LOCUS       NZ_FOSG01000038        15238 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces pini strain PL19, whole genome shotgun sequence.
ACCESSION   NZ_FOSG01000038
VERSION     NZ_FOSG01000038.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces pini
  ORGANISM  Streptomyces pini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900114215.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:14:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,762
            CDSs (total)                      :: 5,677
            Genes (coding)                    :: 5,560
            CDSs (with protein)               :: 5,560
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 20 of 117
            Pseudo Genes (incomplete)         :: 111 of 117
            Pseudo Genes (internal stop)      :: 1 of 117
            Pseudo Genes (multiple problems)  :: 15 of 117
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 15238
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOSG01000038.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..569
                     /locus_tag="BM237_RS28190"
                     /old_locus_tag="SAMN05192584_1381"
     source          1..15238
                     /db_xref="taxon:1520580"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0075229_138"
                     /organism="Streptomyces pini"
                     /strain="PL19"
                     /type_material="type strain of Streptomyces pini"
     region          1..15238
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T2PKS"
                     /region_number="1"
                     /rules="(t2ks and t2clf)"
                     /tool="antismash"
     cand_cluster    1..15238
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2ks and t2clf)"
                     /kind="single"
                     /product="T2PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..15238
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4786:7269](+)"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="8|9 (Score: 711.4; E-value:
                     6.2e-217)"
                     /t2pks_molecular_weights="acetyl-CoA_8 (Da): 351.372"
                     /t2pks_molecular_weights="acetyl-CoA_9 (Da): 393.408"
                     /t2pks_product_classes="angucycline"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
                     /tool="antismash"
     proto_core      4787..7269
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="8|9 (Score: 711.4; E-value:
                     6.2e-217)"
                     /t2pks_molecular_weights="acetyl-CoA_8 (Da): 351.372"
                     /t2pks_molecular_weights="acetyl-CoA_9 (Da): 393.408"
                     /t2pks_product_classes="angucycline"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
     CDS             <5..569
                     /codon_start=3
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948354.1"
                     /locus_tag="BM237_RS28190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_1381"
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="WP_139238201.1"
                     /transl_table=11
                     /translation="LAEFTVEGMATALPFHRTVVADPAFAPELHGRPAGPFDVHTRWIE
                     TEFVNEISPFAAPGADTEDDEPGRETVVVEVGGKRLEVSLPAALGMSLARTAAAGGARP
                     KRRAARKSGPAASGDALASPMQGTIVKIAVEEGQTVAEGELIVVLEAMKMEQPINAHRA
                     GTVKGLTAAVGTGVSAGAVICEIKD"
     gene            complement(726..1628)
                     /locus_tag="BM237_RS28195"
                     /old_locus_tag="SAMN05192584_1382"
     CDS             complement(726..1628)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM237_RS28195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05192584_1382"
                     /product="hypothetical protein"
                     /protein_id="WP_139238202.1"
                     /transl_table=11
                     /translation="MGTGCVRREGAGLARHLPDPGSLLCSPCRGGLLRDLRQLPGLYEE
                     CARQLGGRPVRTGERTSGGPLPGMPFDTRAAEVRSSVLGVLSSWATLVVEERGVTAPRR
                     TVAALAEFLARHADWLSGHRAAAEACEEVARLVRRARRVIDPEPDHRVGIGGCVEPGCS
                     GGLTALLRPERPRLPAEIRCDADPAHRWFGHEWLQLSRRLAPLRASSAAAAAPDSGPDS
                     GPHGRTAPRGSEPAGHEDVRWVTAADVSRLWAIPLGSVYRHASVRKWRRRSRAGRTYYH
                     GADVDRTLSGRGAAAGNGL"
     misc_feature    complement(1775..1789)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.27"
                     /tool="antismash"
     misc_feature    complement(2018..2031)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.41"
                     /tool="antismash"
     gene            2242..3705
                     /locus_tag="BM237_RS28200"
                     /old_locus_tag="SAMN05192584_1383"
     CDS             2242..3705
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_functions="biosynthetic-additional (t2pks) OXY
                     (Score: 617.7; E-value: 3.4e-188)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511390.1"
                     /locus_tag="BM237_RS28200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_1383"
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_093852578.1"
                     /transl_table=11
                     /translation="MDAAVIVVGAGPAGLMLAAELRVAGVDVIVVERLARRTGESRGLG
                     FTARTMEVFDQRGILPRFGGIETSNVGHFGGLPVDFGVLEGAWQAAKTVPQSETEKHLE
                     EWAVSLGADIRRGHEMLSLTDDGEGVDVEVRGPEGVHTLRAAYLVGCDGGRSAVRKAAG
                     FDFPGTPSTMEMFLADLKGIELAPRMIGETLPGGMVMVGPLPGGITRIIVCERGTPPQR
                     RETPPSFEEVADAWKRLTGEDISHAEPVWVSSFGDATRQVTEYRRGRVLLAGDSAHIHL
                     PAGGQGMNTSIQDSVNLGWKLAAEIKGWAPRELLDTYHTERHAVGKRLLMNTRAQGLLF
                     LSGPEVQPLRDVLTELIGYRDVSRHLAAMVSGLEITYDVGGGTHPLLGRRMPHLRLDAD
                     RPTSSTELLHTGRGVLLDLEDNRRLRSRAAGWADRVDTVTARPQDAPEELRNTAAVLVR
                     PDGYIAWAAPGSHHDLPMALERWFGRPRG"
     misc_feature    3732..3747
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.59"
                     /tool="antismash"
     gene            3733..4398
                     /locus_tag="BM237_RS28205"
                     /old_locus_tag="SAMN05192584_1384"
     CDS             3733..4398
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268983.1"
                     /locus_tag="BM237_RS28205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_1384"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_093852579.1"
                     /transl_table=11
                     /translation="MTTILDAALTTVRAPGPAEERRHVVRAARGVFAREGWTHTTVEAV
                     AAEAGTGPETVEHYFKDKGRLLLAVLLEGAAAVAAALTAVAEEHLAEVTDLERDLAALG
                     RAWLTPLIAFPEHFAIVRHIGAETARLPAGVLEMWQSAGPRQTQRELARRLRGLADAGL
                     LDVADADHAADCFVRLVVGGVVQRSFHGAVPLSDFETDALIESGVADFVRLFGPATAR"
     gene            4420..4749
                     /locus_tag="BM237_RS28210"
                     /old_locus_tag="SAMN05192584_1385"
     CDS             4420..4749
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1225: Polyketide synthesis cyclase"
