LOCUS       NZ_FOSG01000010        11273 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces pini strain PL19, whole genome shotgun sequence.
ACCESSION   NZ_FOSG01000010
VERSION     NZ_FOSG01000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces pini
  ORGANISM  Streptomyces pini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900114215.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:14:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,762
            CDSs (total)                      :: 5,677
            Genes (coding)                    :: 5,560
            CDSs (with protein)               :: 5,560
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 20 of 117
            Pseudo Genes (incomplete)         :: 111 of 117
            Pseudo Genes (internal stop)      :: 1 of 117
            Pseudo Genes (multiple problems)  :: 15 of 117
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 134998
            Orig. end                         :: 146271
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOSG01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11273
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11273
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11273
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6273](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6273)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(572..590)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.85"
                     /tool="antismash"
     gene            complement(1185..1634)
                     /locus_tag="BM237_RS16095"
                     /old_locus_tag="SAMN05192584_110112"
     CDS             complement(1185..1634)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM237_RS16095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05192584_110112"
                     /product="hypothetical protein"
                     /protein_id="WP_093850355.1"
                     /transl_table=11
                     /translation="MRSATTIRRAALVAAAMASIGLINAPSASANWSSYISSWTDGEES
                     RRWEDQGTYSQVQFRDCFAQNAGSSNQKVTLTERVDISFSPDKVLDSKTFSNCFKGSSY
                     WSNGEWTDVPSGTNTLYFRADKIGQGGSCCLLFVSEVYVDTSKAD"
     misc_feature    1931..1945
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.66"
                     /tool="antismash"
     misc_feature    2052..2067
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.24"
                     /tool="antismash"
     misc_feature    2053..2068
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.71"
                     /tool="antismash"
     gene            <2237..2395
                     /locus_tag="BM237_RS16100"
                     /old_locus_tag="SAMN05192584_110113"
                     /pseudo=""
     CDS             <2237..2395
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006346068.1"
                     /locus_tag="BM237_RS16100"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_110113"
                     /product="DUF397 domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="RSSHSGTSGGDCVEVAAGPEAVYVRDSKTAGGPVLRVGRDGWAAF
                     VALAAGR"
