LOCUS       NZ_FOET01000001        10405 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Streptomyces radiopugnans strain CGMCC 4.3519, whole genome shotgun
            sequence.
ACCESSION   NZ_FOET01000001
VERSION     NZ_FOET01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces radiopugnans
  ORGANISM  Streptomyces radiopugnans
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900110735.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 01:56:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,515
            CDSs (total)                      :: 5,440
            Genes (coding)                    :: 5,315
            CDSs (with protein)               :: 5,315
            Genes (RNA)                       :: 75
            rRNAs                             :: 4, 4, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 4, 5 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 41 of 125
            Pseudo Genes (incomplete)         :: 106 of 125
            Pseudo Genes (internal stop)      :: 6 of 125
            Pseudo Genes (multiple problems)  :: 26 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 05:58:30
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1025799
            Orig. end                         :: 1036204
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FOET01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    132..147
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    complement(375..393)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.24"
                     /tool="antismash"
     misc_feature    473..488
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.95"
                     /tool="antismash"
     gene            542..2548
                     /locus_tag="BMY40_RS04455"
                     /old_locus_tag="SAMN05216481_101901"
     CDS             542..2548
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174265.1"
                     /locus_tag="BMY40_RS04455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_101901"
                     /product="beta-galactosidase"
                     /protein_id="WP_093655454.1"
                     /transl_table=11
                     /translation="MVRLAYGGDYNPEQWPEEVWHEDVALMREAGVTTVSVGIFSWAMI
                     EPAEGVFDFGRLDRIIELLHGAGIGVDLATPTVVPPAWFYRAHPEALPVSREGVRWEFG
                     SRGAICHNSPAYQQASARIARALGERYGRHPAVVMWHVHNEYGVPVLECYCETSAAAFR
                     TWLRERYTTLERLNAAWGTAFWGQTYGDWEEIAPPRATPTVCNPAQRLDYARFASDSAL
                     ANFRRERDLLHELSPGVPVTTNFMVSPTQCDTIDYWAWGREVDIVTNDHYLVAEDERNH
                     VNLSMAADLTRSVAGGEPWLLLEHSTSGVNWQPRGIAKRPGEMARNSLAHIARGSEGAM
                     FFQWRASRSGAEKFHSAMVPHAGTDSRIWREVVSLGADVAALEEIRGTRTVADVAVVWD
                     WQSWWAQKLEWRPSQDLDARERAEAWYAALFDRHLTADFVPPRTDPAKLAAYPLVVVPA
                     LYLASAEAGEDLRAYVAGGGTLVVSYFSGIVDEHDAVHPGPHPGALREVLGLTVEEFQP
                     LRAGRTVTVLGSGVPSLEADVWAETVVARGAETVWSYGGGPAAGGPAVTRNRLGEGTAW
                     YVSTRLGPGGLDAVLAAACADAGIAPREEIPRDVEVVRRSGQSGTYLFVLNHTGADAKV
                     PLEASGTELLGGARVAGRLAVPAGGVRVVRLDG"
