LOCUS       NZ_FOKG01000025        10393 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Amycolatopsis marina strain CGMCC 4.3568, whole genome shotgun
            sequence.
ACCESSION   NZ_FOKG01000025
VERSION     NZ_FOKG01000025.1
KEYWORDS    WGS; RefSeq.
SOURCE      Amycolatopsis marina
  ORGANISM  Amycolatopsis marina
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900111885.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 01:41:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,708
            CDSs (total)                      :: 6,643
            Genes (coding)                    :: 6,572
            CDSs (with protein)               :: 6,572
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1 (5S, 16S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 22 of 71
            Pseudo Genes (incomplete)         :: 62 of 71
            Pseudo Genes (internal stop)      :: 4 of 71
            Pseudo Genes (multiple problems)  :: 15 of 71
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:06:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 52482
            Orig. end                         :: 62875
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOKG01000025.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5393](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5393)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(599..613)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 23.96"
                     /tool="antismash"
     gene            complement(779..3601)
                     /locus_tag="BM199_RS28770"
                     /old_locus_tag="SAMN05216266_12556"
     CDS             complement(779..3601)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985330.1"
                     /locus_tag="BM199_RS28770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216266_12556"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_245788860.1"
                     /transl_table=11
                     /translation="MNERDIRDGTGPDVRIQLLGPVQLLADGRAIPVGGPGVRGLLALL
                     ALDANKVVTLDDIIDALWGHEPPATARTIVHGNVSHLRRVLRSVYGEKRADSAESARIR
                     TAPPGYQLHVDPDRIDVHRARALLERAAVAPLALRADLLLEAFALWQGPVLGGVPQSVA
                     APALEELRLAVHGARVDADLELGRHAELIAELTPMVRENPAAERTVGQLMLALYRAGRR
                     ADALDIFRQVSRYVNDQLGIDPGPELRQLHDRLLQDDLDGGNPGVTVPLVPAQLPAALP
                     VLAGRSKELEWLDQLGAGADAGAGAIGVVTGTAGVGKSALVVWWAHRAAGQFVDGVLFA
                     SLRGFDPRTPALNPAEVLNQFLLGLGVAAAELPEQLHERVALYRSLLAGRRVLVVLDDA
                     GSAEQVRPLLPPGARSMTLVTSRSRLDGLTVSNTARVRALDVLAPADAVRLIEDLAGAT
                     PDGRNERLAKLCGYLPLALRIAGARLAGSPPWIGEELLKELADERTRLAALEADGGEAS
                     LRAALDLSYRGLAAEHAATLRVIGVLPLGAVDPHLIAALCGVEVGEARNRLRLLASQHL
                     LAEPRQDRFTPHDLVRLYVRDLAETELTGAERAELLADAVWYYQAVADRARRRLLRIVD
                     PLEFSGLLPEGSAVVPALATFDEALDWFAAEWANLLAVIEAASAAGAYEDAWQLARVAH
                     TYRVVRPWWDEWSRLVELGLTAAEAAGSELGRCWMLISRCAVALTFGLAEGSLTDAEAA
                     LDIARDLQDDRLRISAEIHVGCALVLAGRDDEAIARLLTAIEETERTGDLALQGQALNN
