LOCUS       NZ_FNPT01000001        10891 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Lysobacter sp. yr284, whole genome shotgun sequence.
ACCESSION   NZ_FNPT01000001
VERSION     NZ_FNPT01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lysobacter sp. yr284
  ORGANISM  Lysobacter sp. yr284
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Lysobacter.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900107375.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 20:31:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,139
            CDSs (total)                      :: 5,059
            Genes (coding)                    :: 5,007
            CDSs (with protein)               :: 5,007
            Genes (RNA)                       :: 80
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 52
            CDSs (without protein)            :: 52
            Pseudo Genes (ambiguous residues) :: 0 of 52
            Pseudo Genes (frameshifted)       :: 4 of 52
            Pseudo Genes (incomplete)         :: 50 of 52
            Pseudo Genes (internal stop)      :: 1 of 52
            Pseudo Genes (multiple problems)  :: 3 of 52
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:51:04
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 816063
            Orig. end                         :: 826954
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNPT01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10891
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10891
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10891
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5891](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5891
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    441..455
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.18"
                     /tool="antismash"
     misc_feature    complement(833..849)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.72"
                     /tool="antismash"
     gene            862..1767
                     /gene="pstA"
                     /locus_tag="BLV01_RS03310"
                     /old_locus_tag="SAMN04487939_101669"
     CDS             862..1767
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412770.1"
                     /locus_tag="BLV01_RS03310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101669"
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="WP_091793998.1"
                     /transl_table=11
                     /translation="MSAVANKHDPAEAARHRTADSLYRRRRVVNALSVLLACGAALFGL
                     FFLGWILYTLISKGIGGINLALFTQNTPPPMQEGGLLNAFFGSAVMCLIAIVIGTPLGI
                     AAGTWLAEYGAGRKMGTVVRFVNDILLSAPSIVLGLFVYTLVVMQTGGNFSALAGAIAL
                     AFIVLPVVVRTTDEMLRLVPPQMREAALSLGVPQWKVIMQVLYRSASAGIVTGVLLALA
                     RISGETAPLLFTAFGNQYWSHNVLQPMASVPVVMNQFAGSPYETWQTLAWAGALVLTFF
                     VLVVSLLARALVLRNRISND"
     gene            1802..2611
                     /gene="pstB"
                     /locus_tag="BLV01_RS03315"
                     /old_locus_tag="SAMN04487939_101670"
     CDS             1802..2611
                     /EC_number="7.3.2.1"
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstB"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343978.1"
                     /locus_tag="BLV01_RS03315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101670"
