LOCUS       NZ_FOSG01000003        10405 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces pini strain PL19, whole genome shotgun sequence.
ACCESSION   NZ_FOSG01000003
VERSION     NZ_FOSG01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces pini
  ORGANISM  Streptomyces pini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900114215.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:14:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,762
            CDSs (total)                      :: 5,677
            Genes (coding)                    :: 5,560
            CDSs (with protein)               :: 5,560
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 20 of 117
            Pseudo Genes (incomplete)         :: 111 of 117
            Pseudo Genes (internal stop)      :: 1 of 117
            Pseudo Genes (multiple problems)  :: 15 of 117
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 73656
            Orig. end                         :: 84061
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOSG01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(314..328)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.75"
                     /tool="antismash"
     misc_feature    complement(477..492)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 18.39"
                     /tool="antismash"
     gene            complement(756..1853)
                     /locus_tag="BM237_RS05070"
                     /old_locus_tag="SAMN05192584_10377"
     CDS             complement(756..1853)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238577.1"
                     /locus_tag="BM237_RS05070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10377"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_093848274.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.9e-20, bitscore:
                     67.8, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSERGNFHIRGRVLTGPGPDDVQGEMWVVDGRITYQRPTGPLEAT
                     TVEGWVLPGLVDAHCHVGLDAHGAVDDATSEKQALTDREAGTLLIRDAGSAADTRWIDD
                     REDLPRIIRAGRHIARTRRYIRNYAHEIEPGDLVAYVRREARRGDGWVKLVGDWIDREQ
                     GDLTACWPRGAVEAAIAAAHEEGARVTAHCFAEDSLRDLVEAGIDCVEHATGLTEETVP
                     LFAERGVAIVPTLVNIATFPRLAEGGEEKFPRWADHMRRLHERRYDTVRAAYDAGIPVF
                     VGTDAGGSLPHGLVADEVAELVAAGIPPVDALSAATWAARAWLGRPGLEEGAEADLVVY
                     EEDPRADVRVLAAPRAVLLRGRPVR"
     gene            1949..2509
                     /locus_tag="BM237_RS05075"
                     /old_locus_tag="SAMN05192584_10378"
     CDS             1949..2509
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019060605.1"
                     /locus_tag="BM237_RS05075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10378"
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_093848276.1"
                     /transl_table=11
                     /translation="MDAPAEEFHAFFDRHYADLSRFAHLLLGDSEGADDLAADALVAVW
                     RRWDSVRVGGHPLAYARSVVAGMARTRLRSLLREHRRVALFWSHRPDRIDDPDVPAVLD
                     VRDALARLPLRKRTCVVLRHAFDLSEKETARTLGVSVGTVKSQTSRGIAELERMLAPAR
                     TGSGRAHGTAGALRAGSAGRSGW"
     misc_feature    2744..2761
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.31"
                     /tool="antismash"
     misc_feature    complement(2890..2906)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.6"
                     /tool="antismash"
     gene            2978..3514
                     /gene="ectA"
                     /locus_tag="BM237_RS05080"
                     /old_locus_tag="SAMN05192584_10379"
     CDS             2978..3514
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358302.1"
                     /locus_tag="BM237_RS05080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10379"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_093848278.1"
                     /transl_table=11
                     /translation="MPEGLKLDAPRVEDGAAIWRIARDSRVLDLNSSYSYLLWCRDFAA
                     TSVVARDGAGEPVGFITGYVRPQHPRTLVVWQVAVDHGQRGRGLAAAMLDGLTIRTAEE
                     LGVDRIETTITPDNTASNRLFTSYAERHGAPVRREVLFEGALFPEEGHEPEVLYRIGPV
                     PTLAAAAPPPAAADG"
     gene            3597..4865
                     /gene="ectB"
                     /locus_tag="BM237_RS05085"
                     /old_locus_tag="SAMN05192584_10380"
     CDS             3597..4865
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 1e-76.
