LOCUS       NZ_FOET01000019        11402 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces radiopugnans strain CGMCC 4.3519, whole genome shotgun
            sequence.
ACCESSION   NZ_FOET01000019
VERSION     NZ_FOET01000019.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces radiopugnans
  ORGANISM  Streptomyces radiopugnans
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900110735.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 01:56:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,515
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,315
            CDSs (with protein)               :: 5,315
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 41 of 125
            Pseudo Genes (incomplete)         :: 106 of 125
            Pseudo Genes (internal stop)      :: 6 of 125
            Pseudo Genes (multiple problems)  :: 26 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:58:30
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 105318
            Orig. end                         :: 116720
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOET01000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11402
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene-precursor"
                     /region_number="2"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..11402
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11402
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:11065](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..11065
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            complement(172..690)
                     /locus_tag="BMY40_RS24915"
                     /old_locus_tag="SAMN05216481_11993"
     CDS             complement(172..690)
                     /NRPS_PKS="Domain: ACPS (52-117). E-value: 5.7e-13. Score:
                     40.8. Matches aSDomain:
                     nrpspksdomains_BMY40_RS24915_ACPS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012: 4'-phosphopantetheinyl transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /locus_tag="BMY40_RS24915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_11993"
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="WP_177214123.1"
                     /transl_table=11
                     /translation="MPTGSITIGRNPCPKCHSRQHGPPAIHRPATDRWISISHTTGLGL
                     LAVSPFPVGVDVEAVRQVRTDELADTTTTPAERHALLAQPPGTARSTAFLRCWTRKEAV
                     LKATGVGIGSDLNQLETRFWAPGPAHITTNATGTPTTWRVDTLPLPHPWIAALALPHHT
                     PHAVTLQHH"
     aSDomain        complement(340..534)
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BMY40_RS24915_ACPS.1"
                     /evalue="5.70E-13"
                     /label="BMY40_RS24915_ACPS.1"
                     /locus_tag="BMY40_RS24915"
                     /protein_end="117"
                     /protein_start="52"
                     /score="40.8"
                     /tool="antismash"
                     /translation="VGVDVEAVRQVRTDELADTTTTPAERHALLAQPPGTARSTAFLRC
                     WTRKEAVLKATGVGIGSDLN"
     misc_feature    complement(1158..1176)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.98"
                     /tool="antismash"
     misc_feature    1245..1258
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.4"