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="WP_091793999.1"
                     /transl_table=11
                     /translation="MNDARLSVATPSREAVGANPVKLAARDLNFYYDKFHALKSINLEI
                     PEKQVTALIGPSGCGKSTLLRIFNRIYALYPKLEARGQVLLDGDNILDPKYPMNRLRSK
                     VGMVFQKPVPFPMTIFENVAYGIRHHEKLSKAEMNDRVEHALRQGALWDEVKDKLGQSA
                     LGLSGGQQQRLCIARAVALKPSVLLLDEPTSALDPISTSRIEQLVEELKKDYTIAIVTH
                     NMQQAARVSDFTAFMYLGDLIEHGPTEQIFSKPTKQQTEDYITGRFG"
     gene            2745..3455
                     /gene="phoU"
                     /locus_tag="BLV01_RS03320"
                     /old_locus_tag="SAMN04487939_101671"
     CDS             2745..3455
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="phoU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994733.1"
                     /locus_tag="BLV01_RS03320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101671"
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="WP_091794001.1"
                     /transl_table=11
                     /translation="MSNQMHDHIVKSYDDEQRRLLDETLRMGEIAAAQLESALDVVQRR
                     DDKAAERIIANDEAIDALEQEISHDVMKLALRGPMARDLREILAAIRIASDIERVGDYA
                     ANVAKRSTALNLSPPLPHVAGLHALGTLAVKQLRDVLVAYRDNDVELAQRVRDRDAEVD
                     TAYTGLFRELLTYMMEDARSITACTHLLFMAKNIERVGDHATNIAENVWFLVKGDEPLP
                     PRDKRDVTSTTGAV"
     misc_feature    complement(2761..2776)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.64"
                     /tool="antismash"
     misc_feature    complement(3658..3669)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 16.98"
                     /tool="antismash"
     gene            3819..4334
                     /locus_tag="BLV01_RS03325"
                     /old_locus_tag="SAMN04487939_101672"
     CDS             3819..4334
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /locus_tag="BLV01_RS03325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101672"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_091794003.1"
                     /transl_table=11
                     /translation="MTSLLANGDLAAARAGDTAALERVLRHSRQDLRRYAEFHCVINDV
                     EDAVQESLIAVSRKLRDLRVLECFVSWTFRIVKRECNRLKRMRRMFSGQAITEEIAPVV
                     TPEPAEWRHDVAAALESLPAHYRRIILLRDLEGLTVAEIGAELGLSREAVKSRLHRARV
                     LAREYLSP"
     misc_feature    3828..3830
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4553..4993
                     /locus_tag="BLV01_RS03330"
                     /old_locus_tag="SAMN04487939_101673"
     CDS             4553..4993
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659080.1"
                     /locus_tag="BLV01_RS03330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101673"
                     /product="hypothetical protein"
                     /protein_id="WP_091794005.1"
                     /transl_table=11
                     /translation="MSKKINKPVALALGATLLGGLSLSASAFAMSDLSQGYMLGAQQAQ
                     TAEKAADAKATDAKKAEGSCGAKKAEGSCGADKKKAEGSCGADKKKAEGSCGGDKKKAE
                     GSCGGDKKKAEGKCGGDKSKTDAKAKKAGEGKCGEGKCGGSI"
     gene            5001..5891
                     /locus_tag="BLV01_RS03335"
                     /old_locus_tag="SAMN04487939_101674"
     CDS             5001..5891