                     Score: 250.1. Matches aSDomain:
                     nrpspksdomains_BM237_RS05085_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="BM237_RS05085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10380"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_093848280.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2e-82, bitscore:
                     271.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMTQPDLSIFQTLESEVRSYCRGWPTVFDRARGSYMYDEDGHAF
                     LDFFAGAGSLNYGHNNPVLKRALLDYLERDGVTHGLDMSTTAKRAFLETFREVVLQPRG
                     LDHKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDRYLDGKVEDFLWFERLLEDSGSGLNRPAAVIVETVQGEGGINVARA
                     EWLRALADLCKRQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIITVSKSISGYGMPMA
                     LTLFRPELDVWEPGEHNGTFRGNNPAFVTAAATLNTYWADGQMEKQTLARGEQVEAALR
                     AIAAEHPEEVADVRGRGLVWGMEFHDKDRAGSVASRAFELGLLIETSGPESEVVKLLPA
                     LTISPEELDEGLRILARAVRETA"
     aSDomain        3681..4691
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM237_RS05085_Aminotran_3.1"
                     /evalue="1.00E-76"
                     /label="BM237_RS05085_Aminotran_3.1"
                     /locus_tag="BM237_RS05085"
                     /protein_end="365"
                     /protein_start="28"
                     /score="250.1"
                     /tool="antismash"
                     /translation="FDRARGSYMYDEDGHAFLDFFAGAGSLNYGHNNPVLKRALLDYLE
                     RDGVTHGLDMSTTAKRAFLETFREVVLQPRGLDHKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDRYLDGKVEDFLWFER
                     LLEDSGSGLNRPAAVIVETVQGEGGINVARAEWLRALADLCKRQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIITVSKSISGYGMPMALTLFRPELDVWEPGEHNGTFRGNNPAFV
                     TAAATLNTYWADGQMEKQTLARGEQVEAALRAIAAEHPEEVADVRGRGLVWGMEFH"
     gene            5001..5405
                     /locus_tag="BM237_RS05090"
                     /old_locus_tag="SAMN05192584_10381"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141824.1"
                     /locus_tag="BM237_RS05090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10381"
                     /product="ectoine synthase"
                     /protein_id="WP_093655460.1"
                     /sec_met_domain="ectoine_synt (E-value: 7.7e-55, bitscore:
                     178.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLQEIEDTDRHVRSASGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHVEAVLCVEGEAELTNDETGEKHWITPGTMYLLDGHEKHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPDES"
     gene            5543..6442
                     /gene="thpD"
                     /locus_tag="BM237_RS05095"
                     /old_locus_tag="SAMN05192584_10382"
