LOCUS       NZ_FOSG01000002        13012 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces pini strain PL19, whole genome shotgun sequence.
ACCESSION   NZ_FOSG01000002
VERSION     NZ_FOSG01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces pini
  ORGANISM  Streptomyces pini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900114215.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 02:14:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,762
            CDSs (total)                      :: 5,677
            Genes (coding)                    :: 5,560
            CDSs (with protein)               :: 5,560
            Genes (RNA)                       :: 85
            rRNAs                             :: 6, 4, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 1 (5S, 16S)
            partial rRNAs                     :: 3, 6 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 20 of 117
            Pseudo Genes (incomplete)         :: 111 of 117
            Pseudo Genes (internal stop)      :: 1 of 117
            Pseudo Genes (multiple problems)  :: 15 of 117
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:04:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 76413
            Orig. end                         :: 89425
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOSG01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13012
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="1"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..13012
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13012
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8012](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..8012)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    237..252
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.96"
                     /tool="antismash"
     gene            complement(713..1426)
                     /locus_tag="BM237_RS03075"
                     /old_locus_tag="SAMN05192584_10263"
     CDS             complement(713..1426)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128212.1"
                     /locus_tag="BM237_RS03075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10263"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_093847543.1"
                     /transl_table=11
                     /translation="MSELKPRKLVSVQEHLRDQVANALRAALIAGELRPGVIYSAPTLA
                     AEYGVSATPVREAMLDLAHEGLVESVRNKGFRVTELSERDLDEFTEIRALIEIPTVGKV
                     ARSATREQLEALRPTAEEIVAAARAGDLIGYLEADRRFHLGLLALAGNSRLVETVGDLR
                     KRSRLYGLTELAEAGALVASAREHAELLDFMLAGDAAAAEEHMRHHLAHVRSLWAARKQ
                     ETPEQKSPARKLNAR"
     misc_feature    1455..1471
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.35"
                     /tool="antismash"
     misc_feature    1459..1469
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            complement(1550..2971)
                     /locus_tag="BM237_RS03080"
                     /old_locus_tag="SAMN05192584_10264"
     CDS             complement(1550..2971)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608086.1"
                     /locus_tag="BM237_RS03080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10264"
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="WP_093847545.1"
                     /sec_met_domain="Aldedh (E-value: 9.1e-131, bitscore:
                     431.1, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTPPAPAADSPVISRNPADPSDILIELYGCGPETATRAVRRAAR
                     AQSEWLAAGAAARSAALRRAGDAVEIASEELAGLLVREVGKPLAEARGELARTAAIWRY
                     YAQLPYDATGAVHEPASGPGLLLTRRRPHGVAGLITPWNFPLAIPSWKAAPALAAGNAV
