LOCUS       NZ_FOET01000008        11447 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces radiopugnans strain CGMCC 4.3519, whole genome shotgun
            sequence.
ACCESSION   NZ_FOET01000008
VERSION     NZ_FOET01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces radiopugnans
  ORGANISM  Streptomyces radiopugnans
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900110735.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 01:56:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,515
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,315
            CDSs (with protein)               :: 5,315
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 41 of 125
            Pseudo Genes (incomplete)         :: 106 of 125
            Pseudo Genes (internal stop)      :: 6 of 125
            Pseudo Genes (multiple problems)  :: 26 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:58:30
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 130943
            Orig. end                         :: 142390
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOET01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11447
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11447
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11447
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6447](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6447
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            853..1992
                     /locus_tag="BMY40_RS16360"
                     /old_locus_tag="SAMN05216481_108125"
     CDS             853..1992
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /locus_tag="BMY40_RS16360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_108125"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_218158304.1"
                     /transl_table=11
                     /translation="MTVSVRRTARPLTALALVAAAAAGCAGPGGDTTRAGASPAAPSSP
                     AAPEEAGEGSGEEPEEKTSGKGDFSASPRSSVSRPEGDDRDASSSGRYATLPASISGRV
                     PVFRHGPRAEAPRDARGEEKAEEDGENREKREKSENAEKTEKTEKAEKAEKAEKSGDKG
                     DKVVALTFDAALSAEDRERAEEDGKRHDNPGLIAALRREKVPATVFMSGLWAQTYKHQA
                     RSIGRDDLFEVGNLSYSHRAFTRDCRDLPALAPGKQLADVRKGQKAIRAAGVKNLTPYF
                     RFPGGCFDDRSRRAVAPARVTVVAGDVVAPDAEEKDPGKVAERVLKDIRPGSVVVLHCD
                     REKAPATEKAVRRIVPELRERGYRIVHVSDMIASAMGRD"
     gene            complement(2027..2245)
                     /locus_tag="BMY40_RS16365"
                     /old_locus_tag="SAMN05216481_108126"
     CDS             complement(2027..2245)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006345811.1"
                     /locus_tag="BMY40_RS16365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_108126"
                     /product="hypothetical protein"
                     /protein_id="WP_093660180.1"
                     /transl_table=11
                     /translation="MEFVSGWTPPETAAARDGGEERAEGTLDGAGREERAVCVQCGEPT
                     EYPASTRGATLCPVCEWQEAERVACSC"
     gene            2335..3111
                     /locus_tag="BMY40_RS16370"
                     /old_locus_tag="SAMN05216481_108127"
