GCF_900111885.1 - 24 region(s)

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Location: 125,957 - 150,393 nt. (total: 24,437 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene overview

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KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

BM199_RS22345: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Ligases: synthetase

BM199_RS22355: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.80	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0000673.3	0.54	terpene	pimara-8(14),15-diene	Aspergillus nidulans FGSC A4
BGC0000657.5	0.52	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus
BGC0000678.5	0.38	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000665.5	0.31	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001156.5	0.31	terpene	platencin	Streptomyces platensis
BGC0000644.3	0.31	terpene	carotenoid	Dietzia sp. CQ4
BGC0000653.5	0.31	terpene	pentalenolactone	Streptomyces arenae
BGC0000632.5	0.30	saccharide, terpene	brasilicardin A	Nocardia terpenica

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.80	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0000673.3	0.54	terpene	pimara-8(14),15-diene	Aspergillus nidulans FGSC A4
BGC0000657.5	0.52	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus
BGC0000678.5	0.38	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000665.5	0.31	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0001156.5	0.31	terpene	platencin	Streptomyces platensis
BGC0000644.3	0.31	terpene	carotenoid	Dietzia sp. CQ4
BGC0000653.5	0.31	terpene	pentalenolactone	Streptomyces arenae
BGC0000632.5	0.30	saccharide, terpene	brasilicardin A	Nocardia terpenica

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

BM199_RS22345: T1TS + T1TS

Domain 1 (1 - 329): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (338 - 704): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

BM199_RS22355: PT_FPPS_like

Domain 1 (68 - 362): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.58 out of 20.97

Confidence: medium

Additional weak hits (8):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.58 out of 28.14

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.76 out of 26.33

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.46 out of 33.14

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.06 out of 23.43

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.23 out of 27.78

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.37 out of 30.22

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A

NZ_FOKG01000011.1 - Region 1 - NRPS,NRPS-like,T1PKS

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Location: 139,710 - 226,073 nt. (total: 86,364 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

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TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

BM199_RS19155: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

BM199_RS19200: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BM199_RS19295: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Transferases: transferase

BM199_RS19260: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Hydrolases: dehalogenase esterase hydrolase

BM199_RS19115: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

BM199_RS19190: thioesterase

smcogs: SMCOG1004: thioesterase

BM199_RS19250: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

BM199_RS19255: putative esterase

smcogs: SMCOG1104: putative esterase

Lyases: cyclase hydratase

BM199_RS19080: Nitrilase/cyanide hydratase and apolipoprotein

smcogs: SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein

BM199_RS19220: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

Ligases: ligase synthase

BM199_RS19170: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BM199_RS19175: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BM199_RS19180: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BM199_RS19185: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BM199_RS19195: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BM199_RS19265: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BM199_RS19270: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	1.28	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001164.3	1.28	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	1.28	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001132.4	1.27	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001249.5	1.26	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001276.3	1.22	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	1.22	PKS	asperlactone	Aspergillus ochraceus
BGC0001128.4	1.22	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001275.3	1.21	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	1.19	PKS	(-)-Mellein	Parastagonospora nodorum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.76	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.75	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001249.5	0.75	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.72	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000342.4	0.72	NRPS	enniatin	Fusarium equiseti

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.85 out of 23.79

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.52 out of 29.72

Confidence: medium

Additional weak hits (8):

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.39 out of 29.51

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.64 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.50 out of 26.06

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.79 out of 30.22

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.53 out of 30.25

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.94 out of 19.52

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.52 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 139709 - 226073:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser) + (Trp) + (ccmal) + (X) + (X) + (Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 139709 - 197388:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X) + (ccmal) + (Trp) + (Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 158175 - 201706:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser) + (Trp) + (ccmal) + (X) + (X) + (Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 179485 - 226073:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BM199_RS19170: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (500..903): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAIDVTA
Nearest Stachelhaus code(s):: Ser DVWHVSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BM199_RS19175: Trp

Search NORINE for peptide: strict or relaxed

AMP-binding (459..856): Trp

nrpys: Trp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWATFDLTVTTGKAVLGGETNEYGPTEATVGCVA
Nearest Stachelhaus code(s):: Trp DLTKVGEVGK (85% 8Å match); Asn DLTKVGEVGK (71% 8Å match)
Stachelhaus code match:: 100% (strong)

BM199_RS19180: mal

PKS_AT (543..822): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 166.8
inactive: 97.4
Methoxymalonyl-CoA: 73.8
Methylmalonyl-CoA: 61.5
Propionyl-CoA: 45.1
Isobutyryl-CoA: 38.4
Acetyl-CoA: 37.2
Ethylmalonyl-CoA: 32.1
2-Methylbutyryl-CoA: 31.3
Benzoyl-CoA: 31.0
trans-1,2-CPDA: 25.2
fatty_acid: 25.0
CHC-CoA: 23.5
3-Methylbutyryl-CoA: 11.0

BM199_RS19185: X

AMP-binding (30..421): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGWAFDAFTMDAFIACGGELNVYGPTETTVVVLT
Nearest Stachelhaus code(s):: Leu DAMLAGAVAK (59% 8Å match); Val DAMFVGGVFK (56% 8Å match); ndmHex DAMVLGVVAK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

BM199_RS19195: X

AMP-binding (10..403): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VNQAFDAAIPDPHSLLGGEVNGYGATEVTFAAQY
Nearest Stachelhaus code(s):: Glu DAMHVGGTAK (56% 8Å match); Leu or Val DAIALGGCAK (41% 8Å match)
Stachelhaus code match:: 70% (weak)

BM199_RS19265: mmal

PKS_AT (634..927): mmal

ATSignature: Methylmalonyl-CoA, Malonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 66.7%; Malonyl-CoA: 66.7%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 117.4
Ethylmalonyl-CoA: 109.0
Methoxymalonyl-CoA: 103.8
Malonyl-CoA: 81.9
Isobutyryl-CoA: 73.8
Propionyl-CoA: 69.8
fatty_acid: 68.2
trans-1,2-CPDA: 67.0
CHC-CoA: 64.4
2-Methylbutyryl-CoA: 54.9
Acetyl-CoA: 43.9
3-Methylbutyryl-CoA: 34.4
Benzoyl-CoA: 32.7
inactive: 27.4

BM199_RS19270: NH2

CAL_domain (53..502): NH2

minowa: NH2

Prediction, score:

NH2: 112.0
AHBA: 16.2
fatty_acid: 15.4
shikimic_acid: 4.8
Acetyl-CoA: -0.3

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C

NZ_FOKG01000001.1 - Region 1 - NRPS

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Location: 21,103 - 73,850 nt. (total: 52,748 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BM199_RS00155: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

BM199_RS00275: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: methyltransferase

BM199_RS00210: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: esterase

BM199_RS33665: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase

BM199_RS00135: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BM199_RS00215: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BM199_RS00225: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.70	NRPS	enniatin	Fusarium equiseti
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.70	NRPS	enniatin	Fusarium equiseti
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.40 out of 19.26

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 22.42 out of 27.55

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.19 out of 19.26

Confidence: medium

Additional weak hits (14):

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.62 out of 32.93

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.98 out of 31.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 18.61 out of 28.14

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.11 out of 21.55

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.38 out of 29.65

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.58 out of 20.01

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.38 out of 20.97

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.75 out of 36.54

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.66 out of 20.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.21 out of 29.38

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.40 out of 30.25

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.22 out of 24.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.54 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 21102 - 73850:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NH2 - D-NMe-Cys) + (Thr)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BM199_RS00135: X

AMP-binding (11..411): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NNLAHIGALLSPFMLCGGAMEGFGMTELSPAALY
Nearest Stachelhaus code(s):: Val DALFLGGTFK (35% 8Å match); Ile DGLFIGIPVK (24% 8Å match)
Stachelhaus code match:: 60% (weak)

BM199_RS00215: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (481..882): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LSTHFDFSVWEGNQIFGGEVNMYGITEATVHVTQ
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

BM199_RS00225: NH2 - Cys

Search NORINE for peptide: strict or relaxed

CAL_domain (27..475): NH2

minowa: NH2

Prediction, score:

NH2: 209.3
Acetyl-CoA: 71.2
fatty_acid: 35.0
AHBA: 7.9
shikimic_acid: 7.2

AMP-binding (1136..1540): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TWVTFDLSVYDGDSFLSGDIGLGGATEAAIWSNF
Nearest Stachelhaus code(s):: Cys DLFDFSLVWK (79% 8Å match)
Stachelhaus code match:: 80% (moderate)

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
GnfM	Regulator of nitrogen-fixation genes

NZ_FOKG01000001.1 - Region 2 - lanthipeptide-class-iii

BGC0000553.3 (SSGG_RS09925):

Download region SVG

Location: 133,332 - 155,911 nt. (total: 22,580 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BM199_RS00700: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

BM199_RS00725: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Lyases: hydratase

BM199_RS00720: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000513.3	0.83	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000496.5	0.62	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000501.3	0.60	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000518.3	0.53	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002700.2	0.52	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0002698.2	0.51	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000553.3	0.50	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0002111.3	0.49	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000513.3	0.83	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000496.5	0.62	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000501.3	0.60	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000518.3	0.53	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002700.2	0.52	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0002698.2	0.51	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000553.3	0.50	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0002111.3	0.49	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BM199_RS00675

BM199_RS00670

Leader:

MDFVLDLQNLDAPEDMSYGDPSN

Core:

GGGSNLSLLASCANSTISLLTCH

Core with dehydrations:

GGGDhaNLDhaLLADhaCysANDhaDhbIDhaLLDhbCysH

antiSMASH-DB 4.0 matches for BM199_RS00670

100.0%

NZ_FOKG01000001.1and 1 other:

NZ_KB913032.1 Lanthipeptides: AMYAL_RS49265_lanthipeptide

Lanthipeptides: BM199_RS00670_lanthipeptide

1

GGGSNLSLLASCANSTISLLTCH

23

GGGSNLSLLASCANSTISLLTCH

1

GGGSNLSLLASCANSTISLLTCH

23

91.3%

NZ_FOWW01000003.1:

Lanthipeptides: BM051_RS10305_lanthipeptide

1

GGGSNLSLLASCANSTISLLTCH

23

GGGSNLSL+ASCANST+SLLTCH

1

GGGSNLSLVASCANSTVSLLTCH

23

87.5%

NZ_BMRB01000005.1and 1 other:

NZ_CP031087.1 Lanthipeptides: Actkin_RS09240_lanthipeptide

Lanthipeptides: IEY98_RS25135_lanthipeptide

1

GGGSNLSLLASCANSTISLLTCH

23

GG SNLSL+ASCANSTISLLTCH

2

GGASNLSLIASCANSTISLLTCH

24

with 87 more sequence matches

Only the first 10 are displayed here.

84.6%

NZ_RSEC01000008.1:

Lanthipeptides: EIY87_RS01555_lanthipeptide

1

GGGSNLSLLASCANSTISLLTCH

23

GG SNLSLLASCANSTISLLTCH

4

GGASNLSLLASCANSTISLLTCH

26

80.8%

NC_021252.1and 13 others:

NZ_CP008953.1 Lanthipeptides: AJAP_RS43890_lanthipeptideNZ_CP016174.1 Lanthipeptides: SD37_RS42925_lanthipeptideNZ_CP024972.1 Lanthipeptides: CVV72_RS27155_lanthipeptideNZ_CP054839.1 Lanthipeptides: HUO13_RS17795_lanthipeptideNZ_CP092497.1 Lanthipeptides: MJQ72_RS39855_lanthipeptideNZ_CP102415.1 Lanthipeptides: NQK81_RS21060_lanthipeptideNZ_CP120210.1 Lanthipeptides: P3102_RS01815_lanthipeptideNZ_FMZZ01000019.1 Lanthipeptides: BLS56_RS27445_lanthipeptideNZ_JPLW01000001.1 Lanthipeptides: ED92_RS42365_lanthipeptideNZ_LIWC01000002.1 Lanthipeptides: AMK34_RS42700_lanthipeptideNZ_LQCI01000034.1 Lanthipeptides: AVL48_RS39200_lanthipeptideNZ_LQMT02000022.1 Lanthipeptides: AVR91_RS42870_lanthipeptideNZ_LT629789.1 Lanthipeptides: BLU53_RS44680_lanthipeptide

Lanthipeptides: AORI_RS83200_lanthipeptide

1

GGGSNLSLLASCANSTISLLTCH

23

GG SNLSLLASCANST+SLLTCH

4

GGASNLSLLASCANSTVSLLTCH

26

80.8%

NZ_BNAY01000004.1and 1 other:

NZ_MUXN01000005.1 Lanthipeptides: B0293_RS43005_lanthipeptide

Lanthipeptides: IE302_RS20620_lanthipeptide

1

GGGSNLSLLASCANSTISLLTCH

23

GG SNLSLLASCA+STISLLTCH

4

GGTSNLSLLASCADSTISLLTCH

26

79.2%

NZ_CP031142.1:

Lanthipeptides: DL519_RS00755_lanthipeptide

1

GGGSNLSLLASCANSTISLLTCH

23

GG SNLSLLA+CA STISLLTC

2

GGASNLSLLAACAQSTISLLTCQ

24

78.6%

NZ_FNON01000002.1:

Lanthipeptides: BLV57_RS10465_lanthipeptide

1

GGG--SNLSLLASCANSTISLLTCH

23

GGG SNLSLLASC NSTISLLTCH

4

GGGEPSNLSLLASCTNSTISLLTCH

28

78.3%

NZ_CP095481.1:

Lanthipeptides: MUY22_RS14085_lanthipeptide

4

SNLSLLASCANSTISLLTCH

23

SNLSLLASC NST+SLLTCH

1

SNLSLLASCTNSTLSLLTCH

20

76.0%

NZ_CP017780.1and 1 other:

NZ_MQUQ01000015.1 Lanthipeptides: BS329_RS42815_lanthipeptide

Lanthipeptides: BKN51_RS36495_lanthipeptide

4

SNLSLLASCANSTISLLTCH

23

SNLSLLASC NSTISLLTCH

6

SNLSLLASCNNSTISLLTCH

25

75.0%

NZ_CP040605.1:

Lanthipeptides: FDZ84_RS37170_lanthipeptide

1

GGGSNLSLLASCANSTISLLTCH

23

GG SNLSLLA+CA ST+SLLTC

2

GGASNLSLLAACAQSTVSLLTCQ

24

75.0%

NZ_CP061007.1:

Lanthipeptides: IAG28_RS39520_lanthipeptide

1

GGGSNLSLLASCANSTISLLTCH

23

GG SNLSLLA+CA+ST+SLLTC

2

GGSSNLSLLAACAHSTVSLLTCQ

24

73.9%

NZ_CP059557.1:

Lanthipeptides: H2Q94_RS04010_lanthipeptide

3

GSNLSLLASCANSTISLLTCH

23

GSNLSLL +CANST+SLLTC+

3

GSNLSLLVACANSTVSLLTCN

23

MIBiG 4.0 matches for BM199_RS00670

21.7%

Lanthipeptide: SRO15-2212

15

STISLLTCH

23

S +SLL C

3

SQVSLLVCE

11

7.8%

BGC0002120.3 (NQE92413.1):

Lanthipeptide: nocathioamide A

16

TISLLTC

22

T+S L C

42

TLSSLPC

48

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 21.84 out of 21.84

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.24 out of 19.26

Confidence: medium

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 26.14 out of 30.22

Confidence: medium

Additional weak hits (15):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.62 out of 25.75

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.22 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.00 out of 18.57

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.63 out of 36.54

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.53 out of 39.62

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.78 out of 29.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.56 out of 35.63

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.75 out of 29.65

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.11 out of 25.75

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.51 out of 39.62

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.38 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.16 out of 27.18

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.60 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.94 out of 33.14

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

BM199_RS00670 - Class III

Cleavage pHMM score: 12.50

RODEO score: 0

Monoisotopic mass: 2092.1 Da

Molecular weight: 2093.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2111.4 Da N = 1; 2129.4 Da N = 2; 2147.4 Da N = 3; 2165.4 Da N = 4; 2183.5 Da N = 5