                     /tool="antismash"
     gene            1454..2155
                     /locus_tag="BMY40_RS24920"
                     /old_locus_tag="SAMN05216481_11994"
     CDS             1454..2155
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981545.1"
                     /locus_tag="BMY40_RS24920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_11994"
                     /product="DNA-binding response regulator"
                     /protein_id="WP_245770307.1"
                     /transl_table=11
                     /translation="MQARALIESTVSLHRRRPAAVSGVMRTDGAVTAETLERLVLRARR
                     SVSVTLTGCAVFAEAVLRSLEQVPSGVVVRVLCTPAAVDVVLNRSRRLSAVRVFRHELN
                     GLVVVDGTLAFLRSASGGEGGGRAAIVTDTTAVSALELLYASAWSRGRRFLGHTELSPR
                     LRTEFTRRILERLRAGHTDAAAARDLQVSLRTYRRHVAEIMRELDASSRFQAGVRAVEL
                     GLLPQERQSAV"
     misc_feature    complement(2171..2186)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.13"
                     /tool="antismash"
     gene            2221..3012
                     /locus_tag="BMY40_RS24925"
                     /old_locus_tag="SAMN05216481_11995"
     CDS             2221..3012
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967405.1"
                     /locus_tag="BMY40_RS24925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_11995"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_093663032.1"
                     /transl_table=11
                     /translation="MCRRPGDEFEYALLQVRELIESTMVIHRDRSRREQLITAVTGGYG
                     KIMRVARELIESAEESVDVLHSRAPGPGEQLEGFGWMENGLLQYARDTVPTRLLVSPEL
                     IEKSLLRRRPDRGGPAAVRVARLPPLEVLTTDGTAALVVAGSAAGRRASLIKAPEVLQV
                     LHTLFESIWCSSVPVGERIAFGDRDRALLIRQILCALRAGVTDEVAARELTVSVRTYRR
                     YVAEIMTLLGASSRFQAGVRAAELGLLPPTTGPGQGGDPGP"
     misc_feature    complement(3032..3045)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.49"
                     /tool="antismash"
     gene            3228..3824
                     /locus_tag="BMY40_RS24930"
                     /old_locus_tag="SAMN05216481_11996"
     CDS             3228..3824
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931934.1"
                     /locus_tag="BMY40_RS24930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_11996"
                     /product="hypothetical protein"
                     /protein_id="WP_093663033.1"
                     /transl_table=11
                     /translation="MTQRPVGLVFPGPGRPRQAVGGFRRGPLSRDATAGISQSTGEDLP
                     EPPLRTPAGRLRRVGLARLSVRAGAVTAPCGAVRCGATGRPVAACAGRRPGGYTALAAG
                     ALAVGPRDTVLALRRRPGVTNAAARPYGGGRTGAAARRLTSPTGREQSVRAHTGPLGCR
                     RPVAPEPDGGSRAGLIRRTAQDIEAVSGDGPGALD"
     gene            3996..4781
                     /locus_tag="BMY40_RS24935"
                     /old_locus_tag="SAMN05216481_11997"
     CDS             3996..4781
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967403.1"
                     /locus_tag="BMY40_RS24935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_11997"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_093663034.1"
                     /transl_table=11
                     /translation="MTTAAREWVSGVGVLDKASVLLEIVEQAPAPLSELVSRSGLARPT