     gene            complement(2462..3622)
                     /locus_tag="BM237_RS30320"
                     /old_locus_tag="SAMN05192584_110114"
     CDS             complement(2462..3622)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM237_RS30320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05192584_110114"
                     /product="hypothetical protein"
                     /protein_id="WP_093850357.1"
                     /transl_table=11
                     /translation="MRFTLRRKQPLEEESGACDRGFGDLDEGIGRISREIAEIRTTLAE
                     AAEKPPSQAMSGEFHRLRRAVSDGTRILQEENQTQLRRQEEILGRLGELRLEITGGRTG
                     AHPPPVLGSPGADEPGEAGDGTAETAGAQPPAGLLAPSDEAPGTQDSRRQEERMERERD
                     GVPEPPDGAAPSGEAGETPGERVRTVKKAAEQTLRNRSEDSRGRTDRTAAPPAADGRRL
                     HGVHADGEDGQRPGRRDDDRGTPPDPLAEHAELLLNAAGVSRAELVCHRDTWEFLLVQA
                     ARHPRFRVPGTVEEDAEHGLVWTHLSGPSLIAALISLWKTREKASEEVDADWAMAAVLY
                     DRIRDRLTEAGPGDGRRTVRIVLDDGDGPEGTAAAAEPSVEEAEAA"
     gene            3928..4227
                     /locus_tag="BM237_RS16110"
                     /old_locus_tag="SAMN05192584_110115"
     CDS             3928..4227
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152927.1"
                     /locus_tag="BM237_RS16110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_110115"
                     /product="NIPSNAP family protein"
                     /protein_id="WP_093850358.1"
                     /transl_table=11
                     /translation="MAKTTQLRTYTVRDGMLDEWVERWREEIVPLRLALGFTIDGAWVD
                     RERNRFVWLISYDGPETFEERNAVYWASPRRKAMGLDPDDYLAHTDDRTVERVR"
     gene            4421..4744
                     /locus_tag="BM237_RS16115"
                     /old_locus_tag="SAMN05192584_110116"
     CDS             4421..4744
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040183.3"
                     /locus_tag="BM237_RS16115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_110116"
                     /product="Scr1 family TA system antitoxin-like
                     transcriptional regulator"
                     /protein_id="WP_175541031.1"
                     /transl_table=11
                     /translation="MRDTRNGHAVRVKEDSGDPPGVWSAYGRLLQLMPAGPGFHTGLNG
                     PFVVLETKEHRHIGYVESQEVGCVVRDPAEVSAFGPRYGKLRSQALNVMESARLIGRLL
                     GET"
     gene            4741..4953
                     /locus_tag="BM237_RS16120"
                     /old_locus_tag="SAMN05192584_110117"
     CDS             4741..4953
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469279.1"
                     /locus_tag="BM237_RS16120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_110117"
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_093850359.1"
                     /transl_table=11
                     /translation="MSMEQVTRNVGEPHWFKSSYSGSGGGDCVEVAAGPEAVYVRDSKA
                     VGDGPILRVGRDEWAVFLMLAAERG"
     gene            complement(5001..6273)
                     /locus_tag="BM237_RS16125"
                     /old_locus_tag="SAMN05192584_110119"
