LOCUS       NZ_FNNQ01000005        10384 bp    DNA     linear   CON 03-MAY-2024
DEFINITION  Marininema mesophilum strain DSM 45610, whole genome shotgun
            sequence.
ACCESSION   NZ_FNNQ01000005
VERSION     NZ_FNNQ01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Marininema mesophilum
  ORGANISM  Marininema mesophilum
            Bacteria; Bacillota; Bacilli; Bacillales; Thermoactinomycetaceae;
            Marininema.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900106775.1-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 01:02:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,196
            CDSs (total)                      :: 3,130
            Genes (coding)                    :: 3,066
            CDSs (with protein)               :: 3,066
            Genes (RNA)                       :: 66
            rRNAs                             :: 5, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 1 (5S, 16S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 64
            CDSs (without protein)            :: 64
            Pseudo Genes (ambiguous residues) :: 0 of 64
            Pseudo Genes (frameshifted)       :: 21 of 64
            Pseudo Genes (incomplete)         :: 50 of 64
            Pseudo Genes (internal stop)      :: 5 of 64
            Pseudo Genes (multiple problems)  :: 11 of 64
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 02:56:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 30794
            Orig. end                         :: 41178
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNNQ01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10384
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10384
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10384
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5384](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5384
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(393..1049)
                     /gene="ehuD"
                     /locus_tag="BLV90_RS06120"
                     /old_locus_tag="SAMN05444487_10531"
     CDS             complement(393..1049)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuD"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709635.1"
                     /locus_tag="BLV90_RS06120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444487_10531"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="WP_091737888.1"
                     /transl_table=11
                     /translation="MVWDWKYALDILPGLLEVAPVTILATLGAFAVALILGLGLAFARR
                     STNRLLSGVTHAVVEFIRSTPPFVQLFFLFFVMPQFGVTMSALMTGILGLGIHYSTFLS
                     EVYRAGIDSVEKGQWEAATALNFTPRQKWMRIILPQAIRPIIPVMGNYLITLFKETPML
                     LGITVVEMLSQAQIIGSQSFRYTEPVTLVGIFFLVLSYGASLLVKRLEIRLNVHR"
     gene            complement(1053..1682)
                     /gene="ehuC"
                     /locus_tag="BLV90_RS06125"
                     /old_locus_tag="SAMN05444487_10532"
     CDS             complement(1053..1682)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuC"
                     /gene_functions="transport (smcogs) SMCOG1096: polar amino
                     acid ABC transporter, inner membrane"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709636.1"
                     /locus_tag="BLV90_RS06125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444487_10532"
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="WP_342707306.1"
                     /transl_table=11
                     /translation="MKNLLSGAVPTIQLTLLGAALAFLLSFLGGFGRLSRFTAVRGVSG
                     FIVEFFRGTSLLVQLFWLFYVLPFMGIRLESGMVGLLALGFNYGAYGSEVVRSAILSVP
                     QGQHEAGISLNMTSLQRMRYIILPQAFRTMIPSFGNLLIELLKGTALVSLVTIPEMTFE
                     ALAMRTNDAQYTLEIFGWLLVIYFAISYPLTMLMRWTERRFSLGRL"
     gene            complement(1734..2588)
                     /gene="ehuB"
                     /locus_tag="BLV90_RS06130"
                     /old_locus_tag="SAMN05444487_10533"
     CDS             complement(1734..2588)
                     /GO_component="GO:0009897 - external side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0033294 - ectoine binding [Evidence IEA]"
                     /GO_function="GO:0033295 - hydroxyectoine binding [Evidence
                     IEA]"
                     /GO_process="GO:0033308 - hydroxyectoine transport
                     [Evidence IEA]"
                     /GO_process="GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /gene="ehuB"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709637.1"
                     /locus_tag="BLV90_RS06130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444487_10533"
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="WP_245726270.1"
                     /transl_table=11
                     /translation="MVALIFLVTGCGRLQFFSSGESALQQAKDKKSVVIGFANEKPYAY
                     QENGKVTGEAVEIAKKIFKKQGITNIKPVLADFGSLIPGLKAGRFDVITAGMYITPERC
                     KQVTFANPEYNIGEGLAVQKGNPKNIHSYEDIAKKPDSTIAVMKGAIEESYMKDVGVKK
                     SQITIVPDQPTAVSLLDSGRVDGVTMTGPSLLAILKSANAKNVSVVKDFKQPIIDGKEV
                     RGIGAAAFRPEDKDFVKIYNKGLAELKKSNELYETIHPFGFTKADLPGDEKATEICKQP
                     QPK"
     gene            3051..3683
                     /gene="ectA"
                     /locus_tag="BLV90_RS06135"
                     /old_locus_tag="SAMN05444487_10534"
     CDS             3051..3683
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709645.1"
                     /locus_tag="BLV90_RS06135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444487_10534"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_342707307.1"
                     /transl_table=11
                     /translation="MIFLLYKDGDLAINDRRGPSPIKTDDSLRSKATVKKYRHPGNQAV
                     TFRKPTEEDGTAIWQLIKETGVLDLNSSYSYLMLCKYFADTCVIAEEQGDRGVVGFVSA
                     FIQPESPDVIFVWQVAVDHSQRGKGLAKSLLRDLLARDACANVRYLEATISPSNNASQS
                     LFEGLARDIRTNCDTSVCFPEELFPDEGHESEWTYRIGPIETTEEVY"
     gene            3717..4967
                     /gene="ectB"
                     /locus_tag="BLV90_RS06140"
                     /old_locus_tag="SAMN05444487_10535"
     CDS             3717..4967
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-355). E-value: 1e-79.