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
ClgR	Caseinolytic protease gene regulator
MexT	Global virulence regulator

NZ_FOKG01000010.1 - Region 1 - thioamitides

Download region SVG

Location: 14,485 - 37,018 nt. (total: 22,534 nt) Show pHMM detection rules used

thioamitides: ((YcaO or TIGR03604) and TfuA)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase reductase

BM199_RS17425: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

BM199_RS17455: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BM199_RS17490: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

Transferases: aminotransferase

BM199_RS17410: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Ligases: ligase

BM199_RS17475: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000894.4	0.50	other (other)	citrinin	Monascus aurantiacus
BGC0001849.4	0.47	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0000859.3	0.47	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000853.3	0.45	other (other)	ectoine	Streptomyces anulatus
BGC0000919.5	0.44	other (other)	phaseolotoxin	Pseudomonas syringae
BGC0000895.5	0.42	other (other)	citrulline	Lactobacillus plantarum subsp. plantarum
BGC0001696.5	0.42	ribosomal	neothioviridamide	Streptomyces sp.
BGC0001802.5	0.42	ribosomal	thioholgamide A, thioholgamide B	Streptomyces malaysiense
BGC0002382.3	0.41	ribosomal	grimoviridin	Grimontia marina
BGC0000854.3	0.41	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000894.4	0.50	other (other)	citrinin	Monascus aurantiacus
BGC0001849.4	0.47	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0000859.3	0.47	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000853.3	0.45	other (other)	ectoine	Streptomyces anulatus
BGC0000919.5	0.44	other (other)	phaseolotoxin	Pseudomonas syringae
BGC0000895.5	0.42	other (other)	citrulline	Lactobacillus plantarum subsp. plantarum
BGC0001696.5	0.42	ribosomal	neothioviridamide	Streptomyces sp.
BGC0001802.5	0.42	ribosomal	thioholgamide A, thioholgamide B	Streptomyces malaysiense
BGC0002382.3	0.41	ribosomal	grimoviridin	Grimontia marina
BGC0000854.3	0.41	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (1):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.99 out of 30.25

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_FOKG01000010.1 - Region 2 - ectoine

Download region SVG

Location: 68,188 - 78,580 nt. (total: 10,393 nt) Show pHMM detection rules used

ectoine: ectoine_synt

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000860.3	0.45	other (other)	ectoine	Methylarcula marina
BGC0000852.5	0.44	other (other)	ectoine	Sporosarcina pasteurii
BGC0000857.3	0.44	other (other)	ectoine	Methylophaga alcalica
BGC0000858.3	0.43	other (other)	ectoine	Methylobacter marinus
BGC0000859.3	0.42	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000854.3	0.40	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z
BGC0000853.3	0.39	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.39	other (ectoine)	ectoine	Streptomyces sp.
BGC0002064.2	0.27	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0001366.5	0.25	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000860.3	0.45	other (other)	ectoine	Methylarcula marina
BGC0000852.5	0.44	other (other)	ectoine	Sporosarcina pasteurii
BGC0000857.3	0.44	other (other)	ectoine	Methylophaga alcalica
BGC0000858.3	0.43	other (other)	ectoine	Methylobacter marinus
BGC0000859.3	0.42	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000854.3	0.40	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z
BGC0000853.3	0.39	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.39	other (ectoine)	ectoine	Streptomyces sp.
BGC0002064.2	0.27	ribosomal	felipeptin A2, felipeptin A1	Amycolatopsis sp. YIM 10
BGC0001366.5	0.25	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (2):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.68 out of 30.22

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_FOKG01000022.1 - Region 1 - T1PKS

Download region SVG

Location: 22,283 - 68,834 nt. (total: 46,552 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BM199_RS27495: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: esterase protease

BM199_RS27490: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

BM199_RS27520: putative esterase

smcogs: SMCOG1104: putative esterase

Isomerases: mutase racemase

BM199_RS27505: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

BM199_RS27555: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

Ligases: ligase synthase

BM199_RS27480: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BM199_RS27525: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BM199_RS27530: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BM199_RS27595: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Translocases: transport

BM199_RS27540: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002277.2	0.65	NRPS	atromentin	Suillus grevillei
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.76	PKS	asperlactone	Aspergillus ochraceus
BGC0001244.3	0.75	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002277.2	0.65	NRPS	atromentin	Suillus grevillei
BGC0001254.3	0.64	PKS	ACT-Toxin II	Alternaria alternata
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.52 out of 19.26

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.36 out of 20.97

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.36 out of 19.26

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.34 out of 20.97

Confidence: medium

Additional weak hits (7):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.30 out of 21.55

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 26.41 out of 39.62

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.10 out of 26.06

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.01 out of 20.97

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.01 out of 20.97

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.70 out of 26.33

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.72 out of 30.98

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 22282 - 68834:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BM199_RS27480: X

AMP-binding (57..456): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SFLAHGTGLSQLVLFVAGSHNLYGSTEVAVATVA
Nearest Stachelhaus code(s):: Tyr DTSTLAAVAK (44% 8Å match)
Stachelhaus code match:: 60% (weak)

BM199_RS27525: mmal

PKS_AT (624..898): mmal

ATSignature: Ethylmalonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Ethylmalonyl-CoA: 70.8%; Methylmalonyl-CoA: 70.8%; Propionyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 129.0
Ethylmalonyl-CoA: 119.6
Methoxymalonyl-CoA: 108.7
Propionyl-CoA: 79.5
Malonyl-CoA: 72.9
trans-1,2-CPDA: 71.2
2-Methylbutyryl-CoA: 61.7
CHC-CoA: 60.7
Isobutyryl-CoA: 57.6
Acetyl-CoA: 43.8
Benzoyl-CoA: 27.0
3-Methylbutyryl-CoA: 25.5
fatty_acid: 24.4
inactive: 24.3

BM199_RS27530: NH2

CAL_domain (171..498): NH2

minowa: NH2

Prediction, score:

NH2: 115.2
Acetyl-CoA: 53.9
fatty_acid: 37.3
shikimic_acid: 0.0
AHBA: 0.0

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FOKG01000005.1 - Region 1 - NRPS

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Location: 159,078 - 202,212 nt. (total: 43,135 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase reductase

BM199_RS10370: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

BM199_RS10500: alkyl hydroperoxide reductase/ Thiol specific

smcogs: SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific

Transferases: aminotransferase methyltransferase

BM199_RS10355: methyltransferase

smcogs: SMCOG1089: methyltransferase

BM199_RS10365: aminotransferase

smcogs: SMCOG1019: aminotransferase

Ligases: ligase

BM199_RS10385: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BM199_RS10395: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001261.3	0.67	NRPS	AM-toxin	Alternaria alternata
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.66	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.66	NRPS	HC-toxin	Alternaria jesenskae
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001261.3	0.67	NRPS	AM-toxin	Alternaria alternata
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.66	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.66	NRPS	HC-toxin	Alternaria jesenskae
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.40 out of 19.26

Confidence: medium

Additional weak hits (13):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.04 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.44 out of 27.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.83 out of 29.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.34 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.43 out of 27.78

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.77 out of 32.74

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.27 out of 30.22

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.68 out of 35.63

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.02 out of 29.65

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 21.55 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.65 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.00 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 159077 - 202212:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BM199_RS10385: X

AMP-binding (9..426): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FEVFFDGSILAGMSSFGGEINCYGPSECACIVTA
Nearest Stachelhaus code(s):: Met DGLILGICVK (50% 8Å match); n-oxoDec DGLTCGVIIK (38% 8Å match)
Stachelhaus code match:: 60% (weak)

BM199_RS10395: X

AMP-binding (467..857): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVCFQEPYLCVSGEVNHYGPTETQAVTTR
Nearest Stachelhaus code(s):: Pro DVQYCAMVVK (76% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein

NZ_FOKG01000003.1 - Region 1 - lassopeptide

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Location: 52,028 - 77,320 nt. (total: 25,293 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BM199_RS06470: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: aminotransferase glycosyltransferase