                     VLKPAPEATACALRLAEILKEVLPADVLQVVPGGAEAGAALIGLADVVSFTGSTAVGAV
                     VARAAAARGIPAQAEMGGHNAAVVLPDANIGKAAADIAAAIAGYAGQKCTATRRVIAVG
                     SALPKLREALAEALRELPARDPHAAAAVCGPVISKAARDRVVRARDSALSTGARALAGG
                     GRRGSGAAAGWYVDPVLLENVPAAHELHRREIFGPIAVLSGAADFDEAVQAANSGHYGL
                     VASLHTGDLAKALSAVDRLTAGMLRVNAPTTGVDFHLPFGGEKDSSYGMREQGHAAMDF
                     YTSSRTVSLLPAASA"
     gene            complement(2968..3969)
                     /locus_tag="BM237_RS03085"
                     /old_locus_tag="SAMN05192584_10265"
     CDS             complement(2968..3969)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358096.1"
                     /locus_tag="BM237_RS03085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10265"
                     /product="proline racemase family protein"
                     /protein_id="WP_093847547.1"
                     /transl_table=11
                     /translation="MRSRRVFHAVDSHTEGMPTRVITGGIAPLPGATMAERRTYFMRNL
                     DHIRTLLMYEPRGHAAMSGAILQPPTRPDADWGVIYIEVSGCLPMCGHGTIGVATVLVE
                     TGMVPVTEPVTTVRLDTPAGLVTAEVHVHDGAARAVTLRNVPSFAVALGEKVEVPGFGR
                     ITYDLAYGGNFYAILPVEELGLPFDRSRKEKLLRAGLEVMAAINTAGEPVHPEDPGITG
                     CHHVQLLAPGSDARRSRNAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLHRDFVNES
                     FLGTSFTGRLVEETEVAGRAAVVPTFTGSAWITGTAQFFLDPTDPFPEGFLL"
     misc_feature    3964..3979
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.52"
                     /tool="antismash"
     gene            complement(4013..4876)
                     /locus_tag="BM237_RS03090"
                     /old_locus_tag="SAMN05192584_10266"
     CDS             complement(4013..4876)
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018839502.1"
                     /locus_tag="BM237_RS03090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10266"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_093848071.1"
                     /transl_table=11
                     /translation="MVATALPFREDLSVDYDAYAEHVRFLLDAGCDGVVPNGSLGEYQT
                     LTDDERARVIRTAVEAAGDGSRVMAGAAAYGSTEAARWTEQAAEAGAGSVLLLPPNAYR
                     ANDAEVRAHYAEVAGVGLPVVAYNNPYDTRVHLTPQLLADLHAQQHIVAVKEFSGDVRR
                     AYEIAECAPGLDLLIGADDVLLELALAGAVGWIAGYPNALPHSCVALYRAAIAQDLETA
                     LPLYRALHPLLRWDTKHVFVQAIKLSMDIAGRHGGPCRPPRSPLDTEAEAAVRSVTEKV
                     LAEGLN"
     gene            complement(5001..6497)
                     /locus_tag="BM237_RS03095"
                     /old_locus_tag="SAMN05192584_10267"
     CDS             complement(5001..6497)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1175: pyridine nucleotide-disulfide oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139732.1"
                     /locus_tag="BM237_RS03095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10267"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_093847549.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 4.9e-27, bitscore:
                     89.4, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 2.2e-09, bitscore:
                     32.2, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTSPAEPSGAPYDLAVVGAGPAGLAAAVTAADAGLTVALVDAEP
                     RPGGQYYRHPAPGLGARRPEALHHGWPAFTTLETRLRQHKQVGRVRHLPGRQVWTIQQS
                     CDHAGRWTLLTTATVPPHDRAAPGDQNTGEAPGESVPAHHLLLATGTYERQLPFPGWTL
                     PGVVGAGGAQAMIKSGLVLPGRRVIVAGSGPLLLAAAASLSAAGARVPYVLEASSYLPY
                     ARRPGPLLRNLGKLAEGARHAAALARRRVGLRTRTAVVQAHGTDRVTGVTVARLDGDWR