                     /gene_functions="biosynthetic-additional (t2pks) CYC
                     C4-C17/C2-C19 (Score: 201.5; E-value: 2.8e-62)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019767153.1"
                     /locus_tag="BM237_RS28210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_1385"
                     /product="TcmI family type II polyketide cyclase"
                     /protein_id="WP_093852580.1"
                     /transl_table=11
                     /translation="MHSTLIVARMDIDSSAEVARIFAEFDKTDMPHRMGTRRRQLFAYQ
                     GLYFHLQDFDGDNGGERIEEARTDPRFVRISEDLKPFIEAYDPKTWRSPADAMATRFYD
                     WTASR"
     gene            4787..6055
                     /locus_tag="BM237_RS28215"
                     /old_locus_tag="SAMN05192584_1386"
     CDS             4787..6055
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (4-396). E-value:
                     6e-57. Score: 185.3. Matches aSDomain:
                     nrpspksdomains_BM237_RS28215_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_functions="biosynthetic-additional (t2pks) KS (Score:
                     804.1; E-value: 5.7e-245)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385254.1"
                     /locus_tag="BM237_RS28215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_1386"
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="WP_093852581.1"
                     /sec_met_domain="t2ks (E-value: 1e-255, bitscore: 838.2,
                     seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 4.2e-60, bitscore:
                     193.3, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGRRVVITGIGVLAPGGIGAKNFWSLLSEGRTATRRISFFDPSPF
                     RSRVAAEADFDPELHGLSPQEIRRMDRAAQFAVVTAREALADSGLDLAEYDPHRTGVTI
                     GSAVGATTGLDDEYRVVSDGGRLDLVDHTYAPQHLYNYFVPSSFSAEVAWAVGAEGPVT
                     VVSTGCTSGIDSVGHAVELIREGTADVMVSGATDAPISPITMACFDAIKATTPRNDDPE
                     HASRPFDGTRNGFVLGEGAAVFVLEELESARRRGAHIYAEIAGYASRCNAFHMTGLRPD
                     GREMAEAIRVAMAEARMAPESIDYINAHGSGTKQNDRHETAAFKLSLGEHAYRTPVSSI
                     KSMVGHSLGAIGSIEIAASALAMENAMVPPTANLHTPDPECDLDYVPLTAREQRTDAVL
                     TVGSGFGGFQSAMVLARPERSAA"
     aSDomain        4799..5974
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: False"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28215_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="6.00E-57"
                     /label="BM237_RS28215_PKS_KS.1"
                     /locus_tag="BM237_RS28215"
                     /protein_end="396"
                     /protein_start="4"
                     /score="185.3"
                     /tool="antismash"
                     /translation="VVITGIGVLAPGGIGAKNFWSLLSEGRTATRRISFFDPSPFRSRV
                     AAEADFDPELHGLSPQEIRRMDRAAQFAVVTAREALADSGLDLAEYDPHRTGVTIGSAV
                     GATTGLDDEYRVVSDGGRLDLVDHTYAPQHLYNYFVPSSFSAEVAWAVGAEGPVTVVST
                     GCTSGIDSVGHAVELIREGTADVMVSGATDAPISPITMACFDAIKATTPRNDDPEHASR
                     PFDGTRNGFVLGEGAAVFVLEELESARRRGAHIYAEIAGYASRCNAFHMTGLRPDGREM
                     AEAIRVAMAEARMAPESIDYINAHGSGTKQNDRHETAAFKLSLGEHAYRTPVSSIKSMV
                     GHSLGAIGSIEIAASALAMENAMVPPTANLHTPDPECDLDYVPLTAREQRTD"
     gene            6052..7269
                     /locus_tag="BM237_RS28220"
                     /old_locus_tag="SAMN05192584_1387"
     CDS             6052..7269
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (6-404). E-value:
                     2.8e-30. Score: 97.5. Matches aSDomain:
                     nrpspksdomains_BM237_RS28220_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093: Beta-ketoacyl synthase"
                     /gene_functions="biosynthetic-additional (t2pks) CLF 8|9
                     (Score: 711.4; E-value: 6.2e-217)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037162.1"
                     /locus_tag="BM237_RS28220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_1387"
                     /product="ketosynthase chain-length factor"
                     /protein_id="WP_093852582.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 3.4e-45, bitscore:
                     144.5, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="t2clf (E-value: 6.9e-216, bitscore: 706.4,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNARAVVTGIGVATPNGMGVEDFWAATRVGKNAIGPVTRFDASRY
                     PSRLAGEIRGFEAADHLPNRLIPQTDRMTQLALVAADCAFADAGVEPGDIPEFDMGVVT
                     ASTCGGFEFGQNELKKLWSQGSQYVSAYQSFAWFYAVNSGQISIRNGMRGPSGVVVSDH
                     AGGLDAIAQARRQIRKGSRLIFSGGFDAPVCPWGWVAQIAGGRLSTGDDPETAYLPFDS
                     RARGYVPGEGGALLILEEERTARERGAENVYGVIAGYGATFDPRPGSGREPGLRRAAEL
                     ALADAGVRPRDVDVVFADAAGTPSLDREEAEAITAVFGPFGVPVTAPKTMTGRLYSGAA
                     PVDVVSALLAVREGLIPPTTNVERSAEYDIDLVTGQPRTAPVRTALVLARGHGGHNSAV
                     VVRAAD"
     aSDomain        6070..7263
                     /ASF="found active site histidines: False"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28220_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="2.80E-30"
                     /label="BM237_RS28220_PKS_KS.1"
                     /locus_tag="BM237_RS28220"
                     /protein_end="404"
                     /protein_start="6"
                     /score="97.5"
                     /tool="antismash"
                     /translation="VTGIGVATPNGMGVEDFWAATRVGKNAIGPVTRFDASRYPSRLAG
                     EIRGFEAADHLPNRLIPQTDRMTQLALVAADCAFADAGVEPGDIPEFDMGVVTASTCGG
                     FEFGQNELKKLWSQGSQYVSAYQSFAWFYAVNSGQISIRNGMRGPSGVVVSDHAGGLDA
                     IAQARRQIRKGSRLIFSGGFDAPVCPWGWVAQIAGGRLSTGDDPETAYLPFDSRARGYV
                     PGEGGALLILEEERTARERGAENVYGVIAGYGATFDPRPGSGREPGLRRAAELALADAG
                     VRPRDVDVVFADAAGTPSLDREEAEAITAVFGPFGVPVTAPKTMTGRLYSGAAPVDVVS
                     ALLAVREGLIPPTTNVERSAEYDIDLVTGQPRTAPVRTALVLARGHGGHNSAVVVRAA"
     CDS_motif       6709..6747
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28220_0001"
                     /evalue="7.10E-04"
                     /label="PKSI-KS_m4"
                     /locus_tag="BM237_RS28220"
                     /protein_end="232"
                     /protein_start="219"
                     /score="12.7"
                     /tool="antismash"
                     /translation="FDSRARGYVPGEG"
     misc_feature    7329..7345
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.55"
                     /tool="antismash"
     gene            7389..7661
                     /locus_tag="BM237_RS28225"
                     /old_locus_tag="SAMN05192584_1388"
     CDS             7389..7661
                     /NRPS_PKS="Domain: ACP (12-83). E-value: 1.5e-17. Score:
                     55.7. Matches aSDomain: nrpspksdomains_BM237_RS28225_ACP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1129: acyl carrier protein"
                     /gene_functions="biosynthetic-additional (t2pks) ACP