                     VYRIAVALERLGLLTRDCRGRFVLGPRFGALAVQARYDRLVRAAAPVLAELHAATGLEA
                     RIVRRRGRVQVCVATSADGVAGPVGADKAPVGTARPVTAGAVALVLLAWEEPEEIYHGL
                     RGARFTAGQLSQVRRRGWARGADVMVPGEVAYAVPVRGGDGRVVAALSVSGTPGRMGLV
                     PSRRLGAAMFDSAAELGDALQRARTAPAAPRAPAAPRGR"
     gene            complement(4856..6877)
                     /locus_tag="BMY40_RS24940"
                     /old_locus_tag="SAMN05216481_11998"
     CDS             complement(4856..6877)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016824057.1"
                     /locus_tag="BMY40_RS24940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_11998"
                     /product="AarF/UbiB family protein"
                     /protein_id="WP_245770308.1"
                     /transl_table=11
                     /translation="MKPVAVLLIAAAGALLGPMIMAVVARRLLGLRIGRVRALLTGCVG
                     LLAAGVVGTPMGSAASRAPLVTVQLGAALIAAMIFLALSEAVVPSGSVRGVVRWPGAVR
                     RRLARSRRYTQLSRIAVRHGLRRFLTGRPRRAGKSPQEQAELARSLRLALEEAGATFVK
                     LGQVLSSRYDLLPDVFVDELSSLQHEVAPEPWPDIERVLAEELGASPVEVFAEFDPEPL
                     AAGSMAQVHRARLRGGQAVAVKVQRPGARQIVERDLDILFRISDMLERHTQWGRTVGSR
                     ELVQGFADSLHEELDFRTEARNTVAVAANAAPSGDTGPAGGAGGGAQQSTEDQSFDIAP
                     RVRFPRVHQELCSERVLVVEWLDGVTLNRADAVIDARGLDRGELARDLLGCLLGQIMSE
                     GVFHADPHPGNILVLEDGRLGLVDFGSLGRIDQMLRSSLRNLLMAIDRGDPLAMSDALL
                     ELVERPEEIDEERFKRALGRFTARHLTSGAPPSHEMFTDLFVIVAQYGLTVPPEVAAAF
                     RALTTVEGSLNRLMPGFDIVEEARSFASRELTRRLSPQHLSRSLADEAAAVLPMLLRLP
                     RRAERISSALEHGRLSVNVRLLADERDRRFIRGIVRDVLLAFLGGLTGVLGMLLLAAEG
                     GPRISDSLRLFDVFGYNLLLVSTVLVLRVLFTITKPQR"
     gene            complement(6996..9140)
                     /locus_tag="BMY40_RS24945"
                     /old_locus_tag="SAMN05216481_11999"
     CDS             complement(6996..9140)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1035: MMPL domain-containing transport protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931928.1"
                     /locus_tag="BMY40_RS24945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_11999"
                     /product="MMPL family transporter"
                     /protein_id="WP_093663035.1"
                     /transl_table=11
                     /translation="MFGRVGRVAVRHPWLTILVWVIAAMGTAALAPPLKPVSDQAEFLP
                     SHYESVRAGELQGRAFPQQEQPASVMVFRRDDGGKLTGEDLADVQKVAAGLEKAGLDKI
                     KKVETSAQAVSPNREIALAGIVATTKDPYNADLLDSIKQMREEAKPLLKGTSLHMGITG
                     ASATTLDTLESSGDTDAMIMMATLVLIVVLLAAIFRSPLIAILPVLVITLVFVVATGLI
                     STAAEFGGFQADASIGSILIVVLFGVGTDYILFLLFRYREFLRLGQEPKEAMGEAVARV
                     GETIASAAGAVIAAFLALLLSSLGMLRAMGPALAISVAVTLLAALTLVPAVFSLLGTKA
                     FWPSKAWRREPRHGLAARTASLTARRPALVAAASGGLLAVLAAGVLNLTTVFDNAGSLP
                     KNLESVQAAETLERGFSAGQTDPTQVFLEAEDGGKLERSQVLAYERELSAAGLGQVTPA
                     VFSPEGSVARFGVVLEHKPDSTQAIDVVSGPLRDAAHQAAPEGTRVLVGGTSAVLADIQ
                     HATHRDYSVVFPAAGIAIMVILGLLLRSVVAPWYLMLAVGLGFAATLGSTVWLFQNLMG
                     EAGLPFTLPVIVYLFVVAIGTDYNILVVARLREEVKRGNAPAQAVRQAITHSASTIGTA
