LOCUS       NZ_FOSG01000020        10954 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces pini strain PL19, whole genome shotgun sequence.
ACCESSION   NZ_FOSG01000020
VERSION     NZ_FOSG01000020.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces pini
  ORGANISM  Streptomyces pini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900114215.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:14:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,762
            CDSs (total)                      :: 5,677
            Genes (coding)                    :: 5,560
            CDSs (with protein)               :: 5,560
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 20 of 117
            Pseudo Genes (incomplete)         :: 111 of 117
            Pseudo Genes (internal stop)      :: 1 of 117
            Pseudo Genes (multiple problems)  :: 15 of 117
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 103251
            Orig. end                         :: 114205
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOSG01000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10954
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10954
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10954
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5954](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5954)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(98..421)
                     /locus_tag="BM237_RS23500"
                     /old_locus_tag="SAMN05192584_120103"
     CDS             complement(98..421)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846517.1"
                     /locus_tag="BM237_RS23500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_120103"
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="WP_093851710.1"
                     /transl_table=11
                     /translation="MAKITYKHPNGTETIVDVAAPNTVMRGAKINNIDGIEAQCGGSAQ
                     CGTCHVYVDEANTLPLPEIHESEDEVLYGTACPRRDNSRLSCQLPVSEEIDGLVVHLPE
                     AQS"
     gene            631..2148
                     /locus_tag="BM237_RS23505"
                     /old_locus_tag="SAMN05192584_120104"
     CDS             631..2148
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /locus_tag="BM237_RS23505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_120104"
                     /product="MFS transporter"
                     /protein_id="WP_093851711.1"
                     /transl_table=11
                     /translation="MTTWTPRASGTTGRDTRADGGRGGRLGLVLFACCLGQFMNVLDAS
                     VVNVALPVIAEELGFERHTLQWVNNAYTIAVCGFLLLGGRLADLFGQRRVFLFGVTLFT
                     LASSVGGAADDPVTLVLARAFQGLGAAVMAPATLTVLGTTFTEPAARARAFGMWSAVSG
                     VAGAAGVLVGGVVTQWFSWRWVLLINVPLGLLLLAAVYWAVPGTGARRDGPGGRKGLDL
                     PGALAVTCGLMATVHGVAASGAHGWDSPRVWGTTAAGAALLGLFVLHQARFARHPLVPL
                     GVFRNRSLTAANLVAFFGIAALFSTFYFFTLVLQQVLGYSPLRTGLSYLPMSLGIALGG
                     WGIAWTVPRTGPRPVLLGGLSLSCAGLLWLSGVDAGSAYTADVLGPALLLGFGMGAVLN
                     ATTNAATAGVPARDAGLASGLLNTTRQLGSAAGLVTLAALSTARADRELAGGAALEDAL
                     ASGYGLALTGAALFTAAGLLAALAVPGRGTPVQGHSGPRALQPSSRR"
     gene            2186..3745
                     /locus_tag="BM237_RS23510"