                     (Score: 139.6; E-value: 5.6e-44)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141377.1"
                     /locus_tag="BM237_RS28225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_1388"
                     /product="acyl carrier protein"
                     /protein_id="WP_093852583.1"
                     /sec_met_domain="PP-binding (E-value: 1.4e-13, bitscore:
                     41.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPEQKFTLEDLKRILREGAGTDEGVDLDGDILDTDFEELGYESLA
                     LLETGGRIEREYGISLDDDVFTENRTPRSLIDAVNEHLAAILTHS"
     aSDomain        7425..7637
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28225_ACP.1"
                     /evalue="1.50E-17"
                     /label="BM237_RS28225_ACP.1"
                     /locus_tag="BM237_RS28225"
                     /protein_end="83"
                     /protein_start="12"
                     /score="55.7"
                     /tool="antismash"
                     /translation="RILREGAGTDEGVDLDGDILDTDFEELGYESLALLETGGRIEREY
                     GISLDDDVFTENRTPRSLIDAVNEHL"
     gene            7790..8572
                     /gene="fabG"
                     /locus_tag="BM237_RS28230"
                     /old_locus_tag="SAMN05192584_1389"
     CDS             7790..8572
                     /EC_number="1.1.1.100"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (7-161). E-value: 4.8e-11. Score:
                     34.8. Matches aSDomain:
                     nrpspksdomains_BM237_RS28230_PKS_KR.1"
                     /codon_start=1
                     /gene="fabG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_functions="biosynthetic-additional (t2pks) KR C9
                     (Score: 488.0; E-value: 1.4e-149)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103042.1"
                     /locus_tag="BM237_RS28230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_1389"
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="WP_093852584.1"
                     /sec_met_domain="adh_short (E-value: 4.3e-39, bitscore:
                     124.3, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQQNKRVAVVTGATSGIGLAVARLLARDHKVFIGARNAENVART
                     VKELQGEGLEVDGTTVDVRSADEVRAFVQAAVDRFGTVDVLVNNAGRSGGGVTADIDDE
                     LWEDVVDTNLNSVFRMTREVLNTGGMRGKDRGRIINIASTAGKQGVVLGAPYSASKHGV
                     VGFTKALGNELAPTGITVNAVCPGYVETPMAQRVRQGYAAAYDTTEEAILEKFQAKIPL
                     GRYSTPEEVAGLVGYLASDTAASITSQALNVCGGLGNF"
     aSDomain        7811..8272
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28230_PKS_KR.1"
                     /evalue="4.80E-11"
                     /label="BM237_RS28230_PKS_KR.1"
                     /locus_tag="BM237_RS28230"
                     /protein_end="161"
                     /protein_start="7"
                     /score="34.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VAVVTGATSGIGLAVARLLARDHKVFIGARNAENVARTVKELQGE
                     GLEVDGTTVDVRSADEVRAFVQAAVDRFGTVDVLVNNAGRSGGGVTADIDDELWEDVVD
                     TNLNSVFRMTREVLNTGGMRGKDRGRIINIASTAGKQGVVLGAPYSASKH"
     CDS_motif       7817..7873
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BM237_RS28230_0001"
                     /evalue="8.20E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="BM237_RS28230"
                     /protein_end="28"
                     /protein_start="9"
                     /score="18.5"
                     /tool="antismash"
                     /translation="VVTGATSGIGLAVARLLAR"
     gene            8744..9691
                     /locus_tag="BM237_RS28235"
                     /old_locus_tag="SAMN05192584_13810"
     CDS             8744..9691
                     /NRPS_PKS="Domain: Polyketide_cyc2 (7-149). E-value:
                     1.4e-14. Score: 46.7. Matches aSDomain:
                     nrpspksdomains_BM237_RS28235_Polyketide_cyc2.1"
                     /NRPS_PKS="Domain: Polyketide_cyc2 (158-311). E-value:
                     2.4e-10. Score: 32.9. Matches aSDomain:
                     nrpspksdomains_BM237_RS28235_Polyketide_cyc2.2"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128: cyclase/dehydrase"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C7-C12
                     (Score: 503.0; E-value: 5.7e-154)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269009.1"
                     /locus_tag="BM237_RS28235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_13810"
                     /product="aromatase/cyclase"
                     /protein_id="WP_093852585.1"
                     /transl_table=11
                     /translation="MSRSALREVEHEITVSAPAATVYRLIAEVENWPRIFPPTIYVDHV
                     ERNEDSERIRIWATANGEAKNWTSRRVLDPENLRIGFRQEVSAPPVASMGGTWVVEALS
                     ETESRVRLLHDYRAVDDDPESLRWIDEAVDRNSRSELAALKANIEQAHAAEDLTFSFED
                     SVRIAGSAKDAFDFVNEAGLWPERLPHVATVRFEEETPGLQTLEMDTRAKDGSVHTTKS
                     YRVAFPHHRIAYKQVTLPALMTLHTGYWTFEQDDQGVTTATSQHTVTLNTANIERILGP
                     EATVEDAREYVRSALSTNSRATLGHAKDYAEARA"
     aSDomain        8765..9190
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28235_Polyketide_cyc2.1"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="1.40E-14"
                     /label="BM237_RS28235_Polyketide_cyc2.1"
                     /locus_tag="BM237_RS28235"
                     /protein_end="149"
                     /protein_start="7"
                     /score="46.7"
                     /tool="antismash"
                     /translation="EVEHEITVSAPAATVYRLIAEVENWPRIFPPTIYVDHVERNEDSE
                     RIRIWATANGEAKNWTSRRVLDPENLRIGFRQEVSAPPVASMGGTWVVEALSETESRVR
                     LLHDYRAVDDDPESLRWIDEAVDRNSRSELAALKANIE"
     aSModule        8765..9676
                     /domains="nrpspksdomains_BM237_RS28235_Polyketide_cyc2.1"
                     /domains="nrpspksdomains_BM237_RS28235_Polyketide_cyc2.2"
                     /incomplete
                     /locus_tags="BM237_RS28235"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        9218..9676
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28235_Polyketide_cyc2.2"
                     /domain_subtypes="Polyketide_cyc2"
                     /evalue="2.40E-10"
                     /label="BM237_RS28235_Polyketide_cyc2.2"
                     /locus_tag="BM237_RS28235"
                     /protein_end="311"
                     /protein_start="158"
                     /score="32.9"
                     /tool="antismash"
                     /translation="FSFEDSVRIAGSAKDAFDFVNEAGLWPERLPHVATVRFEEETPGL
                     QTLEMDTRAKDGSVHTTKSYRVAFPHHRIAYKQVTLPALMTLHTGYWTFEQDDQGVTTA
                     TSQHTVTLNTANIERILGPEATVEDAREYVRSALSTNSRATLGHAKDYA"
     gene            9694..12021
                     /locus_tag="BM237_RS28240"
                     /old_locus_tag="SAMN05192584_13811"
     CDS             9694..12021
                     /NRPS_PKS="Domain: PKS_KR (529-707). E-value: 2.2e-09.