                     /pseudo=""
     CDS             complement(5001..6273)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175675.1"
                     /locus_tag="BM237_RS16125"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_110119"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 6.9e-89, bitscore: 291.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARLGTGIGWRPGIAEAIEALPDVGWVEVVAENVCPDHLPPALVR
                     LRERGTTVVPHGVGLGLGGAQRPDPARLAALAHRAEALEAPLVTEHIAFVRAGGPGTEG
                     PVMEAGHLLPVPRTRDALEVLCENVMIAQDALPVPLALENIAALLPWPGEELGEAEFLT
                     ELVDRTGVGLLIDVANLHTNRVNRGEDPPAVLDGLPLDALAYVHVAGGVERDGVWHDSH
                     AHPVPEPVLELLAELCARTDPPGVLLERDDDFPPDEELAAELAAVRGVFEGAARNAGGP
                     RA"
     gene            6491..8221
                     /locus_tag="BM237_RS16130"
                     /old_locus_tag="SAMN05192584_110120"
     CDS             6491..8221
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358873.1"
                     /locus_tag="BM237_RS16130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_110120"
                     /product="BCCT family transporter"
                     /protein_id="WP_175541032.1"
                     /transl_table=11
                     /translation="MLEKLQRLLGLRTDPVIFFTSAGLTVLFVVAAVSLTEQVDRIFAT
                     ASDWLLTQLGWFYILGVTTFLVYLLWIAVSRYGHIRLGAQDDRPEYSTRTWFAMLFAAG
                     IGTILMFWGVAEPISHFAEPPQQGVEPESTEAARMAMAYSLYHFGLHTWSIFCLPSLTF
                     AYFVYVRGMPFRVSSVLYPLLGDRVYGTAGRIVDIAAVMGTLFGVAVSIGLGTMQINSG
                     LARLFGLPESSLVQVLLITVITAIATTSVVYGLDKGIRRLSNINIVMAVGLMLFVLATG
                     ATLFLAKGIIENVGVYLSSLVSLAFWNDTFADTGWQGTWTVFYWAWTITWSPFVGIFIA
                     RISRGRTIREFVLGVLALPTAFTIVWFSVFGLSAIDIEMNGAGGLVRNVVEEGDIPGAL
                     FTFLENYPLTTFVSALSVLIVVIFFTTSSDSASLVVDMLSAGQREEGGPTRQRVFWAIT
                     EGVVAGTLIAATGKSGLDALAEVITVLGLPFFVLAFLMMYSLVRALRTDFPELTGPPAA
                     LRRRMERERRTREWGAGKRDGEDGKGGEGETSEGAGGAEGAEGAEGAEGTGGEASESGR
                     "
     gene            complement(8228..8983)
                     /locus_tag="BM237_RS16135"
                     /old_locus_tag="SAMN05192584_110121"
     CDS             complement(8228..8983)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984734.1"
                     /locus_tag="BM237_RS16135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_110121"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_093850360.1"
                     /transl_table=11
                     /translation="MSTSDAPSTAPAAREAADRDAAPQYVLPLVVRIERSAPPGPPART
                     DALQTAARAVLVMLSDERATDPGGDWHRAVRDWQDGRIRKVVRRARGAEWRRAGALPGA
                     TVTGGGEHPAEVRVFPPVPLDGWPRDLARLQVSGTELEDPMPPAAPEPGTPVLWLNPEL
                     EMSAGKAMAQAGHGAQLAWLALDGAARAAWRAAGFPLAVRTAPRERWERLTESGLPVVR
                     DAGFTEIAPGSATVVAEHPALPVRQGGGD"
     gene            9073..9282
                     /locus_tag="BM237_RS16140"
                     /old_locus_tag="SAMN05192584_110122"
     CDS             9073..9282
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012999907.1"