                     CAEAEKRAGRYSEAITHQLASLRVDRKLGDDSYAVVSLNNLAELHFALCEHAEAERYAR
                     EALELTVARGFVLQEGVARFTLGRILGARGNVSGARQQLTAALELHERVSPRSAVDIRA
                     ELERLRPADA"
     misc_feature    complement(911..913)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(3188..3190)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(3221..3223)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3991..4893)
                     /gene="thpD"
                     /locus_tag="BM199_RS28775"
                     /old_locus_tag="SAMN05216266_12557"
     CDS             complement(3991..4893)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="BM199_RS28775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216266_12557"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_091678124.1"
                     /sec_met_domain="PhyH (E-value: 1.3e-34, bitscore: 112.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTDTRVEDSYPTRIPGEAQLIDRVDPTVWGGESDGPIDAATLA
                     SHDAKGYSVVEGLLSPAEVQGYWQELVRLSSDDELKADERVITEKEFGEVRSIFEVHKI
                     SELIGELVRDPRVLDRARQILGSEVYIHQSRVNYMPGFKGSGFYWHSDFETWHAEDGMP
                     RPRSVSCSIALTDNYPFNGGLMVMPGSQRTFVQCVGETPDNYYKTSLKEQKIGVPSEND
                     IMKLAAECGIDQFTGPAGSALWFDSNIMHGSGNNITPYPRSNIFLVFNSVENALVQPYA
                     ASKPRPNFIGSRDFTPLSR"
     gene            complement(5001..5393)
                     /locus_tag="BM199_RS28780"
                     /old_locus_tag="SAMN05216266_12558"
     CDS             complement(5001..5393)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947387.1"
                     /locus_tag="BM199_RS28780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216266_12558"
                     /product="ectoine synthase"
                     /protein_id="WP_091678126.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.2e-57, bitscore:
                     184.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTLDEITDTDSDIKTENWRSKRIILAKEKAGFSVHETTLYAG
                     TVNDFWYANHIEAVFVFEGEGEITDKATGETHQLKPGSLYLLNNHDKHQVRPKTDMRTV
                     CVFNPPVTGREVHDENGVYPLVREED"
     gene            complement(5443..6696)
                     /gene="ectB"
                     /locus_tag="BM199_RS28785"
                     /old_locus_tag="SAMN05216266_12559"
     CDS             complement(5443..6696)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 1.3e-73.
                     Score: 239.9. Matches aSDomain:
                     nrpspksdomains_BM199_RS28785_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787752.1"
                     /locus_tag="BM199_RS28785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216266_12559"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_091678129.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7e-76, bitscore:
                     247.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFETLESEVRSYSRGWPVVFDRAQGSWQYAEDGKAYLDFFAGA
                     GALNYGHNNPVLKQALIDYISRDGVTHSLDMYTTAKRDFLQTLDDVLLKPRSMEYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHA
                     TPMPYDKYFDGAYPDFLYFDRLLQDSGSGLNEPAAVIVESVQGEGGINAARLEWLQGLS
                     ELCKRHGILLILDDVQMGCGRTGPFFSFEDAGIEPDMICLSKSISGYGLPMAVTLIKPE
                     LDVWEPGEHNGTFRGINPAFVTAAESLRTYWRDDELEKATRAKGERVGAALSEIADSYP
                     DAGLSAKGRGLARGLEFPNGELAGKVCAAAFERGLLMETSGPDGEVMKLLPPLTTTDAE
                     FTQGLEIITESVRTVLSV"
     aSDomain        complement(5623..6633)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM199_RS28785_Aminotran_3.1"
                     /evalue="1.30E-73"
                     /label="BM199_RS28785_Aminotran_3.1"
                     /locus_tag="BM199_RS28785"
                     /protein_end="358"
                     /protein_start="21"
                     /score="239.9"
                     /tool="antismash"
                     /translation="FDRAQGSWQYAEDGKAYLDFFAGAGALNYGHNNPVLKQALIDYIS
                     RDGVTHSLDMYTTAKRDFLQTLDDVLLKPRSMEYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPYDKYFDGAYPDFLYFDR
                     LLQDSGSGLNEPAAVIVESVQGEGGINAARLEWLQGLSELCKRHGILLILDDVQMGCGR
                     TGPFFSFEDAGIEPDMICLSKSISGYGLPMAVTLIKPELDVWEPGEHNGTFRGINPAFV
                     TAAESLRTYWRDDELEKATRAKGERVGAALSEIADSYPDAGLSAKGRGLARGLEFP"
     gene            complement(6747..7298)
                     /gene="ectA"
                     /locus_tag="BM199_RS28790"
                     /old_locus_tag="SAMN05216266_12560"
     CDS             complement(6747..7298)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466527.1"
                     /locus_tag="BM199_RS28790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216266_12560"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_091678131.1"
                     /transl_table=11
                     /translation="MSGKHSDRANGDNRAVGDVVIGNPSKSDGAALWRIARDSQKLDLN
                     SSYAYLLWCHDFASTSVVARVDGEPVGFVAGYRRPDAPDAVVVWQVAVDSSQRGRGLAG
                     KLLDALFDGLVGGGGVRYLETTITPDNEASIRLFASFAKRWDAELKSSALFAADDFPDE
                     HEPEDHYLIGPLAPRNVASG"
     gene            7800..8435
                     /locus_tag="BM199_RS28795"
                     /old_locus_tag="SAMN05216266_12561"
     CDS             7800..8435
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: oMT (38-138). E-value: 1.3e-14. Score:
                     45.0. Matches aSDomain: nrpspksdomains_BM199_RS28795_oMT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662664.1"
                     /locus_tag="BM199_RS28795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216266_12561"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_091678134.1"
                     /transl_table=11
                     /translation="MTTPDAGALFDGLAPGYDGDHFHESVAEALVRGLPATVSPRLVLD
                     VATGTGAAAFAALRHLDARRLVGVDISPAMIERARGRAAVQDADGRIDWQVGQAVPAPM
                     ADDAVDIVLCASSLHFLGRAALHDWLRVLRQGGRVAFTLPPAETFRPSGAFAKLVATDL
                     PLPQDNGQAAALATAAGFEQANATRLDITGERPRSVFLVHASAPSRTA"
     aSDomain        7914..8213