                     AAILAGTFGVLLLAQNSMLQQMGFAVAFGILLTAFVMALLLVPAVTTLLGHRTWWPGRV
                     TPDAEPEEAPAREPALARHG"
     misc_feature    9725..9739
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.1"
                     /tool="antismash"
     gene            10001..11065
                     /locus_tag="BMY40_RS24950"
                     /old_locus_tag="SAMN05216481_119100"
     CDS             10001..11065
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931926.1"
                     /locus_tag="BMY40_RS24950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_119100"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_245770309.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 8.3e-50, bitscore:
                     162.1, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAKQLYEAPLPASPDGRLSYSSVLLSRLGPNRPDFEARLGRALA
                     AAERRLQACSRDASDPRAAELTGHLTATGGKRLRTLLALLAAEFGDPSREGVVQAAVVA
                     ELVHVASLYHDDVVDRAATRHGASTANVLWGNRMAVLGGDWLLARAARLAADLVPGAFS
                     LNADTANRLIAGQLRELAGPAPGEDPVEHYLQVTAGKTAALLAMALGVGALQAGAPEAV
                     ADILIEYGEQLGIAFQIADDLLDLSSPDAFTGKERGQDLLAGVPSLPVLLAREDTDPRD
                     AELRELIEAGSAAGADWHLRVLELFPSSRATARAEAIMHQRLARAKGALESLPPLPARR
                     TLDALCDFVAFRTG"
     gene            11085..>11402
                     /locus_tag="BMY40_RS24955"
                     /old_locus_tag="SAMN05216481_119101"
     CDS             11085..>11402
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016828557.1"
                     /locus_tag="BMY40_RS24955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_119101"
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="WP_281250800.1"
                     /transl_table=11
                     /translation="MPQETAPRTERAATHHRAAELARRRREVLQGDPKATEAQHAKGKL
                     TARERIELLLDEGSFQEVEPLRRHRATGFGLEDRRPYTDGVVTGWGTVEGRTVFVYAHD
                     FR"
ORIGIN
        1 ccttcgacac caccttcgac ggccgactct ccgcagcctg ccaccaaccc cgaccgcccc
       61 cgtcgcaccg ctcgatcgac agtcccggga acgacctccg acgcgcaggt gatgttggat
      121 ctttcttcgg gtttgtctgt gtgtgggggc ggttgggggt gtggtggttg ttcagtggtg
      181 ttggagggtg acggcgtggg gggtgtggtg ggggagggcg agggccgcga tccaggggtg
      241 ggggaggggg agggtgtcca cgcgccaggt ggtgggggtg ccggtggcgt tggtggtgat
      301 gtgggcgggg ccgggggccc agaagcgggt ttccagttgg ttcaggtcgg agccgatgcc
      361 gactccggtg gctttcagga cggcttcctt gcgggtccag cagcggagga atgccgtgct
      421 gcgggcggtg cccggtggtt gggcgagcag tgcgtgccgt tcggcggggg tggtggtggt
      481 gtcggcgagt tcgtcggtgc gtacctggcg gaccgcctcg acatcgacgc cgacggggaa
      541 gggggagacg gccagcaggc cgaggccggt ggtgtgggag atgctgatcc accggtcggt
      601 ggcggggcgg tggatcgcgg gtgggccgtg ctgccggctg tggcacttgg ggcaagggtt
      661 ccgaccaatt gttatcgagc cggtcggcac ttgaagaaat tcggacaata tgcgacgcac
      721 tgctgctcgg caggtaatga actcgttggc cgcggcggcg gatttcattt ggtctgcacg
      781 actgcgttct gcggtgtcga gaatggcgat atcggtcctg cttgatcctt tgggtgacac
      841 tttccaccac cacacgtgag tggtggtggc gtggcgattg ggcgtcaaag gggttgcggg
      901 gccgggaggc cgtatgtgtc ctgtaggcac cgggtcaccg taggttccca cgttagaaag
      961 gggcgggggt cggccgtcgg gtgtccgcta gcggacacct atgtcacatt cggtgacgcc