                     /locus_tag="BM237_RS16140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_110122"
                     /product="hypothetical protein"
                     /protein_id="WP_093850361.1"
                     /transl_table=11
                     /translation="MEFVSGWTAPETTAVRDAGAVPAPDGTGREERAVCVQCGEPTDYP
                     ASTRGATLCPVCEWHEAERVACSC"
     gene            complement(9335..10414)
                     /locus_tag="BM237_RS16145"
                     /old_locus_tag="SAMN05192584_110123"
     CDS             complement(9335..10414)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /locus_tag="BM237_RS16145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_110123"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_175541033.1"
                     /transl_table=11
                     /translation="MTVSVRRTARPLTALALVAAAAAGCAGPGGDTTRAGASPAAPSSP
                     AASEEGSEKDGFSASPRSSVSRPDGDDQDVSPSDRYATLPASISGRVPVFRHGPRAGAP
                     QATDDESGKPEEKPAEEKPAEDDENREKRKKAEKSGDRGDKVVALTFDAALPAEDRERA
                     EEEGERHDNPGLIAALRREKVPATVFMSGLWAQTYKHQARSIGRDDLFEVGNLSHSHRA
                     FTDDCRDLPALAPGEQLADVRKGRKAIRAAGVKNLTPYFRFPGGCFDDRSRRTIAPAKV
                     TAVAGDVVAPDAGEKDPEKVAERVLKDIRPGSVVVLRCDREKAPATEKAVRRIVPELKE
                     RGYRVVHVSEMIASAMGRD"
ORIGIN
        1 cccagccaca tgttcacggc gatggacgcc tccggcttcg cgtcacaccc gaacttgggc
       61 gtcccgtcga cgatggtcgc cgccacggcg tggctcttcc acgcgagtga cagcgggatg
      121 taggcggtcg tgggcgaggg atccggttcg aaccggccgt agacgatgga gtcccgcacc
      181 gtccgcggaa cgtcctccac cacgccgtag gccttcagct tcgtgtaggt gtccctcacc
      241 tcggcggaca cggccgcgat gtcgccgcgg gcctgttcgg gcatgcagac ttccggagcc
      301 ctgccggagc acaccaccgc gacccggccc gcgttgaggg acggattgaa gttccagtcc
      361 tcgacgatgt tgtaggcccc ggccgtggag gccaccacca gcgccgcgcc gatcccggcg
      421 cgcaccgcca ggtgcccgcg gcggatccag accagtccca ccgccgccgc cacgcccgcc
      481 gtgggcagga actgggcggc catggacgcg aaggtcgcgg tctcgccgaa gccgagctgg
      541 gcggagtact cgcccatcat gtgccgcagc cacgggggat tcatcgagtg ggggaacgcc
      601 acgacgacga acaccaggca catcagcgcc ggcgcggcga tgaggggctt gacccagcgg
      661 ccgatgctga acccgatcag ggcgtgcgcg cagcacagcg ccatgcccag cagcagcggg
      721 ggaagactgt cccaggtggg ccaggtgggg gtctggacga acgccatcac cacaggggtg
      781 atgagcatca gccagcccag ggcgactatg ggggccagcg cgagtgtgat gatctgccat
      841 gtgggccggg aggcggccat ctgccacaca ccggcctcct tcagccgggc gccctgccac
      901 gcggcggcgg ccgagaccac cgcgtagctg accgcgtaca tcaactcgat cggctgctgc
      961 accagcgtcg gcgcgtacgg gtagggccag aaggcgatct gctcactggt cccggaggtg
     1021 tagtacagga ccacgagagc gagggccagc ggcagggccg gcaacgcgct cgacgtcctg
     1081 acaagggtgc gcagtcgcac tcgttctcca gagagtgggg agtgaaggag ccggcgcgtg
     1141 agcaccaccc acgcgcctac tccgccgagg tgtccggcct tcggtcagtc ggccttcgag
     1201 gtgtccacgt acacctcgga gacgaacagc aggcagcacg agccgccctg accgatcttg
     1261 tcggccctga agtacagcgt gttcgtgccg gacgggacgt ccgtccactc gccgttgctc
     1321 cagtagctgc tgcccttgaa gcagttggag aacgtcttgg agtccaggac cttgtccggg