     CDS             2335..3111
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100702.1"
                     /locus_tag="BMY40_RS16370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_108127"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_093660182.1"
                     /transl_table=11
                     /translation="MSTSDSSSDAFSEPAATRGATARDAAPQYVLPLVVRIERSAPPGP
                     PARTDALRTAARAVLVMLSDERATDPGGPWHQAVRDWQDGRIRKVVRRARGAEWRRACA
                     LPGVTVTGDGGHPAEVRVFPPVPLDGWPKDLARLQVSGTELEDPVPPVAPEPGTPVLWL
                     NPELEMSAGKAMAQAGHGAQLAWWALDGAARAAWRAAGFPLAVRTAPRGRWERLTESGL
                     PVVRDAGFTEIAPGSATVVAEHPALPARPVRPAPRG"
     gene            complement(3116..4837)
                     /locus_tag="BMY40_RS16375"
                     /old_locus_tag="SAMN05216481_108128"
     CDS             complement(3116..4837)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358873.1"
                     /locus_tag="BMY40_RS16375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_108128"
                     /product="BCCT family transporter"
                     /protein_id="WP_177214029.1"
                     /transl_table=11
                     /translation="MLEKLQRLLGLRTDPVIFFTSAGLTVLFVVAAISLTEQVDRIFAT
                     ASDWLLTNLGWFYILGVTTFLVYLLWIAVSRYGHIRLGAQDDRPEYSRRTWFAMLFAAG
                     IGTILMFWGVAEPISHFAEPPQQGVEPQSTEAARMAMAYSLYHFGLHTWSIFCLPSLTF
                     AYFVYVRGMPFRVSSVLYPLLGDRVYGTAGRIVDIAAVMGTLFGVAVSIGLGTMQINSG
                     LARLFGLPENSLVQVLLITVITAIATTSVVYGLDKGIRRLSNINIVMAIGLMLFVFATG
                     ATLFLAKGIIENVGVYLSSLVSLAFWNDTFADTGWQGTWTVFYWAWTITWSPFVGIFIA
                     RISKGRTIREFVLGVLALPTAFTIVWFSVFGMSAIDIELNGPGGLVREVVEEGDIPGAL
                     FTFLENYPLTTLASALSVLIVVIFFTTSSDSASLVVDMLSAGQREEGGPTRQRVFWAIT
                     EGVVAGTLIAATGKSGLDALAEVITVLGLPFFVLAFLMMYSLVRALRTDFPELTGPPAA
                     LRRRMERERRTREWGAGKRDGENAEGIEGTGGAGRAGRAGGETSEGAGGGASEGGR"
     gene            5001..6447
                     /locus_tag="BMY40_RS16380"
                     /pseudo=""
     CDS             5001..6447
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432057.1"
                     /locus_tag="BMY40_RS16380"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 3.3e-87, bitscore: 285.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPHGEEPSRGAGVARLGTGIGWRPKIADAIESLPDVGWVEVVA
                     ENVCPDHLPPALVRLRERGTTVVPHGVGLGLGGAQRPDPARLAALARRAEALEAPLVTE
                     HIAFVRAGGPGVEGPGVEGPGVEGPGVEGPGVEGPVMEAGHLLPVPRTRDALDVLCENV
                     TIAQDALPVPLALENVAALFTWPGEELTEAEFLTELVDRTGVGLLIDVANLHTNRVNRG
                     EDPLAVLDGLPLDALAYVHVAGGVERDGVWHDSHAHPVPEPVLELLAELCARTDPPGVL
                     LERDDDFPAAEELAAELAAVRGVFEGTARGAGGTRV"
     gene            complement(6516..6728)
                     /locus_tag="BMY40_RS16385"
                     /old_locus_tag="SAMN05216481_108131"
     CDS             complement(6516..6728)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175843.1"
                     /locus_tag="BMY40_RS16385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_108131"