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014036545.1"
                     /locus_tag="BLV01_RS03335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101674"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_217633433.1"
                     /sec_met_domain="DUF692 (E-value: 4.7e-111, bitscore:
                     366.2, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTMDGTHPLTPDAVGLGLRRALLGPLQSAPHAPGAARDFDFLELA
                     PENWIGVGGRYGEQLRELSSHYPLVCHGLSLSLGGTAALDETFLARVRRFLDEHGVACY
                     SEHLSYCSDDGHLYDLLPIPFTEEAVRHVAARIRRTQDIVGRRIAVENASYYAAPHQEM
                     SEIEFTRAVLAEADCDLLLDVNNVYVNSINHRYDARAFIAAMPHERIAYIHVAGHYDEA
                     DDLKVDTHGAPVKHEVWDLLGDAYRLFGPRPTLLERDFNFPPYAELVDELGTVRRILRE
                     SAAPAAREPARRARA"
     misc_feature    5033..5047
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.42"
                     /tool="antismash"
     gene            5896..6663
                     /locus_tag="BLV01_RS03340"
                     /old_locus_tag="SAMN04487939_101675"
     CDS             5896..6663
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412767.1"
                     /locus_tag="BLV01_RS03340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101675"
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="WP_091794007.1"
                     /transl_table=11
                     /translation="MNDAPSRLRAQQFELTRHLRDPAASPAPAGIEDRRLAVYRELLFN
                     NIASLLSGNFPAIERILGEQRWEKLVRDFYRDHRCHTPLFAEIAREFQRYLQDLDEREP
                     DGLDPPFLQELAHYEWVELALQLSEAQAPHDDAGHDPADLLDRAPRLSPLAWPLAYAWP
                     VHRLGPDYQPAQPPPAPTLLLLRREADGEVRFSELSPLAFRLLQRLEESPQLSAREQLR
                     ALAAEAGSDDEAGFLDMGAALLRQMRANGVVYF"
     gene            6828..7484
                     /gene="rnt"
                     /locus_tag="BLV01_RS03345"
                     /old_locus_tag="SAMN04487939_101676"
     CDS             6828..7484
                     /GO_function="GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /GO_process="GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rnt"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399265.1"
                     /locus_tag="BLV01_RS03345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101676"
                     /product="ribonuclease T"
                     /protein_id="WP_091794009.1"
                     /transl_table=11
                     /translation="MIDPGPASSAAPAAPVPATLATRFRGYLPVVVDVETGGFDWNKHA
                     LLEIAVQPIELDADGRLVPGEVASAHVVPAAGTLIDPKSLEVTGIDIDHPFRDAKPERQ
                     ALEAVFAPVREAVKRHGCQRAILVGHNAHFDLNFLNAAVARSGHKRNPFHPFSVFDTVT
                     LAGVAYGQTVLARAVQAAGFVWNGEEAHSAVYDTERTAELFCKIVNAWPARGAGG"
     gene            7504..8484
                     /locus_tag="BLV01_RS03350"
                     /old_locus_tag="SAMN04487939_101677"
     CDS             7504..8484
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLV01_RS03350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04487939_101677"
                     /product="hypothetical protein"
                     /protein_id="WP_091794010.1"
                     /transl_table=11
                     /translation="MDEPFAPPYLWIPGLGADPDALRRLRERAPRPREPMGEAWFMGGD
                     RHYYRGLMAGAAADWELGELENALTELTSGPTSFDHLDEWSLWFDYLLPRAQERADETR