     gene            2684..3805
                     /locus_tag="BMY40_RS04460"
                     /old_locus_tag="SAMN05216481_101902"
     CDS             2684..3805
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135913.1"
                     /locus_tag="BMY40_RS04460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_101902"
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="WP_093655456.1"
                     /transl_table=11
                     /translation="MRRRTVLRLGAVALAAPALATAAPGTAAAAPRLRVRGADVSSLAK
                     GEDLGGVYHHADGRRDDAVRILAGAGVNWGRLKVWVDPADGYHTRPHILRTARRLHAAG
                     MKLLVDFHYSDTWADPGKQFKPAAWEDLHGAALERAVRDHTADILGALRAQGTPAAMVQ
                     IGNEINGGMLWPDGSTDDWPRLAGLLTAGAEAARAASPGIRVALHLAEGGRGADTRWWF
                     DNAVAHGVPFDAIGLSFYGYWHGTLADLQANLNDVSARYRRDVFVAETAYPFRLDSEDF
                     LENIIDRPSELVPGYPATPEGQAAWLRDITAVVAAVPGGRGLGLFWWEPTWTAVPGNGW
                     DPADPASGNGWENQALFDYDSRALPALEVLGRG"
     gene            complement(3964..4863)
                     /gene="thpD"
                     /locus_tag="BMY40_RS04465"
                     /old_locus_tag="SAMN05216481_101903"
     CDS             complement(3964..4863)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671935.1"
                     /locus_tag="BMY40_RS04465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_101903"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_093655457.1"
                     /sec_met_domain="PhyH (E-value: 4.8e-39, bitscore: 130.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGASEVITPRQDPTVWSAPGTPGPIEAADLA
                     AYDRDGFLAIDQLITPDEVEVYNRELNRLVSDPLIRADERSIVEPKSQEIRSVFEVHKI
                     SEVFANLVRDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYETNGALMIMPGSHRTFLGCAGATPKDNYKKSLQMQDAGTPSDEA
                     LTRLAGEHGIRLFTGKPGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENTAVEPFA
                     APIPRPEFLGARDFTPVR"
     gene            complement(5001..5405)
                     /locus_tag="BMY40_RS04470"
                     /old_locus_tag="SAMN05216481_101904"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141824.1"
                     /locus_tag="BMY40_RS04470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_101904"
                     /product="ectoine synthase"
                     /protein_id="WP_093655460.1"
                     /sec_met_domain="ectoine_synt (E-value: 2e-54, bitscore:
                     178.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLQEIEDTDRHVRSASGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHVEAVLCVEGEAELTNDETGEKHWITPGTMYLLDGHEKHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEPDES"
     gene            complement(5537..6805)
                     /gene="ectB"
                     /locus_tag="BMY40_RS04475"
                     /old_locus_tag="SAMN05216481_101905"
     CDS             complement(5537..6805)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 2.1e-72.
                     Score: 235.9. Matches aSDomain:
                     nrpspksdomains_BMY40_RS04475_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835756.1"
