GCF_900107375.1 - 25 region(s)

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Location: 1 - 22,495 nt. (total: 22,495 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

BLV01_RS24820: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Ligases: ligase

BLV01_RS24815: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.77	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.75	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002071.2	0.73	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001133.4	0.70	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.77	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.75	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002071.2	0.73	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001133.4	0.70	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 22495:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys) + (Ile - Thr - Gly - Ser - Orn)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS24815: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (762..1177): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: LWVSFDLSVYDGDSFFSGDIGLGGATEATVWSNF
Nearest Stachelhaus code(s):: Cys DLFDFSLVWK (82% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLV01_RS24820: Ile - Thr - Gly - Ser - Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (568..961): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: LNNSFDASTMEGFVMCGGEIHCYGPTETTTFALT
Nearest Stachelhaus code(s):: Ile DAMFMGCTFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1648..2061): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGNQIFGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2750..3155): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDIAALELQALCGGEWNLYGPTETTIWSSI
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3815..4219): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWITFDVSVWEWHFFCSGEYNLYGPTETAIEASA
Nearest Stachelhaus code(s):: Ser DVWHFSLIEK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4884..5291): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QVLAFDMSVEEGNLAIGGEWNAYGPTEATVNACL
Nearest Stachelhaus code(s):: Orn DMENMGAVNK (85% 8Å match)
Stachelhaus code match:: 90% (moderate)

NZ_FNPT01000028.1 - Region 1 - redox-cofactor

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Location: 25,552 - 47,874 nt. (total: 22,323 nt) Show pHMM detection rules used

redox-cofactor: (cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

BLV01_RS23230: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: amidase back translocase

BLV01_RS23250: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

BLV01_RS23290: amidase

smcogs: SMCOG1105: amidase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000936.5	0.41	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0002561.3	0.39	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001444.5	0.37	other (shikimate-derived)	caboxamycin	Streptomyces sp. NTK 937
BGC0000313.4	0.36	NRPS	beauvericin	Beauveria bassiana
BGC0001080.5	0.36	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0002492.3	0.34	NRPS	myxochelin B, myxochelin A	Myxococcus xanthus DK 1622
BGC0001221.5	0.34	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0002324.3	0.33	NRPS, PKS	myxochelin B, myxochelin N, myxochelin O, myxochelin P, myxochelin Q, myxochelin A	Archangium violaceum
BGC0001100.5	0.33	NRPS, PKS	lankacidin C	Streptomyces rochei
BGC0000934.4	0.32	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000936.5	0.41	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0002561.3	0.39	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001444.5	0.37	other (shikimate-derived)	caboxamycin	Streptomyces sp. NTK 937
BGC0000313.4	0.36	NRPS	beauvericin	Beauveria bassiana
BGC0001080.5	0.36	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0002492.3	0.34	NRPS	myxochelin B, myxochelin A	Myxococcus xanthus DK 1622
BGC0001221.5	0.34	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0002324.3	0.33	NRPS, PKS	myxochelin B, myxochelin N, myxochelin O, myxochelin P, myxochelin Q, myxochelin A	Archangium violaceum
BGC0001100.5	0.33	NRPS, PKS	lankacidin C	Streptomyces rochei
BGC0000934.4	0.32	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

Additional weak hits (2):

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.56 out of 29.65

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.47 out of 33.14

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: PqqD_RRE; Location: 3 - 92; Score: 106.5; RRE type: Stand_Alone_Lasso_RRE; Location: 37 - 91; Score: 26.6

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FNPT01000008.1 - Region 1 - NRPS,transAT-PKS

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Location: 146,814 - 198,155 nt. (total: 51,342 nt) Show pHMM detection rules used

Region on contig edge.

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: PPTase acylase acyltransferase

BLV01_RS14605: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

BLV01_RS14630: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLV01_RS14635: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Hydrolases: hydrolase

BLV01_RS14580: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: dehydratase

BLV01_RS14615: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

BLV01_RS14620: 8-amino-7-oxononanoate synthase

smcogs: SMCOG1109: 8-amino-7-oxononanoate synthase

BLV01_RS27125: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000357.5	0.82	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001825.4	0.61	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.59	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.57	NRPS	AbT1	Aureobasidium pullulans
BGC0002286.3	0.57	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.57	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.56	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.56	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.56	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000357.5	0.73	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001273.3	0.62	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.62	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0000307.4	0.62	NRPS	AbT1	Aureobasidium pullulans
BGC0002286.3	0.62	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (11):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.62 out of 26.12

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.72 out of 26.12

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.55 out of 18.57

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.63 out of 18.57

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.42 out of 21.55

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.74 out of 20.01

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.79 out of 25.88

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.51 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.85 out of 29.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.18 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 146813 - 198155:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - mal - ccmal) + (D-X - D-Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 167345 - 198155:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - mal - ccmal) + (D-X - D-Ser)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS14630: mal - mal

PKS_AT (5..279): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 128.7
Methoxymalonyl-CoA: 49.0
inactive: 41.8
Methylmalonyl-CoA: 36.6
Propionyl-CoA: 33.0
2-Methylbutyryl-CoA: 14.7
trans-1,2-CPDA: 10.8
fatty_acid: 7.4
Ethylmalonyl-CoA: 4.0
Isobutyryl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

PKS_AT (314..596): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 37.8
inactive: 32.6
Methoxymalonyl-CoA: 24.2
Methylmalonyl-CoA: 15.7
Isobutyryl-CoA: 10.1
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

BLV01_RS14635: X - Ser - X

Search NORINE for peptide: strict or relaxed

AMP-binding (218..607): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FHIAFDASVAESAFLTAGSVNAYGPTETTILSTA
Nearest Stachelhaus code(s):: Tyr DAAAIAAICK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1723..2123): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWLTFDVSVMEWNFFASGEYNLFGPTETAIEASG
Nearest Stachelhaus code(s):: Ser DVWHFSLIEK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (3264..3603): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FWLAFDASIGEGAFLTAGSVNAYGPTETTVLSSA
Nearest Stachelhaus code(s):: R-bmeTrp DAGALGVVLK (47% 8Å match)
Stachelhaus code match:: 80% (moderate)

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator

NZ_FNPT01000009.1 - Region 1 - NRPS

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Location: 1 - 31,626 nt. (total: 31,626 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase aminotransferase

BLV01_RS14640: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

BLV01_RS14655: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: esterase

BLV01_RS14675: thioesterase

smcogs: SMCOG1004: thioesterase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.71	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.71	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (17):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.83 out of 26.85

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.39 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.67 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.36 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 21.28 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.73 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 18.52 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.27 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.42 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.99 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.32 out of 26.06

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.36 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.59 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.85 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.54 out of 30.25

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.90 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.45 out of 26.06

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 31626:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Dab - Ser - Dab)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS14640: Dab - Ser - Dab

Search NORINE for peptide: strict or relaxed

AMP-binding (690..1091): Dab

nrpys: Dab

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NSKAFDISVWQTEPLPTGEMNAYGPAECSDDVAF
Nearest Stachelhaus code(s):: Dab DIWELTADDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1728..2134): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFICSGEHNLYGPTEAAVDVTA
Nearest Stachelhaus code(s):: Ser DVWHISLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2789..3189): Dab

nrpys: Dab

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NSKAFDISVWQTEPLPTGEMNAYGPAECSDDVSF
Nearest Stachelhaus code(s):: Dab DIWELTADDK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description

NZ_FNPT01000035.1 - Region 1 - NRPS

Download region SVG

Location: 1 - 32,654 nt. (total: 32,654 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

BLV01_RS24595: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

Hydrolases: esterase

BLV01_RS24590: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase

BLV01_RS24600: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS24605: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001825.4	0.71	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001133.4	0.68	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.68	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001825.4	0.71	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001133.4	0.68	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.68	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 23.18 out of 28.19

Confidence: medium

Additional weak hits (17):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.46 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.02 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.53 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.10 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.86 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.90 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.57 out of 26.06

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.85 out of 25.88

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.73 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.38 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.44 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.51 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.56 out of 28.14

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.04 out of 21.84

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.55 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.00 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.22 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 32654:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asp - X) + (D-X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS24600: Asp - X

Search NORINE for peptide: strict or relaxed

AMP-binding (513..900): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YGVAADLGHTAGKIMFGGEFNHYGPTETTVGATC
Nearest Stachelhaus code(s):: Asp DLTKMGHVGK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1641..2033): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAVTFDMSVLECVALLGGDLNGYGPTEASVIVTA
Nearest Stachelhaus code(s):: Ile or Val DFLCLGVVIK (59% 8Å match); Val DALWLGGVFK (41% 8Å match)
Stachelhaus code match:: 70% (weak)

BLV01_RS24605: X

AMP-binding (546..945): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HGAAVDTTLEQADLVVGGDINAYGPTEATISSHY
Nearest Stachelhaus code(s):: Arg DTEDIGAITK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

TFBS definitions

Regulator	Description
ZuR variant 1	Zinc-responsive repressor

NZ_FNPT01000006.1 - Region 1 - NRPS-like,RiPP-like

Download region SVG

Location: 1 - 61,675 nt. (total: 61,675 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

BLV01_RS11565: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

Transferases: sulfotransferase

BLV01_RS11780: glutathione S-transferase

smcogs: SMCOG1193: glutathione S-transferase

Hydrolases: esterase protease

BLV01_RS11600: thioesterase

smcogs: SMCOG1004: thioesterase

BLV01_RS11790: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: dehydratase

BLV01_RS11740: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Isomerases: isomerase

BLV01_RS11745: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

BLV01_RS11570: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS11735: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS26260: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: transport

BLV01_RS11720: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.32	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.31	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.31	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.31	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.30	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.30	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.30	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.29	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.29	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001168.5	0.27	NRPS	Livipeptin	Streptomyces lividans 1326

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.66	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.66	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.65	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.40 out of 19.26

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.53 out of 20.97

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.75 out of 23.05

Confidence: medium

Additional weak hits (12):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.50 out of 26.33

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.91 out of 19.26

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.62 out of 32.93

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.72 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.85 out of 32.74

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.86 out of 27.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.50 out of 24.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.26 out of 29.65

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.27 out of 23.43

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.19 out of 18.57

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.39 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

BLV01_RS11570: X

AMP-binding (1..382): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LDFSQRLSVY-GVAITGMALEAYGVTETGMIAWN
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

BLV01_RS11735: X

AMP-binding (44..441): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AGQSHHITQASSLSMAGGD------------AMA
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

BLV01_RS11740: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (409..798): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: ARYSFAFGLQNIFAVAAGELDSLGSTEA--FSTY
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A

NZ_FNPT01000006.1 - Region 2 - lanthipeptide-class-ii

Download region SVG

Location: 105,981 - 128,857 nt. (total: 22,877 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

BLV01_RS12030: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000528.5	0.45	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002698.2	0.43	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000503.5	0.42	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001210.5	0.39	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002346.3	0.38	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000517.4	0.38	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000516.4	0.37	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0000527.5	0.36	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0001229.4	0.36	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000528.5	0.45	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002698.2	0.43	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000503.5	0.42	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001210.5	0.39	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002346.3	0.38	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000517.4	0.38	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000516.4	0.37	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0000527.5	0.36	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0001229.4	0.36	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BLV01_RS12030

allorf_106121_106327

Leader:

MPNSGEPKPSTIGLRDAWAVATHPGRSERVFDQSDAMSMRRAFRLLAFMGM

Core:

KPGNFITPRGHCSRPGR

Core with dehydrations:

KPGNFIDhbPRGHCysDhaRPGR

antiSMASH-DB 4.0 matches for allorf_106121_106327

100.0%

Lanthipeptides: allorf_106121_106327_lanthipeptide

1

KPGNFITPRGHCSRPGR

17

KPGNFITPRGHCSRPGR

1

KPGNFITPRGHCSRPGR

17

22.9%

NZ_CP113114.1and 2 others:

NZ_FZDV01000015.1 Lanthipeptides: CFW84_RS08635_lanthipeptideNZ_JALDTZ010000003.1 Lanthipeptides: M1F37_RS03135_lanthipeptide

Lanthipeptides: OTU45_RS07045_lanthipeptide

2

PGNFITPRGHCS

13

PGN I+ GHCS

19

PGNTISCNGHCS

30

19.4%

NC_018750.1and 1 other:

NZ_CP029195.1 Lanthipeptides: allorf_7265782_7265925_lanthipeptide

Lanthipeptides: allorf_0633709_0633852_lanthipeptide

2

PGNFITPRGHCSRPG

16

P F++ HC RPG

4

PRRFLSRPLHCQRPG

18

with 1 more sequence matches

19.4%

NZ_CP095046.1:

Lanthipeptides: MUN79_RS04140_lanthipeptide

2

PGNFITPRGHCS

13

PG F+T G C+

8

PGMFLTISGECN

19

allorf_106167_106274

Leader:

MLGPLRPIRGA

Core:

RSASSINPMRCRCGAHFVCLHSWA

Core with dehydrations:

RDhaADhaDhaINPMRCysRCysGAHFVCysLHDhaWA

antiSMASH-DB 4.0 matches for allorf_106167_106274

100.0%

Lanthipeptides: allorf_106167_106274_lanthipeptide

1

RSASSINPMRCRCGAHFVCLHSWA

24

RSASSINPMRCRCGAHFVCLHSWA

1

RSASSINPMRCRCGAHFVCLHSWA

24

18.5%

NZ_MOWR01000001.1:

Lanthipeptides: allorf_337897_338127_lanthipeptide

6

INPMRCRCGAHFVCL

20

+N + R +H++CL

3

LNILESRTDSHYICL

17

18.2%

BGC0001155.5 (AGY49587.1):

Lanthipeptides: allorf_3534805_3534990_lanthipeptide

9

MRCRCGAHFV------CLHSW

23

+RCRCG CL+ W

3

LRCRCGRAVARTRACGCLNGW

23

with 1 more sequence matches

15.9%

NZ_CP017171.1:

Lanthipeptides: allorf_7652578_7652769_lanthipeptide

12

RCGAHFVCLHSWA

24

RCGA C+H A

25

RCGAMSPCVHIEA

37

MIBiG 4.0 matches for allorf_106167_106274

16.7%

Thiopeptide: GE2270

11

CRCGAHFVC

19

C C F C

2

CNCVCGFCC

10

allorf_107263_107406

Leader:

MGSPGIRASAARGG

Core:

VRGCRMRWRRAARTGYRARRAGCVESDASDRIC

Core with dehydrations:

VRGCysRMRWRRAARDhbGYRARRAGCysVEDhaDADhaDRICys

antiSMASH-DB 4.0 matches for allorf_107263_107406

100.0%

Lanthipeptides: allorf_107263_107406_lanthipeptide

1

VRGCRMRWRRAARTGYRARRAGCVESDASDRIC

33

VRGCRMRWRRAARTGYRARRAGCVESDASDRIC

1

VRGCRMRWRRAARTGYRARRAGCVESDASDRIC

33

84.8%

NZ_CP067396.1:

Lanthipeptides: allorf_5380741_5380884_lanthipeptide

1

VRGCRMRWRRAARTGYRARRAGCVESDASDRI

32

VRGCRMRWRRAARTG RARRAGCVESD +RI

1

VRGCRMRWRRAARTGCRARRAGCVESDRLNRI

32

84.8%

BGC0002144.3 (RCCGEPOP_RS37510):

Lanthipeptides: allorf_5693634_5693777_lanthipeptide

1

VRGCRMRWRRAARTGYRARRAGCVESDASDRI

32

VRGCRMRWRRAARTG RARRAGCVESD +RI

1

VRGCRMRWRRAARTGCRARRAGCVESDRWNRI

32

MIBiG 4.0 matches for allorf_107263_107406

15.2%

LAP: phazolicin

21

AGCVESDASDR

31

A C D+S R

1

ATCARCDSSSR

11

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.04 out of 24.78

Confidence: medium

Additional weak hits (16):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.78 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.18 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.62 out of 20.01

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.30 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.97 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.18 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.29 out of 29.38

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.51 out of 20.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.54 out of 27.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.60 out of 35.63

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.52 out of 29.25

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.81 out of 18.57

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.26 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.33 out of 33.14

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

allorf_106121_106327 - Class II

Cleavage pHMM score: -5.00

RODEO score: 0

Monoisotopic mass: 1843.0 Da

Molecular weight: 1844.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1862.1 Da N = 1

allorf_106167_106274 - Class II

Cleavage pHMM score: -6.10

RODEO score: 0

Monoisotopic mass: 2616.2 Da

Molecular weight: 2618.0 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2636.1 Da N = 1

allorf_107263_107406 - Class II

Cleavage pHMM score: -5.80

RODEO score: 0

Monoisotopic mass: 3785.9 Da

Molecular weight: 3788.3 Da

Number of bridges: 3

TFBS definitions

Regulator	Description
LexA variant 2	Repressor of DNA damage response

NZ_FNPT01000006.1 - Region 3 - NRPS,lanthipeptide-class-ii

Download region SVG

Location: 169,628 - 224,436 nt. (total: 54,809 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase aminotransferase methyltransferase transferase

BLV01_RS12275: methyltransferase

smcogs: SMCOG1089: methyltransferase

BLV01_RS12295: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

BLV01_RS12335: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

BLV01_RS12430: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: esterase hydrolase

BLV01_RS12315: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

BLV01_RS12380: enterobactin/ferric enterobactin esterase

smcogs: SMCOG1098: enterobactin/ferric enterobactin esterase

BLV01_RS12440: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: cyclase

BLV01_RS12405: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: ligase synthase

BLV01_RS12330: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

BLV01_RS12345: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS12350: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS12355: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLV01_RS12420: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.41	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.37	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.37	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.37	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.37	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000342.4	0.36	NRPS	enniatin	Fusarium equiseti
BGC0001825.4	0.36	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.35	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000528.5	0.35	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001166.5	0.35	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.70	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.68	NRPS	HC-toxin	Alternaria jesenskae
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BLV01_RS12405

BLV01_RS12410

Leader:

MEDSTMQQNDDVVSGWLNGSDSVDGQDNPAGSLFIEGQAAAEAAMTGTGMRATLGA

Core:

CGTLGGTSCSYAGGCLCC

Core with dehydrations:

CysGDhbLGGDhbDhaCysDhaYAGGCysLCysCys

antiSMASH-DB 4.0 matches for BLV01_RS12410

100.0%

Lanthipeptides: BLV01_RS12410_lanthipeptide

1

CGTLGGTSCSYAGGCLCC

18

CGTLGGTSCSYAGGCLCC

1

CGTLGGTSCSYAGGCLCC

18

94.4%

Lanthipeptides: BV903_RS23035_lanthipeptide

1

CGTLGGTSCSYAGGCLCC

18

CGTLGGTSCSY GGCLCC

1

CGTLGGTSCSYNGGCLCC

18

77.8%

NZ_LMHM01000017.1:

Lanthipeptides: ASD86_RS21615_lanthipeptide

1

CGTLGGTSCSYAGGCLCC

18

CGTLG T+CSY GGC CC

1

CGTLGKTTCSYGGGCACC

18

with 9 more sequence matches

72.2%

NZ_CP076103.1:

Lanthipeptides: KME82_RS02935_lanthipeptide

1

CGTLGGTSCSYAGGCLCC

18

CGTLG T+CS+ GGC CC

1

CGTLGKTTCSFGGGCACC

18

44.4%

NZ_JALBFU010000002.1:

Lanthipeptides: MD221_RS08055_lanthipeptide

7

TSCSYAGGCLCC

18

T+CS+ GGC CC

1

TTCSFGGGCACC

12

38.1%

NZ_CP011130.1and 1 other:

NZ_KQ061218.1 Lanthipeptides: WN54_RS23350_lanthipeptide

Lanthipeptides: LC55x_RS02895_lanthipeptide

1

CGTLGGTSCSYAGGCLCC

18

CGTLGGT+CSY GGCLCC

25

CGTLGGTTCSYGGGCLCC

42

35.7%

NZ_CP023465.1and 1 other:

NZ_CP094357.1 Lanthipeptides: IEQ11_RS02850_lanthipeptide

Lanthipeptides: CNO08_RS02770_lanthipeptide

1

CGTLGGTSCSYAGGCLCC

18

CGT GGT+CSY GGCLCC

25

CGTFGGTTCSYGGGCLCC

42

35.7%

NZ_JACCCA010000013.1and 2 others:

NZ_JACHCM010000004.1 Lanthipeptides: HDE76_RS13915_lanthipeptideNZ_JACHCP010000005.1 Lanthipeptides: HDE80_RS12415_lanthipeptide

Lanthipeptides: HDE78_RS21145_lanthipeptide

5

GGTSCSYAGGCLCC

18

G T+CS AGGC CC

15

GSTTCSVAGGCACC

28

28.6%

NZ_NDXL01000001.1and 1 other:

NZ_NDXL01000001.1 Lanthipeptides: B7024_RS14560_lanthipeptide

Lanthipeptides: B7024_RS14560_lanthipeptide

6

GTSCSYAGGC--LCC

18

GTSC++ GC +CC

14

GTSCAFTNGCNPMCC

28

28.0%

NZ_JACHIP010000018.1:

Lanthipeptides: allorf_47599_47721_lanthipeptide

4

LGGTSCSYAGGCLC

17

LG T +AG CL

12

LGQTRRRHAGLCLV

25

21.6%

NZ_CP048405.1:

Lanthipeptides: GXW83_RS14070_lanthipeptide

6

GTSCSYAGGCLCC

18

GT+CSY+ G CC

25

GTACSYSAGRECC

37

15.2%

NZ_QFWW01000008.1:

Lanthipeptides: DI276_RS14925_lanthipeptide

6

GTSCSYAGGCLCC

18

GT C+Y+ G CC

34

GTVCTYSQGRQCC

46

allorf_202516_202659

Leader:

MPFHPCILGLVRTHAA

Core:

LSAAVRVRFAGGGEAACVSGRKRPDRQPSRW

Core with dehydrations:

LDhaAAVRVRFAGGGEAACysVDhaGRKRPDRQPDhaRW

antiSMASH-DB 4.0 matches for allorf_202516_202659

100.0%

Lanthipeptides: allorf_202516_202659_lanthipeptide

1

LSAAVRVRFAGGGEAACVSGRKRPDRQPSRW

31

LSAAVRVRFAGGGEAACVSGRKRPDRQPSRW

1

LSAAVRVRFAGGGEAACVSGRKRPDRQPSRW

31

51.9%

NZ_CP013140.1and 2 others:

NZ_CP067396.1 Lanthipeptides: allorf_5288223_5288408_lanthipeptideNZ_CP110813.1 Lanthipeptides: allorf_5600688_5600903_lanthipeptide

Lanthipeptides: allorf_0605449_0605655_lanthipeptide

1

LSAAVRVRFAGGGEAACVSGRKRPDRQPSR

30

LSAAVRVRFAGGGEAACVSGRKR DR+ SR

1

LSAAVRVRFAGGGEAACVSGRKRRDRESSR

30

29.3%

Lanthipeptides: allorf_3544541_3544756_lanthipeptide

2

SAAVRVRFAGGGEAACVSGRKRPDRQPSR

30

S +R R AG EA R+R D QP++

8

SQRMRRRGAGLREAGSSPMRRRNDAQPAK

36

with 5 more sequence matches

25.8%

NZ_KQ948355.1:

Lanthipeptides: AQJ11_RS50945_lanthipeptide

16

ACVSGRKRPDRQPSR

30

AC G RP R P R

6

ACAGGIARPFRTPPR

20

23.3%

NZ_CP012673.1:

Lanthipeptides: allorf_09528107_09528304_lanthipeptide

7

VRFAGGGEAACVSGRKRPDRQ

27

V A G AAC G++RP Q

22

VEDAKGHAAACGCGQRRPQIQ

42

22.6%

NZ_JAPTYM010000001.1:

Lanthipeptides: allorf_00315602_00315700_lanthipeptide

9

FAGGGEAACVSGRKRP

24

F GE C + R RP

1

FRQRGEVPCQTPRTRP

16

19.4%

NZ_CP047218.1and 1 other:

NZ_CP053021.1 Lassopeptides: HH800_RS08170_lassopeptide

Lassopeptides: GS397_RS04955_lassopeptide

19

SGRKRPDRQPS

29

SG PD+QP+

5

SGADNPDQQPN

15

14.3%

NZ_CP014241.1:

Lanthipeptides: allorf_1958314_1958487_lanthipeptide

21

RKRPDRQPS

29

RKRP+R P+

28

RKRPNRHPT

36

allorf_210339_210431

Leader:

MVRQRGARAGSQRGG

Core:

VSITCPGRVSVVRCG

Core with dehydrations:

VDhaIDhbCysPGRVDhaVVRCysG

antiSMASH-DB 4.0 matches for allorf_210339_210431

100.0%

Lanthipeptides: allorf_210339_210431_lanthipeptide

1

VSITCPGRVSVVRCG

15

VSITCPGRVSVVRCG

1

VSITCPGRVSVVRCG

15

86.7%

BGC0001229.4 (SBI_06989):

Lanthipeptides: allorf_0613381_0613473_lanthipeptide

1

VSITCPGRVSVVR

13

VSITCPGRVSVVR

1

VSITCPGRVSVVR

13

80.0%

NZ_CP067396.1and 1 other:

NZ_CP110813.1 Lanthipeptides: allorf_5592887_5593051_lanthipeptide

Lanthipeptides: allorf_5280413_5280577_lanthipeptide

1

VSITCPGRVSVVR

13

VSITCPGRVS+VR

1

VSITCPGRVSIVR

13

with 8 more sequence matches

33.3%

NZ_AP024745.1:

Lanthipeptides: allorf_07466148_07466240_lanthipeptide

3

ITCPGRVSVV

12

I CP VS++

1

IKCPSHVSII

10

23.1%

NZ_CP092497.1:

Lanthipeptides: MJQ72_RS19045_lanthipeptide

4

TCPGRVSVVRCG

15

+CP +SV CG

4

SCPSGLSVAACG

15

21.4%

NZ_BNAU01000004.1and 4 others:

NZ_CP091196.1 Lanthipeptides: L1857_RS00175_lanthipeptideNZ_FWXV01000003.1 Lanthipeptides: B9C43_RS55710_lanthipeptideNZ_KB903814.1 Lanthipeptides: A3G1_RS40055_lanthipeptideNZ_SGWQ01000004.1 Lanthipeptides: EV193_RS13500_lanthipeptide

Lanthipeptides: IE290_RS20710_lanthipeptide

4

TCPGRVSVVRCG

15

+CP +SV CG

6

SCPSNLSVTLCG

17

18.5%

NZ_FNAD01000001.1:

Lanthipeptides: BLQ63_RS02575_lanthipeptide

4

TCPGRVSVVRCG

15

+CP +S CG

5

SCPSNLSAALCG

16

17.9%

NZ_JACHGT010000003.1:

Lanthipeptides: HNR73_RS06650_lanthipeptide

4

TCPGRVSVVRCG

15

+CP +S + CG

6

SCPSNLSALLCG

17

17.1%

NC_019757.1:

Lanthipeptides: allorf_3740866_3741030_lanthipeptide

3

ITCPGRVSVVRCG

15

I C + VRCG

14

INCKSKFLFVRCG

26

16.7%

NZ_JAAATY010000001.1:

Lanthipeptides: GC106_RS02675_lanthipeptide

4

TCPGRVSVVRCG

15

+CP +S+ CG

8

SCPSNLSLTLCG

19

16.1%

NZ_FNGF01000002.1and 4 others:

NZ_JAGFNP010000002.1 Lanthipeptides: J5V16_RS05050_lanthipeptideNZ_JAPZVR010000008.1 Lanthipeptides: O2L52_RS16615_lanthipeptideNZ_RSEB01000004.1 Lanthipeptides: EIW28_RS16895_lanthipeptideNZ_WIAO01000001.1 Lanthipeptides: GFD30_RS00960_lanthipeptide

Lanthipeptides: BLS99_RS06065_lanthipeptide

4

TCPGRVSVVRCG

15

+CP +S CG

9

SCPSNLSATLCG

20

MIBiG 4.0 matches for allorf_210339_210431

10.8%

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

5

CPGRVSVVRC

14

CP RC

28

CPTTKCTSRC

37

allorf_211034_211114

Leader:

MDARARHGA

Core:

GCPLWRGPAQGAGPSGS

Core with dehydrations:

GCysPLWRGPAQGAGPDhaGDha

antiSMASH-DB 4.0 matches for allorf_211034_211114

100.0%

Lanthipeptides: allorf_211034_211114_lanthipeptide

1

GCPLWRGPAQGAGPSGS

17

GCPLWRGPAQGAGPSGS

1

GCPLWRGPAQGAGPSGS

17

47.1%

NZ_VEHK01000001.1:

Lanthipeptides: allorf_0068932_0069009_lanthipeptide

3

PLWRGPAQGAGP

14

PL RGP G GP

5

PLARGPCHGRGP

16

11.3%

NZ_JAIQCM010000003.1:

Lanthipeptides: K9V56_RS08295_lanthipeptide

5

WRGPAQGAGPSG

16

WR A G+ P+G

5

WRSQADGSNPAG

16

allorf_214830_215027

Leader:

MKRESLPAA

Core:

ACLQRSDPGMAFAAPAGLDDARARSGLRRSAPPDAACIRCARARTAVNWRARQTIA

Core with dehydrations:

ACysLQRDhaDPGMAFAAPAGLDDARARDhaGLRRDhaAPPDAACysIRCysARARDhbAVNWRARQDhbIA

antiSMASH-DB 4.0 matches for allorf_214830_215027

100.0%

BGC0002144.3 (RCCGEPOP_RS37510):

Lanthipeptides: allorf_214830_215027_lanthipeptide

1

ACLQRSDPGMAFAAPAGLDDARARSGLRRSAPPDAACIRCARARTAVNWRARQTIA

56

ACLQRSDPGMAFAAPAGLDDARARSGLRRSAPPDAACIRCARARTAVNWRARQTIA

1

ACLQRSDPGMAFAAPAGLDDARARSGLRRSAPPDAACIRCARARTAVNWRARQTIA

56

23.2%

NZ_FNAJ01000002.1:

Lanthipeptides: allorf_780201_780386_lanthipeptide

16

AGLDDARARS-----GLRRSAPPDAACIR

39

GL+ ARARS G R P D A +R

7

GGLEQARARSCPSAHGAVRPVPFDTAPVR

35

MIBiG 4.0 matches for allorf_214830_215027

7.1%

LAP: phazolicin

35

AACIRC

40

A C RC

1

ATCARC

6

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.56 out of 23.79

Confidence: medium

Additional weak hits (11):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.37 out of 30.22

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.38 out of 29.38

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.92 out of 26.33

Confidence: weak

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 17.99 out of 25.31

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.02 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.05 out of 27.78

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.55 out of 18.57

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.16 out of 31.73

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.58 out of 20.01

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 17.85 out of 25.88

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

BLV01_RS12410 - Class II

Cleavage pHMM score: -3.30

RODEO score: 0

Monoisotopic mass: 1582.6 Da

Molecular weight: 1583.8 Da

Number of bridges: 4

allorf_202516_202659 - Class II

Cleavage pHMM score: -5.90

RODEO score: 0

Monoisotopic mass: 3285.8 Da

Molecular weight: 3287.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3305.7 Da N = 1; 3323.7 Da N = 2

allorf_210339_210431 - Class II

Cleavage pHMM score: -6.70

RODEO score: 0

Monoisotopic mass: 1477.8 Da

Molecular weight: 1478.8 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1496.8 Da N = 1

allorf_211034_211114 - Class II

Cleavage pHMM score: -5.30

RODEO score: 0

Monoisotopic mass: 1560.7 Da

Molecular weight: 1561.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 1579.7 Da N = 1

allorf_214830_215027 - Class II

Cleavage pHMM score: -3.20

RODEO score: 0

Monoisotopic mass: 5845.0 Da

Molecular weight: 5848.6 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 5866.7 Da N = 1; 5884.7 Da N = 2

Predicted core structure(s)

For candidate cluster 6, location 169627 - 224436:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala) + (Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 7, location 169627 - 221008:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala) + (Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS12345: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (489..882): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSVSFDIALFELQAISTGEWNLYGPTETTVYSAL
Nearest Stachelhaus code(s):: Ala DILQITLVYK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLV01_RS12350: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (509..899): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDIALLELQALCGGEWNMYGPTETTVWSTL
Nearest Stachelhaus code(s):: Gly DILQLGMVWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLV01_RS12355: X

AMP-binding (528..931): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGFPFDASVGDPDAMIGGESNHYGPTETSVCAAM
Nearest Stachelhaus code(s):: CysA DATKMGHVGK (50% 8Å match)
Stachelhaus code match:: 70% (weak)

BLV01_RS12420: X

AMP-binding (32..464): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NQVPFALTCNCFTGFGGGTVEGYGLTESSPVAVV
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_FNPT01000017.1 - Region 1 - NRPS

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Location: 20,278 - 64,309 nt. (total: 44,032 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase glycosyltransferase sulfotransferase

BLV01_RS19700: aminotransferase

smcogs: SMCOG1019: aminotransferase

BLV01_RS19705: glutathione S-transferase

smcogs: SMCOG1193: glutathione S-transferase

BLV01_RS19760: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

Ligases: ligase

BLV01_RS19755: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001240.4	0.63	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002075.3	0.74	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001240.4	0.63	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 21.40 out of 23.05

Confidence: strong

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 22.73 out of 29.25

Confidence: medium

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 26.54 out of 32.74

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.24 out of 19.26

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.24 out of 19.26

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.75 out of 23.05

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.18 out of 23.79

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.40 out of 19.26

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.98 out of 27.78

Confidence: medium

Additional weak hits (29):

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.40 out of 29.51

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.15 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.49 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.28 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.36 out of 26.06

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.82 out of 30.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.99 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.53 out of 29.38

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.77 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.75 out of 26.06

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.53 out of 20.97

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.73 out of 29.25

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.57 out of 28.14

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.08 out of 31.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.09 out of 30.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.60 out of 29.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.80 out of 30.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.17 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.96 out of 33.14

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.59 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.78 out of 32.93

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.60 out of 30.98

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.25 out of 36.53

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.61 out of 26.06

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.79 out of 20.01

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.32 out of 26.06

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 19.95 out of 30.98

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.61 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.65 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 20277 - 64309:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS19755: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (462..863): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FAMTFDIAGLELQALVGGETNLYGPTETTVWSTA
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
FuR variant 2	Ferric Uptake Regulator
IolR	Regulator of the inositol catabolism
SypG	Regulator of biofilm formation and host colonization

NZ_FNPT01000004.1 - Region 1 - lanthipeptide-class-ii

Download region SVG

Location: 342,927 - 365,782 nt. (total: 22,856 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

BLV01_RS09905: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002704.2	0.46	ribosomal	archalan β	Halorussus salinus
BGC0002703.2	0.44	ribosomal	archalan α	Halorussus salinus
BGC0000554.4	0.43	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000528.5	0.43	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000523.3	0.39	ribosomal	lactocin S	Lactobacillus sakei
BGC0002346.3	0.38	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000517.4	0.38	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000527.5	0.36	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0001311.5	0.34	ribosomal	flavecins	Ruminococcus flavefaciens FD-1
BGC0002330.2	0.32	ribosomal	landornamide A	Kamptonema sp. PCC 6506

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002704.2	0.46	ribosomal	archalan β	Halorussus salinus
BGC0002703.2	0.44	ribosomal	archalan α	Halorussus salinus
BGC0000554.4	0.43	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000528.5	0.43	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000523.3	0.39	ribosomal	lactocin S	Lactobacillus sakei
BGC0002346.3	0.38	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000517.4	0.38	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000527.5	0.36	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0001311.5	0.34	ribosomal	flavecins	Ruminococcus flavefaciens FD-1
BGC0002330.2	0.32	ribosomal	landornamide A	Kamptonema sp. PCC 6506

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BLV01_RS09885

allorf_344999_345145

Leader:

MPNWVADSARIVHLRNARLYFG

Core:

CKSEAMRTRSVVMVSGATRLGGHPAT

Core with dehydrations:

CysKDhaEAMRDhbRDhaVVMVDhaGADhbRLGGHPADhb

antiSMASH-DB 4.0 matches for allorf_344999_345145

100.0%

Lanthipeptides: allorf_344999_345145_lanthipeptide

1

CKSEAMRTRSVVMVSGATRLGGHPAT

26

CKSEAMRTRSVVMVSGATRLGGHPAT

1

CKSEAMRTRSVVMVSGATRLGGHPAT

26

24.1%

NZ_PKMC01000001.1:

Lanthipeptides: allorf_0147058_0147204_lanthipeptide

2

KSEAMRTRSVVMVSGATRLGG

22

EA+ T ++ +G T GG

1

DDEALITSFILCTAGCTSKGG

21

15.4%

NZ_CP029710.1:

Lanthipeptides: DMB37_RS40355_lanthipeptide

17

ATRLGGHPAT

26

AT++GG+P T

1

ATQVGGNPTT

10

allorf_345034_345240

Leader:

MLRNEIAA

Core:

RRGGEVRRAGGDVRPLPLLTAKGPHVAGCPPRRVAPDTITTERVRIASDLHPKYSRAFLK

Core with dehydrations:

RRGGEVRRAGGDVRPLPLLDhbAKGPHVAGCysPPRRVAPDDhbIDhbDhbERVRIADhaDLHPKYDhaRAFLK

antiSMASH-DB 4.0 matches for allorf_345034_345240

100.0%

Lanthipeptides: allorf_345034_345240_lanthipeptide

1

RRGGEVRRAGGDVRPLPLLTAKGPHVAGCPPRRVAPDTITTERVRIASDLHPKYSRAFLK

60

RRGGEVRRAGGDVRPLPLLTAKGPHVAGCPPRRVAPDTITTERVRIASDLHPKYSRAFLK

1

RRGGEVRRAGGDVRPLPLLTAKGPHVAGCPPRRVAPDTITTERVRIASDLHPKYSRAFLK

60

23.4%

NZ_CP011152.1and 3 others:

NZ_CP011152.1 Lanthipeptides: WR47_RS28815_lanthipeptideNZ_CP011152.1 Lanthipeptides: WR47_RS28820_lanthipeptideNZ_CP011152.1 Lanthipeptides: WR47_RS28815_lanthipeptide

Lanthipeptides: WR47_RS28820_lanthipeptide

2

RGGEVRRA--GGDVRP------LPLLTAKGPHVAGCPPRRVAPDTITTE

42

+G E+ A GDV+ + LTAK P+++ + TIT E

10

QGNELLNAVGAGDVQANTNTIVVTQLTAKSPYISNTLGNKGHACTITKE

58

15.0%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: IE314_RS15870_lanthipeptide

Lanthipeptides: IE314_RS15870_lanthipeptide

4

GEVRRAGGDVRPLPLLTAKGPHVAGCPPRRV

34

GE GG P+ + T+ A CP +

10

GEDVAHGGTTSPVCIATSVAVSTAFCPTTKC

40

with 1 more sequence matches

11.7%

NZ_JAQDSE010000019.1:

Lanthipeptides: PG965_RS14705_lanthipeptide

18

LLTAKGPHVAGCPPRRVAPDTITTE

42

T G + CP +P +TT

11

CFTFDGSNTCNCPSTDCSPCDMTTS

35

allorf_355996_356196

Leader:

MVSGHRSESAAHSSALMCSNGA

Core:

RPLFDTSRTSKRFCNLFKCSLMVVSHSCPADRPPDTTLRFRRRT

Core with dehydrations:

RPLFDDhbDhaRDhbDhaKRFCysNLFKCysDhaLMVVDhaHDhaCysPADRPPDDhbDhbLRFRRRDhb

antiSMASH-DB 4.0 matches for allorf_355996_356196

100.0%

BGC0001229.4 (SBI_06989):

Lanthipeptides: allorf_355996_356196_lanthipeptide

1

RPLFDTSRTSKRFCNLFKCSLMVVSHSCPADRPPDTTLRFRRRT

44

RPLFDTSRTSKRFCNLFKCSLMVVSHSCPADRPPDTTLRFRRRT

1

RPLFDTSRTSKRFCNLFKCSLMVVSHSCPADRPPDTTLRFRRRT

44

22.7%

NC_017803.1:

Lanthipeptides: ACPL_RS19820_lanthipeptide

3

LFDTSRTSKRFCNLFKCSLMVVSHSCPADRPPD

35

+ DT RT R + + + CP PPD

8

VADTGRTEARAAEVVTAFGIASTTCCP---PPD

37

20.5%

NZ_ATDF02000020.1and 1 other:

NZ_KV827785.1 Sactipeptides: HMPREF2580_RS10900_sactipeptide

Sactipeptides: M462_RS08950_sactipeptide

18

KCSLMVVSHSCPADRP-PD

35

CS V +C AD P PD

1

GCSACAVGAACLADGPIPD

19

with 7 more sequence matches

20.5%

NZ_AXVJ01000055.1:

Lanthipeptides: K339_RS36965_lanthipeptide

9

TSKRFCNLFKCSLMVVSHSC

28

TSK +C+ CS S SC

12

TSKHYCHSDLCSFTCASWSC

31

20.5%

NZ_CP031030.1:

Lanthipeptides: DTQ70_RS30930_lanthipeptide

9

TSKRFCNLFKCSLMVVSHSC

28

TSK +C+ CS S SC

12

TSKHYCHSDLCSFTCPSWSC

31

20.5%

NZ_NWMU01000024.1and 1 other:

NZ_PNGD01000001.1 Lanthipeptides: allorf_038888_039007_lanthipeptide

Lanthipeptides: allorf_25415_25534_lanthipeptide

14

CNLFKCSLMVVSHSCPADRPPDTTLRF

40

C++F S +V + S + R P T +F

1

CSVFASSDIVSTISQKSYRQPTKTFQF

27

20.5%

NZ_PIZD01000012.1:

Sactipeptides: allorf_25390_25524_sactipeptide

18

KCSLMVVSHSCPADRP-PDTTL

38

+CS V C AD P PD +

2

ECSFCAVGAGCLADGPFPDAEV

23

18.2%

NC_022584.1and 2 others:

NZ_CP047883.1 Sactipeptides: M594_RS05585_sactipeptideNZ_KV802690.1 Sactipeptides: HMPREF2764_RS03300_sactipeptide

Sactipeptides: N596_RS03165_sactipeptide

18

KCSLMVVSHSCPADRP-PD

35

CS+ + C AD P PD

1

GCSVCAIGAVCLADGPIPD

19

18.2%

NZ_BBZK01000004.1:

Lanthipeptides: allorf_248358_248516_lanthipeptide

17

FKCSLMVVSHSCPADRPP

34

+ +L SHSC A R P

21

YLMALSATSHSCSAVRRP

38

18.2%

NZ_CP094808.1and 3 others:

NZ_CP094809.1 Sactipeptides: MUA95_RS01320_sactipeptideNZ_CP094813.1 Sactipeptides: MUA84_RS01300_sactipeptideNZ_VKCY01000004.1 Sactipeptides: FNK43_RS05155_sactipeptide

Sactipeptides: MUA11_RS01375_sactipeptide

18

KCSLMVVSHSCPADRP-PD

35

CS + +C AD P PD

1

GCSACAIGAACLADGPIPD

19

MIBiG 4.0 matches for allorf_355996_356196

11.4%

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

10

SKRFCNLFKCS

20

S FC KC+

24

SAAFCPTTKCT

34

allorf_356335_356562

Leader:

MEPQYDRPAAGQARREAERIVREATFNAILNQAAAG

Core:

RIHSWDFRARRWHAPDLPRCRRMRWATSNLRSTSALTPA

Core with dehydrations:

RIHDhaWDFRARRWHAPDLPRCysRRMRWADhbDhaNLRDhaDhbDhaALDhbPA

antiSMASH-DB 4.0 matches for allorf_356335_356562

100.0%

Lanthipeptides: allorf_356335_356562_lanthipeptide

1

RIHSWDFRARRWHAPDLPRCRRMRWATSNLRSTSALTPA

39

RIHSWDFRARRWHAPDLPRCRRMRWATSNLRSTSALTPA

1

RIHSWDFRARRWHAPDLPRCRRMRWATSNLRSTSALTPA

39

17.9%

NZ_LMHM01000013.1:

Lanthipeptides: allorf_293905_294090_lanthipeptide

9

ARRWHAPDLPRCR

21

+R APD+ RCR

26

CKRGRAPDVLRCR

38

15.4%

NZ_CP064030.1:

Lanthipeptides: allorf_1946039_1946143_lanthipeptide

11

RWHAPDLPRCRR

22

RW + LP CR+

2

RWQSKALPGCRQ

13

Lanthipeptide(s) for BLV01_RS09905

allorf_344999_345145

Leader:

MPNWVADSARIVHLRNARLYFG

Core:

CKSEAMRTRSVVMVSGATRLGGHPAT

Core with dehydrations:

CysKDhaEAMRDhbRDhaVVMVDhaGADhbRLGGHPADhb

antiSMASH-DB 4.0 matches for allorf_344999_345145

100.0%

Lanthipeptides: allorf_344999_345145_lanthipeptide

1

CKSEAMRTRSVVMVSGATRLGGHPAT

26

CKSEAMRTRSVVMVSGATRLGGHPAT

1

CKSEAMRTRSVVMVSGATRLGGHPAT

26

24.1%

NZ_PKMC01000001.1:

Lanthipeptides: allorf_0147058_0147204_lanthipeptide

2

KSEAMRTRSVVMVSGATRLGG

22

EA+ T ++ +G T GG

1

DDEALITSFILCTAGCTSKGG

21

15.4%

NZ_CP029710.1:

Lanthipeptides: DMB37_RS40355_lanthipeptide

17

ATRLGGHPAT

26

AT++GG+P T

1

ATQVGGNPTT

10

allorf_345034_345240

Leader:

MLRNEIAA

Core:

RRGGEVRRAGGDVRPLPLLTAKGPHVAGCPPRRVAPDTITTERVRIASDLHPKYSRAFLK

Core with dehydrations:

RRGGEVRRAGGDVRPLPLLDhbAKGPHVAGCysPPRRVAPDDhbIDhbDhbERVRIADhaDLHPKYDhaRAFLK

antiSMASH-DB 4.0 matches for allorf_345034_345240

100.0%

Lanthipeptides: allorf_345034_345240_lanthipeptide

1

RRGGEVRRAGGDVRPLPLLTAKGPHVAGCPPRRVAPDTITTERVRIASDLHPKYSRAFLK

60

RRGGEVRRAGGDVRPLPLLTAKGPHVAGCPPRRVAPDTITTERVRIASDLHPKYSRAFLK

1

RRGGEVRRAGGDVRPLPLLTAKGPHVAGCPPRRVAPDTITTERVRIASDLHPKYSRAFLK

60

23.4%

NZ_CP011152.1and 3 others:

NZ_CP011152.1 Lanthipeptides: WR47_RS28815_lanthipeptideNZ_CP011152.1 Lanthipeptides: WR47_RS28820_lanthipeptideNZ_CP011152.1 Lanthipeptides: WR47_RS28815_lanthipeptide

Lanthipeptides: WR47_RS28820_lanthipeptide

2

RGGEVRRA--GGDVRP------LPLLTAKGPHVAGCPPRRVAPDTITTE

42

+G E+ A GDV+ + LTAK P+++ + TIT E

10

QGNELLNAVGAGDVQANTNTIVVTQLTAKSPYISNTLGNKGHACTITKE

58

15.0%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: IE314_RS15870_lanthipeptide

Lanthipeptides: IE314_RS15870_lanthipeptide

4

GEVRRAGGDVRPLPLLTAKGPHVAGCPPRRV

34

GE GG P+ + T+ A CP +

10

GEDVAHGGTTSPVCIATSVAVSTAFCPTTKC

40

with 1 more sequence matches

11.7%

NZ_JAQDSE010000019.1:

Lanthipeptides: PG965_RS14705_lanthipeptide

18

LLTAKGPHVAGCPPRRVAPDTITTE

42

T G + CP +P +TT

11

CFTFDGSNTCNCPSTDCSPCDMTTS

35

allorf_355996_356196

Leader:

MVSGHRSESAAHSSALMCSNGA

Core:

RPLFDTSRTSKRFCNLFKCSLMVVSHSCPADRPPDTTLRFRRRT

Core with dehydrations:

RPLFDDhbDhaRDhbDhaKRFCysNLFKCysDhaLMVVDhaHDhaCysPADRPPDDhbDhbLRFRRRDhb

antiSMASH-DB 4.0 matches for allorf_355996_356196

100.0%

BGC0001229.4 (SBI_06989):

Lanthipeptides: allorf_355996_356196_lanthipeptide

1

RPLFDTSRTSKRFCNLFKCSLMVVSHSCPADRPPDTTLRFRRRT

44

RPLFDTSRTSKRFCNLFKCSLMVVSHSCPADRPPDTTLRFRRRT

1

RPLFDTSRTSKRFCNLFKCSLMVVSHSCPADRPPDTTLRFRRRT

44

22.7%

NC_017803.1:

Lanthipeptides: ACPL_RS19820_lanthipeptide

3

LFDTSRTSKRFCNLFKCSLMVVSHSCPADRPPD

35

+ DT RT R + + + CP PPD

8

VADTGRTEARAAEVVTAFGIASTTCCP---PPD

37

20.5%

NZ_ATDF02000020.1and 1 other:

NZ_KV827785.1 Sactipeptides: HMPREF2580_RS10900_sactipeptide

Sactipeptides: M462_RS08950_sactipeptide

18

KCSLMVVSHSCPADRP-PD

35

CS V +C AD P PD

1

GCSACAVGAACLADGPIPD

19

with 7 more sequence matches

20.5%

NZ_AXVJ01000055.1:

Lanthipeptides: K339_RS36965_lanthipeptide

9

TSKRFCNLFKCSLMVVSHSC

28

TSK +C+ CS S SC

12

TSKHYCHSDLCSFTCASWSC

31

20.5%

NZ_CP031030.1:

Lanthipeptides: DTQ70_RS30930_lanthipeptide

9

TSKRFCNLFKCSLMVVSHSC

28

TSK +C+ CS S SC

12

TSKHYCHSDLCSFTCPSWSC

31

20.5%

NZ_NWMU01000024.1and 1 other:

NZ_PNGD01000001.1 Lanthipeptides: allorf_038888_039007_lanthipeptide

Lanthipeptides: allorf_25415_25534_lanthipeptide

14

CNLFKCSLMVVSHSCPADRPPDTTLRF

40

C++F S +V + S + R P T +F

1

CSVFASSDIVSTISQKSYRQPTKTFQF

27

20.5%

NZ_PIZD01000012.1:

Sactipeptides: allorf_25390_25524_sactipeptide

18

KCSLMVVSHSCPADRP-PDTTL

38

+CS V C AD P PD +

2

ECSFCAVGAGCLADGPFPDAEV

23

18.2%

NC_022584.1and 2 others:

NZ_CP047883.1 Sactipeptides: M594_RS05585_sactipeptideNZ_KV802690.1 Sactipeptides: HMPREF2764_RS03300_sactipeptide

Sactipeptides: N596_RS03165_sactipeptide

18

KCSLMVVSHSCPADRP-PD

35

CS+ + C AD P PD

1

GCSVCAIGAVCLADGPIPD

19

18.2%

NZ_BBZK01000004.1:

Lanthipeptides: allorf_248358_248516_lanthipeptide

17

FKCSLMVVSHSCPADRPP

34

+ +L SHSC A R P

21

YLMALSATSHSCSAVRRP

38

18.2%

NZ_CP094808.1and 3 others:

NZ_CP094809.1 Sactipeptides: MUA95_RS01320_sactipeptideNZ_CP094813.1 Sactipeptides: MUA84_RS01300_sactipeptideNZ_VKCY01000004.1 Sactipeptides: FNK43_RS05155_sactipeptide

Sactipeptides: MUA11_RS01375_sactipeptide

18

KCSLMVVSHSCPADRP-PD

35

CS + +C AD P PD

1

GCSACAIGAACLADGPIPD

19

MIBiG 4.0 matches for allorf_355996_356196

11.4%

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

10

SKRFCNLFKCS

20

S FC KC+

24

SAAFCPTTKCT

34

allorf_356335_356562

Leader:

MEPQYDRPAAGQARREAERIVREATFNAILNQAAAG

Core:

RIHSWDFRARRWHAPDLPRCRRMRWATSNLRSTSALTPA

Core with dehydrations:

RIHDhaWDFRARRWHAPDLPRCysRRMRWADhbDhaNLRDhaDhbDhaALDhbPA

antiSMASH-DB 4.0 matches for allorf_356335_356562

100.0%

Lanthipeptides: allorf_356335_356562_lanthipeptide

1

RIHSWDFRARRWHAPDLPRCRRMRWATSNLRSTSALTPA

39

RIHSWDFRARRWHAPDLPRCRRMRWATSNLRSTSALTPA

1

RIHSWDFRARRWHAPDLPRCRRMRWATSNLRSTSALTPA

39

17.9%

NZ_LMHM01000013.1:

Lanthipeptides: allorf_293905_294090_lanthipeptide

9

ARRWHAPDLPRCR

21

+R APD+ RCR

26

CKRGRAPDVLRCR

38

15.4%

NZ_CP064030.1:

Lanthipeptides: allorf_1946039_1946143_lanthipeptide

11

RWHAPDLPRCRR

22

RW + LP CR+

2

RWQSKALPGCRQ

13

allorf_361083_361250

Leader:

MVSRRIANLDLHDARGA

Core:

VYANMRDFVQIWINRPGACGERGALPRARSLGEGIGHG

Core with dehydrations:

VYANMRDFVQIWINRPGACysGERGALPRARDhaLGEGIGHG

antiSMASH-DB 4.0 matches for allorf_361083_361250

100.0%

BGC0000517.4 (BAB04173.1):

Lanthipeptides: allorf_361083_361250_lanthipeptide

1

VYANMRDFVQIWINRPGACGERGALPRARSLGEGIGHG

38

VYANMRDFVQIWINRPGACGERGALPRARSLGEGIGHG

1

VYANMRDFVQIWINRPGACGERGALPRARSLGEGIGHG

38

15.8%

NZ_BMUQ01000012.1:

Lanthipeptides: allorf_026367_026534_lanthipeptide

26

PRARSLGEGI

35

P ++SLGEG+

2

PCSKSLGEGV

11

15.8%

NZ_JAQFWW010000005.1:

Lanthipeptides: PDT38_RS15180_lanthipeptide

24

ALPRARSLGEGIGHG

38

++ +ARS+G G +G

7

SIAKARSMGLGTNYG

21

with 1 more sequence matches

12.3%

NZ_AP014940.1:

Lanthipeptides: LEN_RS12920_lanthipeptide

1

VYANMRDFVQIWINRPGACGERGALPRAR

29

+Y N + + +P +CG+ G P +

19

IYYNDAGTAVVGVRQPASCGDTGYGPTTK

47

MIBiG 4.0 matches for allorf_361083_361250

13.2%

Lanthipeptide: haloduracin β or haloduracin α

12

WINRPGACGERGA

24

W N G +GA

3

WYNISCRLGNKGA

15

11.8%

BGC0002120.3 (NQE92413.1):

Lanthipeptide: nocathioamide A

14

NRPGA--CGERGALP

26

+RP A C +LP

33

SRPAAPACHTLSSLP

47

allorf_361247_361429

Leader:

MRNSDFRSPLRAETGA

Core:

PVASGQPRRSMCCAERGGDRATLEIATENCDEIEQSGPAAQPRG

Core with dehydrations:

PVADhaGQPRRDhaMCysCysAERGGDRADhbLEIADhbENCysDEIEQDhaGPAAQPRG

antiSMASH-DB 4.0 matches for allorf_361247_361429

100.0%

Lanthipeptides: allorf_361247_361429_lanthipeptide

1

PVASGQPRRSMCCAERGGDRATLEIATENCDEIEQSGPAAQPRG

44

PVASGQPRRSMCCAERGGDRATLEIATENCDEIEQSGPAAQPRG

1

PVASGQPRRSMCCAERGGDRATLEIATENCDEIEQSGPAAQPRG

44

22.7%

NZ_SNZH01000011.1:

Lanthipeptides: allorf_232150_232323_lanthipeptide

3

ASGQPRRSMCCAE--RGGDRATLEI

25

A P S+ C RGGD AT+ +

11

AGAAPLPSVMCGPGGRGGDWATMRV

35

18.2%

NZ_CP077072.1:

Lanthipeptides: allorf_2268889_2269032_lanthipeptide

30

CDEIEQSGPAAQPRG

44

C I Q GP PRG

13

CHRIHQDGPVHLPRG

27

with 2 more sequence matches

15.9%

NZ_JAGGRJ010000031.1:

Lanthipeptides: allorf_020847_020975_lanthipeptide

6

QPRRSMCCAERG

17

+PRR++C A RG

5

RPRRAICDAARG

16

13.8%

NZ_CP017169.1:

Lanthipeptides: allorf_8034042_8034278_lanthipeptide

7

PRRSMCCAERGGDRATLEIATE

28

PR S C G RA+ ++ E

11

PRLSTRCTRHWGQRASAQVVRE

32

allorf_361277_361408

Leader:

MFDLVTIFGC

Core:

DFECGPIPAAFGATHAATGLSACDRRTRFCPQR

Core with dehydrations:

DFECysGPIPAAFGADhbHAADhbGLDhaACysDRRDhbRFCysPQR

antiSMASH-DB 4.0 matches for allorf_361277_361408

100.0%

Lanthipeptides: allorf_361277_361408_lanthipeptide

1

DFECGPIPAAFGATHAATGLSACDRRTRFCPQR

33

DFECGPIPAAFGATHAATGLSACDRRTRFCPQR

1

DFECGPIPAAFGATHAATGLSACDRRTRFCPQR

33

22.9%

NZ_CP120210.1:

Lanthipeptides: P3102_RS07330_lanthipeptide

7

IPAAFGATHAATGLSACDRR

26

IP + T TG +AC R

4

IPCSIPCTRICTGRTACCSR

23

20.5%

NZ_CP020327.1:

Lanthipeptides: allorf_1389413_1389616_lanthipeptide

21

SACDRRTRFCPQ

32

SACD TRF P

24

SACDSSTRFLPN

35

with 2 more sequence matches

15.6%

NZ_CP026035.1:

Lanthipeptides: C2H96_RS00305_lanthipeptide

20

LSACDRRTRFC

30

LSA R TR C

15

LSAVGRSTRLC

25

14.8%

NZ_CP050134.2:

Lanthipeptides: allorf_0180903_0181139_lanthipeptide

2

FECGPIPAAFGATHAATGLSACD

24

F P+P GA LS C+

31

FWAFPVPGPMGAAFLKLQLSTCE

53

allorf_365180_365317

Leader:

MAGRGCAVTGWRMRSGVARDAVSLVAG

Core:

CSSLVVGCVLSSRGMRLH

Core with dehydrations:

CysDhaDhaLVVGCysVLDhaDhaRGMRLH

antiSMASH-DB 4.0 matches for allorf_365180_365317

100.0%

Lanthipeptides: allorf_365180_365317_lanthipeptide

1

CSSLVVGCVLSSRGMRLH

18

CSSLVVGCVLSSRGMRLH

1

CSSLVVGCVLSSRGMRLH

18

15.4%

NZ_KV797630.1:

Lanthipeptides: allorf_070105_070269_lanthipeptide

4

LVVGCVLSSRGM

15

L+VGCV G+

22

LIVGCVFEVYGI

33

allorf_365333_365488

Leader:

MPAVVPAKAGIHFDFSVALALIEAA

Core:

SDDKPRSKASVRKRPGHFLCPKQQRK

Core with dehydrations:

DhaDDKPRDhaKADhaVRKRPGHFLCysPKQQRK

antiSMASH-DB 4.0 matches for allorf_365333_365488

100.0%

Lanthipeptides: allorf_365333_365488_lanthipeptide

1

SDDKPRSKASVRKRPGHFLCPKQQRK

26

SDDKPRSKASVRKRPGHFLCPKQQRK

1

SDDKPRSKASVRKRPGHFLCPKQQRK

26

30.8%

NZ_CP026498.1:

Lanthipeptides: allorf_00224338_00224481_lanthipeptide

7

SKASVRKRPGHFLCPKQQR

25

S+ VR+RPG C + R

5

SRGGVRRRPGEVPCERGAR

23

allorf_365518_365742

Leader:

MPSFRRKPESTRSCRCRCRCRCRCFRSSLFARTQPHVKHAFRSPGG

Core:

RAHGCARAPWGRMPLMARPRAPVSHSGS

Core with dehydrations:

RAHGCysARAPWGRMPLMARPRAPVDhaHDhaGDha

antiSMASH-DB 4.0 matches for allorf_365518_365742

100.0%

Lanthipeptides: allorf_365518_365742_lanthipeptide

1

RAHGCARAPWGRMPLMARPRAPVSHSGS

28

RAHGCARAPWGRMPLMARPRAPVSHSGS

1

RAHGCARAPWGRMPLMARPRAPVSHSGS

28

50.0%

BGC0002120.3 (NQE92413.1):

Lanthipeptides: allorf_0614108_0614290_lanthipeptide

1

RAHGCARAPWGRMPLMARPRAPVS

24

RAHGCARAPWGRMPLMARPRAP

1

RAHGCARAPWGRMPLMARPRAPFE

24

30.6%

NZ_FNON01000003.1:

Lanthipeptides: allorf_0337192_0337368_lanthipeptide

1

RAHGCARAPWGRMPLMARPRAPVSHSG

27

R GC +AP + R +AP HSG

4

RPLGCMKAPLIKP---ERMKAPFIHSG

27

with 1 more sequence matches

21.4%

NZ_AP014940.1and 7 others:

NZ_CP013140.1 Lanthipeptides: GLE_RS10670_lanthipeptideNZ_CP067395.1 Lanthipeptides: JHW38_RS03600_lanthipeptideNZ_CP067396.1 Lanthipeptides: JHW41_RS13260_lanthipeptideNZ_CP072597.1 Lanthipeptides: J5226_RS17490_lanthipeptideNZ_CP110813.1 Lanthipeptides: BV903_RS14555_lanthipeptideNZ_FNOG01000005.1 Lanthipeptides: BLU84_RS14520_lanthipeptideNZ_FNPT01000003.1 Lanthipeptides: BLV01_RS07955_lanthipeptide

Lanthipeptides: LEN_RS12910_lanthipeptide

20

RAPVSHSGS

28

RAP++HS S

1

RAPITHSTS

9

MIBiG 4.0 matches for allorf_365518_365742

11.8%

Lanthipeptide: nocathioamide A

17

ARPRAPVSHSGS

28

+RP AP H+ S

33

SRPAAPACHTLS

44

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

Additional weak hits (6):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.58 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.46 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.39 out of 29.38

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.88 out of 30.98

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.16 out of 33.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.15 out of 29.65

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

allorf_344999_345145 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 2593.4 Da

Molecular weight: 2595.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2613.0 Da N = 1; 2631.0 Da N = 2; 2649.1 Da N = 3; 2667.1 Da N = 4; 2685.1 Da N = 5

allorf_345034_345240 - Class II

Cleavage pHMM score: -4.50

RODEO score: 0

Monoisotopic mass: 6458.6 Da

Molecular weight: 6462.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 6480.5 Da N = 1; 6498.5 Da N = 2; 6516.5 Da N = 3; 6534.5 Da N = 4; 6552.5 Da N = 5

allorf_355996_356196 - Class II

Cleavage pHMM score: -4.30

RODEO score: 0

Monoisotopic mass: 5017.7 Da

Molecular weight: 5020.8 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 5038.8 Da N = 1; 5056.8 Da N = 2; 5074.9 Da N = 3; 5092.9 Da N = 4; 5110.9 Da N = 5; 5128.9 Da N = 6; 5146.9 Da N = 7

allorf_356335_356562 - Class II

Cleavage pHMM score: -4.00

RODEO score: 0

Monoisotopic mass: 4604.4 Da

Molecular weight: 4607.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4625.3 Da N = 1; 4643.3 Da N = 2; 4661.3 Da N = 3; 4679.3 Da N = 4; 4697.3 Da N = 5; 4715.4 Da N = 6

allorf_361083_361250 - Class II

Cleavage pHMM score: -4.50

RODEO score: 0

Monoisotopic mass: 4105.1 Da

Molecular weight: 4107.6 Da

Number of bridges: 1

allorf_361247_361429 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 4523.1 Da

Molecular weight: 4525.9 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 4544.0 Da N = 1; 4562.0 Da N = 2

allorf_361277_361408 - Class II

Cleavage pHMM score: -4.50

RODEO score: 0

Monoisotopic mass: 3449.7 Da

Molecular weight: 3451.9 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 3469.9 Da N = 1

allorf_365180_365317 - Class II

Cleavage pHMM score: -5.90

RODEO score: 0

Monoisotopic mass: 1831.0 Da

Molecular weight: 1832.2 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1850.2 Da N = 1; 1868.2 Da N = 2

allorf_365333_365488 - Class II

Cleavage pHMM score: -5.90

RODEO score: 0

Monoisotopic mass: 2994.7 Da

Molecular weight: 2996.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3014.5 Da N = 1; 3032.5 Da N = 2

allorf_365518_365742 - Class II

Cleavage pHMM score: -7.30

RODEO score: 0

Monoisotopic mass: 2957.5 Da

Molecular weight: 2959.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2977.5 Da N = 1; 2995.5 Da N = 2

allorf_344999_345145 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 2593.4 Da

Molecular weight: 2595.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2613.0 Da N = 1; 2631.0 Da N = 2; 2649.1 Da N = 3; 2667.1 Da N = 4; 2685.1 Da N = 5

allorf_345034_345240 - Class II

Cleavage pHMM score: -4.50

RODEO score: 0

Monoisotopic mass: 6458.6 Da

Molecular weight: 6462.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 6480.5 Da N = 1; 6498.5 Da N = 2; 6516.5 Da N = 3; 6534.5 Da N = 4; 6552.5 Da N = 5

allorf_355996_356196 - Class II

Cleavage pHMM score: -4.30

RODEO score: 0

Monoisotopic mass: 5017.7 Da

Molecular weight: 5020.8 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 5038.8 Da N = 1; 5056.8 Da N = 2; 5074.9 Da N = 3; 5092.9 Da N = 4; 5110.9 Da N = 5; 5128.9 Da N = 6; 5146.9 Da N = 7

allorf_356335_356562 - Class II

Cleavage pHMM score: -4.00

RODEO score: 0

Monoisotopic mass: 4604.4 Da

Molecular weight: 4607.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4625.3 Da N = 1; 4643.3 Da N = 2; 4661.3 Da N = 3; 4679.3 Da N = 4; 4697.3 Da N = 5; 4715.4 Da N = 6

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNPT01000003.1 - Region 1 - RiPP-like

Download region SVG

Location: 174,415 - 185,695 nt. (total: 11,281 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001657.4	0.27	NRPS	lysocin	Lysobacter sp. RH2180-5
BGC0001157.6	0.26	ribosomal	bottromycin A2	Streptomyces scabiei 87.22
BGC0000468.5	0.26	ribosomal	bottromycin A2	Streptomyces bottropensis DSM40262
BGC0000470.6	0.26	ribosomal	bottromycin D	Streptomyces sp. WMMB272
BGC0000469.6	0.24	ribosomal	bottromycin A2	Streptomyces sp. BC16019
BGC0001471.5	0.19	ribosomal	saalfelduracin	Amycolatopsis saalfeldensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001657.4	0.27	NRPS	lysocin	Lysobacter sp. RH2180-5
BGC0001157.6	0.26	ribosomal	bottromycin A2	Streptomyces scabiei 87.22
BGC0000468.5	0.26	ribosomal	bottromycin A2	Streptomyces bottropensis DSM40262
BGC0000470.6	0.26	ribosomal	bottromycin D	Streptomyces sp. WMMB272
BGC0000469.6	0.24	ribosomal	bottromycin A2	Streptomyces sp. BC16019
BGC0001471.5	0.19	ribosomal	saalfelduracin	Amycolatopsis saalfeldensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.07 out of 20.47

Confidence: medium

TFBS Finder

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNPT01000003.1 - Region 2 - lanthipeptide-class-ii

BGC0000535.4 (ADJ56352.1):

Download region SVG

Location: 232,549 - 255,410 nt. (total: 22,862 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLV01_RS07935: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: hydrolase

BLV01_RS07950: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

BLV01_RS07980: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: cyclase

BLV01_RS07960: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.57	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000528.5	0.48	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002704.2	0.46	ribosomal	archalan β	Halorussus salinus
BGC0000894.4	0.46	other (other)	citrinin	Monascus aurantiacus
BGC0000867.3	0.44	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0000503.5	0.44	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001579.4	0.43	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0000231.5	0.42	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002346.3	0.42	ribosomal	kyamicin	Saccharopolyspora sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.57	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000528.5	0.48	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002704.2	0.46	ribosomal	archalan β	Halorussus salinus
BGC0000894.4	0.46	other (other)	citrinin	Monascus aurantiacus
BGC0000867.3	0.44	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0000503.5	0.44	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001579.4	0.43	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0001576.3	0.43	ribosomal	divamide A	Prochloron didemni
BGC0000231.5	0.42	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0002346.3	0.42	ribosomal	kyamicin	Saccharopolyspora sp.

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BLV01_RS07960

BLV01_RS07955

Leader:

MQSNDLVSGWLTGAETVMGFDNPAGPLYLEGENAVEDAA

Core:

RAPITHSTSISTCPTGRCYCCM

Core with dehydrations:

RAPIDhbHDhaDhbDhaIDhaDhbCysPDhbGRCysYCysCysM

antiSMASH-DB 4.0 matches for BLV01_RS07955

100.0%

NZ_CP013140.1and 3 others:

NZ_CP067396.1 Lanthipeptides: JHW41_RS13260_lanthipeptideNZ_CP110813.1 Lanthipeptides: BV903_RS14555_lanthipeptideNZ_FNPT01000003.1 Lanthipeptides: BLV01_RS07955_lanthipeptide

Lanthipeptides: GLE_RS10670_lanthipeptide

1

RAPITHSTSISTCPTGRCYCCM

22

RAPITHSTSISTCPTGRCYCCM

1

RAPITHSTSISTCPTGRCYCCM

22

86.4%

NZ_AP014940.1and 1 other:

NZ_FNOG01000005.1 Lanthipeptides: BLU84_RS14520_lanthipeptide

Lanthipeptides: LEN_RS12910_lanthipeptide

1

RAPITHSTSISTCPTGRCYCCM

22

RAPITHSTS+STCP+GRC CCM

1

RAPITHSTSVSTCPSGRCICCM

22

81.8%

NZ_CP067395.1:

Lanthipeptides: JHW38_RS03600_lanthipeptide

1

RAPITHSTSISTCPTGRCYCCM

22

RAPITHSTS+STCP+GRC CC+

1

RAPITHSTSVSTCPSGRCICCI

22

with 14 more sequence matches

Only the first 10 are displayed here.