                     PLPGTEQHLSCDALAVGHGLVPQLELAVELGCATRTAPDGTPALVVDSFLRTSVEGVWA
                     AGETNGVGGAQLALCEGELAAHAVAAAAHGETTAARTAAALRTRATRLRAFADLMARVH
                     RPGPAWPQWLRGDTEICRCEEIDASSVREAVDTFGAGDTRTAKLLTRAGMGWCQGRMCG
                     PAVSCLVSGDGSAPVSADRRPLSRPVPLVQLARHRPQGRP"
     gene            complement(6478..6792)
                     /locus_tag="BM237_RS03100"
                     /old_locus_tag="SAMN05192584_10268"
     CDS             complement(6478..6792)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017237883.1"
                     /locus_tag="BM237_RS03100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10268"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_093847551.1"
                     /sec_met_domain="Fer2_4 (E-value: 3.9e-27, bitscore: 88.8,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRTPAGLVRAEPHSAISIDYDGRKLPALPGQSIAAALWAAGIVS
                     WRTTRRGGRPRGAFCGMGVCFDCLITVNGRPNQRACLVPAHDGDTVTPQEGDGRADLAC
                     "
     misc_feature    complement(6786..6796)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.79"
                     /tool="antismash"
     gene            complement(6846..8012)
                     /locus_tag="BM237_RS03105"
                     /old_locus_tag="SAMN05192584_10269"
     CDS             complement(6846..8012)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038321.1"
                     /locus_tag="BM237_RS03105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10269"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_093848073.1"
                     /sec_met_domain="DAO (E-value: 1.4e-69, bitscore: 229.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLMRQTVDAVVIGAGVVGAACAYYATRAGLAVAVVDRGSPAGGTT
                     GAGEGNLLVSDKPPGPELDLAQLSAGLWRDLAAQLPEGIEYEAKGGVVVAADDTEWSAL
                     RRMAAGQAAAGVVVREVPADRLPDLEPHLAPGLAGAVHYPQDAQVQPALAASRLLRAAQ
                     NSGAALHLREAVTEVLVTPAGRIDGVRTPHRELKAPVVVNATGTWSAQIAALAGTTLPV
                     MPRKGFVLVTEPLPRVVRHKVYAAAYVADVASDSASLQTSPVVEGTPAGPVLIGSSRER
                     TGFDRTLSTEALRRMADSAVTLFPILAQVSVLRAYHGFRPYLPDHLPAIGADPRVPGLH
                     HACGHEGAGIGLAPATGLLLAQALTGEKPSLDLAPFRPDRFESEVQHA"
     misc_feature    8068..8079
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     misc_feature    8254..8264
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 17.67"
                     /tool="antismash"
     gene            complement(8373..10904)
                     /locus_tag="BM237_RS03110"
                     /old_locus_tag="SAMN05192584_10270"
     CDS             complement(8373..10904)
                     /EC_number="3.4.24.3"
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973110.1"
                     /locus_tag="BM237_RS03110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10270"
                     /product="collagenase"
                     /protein_id="WP_093848075.1"
                     /transl_table=11
                     /translation="MLAAPGHAAPSRVGEKSTEQQAPKDVPPQASVQTEAGDDPEHVRH
                     KPLKVEDRAPLPSTKDALRRDYDDPASLAPRQRPSMQAAPEKKASKKGKASAAAVPGCS
                     TSDFTSRTGSALVQQIKASTTDCINTLFSLTGSDAYHAFREGQMTSVAYALRDGSASYP
                     GNGSTGMPQLVLYLRAGYYVHWYNEATVGTYGPGLQTAIRAGLDAFFANPRSRDVTDAN
                     GEVLAEAVTLIDSAEENARYLYVVKRLLNDYNSTYNSSWWMLTAVNNVYTVLFRGHQVP
                     AFVAAVSSDRSILDTLHNFASSHKNLLGTDQSYLASNAGREHARFLQHTSMQNTVRPQA
                     AGLLNQSSIKGATAPLWVGIAEMTDNYDKANCGYYGTCNLQQRLMADVLVINHTCSASL
                     RIRAQQMTSAQLADTCRSLADQDAYFHSIAKDSGPVAGDRNTSLEVVVYDSSTDYRTYA