                     /old_locus_tag="SAMN05192584_120105"
     CDS             2186..3745
                     /NRPS_PKS="Domain: Abhydrolase_1 (91-244). E-value:
                     4.9e-10. Score: 31.4. Matches aSDomain:
                     nrpspksdomains_BM237_RS23510_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018846530.1"
                     /locus_tag="BM237_RS23510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_120105"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_093851712.1"
                     /sec_met_domain="PF00561 (E-value: 1.1e-14, bitscore: 47.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATNPPVTAGADALRAFGSQRPQWTVRTPGGTDGPEDPEGTGEGS
                     GGSGTGARVEYATIEVPRDYADPEGERVTIALSRRRATDPARRRGVLLAVNGGPGGDWG
                     LGRELPGKYEGGPVHEVYDLIGFDPRGTGDSTRLYAEVTVPAAPFDSRPPDSAFEALAE
                     DMRLRELACERAGGELRRHISTRNTARDMDVIRAVLGEERISFVGYAYGAYVGAVFGSM
                     FPDRLDRSVLDSCVHPGWTWREQFLWQGDAVRRNVDRWAGWTGERDGHFGLGTGPEQVL
                     AVVEEVVARLDEQCDTPRLRTLLDGAVGNRATDRSRWEELGLLVAGLREATAAGDLDRC
                     HELLAEQGTWRPEDSEGDLRVGVLEAITLEHAWPADLEVYHQDMRDFRERFPYGYGVLR
                     AQPWVGAFRTFTAPEEPTRITRDGYPAGLVVQADGDPLDHYEGGVAMAGLLGHTLLTVE
                     DSGDHEVYALAGNPGVDAHVERYLTDGVLPPPGTTCPGTVSRPDVPADAPADVPPGVPA
                     GV"
     misc_feature    complement(2231..2242)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     aSDomain        2459..2917
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS23510_Abhydrolase_1.1"
                     /evalue="4.90E-10"
                     /label="BM237_RS23510_Abhydrolase_1.1"
                     /locus_tag="BM237_RS23510"
                     /protein_end="244"
                     /protein_start="91"
                     /score="31.4"
                     /tool="antismash"
                     /translation="LLAVNGGPGGDWGLGRELPGKYEGGPVHEVYDLIGFDPRGTGDST
                     RLYAEVTVPAAPFDSRPPDSAFEALAEDMRLRELACERAGGELRRHISTRNTARDMDVI
                     RAVLGEERISFVGYAYGAYVGAVFGSMFPDRLDRSVLDSCVHPGWTWRE"
     gene            3911..4891
                     /locus_tag="BM237_RS23515"
                     /old_locus_tag="SAMN05192584_120106"
     CDS             3911..4891
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="BM237_RS23515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_120106"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_245793973.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 5.4e-13, bitscore:
                     41.4, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRPRILLTGATGFIGSRVLRTLLGEGTADGPAASVRALGRTVPS
                     GAPDVPGLSWTRADLGDPSSLRGAARGQDVLVHLASRITGSEAECAAVNVRGTAALVEE
                     AREAGVRRIVHLSTAAVYGPGPHRGIGVGETEPAPVSAASVTRLAGEEPALAAGGVVLR
                     PNLVLGAGDRWVVPALAELLERVPARWDGGAALVSMVDAGDLARLVTALACSPGEPPAG
                     VYHAGHPRPVRLRDLMDRLASLGVLPGVFEDLPWEDCLRRLRETPGTVDERRFALLGLD
                     HWYRSDEVWRVAGCPAGPGPLARLEEAAPWYRGLLAGRRADRDGR"
     gene            complement(5001..5954)
                     /locus_tag="BM237_RS23520"
                     /old_locus_tag="SAMN05192584_120107"