BM199_RS06490: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

BM199_RS06535: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001552.3	0.53	ribosomal	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001634.3	0.52	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0000575.5	0.51	ribosomal	lariatin	Rhodococcus jostii
BGC0001549.3	0.51	ribosomal	citrulassin B	Streptomyces aurantiacus JA 4570
BGC0001551.4	0.50	ribosomal	citrulassin E	Streptomyces glaucescens
BGC0001889.2	0.50	ribosomal	fusilassin	Thermobifida fusca YX
BGC0000571.3	0.49	ribosomal	burhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002450.2	0.42	other (other)	sarasinoside	uncultured bacterium
BGC0000623.3	0.42	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001552.3	0.53	ribosomal	citrulassin F	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001634.3	0.52	ribosomal	keywimysin	Streptomyces sp. NRRL F-5702
BGC0000575.5	0.51	ribosomal	lariatin	Rhodococcus jostii
BGC0001549.3	0.51	ribosomal	citrulassin B	Streptomyces aurantiacus JA 4570
BGC0001551.4	0.50	ribosomal	citrulassin E	Streptomyces glaucescens
BGC0001889.2	0.50	ribosomal	fusilassin	Thermobifida fusca YX
BGC0000571.3	0.49	ribosomal	burhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002450.2	0.42	other (other)	sarasinoside	uncultured bacterium
BGC0000623.3	0.42	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptides - No core peptides found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (8):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 21.46 out of 33.73

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.33 out of 30.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.79 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.17 out of 26.06

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.42 out of 26.12

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.29 out of 27.78

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.05 out of 20.97

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.42 out of 33.73

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 4 - 82; Score: 69.4

TFBS definitions

Regulator	Description

NZ_FOKG01000003.1 - Region 2 - hserlactone

Download region SVG

Location: 171,069 - 191,821 nt. (total: 20,753 nt) Show pHMM detection rules used

hserlactone: Autoind_synth

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BM199_RS07060: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BM199_RS07105: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acyltransferase formyltransferase glycosyltransferase

BM199_RS07045: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

BM199_RS07085: acyltransferase

smcogs: SMCOG1141: acyltransferase

BM199_RS07100: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

Hydrolases: hydrolase

BM199_RS07050: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Isomerases: mutase

BM199_RS07030: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.53	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001682.3	0.45	other (other)	N-acyl lysine, N-acyl ornithine	Pseudomonas aeruginosa LESB58
BGC0002439.3	0.40	other (other)	diastaphenazine, izumiphenazine C	Streptomyces diastaticus
BGC0000894.4	0.40	other (other)	citrinin	Monascus aurantiacus
BGC0000840.5	0.39	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0001302.5	0.38	other (other)	lomofungin	Streptomyces lomondensis
BGC0001521.3	0.37	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0000730.3	0.37	saccharide	capsular polysaccharide	Klebsiella pneumoniae
BGC0000731.3	0.37	saccharide	capsular polysaccharide	Klebsiella pneumoniae
BGC0001043.4	0.37	NRPS, PKS	SGR PTMs, SGR PTM Compound b, SGR PTM Compound c, SGR PTM Compound d	Streptomyces griseus subsp. griseus NBRC 13350

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.53	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001682.3	0.45	other (other)	N-acyl lysine, N-acyl ornithine	Pseudomonas aeruginosa LESB58
BGC0002439.3	0.40	other (other)	diastaphenazine, izumiphenazine C	Streptomyces diastaticus
BGC0000894.4	0.40	other (other)	citrinin	Monascus aurantiacus
BGC0000840.5	0.39	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0001302.5	0.38	other (other)	lomofungin	Streptomyces lomondensis
BGC0001521.3	0.37	NRPS	auriculamide	Herpetosiphon aurantiacus DSM 785
BGC0000730.3	0.37	saccharide	capsular polysaccharide	Klebsiella pneumoniae
BGC0000731.3	0.37	saccharide	capsular polysaccharide	Klebsiella pneumoniae
BGC0001043.4	0.37	NRPS, PKS	SGR PTMs, SGR PTM Compound b, SGR PTM Compound c, SGR PTM Compound d	Streptomyces griseus subsp. griseus NBRC 13350

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (6):

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.44 out of 36.53

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.78 out of 36.54

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.65 out of 27.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.98 out of 29.38

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.27 out of 32.93

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.41 out of 25.75

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_FOKG01000016.1 - Region 1 - redox-cofactor

Download region SVG

Location: 54,899 - 76,925 nt. (total: 22,027 nt) Show pHMM detection rules used

redox-cofactor: (cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

BM199_RS23835: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Transferases: acetyltransferase methyltransferase

BM199_RS23825: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

BM199_RS23890: methyltransferase

smcogs: SMCOG1089: methyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001173.5	0.38	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000528.5	0.37	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000626.3	0.37	ribosomal	thuricin	Bacillus thuringiensis
BGC0002015.4	0.36	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001727.3	0.36	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000122.5	0.36	PKS	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0000521.3	0.36	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis
BGC0001311.5	0.36	ribosomal	flavecins	Ruminococcus flavefaciens FD-1
BGC0000498.5	0.36	ribosomal	bovicin HJ50	Streptococcus equinus
BGC0001100.5	0.36	NRPS, PKS	lankacidin C	Streptomyces rochei

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001173.5	0.38	ribosomal	plantazolicin	Bacillus pumilus ATCC 7061
BGC0000528.5	0.37	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000626.3	0.37	ribosomal	thuricin	Bacillus thuringiensis
BGC0002015.4	0.36	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001727.3	0.36	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000122.5	0.36	PKS	phenylnannolone A, phenylnannolone B, phenylnannolone C	Nannocystis pusilla
BGC0000521.3	0.36	ribosomal	lacticin 481	Lactococcus lactis subsp. lactis
BGC0001311.5	0.36	ribosomal	flavecins	Ruminococcus flavefaciens FD-1
BGC0000498.5	0.36	ribosomal	bovicin HJ50	Streptococcus equinus
BGC0001100.5	0.36	NRPS, PKS	lankacidin C	Streptomyces rochei

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.57 out of 18.57

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.99 out of 20.97

Confidence: medium

Additional weak hits (4):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.67 out of 26.06

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.79 out of 20.97

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.53 out of 20.97

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.73 out of 27.04

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: PqqD_RRE; Location: 1 - 82; Score: 79.8

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
ToxT	Regulator of MSHA biosynthesis

NZ_FOKG01000002.1 - Region 1 - terpene

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Location: 332,070 - 353,659 nt. (total: 21,590 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Select a gene to view the details available for it

Gene overview

Tailoring

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KnownClusterBlast

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Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

BM199_RS05795: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

BM199_RS05810: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

Hydrolases: hydrolase

BM199_RS05785: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: synthase

BM199_RS05815: pseudouridine synthase

smcogs: SMCOG1279: pseudouridine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002043.3	0.55	ribosomal	cittilin A, cittilin B	Myxococcus xanthus DK 1622
BGC0001198.3	0.53	terpene	(+)-O-methylkolavelool	Herpetosiphon aurantiacus DSM 785
BGC0002610.3	0.49	terpene	stellatic acid	Aspergillus stellatus
BGC0002644.2	0.47	other (other), terpene	domoic acid, isodomoic acid A, isodomoic acid B, prekainic acid, 7'-carboxyN-geranyl-L-glutamic acid, isodomoic acid C	Chondria armata
BGC0001375.4	0.44	terpene	penitrem A, penitrem B, penitrem C, penitrem D, penitrem E, penitrem F	Penicillium simplicissimum
BGC0001528.4	0.43	terpene	betaestacin I, betaestacin II	Phoma betae
BGC0000286.3	0.43	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001485.4	0.42	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0001605.3	0.42	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001911.3	0.41	terpene	raimonol	Streptomyces anulatus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002043.3	0.55	ribosomal	cittilin A, cittilin B	Myxococcus xanthus DK 1622
BGC0001198.3	0.53	terpene	(+)-O-methylkolavelool	Herpetosiphon aurantiacus DSM 785
BGC0002610.3	0.49	terpene	stellatic acid	Aspergillus stellatus
BGC0002644.2	0.47	other (other), terpene	domoic acid, isodomoic acid A, isodomoic acid B, prekainic acid, 7'-carboxyN-geranyl-L-glutamic acid, isodomoic acid C	Chondria armata
BGC0001375.4	0.44	terpene	penitrem A, penitrem B, penitrem C, penitrem D, penitrem E, penitrem F	Penicillium simplicissimum
BGC0001528.4	0.43	terpene	betaestacin I, betaestacin II	Phoma betae
BGC0000286.3	0.43	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0001485.4	0.42	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0001605.3	0.42	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001911.3	0.41	terpene	raimonol	Streptomyces anulatus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
diterpene			C20	C6-C11 + C10-C15	PP