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BM199_RS28795_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="1.30E-14"
                     /label="BM199_RS28795_oMT.1"
                     /locus_tag="BM199_RS28795"
                     /protein_end="138"
                     /protein_start="38"
                     /score="45.0"
                     /tool="antismash"
                     /translation="SPRLVLDVATGTGAAAFAALRHLDARRLVGVDISPAMIERARGRA
                     AVQDADGRIDWQVGQAVPAPMADDAVDIVLCASSLHFLGRAALHDWLRVLRQGGR"
     gene            complement(8436..8900)
                     /locus_tag="BM199_RS28800"
                     /old_locus_tag="SAMN05216266_12562"
     CDS             complement(8436..8900)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005161302.1"
                     /locus_tag="BM199_RS28800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216266_12562"
                     /product="VOC family protein"
                     /protein_id="WP_091678137.1"
                     /transl_table=11
                     /translation="MNNFYHLCFVVQDVRKATEDLTRTVGVTWSPIREGRLGSWEYEIV
                     FSVEGPPFFEIIQGPAGSPWDATMGSRFDHIGYWSADVQSDKDRLAARGAPIEFDSCPY
                     GRSFTYHRLESLGTRIELVDQAVQEGFLRTWQPGGSAMPALNLDPPGAER"
     gene            9016..9588
                     /locus_tag="BM199_RS28805"
                     /old_locus_tag="SAMN05216266_12563"
     CDS             9016..9588
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668277.1"
                     /locus_tag="BM199_RS28805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216266_12563"
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="WP_091678139.1"
                     /transl_table=11
                     /translation="MPKKLRLTGDAEADKLLSEDPFALLTGMLLDQQIAMEVAFNGPKK
                     IADRMDGFSVTKIAEADLEEFVELCVQKPAIHRYGGSMARRVHALAEHIVENYGGKTDR
                     IWKTGKPDGAEALKRLKALPGFGDQKARIFLALLGKQLGVKLDGWREAAGAYGEEGSRR
                     SIADVTSAESLTEVREFKKAAKAAAKK"
     misc_feature    10222..10236
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.13"
                     /tool="antismash"
ORIGIN
        1 tgcagagcag ccttcgcgag tcccgtgagg acgtcggcta cgagactgtg accggcaggc
       61 tcgacggtgt gcgtacccgt cggcgcatct tctatctgga acgtgacacc ggcggaacca
      121 tccagggcgc tgtcgcgccc gacctgctcg acgagatcaa gctgaagctg gatcagccag
      181 tcacggcgcg cctgcgggtg gttcggacaa cgacgatcga cggccgccgc ggcaggccgg
      241 tgtacgagct gctggagatc cgctcccagg ggagtctgtt cgactgaggc gggcacaaaa
      301 cctcaccaca acgacaccca tccgccgaag gagacgcacg cagcgcgcag cctgcgaagc
      361 gctgcaaaat aaacacagcc tgaggcgtag cgatctccgc acccagccct acctcgcact
      421 ttcccgggaa agtgcgagcc ggcacgccct tgacctggta cgggagtttc catccccgca
      481 ctttcccggg aaagtgcggg cttgggttgg gggtacttga cgacgatgtc gatcttgctt
      541 gggggtactt gcaggcgcac catccccacg cgactggccg gccggaactc cccccaccag
      601 ggccggccgg ccagcacgca acggctcgtc tcgtgagacg cccgtcttcc tccgcgcagc
      661 cctccctgcg tcacgaagga acagtgccgc cacagcaact cctccccctg aaggccgctg