                     GATWGIDTNNGGMYLEGNPSAAGNQARFIAYEAEWVRPAFEIWNLNHEYTHYLDGRFNM
                     YGDFNANVATPTIWWIEGFAEYVSYSYRNLRYDAAITEAGKRTYALSTLFDTTYSHDST
                     RVYRWGYLAVRYMLQSHRAEMDTVLGYYRTGNWNAARSYLTGSIGTRYNSDWYSWLSRC
                     AAGDCGGSTNPPGNQAPTASFTVAVSGLTANFTDRSTDSDGTIASRSWNFGDGTTSTAA
                     NPSKTYTAAGTYTVKLTVTDDKGATSTATQSIAVGGADPTPECTGSDTRELDRNCKRSN
                     LTATAGNYAYLYIYLPAGTQQLKITSSGGTGNADLYYSNSTWATTTTYTSRSTGAGSSE
                     TLTITNPAAGWNYISLAAAEDFSGVTVKTEY"
     gene            complement(11243..12424)
                     /locus_tag="BM237_RS03115"
                     /old_locus_tag="SAMN05192584_10271"
     CDS             complement(11243..12424)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003973109.1"
                     /locus_tag="BM237_RS03115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05192584_10271"
                     /product="S1 family peptidase"
                     /protein_id="WP_277347713.1"
                     /transl_table=11
                     /translation="MAALIAAVFLAPPASAGEAVQAQPRLDAQQKAAVTQLAKDYGIPA
                     EEGQRRIARQDEQAALATKLRKRLGARFGGAWVDHDRGGKLMVAVTGKSAVPTVSALAS
                     KAAAEAVVVQRGMSELQKMSEELGKRIAKANKGAEYGLQSAVVTEKNVLRLDLPQGREL
                     TGEQQRTVRWAERKFGDALVIDTYQHASEPLYCGGQYSCDPPLRSGLAIYGGNTRCTSA
                     FMAYAGSSYYMLTAGHCTEAASYWQVPTYSYGYQGVGSTASYHFGYYGDYAAVRISDPA
                     FWQPRGWVYTTQPIRSWNYDYVGQYVCKQGSTTGYTCGQITDTNATVYYPGRTLTGMTW
                     STACVAPGDSGSGVYNGSTAHGILSGGPNSGCGMIHEPISRALSSLGVTLLAG"
     misc_feature    complement(12575..12585)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
ORIGIN
        1 ggtacggaac gacggcccct tggacccctc ccaactcctg ctgttggatg ccggggtgga
       61 gaccgacacc ctctacaccg ccgacatcac ccgtaccctt cccctgtcgg gacgcttctc
      121 ccccgtgcaa cggcaggtct acaacctggt gctggcagcc caggaggccg gcatcgccgc
      181 gttgcggcca ggcgcctcgt tccgcgattt ccaccgcgct gccatgagag tgatcaccga
      241 aggcctgtgc gactggggcg tgctgaagac tcccgccgac gagttgggcg gggacagcgg
      301 cctgcatcgc cgctacaccc tctgcggcag cggccacatg ctcggcatgg acgtgcatga
      361 ctgtgccaag gcccgtgccg gtgcgtacgt tgagggagtg ctgaaggaag gtcaggtcct
      421 gaccgtcgag ccgggtctgt acctgcaacc cgacgatgag accctgccgg ccgaactgcg
      481 gggcatgggc gtgcgcatcg aagatgacct ggtgatcacc aaggacgggg cgcggctgat
      541 gtcctccgcg ctgcccagga ccgcggacgg catcgaggag tggatgggcg cgctgcttca
      601 ggggtgacgg gcccacctgc tgttgggtga ggagcgtgtc cgaccggaca cactcctcac
      661 cctgttcgcg aggctccgcg gactctgcgg ggacagcgcc gactttgcga ggtcagcgtg
      721 cgttcagttt ccgagccggg gacttctgct cgggcgtctc ctgcttgcgt gcggcccaca
      781 gggagcggac gtgcgccagg tggtggcgca tgtgttcctc ggccgccgcg gcgtcaccgg
      841 ccagcatgaa atccagcagc tcggcgtgtt cccgcgcgga ggccaccagc gcgcccgctt
      901 ccgcgagctc ggtgagtccg tagagccggg agcgtttgcg caggtcgccc accgtctcta
      961 cgaggcgtga gttgccggcc agggcgagca ggccgaggtg gaagcggcgg tccgcctcca
     1021 ggtagccgat gaggtcgccg gcgcgcgcgg cggccacgat ctcctcggcc gtggggcgca
     1081 gggcctccaa ctgctcccgc gtggcggacc tggccacctt gccgacggtc gggatctcga
     1141 tcagggcgcg gatctcggtg aactcgtcca ggtcgcgctc ggacaactcg gtgacacgga
     1201 agcccttgtt gcgcaccgat tcgaccagcc cctcgtgcgc caggtccagc atggcctcac
     1261 ggacgggagt ggcggagacg ccgtactcgg cggcaagggt gggggcggag tagatcaccc
     1321 cgggccgcag ttcgcccgcg atcagcgcgg cccggagggc gttggccacc tggtcgcgca
     1381 agtgctcttg caccgaaacg agtttccggg gtttcagctc gctcatgggt tcccctccgt
     1441 gggtttgtga tcccactata caatgtcacg ttgcgcggta cttgcgccat ccccggcgcg