Genes with terpene domains:

BM199_RS05790: T2TS

Domain 1 (5 - 501): Type 2 terpene synthase

Subtype: Diterpene synthase

Reactions:: GGPP → diterpene_C6-C11/C10-C15_PP

Transcription Factor Binding Sites

Binding sites found:

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.43 out of 23.43

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.25 out of 20.47

Confidence: medium

Additional weak hits (4):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.92 out of 26.33

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.18 out of 29.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.34 out of 30.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.42 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
GGPP	Geranylgeranyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
BldD	Development and antibiotic global regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_FOKG01000006.1 - Region 1 - NAPAA,indole

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Location: 284,858 - 330,361 nt. (total: 45,504 nt) Show pHMM detection rules used

indole: (indsynth or dmat or indole_PTase)
NAPAA: (AMP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase glycosyltransferase

BM199_RS12735: sugar transferase

smcogs: SMCOG1146: sugar transferase

BM199_RS12845: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: protease

BM199_RS12800: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Ligases: synthase

BM199_RS12900: Pseudouridine synthase

smcogs: SMCOG1154: Pseudouridine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.48	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.27	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.26	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.26	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001641.4	0.26	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.26	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.26	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.25	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001197.5	0.25	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0002276.2	0.24	NRPS	choline	Aspergillus nidulans FGSC A4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.61	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.60	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.59	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002276.2	0.57	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001268.4	0.55	NRPS, PKS	fusarin C	Fusarium fujikuroi

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.23 out of 19.52

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.73 out of 23.79

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.85 out of 26.85

Confidence: medium

Additional weak hits (9):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.04 out of 21.55

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.39 out of 31.73

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.13 out of 30.22

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.95 out of 27.78

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.05 out of 20.97

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.11 out of 33.73

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.94 out of 32.74

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.95 out of 31.73

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.44 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 284857 - 330361:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 296446 - 330361:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BM199_RS12835: X

AMP-binding (41..412): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAELFDASCEELSTIFGGEWNTYGPTETTVVACA
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (68% 8Å match); Gln or Tyr DAESVGTVVK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
IolR	Regulator of the inositol catabolism
RutR	Regulator of the pyrimidine and purine metabolism

NZ_FOKG01000045.1 - Region 1 - lanthipeptide-class-i

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Location: 1 - 17,094 nt. (total: 17,094 nt) Show pHMM detection rules used

Region on contig edge.

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

BM199_RS32635: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

BM199_RS32640: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0001289.5	0.44	ribosomal	penisin	Paenibacillus ehimensis
BGC0002635.2	0.44	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000544.4	0.41	ribosomal	planosporicin	Planomonospora alba
BGC0001727.3	0.41	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000506.4	0.40	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.39	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0002120.3	0.38	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0001701.4	0.38	ribosomal	nisin O	Blautia obeum
BGC0000511.5	0.37	ribosomal	ericin S	Bacillus subtilis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0001289.5	0.44	ribosomal	penisin	Paenibacillus ehimensis
BGC0002635.2	0.44	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000544.4	0.41	ribosomal	planosporicin	Planomonospora alba
BGC0001727.3	0.41	ribosomal	paenilan	Paenibacillus polymyxa E681
BGC0000506.4	0.40	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.39	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0002120.3	0.38	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0001701.4	0.38	ribosomal	nisin O	Blautia obeum
BGC0000511.5	0.37	ribosomal	ericin S	Bacillus subtilis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.73 out of 18.46

Confidence: medium

Additional weak hits (6):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.93 out of 21.55

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.48 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.24 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.09 out of 29.38

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.95 out of 36.54

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 143 - 236; Score: 61.6

TFBS definitions

Regulator	Description
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FOKG01000009.1 - Region 1 - terpene-precursor

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Location: 160,966 - 182,057 nt. (total: 21,092 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

BM199_RS16715: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

BM199_RS16720: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Ligases: synthetase

BM199_RS16725: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.49	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.49	terpene	isorenieratene	Streptomyces argillaceus
BGC0000633.3	0.47	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.47	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000649.3	0.45	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000648.3	0.40	terpene	carotenoid	Myxococcus xanthus
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus
BGC0000678.5	0.38	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.49	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.49	terpene	isorenieratene	Streptomyces argillaceus
BGC0000633.3	0.47	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.47	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000649.3	0.45	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000648.3	0.40	terpene	carotenoid	Myxococcus xanthus
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus
BGC0000678.5	0.38	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

BM199_RS16725: PT_FPPS_like

Domain 1 (46 - 355): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.37 out of 20.47

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.33 out of 19.52

Confidence: strong

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 27.38 out of 29.65

Confidence: strong

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 24.78 out of 24.78

Confidence: strong

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.13 out of 20.47

Confidence: medium

Additional weak hits (8):

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.22 out of 30.98

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.51 out of 20.01

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.97 out of 39.62

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.75 out of 29.38

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.27 out of 20.01

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.59 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.76 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.38 out of 29.38

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
AfsR	Pleiotropic regulatory for antibiotic production
LexA variant 1	Repressor of DNA damage response
LexA variant 2	Repressor of DNA damage response
VqsM	Regulator of quorum-sensing signalling systems

NZ_FOKG01000023.1 - Region 1 - T1PKS,hglE-KS

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Location: 1 - 33,974 nt. (total: 33,974 nt) Show pHMM detection rules used

Region on contig edge.

hglE-KS: (hglE or hglD)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase electron transfer oxidoreductase

BM199_RS27760: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BM199_RS27765: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

BM199_RS27775: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

BM199_RS27820: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

BM199_RS27845: flavodoxin

smcogs: SMCOG1134: flavodoxin

Transferases: glycosyltransferase kinase

BM199_RS27735: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

BM199_RS27830: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Hydrolases: hydrolase

BM199_RS27740: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Ligases: synthase

BM199_RS27750: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BM199_RS27755: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.58	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.58	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.57	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.57	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	0.54	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.54	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.51	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.50	PKS	FR901512	fungal sp. No.14919
BGC0000041.3	0.49	PKS	oronofacic acid	Pseudomonas syringae
BGC0002276.2	0.45	NRPS	choline	Aspergillus nidulans FGSC A4

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.62	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.62	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.62	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	0.60	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.60	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.59	PKS	ACT-Toxin II	Alternaria alternata
BGC0002192.3	0.58	PKS	FR901512	fungal sp. No.14919
BGC0000041.3	0.58	PKS	oronofacic acid	Pseudomonas syringae
BGC0002276.2	0.55	NRPS	choline	Aspergillus nidulans FGSC A4

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.63 out of 18.46

Confidence: medium

Additional weak hits (12):

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.33 out of 36.54

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.63 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.34 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.06 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.62 out of 29.38

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.06 out of 29.65

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.48 out of 25.88

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.65 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.40 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.85 out of 28.19

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.48 out of 24.78

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 33974:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal)
Non-colinear ordering:: BM199_RS27750 + BM199_RS27745

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BM199_RS27750: mal

PKS_AT (635..845): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 4.4
trans-1,2-CPDA: 0.0
inactive: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Methylmalonyl-CoA: 0.0
Methoxymalonyl-CoA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

BM199_RS27755: mal

PKS_AT (1208..1505): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 97.7
inactive: 37.0
Methylmalonyl-CoA: 31.8
Methoxymalonyl-CoA: 28.8
CHC-CoA: 21.0
Ethylmalonyl-CoA: 17.4
Isobutyryl-CoA: 13.7
fatty_acid: 10.3
Propionyl-CoA: 4.6
Benzoyl-CoA: 3.4
2-Methylbutyryl-CoA: 2.8
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

TFBS definitions

Regulator	Description
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FOKG01000026.1 - Region 1 - NRP-metallophore,NRPS

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Location: 11,658 - 69,812 nt. (total: 58,155 nt) Show pHMM detection rules used

Region on contig edge.