                     Score: 260.0. Matches aSDomain:
                     nrpspksdomains_BLV90_RS06140_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010897091.1"
                     /locus_tag="BLV90_RS06140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444487_10535"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_091738347.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-82, bitscore:
                     271.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTFETLESEVRSYCRSFPTIFDKARGYKLWDVQGREFIDFFAGA
                     GALNYGHNNPRIKRKLIDYLTDDHVVHSLDMATVAKENFLKKFQDVILAPREMEYKVMF
                     PGPTGANSVESALKLARKVTGRQTVVSFTKGFHGMTLGALSVTGNSFKRHGAGVPLHHA
                     VSMPYDNYHEDVDSLSLLEHYLDDEGSGLDMPAAIILETLQGEGGINVASNHWLQRVEA
                     ICRQRDILLIVDDVQVGCGRTGPFFSFEEAGIQPDIICLSKSIGGYGLPFALTLIKPKL
                     DQWAPGEHNGTFRGHNLAFVASTEALSYWETDELTCETLRKGEIIQRSLSSMTAKHPDT
                     EPEVRGRGLMQGIAFGRKGFAEKVCAIAFERGLIMETSGSDSEVIKVMPPLIIDDEGLN
                     KGLAILDESIQIAKETR"
     aSDomain        3783..4781
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLV90_RS06140_Aminotran_3.1"
                     /evalue="1.00E-79"
                     /label="BLV90_RS06140_Aminotran_3.1"
                     /locus_tag="BLV90_RS06140"
                     /protein_end="355"
                     /protein_start="22"
                     /score="260.0"
                     /tool="antismash"
                     /translation="DKARGYKLWDVQGREFIDFFAGAGALNYGHNNPRIKRKLIDYLTD
                     DHVVHSLDMATVAKENFLKKFQDVILAPREMEYKVMFPGPTGANSVESALKLARKVTGR
                     QTVVSFTKGFHGMTLGALSVTGNSFKRHGAGVPLHHAVSMPYDNYHEDVDSLSLLEHYL
                     DDEGSGLDMPAAIILETLQGEGGINVASNHWLQRVEAICRQRDILLIVDDVQVGCGRTG
                     PFFSFEEAGIQPDIICLSKSIGGYGLPFALTLIKPKLDQWAPGEHNGTFRGHNLAFVAS
                     TEALSYWETDELTCETLRKGEIIQRSLSSMTAKHPDTEPEVRGRGLMQGIAF"
     gene            5001..5384
                     /locus_tag="BLV90_RS06145"
                     /old_locus_tag="SAMN05444487_10536"
     CDS             5001..5384
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709647.1"
                     /locus_tag="BLV90_RS06145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444487_10536"
                     /product="ectoine synthase"
                     /protein_id="WP_091737900.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.4e-53, bitscore:
                     172.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVKHLADLIGTENVVKGETWESRRFLLKKDNVGFSMHETIIKAG
                     TTTNMWYKNHIEAVYCVQGEGEIETLDDETVYPIKDGTIYTLNGHERHQLRAKTELRLI
                     CVFNPPCTGRETHDEDGAYPLLD"
     gene            complement(5754..6329)
                     /locus_tag="BLV90_RS06150"
                     /old_locus_tag="SAMN05444487_10537"
     CDS             complement(5754..6329)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709654.1"
                     /locus_tag="BLV90_RS06150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444487_10537"
