
     1681 cgcggcggcc tggaccgcga cgcctggcag gccgcgctgg acgacatgac ggctcggcac
     1741 gaggtgctgc gcactgcgct ggtcgaggtg gacggccgtc ccgcacagca ggtctgcgac
     1801 ccgatgggcg taccgctgga gtggcggcag gcccccgggg gcgaggacga ggcggcccgg
     1861 ctggaagcgg tgcgggagca ggcccacgcg ctcgccaccc gccgcttcga cctggaccgc
     1921 ccgccgatgc tgcgcgccgg ggtgtgggag ctgggaccgc gcgaccacgt cgccgtcctc
     1981 gccttccacc acgtggccac cgacggctgg tccaaggacg tcttcaccgg ggaactggcg
     2041 gcctgctacc gggcgcgtct ggcgggccgg gccccggcac tacccgaacc gcccgtgcag
     2101 tacggcgact tcgcggtgtg gcagcgcgac cgggccgggg acgggacgct ggaggaccgg
     2161 ctggactact gggaggagga actgcgggac ctcccggtgc tgcggctgcc caccgaccgt
     2221 ccccgtcccg cggcctggtc gggccggggc ggctcggtcg aggtgggact gcccgccgaa
     2281 ctggcctccc ggctggacgc cttcgcccgc gagcaggggg cgacgcgctt catggtgctc
     2341 ctgaccgcgg tgcagacggt gctggcccgc tggtccggac aggacgacgt gccggtgggc
     2401 acccccgtcg ccggccgggg ccggacggag ctggaacggc tggtcggctt cttcgtcaac
     2461 accgtcgtcc tgcgcggcga cctgtccggc tctcccacct tcaccgaact gctcgcaagg
     2521 gtccgcgcga ccgtgctggg cgccttcgac caccaggacg tgcccttcga gaagctggtg
     2581 gaacggctgc ggcccgagcg cgacctgtcc cgcaaccccc tgttccaggt gatggtcgac
     2641 gtccaggaga gccccacgtc ggggccggac atcgagggcc tggaggcgac cggcttcgac
     2701 ctgccgtggg cgtcggccaa gttcgacctg accaccacct tcctcctcta tcccgaccgg
     2761 ttcgcactca acgtcgagta cgcggccgac ctcttcgacg ccgccaccgc cacccggctg
     2821 gccgaacacg tgggacgggt gctggccgcc gtcacggccg acccggacat ccccgtccac
     2881 cgggtggacc tgctgtctcc cgccgaacac gcacatctgg tcgccaccgc gggaacggac
     2941 cgtctcgcgg ccggttccgt gcccgttgtg gccgtggggg gtgagccggg tgcgtccgcg
     3001 ctggtgtgcg gggagcggtc ggtgacctac ggcgagctgg ag
//
