LOCUS       NZ_FOLK01000003        10977 bp    DNA     linear   CON 22-DEC-2024
DEFINITION  Streptococcus equinus strain Sb13, whole genome shotgun sequence.
ACCESSION   NZ_FOLK01000003
VERSION     NZ_FOLK01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus equinus (Streptococcus bovis)
  ORGANISM  Streptococcus equinus
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900112385.1-RS_2024_12_22
            Annotation Date                   :: 12/22/2024 07:11:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,845
            CDSs (total)                      :: 1,809
            Genes (coding)                    :: 1,793
            CDSs (with protein)               :: 1,793
            Genes (RNA)                       :: 36
            rRNAs                             :: 3, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 3 of 16
            Pseudo Genes (incomplete)         :: 10 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:39:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10977
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOLK01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10977
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10977
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10977
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3829:5977](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3830..5977
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(106..837)
                     /locus_tag="BM262_RS06070"
                     /old_locus_tag="SAMN05216408_1259"
     CDS             complement(106..837)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295306.1"
                     /locus_tag="BM262_RS06070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216408_1259"
                     /product="response regulator transcription factor"
                     /protein_id="WP_074869385.1"
                     /transl_table=11
                     /translation="MLDIFILEDNITQQFRIERQIETIMERNHWEYQRLEAFAASKDII
                     KKADGQGNHQVFFLDLEIKGDEKQGLDVARSIREKDPTAIIVFVTTHSEFMLLTYQSLV
                     GAIDFIDKNMNDEAFGGRIELCLKEALKYQNGSFGENSYLFETAKARIRVAYDDILYFE
                     TSPTVHRVILHTKTGQTEFYGTIAEVAKSDKRLLKCHRSFVINVNNVTEFDKSTRTAYF
                     ENGSYCQVSRDKVKKLMAEMR"
     misc_feature    complement(1153..1162)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     misc_feature    complement(1307..1322)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: strong, score: 26.79"
                     /tool="antismash"
     gene            complement(1603..2292)
                     /locus_tag="BM262_RS06080"
                     /old_locus_tag="SAMN05216408_1261"
     CDS             complement(1603..2292)
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014566.5"
                     /locus_tag="BM262_RS06080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216408_1261"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_074869387.1"
                     /sec_met_domain="Abi (E-value: 2.8e-19, bitscore: 63.1,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKQIYQILIERLKIVFVCIAFFLLHVLIQVPAASAIPDLGKNTA
                     IIIFIRIILILAVIPIAILLRNYLNYFSKGTEIFSSIQDVTIRKKDIQVIIEIAVVDLI
                     TTAGYIAINGMPEDKTLCKILLDNPLILVLSILTMGFTQPFLEEFLYRGILLGGLRHYS
                     KVLAVIVSSFVFSYIHVYDYHEVINLQLIGGLIYGLTFLKTNKLYPSIILHSMHNLIIL
                     VLVLWHG"