                     HLHELLVSAVCVHCLDPALPGRPAEFRRDLLDTLGRALMAPQRWRDGRIVADRVLDPLR
                     SYVCGWTLESGAAFSAICSLALRYLETDAVDAWIGSLLAIDDPLWRAVFVVWLDGAEPL
                     LFEGQQPEQAEHDPMKNIAWDWCWVLRGAVPSPQLDPEARVHAFFPPGNVAALAAALRR
                     RLGLDALSQWSAALRVACGETRDPGTALWQFETSALRVAERFGLD"
     gene            8635..9246
                     /locus_tag="BLV01_RS03355"
                     /old_locus_tag="SAMN04487939_101678"
     CDS             8635..9246
                     /GO_function="GO:0004568 - chitinase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006032 - chitin catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012409.4"
                     /locus_tag="BLV01_RS03355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101678"
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="WP_175485752.1"
                     /transl_table=11
                     /translation="MFAFDDEQFLDGYRKRFGPLRDDLAQAVRFLIGRIERDASFSANP
                     VHRYQIAYCLATFKWETAHTLRPIDEFGDDARFERLYGPDTRIGKALGNTRRGDGAKYH
                     GRGYVQLTGRANYERAGKFLGIDLVGKPEDAKQPEIAYAIAMQGMKQGWFTGKKLDHYF
                     KPGQLPNYEEARRIINGQDKAQTIADIARRFDELLANALE"
     misc_feature    complement(8636..8651)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.79"
                     /tool="antismash"
     gene            complement(9383..9841)
                     /locus_tag="BLV01_RS03360"
                     /old_locus_tag="SAMN04487939_101679"
     CDS             complement(9383..9841)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660277.1"
                     /locus_tag="BLV01_RS03360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101679"
                     /product="acetyltransferase"
                     /protein_id="WP_254782488.1"
                     /transl_table=11
                     /translation="MTRAMPIRERRRDDNPRLLALWERSVRATHDFLAEADVDALRPQV
                     RDVYLDAVEIWVFEDADGLAGFVGLADAQVEMLFLEPARRGQGIGTRLLDHARGLRGAL
                     TVDVNEHNPQAHGFYRRYGFVDIGRSPLDGQGRPFPLIHMALPRDADA"
     gene            complement(9982..10293)
                     /gene="sugE"
                     /locus_tag="BLV01_RS03365"
                     /old_locus_tag="SAMN04487939_101680"
     CDS             complement(9982..10293)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="sugE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451011.1"
                     /locus_tag="BLV01_RS03365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487939_101680"
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="WP_057948212.1"
                     /transl_table=11
                     /translation="MPWVLLVFAGLFEIGWAIGLKYTEGFTRLWPSVGTVASMAISVGL
                     LGLAMKSLPVGTAYAIWVGIGAVGTVILGMVLFNEPATALRIASIVLIVAGLVGLKLA"
     misc_feature    complement(10256..10271)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.03"
                     /tool="antismash"
ORIGIN
        1 cctcatcgcc ctcgccagcg ccgcgctgtc gatgctgtgg ggcggccgcc acgtgctgga
       61 agaagaaggc atcgacttct tcatcaccgc gcagtggaac ccggtcgaga accactacgg
      121 cgcgctggtt cccatctacg gcaccatcgt caccgcgctg atcgccatgg tcatcgccgt
      181 gccggtcagc ttcggcatcg ccttcttcct gaccgaggtc gcgccgcgct gggcgcgcgg
      241 tccgatcggc accgcgatcg agctgctcgc gggcattccc tcgatcatct acggcatgtg
