LOCUS       NZ_FOZB01000001        12241 bp    DNA     linear   CON 28-FEB-2024
DEFINITION  Pseudobutyrivibrio sp. NOR37, whole genome shotgun sequence.
ACCESSION   NZ_FOZB01000001
VERSION     NZ_FOZB01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Pseudobutyrivibrio sp. NOR37
  ORGANISM  Pseudobutyrivibrio sp. NOR37
            Bacteria; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae;
            Pseudobutyrivibrio.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/28/2024 14:17:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,726
            CDSs (total)                      :: 2,671
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 4, 3 (16S, 23S)
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 3 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            Pseudo Genes (multiple problems)  :: 2 of 9
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:29:30
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1120832
            Orig. end                         :: 1133073
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOZB01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12241
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12241
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12241
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7241](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..7241)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(334..639)
                     /locus_tag="BM306_RS05250"
                     /old_locus_tag="SAMN04487829_1049"
     CDS             complement(334..639)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607664.1"
                     /locus_tag="BM306_RS05250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487829_1049"
                     /product="hypothetical protein"
                     /protein_id="WP_090487319.1"
                     /transl_table=11
                     /translation="MTLLLTVFAAIITTVKWYNRENDNMKLHVLMYMFWGASLMWFVDA
                     IAEYIELGAEYFNPALEDMINDSFLGLSVIAFALIIWVVYLLVKDPKGVVRKSITK"
     gene            complement(650..1033)
                     /locus_tag="BM306_RS05255"
                     /old_locus_tag="SAMN04487829_1050"
     CDS             complement(650..1033)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549485.1"
                     /locus_tag="BM306_RS05255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487829_1050"
                     /product="hypothetical protein"
                     /protein_id="WP_090155218.1"
                     /transl_table=11
                     /translation="MACFTVPAGEAIVSTIVKKSIEKKNKGGEKAAESTGIPFSRKLKW
                     LTNLLWGGAVLLAYEHVWHGEVKPFFPFLTAMENPEDKAEMLHEMGTVGVGMALLVTVV
                     WIGICVAADVIVKRPVANTEAAK"
     gene            complement(1157..2071)
                     /locus_tag="BM306_RS05260"
                     /old_locus_tag="SAMN04487829_1051"
     CDS             complement(1157..2071)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426272.1"
                     /locus_tag="BM306_RS05260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487829_1051"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_090487322.1"
                     /transl_table=11
                     /translation="MTLKQLRYVVEVAETGNITEAAKKLFIAQPSLTSAIRELENEYNI
                     TIFTRSNKGIEITPEGDEFLGYARQVLDQANLIEEKYTGVSHIKQRFCVSAQHYSFVVE
                     AFVELLKKYGGDKYEFHMRETQTYDIIEDVAKLRSEIGILYINKFNETVIKKTLRDNGL