                     /locus_tag="BMY40_RS04475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_101905"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_093655462.1"
                     /sec_met_domain="Aminotran_3 (E-value: 8.4e-76, bitscore:
                     251.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSIFQTLESEVRSYCRGWPTVFDRARGSYMYDEDGHAF
                     LDFFAGAGSLNYGHNNPVLKRALLDYLERDGVTHGLDMSTTAKRAFLETFQEVVLRPRG
                     LDHKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDNYLDGKVEDFLWFERLLQDSGSGLNRPAAVIVETVQGEGGVNVARA
                     EWLRALADLCKRQDMLLIVDDIQMGCGRTGGFFSFEEAGITPDIITVSKSISGYGLPMA
                     LTLFRPELDVWEPGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTLARGEQVEAALC
                     AIAGEHRGGVAGFRGRGLVWGLAFPDAGRAGWVCSRAFELGLLVETSGPESEVVKLLPA
                     LTISPEELDEGLRTLARAVRETA"
     aSDomain        complement(5711..6721)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BMY40_RS04475_Aminotran_3.1"
                     /evalue="2.10E-72"
                     /label="BMY40_RS04475_Aminotran_3.1"
                     /locus_tag="BMY40_RS04475"
                     /protein_end="365"
                     /protein_start="28"
                     /score="235.9"
                     /tool="antismash"
                     /translation="FDRARGSYMYDEDGHAFLDFFAGAGSLNYGHNNPVLKRALLDYLE
                     RDGVTHGLDMSTTAKRAFLETFQEVVLRPRGLDHKVMFPGPTGTNAVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYLDGKVEDFLWFER
                     LLQDSGSGLNRPAAVIVETVQGEGGVNVARAEWLRALADLCKRQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGITPDIITVSKSISGYGLPMALTLFRPELDVWEPGEHNGTFRGNNPAFV
                     TAAAALDTYWADGQMEKQTLARGEQVEAALCAIAGEHRGGVAGFRGRGLVWGLAFP"
     gene            complement(6886..7422)
                     /gene="ectA"
                     /locus_tag="BMY40_RS04480"
                     /old_locus_tag="SAMN05216481_101906"
     CDS             complement(6886..7422)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545554.1"
                     /locus_tag="BMY40_RS04480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_101906"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_093655464.1"
                     /transl_table=11
                     /translation="MPEGLKLDAPRVEDGAAIWRIARDSRVLDLNSSYSYLLWCRDFAA
                     TSAVARDGDGEPVGFITGYVRPRHPRTLVVWQVAVDHGQRGRGLAAAMLDGLTTRAAEE
                     LGIDGIETTITPDNTASNRLFTSYAERHGAPVRREVLFEGALFPEEGHEPEVLYRIGPV
                     PALASTATAPAAAGG"
     misc_feature    7494..7510
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.6"
                     /tool="antismash"
     misc_feature    complement(7639..7656)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.31"
                     /tool="antismash"
     gene            complement(7820..8548)
                     /locus_tag="BMY40_RS04485"
                     /old_locus_tag="SAMN05216481_101907"
     CDS             complement(7820..8548)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976943.1"