      721 tggcaagcac tgcgattcgg acagtacgcc gaacgatcgt gacgccacta agaatccgct
      781 aagcatcagc gggccgcagc cgctccagtt ctgcccggat gtcgaccgct gagcgcgggc
      841 ttactcgttc gtggagttcg agtgcggccg tgagctgctg cctcgctccc gatacgttgc
      901 cacgagcacc taagatccgg ccaagagtga agcgcgccac accctcttgc aaaacgaatc
      961 cgcgcgcgac cgtgagttcg agggcctccc gggcgtagcg ttccgcctcg gcatgctcgc
     1021 agagggcgaa gtgcagctcg gccaggttgt tcagcgacac aaccgcgtag ctgtcgtcgc
     1081 cgagcttgcg gtccacccgc agcgaagcca gttggtgcgt gatcgcctcg gagtacctgc
     1141 ccgcacgttt ctcggcctcc gcgcagttgt tcagcgcctg gccctgcagc gcgagatccc
     1201 cggttcgttc ggtctcctcg atcgcggtca acagcctggc gatcgcctcg tcgtcccggc
     1261 ccgcgagcac aagcgcgcag ccgacgtgga tttccgccga gatccgcagc cggtcgtcct
     1321 ggagatcccg cgcgatgtcc agtgcggcct cggcatcggt gaggctgcct tcggcgaggc
     1381 cgaaggtcag tgccaccgca caccgggaga tcagcatcca gcagcgcccc agttcgctgc
     1441 ccgccgcctc ggccgcggtc aacccgagct ccacgagccg ggaccactcg tcccaccacg
     1501 ggcgcaccac gcgataggtg tgcgccactc tcgccaactg ccacgcgtcc tcgtacgcac
     1561 cggcagcact ggcagcctcg atcacagcca gcagattggc ccattccgcg gcgaaccagt
     1621 ccagcgcttc gtcgaacgtg gccagcgcgg gcaccacagc actgccctcc ggcagcagtc
     1681 cggagaactc gagcgggtcc acgatccgca gcagccgcct gcgcgcacga tccgcgacgg
     1741 cctggtagta ccagaccgca tcggccagca gttccgctcg ttccgccccg gtcagctcgg
     1801 tctcggcgag gtcccgcacg tacagccgca ccagatcgtg cggggtgaaa cggtcctgcc
     1861 tcggctccgc cagcaggtgc tgggacgcca gcagccgcag cctgttccgg gcctcgccca
     1921 cctccacgcc acacagcgcg gcgatcaggt gtgggtccac ggcgccgagt ggcaggaccc
     1981 cgatgacccg caaggtcgca gcgtgctccg cggccagacc ccgataggac agatcgagtg
     2041 ccgcccgcaa actcgcctcc ccaccgtccg cctccagagc cgccagcctg gtccgttcgt
     2101 cggcgagttc cttcagcagt tcctcgccga tccacggtgg actaccggcg agccgagccc
     2161 ccgcgatccg caacgccagc gggagatagc cgcacagctt cgcgagccgc tcgttgcggc
     2221 cgtcgggagt cgcgcccgcc aggtcctcga tcaggcgcac cgcatcggcg ggagccagga
     2281 cgtccagcgc acgcacccgc gcggtgttgc tgaccgtgag gccgtccagc ctggaccggc
     2341 tggtcaccaa cgtcatcgac cgggctccgg gtggcagcaa cgggcgcacc tgctccgccg
     2401 atcccgcgtc gtccagcacc accagcaccc tgcgaccggc cagcagtgac cggtagagcg
     2461 cgacccgctc gtgcagctgt tccggtagct ccgccgcggc gacaccgaga ccgaggagga
     2521 actggttgag cacctcggca gggttgagcg cgggtgtgcg ggggtcgaat ccgcgcagcg
     2581 aggcgaagag cacgccgtcc acgaactgcc ccgcggctcg gtgcgcccac cagaccacga
     2641 gtgcgctctt cccgactccc gctgtcccgg tgaccacgcc gatcgcccct gcccctgcgt
     2701 cggcccccgc accgagctgg tcgagccact ccagctcttt gcttcgcccc gcgaggacgg
     2761 gcagcgcggc gggcagctgg gcgggaacca gtggaaccgt gaccccggga ttgccaccgt
     2821 ccaggtcgtc ctgcagcaac cggtcgtgta gctgccgcag ctcaggtcct gggtcgatgc
     2881 ccagctggtc gttcacgtac cgcgacacct gacggaagat gtcgagagca tcagcccttc
     2941 tgcccgcccg gtacagcgcg agcatgagct ggcccaccgt gcgctcggct gccgggttct
     3001 cccgcaccat cggcgtgagt tccgcgatca actcggcatg tctgcccagt tcgagatcgg
     3061 catcgactcg cgcaccgtgc acggccagcc gcagctcttc gagcgcgggc gccgccaccg