                     NFHLLFKAKPHVFIGKNSPLAKKKSLSLEDLKEYPRLSYEQGSHNSFYFSEEILSTIDS
                     DKEIIVRDRATLFNLLIGLNGYTICSGVISEELNGANIIAKPLKVDDYMEIGYILPSSI
                     PQSQLTKNYIEMLVKYTAEGKNN"
     misc_feature    complement(2192..2212)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 23.89"
                     /tool="antismash"
     gene            2268..3386
                     /locus_tag="BM306_RS05265"
                     /old_locus_tag="SAMN04487829_1052"
     CDS             2268..3386
                     /EC_number="2.1.1.14"
                     /GO_function="GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391103.1"
                     /locus_tag="BM306_RS05265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487829_1052"
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="WP_090487326.1"
                     /transl_table=11
                     /translation="MSKIQTPYRYDFVGSFLRPQALKDAKAAFQAGKITKEEFDKVVNE
                     EITKVVAKQKKLGYHVITDGEFRRTFWHLDFMWGFEGVAHENTGGGVSFNGELALLDDT
                     YLVGKIKAKAHPFVEYFKFLKQFEDENTVAKYTIPAPAQTFQQMIVPANYETTRKFYPT
                     NEELIQDIGVAYQDVIKQFYEAGCRNLQFDDCTWGAIVGDAAKQRYASLGIDLEDVKKQ
                     LLEVNNLALENKPADMVITSHICRGNYHSTFFTSGPYDSVADYVFAKENVDALFLEYDD
                     ARSGGFAPLAKVSPDKKVVLGLITTKTPQLENKETIIKRIKDASKYIPLDRLYLSPQCG
                     FASCEIGNKLTEDEQWAKLKLVKEIAEEVWGK"
     gene            complement(3451..5004)
                     /locus_tag="BM306_RS05270"
                     /old_locus_tag="SAMN04487829_1053"
     CDS             complement(3451..5004)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /locus_tag="BM306_RS05270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487829_1053"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_090487329.1"
                     /transl_table=11
                     /translation="MKPVFIDIGEISESVEVYESKPNPAIVYTIYTIFLMLVISVIWTV
                     NFKLDNVVKSNGVFKGTDSIFEVSSAVTGCVKEANVEDGQFVEKGDVLFVVNIESLSET
                     IQNYQKEKTDAENRLEILDAYEQSLDTGAGIPDSLKDNPYYDEFVNKRKLLLENISVSS
                     EDLEGKISIYQGSIDNTNETIAVYTEKVEKLTEVKNCILNRENTFQPEETYYYSMVNSY
                     LASYNYNRVQYNNKISEYNNQIDTCNSQISEAEDDEEKEALYEQRNALYSSVENMENEL
                     TKALSSLETQQISEIESQIESINNTLLSLNSNLSETQLELNANTTGTAESVKKIAILTE
                     KGNIYTEELTYNEKKDECENYLTSYGIQNDNCTIRAASSGYFYSTEDVKVGTYLNESTT
                     LGKIYPKEESDFYAQIYVENSDIGKIEEGQNVKFEIAAFPSREYGYFEGIVSSISKDIT
                     VDQTTGEAYYIVKVACKNMMLSGKDGTEASLKNGMACTAKIIVGERSVINYIIEKLHLK
                     "
     gene            complement(5001..7241)
                     /locus_tag="BM306_RS05275"
                     /old_locus_tag="SAMN04487829_1054"
     CDS             complement(5001..7241)
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470428.1"
                     /locus_tag="BM306_RS05275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487829_1054"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_090487332.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.4e-36, bitscore:
                     121.4, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 1.2e-213, bitscore:
                     707.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKYYCVKQHDITDCGAACLATICKQNGYKLSISKIREIAGTDKKG
                     TNAFGVIKAAEELGFSAKGVKGTQEGFFSEIPLPCIAHVIVDGKLLHYVVIHKITKKQV
                     TIADPAKGLIKISPEEFFGEVHEDNKPPTYQWSGVLILLVKKETFVKGDETKGLFSRFF