     gene            complement(2357..2509)
                     /locus_tag="BM262_RS09320"
                     /old_locus_tag="SAMN05216408_1262"
     CDS             complement(2357..2509)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM262_RS09320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05216408_1262"
                     /product="hypothetical protein"
                     /protein_id="WP_159428313.1"
                     /transl_table=11
                     /translation="MKNIEKFQVLTSEELEYTSGGKAFSSFAYESVRVAYSFLDGLNGR
                     KYRGN"
     gene            complement(2523..2663)
                     /locus_tag="BM262_RS09200"
                     /old_locus_tag="SAMN05216408_1263"
     CDS             complement(2523..2663)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM262_RS09200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05216408_1263"
                     /product="bacteriocin class II family protein"
                     /protein_id="WP_159428324.1"
                     /transl_table=11
                     /translation="MKKMEQFISLDEDSLSQINGGDGFTRAAYLLGRQIREVKKAFFNK
                     F"
     gene            complement(2686..3294)
                     /locus_tag="BM262_RS06085"
                     /old_locus_tag="SAMN05216408_1264"
     CDS             complement(2686..3294)
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014566.5"
                     /locus_tag="BM262_RS06085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216408_1264"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_074869390.1"
                     /sec_met_domain="Abi (E-value: 2.9e-16, bitscore: 53.5,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVGNLSTNKTKYHNIFLVINLIAINTLVSFLAHFDFNIFFSIVSI
                     IILFLWFFVIVNPYFTKLNLPDRSDWIKVLLLVVINEIIFYVTVSQIPNIQLSRISDFK
                     LNVASMLMAIIIGPITEEIFFRGIIANCFKSEIMNIIVSCLLFAILHGSVNLITVVLYF
                     VFALSSFYLYRKKGHLIDSILFHIFFNLSSIFFSIVNQI"
     gene            complement(3269..3433)
                     /locus_tag="BM262_RS09325"
                     /old_locus_tag="SAMN05216408_1265"
     CDS             complement(3269..3433)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF038034.1"
                     /locus_tag="BM262_RS09325"
                     /note="Among the bacteriocins in this family, lactococcin
                     G-beta was reported to be the beta chain of a two-chain
                     bacteriocin, while enterocin 1071B was reported to act on
                     its own; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216408_1265"
                     /product="lactococcin G-beta/enterocin 1071B family
                     bacteriocin"
                     /protein_id="WP_159428325.1"
                     /transl_table=11
                     /translation="MRKDKLMKENREYVILTDEQLSGISGGKIGWISLVDPVYNLVSGL
                     YDGWKSKHK"
     gene            complement(3436..3591)
                     /locus_tag="BM262_RS09330"
                     /old_locus_tag="SAMN05216408_1266"
     CDS             complement(3436..3591)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM262_RS09330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05216408_1266"
                     /product="bacteriocin class II family protein"
                     /protein_id="WP_159428317.1"
                     /transl_table=11
                     /translation="MKNLEQFDVMTSAELSNVEGGKSFFYDFGYWYGSHASLSNLANNL