     3121 actgcgggac tccgccgagc accggccctt gccacagggc gaacgcctcc agcagcaggt
     3181 cggcccgtaa ggccagcggc gcaaccgcgg ctcgctcgag taacgctcgc gcgcggtgta
     3241 cgtcgatccg atctgggtcg acgtgcaact ggtaacccgg cggcgccgtg cggatccgag
     3301 cggactccgc gctgtccgcg cgtttctccc cgtacaccga tctcagcacg cgccgcagat
     3361 gggacacgtt gccgtgcacg atcgtccgcg ccgtcgcggg cggctcgtgt ccccacagcg
     3421 cgtcgatgat gtcgtcgagc gtcacgacct tgttggcatc gagtgcgagc agcgcaagca
     3481 gaccgcgaac gcccggtccc cctaccggta tcgcccttcc gtccgccagc agctgcaccg
     3541 ggccgagcag ctggatccgc acatccggcc ctgtgccatc acgaatatcc cgttcgttca
     3601 cgtccgtccc cccggtcggc cttcaacgag aagccgaacg tatcgcgaac gcggggacgg
     3661 cggatgcgca aaggcgattc cccgggactc caccaccgat cgtcacccgt tcggcggcag
     3721 cgcgcacaaa tacggccccg tgccatcagt cccgatctga tggcgcgggg ccgtactcgg
     3781 tggtgattgc ggcggactcg caggacgatc caccaggtgt tgtcgtaggc agcggcgcgg
     3841 gagctcaccc cgagcagaaa cccgttccac cgtcaccgac aacagtcacg ctgacgcccg
     3901 tccagccgga ccggcgcgcg tgaccggcac aacataacag ggtgcggtcg gaccgctaca
     3961 agcggcccga ccgcaccctt ggcgcccgcc ctaacgggac agcggagtga agtcccggct
     4021 gccgatgaag ttgggccggg gcttgctcgc cgcgtagggc tgcaccagcg cgttctccac
     4081 gctgttgaac accaggaaga tgttcgagcg cggatacggc gtgatgttgt tgcccgagcc
     4141 gtgcatgatg ttcgagtcga accagagcgc cgaaccggca ggtccggtga actggtcgat
     4201 cccgcactcg gcggcgagct tcatgatgtc gttctcgctc ggaacaccga tcttctgttc
     4261 cttgagcgag gtcttgtagt agttgtcagg tgtttcgccg acacactgca cgaaagtgcg
     4321 ctgcgagccg ggcatcacca tcaagccccc gttgaacggg tagttgtcgg tgagagcgat
     4381 cgagcagctc accgaccgag ggcgtggcat gccgtcctcg gcatgccagg tctcgaagtc
     4441 ggagtgccag tagaaaccgg accccttgaa gccgggcatg tagttcaccc ggctctggtg
     4501 gatgtacacc tccgagccga gaatctgccg agcccggtcg agcacccttg ggtcccgcac
     4561 cagctcgccg atcagttcac tgatcttgtg cacctcgaag atggaacgga cttcgccgaa
     4621 ctccttctcg gtgatgacgc gctcgtctgc cttgagctcg tcgtccgagg acaaccggac
     4681 cagttcctgc cagtagccct ggacctccgc aggcgagagc agtccttcca cgaccgagta
     4741 tcccttggca tcatgtgagg caagggtggc cgcatcgatc ggtccatcgg attcgccgcc
     4801 ccagacggtc ggatccacgc ggtcgatgag ctgtgcctcg cccgggattc tggtcgggta
     4861 actgtcctcc acacgggtgt ccgtgagcgt caagctgctc gcctcctaaa ttttattgtt
     4921 ttgaattgcc ccattcattg gtggtggtga ggagcaccgg tcagttcggg tggtctgtgg
     4981 tcggaacggc aggagccagc tcagtcctct tcgcgcacga gcgggtacac gccgttctcg
     5041 tcgtgtacct cccgtcccgt caccggcggg ttgaacacgc acacggtgcg catgtcggtc
     5101 ttcggccgca cctggtgctt gtcgtggttg ttcagcaggt acagcgatcc aggcttcagc
     5161 tgatgagtct caccggtggc cttgtcggtg atttccccct caccctcgaa aacaaacacc
     5221 gcttcgatgt ggttcgcgta ccaaaaatcg ttgaccgttc ccgcgtagag ggtggtctcg
     5281 tgcaccgaga atccagcctt ctccttggcc agaatgatgc gcttgctccg ccagttctcc
     5341 gtcttgatgt cggagtcggt gtcggtgatc tcgtcgaggg tgcggacgat caaagcgtgc
     5401 tccttgaggt gtcgttcagt tttgttgccc ggaaggactg tctcagacgg acagtacggt
     5461 ccggacggac tcggtgatga tctccaggcc ctgggtgaac tccgcgtccg tcgtggtcag
     5521 cggcggcagc agtttcatga cctcaccgtc ggggccagaa gtctccatga gcaggccacg