                     /product="ECF transporter S component"
                     /protein_id="WP_091737903.1"
                     /transl_table=11
                     /translation="MTKQGSTMKLTTVSILAGIAFLMQYLNFPLPMFPPFLKVDFSELP
                     ALAAGLVFGPLTGILVEFLKNLLHYLFSGSETGIPIGQMANFLAGSIFVVTTVLITRRI
                     QGIKGLIVGFTAASLLMAVIMSVANYWVILPAYTILLNMPIQGHGKSSLVLYGIGPFNL
                     VKGVLTALVFIPLYERIKGRLSLPKVAP"
     regulatory      complement(6488..6623)
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     misc_feature    complement(6656..6669)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 21.62"
                     /tool="antismash"
     gene            complement(6690..8519)
                     /locus_tag="BLV90_RS06155"
                     /old_locus_tag="SAMN05444487_10539"
     CDS             complement(6690..8519)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /locus_tag="BLV90_RS06155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444487_10539"
                     /product="penicillin-binding protein 2"
                     /protein_id="WP_091737906.1"
                     /transl_table=11
                     /translation="MGMVRKHWRSSLVAVVLLFAFVGVLFRLYWIQVFAVHSFSREKAD
                     LISRAQEQQSRDFIVDSGRGAIVDRNGRFLTYDKDWRLIAFPLTDQQLRANQKKLSQVA
                     SILHISYRSLSTQLTSIQRPVALISEAGHDLILSKSQAEKIRKLSLPGIYALRSDNRSG
                     TSLARTIIGRLERNPFLIQDRYPEEWSQGIYDSRSRVGATGLEETFEPFLRGSGGEFLL
                     FGKDGRGRPLNGTTVVKDGGNGSIFPYRVVTTLDADIQQLAERVLRQQKVKDGSVVVQD
                     IASGDVLAMASQPDEETANPNQSPWDNRALMETTPGSIFKTVVTLAALDTGKARMTDVF
                     HCDGSLGRYGLTDTHAHGKQTLTQAYANSCNIAFAQIAEKVGAKTIESYAHRLGLGQPI
                     MWTGELFQEKGFHQLRGEEKGLIFATGTSRKDKGAIAQTGIGQRDVRVTPLQAVNMVTS
                     LFHQGRTPNPRIVKEIQRYDGSLKYTFPDHVLKIKKPIGTRALAEVREMMRKVVTEGTA
                     QSMKTALIPLAGKTGTAQIGVKGDHYNKWMIGYAPYDRPRWSVSVVVRSSRDPKEMRGQ
                     LVFRQVMEGIERLEKEGAKKKAKKTTAEKKKNE"
     gene            8706..9752
                     /locus_tag="BLV90_RS06160"
                     /old_locus_tag="SAMN05444487_10540"
     CDS             8706..9752
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1251: luciferase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017152118.1"
                     /locus_tag="BLV90_RS06160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05444487_10540"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_091737909.1"
                     /transl_table=11
                     /translation="MVKLSVLDQSPIPKGKTAIEALADTRRLAQETEKMGYHRFWVSEH
                     HYSKSLAGSSPEILISHLATITKRMRIGSGGVMLPHYSPYKVAENFKVLEGLYPKRIDV
                     GLGRAPGGLPLSTRALQEGKHNSRDPYPEQLDDLITYLDDRADEQHRFPRLLATPIVES
                     KPEVWLLGSSGGSAHLAARQGAGYAFAQFINGEGGEEVVQYYRDHFQPSSINSTPQTLL
                     AIFVICAESEEEAERQASVLDLTLLLQQKSSSDSRGIPTIEDAERYPYSEWDRMVIRQN
                     RQRMIVGNSEQVKERILQLGRAYSTNEFMLVSIIHDIEAKLRSYQLIAAAFQNDLQQEG
                     IESLTKKE"
     misc_feature    10365..10381
