LOCUS       NZ_FOET01000003        10375 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces radiopugnans strain CGMCC 4.3519, whole genome shotgun
            sequence.
ACCESSION   NZ_FOET01000003
VERSION     NZ_FOET01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces radiopugnans
  ORGANISM  Streptomyces radiopugnans
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900110735.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 01:56:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,515
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,315
            CDSs (with protein)               :: 5,315
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 41 of 125
            Pseudo Genes (incomplete)         :: 106 of 125
            Pseudo Genes (internal stop)      :: 6 of 125
            Pseudo Genes (multiple problems)  :: 26 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:58:30
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 103127
            Orig. end                         :: 113502
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOET01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10375
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10375
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10375
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5375](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5375)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(935..1735)
                     /locus_tag="BMY40_RS07925"
                     /old_locus_tag="SAMN05216481_10394"
     CDS             complement(935..1735)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /locus_tag="BMY40_RS07925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_10394"
                     /product="hypothetical protein"
                     /protein_id="WP_093657005.1"
                     /transl_table=11
                     /translation="MRRHSWVAQLGVLVIHNDPVVEAELWAMAPDGVTVHAARFESPTS
                     GGAEYTGTPWKEMVESPDVRRGLGQLGRMDLSAICLCFGSASFFGGPEFDEGFTAAARE
                     LAGGTEVYTAGQAIVGALAATGVERPLVVVPPWFTAPTFAAARGYLEAAGRGAADLLQY
                     QLDEEWREVRRHQLFDRGGRWEVRPDEVCRQVAGRFPKDADGVLIPGSGFRSFEAVEPL
                     EDELGVPVVTSNQACLWKLLDLTGLDAAVPGGGRLLGRRPAAAV"
     gene            complement(1732..3189)
                     /locus_tag="BMY40_RS07930"
                     /old_locus_tag="SAMN05216481_10395"
     CDS             complement(1732..3189)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060198.1"
                     /locus_tag="BMY40_RS07930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_10395"
                     /product="MFS transporter"
                     /protein_id="WP_093657007.1"
                     /transl_table=11
                     /translation="MTSPGTTGAAASRGATRAALAILAVAQFLLALDYSIVYIALPSVG
                     DEFGLTESSVQWVVSGYAVFFAGFLIVGGRASDRFGPRNLYVAAMLLFGVSSLAGGLAS
                     DTAVLLIARGAQGIAAAMLQPAVIALINTSFEAGPARNRALSVWGTVGASGLAAGVMAG
                     GLLTTVSWRWVFLINLPIAVLCVLGAPRLLPKSTAVPRRGTTLNVPNGVLCTGTVLTAA
                     LGLTQASGQGWSHWSTLAGLGTAAVLLAVFVVRERASRRPLVDPAIRRTRSLLVGCGST