     1501 accggccagg tcgcgccggg gatcgggagg ggacccgtct cgcggcgttt caggctgatg
     1561 cggcgggcag gagggacact gtgcggctgg aggtgtagaa gtccatcgcg gcgtgtccct
     1621 gttccctcat tccgtaactg gagtccttct ctcccccgaa ggggaggtgg aagtcgacac
     1681 cggtggtcgg agcgttcacc cgcagcatgc ccgcggtgag gcggtcgacc gccgagagcg
     1741 ctttcgccag gtcacccgtg tgcagcgacg cgaccaggcc gtagtgaccg ctgttcgcgg
     1801 cctggaccgc ttcgtcgaag tccgctgccc ccgacagcac cgcgatgggg ccgaagatct
     1861 cacggcggtg cagctcgtgt gcggcgggca cgttctccag cagcaccgga tcgacgtacc
     1921 agcccgctgc tgcgcccgaa ccgcgccgac cgccccctgc tagcgcacgt gccccggtgc
     1981 tgagggcgct gtcccgggct cggacgacac ggtcgcgggc cgctttggag atgacgggac
     2041 cgcagaccgc ggccgccgcg tgggggtcgc gggcgggcag ctcccgcagg gcctccgcca
     2101 gcgcctcgcg cagtttcggc agagcgctgc ccactgcgat cactcttctg gtggcggtgc
     2161 acttctgccc cgcgtaaccg gcgatcgccg cggcgatgtc cgcagccgcc ttccctatgt
     2221 tggcgtccgg caggaccacg gcagcgttgt ggccgcccat ttccgcctgg gcggggatgc
     2281 cgcgggctgc ggcggcacgg gccaccacag cgccgacggc ggtcgagccg gtgaaggaga
     2341 cgacatcggc caggccgatc agggcggcgc cggcttcggc gcctccggga acgacctgca
     2401 gaacgtctgc gggcaggacc tccttgagga tctcggccag ccgcagagcg cacgcggtcg
     2461 cctccggtgc gggtttgagg acgacggcat tgccggcggc cagggccgga gcggccttcc
     2521 aggacggaat ggccagcggg aagttccacg gggtgatcag gccggcgacg ccgtgcgggc
     2581 gccgccgggt gagcagcagc cccggccccg aggccggctc gtgcaccgcc ccggtggcgt
     2641 cgtagggaag ctgagcgtag tagcgccaga tcgccgccgt gcgcgccagc tcgccgcggg
     2701 cctcggcgag cggcttgccg acttccctca ccaggagtcc ggccagttcc tcggacgcga
     2761 tctccacggc gtctccggca cggcgcaggg ctgcggatcg cgcggccgcg ccggcagcga
     2821 gccactcgct ctgggctcgg gcagcccgcc gcacggcacg ggtagcggtc tcggggccgc
     2881 agccgtacaa ctcgatgagg atgtcgctgg ggtcggcggg gttgcgggag atcaccgggc
     2941 tgtcggccgc cggggcggga ggggtgttca cagcaggaag ccttccggga acgggtctgt
     3001 gggatcgagg aagaactggg cggtaccggt gatccaggcg cttccggtga acgtcggcac
     3061 cacggccgcc ctgccggcga cctcggtctc ctccaccagg cggccggtga aggaggtgcc
     3121 caggaaggac tcgttgacga aatcgcggtg cagaggaagt tccccccggg cgtggagctg
     3181 cgccattcgc gccgaggtgc cggtgccgca gggcgaccgg tcgaaccatc cggggtggat
     3241 ggccatcgcg ttccgtgatc gtcgggcatc cgaccccggg gcgaggagct ggacgtgatg
     3301 gcatcccgtg atccctggat cctccgggtg caccggttcg cctgcggtgt tgatcgcagc
     3361 catcacctcc aggcccgcac gcaggagttt ctccttgcgt gaccgatcga agggcagtcc
     3421 cagttcctcg acggggagga tggcgtagaa gttgccgcca taggccaggt cgtaggtgat
     3481 ccgcccgaag ccgggaactt cgaccttctc ccccagggca acggcgaaag aggggacgtt
     3541 gcgcagtgtc actgcgcggg cggcgccgtc gtggacgtgg acctcggcgg tgacgagccc
     3601 ggccggtgtg tccagccgga cggtggtcac cggctctgtc accggtacca tgccggtctc
     3661 caccaggacg gtggcgaccc cgatggtgcc gtgcccgcac atgggcaggc agccggacac
     3721 ctcgatgtag atgacccccc agtccgcgtc ggggcgggtc ggaggctgca ggatcgcccc
     3781 gctcatcgcc gcgtgcccgc gcggttcgta catcagcagg gtgcggatgt ggtccaggtt
     3841 gcgcatgaag tacgtgcgtc gttcggccat ggtggccccg gggagcggtg cgattcctcc
     3901 ggtgatgacg cgcgtgggca tgccctcggt atgggagtcg acggcgtgga agacacggcg
     3961 actacgcatg gctgttctcc tgcggactgg ggtgtcgggg gtgcgggacg gatcagttca
     4021 gaccctcggc caggaccttc tccgtcacgg atcgcacggc ggcctccgcc tctgtgtcca
     4081 gcggggagcg cggcgggcgg caggggccgc cgtgtcgtcc ggcgatgtcc atcgagagct
     4141 tgatggcctg cacgaacacg tgcttggtgt cccagcgcag cagcgggtgc agcgctctgt
     4201 agaggggcag tgcggtctcc aggtcctggg cgatggcggc ccggtacagt gcgacgcagg
     4261 agtggggcag ggcgttgggg tagccggcga tccagccgac ggcgccggcc agggccagtt
     4321 ccagcaggac atcgtcggcg ccgatgagca ggtccaggcc gggggcgcac tcagcgatct