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.58"
                     /tool="antismash"
ORIGIN
        1 gatgaaagtg ctgaaagacc atgccgatat ctccccgcac ctcatgtaag tgcttttcat
       61 ttgccttgac taattctcca ttgacttcct tgtgccataa ctgtcgccca tctacctcaa
      121 tatatccatc cgtaggttcc tccaaggtca tcaacaggcg ggccatggta gttttccctg
      181 aaccactggg tccaatgata gctacgcgtt cccctggata aagatcaaga tcgacctcct
      241 ttaggacatc cagttcacca aaggacttgc tgattttttc aaaacggaca atcggctctt
      301 tcccctcgat tggagcctct gtatcaggcc gctcaatcac ttctgaacca ctcacgtaat
      361 cactcctcct gtatactccc tgattggttt tatcaacgat ggacgttcaa gcgaatctcc
      421 agccgcttta cgaggagcga agctccatag cttaagacga ggaagaagat ccctacgagc
      481 gtaactggtt ctgtatagcg aaagctctgg gaaccaataa tctgagcttg actcaacatc
      541 tccactacgg taatcccgag taacatcggt gtttctttaa ataaggtgat caagtaattg
      601 cccattaccg gaatgatcgg gcggatagcc tgaggtagga tgatccgcat ccatttttgc
      661 cggggggtaa aattaagagc cgtagcagcc tcccactgac ccttttccac tgaatcaatc
      721 cccgctcgat acacctcaga taggaacgtg ctgtaatgaa ttccaagtcc taaaatccct
      781 gtcatcaatg cactcatcgt caccccaaat tgaggcatga caaagaagag aaaaaagagt
      841 tgaacaaagg ggggggttga acggataaat tcaacgacag catgggtcac ccctgacagt
      901 aaacgattgg ttgagcgacg ggcaaaagcc aaacctaacc ctaaaatcag agctacagca
      961 aaagcaccta gggtggctaa tatcgtaaca ggggcaactt ctaagagccc aggtaatata
     1021 tcgagggcat atttccaatc ccagaccact gtttataacc tccctagtga aaatcgacgt
     1081 tcagtccagc gcatcagcat ggttagtgga taagaaatgg caaaataaat cacgagtaac
     1141 catccgaaaa tctctaaggt atactgcgca tcattggttc gcatcgccaa tgcttcaaag
     1201 gtcatctctg ggatggtaac caaggatact aaagcggttc cctttaacaa ttcgatcaac
     1261 agattgccga aggaagggat catcgtacga aatgcctggg ggaggatgat atagcgcatc
     1321 cgttgaagtg acgtcatatt tagtgaaatc ccagcctcat gttgtccttg gggcactgaa
     1381 aggatagcac tgcgtaccac ctcggaacca tacgcaccat aattgaaccc aagggctaag
     1441 agtcctacca tccctgactc taggcgaatc cccataaagg gaagtacata aaacaaccaa
     1501 aatagttgaa ctaataggga cgttccccga aaaaattcaa cgatgaatcc tgacactcca
     1561 cggacagcag taaaacggga caaacgacca aaccccccca gaaaagaaag cagaaatgct
     1621 aaagccgctc ccaacagggt gagttgtatc gtaggaactg ctcctgaaag tagatttttc
     1681 aatatttcaa gatggatctc gatcccaatc ccatcctctc cacgggtata ttgttatttc
     1741 ggttgaggtt gcttacaaat ctctgttgcc ttttcgtccc ctggtagatc tgctttggta
     1801 aatccaaagg ggtgaatcgt ctcatacagc tcattggatt ttttcagttc agcaagacct
     1861 ttgttataga ttttcacaaa atctttatcc tctgggcgga atgcggcagc accaatcccc
     1921 cgcacttctt tcccatcgat gatcggttgc ttgaaatcct ttaccacgga gacatttttc
     1981 gcattggctg acttaagtat ggcaagcaat gaagggccag tcatcgtcac tccatcaaca
     2041 cgtccagaat ccagaagaga tactgccgta ggttgatctg gcacaatcgt aatctgactc
     2101 ttcttcacgc ctacatcttt catataggac tcctcaatcg ctcctttcat tactgcgatt
     2161 gtggaatctg gttttttcgc tatatcttca tagctgtgta tgtttttggg attccctttc
     2221 tgtactgcca acccttctcc aatgttgtat tctggatttg caaacgttac ttgcttacaa
     2281 cgttcaggcg taatatacat ccctgctgta atcacatcaa agcgacctgc tttcaaacca
     2341 ggaatcaggg agccaaagtc agctagcaca ggcttaatgt tggtaatacc ctgtttctta