                     ALYMASVGNMFFVVTLLLQELRGYGPLSAGLAFLPMAVAITVANSVAARLVDALGVRLT
                     LTLAFLADAAGLLLIAVQVGGDGYVTHLLPGLLVTGFAHGVTYVVMFIAGTADVEDRNQ
                     GVAGAMMTTAQYMSGALGIAVLVLVLGTDPGQDDFGRAFVATAVAALLGAALAWFGLAR
                     TSAARTGRDAAEEADAVAASPEGGRLS"
     gene            complement(3195..3899)
                     /locus_tag="BMY40_RS07935"
                     /old_locus_tag="SAMN05216481_10396"
     CDS             complement(3195..3899)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /locus_tag="BMY40_RS07935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_10396"
                     /product="HAD family hydrolase"
                     /protein_id="WP_093657009.1"
                     /sec_met_domain="HAD_2 (E-value: 3.8e-30, bitscore: 100.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGPYRAVLFDVDGTLVDTPGGMTKVLDAVVRETGREVDRERLRA
                     TVGRPLAASFATLLGLPEEHAEVAHAVERARRLFTETVIPAAADLVFPEVPPMLAELRE
                     RGLPLAVVTSKIRRSAVELLEAAGLLDSFDTLSCHGMAPRGKPAPDLALLACGELGVAA
                     GHCLVVGDAVDDMRMARGAGMAALGVGFGVATPRELLDAGALSVSGSVGELRAALSAAT
                     APAPLPLGSDQG"
     gene            complement(3896..4843)
                     /locus_tag="BMY40_RS07940"
                     /old_locus_tag="SAMN05216481_10397"
     CDS             complement(3896..4843)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269001.1"
                     /locus_tag="BMY40_RS07940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_10397"
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="WP_093657011.1"
                     /transl_table=11
                     /translation="MSAHDRTELFSISDEVADAVASGLPVVAMESSGVAHGLPYPMNLE
                     TALDMHKAIRAGGAVPARIGLFGGRFVIGMSDAMVERFATTPDLPKVSTRDIGPVLASK
                     GPGGTTVSAALVAAARAGIEVFAVAGIGGVHFGAQHSFDVSADLIEFTRHRVVTVCAGA
                     KTVLDPALTLEYLETQGVPVVGYRCDDFPAYYCVSSGRPNPRRTDDMDELAASVRLHWE
                     AGNRGGFLVTHPIAEQDALPSEHIYALIREKQAEAEREGVSGPAMTPRILSAVSAATQG
                     RTAEANRSVLLSTSALAAEVAVAMSRHGAPVGAA"
     gene            complement(5001..5375)
                     /locus_tag="BMY40_RS07945"
                     /old_locus_tag="SAMN05216481_10398"
     CDS             complement(5001..5375)
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958111.1"
                     /locus_tag="BMY40_RS07945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_10398"
                     /product="ectoine synthase"
                     /protein_id="WP_093657013.1"
                     /sec_met_domain="ectoine_synt (E-value: 5.2e-31, bitscore:
                     102.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRGKEEVEGVDWGNGLSYRFLVEKDRMGFTVAHTVVKAGSKSP
                     LHYRRHLEACYCIAGHGQVVTEDGTVHEIGPGTLYALDEHDKHYLIAAPDRDLELVSVF
                     NPPLRGDERHNFDAAEYSHY"
     misc_feature    complement(5367..5382)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.74"
                     /tool="antismash"
     gene            complement(5425..5673)