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

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SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

BM199_RS29005: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

Transferases: formyltransferase kinase methyltransferase

BM199_RS28925: glycerol kinase

smcogs: SMCOG1210: glycerol kinase

BM199_RS28995: methyltransferase

smcogs: SMCOG1089: methyltransferase

BM199_RS29000: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

BM199_RS29060: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: ligase

BM199_RS29015: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002424.3	0.90	NRPS	saccharochelin A, saccharochelin B, saccharochelin C, saccharochelin D, saccharochelin E, saccharochelin F, saccharochelin G, saccharochelin H	Saccharothrix sp.
BGC0000349.5	0.86	NRPS	erythrochelin	Saccharopolyspora erythraea NRRL 2338
BGC0002135.2	0.86	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.75	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.74	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002424.3	0.75	NRPS	saccharochelin A, saccharochelin B, saccharochelin C, saccharochelin D, saccharochelin E, saccharochelin F, saccharochelin G, saccharochelin H	Saccharothrix sp.
BGC0000349.5	0.74	NRPS	erythrochelin	Saccharopolyspora erythraea NRRL 2338
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001135.5	0.70	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 26.84 out of 36.53

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.74 out of 20.47

Confidence: medium

Additional weak hits (5):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.03 out of 27.78

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.05 out of 19.26

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.89 out of 24.78

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.16 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.99 out of 36.53

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 11657 - 69812:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Fo-OH-Orn - D-X - OH-Orn - OH-Orn)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BM199_RS29015: Fo-OH-Orn - X - OH-Orn - OH-Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (466..861): Fo-OH-Orn

nrpys: Fo-OH-Orn

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LTAAFDVAVWDMILIVGTEVAAYGLTEATVNSTL
Nearest Stachelhaus code(s):: Fo-OH-Orn DVWIIGAVNK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1531..1938): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QWLYFDPSVQEGDLFSGGEYNVYGPTEAVVQVTG
Nearest Stachelhaus code(s):: Arg DPLDFGVVQK (79% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (3076..3484): OH-Orn

nrpys: OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: MNHVFDMSWEEWNVLLGGEYNLYGPTEYTINTLG
Nearest Stachelhaus code(s):: OH-Orn DMENLGLINK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4150..4556): OH-Orn

nrpys: OH-Orn

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LTAAFDVAVFEMILLVGTEIAAYGLTEATVNSTL
Nearest Stachelhaus code(s):: OH-Orn DVFILGAVNK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
DmdR1	Iron(II)-dependent repressor
VqsM	Regulator of quorum-sensing signalling systems

NZ_FOKG01000007.1 - Region 1 - RiPP-like,T1PKS,oligosaccharide

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Location: 8,132 - 98,235 nt. (total: 90,104 nt) Show pHMM detection rules used

Region on contig edge.

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
oligosaccharide: (repeatable(3, [DUF1205, Glyco_transf_28, Glycos_transf_1, Glycos_transf_2, MGT]) and minscore(MGT, 150))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase reductase

BM199_RS13090: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

BM199_RS13140: flavin reductase domain protein FMN-binding

smcogs: SMCOG1160: flavin reductase domain protein FMN-binding

BM199_RS13180: alkyl hydroperoxide reductase/ Thiol specific

smcogs: SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific

BM199_RS13200: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

BM199_RS13235: acyl-CoA dehydrogenase type 2

smcogs: SMCOG1191: acyl-CoA dehydrogenase type 2

BM199_RS13325: nucleotide sugar dehydrogenase

smcogs: SMCOG1152: nucleotide sugar dehydrogenase

BM199_RS13340: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

BM199_RS33865: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase aminotransferase glycosyltransferase methyltransferase transferase

BM199_RS13130: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BM199_RS13150: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

BM199_RS13195: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

BM199_RS13220: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

BM199_RS13230: aminotransferase

smcogs: SMCOG1019: aminotransferase

BM199_RS13260: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

BM199_RS13280: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

BM199_RS13295: methyltransferase

smcogs: SMCOG1248: methyltransferase

BM199_RS13300: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

BM199_RS13305: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

BM199_RS13315: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

BM199_RS13345: methyltransferase

smcogs: SMCOG1248: methyltransferase

BM199_RS13350: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

BM199_RS13360: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: hydrolase

BM199_RS13160: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

BM199_RS13250: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

BM199_RS13370: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: decarboxylase dehydratase

BM199_RS13215: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

BM199_RS13320: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Translocases: permease

BM199_RS13225: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000227.5	0.38	PKS	granaticin	Streptomyces violaceoruber
BGC0001374.5	0.38	other (other)	thienodolin	Streptomyces albogriseolus
BGC0000197.5	0.37	PKS, saccharide	aranciamycin	Streptomyces echinatus
BGC0000263.5	0.34	PKS	ravidomycin	Streptomyces ravidus
BGC0000201.5	0.33	PKS, saccharide	auricin	Streptomyces aureofaciens
BGC0000056.3	0.32	PKS	esperamicin	Actinomadura verrucosospora
BGC0000224.4	0.31	PKS	fredericamycin A	Streptomyces griseus
BGC0000273.5	0.31	PKS, saccharide	steffimycin D	Streptomyces steffisburgensis
BGC0000190.5	0.31	PKS	A-74528	Streptomyces sp. SANK 61196
BGC0000245.5	0.30	PKS	medermycin	Streptomyces sp. AM-7161

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000056.3	0.66	PKS	esperamicin	Actinomadura verrucosospora
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001817.5	0.56	other (other)	vancosamine	Streptomyces sp. WAC2288
BGC0001276.3	0.56	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001273.3	0.56	PKS	asperlactone	Aspergillus ochraceus
BGC0000278.3	0.55	PKS	urdamycin	Streptomyces fradiae
BGC0002192.3	0.55	PKS	FR901512	fungal sp. No.14919
BGC0001254.3	0.55	PKS	ACT-Toxin II	Alternaria alternata
BGC0002043.3	0.55	ribosomal	cittilin A, cittilin B	Myxococcus xanthus DK 1622
BGC0000660.5	0.52	terpene	albaflavenone	Streptomyces coelicolor A3(2)

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.90 out of 23.79

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

Additional weak hits (8):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.71 out of 25.75

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.62 out of 32.74

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.12 out of 27.55

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 21.74 out of 35.63

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.93 out of 24.78

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.59 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.08 out of 33.14

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.21 out of 20.01

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

RREFinder

TFBS Finder

NRPS/PKS substrate predictions

BM199_RS13130: mal

PKS_AT (621..850): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 58.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 77.1
Methoxymalonyl-CoA: 54.3
Methylmalonyl-CoA: 48.1
inactive: 31.2
Propionyl-CoA: 29.8
Ethylmalonyl-CoA: 26.8
Isobutyryl-CoA: 23.8
fatty_acid: 15.3
trans-1,2-CPDA: 14.5
2-Methylbutyryl-CoA: 13.9
CHC-CoA: 13.6
Benzoyl-CoA: 7.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

RRE predictions

: RRE type: Goadsporin_CD_RRE; Location: 10 - 108; Score: 75.1; RRE type: NHLP_CD_RRE; Location: 10 - 104; Score: 55.4; RRE type: Cyanobactin_D_RRE; Location: 8 - 73; Score: 53.0

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism

NZ_FOKG01000039.1 - Region 1 - terpene

Download region SVG

Location: 1 - 19,546 nt. (total: 19,546 nt) Show pHMM detection rules used

Region on contig edge.