     4381 cgtaggcccg tcgcacgtcg ccgctgaact ccttgaccgc gacgatgtgc tgttgcgcgt
     4441 gcagatcggc caggagctgc ggggtgaggt ggacacgggt gtcgtagggg ttgttgtagg
     4501 ccaccacggg cagcccgaca cccgcgacct cggcgtagtg agcgcggacc tccgcgtcgt
     4561 tggcgcggta ggcgttgggg ggcagcagca ggacggaccc ggcgccggcc tcggcagcct
     4621 gctcagtcca gcgcgcggcc tcggtgctgc cgtaggcggc cgctccggcc atgacccggc
     4681 tgccgtcccc ggcggcctcc accgcggtgc ggatgacgcg ggcgcgttcg tcgtcggtga
     4741 gggtctggta ctcccccagg gagccgttgg ggacgacgcc gtcgcagccg gcgtccagca
     4801 ggaagcggac gtgttcggcg taggcgtcgt agtccaccga caggtcttcc ctgaagggaa
     4861 gggcggtggc caccatgaca ccgcgccagg ggtgttcggg gctgtgctgg gatgcgctca
     4921 cgttcagttc tccttgggtt gggcgaatcc ggccgggccg gttttcgagg gggtgggagg
     4981 ttctggtcgg gttgcgccgg tcaggggcgc ccctgggggc ggtgcctggc gagctggacg
     5041 agggggacgg ggcgggacag ggggcgccga tccgcgctca ccggtgcgct gccgtcgccg
     5101 gacacgaggc aggagaccgc ggggccgcac atccgtccct gacaccagcc catgccggcc
     5161 cgggtcagca gcttggccgt gcgcgtgtcg cccgcgccga aggtgtcgac cgcctcgcgc
     5221 acggatgatg cgtcgatttc ctcgcagcgg cagatctcgg tgtcgcctcg cagccactgg
     5281 ggccaggccg gtccgggccg gtgaacccgc gccatgaggt cggcgaaggc ccgcagccga
     5341 gtggcgcgtg tgcgcagcgc cgcagccgtg cgcgcggcag tggtttcgcc gtgcgctgcg
     5401 gcagcgacag catgggcggc cagctctccc tcgcacaggg cgagctgggc gccgccgacg
     5461 ccgttggtct cgcctgccgc ccatacgcct tcgacgctgg tgcgcaggaa ggagtccacc
     5521 acgagggcgg gtgtgccgtc gggtgcggtg cgggtagcgc agcccagttc cacggcgagt
     5581 tcgagctggg gtaccagacc gtggcctacc gccagggcgt cgcaggacag gtgttgctcg
     5641 gtcccgggca gcggccgcca gtcgccgtcc agcctcgcca cggtcacgcc ggtgacccgg
     5701 tcggtcccat gggcttggac cacggcggtc cgggtgcgca gccctaccct ccggcgggcg
     5761 agcgccgccg cgtgccgggc tccttcggcg agcttgccga ggttgcgcag cagtggtccc
     5821 ggtcggcggg cgtagggcag gtagctgctc gcctccagga cgtatggcac gcgggccccg
     5881 gcggcggaca gcgaggcggc cgcggccagt agcagggggc cgcttccggc tacgatcacg
     5941 cgccggcccg gcaggaccag gccggacttg atcatggcct gggctcctcc ggcgccgacg
     6001 actccgggca gtgtccagcc ggggaagggc agctggcgct cgtaggttcc ggtcgccagc
     6061 agcaggtggt gcgccggcac gctctctccc ggtgcctcgc cggtgttctg gtcgccaggc
     6121 gcggcccggt cgtgcggtgg cacggtcgcg gtggtgagca gtgtccagcg gcccgcgtgg
     6181 tcgcatgatt gttggatggt ccacacctgt cgtcccggca ggtggcggac ccggccgacc
     6241 tgcttgtgct gacgcagccg ggtctcgagg gtggtgaagg cgggccagcc gtggtggagg
     6301 gcctcggggc gtcgtgctcc caggccgggc gcggggtggc ggtagtactg gccgcccggc
     6361 ctgggttcgg cgtccaccag ggctacggtc agccccgcat cggccgcggt gacggccgcg
     6421 gcgagtccgg cgggtccggc gcccacgact gccaggtcgt acggggctcc ggagggctca
     6481 gcaggcgagg tcggcacggc cgtctccttc ctggggggtg acggtgtcgc cgtcgtgcgc
     6541 cggcaccagg cacgcgcgct ggttggggcg gccgttgacg gtgatcaggc agtcgaagca
     6601 gactcccatc ccgcagaagg caccgcgggg gcgcccgccg cggcgggtgg tgcgccagga
     6661 gacgatgccc gcggcccaca gggcagcggc gatgctctgg ccgggcagtg ccgggagctt
     6721 tctgccgtcg tagtcgatgc tgatggcgga gtggggttca gcccgcacca gcccggccgg
     6781 ggtgcgtgac atgggacctc cctggggttt cggcgggcgg tggtcgccgg tgcggtgccg
     6841 cgtgctcatg cgtgctggac ctctgattcg aagcggtcgg ggcggaaggg ggcgaggtcc
     6901 agcgagggct tttcccccgt cagggcctgt gcgagcagca gccccgtggc cggtgccagc
     6961 ccgatccccg cgccctcatg cccgcaggcg tggtggaggc cggggacgcg ggggtcggcg
     7021 ccgatcgccg ggaggtggtc gggcaggtag ggccggaaac cgtggtaggc gcgcaggacg
     7081 gagacctggg ccaggatggg gaagagggtc accgcgctgt cagccatgcg ccgcagggcc
     7141 tcggtggaca gggtcctgtc gaatccggtg cgctcccggc tggacccgat cagtacgggg
     7201 ccggccggtg tgccctcgac cacgggggaa gtctggaggg aggcggagtc gctggcgacg