     1381 gagaagctga tgtccacccg ctccgtcagg gtgaccttct ggttggagct gcccgcgttc
     1441 tgggcgaagc agtcccggaa ctgtacctgc gagtaggtgc cctggtcctc ccagcgccgg
     1501 gactcctcgc cgtcggtcca gctgctgatg tagctggacc agttcgcgga ggccgagggt
     1561 gcgttgatca ggcctatgga cgccatggcc gcggcgacca gtgcggcacg gcgtatggtg
     1621 gtagcggaac gcatatctgt tgctcctagc caggttacgg atgatgcggt ctggaggcgg
     1681 gcacgcctcc ctgcccctcc gggccttgcc gggcttggag tggctctaaa gatcacttac
     1741 gttcatcacg ctgtcaactc gtttactttt taaggcagtt gacaaagcgg ccactgctca
     1801 cttccttcga atgggcgccc cgatgagtgc cgcgcgtcgt gaagtcgcag gtcacaggcc
     1861 tgaagtcgat gtccgagatg ttcgtaccgg ccgcgaccgg atgaattcgg caggggtttc
     1921 cgacttcggg ggaattcacg ttccgatgcc ggaaacctat ggctcgcgtc ggctttgtca
     1981 agcggttctg ccggtcggcg tgttcgtggc ggtcggcggc tttgcggggt gttctgttcg
     2041 acggtactca gtgtgacggt tgtctcacag gaccgtcagg ggcggggcgg ccggcgccct
     2101 gccgcgcctc gcgtgcccgg ccgggcctcc gtcgggcggg tggcgcccgg cgcccgcagc
     2161 ggcacctccg cccagacggt cttggtgaac gcgtcccgca acctcgcacc cccagcgccg
     2221 ggcgtacacc gcgaccagga gcagccacag cggaacgagc ggcggggact gcgtggaggt
     2281 ggccgccggg ccggaggccg tgtacgtgcg ggactccaag acggccggcg ggcccgtgct
     2341 gcgggtcggc cgcgacgggt gggccgcgtt cgtcgcgctc gcggccgggc gctgagcccg
     2401 gtcccgcacc gctgctgcgg cccggacgcc gtccgggccg cagcggtacg agcgggcggg
     2461 gtcaggcggc ctccgcctcc tccacggagg gctcggcggc cgccgccgtc ccctccggcc
     2521 cgtccccgtc gtccaggacg atgcggacgg tacggcggcc gtcgccgggc ccggcctcgg
     2581 tcagccggtc gcggatgcgg tcgtagagta cggcggccat cgcccagtcc gcgtccacct
     2641 cctcgctcgc cttctcgcgg gtcttccaga ggctgatcag ggcggcgatg agggacggcc
     2701 cggacaggtg cgtccacacc aggccgtgtt cggcgtcctc ctcgaccgtt ccggggacgc
     2761 ggaagcgcgg gtgccgcgcg gcctgtacga gcaggaactc ccaggtgtcg cggtggcaga
     2821 ccagttcggc gcgcgacacc ccggcggcgt tcagcagcag ttccgcgtgc tcggccagcg
     2881 ggtcgggcgg cgtcccccgg tcgtcgtccc gccgccccgg ccgctgtccg tcttcgccgt
     2941 ccgcgtggac gccgtggagc cgccggccgt ccgccgccgg gggcgcggct gtgcgatcag
     3001 tacggccgcg ggagtcctcg gagcggttcc ggagggtctg ctcggcggcc ttcttcaccg
     3061 tcctcacgcg ctctcccggc gtctccccgg cctccccgga gggagccgcc ccgtccgggg
     3121 gctccggtac gccgtcccgc tcccgctcca tgcgctcctc ctgccgtcgg ctgtcctgtg
     3181 tcccgggggc ttcgtccgat ggggccaaca gccccgccgg cggctgggcc ccggccgtct
     3241 ccgccgttcc gtcccccgcc tcgccgggct catcggcgcc gggggagccc agcacgggcg
     3301 gcggatgtgc cccggtacgg ccgccggtga tctccaggcg cagttcgccc agacggccga
     3361 ggatctcctc ctgccgccgt agctgcgtct ggttctcctc ctggaggatc cgggttccgt
     3421 cggagacggc cctccggagt ctgtggaact ctccgctcat cgcctgcgac ggcggctttt
     3481 ccgccgcctc ggcgagcgtg gtgcggatct ccgcgatctc gcgactgatc cggccgatcc
     3541 cctcgtcgag atcgccgaac cccctgtcgc aggcgccgga ctcctcttcg agcggctgtt
     3601 tccgcctcag cgtgaaccgc acgcaacccc cttgtcgctt tgagtgacga gccgatcctt
     3661 tccccgggcc cgatcgggcg acgcaggcgt tttccggcca agacgggtcc gtgccaccgg
     3721 tgtcggcgga acggacggcc ccacgctgtg cggttacttc ccagcacccc cgaaggggtg
     3781 atggcctgga cgtatgtgcc ccgccccctt cctcccggtc ccgcgaagcg gaaggcggcg