                     Score: 29.4. Matches aSDomain:
                     nrpspksdomains_BM237_RS28240_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_functions="biosynthetic-additional (t2pks) KR C15
                     (Score: 513.5; E-value: 2.2e-157)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511397.1"
                     /locus_tag="BM237_RS28240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_13811"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_281253986.1"
                     /transl_table=11
                     /translation="MPGPFTETQVIVVGAGPVGLMLAGELRLAGADVVVLERLTAPTTE
                     SRASTLHARTVEILDGRGLLEPLGDVPNDPRGHFGGIPLDLTLPSSHPGQWKVPQTRIE
                     ELLGRWAADLGADVRRGHELTGLTASRDHVEVEAVTAGGATVRLRAGYVVGCDGEDSTV
                     RRLGGFDFPGTDAVRELLRADVSGIEIPDRRFQRLEGGLAIAARRPDGVTRVMVHEFGA
                     VPRRGQPRFADVADAWKRVTGEDIGGGTPLWVNSFGDASRQVTAYRRDRVLLAGDAAHR
                     QMPIGGQALNLGLQDAVNLGWKLAAQVRGHAPEGLLDSYHAERHPVGRRVLDNIRSQAL
                     LLLGGPEVEAVREVLAELTGGEDARRHLAAMVSGLDVRHDVGPGDHPLLGRRLPHWELA
                     TGEGRVTTAELLRTGHGLLLLLPGARERLEELRTAAAPWAERVRTVTARPVPGGQEAPD
                     TAALLVRPDGHVVWADGDPAGTGVTAALNRWFGAGRRADGPAARKDRAPAWTATRAPAT
                     KWRTDMGRLTGKTALVTGSSRGMGRAAALRLAADGALVAVHYTSNEEAAAEVVELIEKD
                     GGRAFTVRAELGVPGDVHELFLGLEEGLRERTGGTDLNILVNNAGVMGGVKPEETTPEK
                     FDRVFAVNARAPFFLIQRALKNMPDGGRIINISSGLTRFANPDEIAYAMSKGAVEQLAL
                     HFAKYLGPRGITVNSVAPGITRNDNPVFQIPEAVEQMSGLSAFGRVGEPEDVADVVAFL
                     ASDDARWITGSFVDATGGTLLG"
     aSDomain        11281..11814
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28240_PKS_KR.1"
                     /evalue="2.20E-09"
                     /label="BM237_RS28240_PKS_KR.1"
                     /locus_tag="BM237_RS28240"
                     /protein_end="707"
                     /protein_start="529"
                     /score="29.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGMGRAAALRLAADGALVAVHYTSNEEAAAEVVELIE
                     KDGGRAFTVRAELGVPGDVHELFLGLEEGLRERTGGTDLNILVNNAGVMGGVKPEETTP
                     EKFDRVFAVNARAPFFLIQRALKNMPDGGRIINISSGLTRFANPDEIAYAMSKGAVEQL
                     ALHFAKYLGPRGITV"
     gene            12136..13566
                     /locus_tag="BM237_RS28245"
                     /old_locus_tag="SAMN05192584_13812"
     CDS             12136..13566
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610259.1"
                     /locus_tag="BM237_RS28245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_13812"
                     /product="MFS transporter"
                     /protein_id="WP_093852586.1"
                     /transl_table=11
                     /translation="MPENNPSVPAAATAGGGWTPALWGLLLVLAGNMLIDALEVSVALV
                     ALPAIGDDLGLAPDRLHWVVSGFAAGFGGVLLLGGRVVALLGRRPVYLTALAVFAAASL
                     AGALAEDPALLIATRFVKGVCAALTAPTGLAIILSAFPQGPARNRAVSVYSLFGAAGFA
                     TGLLLSGALTEIGWRWTFAFPAPVALLLFAAAVRLVPGDPPDRSGGGSGLAGAAGLTVV
                     TLLLVHAIGSVPRTGWGAPSTAGAFALAGVLAVLLARRELTGPRPLADARLLTHAPLAR
                     SALGAAALNGSHIGILMLSALHLQRHAGLGPWQTGLAFLPASAPLVLSALYSGRMVARL
                     GPARPIAAGAAAAALGYALYPRDPSAPLEYATDVLPTMLLIGAAFVLAFTALHVQATST
                     APAGRQAEAGGLYQTAVQLGAALTTALTAAVYTAAGHGAAVLLVTAVGAAGLVVALPGL
                     RRETRTDPLPADLTRRRT"
     gene            13563..13958
                     /locus_tag="BM237_RS28250"
                     /old_locus_tag="SAMN05192584_13813"
     CDS             13563..13958
                     /NRPS_PKS="Domain: PKS_PP (35-117). E-value: 1e-08. Score:
                     27.4. Matches aSDomain:
                     nrpspksdomains_BM237_RS28250_PKS_PP.1"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /locus_tag="BM237_RS28250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_13813"
                     /product="acyl carrier protein"
                     /protein_id="WP_093852587.1"
                     /transl_table=11
                     /translation="MTANPTAERAAHTRAAPKAPAGAAAPDAAPDRGVRAAADHQDRIR
                     ELDAYVRRLIGRVLGVAPHLVDTAGRPMNSLGIGSIAGLELQRRMERDPHVRVDLRRLL
                     LAGSAAELVDCPAGQLRGRRHTGVDPA"
     aSDomain        13668..13913
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28250_PKS_PP.1"
                     /evalue="1.00E-08"
                     /label="BM237_RS28250_PKS_PP.1"
                     /locus_tag="BM237_RS28250"
                     /protein_end="117"
                     /protein_start="35"
                     /score="27.4"
                     /tool="antismash"
                     /translation="AAADHQDRIRELDAYVRRLIGRVLGVAPHLVDTAGRPMNSLGIGS
                     IAGLELQRRMERDPHVRVDLRRLLLAGSAAELVDCPA"
     gene            13955..14779
                     /locus_tag="BM237_RS28255"
                     /old_locus_tag="SAMN05192584_13814"
     CDS             13955..14779
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: ACPS (142-206). E-value: 6.1e-08. Score:
                     24.5. Matches aSDomain:
                     nrpspksdomains_BM237_RS28255_ACPS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012: 4'-phosphopantetheinyl transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511399.1"