     7261 tcggcgacgt aggcggcggc gtagaccttg tgtcgcacca cgcggggcag gggttcggtc
     7321 accaggacga agcccttgcg cggcatgacc ggcagggtcg tcccggccag cgcggcgatc
     7381 tgcgctgacc aggtgccggt ggcgttgacc accaccgggg ccttcagctc gcggtggggg
     7441 gtgcgcactc cgtcgatccg ccccgcgggg gttaccagca cctccgtgac cgcctcccgc
     7501 aagtggaggg cggcgcccga gttctgggcg gcgcgcagca ggcgggacgc ggcgagggcg
     7561 ggctgcacct gggcgtcctg ggggtagtgg acggccccgg ccaggccggg ggccaggtgc
     7621 ggctcgaggt ccggcagccg gtcggcgggc acctctcgga ccacgacacc ggctgcggcc
     7681 tgcccggcgg ccatgcggcg cagcgcggac cactcggtgt cgtccgcggc caccacgact
     7741 ccgcccttgg cctcgtattc gatcccttcg ggcaactggg cggccaggtc gcgccacagg
     7801 cccgcagaaa gctgggcgag atcgagctcg gggcccgggg gcttgtccga gaccagcagg
     7861 ttcccctccc ccgcccccgt cgtaccaccg gccgggctgc cgcggtcgac gacggcgacg
     7921 gcgagtccgg cgcgggtggc gtagtaggcg cacgcggcac cgacgactcc cgcgccgatg
     7981 acgacggcat cgacggtctg tctcatgagc accacagtaa tatgtcacat gcttctgcga
     8041 tagcccggga ggcaccgttt tcctttcgga agggaacgca gaagccgggg cgggcgcgag
     8101 ggattcctca cgcccgcccc ggcgatcacc ggaaggcgga gcgggccggt gccacctgag
     8161 cggctacctg gacaggaccg agactggtcg ggttgcgcgg cgacgggagg cgtcgccccg
     8221 ctgttccggc tccgcgcctc ttaggcgcgg taatactatg tcacacctca ttgatgtgcg
     8281 tggtgctctg cccgggccct gtggcaaggc cacggtgccg ggacgggcgg ggttccttcg
     8341 cccgcccggc ggtcggcgag gaggcggagg ggtcagtact cggtcttgac ggtcaccccg
     8401 gagaagtctt ccgcggcggc caggctgatg tagttccagc cggccgccgg gttggtaatg
     8461 gtgagcgtct cgctgctgcc cgcgcccgtg gaccggctgg tgtaggtggt ggtggtcgcc
     8521 caggtgctgt tgctgtagta gaggtcggcg ttgccggtcc cgccggatga ggtgatcttc
     8581 agttgctggg tgccggcggg gaggtagatg tagaggtagg cgtagttccc ggcggtcgcg
     8641 gtcaggttgc tgcgcttgca gttgcggtcg agttcccggg tgtcgctgcc ggtgcactcg
     8701 ggagtggggt ccgcgccacc gacggcgatg ctctgggtgg cggtgctggt ggcgcccttg
     8761 tcgtcggtga cggtcagctt cacggtgtag gtgcccgccg ccgtgtaggt cttggagggg
     8821 ttggcggcgg tggaggtggt gccgtcgccg aagttccacg agcgcgaagc gatggtgccg
     8881 tcggagtccg tggagcggtc ggtgaagttc gccgtcagac cgctgacggc gacggtgaag
     8941 gacgcggtgg gcgcctggtt gccgggcggg ttggtggagc cgccgcagtc gccggctgca
     9001 caccgcgaca gccagctgta ccagtcgctg ttgtagcgtg tgccgatgct gccggtcagg
     9061 taggagcggg ccgcgttcca gttgccggtg cggtaatagc ccaggacggt gtccatctcg
     9121 gcgcggtggg actggagcat gtagcgcacg gcgaggtagc cccagcggta gacgcgcgtg
     9181 gagtcgtggc tgtaggtggt gtcgaacagg gtgctcagcg cgtaggtgcg cttgcccgcc
     9241 tcggtgatgg ccgcgtcgta gcgcaggttg cggtaggagt aggacacgta ctcggcgaag
     9301 ccttcgatcc accagatggt gggggtggcg acgttggcgt tgaagtcgcc gtacatgttg
     9361 aagcggccgt cgaggtagtg cgtgtactcg tggttgaggt tccagatctc gaaggccgga
     9421 cgcacccact cggcctcgta ggcgatgaag cgggcctggt tgccggcggc ggaggggttg
     9481 ccctccagat acatgccgcc gttgttggtg tcgatgcccc aggtcgcccc ggcgtaggtc
     9541 cgatagtcgg tgctgctgtc gtagacgacc acctcgaggc tggtgttgcg gtcgccggcg
     9601 accgggccgc tgtccttggc gatgctgtgg aagtaggcgt cctggtcggc caggctgcgg
     9661 caggtgtccg ccagctgcgc cgaggtcatc tgctgtgcgc ggatgcggag actggcactg
     9721 caggtgtggt tgatcaccag gacgtcggcc atcaggcgct gctggaggtt gcaggtgccg
     9781 tagtagccgc agttggcctt gtcgtagttg tcggtcatct cggcgatgcc gacccacagc
     9841 ggggcggtgg ctcccttgat ggagctctgg ttcagcagcc cggccgcctg cggccggacc
     9901 gtgttctgca tcgaggtgtg ctgcaggaac cgggcgtgct ctcgcccggc gttggaagcc
     9961 agatagctct ggtcggtacc caggaggttc ttgtgggagg aggcgaagtt gtgcagcgtg
    10021 tccaggatgc tgcggtcaga ggacaccgcg gcgacgaagg cgggcacctg gtgaccgcgg
    10081 aagaggacgg tgtagacgtt gttgaccgcg gtgagcatcc accaggagga gttgtaggtg