     CDS             5543..6442
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671935.1"
                     /locus_tag="BM237_RS05095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10382"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_093848282.1"
                     /sec_met_domain="PhyH (E-value: 2.2e-39, bitscore: 130.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGASEVITPRQDPTVWSAPGTPGPIETADLE
                     SYDRDGFLAIDQLITPDEVEIYNRELNRLVSDPLIRADERSIVEPKSQEIRSVFEVHKI
                     SEVFANLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYETNGALMIMPGSHRTFLGCEGATPKDNYKKSLQMQDAGTPSDEA
                     LARLAGEHGIRLFTGKPGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENTAVEPFA
                     APVPRPGFLGARDFTPVR"
     gene            complement(6649..8655)
                     /locus_tag="BM237_RS05100"
                     /old_locus_tag="SAMN05192584_10383"
     CDS             complement(6649..8655)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174265.1"
                     /locus_tag="BM237_RS05100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10383"
                     /product="beta-galactosidase"
                     /protein_id="WP_093848284.1"
                     /transl_table=11
                     /translation="MVRLAYGGDYNPEQWPEEVWHEDVALMREAGVTTVSVGIFSWASI
                     EPAEGVFDFGRLDRIVGLLHEAGIGVDLATPTVVPPAWFYRAHPEALPVSREGVRWEFG
                     SRGAICHNSPAYQEASARITRALGERYGRHPAVVMWHVHNEYGVPVLECYCEISAAAFR
                     TWLRERHTTLEKLNAAWGTAFWGQTYGDWEEIAPPRATPTVCNPAQRLDYARFASDSAL
                     ANFRRERDLLHELSPGVPVTTNFMVSPTQCDTIDYWAWGREVDIVTNDHYLVAEDERNH
                     VNLSMAADLTRSVAGGEPWLLLEHSTSGVNWQPRGIAKRPGEMARNSLAHIARGSEGAM
                     FFQWRASRSGAEKFHSAMVPHAGTDSRIWREVVSLGADVAALEEIRGTRTVADVAVVWD
                     WQSWWAQRLEWRPSQDLDAREHAEAWYAALFDRGLTADFVPPHTGAAALAAYPLVVVPA
                     LYLATAQAGENLRAYVAGGGTLVVSYFSGIVDEHDAVHPGPHPGALREVLGLTVEEFQP
                     LRAGRSVPVLGPGVPSLEADVWAETVVTRGAETVWSYGCGPAAGGPAVTRNRLGEGTAW
                     YVSTRLGQGGLDAVLAAACADAGIAPRDEIPHDVEVVRRSGEPGTYLFVLNHTDADAEV
                     PLEAPGTELLGGARVAGRLAVPAGGVRVVRLDG"
     gene            8996..10027
                     /locus_tag="BM237_RS05105"
                     /old_locus_tag="SAMN05192584_10384"
     CDS             8996..10027
                     /GO_function="GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078: LacI family
                     transcription regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018553304.1"
                     /locus_tag="BM237_RS05105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10384"