                     /locus_tag="BM237_RS28255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_13814"
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="WP_245794101.1"
                     /transl_table=11
                     /translation="MTPPATGPAAGGARPHPAAGEAEFTVARGRLDLWVVRSPEGRTAR
                     ELDRSVLDDAERRRADMFLRPADGLLYTAAHIALRRLLGRYLGVAAEEVRFFREPCPGC
                     GGPHGRPAAAPPDPTDPAPHFSLSHSGGLAVVGVAAVPVGVDVERLPGEETVEVCTPAL
                     HPGERAELAEVGGEARRELFGRLWTRKEAYLKGLGTGLGRAPDRDYLGADVRRHPRGWT
                     VLDVLRTPTHTAAAAVRGREPAVVAVRPLAETCLLAGGEARSARPEPAPAAR"
     aSDomain        14381..14572
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS28255_ACPS.1"
                     /evalue="6.10E-08"
                     /label="BM237_RS28255_ACPS.1"
                     /locus_tag="BM237_RS28255"
                     /protein_end="206"
                     /protein_start="142"
                     /score="24.5"
                     /tool="antismash"
                     /translation="VGVDVERLPGEETVEVCTPALHPGERAELAEVGGEARRELFGRLW
                     TRKEAYLKGLGTGLGRAPD"
     gene            14925..>15238
                     /locus_tag="BM237_RS28260"
                     /old_locus_tag="SAMN05192584_13815"
     CDS             14925..>15238
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141373.1"
                     /locus_tag="BM237_RS28260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_13815"
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="WP_281253987.1"
                     /transl_table=11
                     /translation="MSEENETIHHRIEELAEIKETARRGPDPAATERQHARGKLTAHER
                     IGLLLDKGTFTEVEPLRRHRATGFGLEDRKPYTDGVVTGWGTVHGRTVFVYAHDFRIFG
                     G"
     misc_feature    14967..14980
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
ORIGIN
        1 ccctggcgga gttcaccgtg gagggcatgg ccaccgccct gcccttccac cgcacggtcg
       61 tcgccgaccc cgccttcgcc cccgaactgc acggccgccc ggccggcccc ttcgatgtgc
      121 acacccggtg gatcgagacc gagttcgtca atgagatcag cccgttcgcg gcgcccggcg
      181 cggacaccga ggacgacgag cccggccgcg agacggtggt cgtcgaggtc ggcggcaagc
      241 gcctggaggt ctctctgccc gccgcgctgg gcatgtccct ggcccgcacc gccgcggccg
      301 gcggcgcccg ccccaagcgc cgcgcggcca ggaagtccgg cccggccgcc tccggagacg
      361 cgctggcctc cccgatgcag ggcaccatcg tcaagatcgc cgtcgaggag ggccagaccg
      421 tcgccgaggg cgagctgatc gtggtgctgg aggcgatgaa gatggaacag cccatcaacg
      481 cccaccgcgc cggcaccgtc aagggcctga ccgccgccgt cggcaccggc gtcagcgccg
      541 gcgccgtcat ctgcgagatc aaggactgaa cccggaccgg gcccgccccc tcacgtcgcg
      601 gaagcggcgc gagggggcgg gaaagggccg tgctcgccgg atgtctgtcc ggcgggcacg
      661 gccctctgga gccgcggggc ccccgggccc cgcggctcca cggctgttca gggcgttcag
      721 ggcggtcaga gcccgttccc cgcggcggcg ccgcgcccgc tgagcgtccg gtcgacgtcc
      781 gccccgtggt agtaggtccg gcccgcgcgg gaccggcggc gccacttgcg cacgctggcg
      841 tggcggtaga cgctgccgag cgggatcgcc cacagccggg agacgtccgc cgcggtcacc
      901 cagcggacgt cctcgtggcc ggccggttcg gaaccccgcg gcgccgtgcg gccgtgcggc
      961 ccggagtccg gccccgagtc cggggcggcg gccgcggcgg aggacgcccg cagaggcgcc
     1021 agacgccggc tcagctggag ccactcgtgg ccgaaccagc ggtgcgcggg gtcggcgtcg
     1081 cacctgatct cggccggcag ccgcggccgc tccggacgca gcagcgccgt cagcccgccc
     1141 gagcagcccg gctccacaca gccgccgatc cccacccggt ggtccggctc ggggtcgatc
     1201 acccggcgcg cccggcgcac cagccgggcc acctcctcgc aggcctccgc ggcggcccgg
     1261 tggccggaga gccagtcggc gtggcgggcc aggaactccg cgagcgcggc gaccgtgcgg
     1321 cggggggccg tcacaccgcg ctcctcgacc acgagtgtgg cccacgagct cagcacgccg
     1381 aggaccgagg aacgcacctc ggccgcgcgg gtgtcgaagg gcatgccggg cagcggaccg
     1441 ccggacgtcc tctcccccgt ccggaccggc cggccgccga gctgccgggc gcactcctcg
     1501 tacagaccgg gcagctgccg cagatcccgc aggaggcctc cccggcaggg agaacagagc
     1561 aggctgcccg ggtccgggag gtgccgggcc agaccggccc cctcccggcg cacgcagccg
     1621 gttcccacac acgccgtcgt gccgacggcc ccatggatcg tccctcggac cacagtgtcc
     1681 cccagatgtc gttgtcgtca ggagcggcaa ctctaattac ggacgctgtc accggacaac
     1741 aggaattccg tttcctgtgc tcttcctgac gtggagacaa ggaatttcac cgatttcttc
     1801 agttggtccg cgcattccgt gtccgccgtg cgccggggtc gccgcgtgag gtgccggggc
     1861 atgcgcgcgg cagccgggcg gacggccccg gcggttttcc ggaatgccgg gcgccggatg
     1921 tccgggcggc ctctccccgg tgcctcggac ccggcctcgc cccgccctgc gggccaccgc
     1981 ggttccatga gaccccccgc ggaaccgcac tttgcgccga tctgatgttc gtcttgacta
     2041 cgtctgtccg acgtgcgaag gcataacgga atcttgcgta gacctgaatt gtcgagcatt
     2101 gcggtcggcc gtccgggcga agaagcttgt cgctagtttc cgatgctctg tcttgagcgc
     2161 ttgccgaccg gcgaagacgc cccgtgggcg cgccgatttc cccactcttg caggaattcc
     2221 gagaaggccg aggagaggta catggatgct gcggtaatcg tcgtgggagc gggcccggca
     2281 gggctgatgc tcgccgcgga actgcgggtc gcgggagtcg acgtcatcgt tgtggagcgc
     2341 ctggcgcggc gcacggggga gtcccggggc ctgggcttca ccgcccggac catggaggtc
     2401 ttcgaccagc ggggcatcct gccccggttc ggcgggatcg agaccagcaa cgtgggccac
     2461 ttcggcggcc tgcccgtcga cttcggcgtg ctggaggggg cgtggcaggc ggccaagacc
     2521 gtgccccagt ccgagaccga gaagcacctg gaggaatggg ccgtcagcct cggcgcggac
     2581 atccgccgcg gccacgagat gctctccctc acggacgacg gcgagggcgt cgacgtggag
     2641 gtccgcggcc ccgagggcgt ccacaccctg cgcgccgcct acctggtggg ctgcgacgga