     CDS             complement(5001..5954)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140400.1"
                     /locus_tag="BM237_RS23520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_120107"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_245793974.1"
                     /sec_met_domain="AfsA (E-value: 8.3e-27, bitscore: 86.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPAVPRPRLPGGLTTTVPREYVHRAALAEVFLTGWSPLDGDRCTV
                     TAQWPRGHSFFAPVPGDRGRHYDSMLMAETIRQAGTLMSHACFGVPLGHPFLMWNLNFS
                     VLPGSLAIGGSPADLRLDITCSDVSHRGNRLAGMRYTATVRVDGEVVATGGARFTCVSP
                     AVYRRLRAGRTQVSRPPAPGLPVTPGLVGRSSPFDVVLSPTGEPGRWSLRNDLSHPVLF
                     DHPVDHVPGMVLLEAARQAVHALDGTGRPLLLTSLDSTFEKYVEFDRPCWIEAEPVPSA
                     PGIPGDTVRVTAHQDGEAVFTTTVTAMSRVPALQPA"
     misc_feature    complement(6086..6106)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.75"
                     /tool="antismash"
     misc_feature    6102..6111
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 12.94"
                     /tool="antismash"
     misc_feature    complement(6104..6117)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.85"
                     /tool="antismash"
     misc_feature    complement(6105..6118)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.67"
                     /tool="antismash"
     misc_feature    complement(6106..6119)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.76"
                     /tool="antismash"
     misc_feature    complement(6118..6131)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.71"
                     /tool="antismash"
     misc_feature    6123..6140
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.49"
                     /tool="antismash"
     gene            complement(6158..6817)
                     /locus_tag="BM237_RS23525"
                     /old_locus_tag="SAMN05192584_120108"
     CDS             complement(6158..6817)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122072.1"
                     /locus_tag="BM237_RS23525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_120108"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_245793976.1"
                     /transl_table=11
                     /translation="MARQDRALRTRRAILEAAASVFEEKGYDAAKLTEILERAHVTKGA
                     LYFHFASKEELAQAVLDVQTREGTVSLAQPSKAQEFVDLGMVFAYRLTTDPLLRGSIRL
                     ALDQGAGHVPYRSRPYQEWMEICVGTLREARLNGELLSHVDPVESGELFVGAYTGINLV
                     AQILGRRDSLENSAAALYRHLMPSITLPAVLSTLDLRPERGRDVWRQANGMITSGI"
     gene            complement(6937..8094)
                     /locus_tag="BM237_RS23530"
                     /old_locus_tag="SAMN05192584_120109"
     CDS             complement(6937..8094)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM237_RS23530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05192584_120109"
                     /product="histidine kinase"
                     /protein_id="WP_093851715.1"
                     /transl_table=11
                     /translation="MGRGFAAALQDGLSRSFPPAPAAVGRTAPPAPDRPRPGGRPGAAP
                     RGPLLLPGETRAILRRFEERLPVLLGSPGTGDPLALRPALIAHARRLLGDAAGTPGTGG
                     PPTGEDRTGPVHSGTDRTGPEVRRPALDRGLAADAGVLLVECALLHVMAVSAAEDALPR