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_093848286.1"
                     /transl_table=11
                     /translation="MSGSERRRPPTIHDVAREAGVSRGTVSRFLNGGHNVSPAAADAVR
                     AAIRKTGYVVNRHARSLITGRSGSVAFLLTEPQEKLFEDPNFNVLLRGCTRALAAHDIP
                     LLLMTAGTPAERRRVVRYVTAGHVDGVLLVSSHTGDPVAEQLLKAGVPVVAGGRPVDRG
                     GRTVRIAHVTADDRDGARGIVRHLVSLGRRRIATVAGPPDTPGGVDRLAGWREALAEAG
                     LPAGDELVAHGDYSRAGGRRAAAELLERVPGMDALFVASDLMARGALTALERAGRRVPD
                     DVAVGGFDDSPAAVAGLPELTTVRQPWDRISSEMVRVLLGLIGGEEPSAVILPTELVRR
                     QSA"
ORIGIN
        1 tcgccgagca ccttgacctt gccgacgaac agggaccgcg cggtcggctc ggcaccggcc
       61 tcgcacaccg cctcggaggt gaccttctcg acgtggacga gcggcttcgt accagggatc
      121 cccttcgccg ttccgggcag gtacaccttg gcctctgcca ggttggagta cccctcggcc
      181 ctcccctcct ccaccgtcgc cctcgaggtg gcggccttcg cgtccagcat ggtgaacggc
      241 tttccgccgt tcgcgccgtc cacccgggct tccagcaggg tctcgtcggc gctcctggga
      301 gccttgaccg agttgagctc gatgttcagc ggtacggcgg cgccgccctt gagcagggag
      361 acctccaggt tcgcccggag tacggccgcg ctcgccgagc cctcgtcgcc ggcggcgttc
      421 gcgggcgcgg cggtgacccc caccagagcg gcggcgagca ggccgtagac agacttgttc
      481 acaggtgaaa cccccacgga ttgcatgacg tcaccggcgg ccaatagggg acatcgcccg
      541 caccggtgac tgagaccacg ccattgttga cccacgtaga gcgaaagagc gaatacatag
      601 cgttagttca ctcgaaagag tcacaccagg ggtagttttc gattcgtgga tcctgggtag
      661 cgcctatttt cttcgcaacg cgggcagacg cgggcccacg acgccccctc cgcctgcggc
      721 ccctgccggc gcaccgcacg ccctcacgca ccccgtcacc tcacgggacg ccccctgagc
      781 agcaccgccc gcggcgcggc cagcacccgt acgtcggccc gcgggtcctc ctcgtacacc
      841 accagatccg cctcggcgcc ctcctccagc cccgggcgcc ccagccaggc tcgggccgcc
      901 caggtggccg ccgacagggc gtccaccggc gggatgcccg ccgccaccag ctccgccacc
      961 tcgtcggcga ccagcccgtg gggcagcgag ccgcccgcgt ccgtgccgac gaagaccggg
     1021 atgcccgcgt cgtacgccgc gcgcacggtg tcgtagcggc gctcgtggag ccgccgcatg
     1081 tgatcggccc agcgcgggaa cttctcctcg ccgccctcgg cgagccgggg gaaggtcgcg
     1141 atgttgacca gggtggggac gatggcgaca cctcgctcgg cgaagagcgg gactgtctcc
     1201 tccgtcaggc cggtggcgtg ctcgacgcag tcgatcccgg cctccaccag atcgcgcagc
     1261 gagtcctcgg cgaagcagtg cgcggtgacc cgcgcgccct cctcgtgggc ggcggcgatg
     1321 gcggcctcca ccgcgccgcg cggccagcag gcggtcagat cgccctgctc gcggtctatc
     1381 cagtcgccga cgagcttgac ccagccgtcg ccgcgccgcg cctcgcgccg cacgtacgcc
     1441 accagatcgc cgggctcgat ctcgtgggcg tagttgcgga tgtagcgccg ggttcgggcg
     1501 atgtggcggc cggcccggat gatgcgcggc aggtcctcgc ggtcgtcgat ccaccgggtg
     1561 tcggccgccg atcccgcgtc ccggatgagc agcgttccgg cctcgcggtc ggtgagtgcc
     1621 tgcttctcgc tggtggcgtc gtcgacggcg ccgtgggcgt cgagtccgac gtggcagtgt