                     /locus_tag="BMY40_RS07950"
                     /old_locus_tag="SAMN05216481_10399"
     CDS             complement(5425..5673)
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PP-binding (17-76). E-value: 2.3e-08.
                     Score: 26.2. Matches aSDomain:
                     nrpspksdomains_BMY40_RS07950_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1147: putative acyl carrier protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018105939.1"
                     /locus_tag="BMY40_RS07950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_10399"
                     /product="acyl carrier protein"
                     /protein_id="WP_245769939.1"
                     /sec_met_domain="PP-binding (E-value: 1.4e-06, bitscore:
                     23.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTEGEIKRFVISNFLPDVPADQLSSDLDLLENGVVDSLGLLRLI
                     TWVGDEYGIPVADLDIAPHQFSSVEAIADFVRSARVG"
     aSDomain        complement(5446..5622)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BMY40_RS07950_PP-binding.1"
                     /evalue="2.30E-08"
                     /label="BMY40_RS07950_PP-binding.1"
                     /locus_tag="BMY40_RS07950"
                     /protein_end="76"
                     /protein_start="17"
                     /score="26.2"
                     /tool="antismash"
                     /translation="DVPADQLSSDLDLLENGVVDSLGLLRLITWVGDEYGIPVADLDIA
                     PHQFSSVEAIADFV"
     misc_feature    complement(5922..5937)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.18"
                     /tool="antismash"
     misc_feature    complement(5954..5970)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.76"
                     /tool="antismash"
     misc_feature    5966..5980
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.7"
                     /tool="antismash"
     misc_feature    complement(5985..5999)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.0"
                     /tool="antismash"
     misc_feature    complement(6046..6063)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 22.53"
                     /tool="antismash"
     gene            complement(6078..6230)
                     /locus_tag="BMY40_RS07955"
                     /old_locus_tag="SAMN05216481_103100"
     CDS             complement(6078..6230)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133205.1"
                     /locus_tag="BMY40_RS07955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_103100"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_245769940.1"
                     /transl_table=11
                     /translation="MPTELAALAAGVSRATVRKWASRGKLTRYGRPGRAEYDLEEIREI
                     LEGKR"
     misc_feature    6279..6294
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.49"
                     /tool="antismash"
     gene            complement(6290..6877)
                     /locus_tag="BMY40_RS07960"
                     /old_locus_tag="SAMN05216481_103101"
     CDS             complement(6290..6877)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BMY40_RS07960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05216481_103101"