                     YLLVPQKKLLVHIFIASLVYTILGILGAFYFQEMIDSVLPDALSKTLMTLSIGVIALNL
                     FRVVLNAFRTHLLLYLSQKLDIAMLLGYYQHILELPMNFFGTRRVGEIISRFNDAGKVR
                     DAISGATLTIMIDTLMAIAGGIILYMQNAKLFGITAIIVVLYFVIVLAFNGLYERLNRK
                     QMEDNSQLTSYMVESLNGIQTVKSYNAERKVNHETEKKFVKLLKSIFNLTWVGNIQTSL
                     KTIIELVGGVVILWVGGLSVIKGEMTIGGLITFNSLLAYFLDPVKNLINLQPQMQTAIV
                     AADRLSEILDLEAEKKEGESRKLQPKRIAGDIEMKNIKFRYGTRRLVIDDVSLNIKQGQ
                     RVAFVGESGSGKTTLSKLLLNLYQPEEGEILINKNNIKDIQIEKLREKIAYIPQETFLF
                     SGTIYENLILGLDDVNMDDVIEATKMAQAHDFINDLPLRYETMLEENGSNLSGGQRQRL
                     AIARAMLKKPDILILDEATSNLDAVTERALDETINKFAKDLTTIFIAHRLSTIKNCDVI
                     YVMEKGKVIESGNHEELQRLGGKYAELVRQQSLGEITISENIKELKEEGEVS"
     misc_feature    7563..7578
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.98"
                     /tool="antismash"
     misc_feature    7664..7678
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 23.46"
                     /tool="antismash"
     gene            complement(7885..8247)
                     /locus_tag="BM306_RS05290"
                     /old_locus_tag="SAMN04487829_1056"
     CDS             complement(7885..8247)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM306_RS05290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04487829_1056"
                     /product="hypothetical protein"
                     /protein_id="WP_143096638.1"
                     /transl_table=11
                     /translation="MIDGGKLVNPLFLSVIVLLDLFFVFFMTLFFYVVAKPSSITFTVK
                     DRRGLINELSEVASKKWKRNNKVETKETCSFKFGNKYKDWLTTPIDFIFEEEKCTVIVP
                     SYYQEDVLRINKKLRE"
     gene            complement(8633..9010)
                     /locus_tag="BM306_RS05295"
                     /old_locus_tag="SAMN04487829_1057"
     CDS             complement(8633..9010)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM306_RS05295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04487829_1057"
                     /product="hypothetical protein"
                     /protein_id="WP_090487341.1"
                     /transl_table=11
                     /translation="MKLQWKVNFGLLIIYFTAVSISAAYNAVIIKDLEPIFYVRNILCI
                     GVAVVTTVKILVNRNLLESSLVDKLCRWIAVFRILLLFGALYIVITENIVASNNLGSVG
                     SIIAAMVMVDVDINYRKKTVR"
     gene            complement(9031..9216)
                     /locus_tag="BM306_RS05300"
                     /old_locus_tag="SAMN04487829_1058"
     CDS             complement(9031..9216)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM306_RS05300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04487829_1058"
                     /product="hypothetical protein"
                     /protein_id="WP_090487345.1"
                     /transl_table=11
                     /translation="MRELTNNELQIIDGGVNGLKVAGGVLLVAAGVGECATVAFSAGGV
                     CTALGGVGCIIDGLND"
     gene            complement(9235..9438)
                     /locus_tag="BM306_RS05305"
                     /old_locus_tag="SAMN04487829_1059"
     CDS             complement(9235..9438)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM306_RS05305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04487829_1059"
                     /product="hypothetical protein"
                     /protein_id="WP_090487348.1"
                     /transl_table=11
                     /translation="MDLVLSNDFSQLSTMDEMLVEGGDWAEFGYAALGTVSIAAAPIAL