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_093660184.1"
                     /transl_table=11
                     /translation="MSVEQVTDNAGELYWFKSSHSGSDGGDCVEVAAGPDAVYVRDSKA
                     AGDGPIVRVGQGGWAAFLVLAAEQS"
     gene            complement(6725..7573)
                     /locus_tag="BMY40_RS16390"
                     /old_locus_tag="SAMN05216481_108132"
     CDS             complement(6725..7573)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576590.1"
                     /locus_tag="BMY40_RS16390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_108132"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_093660354.1"
                     /transl_table=11
                     /translation="MRDTRNGRAVRVKEENGDPPGVWSAYGRLLQHLRKQAGLSQQKLG
                     DAIGYSLEQVASVEQGRRPAKAAFTIAADRALGARGVLEVLQDDVERAKLPRFFRNFAL
                     IESEALSRFSYDPLLVPGLLQTEGYARAVFGGHCPPLSEETIDQHTEARLSRQKLLTRV
                     PMAELSFIISEEALRDPVGGPEVMREQWRRLLEVNALRNVEVQIMPAGRGFHPGKNGPF
                     VILETKEHRDIGYFESQGVGCVVSDVSEVRAFSMRYGKLRSQALNVEESARLIERLVGE
                     T"
     gene            7990..9168
                     /locus_tag="BMY40_RS16395"
                     /old_locus_tag="SAMN05216481_108133"
     CDS             7990..9168
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BMY40_RS16395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05216481_108133"
                     /product="hypothetical protein"
                     /protein_id="WP_093660186.1"
                     /transl_table=11
                     /translation="MRFTLRRKQPLEEESGACGKGLGDLGEGIGRISREIAEIRTALAE
                     AAEKPPSQAMSGELHRLRRTASDGIRILQEENQTQLRRQEEILGRLGELRLEITGGRTG
                     AHPPPAPGSPGADEPGGADGTGEAGDGTAGTAGAQSLAHSSAPSDEAPETQDSRRQEER
                     MEQEQDGVPEPPDGAAPSRETEETPEERVRKVKEAAEQALRNRSEDSRGRTDRTATSPV
                     ADDRRLHGVHADGTDGQRPGRQDGGRAAPRDPLAEHAELLLNAAGVSRAELVCHRDTWE
                     FLLVQAVRHPRFRVPGEVKDIGDGKVGTHLSGPSLIAALISLWKTREEPSEEVDADWAM
                     AAVLYDRIRDRLTEAGPGDGRRTVRIVLDDGDGPEETAVAAETSAEEEPEAA"
     gene            complement(9232..9441)
                     /locus_tag="BMY40_RS16400"
                     /old_locus_tag="SAMN05216481_108134"
     CDS             complement(9232..9441)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469277.1"
                     /locus_tag="BMY40_RS16400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_108134"
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_093660188.1"
                     /transl_table=11
                     /translation="MSIERQGGDAPGELSWSKSSHSGPNGGDCVEVAAGADAVYVRDSK
                     TAGGPVLWIGRDGWAAFVALAAGR"
     gene            complement(9438..10286)
                     /locus_tag="BMY40_RS16405"
                     /old_locus_tag="SAMN05216481_108135"
     CDS             complement(9438..10286)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907651.1"
                     /locus_tag="BMY40_RS16405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_108135"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_245770136.1"