                     /product="hypothetical protein"
                     /protein_id="WP_093657017.1"
                     /transl_table=11
                     /translation="MSNTPFRAPAGRDTVRGDLLRLRDLYEESIAAQASAARPPAERVA
                     GRRRTHRHRGIALDDRAVSVRSDVLAVLTSWTGMVADERAVPAPPDARVPSLVGFLEAN
                     LDWLLGHPAGADFAGETAELNAALCAVLDREPAPRDREVGTCAVPGCGRVVRATSAGRR
                     PEVRCEAGHMWQARHWLLLGRPPAAVSASSAP"
     gene            complement(6979..7887)
                     /locus_tag="BMY40_RS07965"
                     /old_locus_tag="SAMN05216481_103102"
     CDS             complement(6979..7887)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BMY40_RS07965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05216481_103102"
                     /product="hypothetical protein"
                     /protein_id="WP_093657019.1"
                     /transl_table=11
                     /translation="MMDSRFLRLDTEDLCALVSEFDSAQTVAEELLAHPDGGPGRHAGP
                     GGRLLELRGDTAVLRDPGGDLCALPASGLYACRVAALTALAANQLLDPGVVTASLIGSG
                     AAGRIQLLVIARSVPDVSHVAVHSPGGPHASPVDSGIAGVLEATGIGLTVAASPEEATF
                     GASLVVVTEPVVSEPCLGHLAPGSVLVNATGRSLREETTGCVSAVYVDDLGLLDAAGSG
                     PGRPGSAAGRVPVPAGGRLGVEADLRQVFTGEHPGRACADHVLLVELLSGAAPGTRLDV
                     ALAERIHRTALCHGLGALSPG"
     misc_feature    complement(7711..7713)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(7741..7743)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(7857..7871)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 15.93"
                     /tool="antismash"
     misc_feature    complement(8067..8082)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 16.93"
                     /tool="antismash"
     gene            8076..8279
                     /locus_tag="BMY40_RS07970"
                     /old_locus_tag="SAMN05216481_103103"
     CDS             8076..8279
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BMY40_RS07970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05216481_103103"
                     /product="hypothetical protein"
                     /protein_id="WP_093657021.1"
                     /transl_table=11
                     /translation="MSARRRFTATLLVAFAAVLGPTAAGMASQAGQQTGPQTAAAGGSA
                     YGCPSDALGNPIPGDCTEDTHW"
     misc_feature    8630..8645
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.27"
                     /tool="antismash"
ORIGIN
        1 gggaacgtcg cggcgatccg ctccagcagg ccgggcgggg cggcgtcccc ggtgagcagc
       61 acgtccctcg cctcggggaa gaccaggccg cgcacggccg ccacctcggt gacgagccgg
      121 aagaacagcg gcaccgcctg gagcacgtgg ggcgcgcggg agacgcacag gtcggccagg
      181 cgcaccccgt ccaccccgtg gtcggggtcg acgagttcgg cgcagccgcc cgccgcgagc
      241 gtggcccaca cgtccagcag gcagaggtcg aagttgagcg gggcgtagtt gagcacccgt
      301 gttccggggc cgatgccgaa gcggcccgcc gcccaggaca tgaagcggtc ggcgcccagg
      361 gagtcgagca ccaccgtctt gggcgtgccg gtcgacccgg aggtggtcaa cagcaggccc
      421 gcgccgggcg ggaggtcccc gccgccgtcc ggcgcggcgc cggacgggcg cgacacgacg