     1021 ctgtgcgcat agagtcgcgc atatggaatc ggaacaactg attccgttac ctggcttgca
     1081 tcttgctgac agcgcgggag ggggaaaggt cgaatattgg tgaagagttg tggttctctg
     1141 catttgattt cggccatact gcgaggcgat ctcactgact gagacgtaac gtgcgggtca
     1201 ctgtccgggg ggcaagtcgt catgcgttcg gtgcggagta tttgcttttt caataagcgc
     1261 cgggcggatc gacggtggcc gcctgagtcg cccggctgag gtaccggggc tgagacgcgc
     1321 gaaagggcac gcaggctttg aacgatcatg gggggaatca cacgtggcag gtactgcatt
     1381 cgatcttcca ggtgtcggcg gactggaggg gagcgctgcc cagagccacg ccgctcacct
     1441 ggaacagacg ctgttgcagg ctcgtgcact gatcgagtcc acggtctccc tgcaccgccg
     1501 gcgtccggcc gcggtgtcgg gggtgatgcg gaccgacggc gcggtgaccg ccgagaccct
     1561 ggagcggctc gtgctgcggg cccggcgctc ggtgagcgtg acgctcaccg gctgcgccgt
     1621 gttcgccgag gctgtcctga ggagcctgga acaggtgccg agcggagtgg tcgtgcgcgt
     1681 cctgtgcact cccgccgccg tcgacgtggt gctcaaccgg tcccgccgcc tgtcggcggt
     1741 ccgggtgttc cggcacgagc tgaacggact ggtggtggtc gacggcacgc tggcgttctt
     1801 gcggtccgcc tccggcgggg aggggggcgg ccgggcggcg atcgtcaccg acaccacggc
     1861 ggtcagcgct ctggagctgc tctacgcgag cgcgtggtcg cgcgggcgca ggttcctcgg
     1921 ccacacggaa ctgagccccc ggctgcgcac cgagttcacg cgccgcatcc tggagcggct
     1981 gcgggccggg cacaccgacg ccgccgccgc gcgtgacctg caggtgtcgc tgcgtaccta
     2041 ccggcggcat gtcgccgaga tcatgcggga actcgacgcg tcctcccgtt tccaggccgg
     2101 cgtgcgagcg gtcgagttgg ggttgctgcc gcaggagcgg caatccgccg tgtgacgcgg
     2161 tgcggccggt gtgaagtaac cgtgagaacg aactgccgac ggtgattgtg gggtcacagc
     2221 gtgtgcagac gtcccgggga cgagttcgag tacgcccttc tacaggtcag ggagctgatc
     2281 gagtcgacca tggtcatcca tcgcgaccgc agccggcggg agcagctcat cacggcggtc
     2341 accgggggct acgggaaaat catgcgtgtc gcccgtgagc tgatcgagag tgccgaggag
     2401 agcgtcgatg tcctgcactc ccgggcaccg ggccccggcg agcagctgga gggcttcgga
     2461 tggatggaga acggtctgtt gcagtacgcg cgggacacgg tcccgacacg gctgctggtc
     2521 agtccggagc tgatcgagaa gtccctgctg cgccggcggc cggaccgggg cgggccggcg
     2581 gcggtccggg tggcccggtt gccgccgttg gaggtgttga ccacggatgg caccgccgcc
     2641 ctggtggtgg ccgggtccgc ggccggccgc cgggcctccc tcatcaaggc ccccgaggtg
     2701 ctgcaggtcc tgcacaccct gttcgagagc atctggtgca gctcggtgcc cgtcggcgag
     2761 cgcatcgcct tcggcgaccg tgacagggca ctgctgatcc ggcagatcct ctgcgccctg
     2821 cgggccgggg tcaccgacga ggtcgccgcc cgcgagctga cggtctccgt gcggacctac
     2881 cggcgctacg tcgcggagat catgacgctg ctcggggcca gctcacgctt ccaggcgggg
     2941 gtccgggccg cggagctggg gctgctcccg cccacgacgg ggcccgggca gggcggtgat
     3001 cccggcccgt gaagcggcct ggcagcttca tgaaacgggc gttgcccctc tcccgcgccg
     3061 cacagttgga tgaagccggg gacggagttc cggttcgagg gctttcccgt ccccggcggc
     3121 ccgccacccc ccccgcaggc cgccggtacg cggggacccg cacgcctcgg ccctggcacc
     3181 ggcgggtccc cgttgccccg gtgcatttcc gtgcggaagg cggatccatg actcagcggc
     3241 cggtcggcct ggtgtttccc ggtccgggga ggccgaggca ggcggtgggc gggttccggc
     3301 gcggccccct gtcccgggat gcgaccgcgg ggatctccca gtccaccggg gaggacctcc
     3361 ccgaaccgcc gctccgcacc ccggccggac ggctccggcg cgtcggcctg gcgcggctct
     3421 cggtgcgcgc cggcgcggtg accgcccctt gcggggcggt gcggtgcggg gcgaccggca
     3481 ggcccgtggc cgcctgcgcg ggccgccgcc cgggcgggta caccgccctc gccgccgggg
     3541 cgctcgccgt cgggccgcgg gacacggtgt tggccctccg gcgccggccg ggggtgacca
     3601 acgccgccgc gcggccgtac ggcggcggcc ggaccggggc cgcagcccgc cggctcacct