81.8%

NZ_CP072597.1:

Lanthipeptides: J5226_RS17490_lanthipeptide

1

RAPITHSTSISTCPTGRCYCCM

22

RAPITHSTS+STCP+GRC CC+

1

RAPITHSTSVSTCPSGRCVCCI

22

30.4%

NZ_CP050134.2and 3 others:

NZ_EQ973330.1 Lanthipeptides: CORMATOL_RS14310_lanthipeptideNZ_LR134504.1 Lanthipeptides: EL293_RS13085_lanthipeptideNZ_LR134504.1 Lanthipeptides: EL293_RS13085_lanthipeptide

Lanthipeptides: HBA49_RS00775_lanthipeptide

9

SISTCPTGRC

18

SI+ CPT RC

10

SIALCPTTRC

19

29.6%

NZ_LT630004.1:

Lanthipeptides: BQ5796_RS18310_lanthipeptide

4

ITHSTSISTCPTGRC

18

IT + S+S CPT +C

9

ITVTISVSMCPTTKC

23

29.2%

NZ_LIXJ01000004.1:

Lanthipeptides: allorf_208254_208481_lanthipeptide

4

ITHSTSISTCPTGRCYCCM

22

T+ +TCP G C+C +

1

FVRGTTSATCPAG-CFCSL

18

29.2%

NZ_LR134483.1and 1 other:

NZ_LR134483.1 Lanthipeptides: EL241_RS13590_lanthipeptide

Lanthipeptides: EL241_RS13590_lanthipeptide

4

ITHSTSISTCPTGRC

18

+T SI+ CPT +C

5

VTVPLSIALCPTAKC

19

27.3%

NZ_JAAXRC010000001.1:

Lanthipeptides: allorf_590704_590931_lanthipeptide

13

CPTGRCYCCM

22

CPTGR Y +

2

CPTGRRYWSV

11

26.7%

NZ_BQME02000001.1and 5 others:

NZ_BQME02000001.1 Lanthipeptides: MFF31_RS04085_lanthipeptideNZ_BQME02000001.1 Lanthipeptides: MFF31_RS04090_lanthipeptideNZ_BQME02000001.1 Lanthipeptides: MFF31_RS04090_lanthipeptideNZ_BQME02000001.1 Lanthipeptides: MFF31_RS04080_lanthipeptideNZ_BQME02000001.1 Lanthipeptides: MFF31_RS04080_lanthipeptide

Lanthipeptides: MFF31_RS04085_lanthipeptide

1

RAPITHSTSISTCPTGRC

18

R I S++ CPT RC

4

RWTIIIPISLAACPTTRC

21

24.1%

NZ_CP042598.1and 5 others:

NZ_CP042598.1 Lanthipeptides: FUT28_RS13815_lanthipeptideNZ_JADPDT010000005.1 Lanthipeptides: I4V19_RS09875_lanthipeptideNZ_JADPDT010000005.1 Lanthipeptides: I4V19_RS09875_lanthipeptideNZ_RKNR01000012.1 Lanthipeptides: EGW35_RS11625_lanthipeptideNZ_RKNR01000012.1 Lanthipeptides: EGW35_RS11625_lanthipeptide

Lanthipeptides: FUT28_RS13815_lanthipeptide

9

SISTCPTGRC

18

SIS CPT +C

15

SISLCPTTKC

24

23.3%

NZ_FOBE01000018.1:

Lanthipeptides: BMW61_RS21935_lanthipeptide

3

PITHSTSISTCPTGRC

18

PIT + S + CP+ +C

11

PITIAASTAFCPSIKC

26

23.3%

NZ_KE384107.1:

Lanthipeptides: G586_RS26715_lanthipeptide

3

PITHSTSISTCPTGRC

18

PI + S + CPT +C

11

PIIIAASTAFCPTIKC

26

MIBiG 4.0 matches for BLV01_RS07955

17.6%

Lanthipeptide: nisin A

8

TSISTCPTG

16

TSIS C G

2

TSISLCTPG

10

allorf_246742_246903

Leader:

MSGGSKPHTDGGRIPLAGVVLAA

Core:

LPTQVHASPCLDVRHEKPTNDGVGAAVWGV

Core with dehydrations:

LPDhbQVHADhaPCysLDVRHEKPDhbNDGVGAAVWGV

antiSMASH-DB 4.0 matches for allorf_246742_246903

100.0%

NZ_FNPT01000003.1:

Lanthipeptides: allorf_246742_246903_lanthipeptide

1

LPTQVHASPCLDVRHEKPTNDGVGAAVWGV

30

LPTQVHASPCLDVRHEKPTNDGVGAAVWGV

1

LPTQVHASPCLDVRHEKPTNDGVGAAVWGV

30

76.7%

Lanthipeptides: allorf_2669938_2670054_lanthipeptide

1

LPTQVHASPCLDVRHEKPTNDGVGAAVWGV

30

LPTQ +AS CL+VRHEKPT+DGVGAA+ GV

1

LPTQANASACLNVRHEKPTDDGVGAALRGV

30

30.0%

NZ_JAGPXN010000019.1:

Lanthipeptides: allorf_013482_013628_lanthipeptide

8

SPCLDVRHEKPTNDGVGAAVW

28

S C R +PT VG A W

3

SRCCRTRSSRPTACRVGCASW

23

with 10 more sequence matches

25.8%

NZ_CP063231.1:

Lanthipeptides: allorf_2149696_2149923_lanthipeptide

7

ASPCLDVRHEKPTNDGVGAAVW

28

+ P D R +P ND A W

1

SQPAGDRRQREPANDRWEPACW

22

24.2%

NZ_CP070496.1:

Lanthipeptides: JQS30_RS16095_lanthipeptide

11

LDVRHEKPTNDGVGAAVWG

29

L V PTN G+ WG

10

LTVTVCSPTNTMCGSCSWG

28

23.3%

NZ_CP063231.1and 1 other:

NZ_FPIS01000001.1 Lanthipeptides: allorf_0577798_0577908_lanthipeptide

Lanthipeptides: allorf_2141123_2141233_lanthipeptide

14

RHEKPTNDGVGAA

26

RHEKP G+G +

3

RHEKPIACGIGKS

15

20.0%

NC_014666.1and 1 other:

NC_014666.1 Lanthipeptides: FRAEUI1C_RS34465_lanthipeptide

Lanthipeptides: FRAEUI1C_RS34465_lanthipeptide

18

PTNDGVG

24

PTNDG G

2

PTNDGCG

8

16.7%

NZ_CP097290.1:

Lanthipeptides: allorf_018559_018753_lanthipeptide

1

LPTQVHASPCLDVRHE

16

LP V A+P L RHE

7

LPRGVRAAPLLTHRHE

22

16.7%

NZ_JACBYW010000001.1and 1 other:

NZ_JACBYW010000001.1 Lanthipeptides: FHR84_RS02495_lanthipeptide

Lanthipeptides: FHR84_RS02495_lanthipeptide

17

KPTNDGVG

24

+PT+DG G

4

RPTDDGCG

11

16.7%

NZ_KI912225.1and 1 other:

NZ_KI912225.1 Lanthipeptides: SACPADRAFT_RS26660_lanthipeptide

Lanthipeptides: SACPADRAFT_RS26660_lanthipeptide

17

KPTNDGVGAAV

27

+PT+DG G+

4

RPTDDGCGSTC

14

14.6%

NZ_CP079218.1:

Lanthipeptides: allorf_1926255_1926413_lanthipeptide

10

CLDVRHEKPTNDGV

23

CL+VR +P N +

20

CLEVRSREPLNITI

33

14.0%

NZ_CP094446.1:

Lanthipeptides: L3476_RS23825_lanthipeptide

14

RHEKPTNDGVGAAVWGV

30

R KP +G+A+ G+

18

RSSKPCISAIGSAISGI

34

11.3%

NZ_KB913021.1:

Lanthipeptides: NATTI_RS0124330_lanthipeptide

18

PTNDGVGAAV

27

PT+DG AA+

5

PTDDGFKAAM

14

allorf_247480_247674

Leader:

MLRAWAAKTCAPRFIPCVRNANRLAREPSGLRARLGA

Core:

LRLEQNASRTRHFGLRTQQAERCTQAE

Core with dehydrations:

LRLEQNADhaRDhbRHFGLRDhbQQAERCysDhbQAE

antiSMASH-DB 4.0 matches for allorf_247480_247674

100.0%

NZ_FNPT01000003.1:

Lanthipeptides: allorf_247480_247674_lanthipeptide

1

LRLEQNASRTRHFGLRTQQAERCTQAE

27

LRLEQNASRTRHFGLRTQQAERCTQAE

1

LRLEQNASRTRHFGLRTQQAERCTQAE

27

allorf_247520_247756

Leader:

MRGRERGFMACAQAAETRPQHFADRPQPIRLACNAQLVAFA

Core:

SQNASCARRFAPNVKHRALRASQKARAQGDWRCERKE

Core with dehydrations:

DhaQNADhaCysARRFAPNVKHRALRADhaQKARAQGDWRCysERKE

antiSMASH-DB 4.0 matches for allorf_247520_247756

100.0%

NZ_FNPT01000003.1:

Lanthipeptides: allorf_247520_247756_lanthipeptide

1

SQNASCARRFAPNVKHRALRASQKARAQGDWRCERKE

37

SQNASCARRFAPNVKHRALRASQKARAQGDWRCERKE

1

SQNASCARRFAPNVKHRALRASQKARAQGDWRCERKE

37

37.3%

NZ_CP067396.1:

Lanthipeptides: allorf_3216531_3216746_lanthipeptide

1

SQNASCARRFAPNVKHRALRASQKARAQGD

30

SQNASCARRFAP+ K ALRA QKAR Q +

30

SQNASCARRFAPSAKRHALRARQKARVQAN

59

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

Additional weak hits (2):

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.86 out of 19.52

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.55 out of 28.14

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

BLV01_RS07955 - Class II

Cleavage pHMM score: -6.40

RODEO score: 0

Monoisotopic mass: 2261.0 Da

Molecular weight: 2262.6 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 2280.7 Da N = 1; 2298.7 Da N = 2; 2316.7 Da N = 3

allorf_246742_246903 - Class II

Cleavage pHMM score: -5.60

RODEO score: 0

Monoisotopic mass: 3098.6 Da

Molecular weight: 3100.5 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3118.5 Da N = 1; 3136.5 Da N = 2

allorf_247480_247674 - Class II

Cleavage pHMM score: -3.60

RODEO score: 0

Monoisotopic mass: 3126.6 Da

Molecular weight: 3128.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3146.4 Da N = 1; 3164.5 Da N = 2; 3182.5 Da N = 3

allorf_247520_247756 - Class II

Cleavage pHMM score: -5.40

RODEO score: 0

Monoisotopic mass: 4229.2 Da

Molecular weight: 4231.7 Da

Number of bridges: 2

Additional modifications: Lac

Alternative weights: (assuming N unmodified Ser/Thr residues); 4249.7 Da N = 1

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNPT01000003.1 - Region 3 - NRPS,transAT-PKS

Download region SVG

Location: 279,257 - 368,992 nt. (total: 89,736 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

BLV01_RS08210: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BLV01_RS08215: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BLV01_RS08255: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: acylase

BLV01_RS08240: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: dehalogenase hydrolase

BLV01_RS08230: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

BLV01_RS08280: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: dehydratase

BLV01_RS08185: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthase

BLV01_RS08220: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.76	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.71	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.71	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.68	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.66	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 18.25 out of 18.46

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.90 out of 26.85

Confidence: medium

Additional weak hits (14):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.97 out of 39.62

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.50 out of 20.97

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.39 out of 30.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.05 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.92 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.03 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.74 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.39 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.17 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.92 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.37 out of 18.57

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.68 out of 21.55

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.88 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.58 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 279256 - 368992:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NMe-Thr - Tyr - Gly - ohmal - ohmal - mal) + (Phe - Me-ccmal - ccmal - mal) + (ohmal - Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS08210: Thr - Tyr - Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (32..436): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAAHFDFSVWEGNQIFGGEVNMYGITETTVHVTE
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1495..1885): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDASVSETTLVVAGEINAYGPTEATVCATT
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2546..2944): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: LSLTFDIAGLEMQALCGGEWNLYGPTETTIWSTL
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

BLV01_RS08215: Phe

Search NORINE for peptide: strict or relaxed

AMP-binding (469..863): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDASVWEMTLIVAGEVNAYGPSETTVCATF
Nearest Stachelhaus code(s):: Phe DAWTIAAVCK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

BLV01_RS08240: mal

PKS_AT (4..287): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 115.1
Methoxymalonyl-CoA: 41.1
inactive: 36.4
Methylmalonyl-CoA: 34.0
Propionyl-CoA: 31.5
fatty_acid: 20.3
Isobutyryl-CoA: 7.3
Ethylmalonyl-CoA: 6.6
Benzoyl-CoA: 4.6
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
RpoN	Regulator of nitrogen assimilation and virulence
RutR	Regulator of the pyrimidine and purine metabolism

NZ_FNPT01000010.1 - Region 1 - NRPS

Download region SVG

Location: 84,356 - 128,186 nt. (total: 43,831 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase phosphate reductase synthase

BLV01_RS15705: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

BLV01_RS15750: N-acetyl-gamma-glutamyl-phosphate reductase

smcogs: SMCOG1219: N-acetyl-gamma-glutamyl-phosphate reductase

BLV01_RS15805: riboflavin biosynthesis protein RibD

smcogs: SMCOG1240: riboflavin biosynthesis protein RibD

Transferases: acetyltransferase carbamoyltransferase kinase

BLV01_RS15755: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

BLV01_RS15760: acetylglutamate kinase

smcogs: SMCOG1174: acetylglutamate kinase

BLV01_RS15775: ornithine carbamoyltransferase

smcogs: SMCOG1114: ornithine carbamoyltransferase

Hydrolases: deacetylase dehalogenase synthase

BLV01_RS15720: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

BLV01_RS15765: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

BLV01_RS15805: riboflavin biosynthesis protein RibD

smcogs: SMCOG1240: riboflavin biosynthesis protein RibD

Lyases: lyase

BLV01_RS15740: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Ligases: ligase synthase

BLV01_RS15700: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS15715: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS15770: argininosuccinate synthase

smcogs: SMCOG1291: argininosuccinate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001758.4	0.65	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001240.4	0.63	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000357.5	0.62	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001758.4	0.65	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001240.4	0.63	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0000357.5	0.62	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.29 out of 27.55

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 21.26 out of 24.78

Confidence: medium

Additional weak hits (12):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.25 out of 32.74

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.22 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.37 out of 27.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.84 out of 21.84

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.85 out of 18.57

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.61 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.43 out of 26.06

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.03 out of 25.88

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.25 out of 26.33

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.90 out of 18.57

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 21.04 out of 30.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.04 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 84355 - 128186:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS15700: NH2

CAL_domain (149..464): NH2

minowa: NH2

Prediction, score:

NH2: 31.9
fatty_acid: 28.8
shikimic_acid: 0.0
Acetyl-CoA: 0.0
AHBA: 0.0

BLV01_RS15715: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (475..865): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDVSFDLSVYDGNSMLSGDISLGGATEASIWSIW
Nearest Stachelhaus code(s):: Cys DLYNMSLIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
GnfM	Regulator of nitrogen-fixation genes
LexA variant 2	Repressor of DNA damage response

NZ_FNPT01000001.1 - Region 1 - NRP-metallophore,NRPS

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Location: 1 - 61,966 nt. (total: 61,966 nt) Show pHMM detection rules used

Region on contig edge.