      481 gcgggaggac ctcccggacc ggcctcgccg gcgctcagga cgtgtgtgca gcccgcctgc
      541 gcgcacaggg aggcgagcgc ctgcgcgccc agaccggcgg acggcagcag gacccgccgt
      601 ccggcccgga agcaggcgat gaggagcgcg acggtctccg gcgacttccg cgcgggcacg
      661 cacaccggcg cgccgtcggc gatccgcagg gacaccagct ccgcgtgggc ggcgtcggcc
      721 caggcggaca gctccgcgta ccgcagtacg cggccgtgcc aggacagggc cggggcgtcc
      781 ggccgggtcc gcgcctgttc ggcgaagagt tccacgaacg atcgggacgg catggggtct
      841 tcccttcacg tcggacgggc gtggcggagc ggcccggacg gaccggcggg gcgggccgtc
      901 cgggcgggct cggccggagg tgcgggacgc cggctcacac cgcggcggcc ggccggcggc
      961 ccaggagccg gccgcccccg ggcacggcgg cgtccagccc ggtcaggtcg agcagcttcc
     1021 acaggcacgc ctggttggag gtcaccaccg gtacgcccag ctcgtcctcc agcggctcga
     1081 ccgcctcgaa ggagcggaac ccgctgcccg gtatcagcac gccgtcggcg tccttgggga
     1141 acctcccggc gacctggcgg cacacctcgt cggggcgcac ctcccagcgg cccccgcggt
     1201 cgaagagctg gtggcggcgg acctcccgcc actcctcgtc gagctggtac tggagcaggt
     1261 ccgcggcgcc cctgccggcc gcctccagat agccgcgcgc ggcggcgaag gtcggcgcgg
     1321 tgaaccacgg cggcaccacg accaggggcc gttccacgcc ggtggcggcg agggcgccca
     1381 cgatcgcctg gccggccgtg tacacctcgg tgccgcccgc cagctcgcgg gccgcggcgg
     1441 tgaagccctc gtcgaactcc gggccgccga agaagctggc cgagccgaag cacaggcaga
     1501 tcgcggacag gtccatccgg ccgagctggc ccagcccccg ccggacgtcc ggcgactcca
     1561 ccatctcctt ccagggggtg ccggtgtact cggcgccgcc gctggtcggg ctctcgaagc
     1621 gggccgcgtg gacggtgacg ccgtccggcg ccatggccca cagctcggcc tcgaccacgg
     1681 ggtcgttgtg gatcaccagg accccgagct gcgccaccca ggagtgccgc ctcacgacag
     1741 cctccctccc tcgggggagg cggcgaccgc gtccgcctcc tcggcggcgt cccgcccggt
     1801 ccgggccgcg gaggtgcgcg ccaggccgaa ccacgccagc gcggcgccga ggagggcggc
     1861 caccgccgtg gcgacgaacg cccggccgaa gtcgtcctgc ccgggatcgg tcccgagcac
     1921 caggaccagg acggcgatgc cgagcgcacc gctcatgtac tgggcggtcg tcatcatcgc
     1981 gcccgccacg ccctggttgc ggtcctccac gtcggccgtg cccgcgatga acatcaccac
     2041 gtacgtgacg ccgtgcgcga agccggtgac cagcagaccg ggcagcaggt gcgtcacata
     2101 gccgtccccg cccacctgga ccgcgatcag cagcaggccg gcggcgtcgg cgaggaaggc
     2161 cagggtgagg gtcagccgca cccccagcgc gtcgaccaga cgcgccgcca ccgagttcgc
     2221 cacggtgatg gccacggcca tcgggaggaa cgccagcccc gcggagagcg ggccgtagcc
     2281 gcgcaactcc tgcagcagca gcgtcaccac gaagaacatg ttcccgacgc tggccatgta
     2341 gagcgcggtc gagccgcagc ccaccagcag ggaccgggtg cgccggatcg ccgggtccac
     2401 cagcggccgc cggctggccc gctcccgcac cacgaacacg gccagcagga cggcggccgt
     2461 cccgagtccg gcgagcgtgc tccagtggga ccagccctgg ccggacgcct gggtgagccc
     2521 gagggccgcg gtgaggaccg tgccggtgca cagcaccccg ttgggcacgt tgagggtggt
     2581 gccgcggcgg ggcaccgccg tcgacttcgg cagcagccgg ggcgcgccca ggacgcacag
     2641 caccgcgatg ggcaggttga tcaggaagac ccagcgccag gagacggtgg tcagcagacc
     2701 gcccgccatc acaccggccg ccaggccgga ggcgccgacg gtcccccaga ccgacagcgc
     2761 ccggttgcgc gccggacccg cctcgaacga ggtgttgatc agggcgatga cggccggctg
     2821 gagcatggcc gcggcgatgc cctgggctcc gcgggcgatc agcaggacgg ccgtgtcgga
     2881 ggccagcccc ccggccagcg aggagacgcc gaacagcagc atcgcggcga cgtagaggtt