                     GRMLRS"
     gene            3830..5977
                     /locus_tag="BM262_RS06090"
                     /old_locus_tag="SAMN05216408_1267"
     CDS             3830..5977
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364968.1"
                     /locus_tag="BM262_RS06090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216408_1267"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_074869392.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.1e-44, bitscore:
                     144.3, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 0.0, bitscore: 1108.0,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRYKYVSQIDMRDCGVAALASVAKHYGSHFSLAHLRELAKTNKE
                     GTTALGIVEAAKKIGFETRAVKADMVLFEMDDIPYPCIVHVNKQGKLQHYYVIYKAKKD
                     YLIIGDPDPSVGVTKMTKADFAKEWTGVTIFLAPAASYKPHKDKKNGLMSFLPIIFKQK
                     ALLTYIILASLLVTLINIVGSYYLQGILDDYIPNQLQSTLGIVSIGLVVTYIMQQIMSF
                     SQQYLLVVLSQRLTIDVILSYIRHIFELPMSFFATRRTGEVISRFTDANSIIDALASTI
                     LSLFLDVSILFIVGSVLILQNAKLFLITLIALPVYAVIIFAFMKPFERMNHDVMQANSM
                     VSSAIIEDINGIETIKSLTSEETRYRNIDSEFVEYLDKSFTLNKYEAMQASLKQGAQLI
                     LNVAILWYGSRLVMTGKISVGQLITYNTLLSYFTKPMENIINLQTKLQSAKVANKRLNE
                     VYLVSSEFDNKQVIDNQEFLKGDLVFDDVSYKYGFGHNTLSHIKLHIKEGEKVSLVGVS
                     GSGKTTLAKMMVNFYSPNQGQITLGGYDYKTIDKKVLRQHINYLPQQSYVFSGSILDNL
                     TLGASDAITQEDIIKACEIAEIRADIEAMPMAYHTELSDGAGLSGGQKQRLAIARALLT
                     KSPVLILDEATSGLDVLTEKRVIHNLLALKDKTIIFVAHRLSIAKQTDRVIVLDKGQII
                     EQGHHEQLMQNPGFYAQLFQE"
     gene            5987..7369
                     /locus_tag="BM262_RS06095"
                     /old_locus_tag="SAMN05216408_1268"
     CDS             5987..7369
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009570339.1"
                     /locus_tag="BM262_RS06095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216408_1268"
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_074869395.1"
                     /transl_table=11
                     /translation="MNPKLFQSAEFYHRRYHNFATLLVLPMTAFVLFLLLFSLIGKKEI
                     TVTSIGSLRPTKIIDVVQSTSNNTVLVNHLSENKTVKKGDLLIQYSDKLEESQIKAIQT
                     QIERDERQQNALNTLKDSLNQNKNLFSGDDEFGCAATIDRFLSQSKTITAQVSQANDSV
                     AKQEAGVNRANAAVNKQISELQTQASQYLEVKDAIQNNQTSVSGGNPYTATLNSYLSQL
                     QALPSDAATSNKESLKNQFIADLQGQIDSINSSISSLQTQAASNYSTGSYDTSAASQIE
                     SLRQQELTQAETQLAQVTQEKESLQAQLDQTSLSKSDTVLKAKENGILHVSDEFEGKTL
                     LPQGSQIAEIYPDIVKTKKVAISYYVDSTHVSQLKKGQCVRLTLEKISNHAIIVSGKIS
                     KIASSATQTKSGNFFKVTATAEVTEKDSSKLKYGLQGKTISIIGKKSFFNYYKDKLLKD
                     FQ"
     gene            complement(7395..7574)
                     /locus_tag="BM262_RS06100"
                     /old_locus_tag="SAMN05216408_1269"
     CDS             complement(7395..7574)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BM262_RS06100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05216408_1269"
                     /product="hypothetical protein"
                     /protein_id="WP_074869398.1"
                     /transl_table=11
                     /translation="MKKSQQKFSWLVAVSAVSVIWIKNFILESNDLSEKLVYIGIYLLY
                     LVLVFVKVFKPRKK"
     gene            complement(7914..8588)
                     /locus_tag="BM262_RS06105"