                     AAGQPWAAVALVGNGLYQFGNL"
     gene            complement(9688..10932)
                     /locus_tag="BM306_RS05310"
                     /old_locus_tag="SAMN04487829_1060"
     CDS             complement(9688..10932)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /locus_tag="BM306_RS05310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487829_1060"
                     /product="ATP-binding protein"
                     /protein_id="WP_090487352.1"
                     /transl_table=11
                     /translation="MAKLIFIILLLGQFIWAYVKLLDIKFKKMKVTYWIVVGLIELATI
                     LIGIIYNFGPTKISLLAGLFLLPAFNGEKRTNIGLYYPIFGLCISLNTVWGLLSYYFAL
                     EMHYRIFACVSPTWGLIILAYFLKKKKDFLWNDLNFTNVELIIINIFTAFIAISMGAVA
                     NIQSGNNGAYIITIYIVFTTILCSVIYLLTIYSIFLNRKIQNEEKKLLYQKVTLEAQKE
                     QVDAILESEKKYHKYRHDLAAHLNAIHHMAEKSRIDELKEYCETLLDNAKPINNTAISG
                     NVAVDGILRSYKDKCEENGIGLQMQVQLLGKNKVQDYDLCIIFSNILSNAIEACKKGDS
                     IRLVSYPYNNSLCIMANNPTNHEIMINNGEIKTTKNDKTYHGHGLSNIKAVVNKYNGIL
                     NIKAQNGMFNIEILI"
     gene            complement(10920..11651)
                     /locus_tag="BM306_RS05315"
                     /old_locus_tag="SAMN04487829_1061"
     CDS             complement(10920..11651)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="BM306_RS05315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487829_1061"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_090487355.1"
                     /transl_table=11
                     /translation="MESNFLVAVCDDDKENRKAIIDIVLKHFESIGIKAEIKEYKSGED
                     LLKNVNEQICLLFLDIEMPGKSGIEVKDILTTKMNVSNIVFVTNHIGFMKEAFGKNVIG
                     FMEKPIDENQIIHWVNIAYEAFKDTRYINIDKNAKIKEDSIKYIEAEGNYVRIVLVDRS
                     GGILQRGTIANWEKRLDANFVRCHKSFLVNMNYITSIHYKEVELEGNTCVPLGRNYVND
                     TKNAHNEFMLEKIKWRVGWQN"
ORIGIN
        1 tatcgaatat gagtcaacta acgatgaccc tactattgat tatttattaa ttaaaactga
       61 tgaatttaca tcaaacggca ttaaaatcgg tgcttctgaa gaagaagtaa aaaaagcatt
      121 tggcgagcca tctactgaat atgattgtga agaccagcac ttctttgatt attactatga
      181 tgaattctca cagtacttta ctttcaaaga tggtaaactt gtagaattct ctttcacagc
      241 caactgtact accgaataaa cctgttaaca ccctacccct aattaacata aaagggaggc
      301 cataaggcct cccgattttt tttgcaattt taattattta gtaattgatt ttctaactac
      361 accctttggg tcttttacta aaagataaac aacccaaatg ataagagcaa aggcgattac
      421 tgataagcca agaaagctat cgttaatcat atcttctaat gcaggattga aatactctgc
      481 gccaagctca atgtactctg caatagcatc tacaaaccac ataagtgatg cgccccagaa
      541 catgtacatt aaaacatgaa gcttcatatt gtcattctct ctgttgtacc atttaactgt
      601 tgtaataata gcagcgaaaa ctgtaagtaa taaagtcatc ttctacctcc tactttgcag
      661 cttctgtatt tgcaactgga cgcttaacaa tcacatctgc agctacacaa ataccaatcc
      721 aaacaacagt aacaagaagt gccataccaa cacctactgt gcccatctcg tgaagcatct
      781 ctgccttgtc ctctggattc tccatagctg ttaaaaatgg gaagaatggc ttaacctcac
      841 catgccatac gtgctcatat gccaaaagca cagcaccacc ccaaagtaag tttgttaacc
      901 actttaactt acgagaaaat ggaatacctg ttgattctgc agccttttcg ccacctttat
      961 tctttttttc aatacttttc ttaactatag ttgaaactat agcctcgcca gctggcactg
     1021 taaaacaagc cataatagac ctccttatta atcatgaatt cgaatgaaat tatatcaata
     1081 ttataattaa atatgaacgt ctttaagagc ttatttctat tctaattgtg acagcatcta
     1141 ttcctctatg gaatatctaa ttatttttcc cttcagcagt gtattttaca agcatttcga