                     /transl_table=11
                     /translation="MGRNNESARPAADGGGDRPGAWSAYGKPVRLFREREGLTQQSLAD
                     AIGYSLEQVASVEQGRRPAKAAFTGAAERVLKAGGALNTLQEDVDRAQSPAFFQDVAAL
                     ESEAISRFSYDQLVIPGLLQTEAYARALIQTHFPPLDDEIVEQRVAARLARQSILTRRT
                     PLTVFAFVIEEVALHRVVGGPQVMRAQLAHLLECSRMRHVGIQVMPNSRAAHSGLSGPM
                     VLLETHVRQRFVVIESQGVVSVRSGCREVGEFWLQYGMLRSQALNAEDSADLIKRAAGE
                     T"
     gene            10451..11041
                     /locus_tag="BMY40_RS27550"
                     /old_locus_tag="SAMN05216481_108136"
     CDS             10451..11041
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576585.1"
                     /locus_tag="BMY40_RS27550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_108136"
                     /product="ATP-binding protein"
                     /protein_id="WP_177214030.1"
                     /transl_table=11
                     /translation="MRHQSSAAGRPTTRPDDRPAEQAAGVPRFTARFSSTRRGARLARR
                     LAVQQFAEWTGLPHGCDAAHAVALVTAELAANAVTHGSLPGRDFRLTLLLSHEPAVHEP
                     VPHEPAAMRIEVTDTRPERLPRHPAADPGPPDEVSGRGLLLVAVYARRWGCEVRDAFTK
                     TVWAEVPLRQPSAFPTPHAGQASAQAVGRSDEV"
     misc_feature    11179..11195
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.63"
                     /tool="antismash"
ORIGIN
        1 gtcctcagac tgctgcgggg cagggacttc gagttcaccg tgaccgggac cgaacaggtc
       61 accgagcact atcggagggt ccggatgacg gacggcggga tgctcgcctc gaccggcgtc
      121 cacccgacga tgtgggtccg cctctggttc gagagcgggg gcaaaccgca ccagcgcgcc
      181 tacaccctgg tcgatcccga ccccgcggcc gggacgttcg ccctggagtt cgcactgcac
      241 gagggcatcg cctgcgactg ggcgcgcggc gcgcggccgg gcgacacgat cgaggccacc
      301 gtccagggca cggggttcac cgcgccgtcg cccgagccct cccggctcct ggccgtcggc
      361 gacccggcgt ccctgccggc ggtcaactcg ctgctggacg ccttccccgg cacgcccgcg
      421 accgtctggc tcgaaaccgc gcatccgcag gacgccgaac tgccgctgcg gctcgggccc
      481 ggcccgcacg aggtgcggtg gatcccgcgc cgggactccg gcgcccacct ggtgaccgag
      541 gtgaccacgg ccctgccgaa gctgctggag gacgagccgg acccctacgt ctggatcgcc
      601 tgcgacaccg ccacgacccg cgccctcgcc tcgtacgcgc gcaaggagct cggcgtgccg
      661 aaggacaggg tgcacgcgct gggctactgg cgcgcgacct gacgcgcggg ccggcgtacg
      721 ggccggtgcg cgggccgggg gcgaagccga cgcgccgcca cccggccggg cgtcacccgg
      781 gccgggggtg gcggtagtcc gacaagagca gcatcccgcg caatcgcccc ggaacgtcgt
      841 tacgttccga tcatgaccgt ctccgtgcgc cgtaccgccc gtcccctgac cgctctcgcc
      901 ctggtcgccg ccgcggcggc gggctgcgcg ggcccgggtg gcgataccac ccgggccggc
      961 gcctccccgg ccgcgccctc ctcaccggcc gctccggagg aagccggtga gggatcgggg
     1021 gaggagccgg aggagaagac ctccgggaag ggcgacttct ccgcctcccc ccgctcctcc
     1081 gtctcccgcc ccgagggcga cgaccgggac gcgtcgtcct ccggccggta cgccaccctg
     1141 cccgcctcca tcagcggccg tgtgcccgtc ttccggcacg gcccgcgcgc cgaggctccg
     1201 cgggacgccc gcggggagga gaaggccgag gaggacggcg agaaccgcga gaagcgggag
     1261 aagagcgaga acgccgagaa gacggagaaa accgagaagg cggagaaggc agagaaggcg
     1321 gagaagagcg gcgacaaggg ggacaaggtg gtggcgctga ccttcgacgc cgccctgtcc
     1381 gccgaggacc gcgagcgcgc cgaggaggac ggcaagcgcc acgacaaccc cgggctgatc