      301 gggcctgttc gtgctggtgc cggtgatgac cgaatacgtc accccctggc tcaacgacca
      361 cgtcggcacc tggccgctga tcggcccgct gttccgcggc ccgccgctgg gcatcggcat
      421 gctcaccgcc ggcatcgtgc tggcgatcat ggtcattccc ttcatctcct cggtgatgcg
      481 cgaagtgttc ctgaccgtgc ccacgcgcct gaaggaatcg gcctacgcgc tgggctcgac
      541 caagtgggaa gtcagctggg acatcgtctt gccctacacc cgctcggcgg tcatcggcgg
      601 catcttcctc ggcctgggcc gcgcgctcgg cgagaccatg gcggtggcct tcgtgatcgg
      661 caactcggtc aacttctccg cctcgctgct ggagccgggc accaccatcg ccgcgctgat
      721 cgccaacgac ttcggcgaag ccaccgagac ctaccgctcg gccctgctgc tgctcggctt
      781 cgtgctgttc atcgtgacct tcgtggtgtt ggccgcggcg cggctgatgc tgatgcaact
      841 cgcgcgcaag gagggcaact gatgagcgcc gtcgccaaca agcacgaccc ggccgaagcc
      901 gcgcgccacc gcaccgccga ctcgctgtac cgccgccgcc gcgtggtcaa cgcgctgtcg
      961 gtgctgctgg cctgcggcgc cgcgctgttc ggcctgttct tcctcggctg gatcctctac
     1021 accctgatct ccaagggcat cggcggcatc aacctggcgc tgttcaccca gaacaccccg
     1081 ccgccgatgc aggaaggcgg tttgctcaac gccttcttcg gcagcgcggt gatgtgcctg
     1141 atcgcgatcg tcatcggcac cccgctcggc atcgccgccg gcacctggct ggccgagtac
     1201 ggcgccgggc gcaagatggg cacggtggtg cgcttcgtca acgacatcct gctgtcggcg
     1261 ccgtcgatcg tgctgggcct gttcgtctac accctggtgg tgatgcagac cggcggcaac
     1321 ttctcggcgc tggccggcgc gatcgccctg gccttcatcg tgctgccggt ggtggtgcgc
     1381 accaccgacg aaatgctgcg gctggtgccg ccgcagatgc gcgaagcggc gctgtcgctg
     1441 ggcgtgccgc agtggaaggt gatcatgcag gtgctctacc gcagcgcctc ggccggcatc
     1501 gtcaccggcg tgctgctggc gctggcgcgc atcagcggcg aaaccgcgcc gctgctgttc
     1561 accgccttcg gcaaccagta ctggagccac aacgtgctgc agccgatggc gagcgtgccg
     1621 gtggtgatga accagttcgc cggcagcccg tacgaaacct ggcagaccct ggcctgggcc
     1681 ggcgcgctgg tgctgacctt cttcgtcctc gtcgtaagcc tgctcgcgcg cgcgctggtg
     1741 ctgcgcaatc ggatctccaa tgactgacct ctccaccacc cccgccgccc ggaacccggt
     1801 tatgaatgac gctcgtttgt ccgtcgccac gcccagtcgc gaagcggtcg gcgccaatcc
     1861 ggtcaagctg gccgcgcgcg atctgaactt ctattacgac aagttccacg cgctcaagtc
     1921 gatcaacctg gaaatcccgg agaagcaggt caccgcgctg atcggcccgt cgggctgcgg
     1981 caagtcgacc ctgctgcgca tcttcaaccg catctacgcg ctgtacccga agctggaagc
     2041 gcgcggccag gtgctgctgg acggcgacaa catcctcgac ccgaagtacc cgatgaaccg
     2101 cctgcgcagc aaggtcggca tggtgttcca aaagccggtg ccgttcccga tgaccatctt
     2161 cgagaacgtc gcctacggca tccgccacca cgagaagctg tcgaaggcgg aaatgaacga
     2221 ccgcgtcgag cacgcgctgc gccagggcgc gctgtgggac gaggtcaagg acaagctggg
     2281 ccagagcgcg ctgggcctgt ccggcggcca gcagcagcgc ctgtgcatcg cccgcgcggt
     2341 ggcgctgaag ccctcggtgc tgctgctcga cgaaccgacc tcggcgctgg acccgatctc
     2401 caccagccgc atcgagcagc tggtcgagga gctgaagaag gactacacga tcgcgatcgt
     2461 gacccacaac atgcagcagg ccgcgcgcgt gtccgacttc accgccttca tgtacctggg
     2521 cgacctgatc gaacacggcc cgaccgagca gatcttctcc aagccgacca agcagcagac
     2581 cgaggattac atcaccggtc gctttggttg atccggccgg gaatcgggta cggggaatcg
     2641 ggaatcgcaa aagcgattcc cgctccgccc cggacctctc ccgacctcgc aggaccgctt
     2701 ttacgattcc cgattcccca tttcccattc ccggcggtac cgccatgagc aaccagatgc
     2761 acgaccacat cgtcaagagc tacgacgacg agcagcgccg cctgctcgac gaaaccctgc
     2821 gcatgggcga gatcgccgcg gcgcaactgg aatccgcgct cgacgtggtc cagcgccgcg
     2881 acgacaaggc ggccgagcgc atcatcgcca acgacgaggc gatcgacgcg ctggagcagg