     2941 ccgggggccg aagcggtccg acgcccgtcc gccgacgatg aggaacccgg cgaagaagac
     3001 cgcgtagccg ctgacgaccc actggaccga gctctcggtc aggccgaact cgtcgccgac
     3061 gctgggcagc gcgatgtaga cgatggagta gtcgagggcg agcaggaact gcgccacggc
     3121 gaggatcgcc agtgcggcgc gggtggcgcc gcgggatgcc gcggcgccgg tggtacccgg
     3181 tgaagtcatg tggatcatcc ctggtcggag ccgagtggaa ggggggcggg cgcggtggcg
     3241 gccgagagcg ccgcgcgcag ctcgccgacc gagccggaca ccgacagcgc gccggcgtcc
     3301 agcagttcgc ggggggtggc gacgccgaac ccgacgccga gggccgccat gcccgcgccg
     3361 cgtgccatgc gcatgtcgtc caccgcgtcg ccgacgacga ggcagtgtcc ggcggccacg
     3421 cccagctcgc cgcaggcgag cagcgcgagg tccggggcgg gtttgcccct gggggccatg
     3481 ccgtggcacg agagggtgtc gaacgagtcc agcagcccgg cggcctccag cagttcgacc
     3541 gcgctgcgcc ggatcttgct ggtgacgacg gccagcggca ggccgcgctc gcgcagttcg
     3601 gcgagcatcg gcggcacctc cgggaagacc aggtcggccg cggccgggat gaccgtctcg
     3661 gtgaacagcc gccgggcgcg ctccacggcg tgcgccacct ccgcgtgctc ctcgggaagc
     3721 ccgaggaggg tggcgaacga ggccgccagg gggcggccga ccgtggcgcg cagccgctcc
     3781 cggtccacct cccggcccgt ctcccgcacc accgcgtcca gcaccttcgt catgccgccc
     3841 ggagtgtcca ccagggtgcc gtcgacgtcg aagagcaccg ctcggtacgg gccgctcacg
     3901 cggcacccac cggcgcgccg tgccggctca tggcgacggc cacctcggcc gcgagcgcgc
     3961 tggtggacag cagcacggag cggttcgcct cggcggtgcg gccctgggtc gcggccgata
     4021 ccgcgctcag gatgcgcggt gtcatcgccg ggccgctgac gccctcgcgc tcggcctcgg
     4081 cctgcttctc ccggatcagt gcgtagatgt gctccgaggg cagcgcgtcc tgctcggcga
     4141 tcgggtgggt gacgaggaac ccgccgcggt tcccggcctc ccagtgcagg cggacggagg
     4201 ccgcgagctc gtccatgtcg tccgtgcggc gcgggttggg ccgtccgctg gagacgcagt
     4261 agtaggcggg gaagtcgtcg cagcggtagc cgaccaccgg gacgccctgg gtctccaggt
     4321 actccagcgt cagcgccggg tccagcacgg tcttcgcccc cgcgcacacg gtgacgaccc
     4381 ggtgccgggt gaactcgatc aggtccgccg acacgtcgaa gctgtgctgg gcgccgaagt
     4441 gcaccccgcc gatgccggcg accgcgaaga cctcgatgcc cgcccgggcg gcggccacca
     4501 gtgcggcgga gacggtcgtg cctccgggtc ccttggaggc gaggacgggg ccgatgtccc
     4561 gggtgctgac cttgggcagg tccggcgtgg tggcgaaccg ctccaccatc gcgtcggaca
     4621 tgccgatcac gaaccgcccg ccgaagaggc cgatccgggc gggcaccgcg ccgcccgccc
     4681 ggatggcctt gtgcatgtcg agcgcggtct ccaggttcat cgggtacggc aggccgtggg
     4741 ccacgcccga ggactccatc gcgaccaccg gcagtcccga cgccaccgcg tcggccacct
     4801 cgtccgatat cgagaacagt tcggtccggt cgtgtgcgga catcggtcct tacctccttg
     4861 ctccggcccg ccgtcgggcc ctgggatggg aagcgctggt ggctgttgcg gtgggtggcc
     4921 ggtgcgcccg gggaggggtc gggcgcaccg gccggcctca cgacgccccc gtgcctgccg
     4981 ggggcgggtc cgtcgccgga tcagtagtgg gagtactcgg cggcgtcgaa gttgtgccgc
     5041 tcgtcgcccc gcagcggcgg gttgaagacg ctgaccagtt ccaggtcccg gtcgggcgcg
     5101 gcgatcaggt agtgcttgtc gtgctcgtcg agcgcgtaga gggttccggg cccgatctcg
     5161 tggaccgtgc cgtcctcggt gaccacctgg ccgtggccgg cgatgcagta gcaggcctcc
     5221 aggtgcctcc tgtagtgcag gggcgacttc gagccggcct tcacgacggt gtgggccacc
     5281 gtgaagccca tccggtcctt ctcgacgagg aaccggtagc tcagaccgtt gccccagtcg
     5341 acgccctcga cctcttcttt cccgcggaca atcatccttc ttctcctctt catggttccg