     1441 gccgcgctgc gccgcgagaa ggtccccgcg accgtcttca tgtcgggcct gtgggcgcag
     1501 acctacaagc accaggcgcg ctccatcggc cgtgacgacc tgttcgaggt cggcaacctc
     1561 tcctactccc accgcgcctt cacccgcgac tgccgcgacc tccccgcgct cgcgcccggc
     1621 aagcagctcg cggacgtgcg caaggggcag aaagccatcc gtgcggcggg ggtgaagaac
     1681 ctgacgccct acttccgctt ccccgggggc tgcttcgacg accgctcccg gcgggccgtc
     1741 gccccggcga gggtgacggt ggtggccggc gacgtcgtgg caccggacgc cgaggagaag
     1801 gatcccggga aggtggccga gcgggtgctg aaggacatca ggcccggttc ggtggtggtg
     1861 ctgcactgcg accgcgagaa ggcgccggcc acggagaagg ccgtccgccg gatcgtcccg
     1921 gagctgcggg agcgcggata ccgcatcgtg cacgtctcgg acatgatcgc ctcggccatg
     1981 ggccgggact gagtcccggc ccccgggcgg tcctgttcgg ctccgttcag caggagcagg
     2041 ccacgcgctc ggcctcttgc cactcgcaca ccgggcacag cgtcgcgccg cgcgtgctcg
     2101 ccgggtactc ggtcggctcg ccgcactgca cacacacggc ccgctcctcg cgcccggcgc
     2161 cgtcgagggt gccctcggcg cgctcctcgc ccccgtcgcg ggcggccgcc gtctcgggtg
     2221 gggtccagcc ggatacgaac tccatggggc cgagggtact cccggccccg cacgcccggg
     2281 acctcgtacg gaagcgggcg cggacgccgg tgcgccggct ggcacactgg ccccgtgagc
     2341 acctccgact cctcctccga cgccttctcc gagcccgccg ccacccgtgg ggcgaccgcc
     2401 cgggacgcgg ccccgcagta cgtcctgccg ctcgtcgtcc ggatcgagcg gtccgctccc
     2461 cccggcccgc ccgcccgcac cgacgccctc cggacggcgg cgcgcgcggt gctggtgatg
     2521 ctgtcggacg agcgcgcgac ggaccccggc ggcccctggc accaggcagt gcgcgactgg
     2581 caggacgggc gcatccgcaa ggtggtgcgc cgggcgcgcg gcgcggagtg gcggcgggcc
     2641 tgcgcgctgc cgggtgtcac ggtgaccggc gatggcgggc atccggccga ggtgcgggtg
     2701 ttcccgcccg taccgctgga cggctggccg aaggacctgg cccgcctcca ggtctccggc
     2761 accgaactgg aggaccccgt accgcccgta gcgccggaac ccggcacgcc cgtgctgtgg
     2821 ctcaatccgg aactggagat gtccgcaggc aaggcgatgg cccaggccgg tcacggcgcc
     2881 cagctcgcct ggtgggcgct ggacggcgcg gcgcgggccg cctggcgcgc ggccggcttc
     2941 ccgctcgcgg tccgtaccgc cccgcgcggg cggtgggagc ggctgacgga gtcgggcctg
     3001 ccggtggtgc gcgacgccgg cttcaccgag atcgccccgg gctcggccac ggtcgtggcc
     3061 gagcacccgg cgctcccggc gcgcccggtc cgtccggcgc cgcgcggctg agggactacc
     3121 gcccgccctc ggaggcccct cccccggcgc cctccgaggt ctctcccccg gcacgcccgg
     3181 cacgcccggc gcccccggtg ccttcgatgc cttccgcgtt ctcaccgtcc cgcttccccg
     3241 cgccccactc ccgcgtcctg cgctcgcgct ccatccgccg ccgcagcgcg gcgggcgggc
     3301 cggtgagctc ggggaagtcc gtccgcagcg cgcggaccag cgagtacatc atcaggaacg
     3361 ccaggacgaa gaacggcagg ccgagcaccg tgatcacctc ggcgagcgcg tccaggccgc
     3421 tcttgcccgt cgccgcgatg agcgtccccg cgaccacgcc ctcggtgatc gcccagaaca
     3481 cccgctgccg cgtcggcccg ccctcctccc gctgcccggc gctgagcatg tccaccacca
     3541 gggacgccga gtccgaggag gtggtgaaga agatgaccac gatcagcacc gagagggccg
     3601 aggcgagtgt cgtgagcggg tagttctcca ggaaggtgaa gagcgcgccg gggatgtcgc
     3661 cttcctccac cacctcccgc accagccccc ccgggccgtt cagctcgatg tcgatggcgg
     3721 acatcccgaa gacgctgaac cagacgatgg tgaaggccgt gggcagcgcg aggacgccca
     3781 ggacgaactc ccggatcgtg cggcccttgg agatacgggc gatgaagatg cccacgaacg
     3841 gcgaccaggt gatcgtccag gcccagtaga acaccgtcca ggtgccctgc cagccggtgt