     2701 ggacggagcg cggtgcgcaa ggccgccggc ttcgacttcc ccggcacgcc ctccacgatg
     2761 gagatgttcc tcgccgacct caagggcatc gagctcgcgc cgcgcatgat cggcgagacc
     2821 ctgcccggcg gcatggtcat ggtcggcccg ctgccgggcg gcatcacgcg catcatcgtc
     2881 tgcgagcggg gcaccccgcc ccagcggcgc gagacgcccc cctccttcga ggaggtcgcc
     2941 gacgcctgga agcggctgac cggcgaggac atctcgcacg ccgaaccggt gtgggtcagc
     3001 tccttcggcg acgccacgcg ccaggtcacc gagtaccgcc gcggccgcgt cctcctggcc
     3061 ggcgactcgg cgcacatcca cctgcccgcc ggcgggcagg gcatgaacac cagcatccag
     3121 gactccgtga acctcggctg gaagcttgcc gccgagatca agggctgggc cccgcgggaa
     3181 ctgctggaca cgtaccacac cgagcgccac gcggtcggca agcgactgct gatgaacacc
     3241 cgggcccagg ggctgctctt cctcagcgga cccgaggtgc agccgctgcg cgacgtgctc
     3301 accgagctca tcgggtaccg ggacgtcagc aggcacctgg ccgccatggt gagcggcctg
     3361 gagatcacct acgacgtcgg gggcggcacc cacccgctgc tcggccgccg catgccgcac
     3421 ctccggctcg acgccgaccg cccgaccagc agcaccgagc tgctgcacac cgggcgcggc
     3481 gtgctgctgg acctggagga caaccggcgc ctgcggagcc gggccgccgg ctgggcggac
     3541 cgcgtcgaca ccgtcaccgc caggccgcag gacgcacccg aggagctccg gaacaccgcc
     3601 gccgtgctgg tccgccccga cggctacatc gcctgggccg cccccggcag ccaccacgac
     3661 cttcccatgg ccctggagcg ctggttcggc cgcccccgcg gctgagagcg gcccgccgaa
     3721 gtggaggaac ccatgacgac cattctcgac gcggcgctca ccaccgtccg ggcgccgggc
     3781 cccgcggagg agcgccggca cgtcgtacgg gccgcccgcg gcgtcttcgc ccgcgagggc
     3841 tggacgcaca ccaccgtcga ggccgtggcc gccgaggcgg gcaccgggcc ggagacggtg
     3901 gagcactact tcaaggacaa ggggcggctc ctgctggccg tcctgctcga aggcgccgcc
     3961 gcggtcgccg ccgccctgac cgccgtggcc gaggagcacc tcgccgaggt caccgacctg
     4021 gagcgggacc tcgccgccct cggccgggcc tggctgacac cgctgatcgc cttccccgag
     4081 cacttcgcca tcgtccggca catcggcgcg gagaccgccc ggctgcccgc cggggtgctg
     4141 gagatgtggc agtcggcggg cccccggcag acccagcggg agctggcgcg ccgcctgcgg
     4201 ggcctggccg acgcgggact gctggacgtg gccgacgccg accacgcggc cgactgcttc
     4261 gtccggctgg tcgtgggagg cgtcgtccag cgctccttcc acggcgccgt gcccctttcg
     4321 gacttcgaga ccgacgcgct catcgagagc ggggtggccg acttcgtccg cctgttcggt
     4381 cccgcgaccg cccgctgatc ccacggaaga gagaagacga tgcacagcac cctgatcgtg
     4441 gcccggatgg acatcgactc cagcgccgag gtcgccagga tcttcgccga gttcgacaag
     4501 accgacatgc cgcaccgcat gggcacccgc cgccgccagc tcttcgccta ccagggcctg
     4561 tacttccacc tgcaggactt cgacggcgac aacggcggcg agcggatcga ggaggccagg
     4621 acggaccccc ggttcgtgcg catcagcgag gacctcaagc cgttcatcga ggcgtacgac
     4681 ccgaagacct ggcggtcccc ggccgacgcg atggccaccc gcttctacga ctggacggcc
     4741 tcgcggtgac gaggcggcac atctacacga ggggaggcca cgaccagtgg gtcgccgggt
     4801 agtgatcacg gggatcggag tcctggcgcc gggcgggatc ggcgccaaga acttctggag
     4861 tctgctgagc gagggccgca cggcgacccg gcggatctcc ttcttcgatc cctcaccgtt
     4921 ccgttccagg gtcgcggcgg aggccgactt cgaccccgag ctgcacgggc tgtctccgca
     4981 ggagatccgc cgcatggacc gcgccgccca gttcgccgtg gtcaccgcgc gcgaggcgct
     5041 ggccgacagc ggcctggatc tggcggagta cgacccgcac cgcaccggtg tcaccatcgg
     5101 cagcgcggtc ggcgcgacca ccgggctcga cgacgagtac cgggtcgtca gcgacggggg
     5161 ccggctcgac ctggtcgacc acacgtacgc cccgcagcac ctgtacaact acttcgtgcc
     5221 cagctccttc tcggcggagg tggcctgggc cgtcggcgcc gagggacccg tgaccgtcgt
     5281 gtccaccggc tgcacctccg gcatcgactc ggtcggccac gccgtcgagc tgatccgcga
     5341 gggcaccgcc gacgtgatgg tctccggcgc cacggacgcc ccgatctcgc cgatcaccat
     5401 ggcgtgcttc gacgcgatca aggcgaccac gccgcgcaac gacgaccccg agcacgcctc
     5461 gcggcccttc gacgggaccc gcaacggctt cgtgctcggc gagggcgccg cggtgttcgt
     5521 cctggaggag ctggagagcg cacgcaggcg cggggcgcac atctacgccg agatcgccgg
     5581 ctacgcctcc cgctgcaacg ccttccacat gacgggcctg cgccccgacg gccgcgagat
     5641 ggccgaggcg atcagggtgg cgatggccga ggcgcggatg gcccccgagt ccatcgacta
     5701 catcaacgcg cacggctccg gaacgaagca gaacgaccgc cacgagaccg ccgcgttcaa
     5761 gctcagcctg ggcgagcacg cctaccgcac gccggtcagc tccatcaagt cgatggtggg
     5821 gcactcgctg ggtgcgatcg gctcgatcga gatcgcggcc tcggcgctgg cgatggagaa
     5881 cgcgatggtg ccgcccaccg ccaacctgca cacccccgac cccgagtgcg acctggacta
     5941 cgtgccgctg accgcgcgcg agcagcgcac ggacgcggtg ctgacggtcg gcagcggatt
     6001 cggcgggttc cagagcgcga tggtgctggc ccgccccgag aggagtgcgg catgaacgcc
     6061 agggcagtgg tcaccgggat cggcgtcgcc acccccaacg gaatgggagt ggaggacttc
     6121 tgggccgcca cccgggtcgg gaagaacgcg atcggtccgg tgacccgctt cgacgcgagc
     6181 cggtacccgt cacggctggc gggcgagatc cgcggcttcg aggccgcgga ccacctgccg
     6241 aaccggctga tcccgcagac ggaccggatg acgcagctgg ccctggtcgc cgccgactgc
     6301 gccttcgccg acgcgggcgt cgagcccggg gacatccccg agttcgacat gggcgtggtc
     6361 accgcctcca cctgcggcgg cttcgagttc ggccagaacg agctcaagaa gctctggagc
     6421 cagggaagtc agtacgtcag cgcctaccag tccttcgcct ggttctacgc cgtcaacagc
     6481 ggccagatct ccatccgcaa cggcatgcgc ggccccagcg gcgtggtcgt cagcgaccac
     6541 gcgggcggtc tggacgccat cgcgcaggcc cgccggcaga tccgcaaggg cagcaggctg
     6601 atcttctccg gcggcttcga cgcgccggtc tgcccctggg ggtgggtcgc gcagatcgcc
     6661 ggaggcaggc tgtccaccgg cgacgacccc gagaccgcct acctgccctt cgactcccgt
     6721 gcccggggat acgtgcccgg ggagggcggc gcgctcctga tcctggagga ggagcggacc
     6781 gcccgggagc gcggcgcgga gaacgtctac ggcgtgatcg ccggctacgg ggcgaccttc