                     AAALVGALGRSLRDRPAASGRPSGGDHDEPWRECRRAARTLHDEVGTQLSVALHRLELH
                     TSRAEDPEGHLATAGDCLRSALGRTRELVTGLAEASDVPPLEEAVRSFAAQASPGTEVR
                     VTARMTGDEQGLPQALRRELLLAVRQCLHEGIRRARSTRIAVVGRVTRRWVHISVRGDG
                     EGTPGLRTVSDRIEDLGGRVAFDSAPGRGTRIDIHLPLGVRGGRP"
     gene            complement(8281..9012)
                     /locus_tag="BM237_RS23535"
                     /old_locus_tag="SAMN05192584_120110"
     CDS             complement(8281..9012)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018515159.1"
                     /locus_tag="BM237_RS23535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_120110"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_093851716.1"
                     /transl_table=11
                     /translation="MTRQERAVRTRHALIRSAAELFERHGYVQARLTEISSGAGVSPGA
                     LHFHFENKAAVADAVEHAASRALHRASRHVLGSGASALQQLTDHTHVFARMIREDVVVR
                     AGYRLNRDPARPSGPDLRQEWHSCVHGILARAADEGALRGAASRQAVAATVVAATTGLE
                     SLGKRNERWLSRGGLTRFWEVMLPAIAAPHALESVDPAGTESVVASAAPPPAYPPASPP
                     APPPALPAWKGSDGQAAENLP"
     gene            9426..10211
                     /locus_tag="BM237_RS23540"
                     /old_locus_tag="SAMN05192584_120111"
     CDS             9426..10211
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037154.1"
                     /locus_tag="BM237_RS23540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_120111"
                     /product="response regulator transcription factor"
                     /protein_id="WP_093851717.1"
                     /transl_table=11
                     /translation="MTLQTIESLDPGITGEDREAQPLSPSAGAPQAGAPVRTDREALRV
                     LVVESEPRAAQSLVQGLRRHGYLADSVATGAKALHAHHSADLVLLDLDLPDLDGLEVCR
                     SIRSSCETPVIAITARDTELDRVLGLQAGADDYMSKPYGFRELLARIQAIMRRVRPREP
                     QERIIVRGPLRIDLDNWEVRLDGKPVELTRKEFGLLRMLASRPNSVVSRRQLMAQVWDD
                     SWSRPGRTIDTHVSSLRNKLGSSSWIITVRGVGFRLGHP"
     misc_feature    10392..10403
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.68"
                     /tool="antismash"
     misc_feature    complement(10832..10841)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(10925..10934)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 ccctggcccg cgccgacgac gaccacgtcc ggcgcgccgg gctccccggc ggttcctccc
       61 gccgtgcccc gcgccgtttc ctccgtcgcc ccggccatca gctctgcgcc tccggcaggt
      121 gcaccaccag gccgtcgatc tcctcggaga ccgggagctg gcagctgagg cggctgttgt
      181 cgcggcgggg acaggcggtg ccgtacagca cctcgtcctc ggactcgtgg atctccggca
      241 gcggcagcgt gttggcctcg tcgacgtaga cgtggcaggt tccgcactgg gcggagccgc
      301 cgcactgggc ctcgatgccg tcgatgttgt tgatcttggc gccgcgcatg accgtgttgg
      361 gcgccgccac gtcgacgatg gtctcggtgc cgttgggatg cttgtaggtg atcttggcca
      421 tggcgctctc ctcggcgaaa tcggccggcc gcacatcggt aggcagcgaa cggtggcagc
      481 ggtgtctggg ggcgcactgg gagcgcgctg gagcgcttct ggagcacgac ggaggcgggg
      541 ccggagcacg gtgcggccgg ggaccgggcg cacggacggg ctcgaccggg ccgctaccgg
      601 ccgtccaccc cgccgccgca gcgtcgccgc atgaccacct ggacaccgcg cgcatcgggc
      661 accaccggca gggacaccag agcggacggc gggaggggcg gacggctcgg cctcgtgctg