     1201 tatagttttt tgtcaactgt gactgtggga tactgcttgg aagaatgtaa ccaatttcca
     1261 tgtaatcatc tacctttagg ggtttagcaa taatatttgc gccattaagc tcctcactta
     1321 taacaccgct acagatagtg tatccgttaa gaccaatcaa aagattaaaa agggtagctc
     1381 tatctcttac gattatctcc ttatcactat cgatagtgct aagtatctcc tcagagaaat
     1441 aaaaggagtt atgacttccc tgctcatatg aaagacgtgg atattccttt aaatcctcta
     1501 aacttaatga tttctttttg gccaaagggc tattttttcc tatgaataca tgaggcttgg
     1561 ccttgaacaa aagatgaaaa ttcaggccat tgtccctcaa ggtcttttta ataaccgtct
     1621 cattaaactt attaatatag agaattccta tctcacttcg aagcttcgcc acatcctcga
     1681 taatatcgta ggtctgggtc tctctcatgt gaaactcata tttatctcca ccatattttt
     1741 ttaaaagctc aacaaatgcc tccacaacaa aagaataatg ctgggcagag acacaaaatc
     1801 tctgcttgat gtggctgacg cccgtatact tctcctcaat caaattagcc tgatctaaga
     1861 cctgtctggc ataacctaaa aactcatctc cctcaggagt gatttctatt cccttgttgg
     1921 aacgagtaaa tatagtgata ttgtattcat tttcaagttc tctaattgca gatgtcaaac
     1981 taggctgagc tataaaaagt ttttttgccg cctcagtaat attgcctgtt tctgcaactt
     2041 caaccacgta tctaagctgt tttagtgtca tgtcttctcc ttatattaaa actactcaat
     2101 agattcttta gctattatag cttttattat aggtaaaatc tatcaatcag tcataatttt
     2161 ttatatttta cctatctact cactaggcat ataatcctca tattgaacat attgtaaaac
     2221 attggtattg cgcagaaccg ttaaaactaa acaaaaagga gaataaaatg agcaaaatac
     2281 agacacctta cagatatgat tttgtaggaa gtttccttag accacaggct cttaaggatg
     2341 caaaagcagc ttttcaggct ggcaaaatca caaaagaaga attcgataaa gttgtaaatg
     2401 aagaaatcac aaaggttgta gctaagcaga aaaagcttgg ttaccacgta atcacagacg
     2461 gagaattcag aagaacattt tggcaccttg atttcatgtg gggctttgaa ggagttgccc
     2521 acgaaaacac aggtggcggc gttagcttca acggggagct cgcattactt gatgacacat
     2581 atcttgtagg aaaaatcaaa gcaaaggctc atccatttgt tgagtatttc aagttcttaa
     2641 agcagtttga ggatgaaaat actgttgcaa aatatactat cccagcacct gctcagactt
     2701 tccagcagat gattgtccct gcaaactatg agactacaag aaaattctat cctacaaatg
     2761 aggaattaat ccaggatatc ggagttgcat accaggatgt tatcaagcag ttctacgagg
     2821 caggctgccg caatcttcag tttgatgatt gtacatgggg tgccatcgta ggagatgctg
     2881 caaagcagag atacgcaagc cttggtattg atcttgagga tgttaaaaag cagcttcttg
     2941 aagttaataa ccttgcactt gagaacaagc cagctgacat ggtaatcaca tcacacattt
     3001 gccgtggtaa ctaccactct acattcttta caagtggccc atacgactca gtagcagact
     3061 acgtatttgc caaggaaaat gtagatgctc tcttccttga gtacgatgat gctcgctctg
     3121 gtggatttgc tccgcttgct aaggtatcac ctgataaaaa ggtagttctt ggtctcatta
     3181 caacaaagac accacagctt gagaacaagg agacaatcat taagcgtatc aaggacgcat
     3241 caaaatatat tccactcgat agactttatc tcagccctca gtgcggattt gcctcatgcg
     3301 aaatcggtaa caaacttact gaagatgagc agtgggcaaa gcttaaacta gttaaagaaa
     3361 tcgcagaaga agtttggggt aaataatcca tacatcaaaa ggcagatgct tgatgcatct
     3421 gccttttgtt atagtatagc ttctgtactt ttacttcaaa tgtagctttt ctataatata
     3481 attaattacg cttcgttctc ccacaattat ctttgctgta caagccattc cattttttaa
     3541 gctcgcttcg gttccatctt ttccagataa catcatattt ttgcatgcta cttttacaat
     3601 atagtatgcc tcacctgttg tttggtctac cgtaatatcc tttgaaatag aacttacaat
     3661 accttcaaaa taaccatact ctctagaagg gaatgccgca atttcaaatt taacattttg
     3721 gccttcttct atttttccaa tatcactatt ttcaacatat atctgagcat aaaaatccga
     3781 ttcttccttg ggatatattt tacccagagt agtcgattca ttaagatatg ttccaacctt
     3841 tacatcctca gtcgaataga agtatccact agaagcagcc ctaatagtgc aattatcgtt
     3901 ttgtatgccg tagctagtaa gataattttc gcattcatct tttttctcat tgtatgttaa