                     /old_locus_tag="SAMN05216408_1270"
     CDS             complement(7914..8588)
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962518.1"
                     /locus_tag="BM262_RS06105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216408_1270"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_074869400.1"
                     /sec_met_domain="Abi (E-value: 3.8e-17, bitscore: 56.3,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEKVKNLLAPKLLAIFFIYYHLSGLKFVVFTILERFDLPLTEYH
                     YPLLNIFTILFIIVLVIYLRRRRPEIFQIRPLKPLDYLVTLLAFGVIYAYFSNLLFVGG
                     DIRNDLLFFRDSPLAFLVALQPFIIGPILEELVYRGVLMTSFFEKSKFYLDCLLSAVMF
                     SVAHLSSYGLSLEVFKNYAFLGFILAILFRKTRSIYPSILIHMAWNIYLSWHLITFIFF
                     ER"
     gene            complement(8686..8844)
                     /locus_tag="BM262_RS09205"
                     /old_locus_tag="SAMN05216408_1271"
     CDS             complement(8686..8844)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515596.1"
                     /locus_tag="BM262_RS09205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216408_1271"
                     /product="bacteriocin class II family protein"
                     /protein_id="WP_081351173.1"
                     /transl_table=11
                     /translation="MNTKTIEQFNTMTTEELACVEGGNQVFAGGGGVLKPDYPWWRIDP
                     LIDFRLL"
     gene            complement(9214..10086)
                     /gene="rsmA"
                     /locus_tag="BM262_RS06115"
                     /old_locus_tag="SAMN05216408_1272"
     CDS             complement(9214..10086)
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535644.1"
                     /locus_tag="BM262_RS06115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216408_1272"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_074564489.1"
                     /transl_table=11
                     /translation="MRIADYSVTRAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     KNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLRDFDNVKVINEDILKS
                     DLQSRIKEFANPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMMQKEVADRISAE
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMTRREQPLVQVKDEDFF
                     FRIAKISFVHRRKTLWNNLTSHFGKAEETKAKLEKALEMAEIKPSIRGEALSIADFAKL
                     ADALKEVGL"
     gene            complement(10175..10699)
                     /locus_tag="BM262_RS06120"
                     /old_locus_tag="SAMN05216408_1273"
     CDS             complement(10175..10699)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852270.1"
                     /locus_tag="BM262_RS06120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216408_1273"
                     /product="DUF1697 domain-containing protein"
                     /protein_id="WP_074869403.1"
                     /transl_table=11
                     /translation="MKKVALLRGVTPVGQNRIPKMTYLVEILTEAGLENVKTYIQSGNI
                     VFETDLADDAICTLIHDTIKEKIGADLSVVLKEQPQLTTAIIENPFDESYDASRVHLVF
                     TNDQFSQYKLADLLETDFGDEILRAGSECLYLYLPREAKKKRLNTNYLEKQLHITATMR
                     KLRSVSRLSQM"
     misc_feature    10655..10670
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.86"
                     /tool="antismash"
ORIGIN
        1 cgtaagttta gtttttctcc tcttacattc gcaaaaattg tcatgataac aggataataa