     3841 ggcggcggac gatgacgcga gtccgcgaaa ccgggccgcc cctgaccatg cgcgggccgc
     3901 ggccctcgac cggaggaggc gcccgccttg gcgaagacca cacaactgcg tacgtacacc
     3961 gtccgggacg gaatgctgga cgagtgggtg gaacggtggc gcgaggagat cgtcccgctg
     4021 cgcctggcac tgggcttcac gatcgacggg gcctgggtgg accgggaacg caaccggttc
     4081 gtctggctga tctcctacga cggtcccgag acgttcgagg aacgcaacgc cgtgtactgg
     4141 gcctcgcccc ggcgtaaggc catgggcctc gacccggacg actacctcgc ccacaccgac
     4201 gaccggacgg tcgagcgggt ccgctgaccg cacgggccgc cggtggccgg gcctgcgccg
     4261 acccgtacaa ccggaccttg tacgggcgtt gtacgggtcc cgggccgtgc cccggcccgc
     4321 gcgggccgcg ttggacatgg ccccggcggt gacggacggc ggaagccgcc gggggctcgg
     4381 accagcggga cggactcggg gacgaagggg cgtgagggcg gtgcgggaca cgcggaacgg
     4441 tcacgcggta cgggtgaagg aggacagcgg cgacccgccg ggggtgtggt cggcgtatgg
     4501 caggctgctc cagctcatgc ctgccgggcc ggggttccac accgggctga acggtccgtt
     4561 cgtggttctg gagacgaagg agcaccggca catcggctac gtcgaatccc aggaagtcgg
     4621 gtgcgtcgta cgtgatcccg cagaggtcag cgccttcgga ccgcggtatg gcaagctgcg
     4681 atcgcaggcc ctgaacgtca tggagtctgc gcggctcatc ggacggctgc tgggagagac
     4741 atgagcatgg agcaggtgac caggaacgtg ggcgagccgc actggttcaa gagcagctac
     4801 agcggatcgg gcggcgggga ctgcgtggag gtggccgccg ggccggaggc cgtgtacgtg
     4861 cgggactcca aggccgtggg cgacgggccc atactccggg tcggccgaga cgagtgggcc
     4921 gtgttcctga tgctcgcggc ggagcggggc tgagcaaagc cgccgctccg gccgcgtgcg
     4981 cgcggggccc ggtacggccg ctacgctccc ggccgcgtgc ggcgcaggag gcgggagagg
     5041 agaccggccg ggccccaccc ccggggggcc cggccggagc gttcgcggta ccagcggcgc
     5101 agacggcgcc gctgctgccg ggtcagggcg gagtcggact ccaggaggtg cgtgacgaag
     5161 cgcatggcgt ccgggcggta tccgccggtc atggggcggc cgcgggcgta ggcggcgaac
     5221 gccgggcggt agcgctcgcc caggatgcgc ggcagctcgg gcgcgatccc ggcgatgacc
     5281 gcggcccgct tggcgaccag cgcccggcgc tggacctcca gtcgcgcggg gtcgaagccg
     5341 gcgggtgcgg ggccgccggc gaccagggcg cccagcaggg cggcctgggc ggcggccagc
     5401 cggcggcggg ccgcgtcggt gtcaggcacg gggacctccc gcgttccgcg ccgcgccctc
     5461 gaacacgccc cgtacggccg ccagttcggc ggccagctcc tcgtccgggg ggaagtcgtc
     5521 gtcgcgttcc agcagcacgc ccggcggatc ggtgcgcgcg cacagctcgg ccagcagctc
     5581 cagcaccggc tccggcaccg ggtgggcgtg gctgtcgtgc cacaccccgt cgcgctccac
     5641 gccgcccgcc acgtgcacgt acgccagcgc gtccagcggc aggccgtcca ggacggccgg
     5701 cgggtcctcg ccgcggttga cgcggttggt gtggaggttg gcgacgtcga tcagcaggcc
     5761 gacgccggtg cggtccacca gctcggtcag gaactccgcc tcgcccagct cctcgccggg
     5821 ccaggggagc agggccgcga tgttctccag cgccagcggc acgggcagcg cgtcctgggc
     5881 gatcatgacg ttctcgcaga gcacctccag cgcgtcgcgg gtgcgcggca ccggcagcag
     5941 gtgcccggcc tccatcaccg gcccctcggt ccccggcccc ccggcccgga cgaacgcgat
     6001 gtgctcggtg accagcggcg cctccagcgc ctcggcccgg tgcgccagcg ccgccagccg
     6061 ggcggggtcg ggccgctgcg cgcccccgag cccgagcccc accccgtgcg gcaccacggt
     6121 ggtgccgcgc tcgcgcagcc gtaccagcgc cggcggaagg tggtcggggc agacgttctc
     6181 cgcgaccacc tccacccagc cgacgtccgg cagcgcctcg atcgcctccg cgattcccgg
     6241 ccgccagccg atccccgtac ccagtcgtgc cacgcccgcc cccctccccg gtccctcccc