     2941 aaatcagcca cgacgtgatg aagctggcgc tgcgcggacc gatggcgcgc gacctgcgcg
     3001 agatcctcgc cgcgatccgc atcgcctccg acatcgagcg cgtcggcgac tacgccgcca
     3061 acgtcgccaa gcgttcgacc gcgctgaacc tgtcgccgcc gctgccgcac gtggccggcc
     3121 tgcacgcgct gggcaccctg gcggtcaagc agttgcgcga cgtgctggtg gcctaccgcg
     3181 acaacgacgt cgagctggcg cagcgggtgc gcgaccgcga cgccgaggtc gacaccgcct
     3241 acaccggcct gttccgcgag ctgctgacct acatgatgga agacgcgcgc agcatcaccg
     3301 cctgcaccca tctgctgttc atggccaaga acatcgagcg cgtcggcgac cacgccacca
     3361 acatcgccga gaacgtgtgg ttcctggtca agggcgacga gccgctgccg ccgcgcgaca
     3421 agcgcgacgt caccagcacc accggcgcgg tctgacccgc cgacgccggc cggcgcaagc
     3481 ttccgccacc ggcaagcttg ccggcccgcc gcgcactgcg cggcgcgccg gcgcgttttc
     3541 gcggttccga tccgcgcagc ctgaacgccg ccgcccgctg caccgcagca tacggcgcag
     3601 gccgcccggc gcggcgccgc ggccagccgc gcgcgcaaac gtcgcaaccg cgcggatgca
     3661 accgttttca acgtccgcgc gctgaatgct ttcttaaccc aggcgcgccg ccgcgccgcg
     3721 gccgcgaacc cttcgtcccc gcccccgcct cttgatggat gcccgcagcc cccggctttg
     3781 ggtgtatttt ccacaagctt aaggaggaca ccggggacat gacgtcgtta ctggccaatg
     3841 gcgatctggc cgccgcccgc gcgggcgaca cggccgcgct ggaacgggtg ctgcggcact
     3901 cgcgccagga cctgcgccgc tacgccgaat tccactgcgt catcaacgac gtcgaggatg
     3961 cggtgcagga gagcctgatc gcggtgtcgc gcaagctgcg cgacctgcgc gtgctcgaat
     4021 gcttcgtctc ctggaccttc cgcatcgtca agcgcgagtg caaccggctc aagcgcatgc
     4081 gccgcatgtt cagcggccag gcgatcaccg aggagatcgc gccggtggtc acgcccgagc
     4141 cggccgaatg gcgccacgac gtcgccgccg cgctggaatc gctgccggcc cattaccgcc
     4201 ggatcatcct gctgcgcgac ctggaaggcc tgaccgtggc cgagatcggc gcggaactgg
     4261 ggctgagccg cgaagcggtg aagtcgcgcc tgcaccgggc gcgggtgctg gcgcgcgagt
     4321 acctgtcgcc gtgactgcat ccgcgccgcg ccgtccgccg tatccctgta tcgaagccgg
     4381 cccgccaccg cggccggccc cggatcccgt accggccagg ccgttacgga tgtcaccgcg
     4441 caccaccccg caaccgttct aagctccgca ccagcaccat ccaaccgggt cccccggggc
     4501 ccagcgcaag accacggcgg agcaatcgcc cgtcgccacc tggagagaac gcatgtccaa
     4561 gaaaatcaac aagcccgtcg ccctcgctct cggcgccacc ctgctcggcg gcctgagcct
     4621 gtcggcttcc gcgttcgcga tgagcgacct gtcgcagggc tacatgctcg gcgcgcagca
     4681 ggcccagacc gccgagaagg ccgccgacgc caaggccacc gacgccaaga aggccgaggg
     4741 cagctgcggc gcgaagaagg ccgaaggcag ctgtggcgcc gacaagaaga aggccgaagg
     4801 cagctgcggc gccgacaaga agaaggccga gggcagctgc ggcggcgaca agaagaaggc
     4861 cgaaggcagc tgcggcggcg acaagaagaa ggccgaaggc aagtgcggcg gcgacaagag
     4921 caagaccgac gccaaggcca agaaggccgg cgaaggcaag tgtggcgaag gcaagtgcgg
     4981 cggttcgatc tgatcgcggc atgacgatgg acggcacgca tccgttgact ccggatgccg
     5041 tcggcctcgg cctgcggcgg gccctgttgg gcccgctgca gtccgcgccg cacgcgcccg
     5101 gcgccgcgcg cgacttcgat ttcctcgaac tcgccccgga gaactggatc ggcgtcggcg
     5161 gccgctacgg cgagcagctg cgcgagctgt cctcgcacta cccgttggtg tgccacggcc
     5221 tgtcgctgtc gctcggcggc accgccgcgc tggacgagac cttcctcgcc cgcgtgcgcc
     5281 gcttcctcga cgaacacggc gtggcctgct acagcgaaca cctgagctac tgcagcgacg
     5341 acggccatct gtacgacctg ctgccgattc cgttcaccga ggaagccgtg cgccacgtcg
     5401 ccgcgcgcat ccgccgcacc caggacatcg tcggccgccg catcgcggtg gagaacgcct
     5461 cctactacgc cgcgccgcac caggaaatga gcgagatcga gttcacccgc gcggtcctgg
     5521 ccgaagccga ttgcgacctg ctgctggacg tcaacaacgt ctacgtcaac tcgatcaacc