     1681 gcgtccacca ggccgggcag cacccagccc tcgaccgtgg tcgcctccag cggcccggtg
     1741 ggccgctggt aggtgatccg cccgtccacg acccacatct cgccctggac gtcatcgggg
     1801 ccgggcccgg tcaggacccg tccccggatg tggaaattcc cgcgctcgct catatgccgc
     1861 actgtacgag tcccctgtta cgctcacgca gccgcagggg gtcgtggcac accacttcac
     1921 aggatccgtc gagccctgag gaggccacgt ggacgccccc gccgaagagt tccacgcctt
     1981 cttcgaccgg cactacgccg atctgtcccg cttcgcccat ctgctgctgg gagactccga
     2041 gggagccgac gacctcgccg ccgacgcact cgtcgcggtg tggcgccggt gggacagcgt
     2101 gcgcgtcggc ggacacccgc tggcgtacgc gcgcagcgtg gtcgccggta tggcacgcac
     2161 ccggctgcgc agcctgctgc gcgagcaccg ccgggtcgcc ctgttctggt cgcaccggcc
     2221 cgaccgcatc gacgacccgg acgtgcccgc ggtactggac gtgcgtgacg cgctcgcccg
     2281 gctgccgctg cgcaagcgga cgtgtgtcgt tctgcggcac gccttcgacc tgtcggagaa
     2341 ggagaccgcc cgcaccctgg gcgtctcggt cggcaccgtc aagagccaga cctcgcgcgg
     2401 tatcgccgaa ctggaacgga tgctggcgcc ggcccggacg ggttccggcc gcgcgcacgg
     2461 gaccgcgggg gcgctgcgcg ccggatccgc gggacggagc ggttggtgac cgtggtggcc
     2521 gaccggtccg cccggccgga ccggtggcgg cggaggggga acttccgccg agttccccga
     2581 agaacctcac gatcaggttt cgctgaggta tcttccatga atcaaagatg aatttacggg
     2641 cacctgacga gaatagaatc ccatatcctc atgcccgctc gcgcgatcct taaacactaa
     2701 gatttgatat cggcgaacct cgtatttcgg acaggttccg tgctcgttat ggatctgtga
     2761 cggactcaac agtgattcct ctttgcccgg aaaatacggg gcataccagg cataacgcag
     2821 cgcccttcgt gcgcgtgcgc acccacgcgc gataacatat gacgcgctcc tgcgtaaccc
     2881 cttccctcga tgtgatttga gttcgctctg ggtaaattgg atttgcatga ccgccgcaca
     2941 agcagaccat gcaggtgccc acagaaagac cgtcgaaatg ccggagggac tgaagctcga
     3001 cgcgccccgc gtggaggacg gcgcagcgat ctggcggatc gcgcgcgact cccgggtcct
     3061 cgacctcaac tcctcctaca gctacctcct gtggtgccgc gacttcgccg ccacctcggt
     3121 ggtggcgcgc gacggcgccg gtgagccggt cggtttcata accggctacg tccggccgca
     3181 gcacccgcgg accctggtcg tctggcaggt cgccgtcgac cacgggcagc gcggacgcgg
     3241 cctcgccgcg gcgatgctcg acgggctgac catccgtacc gccgaggaac tgggcgtcga
     3301 ccggatcgag accacgatca cccctgacaa caccgcctcc aaccgactgt tcacctccta
     3361 cgccgagcgc cacggcgcgc cggtgcggcg cgaggtcctc ttcgagggcg cgctgttccc
     3421 cgaggaggga cacgagcccg aggtgctgta ccggatcggg cccgtcccca ccctggccgc
     3481 cgccgcgccc ccaccggccg ccgccgacgg ctgaccgccg cgcggcgcgc cgcaccgcgc
     3541 cccggcaccc gaaagacctc ccactcctct cgcaatccat ctcccaggag atcgctgtga
     3601 ccatgaccca gcccgacctg agcatcttcc agaccctcga atcggaggtg cgcagctact
     3661 gccgcggctg gcccaccgtc ttcgaccggg cgcgcggcag ctacatgtac gacgaggacg
     3721 gccacgcgtt cctcgacttc ttcgccggcg ccggatcact gaactacggc cacaacaacc
     3781 cggtgctcaa acgcgccctg ctggactacc tcgagcgcga cggcgtcacc cacggcctgg
     3841 acatgtccac cacggccaag cgcgccttcc tggagacctt ccgggaggtg gtcctccagc