     6301 ggttcctccc cgtgtggggt tgtcatctcc gggcagaggg atcaccaatc ccgcgcggcg
     6361 gagttcagag cttcacctga gctttcgccg gggccccacc ccggtccgtt cgcggccgcc
     6421 gcccgtgtgt cgcgcccgca tcggaccgca aaaacgacga aaatcggacc agtcctccag
     6481 ggagaggtac atgctggaaa agctgcagcg cctactgggg ctccgtaccg atccggtcat
     6541 cttcttcacc tcggccgggc tcaccgtgct gttcgtggtc gcggcggtct cgctgacgga
     6601 gcaggtggac cggatcttcg ccacggcctc ggactggctg ctcacccaac tgggctggtt
     6661 ctacatcctg ggtgtgacga ccttcctggt ctatctgctc tggatcgccg tcagccgcta
     6721 cggacacatc cgcctgggcg cccaggacga ccgcccggag tacagcacgc ggacctggtt
     6781 cgcgatgctg ttcgccgccg ggatcggcac gatcctgatg ttctggggcg tggccgagcc
     6841 gatcagccac ttcgccgagc cgccccagca gggcgtcgag ccggagagca ccgaggccgc
     6901 ccggatggcg atggcctact cgctgtacca cttcggcctg cacacctggt cgatcttctg
     6961 cctgccgtcg ctgacgttcg cctacttcgt ctacgtgcgc gggatgccgt tccgggtcag
     7021 ctccgtgttg taccccctcc tgggcgaccg ggtgtacggc acggcgggcc ggatcgtgga
     7081 catcgcggcg gtgatgggca cgctcttcgg cgtcgcggtc tccatcggcc tggggacgat
     7141 gcagatcaac agcgggctgg cgcggctgtt cggtctgccg gagagctcgc tggtgcaggt
     7201 gctgctgatc accgtgatca ccgcgatcgc caccacctcg gtggtctacg gcctggacaa
     7261 gggcatcagg cggttgtcga acatcaacat cgtcatggcc gtcgggctga tgctcttcgt
     7321 gctcgccacc ggggcgacgc tgttcctggc caaggggatc atcgagaacg tcggcgtcta
     7381 tctgtcctcg ctggtgtcgc tggccttctg gaacgacacc ttcgccgaca ccggctggca
     7441 gggcacctgg acggtgttct actgggcgtg gacgatcacc tggtcgccgt tcgtgggcat
     7501 cttcatcgcc cgcatctcca ggggccgcac gatccgcgag ttcgtcctcg gcgtcctcgc
     7561 gctgcccacg gccttcacca tcgtgtggtt cagcgtcttc gggctgtccg ccatcgacat
     7621 cgagatgaac ggtgcgggcg ggctggtgcg gaacgtggtg gaggagggcg acatccccgg
     7681 cgcgctcttc accttcctgg agaactaccc gctcaccacc ttcgtctcgg cgctgtcggt
     7741 actgatcgtg gtcatcttct tcaccacctc ctcggactcg gcctccctgg tggtggacat
     7801 gctcagcgcc gggcagcggg aggagggcgg gccgacgcgg cagcgggtgt tctgggcgat
     7861 caccgagggc gtggtcgcgg ggacgctgat cgcggccacc ggcaagagcg gcctggacgc
     7921 gctcgccgag gtgatcacgg tgctcggtct gccgttcttc gtcctggcgt ttttgatgat
     7981 gtactcgctg gtccgcgcgc tgcggacgga tttccccgaa ctcaccggcc cgcccgccgc
     8041 gctgcggcgg cggatggagc gggagcgcag gacgcgggag tggggcgcgg gcaagcggga
     8101 tggcgaggac ggcaagggcg gcgaggggga gacatccgag ggcgctgggg gagccgaggg
     8161 tgccgagggt gccgagggcg ccgagggaac cgggggagag gcctccgaga gcgggcggta
     8221 ggccctctca gtcgccgccg ccctgccgga ccgggagcgc cgggtgttcg gccacgaccg
     8281 tggccgagcc cggggcgatc tcggtgaaac cggcatcgcg caccactggc aggcccgact
     8341 ccgtcagccg ctcccaccgc tcgcgcgggg cggtgcggac cgcgagcggg aatccggccg
     8401 cgcgccaggc ggcccgcgcc gcgccgtcca gcgccagcca ggcgagctgg gcgccgtgcc
     8461 cggcctgggc catcgccttg ccggcggaca tctccagttc cgggttgagc cacagcacgg
     8521 gcgtgcccgg ctccggcgcg gcgggcggca tggggtcctc cagttcggtg ccggagacct
     8581 ggaggcgggc caggtccctg ggccagccgt ccagcggtac gggcgggaac acccgtacct
     8641 cggccgggtg ctcgccgccg ccggtcaccg tggcgcccgg cagggcgccc gcccgccgcc
     8701 actccgcgcc gcgcgcccgg cgcaccacct tgcggatgcg cccgtcctgc cagtcgcgca
     8761 ccgcccggtg ccagtcgccg ccggggtccg tcgcgcgttc gtccgacagc atcaccagca