       61 gtaagttgtg aagcataaaa aattaaaatt gcttttacca tcccatcacc tcatttctgc
      121 catcagtttt ttgactttgt ctcttgagac ttggcagtaa gagccgtttt cgaaataagc
      181 tgttcgggtg cttttatcaa attcagtgac attattaaca ttaatcacga atgagcggtg
      241 acatttcaac aaacgtttat ctgatttggc tacctcagca attgtgccat aaaactctgt
      301 ttgacctgtt ttagtgtgta aaatcacacg gtgcacggtt ggcgatgttt caaaatataa
      361 aatatcatca taagccacac ggattcttgc ttttgctgtt tcaaacaggt aagaattctc
      421 accaaaacta ccattttgat atttcaatgc ctctttcaag cacaattcaa tgcgccctcc
      481 aaaggcttca tcattcatat ttttatcaat aaaatcaatc gcaccaacta gtgattgata
      541 agttagaagc ataaactcag aatgggttgt cacaaagaca ataatggctg tcggatcttt
      601 ttcacgaata gatctagcaa catctaatcc ttgcttttca tcccctttga tttctaaatc
      661 aagaaagaaa acctggtgat ttccctgccc atctgccttt tttataatat ccttagatgc
      721 cgcaaaagcc tctaaacgct gatactccca gtggttcctt tccataatcg tctcaatctg
      781 acgttcgatt cgaaattgtt gcgtgatatt atcctcaagg ataaaaatat ctaacataaa
      841 cctctcccta cctaaactat tcttttgatt atcttaaaga ctaacatttt ctattataaa
      901 ggaaatttta ggaaaaaaac acctatgtcg taaacagtta atttttcgtc gtaaacggca
      961 tttagaacta aaaaagctga cagaataaac tatcagcttc acctatcata atagtaatac
     1021 ctaataaatt acttcaatta aattgttatc agttaatatc cctattttca taatagatca
     1081 acatcaaaac aagtaaacaa actattgaca atggagtcgt agtaccttct ttttgagtta
     1141 aagaataaaa aatcaaaaaa caaacataag tagacgtttt tctactcttc ttgatactaa
     1201 agccatcaat agcaattatt gccaaaataa cacaatataa aataacaaaa atagaataac
     1261 acataaaaac taatttactt tattaatatc atacaatata aacattgtga aaaaaacgag
     1321 aactcctttt actaacatac catccatcga taatactgcc ttcgctccca atgctaaaaa
     1381 cagtatattt tttagaacag ttctccaatt tgtcatgttt tctttccaat tttacttttt
     1441 attttgtcat tacaactgac aattcttagt ttagcataaa ttttaattga taaacgttaa
     1501 tgtcataaac ggttaaaatt cagtcgcgaa cggtaaataa gataaatcaa tctggcattt
     1561 tgaaatagct tgatgactta caccaactta ctttgaaacc acttaaccat gccataacac
     1621 taatactaaa ataattaagt tatgcatcga gtgtaagata atggaagggt ataatttatt
     1681 cgtttttaaa aaagtaagtc cataaattaa tccaccaatt aattgaagat tgatcacttc
     1741 atggtaatca tatacatgaa tataactaaa aacaaagcta ctaactatta cagccaacac
     1801 cttagaataa tgcctcagcc ctcctaaaag aattcctcta tacaaaaatt cttctaaaaa
     1861 tggttgtgta aatcccatag ttaaaatact taaaactaaa atcaatggat tatctaataa
     1921 aattttacat aatgttttat cttcaggcat gccgttaata gcaatatatc cagctgtggt
     1981 aatcaaatca accaccgcta tctcaataat tacttgaata tctttttttc taattgtcac
     2041 atcctgaata gaggaaaaaa tttcagtccc ctttgaaaaa taattaagat agtttcgaag
     2101 taatattgcg atggggataa ccgccaatat caatattatc ctaataaaaa ttattatggc
     2161 agtattttta ccaagatctg gtatagctga tgctgctggt acttgaatta atacatgtaa
     2221 tagaaaaaag gcaatacaaa caaatactat ttttaacctt tcaattaata tttgatatat
     2281 ttgtttaatc atagtaatct ccttaacaaa ctaaggagta ctacttgaag tactcctcaa
     2341 tttattattc tatttcctaa ttccctctat actttctccc atttaaccca tcaaggaaag
     2401 aatatgccac tcgaacactt tcataagcaa aacttgaaaa agccttccca cccgatgtat
     2461 attcgagttc ctcactagtt aaaacttgaa atttttcaat atttttcatc tttatctcct
     2521 acttaaaatt tattaaaaaa agcttttttc acttcacgaa tttgtcttcc taacaaatat
     2581 gcagccctag taaatccatc gcctccatta atttgtgata aagaatcttc atcaagagag
     2641 ataaattgtt ccattttttt catactcata aaatctccta aacctttata tttgattaac
     2701 tatagaaaag aatatactac tcaaattaaa aaatatatga aataaaattg agtctattaa
     2761 atgtcctttt ttcctatata aataaaatga acttaacgca aaaacaaaat ataaaactac