     5401 gacgcgacgg accgccgcgc cgagtcatcc gacgcgcgca ctccggacga aatccgcaat
     5461 ggcttcgacc gagctgaact gatgcggcgc gatatcgagg tcggcgaccg gaatcccgta
     5521 ctcgtcaccg acccaggtga tgaggcgcag cagaccgagg ctgtccacca ctccgttctc
     5581 cagcagatcg agatcggagg agagctggtc ggcggggaca tcggggagga aattgctgat
     5641 gacgaaccgc tttatctcac cttctgtgga cacggttctc ctctcgtggt tttcgcggag
     5701 ccgggcgggt gccgtggcca tggggggcgg ttcatggccg atgcgggaag gaaacccgcc
     5761 gttcagtgag gtgtggcgag cgccggcacc ggacggtgcg gacgcggggc cgggagcggg
     5821 aagaggcgtc gctccgggcg ggggaagccg gggaggaggg gatccgggaa gaagtgccgc
     5881 caaactcccg ggcgttcggg gccccggggc acaagtgtgc cgtgggaaaa gcgtgacacc
     5941 tcgcccgggc gtccgtcaac aacattccaa atcttttcca gttcaattgt ctggcgattg
     6001 tccgggcatg cgtgaggaat ccgcgccggg aaacaaccca tgccggatcc gctttacggg
     6061 gtttcatccg agccgcctca gcgttttccc tccagaatct cccggatttc ctccaggtcg
     6121 tactccgcgc gtcccgggcg gccgtaacgg gtcagttttc cgcggctggc ccacttgcgc
     6181 acggtggccc gggacacgcc ggccgcgagc gcggccagct ccgtgggcac cgtgcactgc
     6241 ggcctcccgg gccgcccgga tctcccgggc gtcacgggtg tcacgggcgt cacggcgcac
     6301 tgctcgcgga cacggcggca ggcggccgcc ccagcaggag ccagtgccgg gcctgccaca
     6361 tgtgcccggc ctcgcagcgc acctcgggcc ggcggccggc ggacgtcgcg cgcaccaccc
     6421 gcccgcagcc gggcaccgca cacgtcccca cctcgcggtc ccgcggggcc ggctcgcggt
     6481 ccaggaccgc gcacagcgcc gcgttcagct ccgccgtctc ccctgcgaag tcggctccgg
     6541 cggggtgccc gagcagccag tccaggttcg cctccaggaa ccccaccagc gacggcaccc
     6601 gcgcgtccgg gggcgccggc accgcccgtt cgtcggcgac catccccgtc caggaggtga
     6661 ggacggccag tacgtccgac cgcacggaca cggcccggtc gtccagggcg atgccgcggt
     6721 gccggtgggt acggcgccgg ccggccacgc gctccgccgg cgggcgggcg gcggacgcct
     6781 gcgcggcgat gctctcctcg tacaggtccc gcaggcgcag caggtcacct cggaccgtgt
     6841 cacggccggc cggtgcccgg aacggagtgt tcgacatacg cgtgtcctca cgacggaacg
     6901 gttgggggga tggggcgcgg ccgtgggacg cctccgcgcc cgggtggaac acacgggagg
     6961 agcggggtgc cgcggccgtc agccgggcga gagggcgccg aggccgtggc acagcgcggt
     7021 ccggtggatc cgttccgcga gcgccacgtc cagcctcgtc cccggggccg cgccgctcag
     7081 gagctccacc agcaggacgt ggtcggcgca ggcgcggccg gggtgttcgc ccgtgaagac
     7141 ctgccgcagg tcggcctcga cgccgaggcg gccccccgcc ggaacgggaa cgcgcccggc
     7201 cgcggagccg gggcggccgg gcccggaccc ggccgcgtcc agcaggccga gatcatcgac
     7261 gtagacggcg ctgacacagc ccgtcgtctc ctccctcaga ctgcggccgg tggcgttgac
     7321 cagcacggac cccggggcca gatgtcccag gcacggctcg ctcaccaccg gctcggtgac
     7381 gacgaccagg ctggcgccga aggtggcctc ctccgggctc gccgcgacgg tcagcccgat
     7441 gcccgtggcc tccaggacgc cggcgatccc gctgtcgaca ggcgaggcgt gcgggcctcc
     7501 cggcgagtgg acggcgacat ggctgacgtc cggcacgctg cgcgcgatca ccagcagctg
     7561 tatgcgcccg gcggcgcccg agccgatcag cgaggcggtg accaccccgg ggtccaggag
     7621 ctggttcgcc gccagcgcgg tcagggccgc gacccggcag gcgtacaggc ccgacgccgg
     7681 cagcgcgcac aggtcgccgc cggggtcccg taacaccgcc gtgtcaccgc gcagttccag
     7741 taatcgcccg ccgggtccgg cgtgccgccc ggggccgccg tccgggtgcg ccagcagttc
     7801 ctcggcgacc gtctgcgcgg agtcgaactc ggagacgagg gcgcacaggt cctccgtgtc
     7861 caacctgaga aagcggctgt ccatcatcgt ggcctcctcg catccggaga tcgtgcacac