      721 ttcgcctgct gcctgggaca gttcatgaac gtgctcgacg cctcggtggt gaacgtggcc
      781 ctgcccgtca tcgcggagga actgggcttc gagagacaca ccctgcagtg ggtcaacaac
      841 gcctacacca tcgccgtctg cggcttcctg ctgctcggcg gccggctcgc cgacctgttc
      901 ggccagcgcc gcgtcttcct cttcggcgtc accctgttca cactggccag cagtgtcggc
      961 ggggcggcgg acgacccggt cacgctcgtg ctcgcccgcg ccttccaggg actgggcgcg
     1021 gccgtcatgg ccccggccac cctcacggtg ctgggcacca cgttcaccga acccgccgcc
     1081 cgcgccaggg cgttcgggat gtggagcgct gtctccggag tcgccggggc ggccggggtg
     1141 ctggtcggag gcgtggtcac ccagtggttc tcctggcgct gggtactgct gatcaacgtg
     1201 cccctgggcc tgctgctgct ggccgccgtg tactgggccg tccccgggac cggggcgcgg
     1261 cgggacgggc cgggcggaag gaagggcctg gacctgccgg gcgcgctggc cgtcacctgc
     1321 ggcctgatgg ccaccgtcca cggcgtcgcc gcctccggcg cccacggctg ggactcaccg
     1381 cgggtctggg ggacgacggc ggcaggcgcg gccctgctgg gcctgttcgt gctccaccag
     1441 gcccggttcg cccggcaccc cctggtgccg ctcggcgtct tccgcaaccg gtcgctgacc
     1501 gccgccaacc tcgtcgcctt cttcggtatc gcggccctct tcagcacctt ctacttcttc
     1561 accctggtcc tccagcaggt gctcggctac agcccgctgc gcaccgggct gagctatctg
     1621 ccgatgtccc tgggcatcgc cctcggcggc tggggcatcg cctggacggt gccccgcacc
     1681 ggcccccggc cggtgctgct gggcgggctg tcgctgtcgt gcgccgggct gctgtggctg
     1741 tcgggggtgg acgccggctc cgcctacacg gccgacgtcc tcggccccgc cctgctgctg
     1801 ggcttcggca tgggcgccgt cctcaacgcg acgacgaacg ccgccaccgc cggggtgccc
     1861 gcccgcgacg ccggactggc ctcgggcctg ctgaacacca cccgccagct cggcagcgcg
     1921 gccggcctgg tgaccctggc cgccctctcc accgcccgcg cggaccgcga actcgccggc
     1981 ggggcggccc tggaggacgc gctcgcctcc ggctacggac tggccctgac cggcgccgcg
     2041 ctgttcaccg ccgcgggact gctggccgcc ctcgccgtac cgggccgggg gactccggtc
     2101 caagggcact ccggtccaag ggcgctccag ccgtcttcga gacggtgacg ggacgctggc
     2161 gccctgacga gaacggaggt ttccgatggc gaccaatccg cccgtaacgg cgggcgccga
     2221 cgcgctgcgc gcgttcggtt cccagcggcc gcagtggacg gtccgcacgc ccggcggcac
     2281 cgacggaccc gaggaccccg aaggcaccgg cgaggggagc ggggggagcg gcaccggggc
     2341 ccgggtggag tacgcgacca tcgaggtccc ccgcgactac gccgacccgg agggtgagcg
     2401 cgtcaccatc gccctcagcc gccgccgggc caccgacccc gcccgccgcc gcggcgtcct
     2461 gctggccgtc aacggcgggc ccggcgggga ctggggcctg ggccgcgagc tgccgggcaa
     2521 gtacgagggc ggccccgtcc acgaggtcta cgacctcatc ggcttcgacc cgcgcggcac
     2581 cggggactcc acccgcctgt acgccgaggt caccgtcccc gccgccccct tcgactcccg
     2641 gcccccggac tccgcgttcg aggccctggc cgaggacatg cggctgcgcg agctggcctg
     2701 cgagcgggcc ggcggcgaac tgcgccgcca catcagcacc cgcaacaccg cccgcgacat
     2761 ggacgtcatc cgcgccgtcc tcggcgagga gcggatcagc ttcgtgggct acgcctacgg
     2821 cgcgtacgtc ggcgccgtct tcggcagcat gttccccgac cgcctggacc gcagcgtgct
     2881 cgactcctgc gtccaccccg ggtggacctg gcgcgagcag ttcctgtggc agggcgacgc
     2941 cgtccggcgc aacgtcgacc ggtgggccgg gtggaccggc gagcgcgacg gtcacttcgg
     3001 cctgggcacc ggacccgagc aggtgctcgc cgtggtggag gaggtcgtcg cgcgcctgga
     3061 cgagcagtgc gacacaccgc ggttgcgcac cctcctcgac ggcgcggtgg gcaaccgcgc
     3121 gaccgaccgg agcagatggg aggaactggg cctgctggtg gcgggactgc gggaggcgac
     3181 cgccgccggc gacctggacc gctgccacga gctcctggcc gaacagggca cctggcggcc