     3901 cggcgaaggt gtcgttccag aaggccagcg acaccagcga ggacaggtag acgccgacgt
     3961 tctcgatgat ccccttggcc aggaacagcg tcgccccggt ggcgaagacg aagagcatca
     4021 gcccgatggc catgacgatg ttgatgttcg acagccgcct gatgcccttg tccaggccgt
     4081 agaccaccga ggtggtggcg atggcggtga tcacggtgat cagcagcacc tggaccagcg
     4141 agttctccgg caggccgaac agccgcgcca gtccgctgtt gatctgcatc gtccccaggc
     4201 cgatcgagac cgcgacgccg aagagcgttc ccatcaccgc cgcgatgtcc acgatccggc
     4261 ccgccgtgcc gtacacccgg tcccccagca gggggtacag gaccgagctg acccggaacg
     4321 gcatcccgcg cacgtagacg aagtaggcga acgtcagcga cggcaggcag aagatcgacc
     4381 aggtgtgcag cccgaagtgg tacagcgagt aggccatcgc catccgggcg gcctcggtgc
     4441 tctgcggctc gacgccctgc tggggcggct cggcgaagtg gctgatcggc tcggccacgc
     4501 cccagaacat caggatcgtg ccgatcccgg cggcgaacag catcgcgaac caggtccgcc
     4561 tgctgtactc cgggcggtcg tcctgggcgc ccaggcggat gtggccgtag cggctgacgg
     4621 cgatccagag cagatagacc aggaaggtcg tcacgcccag gatgtagaac cagcccagat
     4681 tggtgagcag ccagtccgag gccgtggcga agatccggtc cacctgctcc gtcagcgaga
     4741 tcgccgccac cacgaacagc acggtgagcc ctgcggaggt gaagaagatg accggatcgg
     4801 tacggagccc cagtaggcgc tgcagctttt ccagcatgta cctctccctg gaggactgat
     4861 ccgattttcg tcgttccccg gcccgatacg ggcgcgacac acgggcggcg gccgcgaacg
     4921 gcccggggcg gagccccggc gaaagctcag gtgaagctct gaactccacc gcgcgggatt
     4981 ggtgatccct ctgcccggag atgacaaccc cacacgggga ggagccgtcg aggggggcgg
     5041 gcgtggcacg actgggtacg gggatcggct ggcggccgaa gatcgcggac gcgatcgagt
     5101 cgctgccgga cgtcggctgg gtggaggtgg tcgcggagaa cgtctgcccc gaccacctgc
     5161 cgccggcgct ggtacggctg cgcgagcgcg gcacgaccgt ggtgccgcac ggggtggggc
     5221 tcgggctcgg gggcgcgcag cggcccgacc ccgcccggct ggcggcgctg gcgcgccggg
     5281 ccgaggcgct ggaggcaccg ctggtcaccg agcacatcgc gttcgtacgg gccggggggc
     5341 cgggagtcga ggggccggga gtcgaggggc cgggagtcga ggggccggga gtcgaggggc
     5401 cgggagtcga ggggccggtg atggaggccg ggcacctgct gccggtgccg cgcacccgcg
     5461 acgcgctgga cgtgctctgc gagaacgtga cgatcgccca ggacgcgctg cccgtgccgc
     5521 tggcgctgga gaacgtcgcg gccctgttca cctggcccgg cgaggagctg accgaggcgg
     5581 agttcctgac cgagctggtg gaccgcaccg gcgtcggcct gctcatcgac gtcgccaacc
     5641 tccacaccaa ccgcgtcaac cgcggcgagg acccgctggc cgtcctggac ggcctgccgc
     5701 tggacgcgct ggcctacgtg cacgtggcgg gcggcgtgga gcgcgacggg gtgtggcacg
     5761 acagccacgc ccacccggtg ccggagccgg tgctggagct gctggccgag ctgtgcgcgc
     5821 gcaccgatcc gccgggcgtg ctgctggaac gcgacgacga cttccccgcc gcggaggagc
     5881 tggccgccga actggccgcc gtacggggtg tgttcgaggg gacggcgcgg ggcgcgggag
     5941 gcacccgtgt ctgacaccgg tcctggctcc cgccctgtca ccgcccccgg cctcggcccc
     6001 ggccctgtca ccgaccccgg ctccggcacc gacgcggcac gccgccgcct cgccaccgtc
     6061 caggccgccc tgctgggcgc actggtcgcg gacggccccg tgcccgccgg cttcgacccc
     6121 gagcgactgg agatccagcg ccgggcgctg gtcgccaaac gggcctccgt catcgccggc
     6181 atcgcgcccg aactgccgga cgtcctgggc gatcaatacc gcccggcatt cgccgcctac
     6241 gcccgcggcc gccccatgac cggcgggtac cgcccggacg ccatgcgctt cgtggcccac
     6301 ctgctggact ccgaccccgc gctgtcccgg cgccggcggc gccgcctgcg ccgctggtac
     6361 cgcgaacgct ccggccaggc ccctcggggg tggggcctgg ccggtctcct cccccgccgc