     3661 cgccgacggg ccgggagcag agcgtgcgcg cccacaccgg ccccctcggc tgccgccgcc
     3721 cggtcgcacc ggagccggac ggcggcagcc gggcgggact gatccgccgg accgcccagg
     3781 acatcgaagc ggtgtccggg gacggccccg gcgccctgga ctgacgacca tcccgcgcgg
     3841 ggaggagagc ggaacaccat gccacctggc accgaccaca tccggctgtg tcctcacgcc
     3901 gtttcggcgc cgctgcgcag gcggctgccg ggccgccggg cggtggcgtg cgtcgacggc
     3961 ggcggccttt gccgcagacc tgcggcggtc cggcgatgac cacggcggcc cgggagtggg
     4021 tcagcggggt cggcgtgctc gacaaggcgt ccgtactgct ggagatcgtc gagcaggccc
     4081 cggcgccgtt gagcgaactg gtctcccgca gcggcctcgc ccgtcccacc gtgtaccgga
     4141 tcgcggtggc gctggaacgg ctcggactgc tcacccgcga ctgccgcgga cggttcgtcc
     4201 tcggccccag gttcggcgcc ctggcggtgc aggcgcgcta cgaccggctg gtccgggcgg
     4261 cggctccggt gctggccgaa ctgcacgccg cgaccgggct ggaagcacgc atcgtgcggc
     4321 gccgcgggag ggtgcaggtc tgcgtcgcga cctccgcgga cggtgtggcc ggacccgtcg
     4381 gcgccgataa ggcgccggtg ggcacggcca gacccgtcac cgctggtgcg gtcgccctgg
     4441 tgctgctcgc ctgggaggag cccgaggaga tctaccacgg gctgcgcggc gcccggttca
     4501 ccgccggcca gctctcccag gtgcgtcgcc gcggctgggc caggggagcc gacgtcatgg
     4561 tgcccggcga ggtcgcgtac gccgtgcccg tgcgcggcgg ggacggccgg gtggtcgccg
     4621 cgctgtcagt gtcgggtacg ccgggccgga tgggcctggt ccccagccgg cggctcggcg
     4681 cggccatgtt cgactcggct gccgaactgg gggacgcgct gcagcgggcc cgcaccgcac
     4741 cggccgcccc gcgcgcaccg gccgcgccga gggggcgatg acggcggtgg ccacccgccg
     4801 cgaccgccgc gggcagggcc cgccggtgtc cggccgcgac cggacaccgg cgccctcacc
     4861 gctgcggttt ggtgatcgtg aagagcaccc gcagcaccag caccgtactg accagcagca
     4921 ggttgtaacc gaagacgtcg aacagcctca gtgagtcgga gatgcgaggg ccaccctcgg
     4981 cggccagcag cagcatgccc aggaccccgg tcagtccgcc gaggaaggcg agcagcacgt
     5041 cacggacgat gccccgtatg aagcgtcggt cccgctcgtc cgcgagcagc cgtacgttga
     5101 cgctgagcct gccgtgctcg agcgcgctgc tgatgcgctc cgcccggcgc ggcagccgca
     5161 gcagcatcgg cagcacggcg gcggcctcgt ccgccaggga ccggctcagg tgctgggggg
     5221 agagtctgcg ggtgagctcg cgggaggcga aggaacgtgc ctcctcgacg atgtcgaatc
     5281 ccggcatgag ccggttcaag gagccctcca cggtggtcag cgcgcggaag gccgcggcca
     5341 cctcgggcgg cacggtgagt ccgtactggg ccacgatcac gaacaggtcg gtgaacatct
     5401 cgtggctcgg gggagcgccc gaggtcaggt ggcgggccgt gaagcggccc agcgcgcgct
     5461 tgaagcgctc ctcgtcgatc tcctcggggc gctcgaccag ttccagcagg gcgtcggaca
     5521 tcgccagcgg atcgccgcgg tcgatcgcca tgagcaggtt gcgcaggctg gagcggagca
     5581 tctggtcgat ccggcccaga gagccgaagt ccaccaggcc cagcctgccg tcctccagga
     5641 ccaggatgtt cccgggatgg gggtcggcgt ggaagacgcc ctcggacatg atctgcccca
     5701 gcaggcagcc gagcaggtca cgggcgagtt ccccgcggtc caggccgcgc gcgtcgatga
     5761 cggcgtcggc ccggttcagg gtgaccccgt cgagccactc gacgaccagc acccgctcgc
     5821 tgcacagttc ctggtggacc cgggggaacc gcacgcgcgg cgcgatgtcg aagctctggt
     5881 cctccgtgct ctgctgcgct ccgccgccgg ccccgcccgc gggcccggtg tcccccgacg
     5941 gggcggcgtt cgcggccacg gccacggtgt tgcgggcctc ggtgcggaag tccagttcct
     6001 cgtggagcga atcggcgaac ccctgcacca gttcacggga gccgacggtc cggccccact
     6061 gggtgtgccg ctccagcatg tcgctgatcc ggaagaggat gtccaggtcg cgctccacga
     6121 tctgccgggc gcccggccgc tgcaccttga cggccaccgc ctggccgccg cgcagccggg
     6181 cccggtgcac ctgggccatg gagccggctg ccagcggttc ggggtcgaac tccgcgaaca