    10141 ctgttgtagt cgttcagcag ccgcttgacg acgtacaggt agcgggcgtt ctcctcggcg
    10201 ctgtcgatca aggtgacggc ctcggcgagc acttcaccgt tggcgtcggt gacgtcccgg
    10261 gagcgggggt tggcgaagaa cgcgtcgagt ccggcgcgga tggcggtctg caggcccggt
    10321 ccgtaggtgc ccacggtggc ctcgttgtac cagtgcacgt agtagccggc gcgcaggtag
    10381 agcacgagct gcggcatccc ggtactgccg ttgccggggt aggaggcgga gccgtcgcgc
    10441 agcgcgtagg cgacgctcgt catctggccc tcgcggaagg cgtggtaggc gtcgcttccg
    10501 gtgaggctga acagagtgtt gatgcagtcg gtcgtggatg ccttgatctg ctgcaccaga
    10561 gcactgccgg tgcggctggt gaagtcgctc gtgctgcagc cgggcaccgc tgccgccgag
    10621 gccttcccct tcttggaggc cttcttctcc ggggccgcct gcatggacgg cctctgccgc
    10681 ggcgcgaggg aggcggggtc atcgtagtcc cggcgcagcg cgtccttcgt cgagggcagc
    10741 ggggcgcggt cctcgacctt cagcggcttg tggcgaacgt gttcgggatc gtcgcccgcc
    10801 tctgtctgca ccgaagcctg aggcggtacg tccttggggg cctgttgttc ggttgacttc
    10861 tcacctacgc ggctcggggc ggcgtgtccc ggagcggcga gcatcccgat cccgaggcac
    10921 agcgccagga tcagcacact cagcctcgcg agagggagtc gggcattttc tgttctcact
    10981 cgctgcctcc agcgggagga agggccgtat gggggcacgg ccgggtgggg tgtccacacg
    11041 acgggcccgg aggagcagcg gggaggcaca ctcccccggg ttgtcatggg cacatctata
    11101 agatgtgaca ggtaatattg cacatgtcac ttcgggtggg aagagtccga agtgaaggaa
    11161 tggaaagcgc gcctggagtt cggcgcctcc ggcgaggagc cgccgcgctc cctctgcgcc
    11221 gagcgcggcg gcctctgccc ggtcagccgg ccaggagcgt gactcccaga gaggagagcg
    11281 cgcgactgat cggctcgtgg atcatgccgc agccgctgtt cgggccgccg ctgagaatgc
    11341 cgtgcgcggt ggatccgttg tagacaccgc taccgctgtc gcccggcgcc acgcacgccg
    11401 tgctccaggt catcccggtc agggtgcggc cggggtagta gacggtggca ttggtgtcgg
    11461 tgatctgccc gcaggtgtag ccggtggtgg agccctgctt gcacacgtac tggcccacgt
    11521 agtcgtagtt ccagctccgg atgggctggg tggtgtagac ccacccccgc ggctgccaga
    11581 aggcaggatc actgatccgc acggcggcgt agtcgccgta gtagccgaag tggtaggacg
    11641 cggtgctgcc cacaccctgg tagccatagc tgtaggtcgg gacctgccag taggaggcgg
    11701 cctcggtgca gtgcccggcg gtcagcatgt agtagctgga accggcgtag gccatgaagg
    11761 cgctggtgca gcgggtgttg ccgccataga tggccagccc cgagcgcagc ggcgggtcac
    11821 aggagtactg cccgccgcag tacagcggct cggaggcgtg ctggtaggtg tcgatgacca
    11881 gtgcgtcccc gaacttccgc tcggcccagc gcacggtccg ctgctgctcc cccgtgagct
    11941 ccctcccctg cggaaggtcc agtcgcagca cgttcttctc ggtcaccacc gcggactgca
    12001 ggccgtactc ggcgcccttg ttggccttgg cgatccgctt gcccagctct tccgacatct
    12061 tctgcagttc gctcatgccg cgctggacga ccacggcctc ggccgccgcc ttcgaggcaa
    12121 gagcgctcac cgtcggcacc gccgacttcc ccgtcaccgc gaccatcagc ttgccgcctc
    12181 ggtcgtggtc cacccacgcc ccgccgaaac gcgctcccag tctcttgcgc agcttggtgg
    12241 cgagagcggc ctgctcgtcc tgccgcgcta tgcgccgctg cccttcctcg gccgggatgc
    12301 cgtaatcctt ggccagctgg gtcactgcgg ccttctgctg ggcgtccagg cgcggttggg
    12361 cctggaccgc ctcaccggcc gaagccggcg gagccaggaa gaccgcggcg atgagagcag
    12421 ccaaagccgc ccacaccgcc cccgacaacc gtagtgaagc accgtgcatc gagcacctcc
    12481 gtgtgatgga gcgaacaccg gatgcagtgg acacctcacg cagcgcgtcg atgaacatcc
    12541 ggcgtcagga gcatgacagg gaacaatatg gcgggtgaca tttcacatat caagggtggt
    12601 gtttcaatct ccgcagaacc actcgctgtg cgtaggcaaa ccccctggtc agcgagacca
    12661 gttctcctgc gactcgcctc aacaaactgc tacatggctg gtgaagcttc cccttgatcg
    12721 tggacacctg gagactggga cctgaggttc caccagagga agtagcacca ggtgggaggc
    12781 aagtacacga agcggtacac cgaggagttc aagcgggacg cgatcgcgct cgtcgactcc
    12841 tcgggcaaga cggtcaccgc cgtcgcccgg gaactcggca tcagctccga atccctgcgc
    12901 ggctggtacc gccgggccaa ggccgaccgg gccgagggtg ccccgggcga gttgaccggc
    12961 gtgagcgtga ggaactgcgc cggctgcgca gggagaaccg taaccgccgc cg
//