     3961 ttcctcagtg taaatattac ctttctctgt taaaatagca atttttttta ctgattcagc
     4021 cgtacctgtt gtattagcat ttaactctaa ttgtgtttca ctaagatttg aatttaaact
     4081 caagagcgta ttattaatac tttctatctg ggattcaatt tcagaaatct gttgtgtttc
     4141 caaactagat aaagctttag taagctcatt ttccatattt tcaacactcg aatataatgc
     4201 gtttctttgt tcatataaag cttccttttc ttcatcatct tcagcttctg agatttgact
     4261 attgcatgta tctatctggt tattatactc agaaatcttg ttattatatt gaactctgtt
     4321 gtaattgtat gaagctaggt agctattaac catgctataa taatatgtct cttcgggttg
     4381 aaaggtattt tcacgattaa gaatacaatt tttaacctct gtaagcttct caaccttttc
     4441 agtatagacc gcaattgttt catttgtatt atctatactt ccttgatata ttgatatctt
     4501 gccttccaaa tcctcactac ttactgaaat attctccaat aacagttttc tcttattaac
     4561 aaactcatca tagtatgggt tatcctttag actatccggt attccggcac cagtatctaa
     4621 actttgctca tatgcatcta atatctctaa tctattttct gcatccgttt tctctttttg
     4681 ataattttga atcgtttctg agagagattc tatattcact acaaataaaa catccccctt
     4741 ttccacaaac tgtccgtcct ctacatttgc ctcttttaca caacctgtta ctgcgctact
     4801 aacctcaaat atagaatctg tccccttgaa tacaccattg ctctttacca cattatcaag
     4861 tttaaaattt actgtccaaa taacagatat tactagcatt agaaatatag tatatatcgt
     4921 atatactatt gcagggttag gctttgattc atatacctct acgctttctg atatttcccc
     4981 tatatctata aaaactggtt tcatgatacc tcaccttcct cttttagctc ttttatattt
     5041 tcactgatag tgatttcgcc taaagactgt tgccgtacca gctctgcata cttaccacct
     5101 agtcgctgca attcctcgtg atttccactc tcaattacct ttcctttttc cataacataa
     5161 ataacatcac agttcttaat tgtgctaagt ctatgtgcaa tgaatatagt tgttaaatct
     5221 ttagcaaatt tattgattgt ttcatctaat gctctctccg taacagcatc taaattactt
     5281 gttgcctcat ctaaaatcag gatatctggt ttctttagca tagctcttgc gattgccaat
     5341 ctctgtcgct gtcctcccga aagattagaa ccattttctt ctaacatagt ttcatatcta
     5401 agcggtaaat catttataaa gtcgtgagct tgagccattt ttgtagcctc aattacatca
     5461 tccatgttta catcatctaa tcccaagatc aagttttcat atattgttcc agaaaataaa
     5521 aacgtttctt gtggtatata tgcaatcttt tctcgcaatt tttctatctg aatatccttg
     5581 atattgtttt tattaatgag tatttctccc tcttctggtt gatacaagtt taataatagt
     5641 tttgatagag tagtttttcc tgaaccactt tcacccacaa atgcaactcg ctgaccttgt
     5701 ttgatattta aactgacatc atcaataact agtctacgag ttccatatct aaacttgata
     5761 tttttcattt ctatatctcc agcaattctt tttggctgaa gttttctaga ttcaccttcc
     5821 tttttctccg cttctaagtc taatatttca ctcaatctat cggctgcaac tattgcggtc
     5881 tgcatctgtg gctgaagatt tattagattc ttaactggat ctaagaaata tgctaataaa
     5941 gaattaaatg taatcagtcc accaattgtc atttcacctt tgattacact taatcctcca
     6001 acccagagga ttacaacccc acctactaac tcaattattg tctttaaaga agtctgaata
     6061 ttaccaaccc atgtcagatt aaaaatactt ttcagcaact ttacgaactt tttctctgtc
     6121 tcatgattta cttttcgctc tgcattatag gattttactg tctgaattcc atttaaagac
     6181 tcaaccatat atgaagtcag ctgagaatta tcttccatct gctttctatt aagcctctca
     6241 tacaaaccgt taaatgcaag cacaatcaca aaatacaaca caacaattat agccgtaatt
     6301 ccaaacaact tagcgttttg catataaaga ataatccctc cggctattgc catcaaagta
     6361 tcgatcataa tggtaagtgt agctcctgaa atagcgtcac gcacctttcc ggcatcatta
     6421 aaccgggaaa ttatttcgcc tactcttcgc gttccaaaga agttcatagg aagctctaaa
     6481 atatgctgat agtatcctaa caacatcgca atatccaact tttgactaag atataataat
     6541 agatgagtcc taaatgcatt taatacaact ctaaataagt taagtgcaat tactccaatt
     6601 gataaagtca ttaatgtttt cgataatgca tccggcaata ccgagtcaat catctcttga
     6661 aaataaaagg cccctaatat tccaagaatc gtatacacta gcgaagcaat aaatatgtgc