                     /locus_tag="BMY40_RS04485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_101907"
                     /product="hypothetical protein"
                     /protein_id="WP_093655466.1"
                     /transl_table=11
                     /translation="MTDRPDVVARLRVAADGHRPDRARMWARVSARLPAGSREEAGRVR
                     PRPRRAWPRMAGAAGGLVAVLAAGGLAVDRVAGGGEPWGERAPAGAGVRDGGPPSAAPS
                     AGGGEPGPREDRLRSRGVLGPGNNAHWAQSGVVLHCGERLASLTVELRVAPAGGVRHTG
                     FWSTLPADDFEVTVREEGGALVYRWTLRKGRAVPPGEHRFVGQYGHAEGLRDAGRDSYT
                     VHATGPGGPVSAEGDFRRVP"
     gene            complement(8545..9105)
                     /locus_tag="BMY40_RS04490"
                     /old_locus_tag="SAMN05216481_101908"
     CDS             complement(8545..9105)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433895.1"
                     /locus_tag="BMY40_RS04490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_101908"
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="WP_093655468.1"
                     /transl_table=11
                     /translation="MDAPTEEFHAFFDRYYADLSRFAYLLLGDSEGADDLAADALVAVW
                     RRWDSVRADGHPLAYARSVVAGMARTRQRSLVRERRRIALFWSHRPDRIDDPDVPAVLD
                     VRDALARLPLRKRACVVLRHAFDLSEKETARTLGISVGTVKSQTSRGIAELERMLAPAR
                     TGPERAYETVRALRAGPAGRSGW"
     gene            9201..10298
                     /locus_tag="BMY40_RS04495"
                     /old_locus_tag="SAMN05216481_101909"
     CDS             9201..10298
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017238577.1"
                     /locus_tag="BMY40_RS04495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216481_101909"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_093655470.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.3e-19, bitscore:
                     66.5, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSERGNFHIRGRVLTGPGPDDVRDEMWVVDGRITYERPTGPLETA
                     TVEGWVLPGLVDAHCHVGLDAHGAVDDATSEKQALTDRDAGTLLIRDAGSAADTRWIDD
                     REDLPRIIRAGRHIARTRRYIRNYAHEIEPGDLAAYVRGEARRGDGWVKLVGDWIDREQ
                     GDLAPCWPRGAVEAAIAAAHEEGARVTAHCFAEDSLRDLVEAGIDCVEHATGLTEETIP
                     LFAERGVAIVPTLVNIATFPRLAEGGEKKFPRWADHMRRLHERRYDTVRAAYDAGIPVF
                     VGTDAGGSLPHGLVADEVAELVAAGIPPVDALSATTWAARAWLGRPGLEEGAEADLVVY
                     EADPRADVRVLAAPRAVLLRGRAVR"
ORIGIN
        1 ccttctccgc gccggccacc tcgcccgata tgtcggccag cacgtcgttg ccgaccaggg
       61 tgggcagcgc ctggtactcg gcctgcacca ggtcgggggc ctcaccagcc ttggccgcgg
      121 tgatggtctt ggtcaccagg tcgtccccgc tggcctgctt cttgaccacc accttgacgt
      181 ccgggttctc ctcgttccag agggcgacga ccttgtccat gccgggcgcc caggcccagt
      241 aggtcagttc cgccgggccg gaggcggcgg agtcgtcgcc cgaggagccg cagccggtga
      301 ggacggctgc cgccagagcg gccgcggtgc acgccgcgac ggtgcggtgg ttcatgtttc
      361 ctccctgaca aggcgctgtg aacgtgcaca gtagagaaac ccgactgact cttgtcaatg
      421 gttcggggct tgatgttttc taggtgatgc agtcgacgtg cggctgtgaa cgttcccagt
      481 tctttcccag ttccttcccg gtccggctca ctcgggccgg gacttcgacc caggaggtcg