     5581 ctcgaaggcg gcggcgcaca ccttgcccgc gagctccccg ttggggaact cgagcccacg
     5641 cgcgagcccg cggcccttgg ccgagagtcc cgcgtccggg tacgagtcgg cgatctccga
     5701 cagtgcggca ccgacgcgct cgcccttcgc ccttgtcgcc ttctccagct cgtcgtcgcg
     5761 ccagtaggtg cgcagcgact ccgcggcggt gacgaaggcg ggattgatgc cccggaacgt
     5821 gccgttgtgc tcgcccggct cccagacatc gagttccggc ttgatcaggg tcacggccat
     5881 cggcaggccg tagccactga tggacttgga cagacagatc atgtccggct cgatgcccgc
     5941 gtcctcgaag ctgaagaacg ggcctgtccg gccgcagccc atctggacgt cgtcgaggat
     6001 cagcaggatg ccgtgccgct tgcacagctc ggaaaggccc tgcagccact ccaggcgcgc
     6061 ggcgttgatg ccgccttcac cctggacgct ctccacgatg acggcagcgg gctcgttcag
     6121 tccactaccc gagtcctgca gcaggcggtc gaagtagagg aagtcggggt aggcgccgtc
     6181 gaagtacttg tcgtacggca tcggggtcgc gtggaccagc ggaatgccgg cacccccgcg
     6241 cttcatggag ttaccggtga ccgaaagggc acccagggtc atcccgtgga acgcgttggt
     6301 gaagttgatg accgactcct tgccggtcac cttgcgcgcg agcttcagcg cggcttccac
     6361 cgcgttcgcg ccaccgggtc caggaaagac gaccttgtac tccatcgagc gcggcttcag
     6421 cagcacgtcg tcgagggtct gcaggaagtc tcgcttcgct gtcgtgtaca tgtccagtga
     6481 gtgggtgacc ccgtcacggg agatgtagtc gatcagcgcc tgcttcagca ccgggttgtt
     6541 gtgcccgtag ttgagcgccc ccgctccggc gaagaagtca aggtaggcct tgccgtcctc
     6601 ggcgtactgc cagctacctt gcgctcgatc gaacaccacg ggccagccac gactgtagct
     6661 gcgcacttcc gactcgagcg tttcgaagat gctcacgctt gctccgtctc tcagttctcg
     6721 tatcagcatt gtcccctgtg gaggactcag ccgcttgcca cgttccgggg cgccaacggc
     6781 ccgatcaggt agtggtcctc cggctcgtgt tcgtccggga agtcgtcggc cgcgaacagc
     6841 gccgacgact tcaactcggc gtcccagcgc ttcgcgaatg acgcgaacag ccgaatcgag
     6901 gcctcgttgt ccggggtgat cgtcgtctcc aggtagcgga ccccaccgcc gccgacgagc
     6961 ccgtcgaaca gcgcatccag caacttcccc gcgagaccac gtcctcgttg cgacgagtcc
     7021 accgccacct gccacacgac gaccgcatcc ggcgcgtctg gtctgcggta gccggccacg
     7081 aaaccgacgg gctcgccgtc gactctggcg acgactgagg tggaggcgaa atcgtggcac
     7141 cacaagaggt aggcgtatga cgagttgaga tccagctttt gtgaatctcg cgcaattcgc
     7201 caaagcgccg cgccgtcgga cttggacggg ttcccgatca ccacgtcgcc gaccgctcga
     7261 ttatcgccgt tggcacggtc tgagtgcttt ccggacatat caatgcaact taacagacca
     7321 gtttccgcag ctcagcccac gccacaacga gcaagcgagc tacctgcgaa gatgacgaat
     7381 cacctgtgac aatgacgaca tagatctacc gatcatgccc gttcgtgtgc tatgtgcgca
     7441 aaacgaccgg ccgccgtact cgccagcggc gtgcaggacc gtgaccagca ccgaggctcc
     7501 atctgccgct tacggtagtg gtatcgccac agaaatgcga gcgctacgac ggacgattcc
     7561 cgctgttcca cggcagccat ggcgacggag tgtgaccaag cggtccggga ccgctattag
     7621 cttgcccata ttctttccca gtcgtgcacc ttttcggaaa cgtgatctgc ccgccatcgc
     7681 agcgtcactt accgttcgac aatttccggg ccttcaccag taaatagtga aatgggccac
     7741 tcgcatcctt attacttccg ctatatcaca ccgtcgggcg gattcggtaa tcttcgatca
     7801 tgacgactcc ggatgccggt gctctgttcg acggtctcgc tcccggctac gacggcgacc
     7861 actttcacga gtccgtcgcc gaggcactcg tccgcgggct tcccgcgacg gtctccccgc
     7921 gactggtgct ggacgtggcc accggtaccg gtgcggcggc gttcgccgcg ctgcgccacc