     5581 accgctacga cgcgcgcgcg ttcatcgccg cgatgccgca cgagcgcatc gcctatatcc
     5641 acgtcgccgg ccattacgac gaggccgacg acctcaaggt cgacacccac ggcgcgccgg
     5701 tcaagcacga ggtctgggac ctgctcggcg acgcctaccg cctgttcggg ccgcgcccga
     5761 ccctgctgga gcgcgacttc aatttcccgc cctacgccga actggtcgac gaactgggca
     5821 ccgtgcggcg gatcctgcgc gagtcggccg cgccggccgc gcgcgaaccg gcccggcgcg
     5881 cccgcgcctg agcccatgaa cgacgcccct tcgcgcctgc gcgcgcagca gttcgaactg
     5941 acccggcacc tgcgcgaccc cgccgcgagc ccggcgccgg ccgggatcga ggaccgccgc
     6001 ctggcggtct atcgcgagct gctgttcaac aacatcgcga gcctgctgtc gggcaacttc
     6061 ccggcgatcg aacgcatcct cggcgagcaa cgctgggaaa agctggtgcg cgatttctat
     6121 cgcgaccacc gctgccacac gccgctgttc gccgagatcg cgcgcgagtt ccagcgctat
     6181 ctgcaagacc tcgacgaacg cgagccggac ggcctggacc cgccgttcct gcaggaactg
     6241 gcgcattacg aatgggtcga gctggcgctg cagctgagcg aggcgcaggc gccgcacgac
     6301 gacgccggcc acgacccggc cgacctgctg gaccgcgcgc cgcggctgtc gccgctggcc
     6361 tggccgctgg cctatgcctg gccggtgcac cggctcggcc ccgactacca gccggcgcag
     6421 ccgccgccgg cgccgacctt gctgctgctg cggcgcgagg ccgacggcga ggtgcgcttc
     6481 tccgaactca gcccgctcgc gttccggctg ttgcagcgcc tggaagaatc gccgcagctg
     6541 agcgcgcgcg agcagttgcg cgcgctcgcg gccgaggccg gcagcgacga cgaagccggc
     6601 ttcctcgaca tgggcgccgc gctgctgcgg cagatgcgcg ccaacggcgt cgtctacttc
     6661 tgacccgaag ctccctgtag cagcggcgcg agccgcgacc gcgacaacgc aactgcggcg
     6721 caagcctcgg cgcgattgcg tcgtcgcggt cgcggctcgc gccgctcctg caaggggggc
     6781 gccgccgccc ggcgaccgct cagcgtcgat ttgctaggct tcccgccatg atcgaccccg
     6841 gccccgcctc cagcgccgct cctgccgcgc ctgtccccgc caccctcgcc acccgcttcc
     6901 gcggctacct gccggtggtc gtggacgtgg aaaccggcgg attcgactgg aacaagcacg
     6961 ccctgctgga aatcgcggtg cagccgatcg agctggatgc cgacggccgg ctggtgcccg
     7021 gcgaggtcgc cagcgcgcac gtggtgccgg ccgccggcac cctgatcgac cccaagtcgc
     7081 tggaagtcac cggcatcgac atcgaccatc ccttccgcga cgccaagccc gagcggcagg
     7141 cgctggaagc ggtgttcgcg ccggtgcgcg aggcggtcaa gcgccacggc tgccagcgcg
     7201 cgatcctggt cggccacaac gcccacttcg acctcaactt cctcaacgcc gcggtcgcgc
     7261 gcagcggcca caagcgcaac ccgttccacc ccttcagcgt gttcgacacc gtgaccctgg
     7321 ccggcgtggc ctacggccag accgtgctgg cgcgcgcggt gcaggcggcc ggcttcgtct
     7381 ggaacggcga ggaagcgcat tcggcggtgt acgacaccga gcgtaccgcc gaactgttct
     7441 gcaagatcgt caacgcctgg ccggcgcgcg gcgccggcgg ctgagccggc gccgcgcgcg
     7501 ctcatggacg agccctttgc gccgccctac ctgtggatcc ccggtctcgg cgccgatccg
     7561 gatgcgctgc ggcggctgcg cgaacgcgcg ccccggccgc gcgagccgat gggcgaagcc
     7621 tggttcatgg gcggcgaccg ccactactat cgcgggctga tggccggcgc tgcggccgac
     7681 tgggagctgg gcgaactcga aaacgcgttg accgaactga ccagcggccc gacctcgttc
     7741 gatcacctcg acgaatggag cttgtggttc gactacctgc tgccgcgcgc gcaggaacgc
     7801 gccgacgaga cccggcatct gcacgagttg ttggtcagcg cggtatgcgt gcattgcctg
     7861 gacccggccc tgcccggccg ccccgcggag ttccgccgcg acctgctcga taccctgggc
     7921 cgcgcgctga tggcgccgca gcgctggcgc gacggccgca tcgtcgccga ccgcgtgctc
     7981 gatccgctgc gcagctacgt ctgcggctgg accctggaaa gcggcgccgc gttctcggcg
     8041 atctgcagtc tggcgctgcg ctacctggaa acggacgcgg tcgacgcctg gatcggctcg
     8101 ttgctggcga tcgacgatcc gctgtggcgc gcggtcttcg tcgtctggct cgacggcgcc
     8161 gagccgttgc tgttcgaagg ccagcagccc gaacaggcag agcacgaccc gatgaagaac
     8221 atcgcctggg attggtgctg ggtgctgcgc ggcgccgtgc cgtcgccgca actcgatccc