      541 tatggtgcgc ctcgcctacg gcggcgacta caaccccgag cagtggcccg aggaggtctg
      601 gcacgaggac gtcgcgctga tgcgtgaggc cggtgtgacc acggtcagcg tcggtatctt
      661 ctcctgggcg atgatcgagc ccgccgaggg cgtcttcgac ttcggacggc tcgaccggat
      721 catcgagctg ctgcacgggg ccggcatcgg cgtcgacctg gccaccccca ccgtggtgcc
      781 gcccgcctgg ttctaccgcg cccaccccga ggcgctgccc gtcagccggg agggcgtgcg
      841 ctgggagttc ggctcgcgcg gcgcgatctg ccacaactcg cccgcctacc agcaggcctc
      901 cgcccggatc gcccgcgcgc tgggcgagcg ctacggaagg catcccgcgg tcgtgatgtg
      961 gcacgtccac aacgagtacg gcgtacccgt cctggagtgc tactgcgaga cctccgccgc
     1021 cgccttccgc acctggctgc gggagcgcta caccaccctg gagaggctca acgccgcctg
     1081 gggcaccgcc ttctggggcc agacctacgg cgactgggag gagatcgccc cgccccgggc
     1141 gacccccacc gtctgcaacc ccgcccagcg cctggactac gcccggttcg cgagcgacag
     1201 cgccctggcg aacttccgcc gcgagcgcga cctgctgcac gagctgtccc cgggcgtccc
     1261 ggtcaccacc aacttcatgg tctcgcccac ccagtgcgac accatcgact actgggcctg
     1321 gggccgcgag gtcgacatcg tcaccaacga ccactacctg gtggccgagg acgagcgcaa
     1381 ccacgtcaat ctgtcgatgg ccgccgacct gacccgctcg gtcgccggcg gcgagccgtg
     1441 gctgctgctg gagcactcca ccagcggcgt caactggcag ccgcgcggca tcgccaagcg
     1501 ccccggcgag atggcccgca actcactggc ccacatcgca cgcggctcgg agggggcgat
     1561 gttcttccag tggcgcgcct cccgctccgg cgcggagaag ttccactcgg cgatggtgcc
     1621 gcacgccggc accgacagcc ggatctggcg cgaggtggtc tcgctcggcg cggacgtcgc
     1681 cgcgctggag gagatccgcg gcacccgcac ggtcgcggac gtggcggtcg tgtgggactg
     1741 gcagtcgtgg tgggcgcaga agcttgagtg gcgccccagc caggacctcg acgcccgcga
     1801 gcgcgccgag gcctggtacg ccgcgctctt cgaccgccat ctgaccgccg acttcgtccc
     1861 ccctcggacc gatccggcga agctggccgc gtacccgctg gtggtggtcc ccgccctcta
     1921 cctggcctcg gcggaggccg gtgaggacct gcgcgcctac gtcgccggcg gcggcaccct
     1981 ggtggtctcc tacttctccg gcatcgtcga cgaacacgac gccgtccacc ccggccccca
     2041 cccgggcgcg ctgcgggagg tgctcgggct gacggtcgag gagttccagc cgctgcgggc
     2101 gggccggacg gtgaccgtcc tcgggtccgg ggtcccctcg ctggaggccg acgtgtgggc
     2161 cgagaccgtc gtggcgcggg gcgccgagac ggtgtggtcc tacggcggcg gcccggccgc
     2221 gggcggtccc gccgtcaccc ggaaccggct gggcgagggc accgcgtggt acgtctcgac
     2281 gcgcctgggc ccgggtgggc tcgacgccgt gctggccgcc gcctgcgcgg acgcgggcat
     2341 cgccccgcgc gaggagatcc cccgcgacgt ggaggtggtg cgccgctccg gacagtccgg
     2401 cacctacctc ttcgtcctca accacaccgg cgccgacgcc aaggtgccgc tggaggcgtc
     2461 cggcaccgaa ctgctcggcg gcgcccgcgt ggccggccgg ctcgcggtgc cagccggtgg
     2521 ggtccgcgtg gtccgcctcg acggctgagc cccggcgctc cggtacggcc gggccggtcg
     2581 ccgagggccg gcggagggtc ggtgggtgcc ggcggaggac accgcaagag cagtgagaac
     2641 accacgagaa caccacgaga gaaccggaag gaagggcaca gccatgcgca gacgcaccgt
     2701 cctgcggctg ggagcagtcg ctctcgccgc ccccgccctg gccaccgccg cccccggcac
     2761 ggccgcagcc gctccgcgcc tgcgcgtccg cggcgccgac gtctcctccc tcgccaaggg
     2821 cgaggacctc ggcggcgtct accaccacgc cgacggccgc agggacgacg cggtacggat
     2881 cctggcggga gccggggtca actggggccg tctgaaggtc tgggtggatc cggccgacgg
     2941 ctaccacacc aggccccaca tcctgcggac ggcccggcgg ctgcacgcgg cgggcatgaa