     2821 agttattaga ttaacactac catgtaaaat ggcaaataat aaacaactta caataatatt
     2881 cattatttca cttttaaagc agttagctat aatacctcta aaaaatatct cttctgttat
     2941 aggaccaata ataatagcca ttagcataga agccacattt aatttgaagt cactaattct
     3001 tgataattga atatttggaa tttgactaac tgtcacataa aaaataattt cattaataac
     3061 gactaataat aaaactttta tccaatcgct tctgtcaggc aaatttaatt ttgtaaagta
     3121 cggatttact attacaaaaa accataaaaa taaaattatt atagaaacaa tactaaaaaa
     3181 gatattaaaa tcgaaatgcg ccaagaaact aacaagtgtg ttaatagcaa taagattaat
     3241 aactaaaaat atattatgat atttagtttt atttgtgctt agatttccaa ccatcgtaca
     3301 aaccagatac aagattatac actgggtcta ccaatgaaat ccaccctatt ttcccaccag
     3361 aaatacctga caactgttcg tcagttaaaa ttacatattc acgattctct ttcatcaatt
     3421 tatcctttct cacaattaac ttcttaacat acgtccaagg ttattagcta gattagaaag
     3481 gctggcatga ctaccatacc aatatccaaa atcataaaag aaacttttcc caccttcaac
     3541 gtttgaaagt tctgctgatg tcattacatc aaattgttcc aaatttttca tgttttcttc
     3601 tccattttcc tttttatttt gtcgttgcaa ctgacaattt tagtttagca tagaatttta
     3661 tttgtgaatg taaatgtcgt aaatggttaa aattcaatcg cgaacggtaa aatcgatgta
     3721 agcaaatgac cttttacgac gaaaaaacga ccgtttatga cttggggtgt tacaagctag
     3781 ctttttgtgg cataatattt tttgactctg ttttagaagg aatggtttta tgaggagata
     3841 caaatacgtt agtcagattg atatgcgaga ttgtggggta gctgctttgg cgtcagttgc
     3901 taaacactac ggctcacact tttcgcttgc tcacttgagg gaactggcta agacgaataa
     3961 agaaggaaca actgctcttg gaatcgtcga ggcagccaaa aagattggct ttgaaacacg
     4021 cgctgtaaaa gctgatatgg tactttttga gatggatgat atcccgtatc cttgcattgt
     4081 ccatgtgaat aagcaaggaa aactacaaca ctattatgtc atctataagg ctaaaaaaga
     4141 ttatctaatc atcggtgatc ctgacccaag tgtgggagtt acaaaaatga ctaaagcaga
     4201 ttttgccaaa gaatggacag gggttacgat ctttctagct cctgcagcta gctataaacc
     4261 acataaggat aagaaaaatg gcttgatgag tttcttaccg attattttta agcaaaaagc
     4321 actactaaca tatataatcc ttgctagcct acttgttacc ttgattaata ttgttggttc
     4381 ttattatcta caaggaatct tagatgatta tattcctaac caactgcaat cgacccttgg
     4441 aattgtctca atcggcttgg tcgtgactta catcatgcaa cagattatga gcttctcaca
     4501 acagtatttg ttagttgtgc tcagtcaacg tttgacgatt gatgtcattt tatcttatat
     4561 tcgtcatatt tttgaattgc ctatgtcctt ttttgcgaca aggcgaactg gggaagtaat
     4621 ttcccgcttt accgacgcca attctattat cgacgcgctt gcttcaacaa tcttatcgct
     4681 ttttctcgat gtgagtatcc ttttcatcgt tggcagtgtt ttaattttgc aaaatgcgaa
     4741 actcttcttg ataaccttaa ttgctttgcc tgtttatgcc gtcattatct ttgcttttat
     4801 gaagcctttt gaacgaatga atcatgacgt catgcaagct aattctatgg tcagctctgc
     4861 cattattgaa gatatcaacg ggattgaaac cattaaatca ctaactagcg aagagacacg
     4921 ttatcgcaat atcgatagcg aattcgtgga atacttggat aaaagtttca cacttaataa
     4981 atacgaagca atgcaagcct cgcttaaaca aggggcgcag ctgattttaa atgtggctat
     5041 tctctggtac ggctcacgtc tcgttatgac agggaaaatt tctgttgggc aattgattac
     5101 ctataacacc cttttatctt acttcacaaa accaatggaa aacattatta acttgcaaac
     5161 taagttacag tcagcgaaag tggctaacaa gcgacttaac gaagtttact tggtatcttc
     5221 tgaatttgat aacaagcaag tgattgataa tcaagagttt ctcaaaggtg accttgtctt
     5281 tgatgatgtt tcttataaat atggctttgg acacaatact cttagtcaca ttaaacttca
     5341 tatcaaggaa ggtgaaaagg ttagtctcgt cggcgtcagt ggttctggga aaacaacttt
     5401 agctaaaatg atggtgaact tttatagtcc aaatcaaggt caaatcacct tgggaggcta
     5461 tgactataaa actattgata aaaaagttct tcgccaacac atcaactacc ttccacaaca