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

BM199_RS31975: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

BM199_RS32000: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Transferases: methyltransferase

BM199_RS32020: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: esterase hydrolase

BM199_RS32065: thioesterase superfamily protein

smcogs: SMCOG1144: thioesterase superfamily protein

BM199_RS32080: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.59	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.56	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000636.3	0.52	terpene	carotenoid	Brevibacterium linens
BGC0000650.3	0.51	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0001227.3	0.48	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.48	terpene	carotenoid	Streptomyces avermitilis
BGC0000648.3	0.47	terpene	carotenoid	Myxococcus xanthus
BGC0000644.3	0.47	terpene	carotenoid	Dietzia sp. CQ4
BGC0000645.3	0.46	terpene	carotenoid	Halobacillus halophilus DSM 2266
BGC0000709.5	0.46	saccharide	neomycin	Streptomyces fradiae ATCC 10745 = DSM 40063

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.59	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.56	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000636.3	0.52	terpene	carotenoid	Brevibacterium linens
BGC0000650.3	0.51	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0001227.3	0.48	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000633.3	0.48	terpene	carotenoid	Streptomyces avermitilis
BGC0000648.3	0.47	terpene	carotenoid	Myxococcus xanthus
BGC0000644.3	0.47	terpene	carotenoid	Dietzia sp. CQ4
BGC0000645.3	0.46	terpene	carotenoid	Halobacillus halophilus DSM 2266
BGC0000709.5	0.46	saccharide	neomycin	Streptomyces fradiae ATCC 10745 = DSM 40063

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
tetraterpene	C40 carotenoid		C40	unknown	unknown

Genes with terpene domains:

BM199_RS32005: PT_phytoene_like

Domain 1 (16 - 313): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → PSPP; FPP → squalene; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

BM199_RS34830: Lycopene_cycl

Domain 1 (5 - 93): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

BM199_RS34835: Lycopene_cycl

Domain 1 (4 - 94): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.19 out of 23.79

Confidence: medium

Additional weak hits (1):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.38 out of 29.25

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
IolR	Regulator of the inositol catabolism

NZ_FOKG01000004.1 - Region 1 - NRPS,NRPS-like,betalactone

Download region SVG

Location: 1 - 66,089 nt. (total: 66,089 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

BM199_RS08085: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BM199_RS08100: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

BM199_RS08120: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: acyltransferase aminotransferase methyltransferase

BM199_RS08095: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

BM199_RS08115: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

BM199_RS08130: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: esterase hydrolase

BM199_RS08075: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

BM199_RS08080: thioesterase

smcogs: SMCOG1004: thioesterase

BM199_RS08155: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: ligase synthase

BM199_RS08090: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BM199_RS08110: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BM199_RS08145: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	1.22	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	1.14	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	1.14	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	1.11	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	1.10	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	1.09	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	1.09	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	1.07	NRPS	enniatin	Fusarium equiseti
BGC0001825.4	1.06	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	1.06	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.72	NRPS	AM-toxin	Alternaria alternata
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.71	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.71	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.71	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.87 out of 25.88

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.12 out of 23.79

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.06 out of 28.19

Confidence: medium

Additional weak hits (19):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.21 out of 30.97

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.89 out of 33.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.41 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.44 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.77 out of 29.38

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.62 out of 31.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.46 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.07 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.14 out of 28.14

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.11 out of 33.73

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.29 out of 25.75

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.95 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.27 out of 29.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.29 out of 31.73

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.25 out of 26.85

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.67 out of 32.74

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.17 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 66089:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Phe - D-Val - X) + (D-X - Ser) + (Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 41212:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Phe - D-Val - X) + (D-X - Ser) + (Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 4922 - 66089:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Phe - D-Val - X) + (D-X - Ser) + (Thr)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BM199_RS08090: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (321..708): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGNQIFGGEVNMFGITETTVHVTW
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

BM199_RS08110: X - Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (502..904): X

nrpys: Orn, Lys, Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IAWSVDLFFADRELSVGSEVNAYGGTEATVDSTV
Nearest Stachelhaus code(s):: ohEnd DLSVMGAVDK (76% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2003..2389): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFICSGEYNLYGPTEASVDVTY
Nearest Stachelhaus code(s):: Ser DVWHISLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

BM199_RS08115: Phe - Val - X

Search NORINE for peptide: strict or relaxed

AMP-binding (17..406): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LSQAFDASVWEMTLVVAGEINAYGPTETTVCATM
Nearest Stachelhaus code(s):: Phe DAWTVAAVCK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1055..1447): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TGTMWDGMSLEGCLIVGGDVNGYGPVESMIVATC
Nearest Stachelhaus code(s):: Val DGLYIGGIMK (47% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2535..2929): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LPGAFDASTFENVLVVGGDINVYGPTECTSITTV
Nearest Stachelhaus code(s):: Leu DALVLAVSIK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
PsrA	Oleic acid responsive regulator
ZuR variant 1	Zinc-responsive repressor

NZ_FOKG01000004.1 - Region 2 - NI-siderophore

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Location: 163,262 - 196,415 nt. (total: 33,154 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

BM199_RS08705: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BM199_RS08720: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

BM199_RS08820: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: methyltransferase

BM199_RS08735: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: deaminase

BM199_RS08755: ornithine cyclodeaminase

smcogs: SMCOG1158: ornithine cyclodeaminase

Lyases: decarboxylase

BM199_RS08775: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: synthase

BM199_RS08750: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000943.5	0.58	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0001408.5	0.58	other (other)	xanthoferrin	Xanthomonas oryzae pv. oryzae KACC 10331
BGC0000946.5	0.53	other (other)	vibrioferrin	Vibrio parahaemolyticus
BGC0002527.2	0.52	other (other)	vibrioferrin	Azotobacter vinelandii CA
BGC0000941.3	0.51	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001499.5	0.50	other (other)	aerobactin	Pantoea ananatis
BGC0001531.5	0.50	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0000942.4	0.49	other (other)	petrobactin	Bacillus anthracis str. Ames
BGC0002471.3	0.49	other (other)	baumannoferrin A, baumannoferrin B	Acinetobacter baumannii AYE
BGC0001498.5	0.47	other (other)	aerobactin	Xenorhabdus szentirmaii DSM 16338

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000943.5	0.58	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0001408.5	0.58	other (other)	xanthoferrin	Xanthomonas oryzae pv. oryzae KACC 10331
BGC0000946.5	0.53	other (other)	vibrioferrin	Vibrio parahaemolyticus
BGC0002527.2	0.52	other (other)	vibrioferrin	Azotobacter vinelandii CA
BGC0000941.3	0.51	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001499.5	0.50	other (other)	aerobactin	Pantoea ananatis
BGC0001531.5	0.50	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0000942.4	0.49	other (other)	petrobactin	Bacillus anthracis str. Ames
BGC0002471.3	0.49	other (other)	baumannoferrin A, baumannoferrin B	Acinetobacter baumannii AYE
BGC0001498.5	0.47	other (other)	aerobactin	Xenorhabdus szentirmaii DSM 16338

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.75 out of 28.19

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 27.73 out of 36.53

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.07 out of 24.78

Confidence: medium

Additional weak hits (8):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.19 out of 30.25

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.70 out of 21.55

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.68 out of 29.65

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.73 out of 36.53

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.25 out of 29.38

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.68 out of 26.85

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.03 out of 30.12

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.96 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
DmdR1	Iron(II)-dependent repressor
LexA variant 2	Repressor of DNA damage response
ZuR variant 1	Zinc-responsive repressor

NZ_FOKG01000025.1 - Region 1 - ectoine

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Location: 52,483 - 62,875 nt. (total: 10,393 nt) Show pHMM detection rules used

Region on contig edge.