     3241 cgaggacagc gagggcgacc tgcgcgtcgg cgttctggag gccatcaccc tggaacacgc
     3301 ctggcccgcc gacctggagg tctaccacca ggacatgcgc gacttccgcg agcggttccc
     3361 ctacggctac ggcgtgctgc gcgcccagcc gtgggtcggc gcgttccgca ccttcacggc
     3421 accggaggag cccacccgca tcacgcgcga cggctacccg gcgggcctgg tcgtccaggc
     3481 cgacggcgat ccgctggatc actacgaggg cggcgtcgcc atggccgggc tgctcggcca
     3541 caccctgctc accgtggagg actccggcga ccacgaggtc tacgccctgg ccggcaaccc
     3601 cggcgtcgac gcccatgtcg agcgctatct gaccgacggc gtcctcccgc ccccgggcac
     3661 cacctgcccg ggcacggtct cccgcccgga cgtcccggcg gatgccccgg cggacgttcc
     3721 gccgggcgtc ccggcgggcg tctgacccgc ccggggcggc gctacggtgg ggctccgttc
     3781 tcggagggag agggggaccg accgtgaagt acccgtatcc gtacccgtgt tcgcgcggtc
     3841 cggtagccgg gccgcgcgga tgggcgcggc ccggcgggag ggcccggagg gccgcaaggg
     3901 ggaccgtccg gtgagccggc cgcggatcct gctgaccgga gcgacggggt tcatcggctc
     3961 cagggtgctg cgcacgctgc tgggggaggg aacggccgac gggccggcgg cctccgtgcg
     4021 ggccctgggc cgcaccgtgc cgtccggcgc gccggacgtc cccgggctct cgtggacgcg
     4081 ggcggatctg ggcgatccct cctcgctgcg gggcgcggcc cgcgggcagg acgtactggt
     4141 ccacctcgcc tcgcggatca ccgggagcga ggcggagtgc gcggcggtca acgtgcgcgg
     4201 caccgcggcc ctcgtggagg aggcgcgcga ggccggggtg aggaggatcg tccacctgtc
     4261 caccgccgcg gtctacgggc cgggcccgca ccggggcatc ggcgtgggcg agaccgaacc
     4321 cgccccggtg tccgccgcga gcgtcacccg gctggccggg gaggagcccg ccctcgcggc
     4381 gggcggtgtc gtgctccgcc ccaacctcgt gctcggggcg ggcgaccggt gggtggtgcc
     4441 ggccctcgcc gaactgctgg agcgcgtgcc ggcccgctgg gacggcggcg ccgcgctggt
     4501 gtcgatggtg gacgccggcg acctggcccg gctggtgacc gccctggcgt gctcgcccgg
     4561 ggagccgccg gccggtgtgt accacgccgg ccacccgcgg ccggtgcgcc tgcgcgacct
     4621 catggaccgc ctggcgtcgc tgggcgtgct gcccggggtc ttcgaggacc tgccctggga
     4681 ggactgcctg cggcggctgc gggagacacc gggcacggtg gacgaacgcc ggttcgccct
     4741 gctggggctc gaccactggt accgcagcga cgaggtctgg cgggtcgcgg gctgccccgc
     4801 cggcccggga ccgctggcgc ggctggagga ggccgcgccc tggtaccgcg gcctgctcgc
     4861 cggacggcgg gcggaccggg acggccgctg aacaggcacc gcgacgagcg ctgcacggcc
     4921 ggtccggccg gcccctccgg cagggggccg gccggaccgg ccgggaccgc gtgcggggcg
     4981 gtgcctccgg gaggggccgg tcaggcgggc tgaagggccg gcacgcgcga catggccgtg
     5041 accgtggtgg tgaagaccgc ctcaccgtcc tggtgcgcgg tgacgcggac cgtgtcgccg
     5101 gggatgccgg gggccgacgg cacgggctcg gcctcgatcc agcacggccg gtcgaattcc
     5161 acgtacttct cgaaggtgct gtccaggctg gtgagcagca gcggacgccc cgtgccgtcg
     5221 agcgcgtgga ccgcctggcg ggcggcctcc agcaggacca tgccgggcac gtggtccacc
     5281 gggtggtcga agaggacggg gtggctgaga tcgttcctca gcgaccagcg ccccggctcg
     5341 ccggtggggg agaggacgac gtcgaagggc gaggagcggc ccacgaggcc gggggtgacg
     5401 ggaaggccgg gggccggggg acgggacacc tgcgtgcggc cggcgcgcag tctgcggtag
     5461 acggccgggg agacgcaggt gaaacgggcc ccgccggtgg ccacgacctc gccgtcgacc
     5521 cggacggtgg cggtgtaccg cattccggcc agccggtttc cgcggtggct gacgtccgag
     5581 caggtgatgt ccagccggag gtccgcgggg gaccccccga tcgcgagact gccgggcagg
     5641 accgagaagt tgaggttcca catcaggaag gggtggccga ggggcacgcc gaagcaggcg
     5701 tgcgacatca gcgttccggc ctggcggatc gtctcggcca tcagcatgga gtcgtagtgg
     5761 cgtccgcggt ccccggggac cggcgcgaag aagctgtgcc cccggggcca ctgggcggtc