     6241 cctccaccgg cgaggctccc agctcctcgg ccagcacccg ctcgatgtcg ggccagggtt
     6301 caggggcgac ctcgtgctgc aacgagctca gctcgtcgac gaacacgtcg ggcagcaggt
     6361 cgtaacggga ggagagcacc tggcccagct tgacgaaggt ggctcccgcc tcctccagcg
     6421 ccagccgcag ggaccgggcc agttccgcct gctcctgcgg gctcttgccg gcgcgtcggg
     6481 gccggccggt caggaagcgc cgcagcccgt gccgcacggc gattctgctg agttgcgtgt
     6541 agcgccggct gcgcgccagc ctgcggcgca ccgcaccggg ccagcggacc acgccgcgca
     6601 ccgacccgct ggggaccacg gcctcggaga gcgccaggaa gatcatggcg gcgatcagag
     6661 ccgcgccgag ctgcacggtc accagcgggg cgcggctcgc cgcggaaccc atcggggtgc
     6721 cgaccactcc tgcggccagc agtcccacgc acccggtgag aagggcgcgg accctgccga
     6781 ttcgcagtcc gagcagtctg cgggccacga cagccatgat catcgggccg agcagagcgc
     6841 cggccgcggc tatcaggaga acggcgactg gtttcacccc gctgatcgta ggagctgttc
     6901 cgtgccgtcg gacgacacga tcacaagatg cccggcgccc ctccgcgaag aggagcgccg
     6961 cccggccccc gggggccggg cggcaatccc gtccgtcacc cgtgccgggc gagggcgggc
     7021 tcccgcgccg gagcctcctc cggctcggcg tccggcgtga cgcggccggg ccaccacgtc
     7081 cggtgaccga gcagggtcgt cacggcgggc accagcagca gggccatcac gaaggcggtg
     7141 agcaggatgc cgaaggccac ggcgaagccc atctgctgaa gcatggagtt ctgggccagc
     7201 agcaggacgc cgaaggtgcc cgcgaggatg gcggccgccg tgccgatggt cgacgccgag
     7261 tgggtgatcg cctggcggac ggcctgcgcc ggggcgttac cgcgcttgac ctcctcgcgc
     7321 agccgggcca ccaccaggat gttgtagtcg gtgccgatcg cgacgacgaa caggtagacg
     7381 atcacgggca gcgtgaaggg cagaccggcc tcgcccatga ggttctggaa gagccagacg
     7441 gtggatccca gcgtcgcggc gaagcccagg ccgaccgcga gcatcaggta ccagggggcg
     7501 acgacgctgc gcagcagcag cccgaggatc accatgatcg cgattccggc ggccgggaag
     7561 accacggagt agtcgcggtg ggtggcgtgc tgaatgtcgg cgagcaccgc cgaggtgccg
     7621 ccgaccagga ctcgggtgcc ctccggcgcg gcctggtggg cggcgtcgcg cagcggtccg
     7681 gagaccacgt cgatggcctg ggtgctgtcc ggcttgtgct cgagcaccac accgaaccgg
     7741 gcgacgctgc cctcggggct gaagaccgcg ggtgtgacct gcccgagacc ggccgcggac
     7801 agctccctct cgtaggcgag gacctggctc cgctccagct tgccgccgtc ctccgcctcc
     7861 aggaacacct gggtcgggtc ggtctggccg gccgagaagc cgcgctcgag cgtctccgcc
     7921 gcctgtaccg actccaggtt cttcggcagc gaaccggcgt tgtcgaagac tgtggtgagg
     7981 ttgaggacgc ccgcggccag cacggccagc agaccgccgg aggcggcggc gacgagagcg
     8041 gggcggcgcg cggtgaggga ggcggtccgg gcggcgagcc cgtgccgcgg ttccctgcgc
     8101 catgccttgg acggccagaa cgccttggtg cccagcagcg agaagaccgc gggcaccaga
     8161 gtcagtgcgg ccagcagggt gacggcgacc gagatcgcca gggcgggccc catggcccgc
     8221 agcatgccca gggaggacag cagcagggcg aggaaggcgg cgatcacggc gccggcggcc
     8281 gaggcgatgg tctccccgac ccgggcgacg gcctcgccca tggcctcctt cggctcctgc
     8341 cccaggcgca ggaactcccg gtagcggaag agcaggaaca ggatgtagtc ggtgccgaca
     8401 ccgaacagca ccacgatcag gatggacccg atcgaggcgt ccgcctggaa cccgccgaac
     8461 tcggcggcgg tggagatcag cccggtggcc accacgaaga ccagggtgat gaccaggacc
     8521 ggcaggatcg cgatcagcgg gctgcggaag atcgcggcca gcaacacgac gatcagcacc
     8581 agggtcgcca tcatgatcat ggcgtcggtg tcacccgagg actccagggt gtccagcgtg
     8641 gtcgccgagg ctccggtgat ccccatgtgc agggaggtgc ccttcaaaag gggcttggcc
     8701 tcctcccgca tctgcttgat cgaatcaagc agatcggcat tgtaggggtc cttggtggtc
     8761 gcgacgatgc ccgccagggc gatctcgcgg ttgggggaga cggcctgcgc gctggtctcc