     6421 ctgcgccgta cgcggccggg agcgtagcgg gcggcccgca ggcccgtacc gccctggccg
     6481 tcaccggcct cgccggccgg ggacaacggc tctgatcagc tctgctccgc cgcgagcacc
     6541 aggaacgcgg cccatccgcc ctggccaacc cgcactatgg gcccgtcgcc cgcagccttg
     6601 gagtcccgca cgtacacggc atccggccct gcggccacct ccacgcagtc cccaccgtcc
     6661 gatccgctgt ggctgctctt gaaccagtac agctcgcccg cgttgtcggt cacctgttcc
     6721 acgctcatgt ctctcccacc agccgctcga tgagtcgtgc agactcctcg acgttcaggg
     6781 cctgcgatcg cagcttgcca taacgcatgg agaaggctct gacctcggag acatccgata
     6841 cgacgcaccc gacaccttgg gactcgaagt agccgatgtc gcggtgctcc ttcgtctcca
     6901 ggatcacgaa cggaccgttc ttgcccgggt ggaatccgcg tccggcgggc atgatctgga
     6961 cctctacgtt ccgcagcgcg ttgacctcca gcagacgtcg ccactgctcg cgcatgacct
     7021 ccgggccgcc cacgggatcg cgcagcgcct cctcgctgat gatgaacgac agctccgcca
     7081 tcggtacgcg cgtcagcagc ttctggcggc tgagccgggc ctcggtgtgc tggtcgatgg
     7141 tctcctcgct cagcggcggg cagtgcccgc cgaagaccgc ccgtgcgtag ccctccgtct
     7201 gcaacagccc cggcaccagc agcggatcgt aggagaagcg gctcagcgcc tccgactcga
     7261 tgagcgcgaa gttgcggaag aagcgcggca gtttggcccg ttccacgtcg tcctggagga
     7321 cttccaggac gccccgcgct cccagcgcgc ggtccgccgc gatcgtgaag gcggccttcg
     7381 ccggccgccg tccctgctcg accgaggcga cctgctccag cgagtagccg atggcgtccc
     7441 cgagcttctg ctggctcaac ccggcctgct tgcggaggtg ttgaagcagt ctgccgtagg
     7501 ccgaccacac ccccggcggg tcgccgttct cctccttcac ccgtaccgcg cgaccgttcc
     7561 gcgtgtcccg caccgccctc acgccccttc gtccccgagt ccgtcccgct cgtcggctcc
     7621 cccggcggct tccgccgtcc gtcaccgccg gggccgtgtc caacgcggcc cgcgcaggcc
     7681 ggggcacggc ccgagacccg tacaacaccc gtacaagagg ccgttgtacg gctccccatc
     7741 gaccggcgga gacccggcca tacgtccgcc gcccgccgcc ttccacttcg cgggatcggt
     7801 aggaagggga ggggcacata cgtccaggcc atcccccctt cgggagcgct gggaggtaac
     7861 cgcccagcgt gaggccgtcc gttccgtgaa caccggcggc acggaacggt cttggccgga
     7921 aaacgcctgc gtcgcccgat cggcccaggg aaaggatgcg ctcgtcactc aaggcgacaa
     7981 gggggttgcg tgcggttcac gctgaggcgg aaacagccgc tcgaagaaga gtccggtgcc
     8041 tgcggcaagg ggctcggcga tctcggcgag gggatcggcc ggatcagccg cgagatcgcg
     8101 gagatccgca ccgcgctcgc cgaggcggcg gagaagccgc cgtcgcaggc gatgagcgga
     8161 gagctccaca gactccggag aaccgcctcc gacgggatca ggatcctcca ggaggagaac
     8221 cagacgcagc tacggcggca ggaggagatc ctcggccgcc tgggcgaact gcgcctggag
     8281 atcaccggcg gccgcaccgg ggcacacccg ccgcccgccc cgggctcccc cggcgccgac
     8341 gaacccggcg gggcggacgg caccggtgag gcgggagacg gaacggcggg gacggccggg
     8401 gcccagtcat tggcccactc ttcggccccg tcggacgaag cccccgagac acaggacagc
     8461 cgacggcagg aggagcgcat ggagcaggag caggacggcg taccggagcc cccggacggg
     8521 gcggctccct cccgggagac cgaggagacg ccggaagagc gcgtgaggaa ggtgaaggag
     8581 gccgctgagc aagccctccg gaaccgttcc gaggactccc gcggccgtac cgaccgcacc
     8641 gcaacgtccc cggtagcgga cgaccggcgg ctccacggcg tccacgcgga cggcacagac
     8701 ggccagcggc cgggacggca ggacggcggc cgggccgcgc cacgcgaccc gctggccgag
     8761 cacgcggaac tgctgctgaa cgccgccggg gtgtcgcgcg ccgaactggt ctgccaccgc
     8821 gacacctggg agttcctgct cgtacaggcc gtccggcatc ctcgcttccg cgttcccgga
     8881 gaggtcaaag acatcggtga cggaaaggtc gggacgcacc tgtccgggcc gtccctcatc