     8281 gaggcgcgcg tgcacgcctt cttcccgccc ggcaacgtcg ccgcgctggc cgcggcgctg
     8341 cggcggcggc tcggcctgga cgcgctgtcg cagtggagcg ccgcgctgcg cgtcgcctgc
     8401 ggggaaacgc gcgatccggg caccgcgctg tggcagttcg agacctccgc gctgcgtgtg
     8461 gccgaacgct tcggcctgga ttgagcgcgc cgcccggccg cgtatcgccg atgcaacatc
     8521 gcgccttggc cgccgccgca gcatcgcttc gttgatccgt gtgtacgcgg cccgatgccg
     8581 cgccgccggc ggccgcagac gctcgcccgc gccggcccac gcagggagaa caggatgttc
     8641 gctttcgacg acgagcagtt tctcgacggc taccgcaaac gcttcggccc gctgcgcgac
     8701 gacctcgccc aggccgtgcg ttttttgatc ggccggatcg agcgcgacgc cagtttcagc
     8761 gccaatccgg tgcatcgcta ccagatcgcg tattgcctgg cgacgttcaa gtgggagacc
     8821 gcgcacaccc tgcggccgat cgacgaattc ggcgatgacg cgcgcttcga gcgcctgtac
     8881 gggccggaca cgcgcatcgg caaggcgctg ggcaacaccc ggcgcggcga cggcgccaag
     8941 taccacggcc gcggctacgt gcagctgacc gggcgcgcca actacgaacg cgccggcaag
     9001 ttcctcggca tcgacctggt cggcaagccc gaggacgcca agcagcccga gatcgcctac
     9061 gccatcgcca tgcagggcat gaagcagggc tggttcaccg gcaagaagct cgaccactat
     9121 ttcaagccgg ggcagctgcc gaactacgaa gaggcgcggc ggatcatcaa cggccaggac
     9181 aaggcccaga ccatcgccga catcgcgcgg cgcttcgacg agttgctggc gaacgcgctg
     9241 gagtgagcgc cgcggcggcc ggcggcgcgc ctgctgcttg ctgcttgccg cttgccgctt
     9301 gccgcttgct gcttgctgct tgctgcttgc aactcagccc gacacacagc ggccggcgcg
     9361 caagagcggc cggcgctgtg gctcaggcgt ccgcgtcgcg cggcaaggcc atgtgaatca
     9421 gcgggaacgg ccgcccctgc ccgtccagcg gcgaacgccc gatgtcgacg aagccgtagc
     9481 gccggtaaaa gccgtgcgcc tgcgggttgt gttcgttgac gtccaccgtc agcgcgccgc
     9541 gcaggccgcg cgcgtggtcg agcaggcgcg tgccgatgcc ctggccgcgg cgcgcgggct
     9601 ccaggaacag catctcgacc tgcgcgtcgg ccaggccgac gaaaccggcg aggccgtcgg
     9661 cgtcttcgaa cacccagatc tcgaccgcgt cgagatagac gtcgcgcacc tgcggacgca
     9721 gcgcgtcgac gtcggcttcg gcgaggaagt cgtgggtggc gcgcaccgag cgttcccaca
     9781 gcgccagcag tcggggattg tcgtcgcggc ggcgttcgcg gatcggcatg gcgcgggtca
     9841 tcgttggcgt cggtggaaac gaaaagcggc gccgctgctg cggcgccgct cggggcgatg
     9901 atggccggtc gccggcgttc tcgccagcgc cgcgccacgg cgcgaatcgc gaacgtccgg
     9961 gcgcaggccg aaggccggcc ctcaggccag cttgaggccg accaggccgg caacgatcag
    10021 cacgatgctg gcgatgcgca gcgcggtggc cggctcgttg aacaggacca tgccgaggat
    10081 caccgtaccg accgcaccga tgccgaccca gatcgcgtag gcggtgccga ccggcaacga
    10141 cttcatcgcc aggccgagca ggccgacgct gatcgccatc gaggcgacgg tgccgaccga
    10201 cggccacagc cgggtgaaac cctcggtgta tttgaggccg atggcccagc cgatttcgaa
    10261 caggccggcg aagacgagaa gaacccaagg catgatgcag ctcccatgat cgatggggcc
    10321 gtccccggat gtaacgcgcg ccgctcgatg ttgggtcgga ctgcgcggac ccgggccgtc
    10381 ccgggtcggt cgcggcgaat tatatcgacg cggccggcgc tggggcctga gcgcgggcct
    10441 tggggtcagc ggggggttac ataggtcttg gcggcgtcat ggcagtgtgg gaatcgctgc
    10501 gccggagcgg aaagcgtcgg gactgaagtc gctcccacaa aagccgagcg tctcccaaag
    10561 gcctcgggcg ggccgatggc ggctgttgtg ggagggactt cagtcccgac gctttccggc
    10621 caaatcgccg cgccccgctc aaccgcgctg ccgcaccgcc tcgaacaggg tcacgccggc
    10681 cgccaccgac acgttcaggc tctccacccc gctggccgca tcgccgccgg gcatcgggat
    10741 cttgaccagt tcgtcgcagg tctcgcgggt cagccggcgc aggccgtcgg cttcgccgcc
    10801 gagcgccagg gcgacgttgc cgcgcaggtc cagcgagtac agcgaagcct gcgcctcgcc
    10861 ggccaggccg tagatccaca cgccgagctt c
//