     7981 tggacgcgcg caggctcgtc ggcgtcgaca tctcccccgc gatgatcgag cgggcgcgtg
     8041 gccgggctgc cgtgcaggac gcggacgggc ggatcgactg gcaggtcggc caggcggtcc
     8101 cggcgccgat ggcagacgac gcggtcgaca tcgtgctctg cgcctcgtcg ctgcacttcc
     8161 tcggcagagc ggcgcttcac gactggcttc gtgtcctgcg tcagggcggc cgggtggcct
     8221 tcaccctgcc gcctgccgag accttccgcc cgtccggcgc cttcgccaag ctcgtggcca
     8281 ccgacctgcc gcttccacag gacaacgggc aggcagcggc gctcgccacc gcggccgggt
     8341 tcgaacaggc caacgcgacc agactggaca tcaccgggga gcggccgagg tccgtcttcc
     8401 tcgtgcacgc gagcgcgccg tcccgcactg cttgatcagc gctctgcacc tggcgggtcg
     8461 aggttgagcg cgggcatggc ggagccgccc ggttgccagg tccgcaggaa gccctcctga
     8521 accgcctggt cgaccaactc gatccgggtg ccgaggctct ccaggcggtg gtaggtgaag
     8581 gaacggccat acgggcagga gtcgaactcg atcggggcgc ctcgggccgc cagccgatcc
     8641 ttgtccgact ggacgtccgc cgaccagtag ccgatgtggt cgaaccggga ccccatggtc
     8701 gcgtcccacg ggctacctgc gggaccttgg atgatctcga agaacggtgg tccctcgacg
     8761 gagaagacga tctcgtactc ccaggagccc agtcgcccct cccggatggg actccacgtc
     8821 acccccacgg tgcgcgtgag gtcctcggtc gcctttcgca catcctgcac gacgaaacac
     8881 aggtggtaga agttgttcac cgaccccttc taacagctcg cacagtccca cctcatggca
     8941 ggaccggccc caacgaaagc accgtcagcg atgtgggtca tggtgagccg gggatcgccg
     9001 tgccaggatg acgtcatgcc caagaagcta cgactgaccg gcgacgcaga ggccgacaag
     9061 ctgctcagcg aggacccgtt cgccttgctc acggggatgt tgctggatca gcagatcgcg
     9121 atggaggtcg ccttcaacgg gccgaagaag atcgccgacc ggatggacgg gttcagcgtc
     9181 acgaagatcg ccgaggctga tctcgaggag ttcgtggagt tgtgcgtgca gaaaccggcc
     9241 atccaccgct acggcggttc gatggctcgc agggtgcacg cgctcgcgga gcacatcgtg
     9301 gagaactacg gcgggaagac cgaccggatc tggaagacgg gcaagcccga cggtgccgaa
     9361 gcactgaagc gactcaaggc gctgccaggg ttcggcgacc agaaggcccg catcttcctc
     9421 gcgctcctcg gcaaacagct cggcgtcaag ctcgatgggt ggcgcgaggc ggcaggcgca
     9481 tacggcgaag agggctctcg ccggtccatt gcggacgtga ccagcgccga gtccctgacc
     9541 gaggtccgcg agttcaagaa ggcggcgaag gccgcggcca agaagtagcg gcttgcacac
     9601 ggcacaggtc tcagccactg aactggagca ggacctgcac ctgtagctca ccgacggccg
     9661 cgccgagcag cgcgccggtg gcgatgagaa tccactcgtc ctgctggaac gcgggtcgca
     9721 gcagcccctc gaactcctcg gcgtcgagtt ccttcatctt ccgcacgagg acgttgcgga
     9781 tgtccatcgc gtcctcggcg tagtcctcga cgtaacgcat ggtctcgggc aatctgcgca
     9841 tgatctgctc ggagatcccc agcttcatct cctggtagcg cttgctgccg acagcgagaa
     9901 ccaccagtgg tttggcgacg ctgacgtgct tggccagttc ctcgtccacc tgccgctgga
     9961 tcagcctgag caccctgtcc gacagcggtc cccgcagtac cgcctcgatc acgttgtgcg
    10021 gggtgatgat ctccttggcg atcagcgacg cgtaggcctc cgacacctcg gctcgccgct
    10081 tgaggaacaa cccctgccat tcgaacaggc cgaagtaccg caccgggtat ctcggatgga
    10141 agatcatctt gagtgccagc cagtcggtga accacccgat gaacaggccg aacagcggca
    10201 tgatgatcgg cagcttgaac aggatccaga cggccatctg cacgatcccg atcaccgcgc
    10261 cgaagtacag accggacctg gcgatgaact ggaactcctt gcggcccgcc tcctggaaaa
    10321 tgcggttgag cagttccttg tccttgacca ggctggtgac caccatgtcc ttgaggtcga
    10381 acacctcgtc cac
//