     8821 ccgcgcgcgc cgccgtctga agcgcgtcgg tgcgcgcggg cgggccgggg ggagcggacc
     8881 gctcgatccg gacgacgagc ggcagcacgt actgcggggc cgcgtcccgg tcggcggcct
     8941 cgcgggcggc gggggcggtg gagggggcgt cggaggtgct cacggggcca gtgtgccagc
     9001 cgccgccggg gcgtccgcac ccgtttacgt gcgaggtccc gggcgggcgg ggccgggagt
     9061 accctcggcc ccatggagtt cgtatccggc tggaccgcac ccgagacgac ggccgtccgg
     9121 gacgccggtg ccgtccccgc ccccgacggc accgggcgcg aggagcgggc cgtgtgcgtg
     9181 cagtgcggcg agccgaccga ctacccggcg agcacgcgcg gcgcgacgct gtgcccggtg
     9241 tgcgagtggc acgaggccga acgcgtcgcc tgctcctgct gaacggggcc ggaggaagcc
     9301 ggacggagcc ggacggaccc tccggggacg gggctcagtc ccggcccatg gccgaggcga
     9361 tcatctccga gacgtgcacg acgcggtatc cgcgctcctt cagctccggg acgatccggc
     9421 ggacggcctt ctcggtggcc ggcgccttct cgcggtcgca ccgcagcacc accaccgagc
     9481 ccggcctgat gtccttcagc acccgctcgg ccaccttctc cgggtccttc tccccggcgt
     9541 ccggcgccac cacgtcaccg gccaccgccg tcaccttcgc cggggcgatc gtccgccggg
     9601 agcggtcgtc gaagcagccc ccggggaagc ggaagtaggg cgtcaggttc ttcacccccg
     9661 ccgcgcggat cgccttccgc cccttgcgca cgtccgcgag ctgttcgccg ggcgcgagcg
     9721 cggggaggtc gcggcagtcg tcggtgaagg cgcggtggga gtgggagagg ttgccgacct
     9781 cgaacaggtc gtcgcggccg atggagcgcg cctggtgctt gtaggtctgc gcccacaggc
     9841 ccgacatgaa gacggtcgcg gggaccttct cgcggcgcag cgcggcgatc agtccggggt
     9901 tgtcgtggcg ctcgccctcc tcctcggcgc gctcgcggtc ctcggcgggc agggcggcgt
     9961 cgaaggtcag cgccaccacc ttgtcgcccc ggtcaccgct cttctcggcc ttcttccgct
    10021 tctcccggtt ctcgtcgtcc tccgccggct tctcctccgc cggcttctcc tccggcttcc
    10081 cgctctcgtc gtcggtggcc tgcggggccc cggcgcgcgg gccgtgccgg aagacaggca
    10141 cccggccgct gatggaggcg ggcagggtgg cgtaccggtc ggacggcgac acgtcctggt
    10201 cgtcgccgtc cgggcgggag accgaggagc gcggggaggc ggagaagccg tccttctcgg
    10261 agccttcctc cgaggccgcc ggtgaggagg gcgcggccgg ggaggcgccg gcccgggtgg
    10321 tatcgccacc cgggcccgcg cagcccgccg ccgcggcggc gaccagggcg agagcggtca
    10381 ggggacgggc ggtgcggcgc acggagacgg tcatgatcgg aacgtaacga cgatccggga
    10441 cggtcgcgcg ggctgctgct cttgtcggac taccgccacc cccggcccgg gtgacgcccg
    10501 gccgggtggc ggcgcgccgg cttcgccccc gccccgcgca ccggcccatg caccggcccg
    10561 cgcaccgccc cgtacgccgg accgtgggtc aggtcgtgcg ccagtagccc agtgcgtgca
    10621 ccctgtcctt cggcacgccg agctccttgc gggcgtacga ggcgagggcg cgggtggtgg
    10681 tggtgtcgca ggcgatccag acgtagggat ccggctcgtc ctccagcagc ttcggcaggg
    10741 ccgcggtcac ctcggtcacc agatgggcgc cggcgtcccg gcgcgggatc caccgtacct
    10801 cgtgcgggcc ggaccggagc cgcagcggca gttcggcgtc ctgcggatgc gcggtctcca
    10861 gccagacggt cgcgggcgtg ccggggaagg cgtccagcag cgagttgacc gccggcaggg
    10921 acgccgggtc gccgacggcc aggagccggg agggctcggg cgacggcgcg gtgaaccccg
    10981 tgccctggac ggtggcctcg atcgtgtcgc ccggccgcgc gccgcgcgcc cagtcgcagg
    11041 cgatgccctc gtgcagcgcg aactccaggg cgaacgtccc ggccgcggga tcgggatcga
    11101 ccagggtgta ggcgcgctgg tgcggtttgc ccccgctctc gaaccagagg cggacccaca
    11161 tcgtcgggtg gacgccggtc gaggcgagca tcccgccgtc cgtcatccgg atccgcaggt
    11221 agtgctcggt gacctgttcg gttccggtca cggtgaactc gaagtccctg ccc
//