     3901 cgcgcggcct ggaccacaag gtgatgttcc ccggcccgac cggcaccaac gccgtcgagg
     3961 cggccctgaa gctggcccgc aaggtcaagg gccgggagtc gatcgtctcc ttcaccaacg
     4021 ccttccacgg catgtcgctg ggctccctgg ccgtgaccgg caacgccttc aagcgcgccg
     4081 gcgccggcgt ccccctggtc cacggcaccc cgatgccctt cgaccgctac ctggacggca
     4141 aggtcgagga cttcctgtgg ttcgagcggc tgctggagga ctccggctcg ggcctgaacc
     4201 ggccggccgc cgtgatcgtc gagacggtcc agggcgaggg cggcatcaac gtcgcccggg
     4261 ccgagtggct gcgcgccctg gccgacctgt gcaagcggca ggacatgctg ctgatcgtcg
     4321 acgacatcca gatgggctgc ggccgcaccg gcgccttctt ctccttcgag gaggcgggca
     4381 tcacccccga catcatcacc gtctccaagt ccatcagcgg ctacgggatg ccgatggcgc
     4441 tgaccctgtt ccggcccgag ctggacgtgt gggagcccgg cgagcacaac ggcaccttcc
     4501 gcggcaacaa ccccgccttc gtcaccgccg cggccaccct gaatacctac tgggcggacg
     4561 ggcagatgga gaagcagacg ctcgcccgcg gcgagcaggt cgaggccgca ctgcgcgcca
     4621 tcgccgccga gcaccccgag gaggtggccg acgtccgggg ccgcggcctg gtgtggggca
     4681 tggagttcca cgacaaggac cgtgccggct ccgtcgcctc ccgcgccttc gagctcggac
     4741 tgctcatcga gacctccggc cccgagagcg aggtcgtcaa gctgctcccg gcactgacga
     4801 tctcccccga ggagctggac gagggcctgc gcatcctggc ccgcgcggtc cgcgagaccg
     4861 cctgacaccc gcggcaccgc ccgtccccgg cgcccgttcc ggcgccgggg gcgtccggtc
     4921 gccgcacgca ccacccgaat ggcccatccc gcaggctttt cccgcggatg ggcctctgac
     4981 cggaaaggca ccacttcacc gtgatcgtcc gatcgctaca ggaaatcgag gacaccgacc
     5041 gccacgtcag gtccgcctcc ggcacctggg agagcaagag gatcgtgctc gcgaaggagc
     5101 gtgtgggctt ctcgctgcac gagaccgtgc tctacgcggg cacggagacc tcgatgtggt
     5161 acgccaacca cgtggaggcc gtcctctgtg tggagggcga ggccgagctc accaacgacg
     5221 agaccggcga gaagcactgg atcacccccg gcaccatgta cttgctggac gggcacgaga
     5281 agcacacact tcgtcccaag acggacttcc gttgcgtctg cgtcttcaac ccgccggtca
     5341 ccggccgtga ggaccacgac gagaacggcg tctaccccct gctcaccgaa ccggacgaga
     5401 gctgacgcac cgcaccggcc gagccagacg ggccggtgcg gacggcagcc gagaccacac
     5461 gggaggccct ggcgagagaa cccgcagcga agcggccaca cgcgggtaca gggccgcacg
     5521 atgaggagag gaaggcagaa ccatgaccac cgcacccgaa cgcaccgcag acctctaccc
     5581 gacccggggc gcctccgagg tgatcacccc ccggcaggac cccacggtgt ggtccgcccc
     5641 cggtaccccg gggccgatcg agaccgcgga cctggagtcc tacgaccggg acggcttcct
     5701 ggcgatcgac cagctcatca cccccgatga ggtggagatc tacaaccggg agctgaaccg
     5761 gctggtctcg gacccgctga tccgtgcgga cgagcgttcg atcgtggagc cgaagtccca
     5821 ggagatccgc tccgtcttcg aggtccacaa gatcagcgag gtcttcgcca acctggtgag
     5881 ggacccgcgg gtggtgggcc gcgcccgtca gatcctgggc tcggacgtgt acgtccacca
     5941 gagccggatc aacgtcaagc cgggcttcgg ggcctcgggg ttctactggc actccgactt
     6001 cgagacctgg cacgccgagg acggtctgcc gaacatgcgg acggtctccg tctcgatcgc