ectoine: ectoine_synt

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase methyltransferase

BM199_RS28785: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

BM199_RS28795: methyltransferase

smcogs: SMCOG1089: methyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000853.3	0.82	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.81	other (ectoine)	ectoine	Streptomyces sp.
BGC0000859.3	0.71	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000857.3	0.68	other (other)	ectoine	Methylophaga alcalica
BGC0000858.3	0.66	other (other)	ectoine	Methylobacter marinus
BGC0000855.3	0.66	other (other)	ectoine	Methylomicrobium kenyense
BGC0000856.3	0.65	other (other)	ectoine	Methylophaga thalassica
BGC0000854.3	0.65	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z
BGC0000860.3	0.63	other (other)	ectoine	Methylarcula marina
BGC0000852.5	0.57	other (other)	ectoine	Sporosarcina pasteurii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000853.3	0.82	other (other)	ectoine	Streptomyces anulatus
BGC0002052.3	0.81	other (ectoine)	ectoine	Streptomyces sp.
BGC0000859.3	0.71	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000857.3	0.68	other (other)	ectoine	Methylophaga alcalica
BGC0000858.3	0.66	other (other)	ectoine	Methylobacter marinus
BGC0000855.3	0.66	other (other)	ectoine	Methylomicrobium kenyense
BGC0000856.3	0.65	other (other)	ectoine	Methylophaga thalassica
BGC0000854.3	0.65	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z
BGC0000860.3	0.63	other (other)	ectoine	Methylarcula marina
BGC0000852.5	0.57	other (other)	ectoine	Sporosarcina pasteurii

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.13 out of 20.47

Confidence: medium

Additional weak hits (1):

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 23.96 out of 30.12

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_FOKG01000036.1 - Region 1 - lanthipeptide-class-i

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Location: 1,820 - 28,319 nt. (total: 26,500 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

BM199_RS31620: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

BM199_RS31625: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000544.4	0.49	ribosomal	planosporicin	Planomonospora alba
BGC0002316.2	0.49	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001289.5	0.49	ribosomal	penisin	Paenibacillus ehimensis
BGC0002635.2	0.46	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000506.4	0.45	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000515.4	0.44	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000536.3	0.44	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.44	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0001789.3	0.44	ribosomal	suicin 90-1330	Streptococcus suis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002576.2	0.75	ribosomal	mechercharmycin	Thermoactinomyces sp.
BGC0000544.4	0.49	ribosomal	planosporicin	Planomonospora alba
BGC0002316.2	0.49	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0001289.5	0.49	ribosomal	penisin	Paenibacillus ehimensis
BGC0002635.2	0.46	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000506.4	0.45	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000515.4	0.44	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000536.3	0.44	ribosomal	nisin Q	Lactococcus lactis
BGC0000535.4	0.44	ribosomal	nisin A	Lactococcus lactis subsp. lactis
BGC0001789.3	0.44	ribosomal	suicin 90-1330	Streptococcus suis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BM199_RS31625

BM199_RS31630

Leader:

MTVQMEWSPTTVATPAAEVEMGSEFDLDITLVEVVDPA

Core:

HLINMTDDGCGTTCEKSTCISAA

Core with dehydrations:

HLINMDhbDDGCysGDhbDhbCysEKDhaDhbCysIDhaAA

antiSMASH-DB 4.0 matches for BM199_RS31630

100.0%

NZ_CM001439.1and 3 others:

NZ_CP015163.1 Lanthipeptides: A4R43_RS24965_lanthipeptideNZ_FOKG01000036.1 Lanthipeptides: BM199_RS31630_lanthipeptideNZ_JALMDK010000002.1 Lanthipeptides: MZC64_RS10125_lanthipeptide

Lanthipeptides: SACMADRAFT_RS28825_lanthipeptide

1

HLINMTDDGCGTTCEKSTCISAA

23

HLINMTDDGCGTTCEKSTCISAA

1

HLINMTDDGCGTTCEKSTCISAA

23

95.7%

NZ_BBOJ01000005.1and 2 others:

NZ_CM001439.1 Lanthipeptides: SACMADRAFT_RS29465_lanthipeptideNZ_CM001439.1 Lanthipeptides: SACMADRAFT_RS29465_lanthipeptide

Lanthipeptides: AOM45_RS49405_lanthipeptide

1

HLINMTDDGCGTTCEKSTCISAA

23

HLINMTDDGCG TCEKSTCISAA

1

HLINMTDDGCGHTCEKSTCISAA

23

95.7%

NZ_SWMS01000027.1:

Lanthipeptides: FCN18_RS31945_lanthipeptide

1

HLINMTDDGCGTTCEKSTCISAA

23

HLINMTDDGCG TCEKSTCISAA

1

HLINMTDDGCGQTCEKSTCISAA

23

with 177 more sequence matches

Only the first 10 are displayed here.

91.3%

NZ_FOUP01000006.1:

Lanthipeptides: BM016_RS18395_lanthipeptide

1

HLINMTDDGCGTTCEKSTCISAA

23

HLINMTDDGCG TCEKSTCIS+A

1

HLINMTDDGCGHTCEKSTCISSA

23

60.9%

NZ_AZXF01000017.1:

Lanthipeptides: B042_RS24865_lanthipeptide

3

INMTDDGCGTTCEKSTCISAA

23

IN+TDDGCGTTC STC+++

1

INLTDDGCGTTC-PSTCVTSV

20

60.9%

NZ_JAMXJO010000001.1:

Lanthipeptides: NGM33_RS06840_lanthipeptide

2

LINMTDDGCGTTCEKSTCISAA

23

LIN+TDDGCG++C S SAA

1

LINLTDDGCGSSCPNSCTTSAA

22

60.9%

NZ_QOIL01000028.1:

Lanthipeptides: DQ384_RS35715_lanthipeptide

2

LINMTDDGCGTTCEKSTCISA

22

LIN+TDDGCG+TC S C +A

1

LINLTDDGCGSTC-ASPCATA

20

56.5%

NC_007777.1and 1 other:

NC_007777.1 Lanthipeptides: FRANCCI3_RS10225_lanthipeptide

Lanthipeptides: FRANCCI3_RS10225_lanthipeptide

1

HLINMTDDGCGTTCEKS

17

LI MTDDGCGTTCE +

1

RLIRMTDDGCGTTCETA

17

56.5%

NZ_CP044407.1:

Lanthipeptides: F7P10_RS18930_lanthipeptide

2

LINMTDDGCGTTCEKSTCISAA

23

L+N+TDDGC +TC CIS A

2

LVNVTDDGCTSTC--GACISNA

21

56.5%

NZ_KB906876.1and 1 other:

NZ_VWVS01000014.1 Lanthipeptides: FYW99_RS19210_lanthipeptide

Lanthipeptides: G011_RS31385_lanthipeptide

2

LINMTDDGCGTTCEKSTCISA

22

LIN+TDDGCGT+C +TC+++

1

LINLTDDGCGTSC-PTTCVTS

20

56.5%

NZ_RBWU01000008.1:

Lanthipeptides: BZB76_RS31240_lanthipeptide

3

INMTDDGCGTTCEKSTCISAA

23

+N+TDDGC TC +S C SAA

1

VNLTDDGCNPTCPES-CTSAA

20

56.0%

NZ_CP031264.1and 1 other:

NZ_CP031264.1 Lanthipeptides: C7M71_RS17720_lanthipeptide

Lanthipeptides: C7M71_RS17720_lanthipeptide

2

LINMTDDGCGTTCE----KSTCISA

22

L+N T DGCG+TC+ STCIS

1

LLNDTGDGCGSTCQSACSNSTCISG

25

52.2%

NZ_BCBX01000061.1and 1 other:

NZ_BCBX01000061.1 Lanthipeptides: AP234_RS35640_lanthipeptide

Lanthipeptides: AP234_RS35640_lanthipeptide

1

HLINMTDDGCGTTCEKSTCIS

21

LI MTDDGC +TC + C+S

1

QLIRMTDDGCNSTC-ATACVS

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (2):

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.58 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.65 out of 20.01

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

BM199_RS31630 - Class I

Cleavage pHMM score: 1.70

RODEO score: 23

Monoisotopic mass: 2262.0 Da

Molecular weight: 2263.5 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2281.5 Da N = 1; 2299.6 Da N = 2; 2317.6 Da N = 3

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 161 - 253; Score: 63.6

TFBS definitions

Regulator	Description

NZ_FOKG01000013.1 - Region 1 - linaridin