     5821 acggtgcagc ggtcgccgtc gagcggtgac cagccggtca ggaagacctc ggccagtgcg
     5881 gcccggtgga cgtactcccg ggggaccgtc gtggtcagcc ccccggggag gcgggggcga
     5941 ggaacggcgg gcacgaaagg tgcaccggaa ccggcggagc cggcgggacc ggcggaaccg
     6001 gcgacgaagg catcgatcat gagatccccc aagcactgct tacgaccgga tgggcgggcg
     6061 tcttgctgtg tcgtgcttcg agggaaagat acctggtgct cggttttttt ctaaggagtt
     6121 ctttgtcaaa tcttggtgaa tcgctgacat tgggggatca gattccggaa gtgatcattc
     6181 cattggcctg tcgccacacg tcccgtcccc gctccgggcg gaggtcgagc gtggacagga
     6241 cggccggcag cgtgatgctc ggcatgagat ggcggtagag ggccgcggcg gagttctcca
     6301 gggagtcgcg gcggcccagt atctgcgcca ccaggttgat gccggtgtag gcgccgacga
     6361 acagctcgcc gctctccacc gggtcgacgt gcgacaggag ttcgccgttc aaccgcgcct
     6421 cccgcagcgt gccgacgcag atctccatcc actcctggta ggggcggctg cggtacggca
     6481 cgtgcccggc tccctggtcg agggcgagcc tgatgctgcc ccgcagcagg gggtcggtgg
     6541 tgaggcggta ggcgaagacc atcccgaggt cgacgaactc ctgtgccttg ctcggctggg
     6601 ccagcgagac cgtgccctcc ctggtctgca cgtccaggac ggcctgcgcc agctcctcct
     6661 tggaggcgaa atggaagtac agggcgccct tggtgacatg cgcgcgttcg aggatctccg
     6721 tcagcttcgc ggcgtcgtat cccttctcct cgaagacgga cgcggcggcc tccaggatcg
     6781 ctcggcgtgt ccggagggca cggtcctgtc tggccatatg ggcatccgtc ctctggtgag
     6841 actctgttcg ccgtacgcgg tcaagtatag accgtccggt atgtttccgc cggggtggcg
     6901 gcaccgggcg gcggccggaa gcccgcgcgg gcgccgtcag gggcggccgc ccctgacgcc
     6961 caggggcagg tggatgtcga tccgcgtgcc ccggccgggc gcgctgtcga aggccacccg
     7021 gccgccgagg tcctcgatcc ggtcggagac ggtgcgcagg ccgggcgtgc cctccccgtc
     7081 gccccggacg ctgatgtgga cccagcggcg ggtcaccctg ccgacgaccg cgatccgggt
     7141 ggaccgggca cggcggatgc cctcgtgcag gcactgccgc acggcgagga ggagttcgcg
     7201 ccgcagggcc tgcggcaggc cctgctcgtc accggtcatg cgggcggtga cgcggacctc
     7261 cgtgcccggt gaggcctgcg cggcgaagga gcgcaccgcc tcctccagcg gtggtacgtc
     7321 gctcgcctcg gccaggccgg tcacgagctc ccgggtgcgg ccgagcgccg agcgcaggca
     7381 gtccccggcg gtggccaggt gcccttcggg gtcctcggcc cgcgaggtgt gcagttccag
     7441 ccggtgcagt gccacggaga gctgggtgcc gacctcgtcg tgcagggtgc gggcggcccg
     7501 gcggcactcg cgccacggct cgtcgtgatc ccctccggac ggccgtccgg acgccgccgg
     7561 ccgatcccgc agcgaccggc ccagcgcgcc gacgagcgcg gccgcgcgag gcagtgcgtc
     7621 ctcggctgcc gagaccgcca tgacgtgcag cagcgcgcac tcgaccagca ggacgcccgc
     7681 gtcggcggcc agcccccggt ccagggcggg gcggcggacc tccgggccgg tgcggtccgt
     7741 tccggagtgc accggcccgg tgcggtcctc gccggtgggg ggccctccgg tgccgggggt
     7801 ccccgccgcg tcgccgagca gccgccgggc gtgggcgatc agggcggggc gcagggcgag
     7861 cgggtcgccg gttcccgggg agccgagcag gacgggcagc cgttcctcga agcggcgcag
     7921 gatcgcccgt gtctcaccgg gcagcagcag ggggccccgg ggcgccgcgc cgggccgtcc
     7981 gccggggcgc gggcggtccg gcgccggcgg cgcggtcctg ccgaccgccg ccggcgccgg
     8041 agggaaggac ctgctgaggc cgtcctgcag ggcggcggcg aagccgcggc ccaccagggc
     8101 ctccagaagg tctgtcccgg agccgcttcc gaatggagcg tcagtcctca cgtcgtcccc
     8161 cgatcgactg tgccgcgggc cgtcacgcac cggtgcggtg gcggacggag cggccaccgc
     8221 ctctcgcgtt cgcccgagaa gcaggacatg tggaagctaa caaaccgggc tccctgtttg
     8281 tcaaggaagg ttttcggccg cctgtccgtc gctccccttc caggccggaa gcgcgggagg
     8341 aggggcgggg ggagaggccg ggggatacgc gggaggaggt gcggcggagg ccaccacgga