     2401 aaaattttct tcgcaatctc caccgcttct cctgtcacct tgccattttc ctgataggca
     2461 tagggttttt cattggcaaa tccaatgacc acactcttct tatccttcgc ctgctgaaga
     2521 gccgattcac ctgatgagaa aaactgaagt ctcccacaac ctgttacaag gaaaataagt
     2581 gctaccaatg aaaaagccac tttcttaaac tttaaaatga actttcgcct cctttaaaat
     2641 gattcaattt tcgatagtaa tcattggatt tttttatttt atcatatgcc tccccccata
     2701 cctagataat acctgaaata aacttgaaaa aatcccctat cctttctttt ctttcacaaa
     2761 aaccctagaa tgaaacctat tttcccctat aaaatataga ctaatgaaaa ggagcccttg
     2821 tccttccatc aatcaatatt aagggaaata gaagattata ctgggtatta taccctgaat
     2881 tctccttttg tctgattatg atcagattcg tatctatcca cagaatatgt tcgaatatca
     2941 gccctaagtt gttcagatcc agtcttgtca tgtttaagag tatcctgagc cggtacagta
     3001 ctttcgacgg atgtcttcat tcacgttacg ataaaagacg tatagttttg ttgatatttt
     3061 tattatataa ggacggtgat ctagccatca acgatcgacg gggtccatct ccaataaaaa
     3121 cagatgattc actgagatcg aaagctacag tcaaaaaata tcgtcaccca ggtaatcaag
     3181 cagtcacctt tcgtaaacca accgaagaag acggaacagc aatctggcaa ttgatcaaag
     3241 aaactggtgt attagattta aactccagct acagctatct catgttgtgt aagtattttg
     3301 ctgacacctg tgtcatagca gaagaacagg gggatcgcgg cgtcgttggt tttgtatcgg
     3361 cttttataca accagagtct ccagatgtga tcttcgtatg gcaagtagct gtcgatcact
     3421 cccagcgtgg gaaaggactt gccaaatctc ttctaaggga ccttctcgcc cgggatgcgt
     3481 gtgcgaatgt acgctatttg gaagcgacca tctccccttc taataacgca tcgcaatctt
     3541 tgtttgaagg attggccaga gacattcgta ccaactgtga tacttctgtc tgttttccag
     3601 aagaattatt tccggatgag gggcatgaat cggaatggac ttaccggatc ggccctattg
     3661 aaaccaccga ggaggtttat tgaaacttga ctactatcac cgaagtgaca acagatatga
     3721 gcacatttga aaccctggaa tcggaagtac ggagttactg ccgtagcttt ccaaccattt
     3781 ttgataaagc ccgcgggtat aagctctggg atgtacaagg tcgtgaattt atcgacttct
     3841 tcgccggtgc aggggcttta aattatggtc acaacaaccc gcgcatcaaa cgaaaactga
     3901 tcgactatct taccgacgac catgtggttc atagcctgga tatggccaca gttgcaaagg
     3961 aaaatttctt aaaaaaattt caagacgtta tcctcgctcc acgcgaaatg gaatataaag
     4021 tgatgttccc agggccaaca ggtgccaact ccgtagagag cgcattgaag ttggctcgaa
     4081 aggtaactgg ccgccaaacc gtggttagct tcaccaaagg ctttcatgga atgacactgg
     4141 gagcactctc tgtcacaggg aattcattta aacggcatgg cgctggcgtt cctcttcacc
     4201 atgccgtttc catgccctat gacaattatc atgaggatgt agattccctt agcctccttg
     4261 aacactatct ggatgatgaa ggtagtggac tcgatatgcc tgctgccatc atcctagaga
     4321 cattgcaagg ggaaggtggc attaatgtag ccagtaatca ttggcttcaa agggtagaag
     4381 caatctgtcg tcagagggac atcctattga ttgtcgacga cgtacaggtg ggatgcggtc
     4441 gcacaggacc cttcttcagc tttgaagaag cgggcatcca accagatatt atctgtctct
     4501 ccaaatccat tggggggtat ggtcttccct tcgccctcac attgattaag cccaaactgg
     4561 atcaatgggc tcctggtgag cataatggta ctttccgtgg tcataacttg gcttttgtcg
     4621 cttccacaga agctttatcc tactgggaaa ccgatgaact cacctgtgaa actcttcgta
     4681 aaggagagat catccaacgc tcgttatcct cgatgacagc caagcaccct gacaccgaac
     4741 cggaagtacg gggacgtgga ttgatgcaag gaattgcatt tggacgcaaa ggtttcgcag