     3001 gctcctggtc gacttccact actccgacac ctgggccgac cccggcaagc agttcaagcc
     3061 cgcggcctgg gaggacctcc acggagccgc gctggagcgg gcggtgcgcg accacaccgc
     3121 cgacatcctg ggcgcgctgc gggcgcaggg cacaccggcg gccatggtcc agatcggcaa
     3181 cgagatcaac ggcggcatgc tgtggcccga cggctcgacc gacgactggc cccgcctggc
     3241 cggcctgctc acagccgggg ccgaggccgc ccgcgccgcc tcgcccggca tccgggtcgc
     3301 cctccacctg gccgagggcg ggcggggcgc cgacacccgc tggtggttcg acaacgcggt
     3361 ggcgcacggc gtgcccttcg acgccatcgg cctgtccttc tacggttact ggcacggcac
     3421 gctggccgat ctccaggcca acctgaacga cgtctcggcc cgctaccgca gggacgtctt
     3481 cgtcgcggag accgcctatc cgttccggct ggacagcgag gacttcctgg agaacatcat
     3541 cgaccggccc tccgagctgg ttcccggcta ccccgcgacc cccgaggggc aggccgcctg
     3601 gctgcgcgac atcacggcgg tcgtggccgc cgtaccgggc gggcggggac tggggctgtt
     3661 ctggtgggag ccgacctgga ccgccgtgcc cggcaacggc tgggacccgg ccgacccggc
     3721 ctccggcaac ggctgggaga accaggcgct cttcgactac gacagccgcg ccctcccggc
     3781 gctggaggtc ctgggccgcg gctgacccgt acccgccgcc cgagggatcc gcgggcgggt
     3841 acggcgaagg ggcggggccg ccgtggcggc cccgcccctt gctccgcgtt cctcgcgtcc
     3901 cgccgaccgc gcaccgtgcg gtccggcggt cggcggcacg ctccccaccc cgcggggcgg
     3961 tggtcacctc acgggggtga agtcccgtgc gccgaggaac tccgggcgcg ggatgggcgc
     4021 cgcgaacggc tccaccgcgg tgttctccac gctgttgaag acgatgaaga cgttgctgcg
     4081 cgggtagggg gtgatgttgt ccccggagcc gtgcatgcag ttgcagtcga accaggtcgc
     4141 cgaaccgggc ttgcccgtga acagccggat gccgtgctcg ccggcgagcc gggtcagcgc
     4201 ctcgtcggac ggcgtgcccg cgtcctgcat ctgcagggac ttcttgtagt tgtccttcgg
     4261 ggtggccccc gcgcagccga ggaacgtcct gtgcgaacca ggcatgatca tcagggcgcc
     4321 gttggtctcg tagttctcgg tcagggcgat cgagacggag accgtccgca tgttcggcag
     4381 accgtcctcg gcgtgccagg tctcgaagtc ggagtgccag tagaaccccg atgccccgaa
     4441 gcccggcttg acgttgatcc ggctctggtg gacgtacacg tccgagccca ggatctgacg
     4501 ggcgcgtccc accacccgcg ggtccctcac caggttggcg aagacctcgc tgatcttgtg
     4561 gacctcgaag acggagcgga tctcctggga cttcggctcc acgatcgaac gctcgtccgc
     4621 gcggatcagc gggtccgaga ccagccggtt cagctcccgg ttgtagacct ccacctcatc
     4681 gggggtgatg agctggtcga tcgccaggaa gccgtcccgg tcgtaggccg cgaggtccgc
     4741 ggcctcgatc ggtcccggcg tgccgggggc ggaccacacc gtggggtcct gccgcggggt
     4801 gatcacctcg gaggctcccc gggtcgggta gagatccgcg gtgcgttcgg gtgcggtggt
     4861 catggttctg ccttcctctc ctcatcgtgc ggccctgttc ccgcgtgtgg ccgcttcgtc
     4921 gcgggttctc tcgccagggc ctcccgtgtg gtctcggctg ccgctcgcac cggtccgtcc
     4981 ggatcggccg gtgcggacgg tcagctctcg tccggttcgg tgagcagggg gtagacgccg
     5041 ttctcgtcgt ggtcctcgcg gccggtgacc ggcgggttga agacgcagac gcaacggaag
     5101 tccgtcttgg gacgaagtgt gtgcttctcg tgtccgtcca gcaagtacat ggtgccgggg
     5161 gtgatccagt gcttctcgcc ggtctcgtcg ttggtgagct cggcctcgcc ctccacgcag
     5221 aggacggcct ccacatggtt ggcgtaccac atcgaggtct ccgtgccggc gtagagcacg
     5281 gtctcgtgca gcgagaagcc cacccgctcc ttcgcgagca cgatccgctt gctctcccag
     5341 gtgccggagg cggacctgac gtggcggtcg gtgtcctcga tttcctgcag cgatcggacg
     5401 atcacagtgc tgaggtgcct ttcctgtcag aggcccatcc gcggaaaaac tcgcgggatg