     6721 accaacaact ttttctgagg cacaagtaaa taaaaaaagc ggctaaacaa tccttttgtt
     6781 tcgtcgcctt ttacaaatgt ttcttttttg actaaaagaa ttaaaacacc gctccattgg
     6841 tatgttggtg gcttattatc ttcgtgtact tctccaaaaa actcttccgg gctaatcttt
     6901 atcaaacctt ttgctggatc tgctattgta acttgttttt ttgtaatttt atgtataacc
     6961 acataatgaa gtagtttccc atctactatc acatgcgcta tacaaggcaa aggtatttcc
     7021 gagaaaaaac cttcttgggt tccctttaca ccttttgcac taaacccaag ctcttcggct
     7081 gctttaatta ctccaaaagc atttgttcct tttttatctg taccagcaat ttctctaatt
     7141 tttgaaatgc tgagtttata accattttgt ttgcagattg tagcaaggca tgccgcccca
     7201 caatctgtta tatcgtgttg tttgacacag taatacttca tttctccctc gcaattatat
     7261 ttgtttgttc ttttccatta tcaaattttc atttaatgta aatgtgcgaa ctactccttt
     7321 ttcaaataca ttgtagaagt tgatatcata atatttgaac caacatcaca caaagcactt
     7381 gttagtcctg gttgcatttt gactagtttt ccataaatca taaaattctg aattatagcc
     7441 agaagtatag cgaaaaataa tagaaatgga ttgctacaca actgtaattg actatcatct
     7501 attgacactc gattattttc tttagtaaac aatacaacag atgtgaaccg acacattatt
     7561 acatcgaaaa tcattttcca atcccactga ttagactttg caaatgctag cgcaacacat
     7621 agaatcgtca ctttaaatat ctatttgttt ttcttccaat actttatcca tttgaaaatg
     7681 atgtaaactg cattcaaaaa catgcagata atctccacca aaaaaatata tcttattacc
     7741 atattatttc taaagggtaa taaaaataat gaaaaaaata gaattataca aagtactttt
     7801 gatcttgtaa aatcagattt atctaaaaaa ttcataaaat taacctcttg aaaaaatatc
     7861 caactcctac aatttcttta gaatctactc tctaagcttt ttatttattc gcaatacatc
     7921 ttcctgatag tatgaaggaa ctataactgt gcatttttct tcctcaaaaa taaaatcaat
     7981 tggagtagtc agccaatctt tatatttgtt tccaaattta aaactacatg tttcttttgt
     8041 ctcaacttta ttatttcttt tccatttttt gctagccact tcagaaagtt catttattag
     8101 acctcgccga tcttttaccg tgaatgttat tgaactcggt tttgctacta catagaaaaa
     8161 taaagtcata aaaaacacaa aaaacaaatc taataatact attactgaaa gaaataacgg
     8221 attaaccagc tttccaccat caatcattat taatgtaaaa aatattataa acaccactaa
     8281 tcatattatt ccaaaatgta ttaaataacg attgaacgta ttcacttgtc cttctaaaag
     8341 acccccaatt cttagctaag aattggggca tctatttatt tatttctcgt tttactaagc
     8401 aagagaaacc atatacctca aataatgcaa tacccccatg tatttcgcaa atttgtattc
     8461 tttagtttat ataaaataca tttgccatag gtagaccaaa taatcattat cctagtataa
     8521 tttataataa atattcccac atatgctatg atttaaacta ttttgtcttt attgcatcat
     8581 ttaacgatac gacaatcgtt atgtttcaaa tagttaatta gagtgcgcat tattatctta
     8641 cagtcttttt tctgtagttt atatcaacat ctaccataac catcgccgcg attatcgatc
     8701 caacacttcc caaattatta cttgccacta tattctctgt aattactata tataaggctc
     8761 caaataatag cagtattcta aagactgcaa tccatctgca aagtttatcc actaacgatg
     8821 attcaagtaa atttctatta actaatattt ttacagtagt aacaacagct actcctatgc
     8881 ataaaatgtt tcttacataa aaaataggct ccaaatcctt tattataacg gcattgtatg
     8941 ccgccgaaat acttactgca gtaaaatata ttattaataa tccaaaattt actttccact
     9001 gtaatttcat aatatcaact cctaatctga ctaatcatta agaccatcga ttatacaacc
     9061 tacaccaccc aaagctgtac aaacaccgcc tgcactaaaa gctactgttg cacattctcc
     9121 aacaccagca gctaccaata gtactccgcc tgcaactttt aggccattaa ctcctccatc
     9181 aattatctgt aattcattat ttgttaattc tctcatcaat gcatcctccc aaatttataa
     9241 attaccaaat tgatataaac cattccctac aagtgccact gctgcccaag gctgtcctgc
     9301 agccaatgcg ataggtgcag cagcaatcga aactgttcca agtgctgcat atccaaattc
     9361 agcccaatcg ccaccttcaa caagcatctc atccattgtt gaaagctgtg aaaaatcatt
     9421 cgataatact aaatccatgt tttacatcct ccgtaatttt ttattatttt tgaagaacgt