     5521 atcttatgtc ttttctggaa gtatccttga taatctcact ttaggtgctt ctgatgcgat
     5581 tactcaagaa gacattatca aagcctgtga aatcgctgag attcgtgctg acattgaagc
     5641 tatgccaatg gcttaccata ctgagttatc agatggggct ggtttatctg gcggtcaaaa
     5701 acagcgatta gctattgcca gagcacttct gactaagtca cccgtcttaa tcctagatga
     5761 agcaacaagt ggtcttgatg ttctgaccga aaaacgtgtt atccacaatt tgttagcttt
     5821 aaaagacaaa accattatct ttgttgctca ccgtttaagc attgccaagc aaactgaccg
     5881 tgtcatcgtc ctagataaag gacaaattat cgaacaaggt catcacgagc aactcatgca
     5941 aaatcctggc ttttacgctc agctatttca agaataagga ggaatcatga accctaaatt
     6001 atttcaatcg gctgaattct accacagacg ttaccataat tttgcgacac tcttggttct
     6061 tccaatgaca gctttcgtgc tttttttatt gctattttct ctcattggaa aaaaagaaat
     6121 cactgtcact tctattggta gtcttcgtcc tacaaaaatt atcgatgtcg tgcaatctac
     6181 tagcaataac accgttcttg ttaatcactt gagcgaaaac aaaacggtta aaaaaggaga
     6241 tttgcttatc cagtactcgg ataaattaga agaaagtcaa attaaagcta ttcagaccca
     6301 aatcgagcga gatgagcgtc agcaaaatgc cctaaacaca ctaaaagaca gcctcaatca
     6361 gaataaaaat cttttttcag gtgatgatga atttggctgt gctgcaacta tcgatcgttt
     6421 tcttagccaa agtaaaacca ttacggctca agtttctcag gctaatgatt cggttgccaa
     6481 acaagaagca ggggttaatc gagctaacgc tgcggttaac aaacaaatca gtgaacttca
     6541 aacacaagct agccaatacc tagaagtcaa agatgccatt caaaacaatc aaacaagtgt
     6601 ttctggtggt aatccgtaca cagccactct gaacagctat ttgagtcagc tccaagctct
     6661 tcctagcgat gctgctacta gcaacaaaga aagccttaaa aatcagttta tagctgacct
     6721 tcaagggcaa attgatagca ttaattcttc catttcaagc ctacaaaccc aagctgcttc
     6781 aaattactca actggcagtt acgataccag tgcagctagc caaattgaaa gtctacgcca
     6841 gcaagaactg acccaagctg aaacacagct agctcaagta actcaggaaa aagaaagctt
     6901 acaagcgcag cttgatcaaa caagcctgtc aaaatctgat actgtcctaa aagctaaaga
     6961 aaatgggatt ttacatgttt ctgatgaatt tgaagggaaa actcttcttc ctcaaggaag
     7021 ccaaattgca gaaatttatc ctgatattgt taaaaccaaa aaagttgcca tttcatacta
     7081 cgttgattca actcatgtta gccaactgaa aaaaggacag tgtgtgcgtc taactcttga
     7141 aaagattagt aatcacgcca tcattgttag cggaaaaatt agtaaaattg ccagctctgc
     7201 tacccaaacc aaaagtggaa atttctttaa agtcacagca actgcagagg ttactgaaaa
     7261 agacagctct aagttaaaat acggcttaca aggcaaaacc atcagtatta tcggaaaaaa
     7321 atccttcttt aactactaca aagacaaact cttaaaagat tttcaataag aataaccagc
     7381 caaattggct ggttttattt ttttcttggt ttgaacactt taacaaaaac taagactaaa
     7441 taaagaagat aaattcctat atagacaagt ttttccgata aatcgttaga ttctagtata
     7501 aaatttttta tccaaatcac tgaaacagca cttactgcta ctaaccagct aaatttttgc
     7561 tgcgactttt tcataaaatc ctcccattct ccttaatttt cttttttaag tgtaaaaaga
     7621 aaagggaagt aagcatccta acttactttc ctctaatatc atcttgaaaa cgatgaattt
     7681 tcaaagatag gtttctccca taatatctcg caagtgccta agattccctt tttacattgc
     7741 aacaacactt cttagtaaca ctgcgactaa agtccttatg tcatttaaat ctcctggaaa
     7801 tgacgtgtca tgtgactgac atatttaatt gtagcagaaa ttttgcaaaa taaccgtcta
     7861 tgtcgtaaac ggtcgttttt tcgtcgtaaa cggtaatttt ttcatgattt ttactacctt
     7921 tcaaaaaaaa taaatgtaat cagatgccag ctaagataaa tattccatgc catgtgaata
     7981 agaattgatg gatagattga ccgtgttttt ctaaaaagaa tcgctaaaat gaaaccaaga
     8041 aaagcatagt ttttaaatac ctctaaactc aaaccataag aagatagatg agctactgag
     8101 aacatcacag cagatagtaa gcaatctagg taaaattttg atttctcaaa aaagctagtc
     8161 atcaaaacac cacgataaac taactcttct aggattggtc cgataataaa gggctgtaat
     8221 gctaccaaaa acgccaatgg ggaatcacga aaaaatagta aatcattcct tatatctcca