     5461 ggccattcgg gtggtgcgtg cggcgaccgg acgcccccgg cgccggtcgg gcgccgggga
     5521 cgggcggtgc cgcgtgtcag gcggtctcgc ggaccgcgcg ggccagggtg cgcaggccct
     5581 cgtccagctc ctcgggggag atcgtcagcg ccgggagcag cttgacgacc tcgctctcgg
     5641 ggccggaggt ctcgaccagc agccccagct cgaaggcgcg ggagcacacc cagccggcac
     5701 gccccgcgtc ggggaacgcc agcccccaca ccagaccgcg gccccggaag ccggccaccc
     5761 ccccgcggtg ctcgccggcg atcgcacaca gggcggcctc gacctgctcg ccgcgggcca
     5821 gcgtctgctt ctccatctgc ccgtcggccc agtaggtatc cagggccgcc gccgcggtga
     5881 cgaaggccgg gttgttgccg cggaaggtgc cgttgtgctc gccgggctcc cagacgtcca
     5941 gctcgggccg gaacagggtc agcgccatcg gcagcccgta gccgctgatg gacttggaga
     6001 ccgtgatgat gtcgggggtg atcccggcct cctcgaagga gaagaagccg ccggtgcggc
     6061 cgcagcccat ctggatgtcg tcgacgatca gcagcatgtc ctggcgcttg cacaggtcgg
     6121 ccagggcgcg cagccactcg gcccgggcga cgttgacgcc accctcgccc tgcacggtct
     6181 ccacgatcac ggccgccggc cggttcaggc cggagccgga gtcctgcagc agccgctcga
     6241 accacaggaa gtcctcgacc ttgccgtcga ggtagttgtc gaacggcatc ggggtgccgt
     6301 gcaccagggg gacgccggcg ccggcgcgct tgaaggcgtt gccggtcacc gccagggagc
     6361 ccagcgacat gccgtggaag gcgttggtga aggagacgat cgactcccgg cccttgacct
     6421 tgcgggccag cttcagggcc gcctcgacgg cgttggtgcc ggtcgggccg gggaacatca
     6481 ccttgtggtc caggccgcgc ggacgcagga ccacctcctg gaaggtctcc aggaaggccc
     6541 gcttggcggt ggtggacatg tccaggccgt gggtgacgcc gtcgcgctcc aggtagtcca
     6601 gcagggcgcg tttgagcacc gggttgttgt gcccgtagtt cagtgacccg gcgccggcga
     6661 agaagtcgag gaacgcgtgg ccgtcctcgt cgtacatgta gctgccgcgc gccctgtcga
     6721 agacggtggg ccagccgcgg cagtagctgc gcacctccga ttcgagggtc tggaagatgc
     6781 tcaggtcggg ctgggtgatg gtcacagcga tctcctggga gatggattgc gagaggagtg
     6841 agaggtctgg ggtgccgggg cgcggtgcgg cgcgccgcgc ggcggtcagc cgcctgcggc
     6901 ggccggtgcg gtcgcggtgg aggccagggc tgggacgggc ccgatccggt acagcacctc
     6961 gggctcgtgt ccctcctccg ggaacagcgc gccctcgaag aggacctcgc gccgcaccgg
     7021 tgcgccgtgg cgctccgcgt aggaggtgaa cagtcggttg gaggcggtgt tgtcaggggt
     7081 gatcgtcgtc tctatcccgt cgatgccaag ctcctcggcg gcacgggtgg tcagcccgtc
     7141 gagcatcgcc gcggccaggc cgcgtccgcg ctgcccgtgg tcgacggcga cctgccagac
     7201 gaccagggtc cgcgggtgcc gcggccggac gtagccggtt atgaaaccga ccggctcacc
     7261 gtcgccgtcg cgcgccaccg ccgaggtggc ggcgaagtcg cggcaccaca ggaggtagct
     7321 gtaggaggag ttgaggtcga gtacccggga gtcgcgcgcg atccgccaga tcgctgcgcc
     7381 gtcctccacg cggggcgcgt cgagcttcag tccctccggc atttcgacgg tctttctgtg
     7441 ggcacctgca tggtctgctt gtgcggcggt catgcaaatc caatttaccc agagcgaact
     7501 caaatcacat cgaggacagg ggttacgcgg gcgcgctttc tgtgttatcg cgcgtgggtg
     7561 cgcacgcgca cgaaagccgg tgcgttatgc ctggtatgcc ccctattttc cgggcaaaga
     7621 ggaatcgatg ttgagtccgt cacagatcca taacgagcgc ggaacgtgtc cgaaatacgc
     7681 ggttcgccga tatcgaatct tagtgtttaa ggaccgtctg agcgggcatg aggatatggg
     7741 attctattca gatcaggtgc ccgtaaattc atcttcgatt catggaagat atcccagcga
     7801 aaccggatcg tgaggttctt cagggaactc ggcggaagtc cccctccgcg gacaccggcc
     7861 cgccggggcc cgtggcgtgg acggtgtagg agtcccgccc cgcgtcccgc agcccctccg