     6841 gaccccaggc cgggcagcgg ccgcgagccg ggcctgcgcc gggccgccga actcgccctc
     6901 gccgacgccg gggtgcggcc gcgggacgtc gacgtcgtct tcgccgacgc ggccggcacc
     6961 ccctcgctgg accgcgagga ggccgaagcc atcacggccg tcttcggccc cttcggcgtc
     7021 ccggtcaccg cccccaagac gatgaccggg cgcctctact cgggcgccgc gccggtggac
     7081 gtggtctccg ccctgctcgc cgtccgcgag gggctgatac cgcccaccac caacgtggag
     7141 cgttcggccg agtacgacat cgacctcgtg accgggcagc cgcgcaccgc cccggtgcgc
     7201 accgccctcg tgctcgcccg cggccacggc ggccacaact cggcggtcgt cgtccgggcc
     7261 gccgactgag ggcaggcgcc cacgatcccg cgcccggcgc cgtgcctccc ccttcacggc
     7321 gccgggcgcg cacgttcccg gcgcgtccgc agcgggcgcg ccgcatccga ggagaggaaa
     7381 ggacccacat gcccgagcag aagttcaccc tcgaggacct caagcggatc ctccgcgagg
     7441 gcgccggcac cgacgagggc gtcgacctcg acggagacat cctcgacacc gacttcgagg
     7501 agctgggcta cgagtcgctg gcgctgctgg agaccggcgg ccgcatcgag cgcgagtacg
     7561 gcatctccct ggacgacgac gtcttcacgg agaaccgcac cccccgctcc ctgatcgacg
     7621 ccgtcaacga gcacctcgcg gcgatcctga cgcactcgtg acggcccgcc gccgggcccc
     7681 ggggggtcct cccgggggcc cggcggcctt ccccgcccac tcgctcactc gcccactcgc
     7741 tcacgaaccc ttccggttcc ctttccgacg aaccacctgg agaacagaca tgtcgcagca
     7801 gaacaagcgg gtcgccgtgg tcaccggcgc caccagcgga atcggcctgg ccgtcgcacg
     7861 cctcctggcc cgggaccaca aggtgttcat cggggcgcgg aacgccgaga acgtcgcacg
     7921 gaccgtcaag gagctccagg gcgagggcct ggaggtcgac ggcaccaccg tggacgtccg
     7981 ctccgccgac gaggtgcgcg ccttcgtcca ggccgcggtc gaccgcttcg gcacggtgga
     8041 cgtgctggtg aacaacgccg gacgcagcgg cggcggcgtc accgccgaca tcgacgacga
     8101 gctgtgggag gacgtcgtcg acaccaacct caacagcgtc ttccggatga cccgcgaggt
     8161 gctgaacacc ggcgggatgc gcggcaagga ccgcggccgc atcatcaaca tcgcctccac
     8221 cgcgggcaag cagggcgtcg tcctcggcgc tccctactcg gcctccaagc acggtgtcgt
     8281 gggcttcacc aaggccctgg gcaacgagct ggcccccacc ggcatcaccg tcaacgccgt
     8341 ctgccccggc tacgtcgaga cgcccatggc gcagcgggtc cggcagggct acgccgccgc
     8401 gtacgacacc accgaggagg ccatcctgga gaagttccag gcgaagatcc cgctgggccg
     8461 ctactccacc ccggaggagg tcgccggcct ggtcggctac ctggcctccg acaccgccgc
     8521 ctccatcacc tcccaggcgc tgaacgtctg cggcggcctc ggcaacttct gagcgaccgg
     8581 ccccgcccgg cccgccgggc ccggacggaa ccccgggccc gcgccgggcc cgcccggaac
     8641 ccggaccccg aaccccggaa cccgaacccc ggaacccgaa ccccggaccc cgaaccccgg
     8701 accccgaaca cccgaaccga ctccgaagca gaggagcacg agcatgtccc ggtccgccct
     8761 gcgcgaggtg gagcacgaga tcaccgtctc ggccccggcc gccaccgtct accggctgat
     8821 agccgaggtg gagaactggc cgcggatctt cccgcccacc atctacgtcg accacgtcga
     8881 gaggaacgag gactccgagc gcatccgcat ctgggccacc gccaacggcg aggccaagaa
     8941 ctggacctcc cgccgcgtcc tggacccgga gaacctgcgg atcggcttcc gccaggaggt
     9001 ctccgccccg ccggtcgcct ccatgggcgg cacctgggtc gtcgaggccc tctccgaaac
     9061 cgagtccagg gtccggctgc tgcacgacta ccgcgccgtc gacgacgacc ccgagagcct
     9121 gcggtggatc gacgaggccg tcgaccgcaa ctcccgctcg gagctggccg ccctgaaggc
     9181 caacatcgag caggcgcacg ccgccgagga cctgaccttc tccttcgagg actcggtccg
     9241 catcgccggc tcggccaagg acgccttcga cttcgtcaac gaggcgggcc tgtggcccga
     9301 gcggctgccg cacgtggcca ccgtccgctt cgaggaggag acgcccggcc tgcagaccct
     9361 ggagatggac acccgcgcca aggacggctc ggtgcacacc accaagtcgt accgggtggc
     9421 cttcccgcac cacaggatcg cctacaagca ggtcacgctg ccggccctga tgaccctgca
     9481 caccggctac tggacgttcg agcaggacga ccagggcgtg accaccgcca cctcccagca
     9541 caccgtcacc ctcaacaccg cgaacatcga gcggatcctg ggcccggagg cgaccgtgga
     9601 ggacgcccgg gagtacgtcc gctcggcgct gagcaccaac agccgcgcca ccctcggcca
     9661 cgccaaggac tacgcggaag ccagggcctg accgtgccgg ggcccttcac cgaaacccag
     9721 gtgatcgtgg tcggcgcggg cccggtcggg ctgatgctcg ccggggagct gcggctcgcg
     9781 ggcgcggacg tggtggtcct ggagaggctc accgcgccca ccaccgagtc gcgggcgtcg
     9841 accctgcacg cccgcaccgt ggagatcctc gacggccgcg gcctgctgga gccgctcggc
     9901 gacgtgccga acgaccccag gggccacttc gggggcatcc cgctcgacct cacgctgccc
     9961 tcgtcccacc cgggccagtg gaaggtcccg cagacccgga tcgaggagct gctggggcgc
    10021 tgggcggcgg acctgggcgc ggacgtccgg cgcggccacg agctgaccgg actgaccgcg
    10081 tcccgggacc acgtcgaggt cgaggcggtc accgcgggcg gtgcgaccgt ccgcctgcgg
    10141 gccggatacg tcgtcggctg cgacggcgag gacagcaccg tgcgccgcct ggggggcttc
    10201 gacttccccg gcacggacgc cgtccgcgag ctgctgcgcg ccgacgtgtc cggcatcgag
    10261 atccccgacc gccgcttcca gcggctggag ggcggcctgg ccatcgccgc ccggcgcccc
    10321 gacggggtga cgcgcgtgat ggtgcacgag ttcggcgccg tgccccggcg gggacagccg
    10381 cggttcgccg acgtcgccga cgcctggaag cgggtcaccg gggaggacat cggcggcggc
    10441 accccgctgt gggtgaactc cttcggcgac gcatcgcggc aggtcaccgc ctaccgccgc
    10501 gaccgggtgc tcctcgccgg cgacgccgcg caccggcaga tgcccatcgg cggccaggcg
    10561 ctcaacctcg gcctccagga cgcggtcaac ctcggctgga agctggccgc gcaggtgcgc
    10621 ggccacgccc ccgagggcct gctcgacagc taccacgcgg agcggcaccc cgtcggccgg
    10681 cgggtcctgg acaacatcag gagccaggca ctgctgctcc tggggggccc ggaggtcgag
    10741 gccgtccgcg aggtcctggc ggaactcacc ggaggcgagg acgcgcgccg gcacctcgcc
    10801 gcgatggtct ccggcctgga cgtccgccac gacgtcgggc ccggcgacca ccccctgctc
    10861 ggccgccggc tcccgcactg ggagctggcg accggcgaag gacgcgtcac caccgcggag
    10921 ctgctgcgca ccgggcacgg cctcctgctg ctcctgccgg gcgcgcggga gcgcctggag
    10981 gagctgcgca ccgccgccgc cccctgggcg gagcgggtcc gcacggtgac cgcccgaccg