     6061 cctgaccgag aactacgaga ccaacggcgc tctgatgatc atgcccggtt cgcaccggac
     6121 gttcctcggc tgcgagggcg ccacgccgaa ggacaactac aagaagtccc tgcagatgca
     6181 ggacgcgggc acgccgtccg acgaggcgct ggcccggctc gccggtgagc acggcatccg
     6241 cctgttcacg ggcaagcccg gttcggcgac ctggttcgac tgcaactgca tgcacggttc
     6301 cggggacaac atcaccccgt acccgcgcag caacgtcttc atcgtcttca acagcgtgga
     6361 gaacaccgcg gtggagccgt tcgcggcgcc cgtcccgcgc ccggggttcc tcggcgcacg
     6421 ggacttcacc cccgtgaggt gaccatctcc cgcgaggtgg ccgctcacat gaggtgagga
     6481 gtgcgcggcg tcccgtttcc ccgccgggcc gttccgtacg gcccggcggg gaaacgggac
     6541 gcgaaggcgg aaggggggcg ggaccgccac ggcggttccg ccccccttcc gccttccccg
     6601 ctcgccgacc cgcccgcggg tcggcgagcg gggcggggct cctgtggctc agccgtcgag
     6661 acggaccacg cggaccccgc ccgcggggac cgcgagccgg ccggccacgc gggcgccgcc
     6721 gagcagttcg gtgccgggcg cctccagcgg cacctcggcg tcggcgtcgg tgtggttgag
     6781 gacgaagagg taggtgcccg gctccccgga gcggcgcacc acctccacgt cgtgggggat
     6841 ctcgtcgcgc ggggcgatgc ccgcgtccgc gcaggcggcg gccagcacgg cgtcgagccc
     6901 gccctgcccc agccgcgtcg agacgtacca cgcggtgccc tcgcccagcc ggttacgggt
     6961 gacggcgggg ccgcccgcgg ccggtccgca gccgtaggac cacaccgtct cggcgccccg
     7021 cgtcacgacg gtctcggccc atacgtcggc ctccagcgag gggaccccgg ggccgaggac
     7081 gggcacgctc cggcccgccc gcagcggctg gaactcctcg accgtcagcc ccagcacctc
     7141 gcgcagcgcg cccgggtgcg ggccggggtg gacggcgtcg tgctcgtcga cgatgccgga
     7201 gaagtacgac accaccaggg tgccgccgcc ggcgacgtag gcgcgcaggt tctccccggc
     7261 ctgtgccgtg gccaggtaga gggcggggac caccaccagc ggataggcgg ccagagcggc
     7321 cgccccggtg tgcggcggga cgaagtcggc cgtcagaccg cggtcgaaga gagccgcgta
     7381 ccaggcctcg gcgtgctcgc gggcgtcgag gtcctggctg gggcgccact ccagcctctg
     7441 cgcccaccac gactgccagt cccacaccac cgccacgtcc gcgacggtgc gggtaccgcg
     7501 gatctcctcc agcgccgcga cgtccgcgcc gagcgagacc acctcgcgcc agatccggct
     7561 gtcggtgccg gcgtgcggga ccatcgccga gtggaacttc tccgcaccgg agcgggaggc
     7621 gcgccactgg aagaacatcg cgccctccga gccccgggcg atgtgggcca gggagttgcg
     7681 ggccatctcg ccggggcgct tggcgatgcc gcgcggctgc cagttgacgc cgctggtgga
     7741 gtgctccagc agcagccacg gctcgccgcc ggcgaccgag cgggtcaggt cggcggccat
     7801 cgacaggttg acgtggttgc gttcgtcctc ggccaccagg tagtggtcgt tggtgacgat
     7861 gtccacctcg cggccccagg cccagtagtc gatggtgtcg cactgggtgg gcgagaccat
     7921 gaagttggtg gtgacgggga cgcccgggga cagctcgtgc agcaggtcgc gctcgcggcg
     7981 gaagttggcc agggcgctgt cgctcgcgaa gcgggcgtag tccaggcgct gggcggggtt
     8041 gcagacggtg ggggtcgccc ggggcggggc gatctcctcc cagtctccgt aggtctgccc
     8101 ccagaaggcg gtcccccagg cggcgttgag cttctccagg gtggtgtggc gctcccgcag
     8161 ccaggtgcgg aaggccgcgg cggagatctc gcagtagcac tccaggacgg ggacgccgta