     7921 catggccgta ctggcccacg aacctgtgct cgcccggcgg caccgcgcgc cccttccgca
     7981 gcgtccagcg gtacaccagc gcgccgccct cctcgcggac ggtcacctcg aagtcgtccg
     8041 ccggcagggt gctccagaag ccggtgtgcc gtacgccccc ggccggcgcg acgcgcagct
     8101 ccacggtgag ggaggccagc cgctcgccgc agtgcaggac gacaccgctc tgcgcccagt
     8161 gcgcgttgtt gccggggccc agcacccccc gggagcgcag ccggtcctcc cgcgggcccg
     8221 gctcgccccc gccggcggac ggagcggcgg acggcggccc tccgtcccgg actccggctc
     8281 ccgccggtgc ccgctccccc cacggctccc cgccgccggc cacccggtcg acggccagcc
     8341 cgcccgcggc gagcacggcc accagcccgc cggcggcccc ggccatccgc ggccaggccc
     8401 ggcggggacg cgggcggaca cggccggcct cctcccggga cccggccgga aggcgcgcgg
     8461 acacccgggc ccacatgcgg gcccggtccg gacggtgccc gtcggcggcc accctgagcc
     8521 gtgcgaccac atcggggcga tcggtcacca accgctccgt cccgcgggcc cggcgcgcag
     8581 cgcccgtacg gtctcgtacg cgcgctccgg ccccgtccgg gccggcgcca gcatccgctc
     8641 cagttcggcg atgccgcgcg aggtctggct cttgacggtg ccgaccgaga tgcccagggt
     8701 gcgggcggtc tccttctccg acaggtcgaa ggcgtgccgc agcaccacac acgcccgctt
     8761 gcgcagcggc agccgggcga gcgcgtcgcg cacgtccagc accgcgggca cgtcagggtc
     8821 gtcgatgcgg tcgggccggt gcgaccagaa cagggcgatc cggcggcgct cgcgcaccag
     8881 gctgcgctgc cgggtgcggg ccatgccggc caccacgctg cgcgcgtacg ccagcgggtg
     8941 cccgtcggcg cgcacgctgt cccaccggcg ccacaccgcg acgagcgcgt cggcggcgag
     9001 gtcgtcggct ccctcggagt ctcccagcag caggtaggcg aagcgggaca ggtcggcgta
     9061 gtaccggtcg aagaaggcgt ggaactcttc ggtgggggcg tccacgcggc ctcctcaggg
     9121 ctcgatggat cctgcgaagt gatgtgccac gaccccctgc ggctgcgtga gcgtagcagg
     9181 ggactcgtac agtgcggcat atgagcgagc gcgggaattt ccacatccgg ggacgggtcc
     9241 tgaccgggcc cggccccgac gacgtccggg acgagatgtg ggtcgtggac gggcggatca
     9301 cctacgagcg gcccaccggg ccgctggaga cggccacggt cgagggctgg gtgctgcccg
     9361 ggctggtgga cgcgcactgc cacgtcgggc tcgatgccca cggcgccgtc gacgacgcca
     9421 ccagcgagaa gcaggcgctc accgaccgcg acgccgggac gctgctgatc cgggacgcgg
     9481 gatcggccgc cgacacccgg tggatcgacg accgcgagga cctgccgcgc atcatccggg
     9541 ccggccgcca catcgcacgc accaggcgct acatccgcaa ctacgcccac gagatcgagc
     9601 ccggggacct ggcggcctac gtgcggggcg aggcgcggcg cggcgacggc tgggtcaagc
     9661 tcgtcggcga ctggatagac cgcgagcagg gcgatctggc cccctgctgg ccgcgcggcg
     9721 cggtggaggc cgccatcgcg gccgcccacg aggagggcgc gcgggtcacc gcccactgct
     9781 tcgccgagga ctcgctgcgc gatctggtgg aggccgggat cgactgcgtc gagcacgcca
     9841 ccggtctgac ggaggagacc atcccgctct tcgccgagcg gggcgtcgcc atcgtcccca
     9901 ccctggtcaa catcgccacc ttcccccggc tcgccgaggg cggcgagaag aagttcccgc
     9961 gctgggccga ccacatgcgg cggctccacg agcgccgcta cgacaccgtg cgcgcggcgt
    10021 acgacgccgg catcccggtc ttcgtcggca cggacgccgg cggctcgctg ccgcacgggc
    10081 tggtcgccga cgaggtcgcg gagctggtgg cggcgggcat cccgccggtg gacgccctgt
    10141 cggcgaccac ctgggcggcc cgcgcctggc tggggcgccc ggggctggag gagggcgccg
    10201 aggcggatct ggtggtgtac gaggcggatc cgcgggccga cgtacgggtg ctggccgcgc
    10261 cgcgggcggt actgctcagg gggcgcgccg tgcggtgagg cgcgcggtga ggcgcgcggt
    10321 gaggcatgcg gtgaggcgcg cggggtgtcc gcggtggagg agggcgtatg cccgccctgt
    10381 ggagacgcgg tgcgataccc atgat
//