     8401 ctccgtcccc gcggggtcca ccgactccag ggcgtgcggg gccgcgatgg ccgggagcat
     8461 cacctcccag aagcgggtga gcccgccgcg cgacagccac cgctcgttcc tcttcccgag
     8521 ggattccagc ccggtggtcg cggcgacgac cgtggcggcc acggcctggc gggacgcggc
     8581 cccccgcagg gcgccctcgt cggccgcccg ggccaggatg ccgtggacgc aggagtgcca
     8641 ctcctggcgc aggtcgggtc cggagggccg ggcggggtcg cggttgagcc ggtagccggc
     8701 gcgcaccacc acgtcctcgc ggatcatcct ggcgaagacg tgggtgtgat cggtgagttg
     8761 ctgcagggcg ctggcgccgg accccaggac gtgccgggag gcccggtgga gggcccgcga
     8821 cgccgcgtgc tcgacggcgt cggccacggc ggccttgttc tcgaagtgga agtgcagggc
     8881 gccggggctc acgcccgcgc ccgagctgat ctcggtcagg cgggcctgga cgtaaccgtg
     8941 ccgctcgaac agctcggccg cggaccggat gagcgcgtgg cgggtgcgta cggcccgttc
     9001 ctgtctggtc acgagcacct ccgggggagg tgagggcgtc gtgccgaccg cccgtggcgg
     9061 aggacgggga cggggagcac ccgagcgggg gaagttggag gctccatcat ggcaacaata
     9121 aaaccaactg gatggtttac atgccagtca ctcacgtgaa aacggccggt cccttccctg
     9181 tgcggacccg tgtgctctgg cctgcggtaa tggctccccg gatatgccgg aaggcctcat
     9241 tcccctcagc ttcgcctgtg acctgcggcg acggaaggtc ggtagggtcc cgtcggggag
     9301 gtccggcgct cgcgcggagg cggtgcggga cttgagggga agttgacaaa ccagcgaccc
     9361 ggtttgtaat gtccggcgcg acggtccact cgtgatgcgc caaccgcctc gagcaggagc
     9421 agcagatgac actgcagacc atcgaatccc tggaccccgg gataaccggg gaagatcgag
     9481 aagcgcagcc gctgtcaccc tcggcgggcg caccgcaggc cggggcaccc gtccgcaccg
     9541 accgggaggc gctgcgggtg cttgtggtgg agagtgaacc gcgagccgcc cagtccctgg
     9601 tgcaggggct gcggagacac ggctacctgg cggacagcgt ggccaccggg gccaaggccc
     9661 tgcacgccca tcacagtgcc gacctggttc tgctcgacct cgacctgccg gacctcgacg
     9721 gtctggaggt gtgccggagc atccgctcct cctgcgagac gccggtcatc gccatcacgg
     9781 cgcgggacac cgaactcgac agggtgctgg gactgcaggc cggcgcggac gactacatgt
     9841 ccaagcccta cggcttccgg gagctgctcg cccggatcca ggcgatcatg cgcagagtca
     9901 ggccccggga accgcaggag cggatcatcg tccgggggcc gctccgtatc gacctggaca
     9961 actgggaggt ccggctcgac gggaagcccg tcgagctgac ccgcaaggag ttcgggctcc
    10021 tgcggatgct cgcctcgcgc cccaacagcg tcgtctcccg gcggcagctg atggcccagg
    10081 tgtgggacga ctcgtggtcc cggccgggcc gcaccatcga cacgcacgtc agcagtctgc
    10141 gcaacaagct cggttccagc agctggatca tcacggtccg cggggtcggg ttccggctgg
    10201 gccacccctg acggccgttc gcccggacca ccggccccgg cgggccgccc gtccgggaag
    10261 cccgacggac gaccccggcc cggggcgtcc gacaccggcg cgtccactcc gcgaggcgcg
    10321 cgggaagcag cattccggac gaggacgaag gagaactcat cgtgcgcaag gtgctcatcg
    10381 ccaatcgcgg tgaaatcgct gtccgggtcg cccgggcctg ccgggacgcc gggatcgcga
    10441 gcgtggccgt ctacgccgac ccggaccggg acgcgctcca cgtgcgggcg gcggacgagg
    10501 ccttcgcgct cggcggtgac acccccgcca ccagctacct ggacatggcc aaggtcctca
    10561 aggccgccgc cgactccggc gccgacgcgg tccaccccgg ctacggcttc ctgtcggaga
    10621 acgccgactt cgcccaggcc gtcctcgacg ccggactgac ctggatcggc cccccgccgc
    10681 aggccatccg cgacctgggc gacaaggtcg ccgcccgcca catcgcccag cgcgccggcg
    10741 cccccctggt ggccggcacc cccgacccgg tggccggcgc ggacgaggtc gtcgccttcg
    10801 ccaccgaaca cggcctgccc atcgccatca aggccgcctt cggcggcggc ggccgcggcc
    10861 tgaaggtcgc ccgcaccctt caggaggtcc ccgagctgta cgactccgcc gtccgcgagg
    10921 cggtggccgc cttcggccgc ggcgagtgct tcgt
//