    11041 gtgcccggcg gccaggaggc gccggacacc gcggccctcc tcgtgcggcc cgacggtcat
    11101 gtcgtctggg ccgacggcga ccccgcgggc accggcgtca cggcggcgct gaaccgctgg
    11161 ttcggcgccg ggaggagggc ggacgggccg gcggcgcgga aggaccgggc gccggcgtgg
    11221 accgcgacca gagcaccagc gacgaagtgg agaacagaca tgggcaggct cacgggcaag
    11281 accgcgctcg tcaccggctc cagccggggc atgggccgcg cggccgcgct gcggctggcg
    11341 gccgacggcg cactggtcgc cgtccactac acgagcaacg aggaggccgc cgccgaggtg
    11401 gtcgagctga tcgagaagga cggcggccgg gccttcaccg tccgggccga gctgggcgtg
    11461 cccggcgacg tccacgagct cttcctcggc ctggaggagg ggctgcgcga gcgcaccggc
    11521 gggacggacc tgaacatcct ggtcaacaac gccggtgtga tgggcggggt caagccggag
    11581 gagaccaccc cggagaagtt cgaccgggtg ttcgcggtca acgccagggc cccgttcttc
    11641 ctgatccagc gcgcgctgaa gaacatgccg gacggcggac gcatcatcaa catctcctcc
    11701 ggcctcaccc gcttcgcgaa cccggacgag atcgcctacg cgatgtccaa gggagcggtg
    11761 gagcagctcg ccctgcactt cgcgaagtac ctcggcccgc gtggcatcac cgtcaacagc
    11821 gtggcgccgg gcatcacccg caacgacaac cccgtcttcc agatccccga ggcggtggag
    11881 cagatgagcg ggctgtccgc cttcggccgg gtcggcgagc ccgaggacgt ggccgacgtg
    11941 gtggccttcc tggcctccga cgacgcccgc tggatcaccg gctccttcgt ggacgccacc
    12001 ggcggcacgc tgctcggctg agccgctgct cggctgagcc gctgtccggc cgggcagcgt
    12061 gccgggggcc ggcgcccctc cacgggcgcc ggccccggcc gttcgacgag aaccctctgg
    12121 cggaacggga gcgagatgcc tgagaacaac ccctcagtcc ccgccgcggc cacggcaggc
    12181 ggcggctgga caccggccct gtgggggctg ctgctcgtac tcgcgggcaa catgctcatc
    12241 gacgccctgg aggtctccgt ggcgctggtg gcgctgcccg ccatcgggga cgacctgggc
    12301 ctggcgcccg accggctgca ctgggtcgtc agcggattcg ccgccgggtt cggcggcgta
    12361 ctgctcctgg gcggccgggt ggtcgcgctg ctgggacgcc ggccggtcta cctgaccgcg
    12421 ctcgcggtct tcgccgccgc gtccctcgcc ggcgcgctcg ccgaggaccc ggcgctgctg
    12481 atcgccaccc gcttcgtcaa gggcgtctgc gcggcgctca ccgcccccac cggcctggcg
    12541 atcatcctct cggccttccc gcagggcccg gcccgcaacc gcgcggtgtc ggtctactcc
    12601 ctgttcggcg cggccgggtt cgccacgggc ctgctgctct ccggcgccct caccgagatc
    12661 ggctggcgct ggacgttcgc cttcccggcg ccggtggcgc tgctgctgtt cgccgccgcc
    12721 gtgcgcctgg tccccggcga cccgcccgac cggagcggcg gcgggagcgg tctggcgggc
    12781 gcggccggcc tcaccgtggt gacgctgctg ctggtgcacg cgatcggctc ggtgccccgc
    12841 accggctggg gcgccccgtc caccgcgggc gcgttcgccc tcgcgggggt gctggccgtc
    12901 ctgctcgcgc gccgcgagct gaccggcccc cgccccctgg ccgatgcccg gctcctcacc
    12961 cacgcgcccc tggcccgctc ggccctcgga gccgccgcgc tcaacggctc ccacatcggg
    13021 atcctcatgc tgagcgccct gcacctgcag cggcacgcgg gcctcggccc gtggcagacc
    13081 gggctcgcct tcctgcccgc cagcgcgccg ctggtgctca gcgccctgta ctccggccgt
    13141 atggtcgccc gcctcggccc cgcgcggccg atcgccgccg gggcggccgc ggccgccctg
    13201 ggctacgcgc tctacccccg cgacccgtcc gcgccgctgg agtacgcgac cgatgtgctg
    13261 cccaccatgc tgctgatcgg ggccgccttc gtgctcgcct tcaccgccct ccacgtccag
    13321 gccacgagca cggcgccggc cggccggcag gccgaggcgg gcggcctgta ccagaccgcc
    13381 gtgcagctgg gcgccgccct gacgacggcc ctgaccgccg ccgtgtacac ggcggcgggc
    13441 cacggggcgg ccgtgctgct ggtcacggcc gtcggcgcgg cgggccttgt ggtggcgctg
    13501 ccggggctgc gccgggagac ccgcaccgac cccctgcccg ccgacctcac gaggaggcgg
    13561 acttgacggc gaacccgacg gcggaacggg ccgcgcacac ccgcgcggcg ccgaaggccc
    13621 ccgccggggc ggccgccccc gacgccgccc cggacagggg cgtacgggcc gccgccgacc
    13681 accaggaccg gatccgggaa ctcgacgcgt acgtgcgccg gctgatcggc cgtgtgctgg
    13741 gagtcgcccc gcacctcgtg gacaccgcgg gccgcccgat gaacagcctc ggcatcggct
    13801 ccatcgcggg cctggaactc cagcgccgca tggagcggga cccgcacgtc cgggtggacc
    13861 tgcggcggct gctcctggcg ggcagcgccg ccgaactcgt cgactgtccg gccgggcagc
    13921 tgcgcgggcg gcgtcacacc ggcgtggacc cggcgtgacg ccgcccgcga ccggtccggc
    13981 cgccggtggc gcgcggcccc acccggccgc cggggaggcg gagttcaccg tcgcccgcgg
    14041 ccggctggac ctgtgggtgg tgcgcagccc cgaggggcgc accgcacggg aactcgaccg
    14101 ctcggtcctg gacgacgcgg aacggcggcg ggccgacatg ttcctgcggc cggccgacgg
    14161 cctgctgtac accgcggccc acatcgcgct gcgccggctg ctcggccgct acctgggggt
    14221 ggcggccgag gaggtgaggt tcttccgcga gccctgcccc ggatgcggcg ggccgcacgg
    14281 ccggcccgcg gcggcgccgc cggacccgac ggacccggcc ccgcacttct cgctctcgca
    14341 cagcggcggc ctggccgtgg tgggggtggc ggcggtgccg gtcggggtcg acgtggagcg
    14401 gctgcccggg gaggagaccg tcgaggtgtg caccccggcg ctgcaccccg gcgagcgcgc
    14461 cgaactggcc gaggtggggg gcgaggccag aagggagctc ttcggccgcc tctggacgcg
    14521 caaggaggcc tacctcaaag ggctcggcac gggcctgggg cgtgcgccgg accgggacta
    14581 cctgggcgcc gacgtccgcc gccacccgcg cgggtggacc gtgctggacg tactgcgcac
    14641 ccccacccac accgccgcgg ccgccgtgcg gggccgcgag cccgcggtgg tggccgtccg
    14701 gccgctggcc gagacctgcc tgctcgccgg cggggaggcg cgctcggcgc gaccggagcc
    14761 ggccccggcg gcgcgctgac cgggccggcc gccgaacgcc ggccaccggg cccgcggcgg
    14821 tccggccggc cccgccggcc cgttcccgtt catccgcacc ggccgcggcg cccgcgccgc
    14881 ggccgacgac gtccgaccaa cgacccaggg agtgtggcac gaccatgtcc gaggagaacg
    14941 agacgatcca tcaccggata gaggagctgg ccgagatcaa ggagacggcg cgccggggac
    15001 cggaccccgc cgccaccgaa cgccagcacg ccagggggaa gctgacggcg cacgagcgca
    15061 tcgggctgct cctggacaag ggcaccttca ccgaggtcga gccgctgcgc cggcaccgcg
    15121 ccaccggctt cggcctggag gacaggaagc cgtacaccga cggggtggtc accggctggg
    15181 gcaccgtcca cggccggacc gtcttcgtct acgcccacga cttccggatc ttcggcgg
//