     9481 gattcgcgaa ttcacttcca aaattaattg tatatatgta aatcctcatt tcaataaaaa
     9541 tgtgacaaac cacactattt tgtgacgaac tgcactttct atagcaatta ttgctcacag
     9601 tcccctaata attgtcctcc tctgtatata atttgaattt aaaaatgaag gaaggcagat
     9661 tgcttatcga atctgccttt gttctatcta aattagtatt tcaatattga acataccatt
     9721 ttgagccttt atgtttagta ttccattata tttatttaca accgctttga tgttacttaa
     9781 cccatggcca tgatatgttt tgtcattctt agtagtttta atttctccat tatttatcat
     9841 tatttcatga ttagtaggat tatttgccat aatgcataaa gaattattat aaggataact
     9901 aacaagtctt atactatcgc cctttttaca tgcctcaatc gcattactaa gtatatttga
     9961 aaatataata cataagtcat aatcctgcac tttgtttttt cccaataact gtacctgcat
    10021 ctgcaagccg ataccatttt cctcacattt gtccttataa ctcctcaata ttccatctac
    10081 agcaacattt cctgagatgg ctgtattatt aattggtttt gcattatcta ataatgtttc
    10141 acaatattcc tttagctcat ctattctaga tttttcagcc atatgatgta tggcatttaa
    10201 atgagccgct aaatcatgtc gatatttatg atactttttt tcgctttcaa gtatggcatc
    10261 tacttgctct ttctgagcct ctagtgtcac tttttggtat agtagttttt tctcttcatt
    10321 ttgaattttt ctgttcaaaa aaatagaata tatcgttaac aaatatataa cgctacataa
    10381 tatagttgtg aagactatat aaatggttat aatatatgct ccattattcc cactttgaat
    10441 attagctact gcccccatcg aaatcgcaat aaaagccgta aaaatattta ttattatcag
    10501 ctcaacattt gtaaaattta aatcattcca aagaaaatct tttttctttt ttaaaaaata
    10561 cgctaatata attaatcccc aagtaggcga tacacacgca aaaattctat aatgcatttc
    10621 tagggcaaaa taatacgata ataatcccca aactgtattt aatgatatac ataacccaaa
    10681 gataggataa tataaaccta tattagttct tttttcacca ttaaatgccg gcagtagaaa
    10741 caatcccgct aataaagata tctttgttgg tccaaaatta taaataatcc ctatcaatat
    10801 agttgccaac tcgattaaac caaccacaat ccaataggtt actttcatct ttttgaactt
    10861 tatatctaat aactttacat atgcccagat aaattgccca agcaacagaa ttataaatat
    10921 tagttttgcc atcctactct ccactttatc ttttcaagca taaattcatt gtgggcattt
    10981 tttgtatcat taacatagtt cctccccagt ggaacacacg tattcccctc taattcgact
    11041 tctttataat gtatactcgt aatataattc atattcacta aaaaagactt atgacatctt
    11101 acaaaattag cgtctaatcg tttctcccaa ttggcaattg tgcctctttg gagtatacct
    11161 ccactacgat caactagcac aatcctaaca taatttcctt ccgcctctat gtattttatt
    11221 gagtcttctt taatctttgc gttcttgtct atatttatat atctagtatc tttaaatgcc
    11281 tcatacgcga tatttaccca atgaataatc tgattctcat ctattggttt ttccataaat
    11341 ccaataacat tttttccaaa tgcttctttc ataaatccaa tatgattagt aacaaaaact
    11401 atgtttgaaa cattcatttt cgtagttaat atatctttta cttcgatacc acttttccct
    11461 ggcatttcga tatctaaaaa taacaaacat atctgctcat taacattttt taataaatcc
    11521 tctccagatt tatattcttt aatttctgct tttattccta tactttcaaa atgcttaagt
    11581 actatatcaa taatagcttt tctgttttct ttatcatcgt cacatactgc cacaagaaaa
    11641 tttgattcca tttttccccc tttatataac ttgccctaat cataatacta tataataggc
    11701 ctcaaacaaa gaaaaagccc ggtaaatacc gagcttttta agattttcat taactaaata
    11761 accagttgtc accccacatg atatatcctc ctttgttgtg tctcatattt ttttgtgggt
    11821 acacaatacc ttattcgtct aatattttca agctttttgt gacgaacgac gctatttttg
    11881 tgataaacaa cgttttcctg ataaaaaaga agtgcaaaca gccaaaattt tgccctctta
    11941 gaattcagta taattttctc gtttatgtca tctcttttta atacattctc aaatacttat
    12001 gacattcctc atacttttcc aaatactatc gagaagaatt ccctgtagta attaaacaag
    12061 taatccgatt caaaataccc tttcaaataa tcaaaataat ctatagctct agaaatatgc
    12121 ccagtatcaa acaatgcaca ccccaacaaa taatctatat gcttcccttc gctattccct
    12181 tcactatgta acattccatc ggttgctata ctattattat tattattctt tctatacaca
    12241 a
//