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLV01_RS00085: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acyltransferase

BLV01_RS00010: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

BLV01_RS00015: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Hydrolases: hydrolase protease

BLV01_RS00100: isochorismatase

smcogs: SMCOG1027: isochorismatase

BLV01_RS00170: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

BLV01_RS26690: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Isomerases: isomerase

BLV01_RS00110: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

BLV01_RS00005: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS00095: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLV01_RS00105: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001132.4	0.91	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.91	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.91	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.90	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.90	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002075.3	0.88	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001825.4	0.87	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.84	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.84	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	0.83	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001132.4	0.76	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.76	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.76	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000343.5	0.75	NRPS	enterobactin	Pseudomonas sp. J465
BGC0002075.3	0.75	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001825.4	0.74	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.73	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.60 out of 19.26

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.13 out of 29.72

Confidence: medium

Additional weak hits (15):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.64 out of 18.57

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.05 out of 30.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.11 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.91 out of 29.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.84 out of 36.54

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.49 out of 28.14

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.58 out of 18.57

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.75 out of 21.55

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.73 out of 19.26

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.20 out of 26.12

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.37 out of 25.88

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.27 out of 18.57

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.00 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.73 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.35 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 61966:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr) + (X - Pro - X) + (D-Asp - Thr)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS00005: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (1..357): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAAHFDFAVWEGNQVFGGEVNMYGITETTVHVSF
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLV01_RS00010: X - Pro - X

Search NORINE for peptide: strict or relaxed

AMP-binding (536..919): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YAVAFDISVEECSLAIGGELNTYGPTEATVAATW
Nearest Stachelhaus code(s):: Lys DNESGGTVAK (76% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1576..1971): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LAQTFDVSFQETFLAVAGEVNHYGPTETHVVTAH
Nearest Stachelhaus code(s):: Pro DVQFAAHVVK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2613..2998): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YAAAFDIAIEELSLVLGGEINTYGPTETTVVASH
Nearest Stachelhaus code(s):: Gln or Tyr DAESVGTVVK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLV01_RS00015: Asp - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (513..904): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YGVSADLGFTAGKIVTGGEVNHYGPTETTVGILT
Nearest Stachelhaus code(s):: Asp DLTKVGHVGK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1997..2395): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAAHFDFAVWEGNQVFGGEINMYGITETTVHVSF
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLV01_RS00105: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (37..440): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SESLFPMSSPGGALQIGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PMPAQGVVNK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
NtrC	Nitrogen regulatory protein C

NZ_FNPT01000001.1 - Region 2 - NRPS,RiPP-like

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Location: 408,485 - 467,896 nt. (total: 59,412 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

BLV01_RS01725: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Ligases: ligase synthase

BLV01_RS01690: pseudouridine synthase

smcogs: SMCOG1279: pseudouridine synthase

BLV01_RS01715: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLV01_RS01720: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001650.4	0.80	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0001249.5	0.42	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.41	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.41	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.40	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.40	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.39	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.37	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.37	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.36	NRPS	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001650.4	0.87	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.71	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 24.07 out of 30.11

Confidence: medium

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.69 out of 30.11

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 21.40 out of 23.05

Confidence: strong

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.09 out of 26.85

Confidence: medium

Additional weak hits (20):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.26 out of 30.22

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.56 out of 21.84

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 21.02 out of 29.25

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.42 out of 26.12

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.21 out of 20.01

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.50 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.35 out of 26.06

Confidence: weak

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.67 out of 29.72

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.64 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.31 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.82 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.76 out of 21.84

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.61 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.83 out of 29.38

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.50 out of 30.22

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.35 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.58 out of 26.06

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.83 out of 26.12

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.68 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 408484 - 467896:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Pro) + (Pro - X) + (X - Pro - X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS01715: X - Pro - X

Search NORINE for peptide: strict or relaxed

AMP-binding (32..443): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LTEAFDASVLEMLLMLGGEINAYGPTEITVCATQ
Nearest Stachelhaus code(s):: Leu DAWLLGAVCK (76% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1101..1517): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVAFQEGHLVVAGEHNHYGPTETHVAVAC
Nearest Stachelhaus code(s):: Pro DVQHAAHVAK (88% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (2193..2598): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDASVFDMTLLLGGEINAYGPTEITVCASA
Nearest Stachelhaus code(s):: Leu DAFILGAVCK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLV01_RS01720: Pro - X

Search NORINE for peptide: strict or relaxed

AMP-binding (30..454): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVAFQEGHLVVAGEHNHYGPTETHVVSAH
Nearest Stachelhaus code(s):: Pro DVQHIAHVVK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1115..1516): X

nrpys: Leu, Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FSQAFDASLIELYLLVGGEVHVYGPTETTVLATA
Nearest Stachelhaus code(s):: Leu or Ile DAIYLGVVLK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BLV01_RS01725: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (521..964): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVAFQEGHLAVAGEHNHYGPSETHVATAL
Nearest Stachelhaus code(s):: Pro DVQHAAHVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CelR	Cellobiose uptake repressor
RutR	Regulator of the pyrimidine and purine metabolism

NZ_FNPT01000001.1 - Region 3 - NRPS,T1PKS

Download region SVG

Location: 677,660 - 727,145 nt. (total: 49,486 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLV01_RS02885: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

BLV01_RS02890: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

BLV01_RS02895: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

BLV01_RS02900: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

BLV01_RS02925: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase

BLV01_RS02880: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Translocases: permease

BLV01_RS02920: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000999.4	1.28	NRPS, PKS	heat-stable antifungal factor	Lysobacter enzymogenes
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001276.3	0.65	PKS	6-methylsalicyclic acid	Aspergillus terreus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000999.4	0.86	NRPS, PKS	heat-stable antifungal factor	Lysobacter enzymogenes
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001244.3	0.67	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.66	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001276.3	0.66	PKS	6-methylsalicyclic acid	Aspergillus terreus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.02 out of 23.79

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 22.43 out of 24.78

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.14 out of 25.31

Confidence: medium

Additional weak hits (20):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.71 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.45 out of 26.06

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.51 out of 25.88

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 22.26 out of 31.73

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.28 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.21 out of 28.14

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.17 out of 25.88

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.99 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.86 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.61 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.22 out of 27.78

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.57 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.95 out of 32.74

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.96 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.96 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.00 out of 29.65

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.44 out of 35.63

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.07 out of 30.98

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 677659 - 727145:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk - Orn)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS02880: pk - Orn

Search NORINE for peptide: strict or relaxed

PKS_AT (582..878): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 58.3%; CE-Malonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 115.7
Methoxymalonyl-CoA: 111.7
Malonyl-CoA: 106.9
Ethylmalonyl-CoA: 97.2
Isobutyryl-CoA: 93.7
Propionyl-CoA: 66.2
2-Methylbutyryl-CoA: 64.7
trans-1,2-CPDA: 55.5
Acetyl-CoA: 55.1
Benzoyl-CoA: 53.3
3-Methylbutyryl-CoA: 36.8
inactive: 36.7
fatty_acid: 36.6
CHC-CoA: 32.8

AMP-binding (2334..2745): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: AGWAFDVFAGDREFVVGSDINSYGLSEATIDSTY
Nearest Stachelhaus code(s):: Orn DVGEVGSIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
IolR	Regulator of the inositol catabolism
LexA variant 2	Repressor of DNA damage response

NZ_FNPT01000001.1 - Region 4 - RiPP-like

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Location: 816,064 - 826,954 nt. (total: 10,891 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001778.5	0.30	other (other)	showdomycin	Streptomyces showdoensis
BGC0002046.3	0.27	NRPS, PKS	lobatamide A	Gynuella sunshinyii YC6258
BGC0000188.5	0.27	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0002012.4	0.22	PKS	julichrome Q3-3, julichrome Q3-5	Streptomyces afghaniensis 772
BGC0000344.5	0.20	NRPS	entolysin	Pseudomonas entomophila L48
BGC0000651.5	0.20	terpene	KS-505a	Streptomyces argenteolus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001778.5	0.30	other (other)	showdomycin	Streptomyces showdoensis
BGC0002046.3	0.27	NRPS, PKS	lobatamide A	Gynuella sunshinyii YC6258
BGC0000188.5	0.27	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0002012.4	0.22	PKS	julichrome Q3-3, julichrome Q3-5	Streptomyces afghaniensis 772
BGC0000344.5	0.20	NRPS	entolysin	Pseudomonas entomophila L48
BGC0000651.5	0.20	terpene	KS-505a	Streptomyces argenteolus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.64 out of 26.85

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.98 out of 20.97

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.42 out of 20.47

Confidence: medium

Additional weak hits (4):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.79 out of 30.22

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.18 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.72 out of 30.98

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.03 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
RutR	Regulator of the pyrimidine and purine metabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNPT01000001.1 - Region 5 - terpene-precursor

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Location: 874,156 - 895,097 nt. (total: 20,942 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

BLV01_RS03585: NADH:flavin oxidoreductase/NADH oxidase

smcogs: SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase

Transferases: transferase

BLV01_RS03575: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Ligases: synthetase

BLV01_RS03600: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002219.2	0.39	terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000639.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000641.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000642.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000638.3	0.37	terpene	carotenoid	Pantoea ananatis
BGC0000640.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000635.3	0.37	terpene	carotenoid	Paracoccus sp. N81106

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002219.2	0.39	terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000639.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000641.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000642.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000638.3	0.37	terpene	carotenoid	Pantoea ananatis
BGC0000640.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000635.3	0.37	terpene	carotenoid	Paracoccus sp. N81106

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

BLV01_RS03600: PT_FPPS_like

Domain 1 (51 - 312): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 21.26 out of 32.93

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.47 out of 20.97

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.78 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.18 out of 27.78

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description

NZ_FNPT01000001.1 - Region 6 - NRPS,transAT-PKS

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Location: 1,173,581 - 1,321,872 nt. (total: 148,292 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

BLV01_RS04880: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

Transferases: PPTase acylase acyltransferase methyltransferase

BLV01_RS04890: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

BLV01_RS05000: methyltransferase

smcogs: SMCOG1089: methyltransferase

BLV01_RS05010: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLV01_RS05015: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Ligases: ligase synthase

BLV01_RS04885: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS04960: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS04965: carbamoyl phosphate synthase small subunit

smcogs: SMCOG1249: carbamoyl phosphate synthase small subunit

BLV01_RS04995: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLV01_RS05005: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	1.03	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.99	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.98	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000307.4	0.95	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.95	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.94	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.94	NRPS	HC-toxin	Alternaria jesenskae
BGC0001132.4	0.94	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.93	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001641.4	0.90	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000461.5	0.75	NRPS	WAP-8294A2	Lysobacter enzymogenes
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.73	NRPS	enniatin	Fusarium equiseti
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.71	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 20.47 out of 20.47

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 18.15 out of 18.46

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.79 out of 20.97

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.68 out of 19.26

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.41 out of 27.78

Confidence: medium

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 18.58 out of 21.84

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.91 out of 20.97

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.64 out of 27.78

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.69 out of 26.85

Confidence: medium

Additional weak hits (46):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.79 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.63 out of 27.78

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.34 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.33 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.68 out of 26.06

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.20 out of 39.62

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.52 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.83 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.03 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.84 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.09 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.76 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.79 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.93 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.98 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.57 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 19.96 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.32 out of 29.25

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.76 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.38 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.27 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.02 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.13 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.83 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.36 out of 26.06

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.51 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.16 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.15 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.27 out of 28.19

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.14 out of 24.78

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.32 out of 30.98

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.07 out of 30.98

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.75 out of 24.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.39 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.95 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 20.90 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.12 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.94 out of 28.14

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.08 out of 29.25

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 18.16 out of 28.14

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.78 out of 26.12

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.35 out of 18.57

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.54 out of 25.88

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.45 out of 18.57

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.28 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 7, location 1173580 - 1321872:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - Gly - D-NMe-Phe - Leu - D-Orn) + (Glu - D-Asn - D-Trp - D-Orn - NMe-Val) + (Gly - Me-ccmal) + (ccmal - ohmal - Me-ccmal - mal - ohmal - Me-ohmal) + (Me-mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 8, location 1173580 - 1255839:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - Gly - D-NMe-Phe - Leu - D-Orn) + (Glu - D-Asn - D-Trp - D-Orn - NMe-Val)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 9, location 1241751 - 1321872:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly - Me-ccmal) + (ccmal - ohmal - Me-ccmal - mal - ohmal - Me-ohmal) + (Me-mal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS04885: Ser - Gly - Phe - Leu - Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (460..863): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAIDVTA
Nearest Stachelhaus code(s):: Ser DVWHVSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1517..1918): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDIAGLELQALCGGEWNVYGPTETTVWSLL
Nearest Stachelhaus code(s):: Gly DILQLGVVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2584..2977): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDAAFWEMTLIVAGETNAYGPTETTVCATM
Nearest Stachelhaus code(s):: Phe DAWTIAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4530..4936): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSQAFDAAAMEMLIIVGGEVNAYGPTEITICAAM
Nearest Stachelhaus code(s):: Leu DAMLIGAICK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5593..6017): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LHHAFDTSLEGFDLMLGGEHNYYGPTEYTVDAGG
Nearest Stachelhaus code(s):: Orn DTEDMGYVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BLV01_RS04890: Glu - Asn - Trp - Orn - Val

Search NORINE for peptide: strict or relaxed

AMP-binding (462..864): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCGGEHHSYGPTETSIGVAA
Nearest Stachelhaus code(s):: Glu DVWHVGSIGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1528..1939): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWCLFDGSVTLGKLLLGGEINEYGPTEATVGCST
Nearest Stachelhaus code(s):: Asn DGTKLGEVGK (94% 8Å match); OH-Asn or Asn DGTKLGEVGK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3063..3477): Trp

nrpys: Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SARPFDASLGTHLSVLGGEYNEYGPTEASVWSSA
Nearest Stachelhaus code(s):: Trp DAGLVGEVWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4602..5010): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: LHHAFDTSIEAWDVMLGGEYNLYGPTECTVDACF
Nearest Stachelhaus code(s):: Orn DTEDMGLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6129..6531): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: FDRSFDASTYEVWLWAGGDVNGYGPTETTTFALT
Nearest Stachelhaus code(s):: Val DAYWWGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BLV01_RS04960: X

AMP-binding (10..396): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: TNYSFAYGLNSMFAVSAGEVDGIGTTEVLSTFIS
Nearest Stachelhaus code(s):: pNH2-Bz AYNFIAGSTK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLV01_RS04995: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (471..869): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDISVLELQALCGGEWNLYGPTETTVWSTI
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

BLV01_RS05010: mal

PKS_AT (4..278): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%; Ethylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 113.8
inactive: 46.1
Methoxymalonyl-CoA: 41.5
Methylmalonyl-CoA: 34.5
Propionyl-CoA: 18.5
Isobutyryl-CoA: 16.5
CHC-CoA: 14.2
2-Methylbutyryl-CoA: 12.3
Acetyl-CoA: 11.8
Benzoyl-CoA: 11.2
Ethylmalonyl-CoA: 8.0
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