     7921 gacaatgagg agcagggctc ccgatgctct gtcgatccgc tcccggtgcg ctgagtgccc
     7981 gccgggagcg aggagatggc gattcgatgg aatcctgcgc tccactcttc tcaccgaacc
     8041 gcaccaccac cgaccggcac catggaggga aaaacatgtc cgccagacgc cgtttcaccg
     8101 ccacgctcct cgtcgcgttc gcggccgtcc tcgggccgac cgcagcagga atggcgtccc
     8161 aggcaggtca gcagacgggc ccgcagacgg cggcggccgg gggatcggcg tacggctgcc
     8221 cctcggacgc cctggggaac ccgatcccgg gcgactgcac ggaggacacc cactggtgat
     8281 ccccgggccg gtgcgggcac gcgccgcgcg tgcccgcacc ggcccggctc acacggaggc
     8341 ggggccgccg acctccgcgc ccgagccgag cagctccccc gccggcagat cggggtgggc
     8401 ggcccgcagc tccgccagca gcttcgcgga ctcgcgcgag tcgaccgacg acagcagacg
     8461 tacggcgcgc tccaggtcgc cccgggcgga gtcctgtccg gcacggacct ccgacagcag
     8521 gacgagggcc ttcgcctgcc acagcaccga gcccagcttc ccgaagatct cgtgggccgc
     8581 ccgcagatgg ccggcggcgc tcgcctcgcc tccccgcgcc agcgcgatct cgccgagcgc
     8641 gtacagcgcc ttcgcctcga tgagctgctc ccccacccgg cgggccagcc ccagggcgtg
     8701 cgcgagggtg gtctcggcgg tgtccagccg gccctcccgg tgccgcagca gccccagccc
     8761 atagaccacg tggacctccc cgatgcggtc accggtgtcg cggaccatgc gcagcacccg
     8821 gtgcagcgcc tggcgggcga gatcgatccg gccggtggcc atgtacacct cggcgaagcg
     8881 gtgcacgacc tgcacctcca tcctcgggca cgacacctcc cggcagatgg ccagggcctc
     8941 gtccagcaac tcgcccgcgg tctgcgggtc cccctcgccc aggtggaact tggccaggct
     9001 gcgcaggatg tgcgcctccc ccacgcggtc gcccacggcg cgcatcgtcc gcagcgactc
     9061 gtcgtacctg tcgagcatcc tggggatgtc gccgtacagg ccgtcgacgt gcgcggcgtt
     9121 gcgcagcacc agggcgcagc cgtgcgcgtc gccctccgcc cggaaggcgg cgagcgcggc
     9181 ctcgaagcag taccgcgcct cggccatctg ggtctggaac atgtgcagcg tccccagcga
     9241 gtacagcatc gccgccgtgc ccgtgcggtt gccggcccgc tcggcggcct ccctggcgac
     9301 cgtggcgcac tcgcgccagt cgtcgaagta gcccttcgcc tcgaacaggg tgaccgcggt
     9361 cagcgccagg tcccagcaca gctcgtccag cccggcctgc gccgcctgcc gcacggcggc
     9421 cgtcagggcg cgccgctcgt cctcccacca gcacgccgga tcgcccatgc cgtccgggtc
     9481 ccagcccccc gccgggcgcc agcgcggagc gctgccgtgc aggatcgtgt agtccccgcc
     9541 gtactcgctg cggtgcgcct cctccgccag ggccagccag ccgcccagca ggcgggacag
     9601 ggccgccgcg cgctcgtgcc cgtcctcctc cgccgacagc cgctccccgg cgtagatccg
     9661 gatgaggtcg tggaagcggt agcggggcga acgggatccc gggtacacca cgacgtccag
     9721 gagctgcgcg tccaccagcg tgtccaggag ctccctgccc tcggccggcg agacgtccag
     9781 cagcgccgcc gcgttccagt ccgggaagtc gaggaccctg accatcgaca gacgccggaa
     9841 cagcctggcc gcggactcgc cgaggccgcg gtaggccagc tccatgttgg acctcaactc
     9901 cagcccatgg tgggcgagtt cgtccagcct gcgcgcctcg tcgcgcagcc gccgcaccag
     9961 gccctccatc tgccagtggg gccgcgaggc cagccgcgct ccggcgatcc gcagcgccag
    10021 cggcaggccg ccgcaggacg tcaccagctc cacggccgcc tcgcgttcgg cctccacacg
    10081 gtcctcaccg acgatcctgg tgagcagctc gatggacttg tcggtgtcga agacgtcgat
    10141 gtcgagccgg tgggcgccgg tcaggccgcc caggcgcttg cgactggtga cgatcaccgc
    10201 gcacgtggga ctgccgggca gcaggggccg tacctgctcc tccgaggtgg cgtcgtcgag
    10261 caccagcagc agccgcctgc ccgacagctt gctgcggaag agctcgacgc gctcgtccgt
    10321 gtcctccggc accgcggtgc ccggaacgcc cagcgcgcgc aggaagcggg ccagg
//