     8941 gccgccctga tcagcctctg gaagacccgc gaggagccga gcgaggaggt ggacgcggac
     9001 tgggcgatgg ccgccgtgct ctacgaccgc atccgcgacc ggctgaccga ggccgggccc
     9061 ggcgacggac gccgtaccgt ccgcatcgtg ctggacgacg gggacgggcc ggaggagacg
     9121 gcggtggccg ccgagacctc cgccgaggag gagccggagg ccgcgtaacc ccgcccgctc
     9181 acaccgctgc ggcccggacg gcgtccgggc cgcagcggtg cggaaccggg ttcagcgccc
     9241 ggccgcgagc gcgacgaaag cggcccaccc gtcgcggccg atccacagta cgggcccgcc
     9301 ggccgtcttg gagtcccgca cgtacacggc gtccgccccg gcggccacct ccacgcagtc
     9361 cccgccgttc gggccgctgt ggctgctctt ggaccaggac agctcaccgg gcgcgtcgcc
     9421 gccctgccgt tcgatgctca tgtctcccct gccgcccgct tgatgagatc ggccgaatcc
     9481 tcggcgttga gagcctgtga gcgcagcatc ccatactgga gccagaactc gccgacctcg
     9541 cggcaaccgg accggacgct caccacgccc tgcgactcga tcaccacgaa cctctgccgt
     9601 acgtgcgtct ccagcaggac catcgggccg ctgagcccgc tgtgggcggc cctgctgttc
     9661 ggcatcacct ggatgcccac gtgccgcatg cgggagcact ccagcaggtg ggcgagttgg
     9721 gcccgcatca cctgcgggcc gcccaccacg cggtgcagcg ccacctcctc gatgacgaac
     9781 gcgaagacgg tgaggggcgt cctgcgcgtc aggatggact gccgtgccag gcgtgccgcg
     9841 acgcgctgct cgacgatctc gtcgtcgagc ggcgggaagt gggtctggat cagggcgcgg
     9901 gcgtacgcct ccgtctggag gagtcccggg atcacgagct ggtcgtagga gaagcggctg
     9961 atcgcctccg actccagtgc cgccacgtcc tggaagaacg cgggcgactg ggcgcggtcc
    10021 acgtcctcct ggagggtgtt cagcgccccg ccggccttca gcacccgctc cgccgcgccg
    10081 gtgaacgcgg ccttcgccgg ccgccgtccc tgctcgaccg aggcgacctg ctccagcgag
    10141 tagccgatgg cgtccgccag ggactgctgg gtgaggccct cgcgttcgcg gaagaggcgg
    10201 acgggtttgc cgtaggcgct ccacgcgccg ggccggtccc caccgccgtc tgcggcgggc
    10261 ctggcactct cgttgttcct gcccatcgtt gtccccctgg cccgtcgcgg gtgcacctcc
    10321 atggcgaacc gcgccggtgc cggacggggc acgggcaccg gccgtacagc cgccgtacaa
    10381 ggggcttacg caggggcgtg tacggcggtc cgacctggcc gcgcgccccg cgtgacggga
    10441 cggttacggc atgcgacacc agagttccgc cgccgggcgg cccaccacac gccccgacga
    10501 ccgtcccgcc gaacaggccg ccggcgtacc gcggttcacc gcccgtttca gcagcacccg
    10561 gcggggcgcg cggctggcgc gcaggctcgc cgtgcagcag ttcgccgagt ggaccggcct
    10621 gccgcacggc tgcgacgccg cgcacgccgt cgccctggtg acggcggagc tggcggccaa
    10681 cgccgtcacc cacggcagcc ttccgggccg ggacttccgg ctgacgctgc tcctgtcaca
    10741 cgagccggcc gtgcatgagc ccgtcccgca cgagccggcc gcgatgcgga tcgaggtgac
    10801 cgacacccgg cccgagcggc tcccccgcca ccccgcggcc gacccggggc cgcccgacga
    10861 ggtgtcgggg cgggggctgc tcctggtcgc ggtgtacgcc cggcgctggg ggtgcgaggt
    10921 gcgggacgcg ttcaccaaga ccgtctgggc ggaggtgccg ctgcggcagc cgtccgcttt
    10981 cccgactccc cacgccgggc aggcgtcggc acaagccgtc ggccgctcgg acgaggtgtg
    11041 acttctccac cggccgtccg ccggccgaga aggctcggcg gcgcggccgg ggtgcggtgc
    11101 cgccatgtgc cggactcggt aggcagggcc tactgcgcag ccgtcctggt gagttccagg
    11161 gtcggcggcc cgtccggatc ggaattgaag tacaccttct ccgggccgct cgtgtagagc
    11221 tggacgccgc cgatcctgcc gagtccgccg tcctcgatcg acaggtagat gaagtcgctg
    11281 gacgcctggt cgtcgaggaa atcccactcg ccgctgaagt cgacgggatc ggagaccccg
    11341 tccatcgaca catcggtggc ggagaacgtc ccgtcggaac cgagcacgat ctgggcgccg
    11401 gtcgcctcgt tgcggtacac gccgggcagt tcgccggcat cgaccga
//