BLV01_RS05015: mal

PKS_AT (258..535): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 42.4
Methoxymalonyl-CoA: 25.3
inactive: 21.1
Methylmalonyl-CoA: 10.7
Propionyl-CoA: 7.9
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MexT	Global virulence regulator
RpoN	Regulator of nitrogen assimilation and virulence
RutR	Regulator of the pyrimidine and purine metabolism
ToxT	Regulator of MSHA biosynthesis
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNPT01000012.1 - Region 1 - lanthipeptide-class-ii

Download region SVG

Location: 25,954 - 48,773 nt. (total: 22,820 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

BLV01_RS16845: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: ligase

BLV01_RS16805: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.56	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001825.4	0.55	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001579.4	0.49	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002704.2	0.46	ribosomal	archalan β	Halorussus salinus
BGC0002698.2	0.46	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000503.5	0.43	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002171.2	0.42	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.42	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0001873.3	0.42	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0000900.5	0.42	other (other)	ferrichrome	Aspergillus oryzae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.56	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001825.4	0.55	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001579.4	0.49	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0002704.2	0.46	ribosomal	archalan β	Halorussus salinus
BGC0002698.2	0.46	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000503.5	0.43	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0002171.2	0.42	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger ATCC 1015
BGC0002275.2	0.42	NRPS, PKS	pyrophen, campyrone B	Aspergillus niger CBS 101883
BGC0001873.3	0.42	NRPS	pyrrolizixenamide A	Xenorhabdus szentirmaii DSM 16338
BGC0000900.5	0.42	other (other)	ferrichrome	Aspergillus oryzae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BLV01_RS16845

allorf_039170_039331

Leader:

MSIAASVVRMRTCGPVRENAASASS

Core:

RRSKAFVHIWCGATMKLTVPRRTFGDEL

Core with dehydrations:

RRDhaKAFVHIWCysGADhbMKLDhbVPRRDhbFGDEL

antiSMASH-DB 4.0 matches for allorf_039170_039331

100.0%

Lanthipeptides: allorf_039170_039331_lanthipeptide

1

RRSKAFVHIWCGATMKLTVPRRTFGDEL

28

RRSKAFVHIWCGATMKLTVPRRTFGDEL

1

RRSKAFVHIWCGATMKLTVPRRTFGDEL

28

28.6%

NZ_CP084541.1:

Lanthipeptides: LC193_RS28955_lanthipeptide

11

CGATMKLTVPRRTF

24

CG LT P RTF

3

CGLGTLLTCPTRTF

16

27.0%

NZ_CP096281.1:

Lanthipeptides: allorf_2545680_2545820_lanthipeptide

1

RRSKAFVHIWCGAT-----MKLTVPR

21

RRS F + CG T L +PR

7

RRSDTFSQVGCGGTGLDRRASLRLPR

32

with 4 more sequence matches

21.9%

NZ_JAOPQW010000026.1:

Lanthipeptides: allorf_25777_25899_lanthipeptide

4

KAFVHIWCGATMKLTVPRRT

23

K F++IW G P++T

1

KIFIYIWMGMEEVTLDPKQT

20

21.6%

NZ_CP061072.1:

Lanthipeptides: allorf_5574385_5574531_lanthipeptide

1

RRSKAFVHIWCGAT

14

RRS AF + CG+T

7

RRSGAFAQVGCGST

20

19.4%

NZ_SBIT01000010.1:

Lanthipeptides: allorf_176054_176206_lanthipeptide

21

RRTFGDEL

28

RR+F DEL

5

RRSFSDEL

12

18.9%

NZ_CP080029.1:

Lanthipeptides: allorf_2623795_2623935_lanthipeptide

1

RRSKAFVHIWCGAT

14

RRS F + CG T

7

RRSDTFPQVGCGGT

20

allorf_039443_039604

Leader:

MPTRSPAPESKAPMAAA

Core:

QSLFRGARSQRDHDLWQASRRAIGAHSPGVGRCAEP

Core with dehydrations:

QDhaLFRGARDhaQRDHDLWQADhaRRAIGAHDhaPGVGRCysAEP

antiSMASH-DB 4.0 matches for allorf_039443_039604

100.0%

Lanthipeptides: allorf_039443_039604_lanthipeptide

1

QSLFRGARSQRDHDLWQASRRAIGAHSPGVGRCAEP

36

QSLFRGARSQRDHDLWQASRRAIGAHSPGVGRCAEP

1

QSLFRGARSQRDHDLWQASRRAIGAHSPGVGRCAEP

36

36.1%

NZ_CP040916.1:

Lanthipeptides: allorf_9190079_9190246_lanthipeptide

5

RGARSQRDHDLWQASRRAIGAHS-PGVG

31

RG R +RD + +R+A GA + PGVG

1

RGRRGRRDAFAHRWARKACGAVTFPGVG

28

17.6%

NC_021252.1:

Lanthipeptides: allorf_4521129_4521314_lanthipeptide

11

RDHDLWQASRRAIGAHSP

28

R+ D+ ++ RAIGAH P

27

REPDITTSTGRAIGAHCP

44

with 2 more sequence matches

12.7%

NZ_NNBO01000009.1:

Lanthipeptides: allorf_135626_135823_lanthipeptide

25

AHSPGVGRCAE

35

H P +GRCAE

40

THVPLLGRCAE

50

12.3%

NZ_BMUU01000006.1and 2 others:

NZ_BMUZ01000004.1 Lanthipeptides: IE267_RS12230_lanthipeptideNZ_CP070373.1 Lanthipeptides: GWQ54_RS15580_lanthipeptide

Lanthipeptides: IE233_RS19525_lanthipeptide

15

LWQASRRAIGAHSPGV

30

+ A + + AHSPGV

4

VADAGAQGVAAHSPGV

19

allorf_041529_041675

Leader:

MGAATAAESIGS

Core:

EGPATALLCRICPHPAFARADRAALRTGPETGVKHR

Core with dehydrations:

EGPADhbALLCysRICysPHPAFARADRAALRDhbGPEDhbGVKHR

antiSMASH-DB 4.0 matches for allorf_041529_041675

100.0%

Lanthipeptides: allorf_041529_041675_lanthipeptide

1

EGPATALLCRICPHPAFARADRAALRTGPETGVKHR

36

EGPATALLCRICPHPAFARADRAALRTGPETGVKHR

1

EGPATALLCRICPHPAFARADRAALRTGPETGVKHR

36

27.5%

NZ_CP047146.1:

Lanthipeptides: allorf_3666144_3666341_lanthipeptide

15

PAFARADRAALRTGPET--GVKHR

36

PA + R RT PET V+HR

9

PAHPQTHRRRTRTSPETWCAVRHR

32

22.2%

NZ_CP054919.1:

Lanthipeptides: allorf_4709651_4709812_lanthipeptide

12

CPHPAFARADRAALRTGPETG

32

C HP A R LRT P G

24

CQHPGLAPECRDTLRTAPAPG

44

with 3 more sequence matches

16.7%

NZ_CP070499.1:

Lanthipeptides: allorf_3655244_3655348_lanthipeptide

17

FARADRAALRTGP

29

+A+ +AA+R GP

1

YAQGHQAAIRKGP

13

15.9%

NZ_JAGPNM010000006.1:

Lanthipeptides: J3B89_RS14930_lanthipeptide

12

CPHPAFARADRAALRTGPETGV

33

CP P+ +R R TG+

15

CPQPSVDSPNRPGCRISDPTGM

36

11.3%

NZ_VITO01000001.1and 1 other:

NZ_VITV01000003.1 Lanthipeptides: FBZ87_RS11200_lanthipeptide

Lanthipeptides: FBZ88_RS02505_lanthipeptide

2

GPATALLCRICPH

14

P T L C+IC H

6

SPGTKLECKICWH

18

allorf_047106_047189

Leader:

MTVGEDAASPPA

Core:

WFPRGPEPDSVEARC

Core with dehydrations:

WFPRGPEPDDhaVEARCys

antiSMASH-DB 4.0 matches for allorf_047106_047189

100.0%

Lanthipeptides: allorf_047106_047189_lanthipeptide

1

WFPRGPEPDSVEARC

15

WFPRGPEPDSVEARC

1

WFPRGPEPDSVEARC

15

80.0%

Lanthipeptides: allorf_0963999_0964082_lanthipeptide

2

FPRGPEPDSVEARC

15

FPRG PDSVEARC

2

FPRGRGPDSVEARC

15

66.7%

Lanthipeptides: allorf_4963134_4963217_lanthipeptide

2

FPRGPEPDSVEARC

15

FPR P SVEARC

2

FPRARGPGSVEARC

15

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.77 out of 29.72

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.87 out of 29.72

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.92 out of 20.47

Confidence: medium

Additional weak hits (6):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.76 out of 21.84

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.33 out of 30.97

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.90 out of 30.22

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.76 out of 30.25

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.97 out of 30.98

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.95 out of 28.19

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

allorf_039170_039331 - Class II

Cleavage pHMM score: -7.10

RODEO score: 0

Monoisotopic mass: 3202.7 Da

Molecular weight: 3204.8 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3222.8 Da N = 1; 3240.8 Da N = 2; 3258.8 Da N = 3

allorf_039443_039604 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 3900.0 Da

Molecular weight: 3902.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3920.3 Da N = 1; 3938.3 Da N = 2; 3956.3 Da N = 3

allorf_041529_041675 - Class II

Cleavage pHMM score: -3.70

RODEO score: 0

Monoisotopic mass: 3784.0 Da

Molecular weight: 3786.3 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3804.4 Da N = 1

allorf_047106_047189 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 1726.8 Da

Molecular weight: 1727.9 Da

Number of bridges: 1

TFBS definitions

Regulator	Description
NtrC	Nitrogen regulatory protein C
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNPT01000043.1 - Region 1 - NRPS

Download region SVG

Location: 1 - 6,786 nt. (total: 6,786 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

BLV01_RS25030: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.62	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.62	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.62	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.62	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 6786:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS25030: X

AMP-binding (693..1097): X

nrpys: OH-Asn, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWSLFDGSVTVGKLLLGGELNEYGPTEATVGCCV
Nearest Stachelhaus code(s):: OH-Asn or Asn DGTKLGEVGK (100% 8Å match); Asn DGTKLGEVGK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

NZ_FNPT01000020.1 - Region 1 - NRPS

Download region SVG

Location: 52,827 - 95,551 nt. (total: 42,725 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

BLV01_RS21080: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Ligases: ligase

BLV01_RS21065: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS21075: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLV01_RS21085: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.76	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.74	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002071.2	0.71	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001133.4	0.70	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.76	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.74	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002071.2	0.71	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001133.4	0.70	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.18 out of 20.47

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.22 out of 18.46

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.44 out of 23.79

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.74 out of 20.47

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.02 out of 23.79

Confidence: medium

Additional weak hits (17):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.27 out of 30.22

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.72 out of 27.04

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.91 out of 39.62

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.76 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.87 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.93 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.19 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.27 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.54 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.13 out of 26.06

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.96 out of 36.54

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.82 out of 30.98

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.17 out of 18.57

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.99 out of 29.25

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 19.71 out of 33.14

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.58 out of 30.11

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 52826 - 95551:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala) + (Val) + (Hpg - Gly - Ser)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLV01_RS21065: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (463..850): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: LWATFDLSVFEVNTNLAGEFDLYGPSEDTTYSTC
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLV01_RS21075: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (527..929): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LRMSFDVSVYESFILIGVEINGYGPTETTVCCTL
Nearest Stachelhaus code(s):: Val DMGFLGGVCK (59% 8Å match); Trp or Pro DVYFVGGICK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

BLV01_RS21080: Hpg - Gly - Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (540..931): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QSYAFDASVLDGHSVLGGEFGLYGASEASLVALA
Nearest Stachelhaus code(s):: Hpg DALHLGLLCK (47% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2412..2822): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FAMTFDIAALELQALCGGEWNLYGPTETTVWSSC
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3484..3894): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAIDVTA
Nearest Stachelhaus code(s):: Ser DVWHVSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNPT01000029.1 - Region 1 - arylpolyene

Download region SVG

Location: 23,591 - 56,895 nt. (total: 33,305 nt) Show pHMM detection rules used

Region on contig edge.

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLV01_RS23645: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: hydrolase

BLV01_RS23695: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: synthase

BLV01_RS23655: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

BLV01_RS23665: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001284.4	0.57	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000840.5	0.54	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0000867.3	0.48	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0001928.2	0.43	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002138.2	0.43	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0001197.5	0.42	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001605.3	0.40	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001606.3	0.40	PKS	gibepyrone-A	Fusarium fujikuroi IMI 58289
BGC0002510.2	0.39	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0001940.3	0.39	PKS	pyxidicycline A, pyxidicycline B	Pyxidicoccus fallax

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001284.4	0.57	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000840.5	0.54	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0000867.3	0.48	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0001928.2	0.43	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002138.2	0.43	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0001197.5	0.42	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001605.3	0.40	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0001606.3	0.40	PKS	gibepyrone-A	Fusarium fujikuroi IMI 58289
BGC0002510.2	0.39	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0001940.3	0.39	PKS	pyxidicycline A, pyxidicycline B	Pyxidicoccus fallax

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.31 out of 18.46

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.58 out of 20.97

Confidence: medium

Additional weak hits (10):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.15 out of 30.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.84 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.10 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.82 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.12 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.75 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.65 out of 29.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.76 out of 21.84

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.43 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.73 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FNPT01000016.1 - Region 1 - NRPS

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Location: 97,713 - 121,526 nt. (total: 23,814 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

BLV01_RS19500: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Ligases: ligase

BLV01_RS19555: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.59	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.59	NRPS	AbT1	Aureobasidium pullulans
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.58	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.58	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.58	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.58	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.58	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002164.2	0.58	NRPS	peramine	Epichloe festucae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.59	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000307.4	0.59	NRPS	AbT1	Aureobasidium pullulans
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.58	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.58	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.58	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.58	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.58	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002164.2	0.58	NRPS	peramine	Epichloe festucae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.67 out of 19.26

Confidence: medium

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 23.11 out of 27.18

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

Additional weak hits (7):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.11 out of 26.33

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.64 out of 21.55

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.21 out of 30.22

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.08 out of 33.14

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.05 out of 27.18

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.93 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.75 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

BLV01_RS19555: X

AMP-binding (932..1271): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FWLAFDASIGEGAFLTAGSVNAYGPTETTVLSSA
Nearest Stachelhaus code(s):: R-bmeTrp DAGALGVVLK (47% 8Å match)
Stachelhaus code match:: 80% (moderate)