     8221 ctcgttgtgg acgtgccaca tcacgaccgc gggatgccgt ccgtagcgct cgcccagcgc
     8281 gcgggtgatc cgggcggagg cctcctggta ggcgggcgag ttgtggcaga tcgcgccgcg
     8341 tgagccgaac tcccaccgca cgccctcccg gctgacgggc agcgcctccg ggtgggcccg
     8401 gtagaaccag gcgggcggca ccacggtggg ggtggccagg tcgacgccga ttccggcctc
     8461 gtgcagcaga ccgacgatcc ggtcgagccg gccgaagtcg aagacgccct cggcgggctc
     8521 gatcgacgcc caggagaaga tcccgacgct gaccgtggtc acaccggcct cacgcatcag
     8581 cgcgacgtcc tcgtgccaga cctcctcggg ccactgctcg gggttgtagt cgccgccgta
     8641 ggcgaggcgc accatgcgac ctcctgggtg gaagctccgg cccgagtggg ccggaccggg
     8701 acgggaatgg gaacgttcac agacgcttgc cgactgtgtc acccagacaa caccaagctt
     8761 cgaaccattg acaagagtca gccgactctc cctcctgtgc acgttcacag catcttgtcg
     8821 gggagggaac acgaaccgcc gcgccccccg tgcacccggg cgtgaacgcc cggggttccg
     8881 cgccggatca cgctgaaggg cggccggcga gtgcgcgggg atcaccgaca attgcacgtg
     8941 agacggggac acggggagat ggggagatgg ggacggacgc gaggatgggt accgcatgag
     9001 cggcagtgag cggcggcgcc cgccgacgat ccacgacgtg gcgcgggagg ccggggtctc
     9061 ccgaggcacg gtctcgcggt tcctcaacgg cgggcacaac gtcagcccgg ccgcggcgga
     9121 cgcggtcagg gccgcgatac gcaaaacggg ctacgtcgtc aaccggcacg cccgcagcct
     9181 gatcaccggc cgttccggtt cggtggcctt cctgctcacc gaaccgcagg agaagctgtt
     9241 cgaggacccc aacttcaacg tgctgctgcg cggctgcacc cgggcgctgg ccgctcacga
     9301 catcccgctg ctgctgatga ccgccggtac gcccgccgag cggcgccgtg tcgtgcgcta
     9361 cgtcaccgcg ggccatgtcg acggcgtcct gctggtgtcc agccacaccg gcgatccggt
     9421 ggccgagcag ctgttgaagg ccggcgtacc ggtggtcgcc ggcggcagac cggtcgaccg
     9481 cggcgggcgt accgtcagga tcgcccatgt gacggccgac gaccgcgacg gcgcccgcgg
     9541 catcgtgcgg cacctggtct cgctcgggcg ccgccgcatc gccacggtcg ccgggccgcc
     9601 ggacaccccc ggcggggtgg accggctggc gggctggcgc gaggcgctgg ccgaggccgg
     9661 gctcccggcc ggcgacgagc tggtcgccca cggcgactac agccgcgccg gcggccggcg
     9721 ggccgccgcc gaactgctcg agcgggtacc ggggatggac gcgctgttcg tcgcctccga
     9781 cctgatggcg cgcggtgcgc tgaccgcgct ggagcgggcc gggcggcggg tgcccgacga
     9841 tgtcgccgtc ggcggcttcg acgactcgcc ggccgccgtc gccggactcc cggagctgac
     9901 caccgtccgc cagccgtggg accggatcag ctcggagatg gtgcgggtgc tgctgggtct
     9961 gatcggcggc gaggagccgt cggcggtgat cctgccgacc gaactggtgc gcaggcagtc
    10021 ggcgtgaccg caaggccccg gggcacgccg gccccggggc cttcggccgc tcggggtcac
    10081 acccggggca gcgccgccag cagccgctcc acgtcctcgg ccgtgttgta caggtggaag
    10141 gccgcgcgca ggttcccctc ccggacggag acctccaccc ccgcctccct cagcgcgtcg
    10201 accgccccgc ccaggtgggg caccgcgacg atggccgagt cgccgggcac cgggcggtgg
    10261 cccagcccgg cggcgccggc gcggaagcgg gcggccaggg cccggtcgtg ggcgccgatc
    10321 gcctccggcc cggcctcctc gaccaggccc agggagcgct cggcgcccag gtagggcagg
    10381 aaggccgggg agacgtcgaa gcggc
//