     8821 accttcttga tcttgtccag tccagccttc tccagaccgg cggcgacctt ctgcacgtcc
     8881 gccaggtcct ccccggtcag cttgccgccg tcgtcacggc ggaagaccat gacggaggca
     8941 ggctgctcct gctgcgggaa agcacgtccc tggagttccc cggcccgcac cgactcgtag
     9001 tgcgagggga ggaattcggc ctggtcgctg accggtttca ggggcggagc cagagctgcc
     9061 gtgcccatcg ccgcgatcac ccacaccaat atggtcagcc aaggatgtct gacggcgacc
     9121 cgtccgaccc gtccgaacat ggggggaggc tccttcgaaa gggggttcat aaggcggacc
     9181 gattgtcttc cgcgtgcgtc cgtcgccgtc ggctcccagc ggcatctggc atcaagttgc
     9241 cgttcgccgc cggcaggccg ccccgcccct cccgccggag cgcggccggc gggagcgccc
     9301 ccgggaagcg accgcggcgg ctgccccggc cgaaccctca ccgcccgcgg gccgatccgg
     9361 ccggcgcctg ccgaattccc gcaaaccgtg ccgccggaac gaagtcggcg gtaaatcatc
     9421 gggatctcct cggccgccgc gcttccacgg catggtgacg gaaggtgaaa aatcccgccc
     9481 gaagcttccg gatcaaaagg aaaaccggtg ccgggcaaac agttcacggg gctcgccgtt
     9541 cgtttccctc ggcgtgcccg gtgccgccac cggattccgg agagccccgc ggcagcggac
     9601 ggtccccggc gtgcgcgcac ctccggtccg gccccggcca agcgagccgg tgccgcgtcg
     9661 ccgccacgcc cgcgcgaccc gggggacggg cgtgcgcgcc cggccccgga gacgtccggc
     9721 gccttggggg cgctccccgg tcggacccgg cggcacgcgg ccggggcgag gactgcgggc
     9781 cggacggccg gacgcgggaa ttaccgccgc ccatgtcgtt ctccgtttca ccgttccccc
     9841 cgattctccg tttccggact tcgaacctcg cggcatcggg gcggacggtg gcaccttcat
     9901 gccaggccgg ttgtcgctcg tttggcggac tgcgaggatc acggccgaag gcaaccgacc
     9961 gcgatttccg tcccgcaggc ccgacaggat gagtttcctc gtgaccgcaa agcagttgta
    10021 cgaggctccg ctgcccgctt caccggacgg ccgtctgtcg tattcctccg tgctcctgag
    10081 ccggctcggt ccgaaccggc cggacttcga ggcccgtctg ggccgggcgc tggccgctgc
    10141 cgaaaggcgg ctccaggcct gctcccgcga cgcctccgat cccagggcgg cggaactcac
    10201 cgggcatctg acggccacgg gcggcaagcg gctgcgaacg ctcctggcgc tgctcgccgc
    10261 ggagttcggg gacccgtcgc gggagggcgt cgtccaggcg gccgtcgtcg ccgaactcgt
    10321 ccacgtcgcc tcgctctacc acgacgacgt ggtggaccgg gccgccaccc gccacggtgc
    10381 ctccaccgcc aacgtcctgt ggggcaaccg gatggccgtc ctgggcgggg actggctgct
    10441 ggccagggcc gcccggctcg ccgccgacct ggtgcccggg gcgttcagcc tcaacgccga
    10501 cacggcgaac aggctgatcg cgggccaact gcgcgaactc gccgggcccg cgccggggga
    10561 ggaccccgtc gagcactacc tccaggtgac ggccggcaag accgccgcgc tgctggccat
    10621 ggccctgggg gtcggcgccc tccaggccgg cgcgcccgaa gcggtcgcag acatcctcat
    10681 cgagtacggc gaacagctcg gcatcgcgtt ccagatcgcc gacgacctgc tggacctctc
    10741 ctcccccgat gccttcaccg gcaaggagcg gggccaggac ctgctcgccg gagtccccag
    10801 cctcccggtg ctgctcgccc gggaggacac cgacccgcgc gacgccgaac tgcgcgaact
    10861 gatcgaggcc ggatccgctg ccggagccga ctggcacctg cgagtcctcg aactcttccc
    10921 ctcctcgcgg gccaccgccc gtgccgaggc gatcatgcac cagcggctgg ccagggcgaa
    10981 gggggcgctt gagtccctgc caccgctgcc tgcgcgccgc acactcgacg ccctctgcga
    11041 cttcgtggcc ttccgtaccg gctgagagga acagggagtt ggccatgccg caggagacag
    11101 cccctcgaac cgaacgggca gccacgcacc acagggccgc ggaactcgcc cgcaggcgcc
    11161 gggaggtgct ccagggcgac ccgaaggcca ccgaggctca gcacgccaag ggcaagctga
    11221 ccgcgcgtga gcggatcgaa ctgctgctgg acgaggggtc gttccaggag gtggagccgc
    11281 tgcggcggca ccgcgccacc ggtttcggcc tggaggaccg caggccgtac accgacgggg
    11341 tggtcaccgg ctgggggacg gtcgagggcc gtaccgtctt cgtctacgcc cacgacttcc
    11401 gc
//