     8281 ccaacaaata aaagattact gaagtaagcg tatatcaccc caaaagcaag caaagttact
     8341 aaataatcta aaggttttag tggtcttatt tgaaatattt ctggtcttct tcgtcttagg
     8401 tatatcacca gtacaataat aaataggatg gtaaaaatat taagaagagg gtaatgatat
     8461 tccgttaatg gtaaatcaaa tcgctctaaa atagtaaaca ctacaaactt taatccagat
     8521 agatgataat agataaaaaa tattgccaac agttttggag caagtaagtt ctttactttt
     8581 tctatcataa atactccttt tctaaaaaag aatgaagtca aataagatag cttaacttct
     8641 tttatcaatg ctcatttttc cagattcctc tagacaacta ttgtgtcaaa gtagtcgaaa
     8701 gtcgatgaga ggatctatcc tccaccaagg gtaatctggt tttaacactc cccctcctcc
     8761 agcaaagact tgatttccgc cttcaacaca cgcaagctct tcagttgtca ttgtgttaaa
     8821 ttgttcaatc gtttttgtgt tcattttaat gtctcctaaa ttatttttgt catcgcgatt
     8881 gacaagtttt attttatcag aattttcgca caacaaccga ctatgtcgta aacagtcgtt
     8941 ttttcatcgt aaacggtaaa cttttcaata atccttaccg tttgcgactg aatattaacc
     9001 gtttatgact tgagcgttca cagctataca ttttatggca taataaaagc gatattggta
     9061 actgaaattt ttaggagatt aacaatgagt ttgattatat tttataaatt agtgatgtca
     9121 cagccttggc atgacttttc aacagtagtg gcaagtttaa ctcatcatct attttaagca
     9181 taaaaaaagc aactctttcg agttgttttt ttattataaa ccgacttctt tcaatgcgtc
     9241 agctaatttt gcaaagtctg caattgataa agcttcacca cgaattgatg gtttgatttc
     9301 tgccatttca agagcttttt caagtttagc tttagtttct tctgctttcc cgaagtgact
     9361 tgtcaagttg ttccaaaggg ttttacgacg gtgaacaaaa ctgattttag caatgcggaa
     9421 gaagaaatct tcatctttaa cttgaacaag tggttgttca cgacgtgtca tttttaagat
     9481 ggctgaatca acgttaggcg ctggcacaaa gactgtacgt ggcacgatga aagcaacttt
     9541 tgctgtcatg tagtattgca ccgcaattga aagtgaaccg taagctttgg tatttggttc
     9601 tgctgagata cggtccgcca cttctttttg catcatcacg acaaattctg caaatggaat
     9661 ttttgattca atcaagtgca tcaagattgg tgttgtgatg tagtaaggaa ggtttgctac
     9721 gaccttgatt ggaaggtctg ggttagcaaa ttctttgata cggctttgaa ggtctgattt
     9781 caaaatatct tcattgatga ctttgacatt gtcaaaatcg cgaagcgtat cagccaaaat
     9841 cggaatcaat cggtcatcaa tttcaaacgc cataacttca gcagcatttt cagccaaaaa
     9901 ttctgtcaaa gcaccgatac caggaccaat ttcgataacg ttgacattct tatcaatctc
     9961 agcagtatcc acaatttttt gtagaatgtt agtatctgtt aaaaagttct gtccaaatga
    10021 ttttttaaat gtaaatccat gacgctcaag gacagcacgc gtcacactgt aatcagcaat
    10081 tctcataatt tatttaacta ccttatccac aagttatccc cagaataagg ttttgtcatt
    10141 cctttcgttt ctgtcaatgt aagttactat tgtatcacat ctggcttaaa cgtgaaacac
    10201 ttctgagctt tcgcatggtt gctgtgatat gtaactgttt ttcaaggtaa ttggtattca
    10261 gtcgcttttt ctttgcttct cttggtaaat agagatagag gcattcactt cctgctcgca
    10321 agatctcatc accaaagtca gtttctaaca ggtcagctaa cttatactgc gaaaactggt
    10381 catttgtaaa aacaaggtga acacgtgaag cgtcatagct ttcatcaaag gggttttcaa
    10441 taattgccgt tgtaagctgc ggttgctctt taagaacaac agataaatca gctccgattt
    10501 tttccttaat cgtatcatga atcaaggtgc aaatagcatc atcagctaaa tccgtctcaa
    10561 aaacaatatt gccactttga atataagtct ttacgttttc aaggcctgct tctgtcaaaa
    10621 tctcaacaag ataagtcatt ttaggaatcc gattttgacc aactggtgtg acacctcgca
    10681 agagtgcgac ttttttcatt cttcgtattt ctccattgct tcttcgactt cagccagagt
    10741 gatgccaaag agttctaagc gtttaaggag ttgtttaccg ttgctgtagc caatacggag
    10801 ttcttctccg aggtattcac ggcgtttgcg gctatcgcca cccattaaca gccccatgcg
    10861 catcaaatca gcttttgtga tgtcaaattg gttgtcatca tcaaagttcc ctaaaacacc
    10921 tttaagagcc ttttgtaaat cttcaaagct ggcatgttcg acaccaagtg aacgtcc
//