     4801 aaaaggtatg tgccatcgcc tttgaacgag gcttgattat ggaaacctca ggttctgaca
     4861 gtgaagtaat caaggtgatg ccacctctca tcattgatga tgaaggtctt aataaaggac
     4921 tcgccatctt agatgaatcg atccaaatag caaaagaaac ccgctaaaaa ataatgtaac
     4981 gaagaaagga tgaacacatc atgatcgtca aacatctagc agacctgatc ggaacagaaa
     5041 acgtagtcaa gggggaaacc tgggaaagcc gtcgctttct cctgaagaag gataatgtcg
     5101 gattctcgat gcatgaaacg attattaaag cgggtaccac cacaaacatg tggtataaga
     5161 accatatcga ggcagtctat tgcgtccaag gtgaaggaga aatcgaaacc cttgatgatg
     5221 aaacggtata tcccatcaag gatggtacga tttacacgtt aaatggccat gagcgtcatc
     5281 aattgcgcgc aaagactgag ctacgtctca tttgtgtctt taatccgcct tgtacagggc
     5341 gtgaaactca tgacgaagat ggtgcatacc ctctactcga ctaaggaagg tattcattgg
     5401 agatatacag accattcaaa tgaatgatca agcaaatttt caatatggaa ctactaagaa
     5461 caagaagtgc tctggcaaca tttcactctt ttccatggcc acatctgcta tagcacgtta
     5521 agcttgcaag caaaaccgca cctaaaaatt aaggtgcggt tttttgcaag ctttgttcta
     5581 tctcatacgt cttaactatt atgaacactt ccataggaaa gaggtgaaaa tacttgcatc
     5641 gcatccccct cgtgcttccc gttattggct atatcatgct gatcgctggg attatcttca
     5701 ccttttatta agaagggaca aaaaaccgac cactagtgta cggtcggtga tgatcatggc
     5761 gccactttag ggagactgag tcttcccttt atccgttcat aaaggggaat aaacaccaaa
     5821 gcagttaaaa caccctttac caggttaaaa ggaccgatac cataaagtac taacgatgat
     5881 tttccatgtc cctgtatcgg catattcagc agaatcgtgt aagcaggtag gatgacccag
     5941 tagttggcca cactcatgat cacagccatc agaagagagg ctgctgtaaa accaacgatc
     6001 aaccctttga tcccttggat tctccttgta atgagaaccg ttgtaaccac aaagatactt
     6061 cctgctagaa agttagccat ttgaccaata ggaatccctg tctcactacc gctaaacaaa
     6121 tagtgcaaca gatttttgag aaattccacc agtattcctg tcagaggacc aaacaccaac
     6181 cccgccgcta aggcaggtaa ttcgctaaag tctactttta aaaagggagg gaacatcggt
     6241 agcggaaaat ttaaatactg catgagaaac gcaatgccag ccaaaataga aacggtggtt
     6301 aacttcattg ttgaaccttg tttcgtcatc agagaacact cctttgtcct tatgtgttct
     6361 gatgacgccg tacagcgaaa aacccgaaaa aaggcaaaga tgccttcctt cgggtttttt
     6421 ctaattggac acttgcaaat aaaggtcagc ctttgtacac agaccccttg catccacacg
     6481 cgtacgtgtc atccttctcc catccagact atactgtcgg tcccagagtt tcaccaggtc
     6541 aaccgcataa gcgggtcacg gactgaggta atcaaggtac catcaccgcc ggtcgggaat
     6601 tacaccctgc cccgaaggat tgaaaacatt attcatttaa atccattgta ctgggcaatc
     6661 aaccgtttgg caagataatg attgaccgtt cattcatttt tcttcttttc tgctgtggtc
     6721 ttttttgctt ttttcttcgc cccttctttt tccaaacgtt ctatgccttc cattacttga
     6781 cgaaaaacca attgacctcg catctcctta ggatctcgag aggaacgtac caccaccgat
     6841 acggaccaac gggggcgatc atagggagca tagccaatca tccatttatt atagtgatct
     6901 ccttttacac cgatctgagc tgtacctgtt ttccctgcta agggtatcaa ggctgttttc
     6961 atcgattggg ccgtcccctc cgtcacaacc ttacgcatca tctcccgaac ctctgcaagg
     7021 gcacgtgtcc caatcggttt ttttatcttc aatacatgat ccggaaacgt gtattttaat
     7081 gatccatcat atctttgaat ttcttttaca atacgcggat tgggggtacg tccctgatga
     7141 aagagggacg ttaccatatt aactgcttgc agtggagtaa cacgaacatc ccgttgacca
     7201 attcccgttt gggcaatagc tcctttatcc ttacgtgatg tccccgtcgc aaagattagc
     7261 cccttttctt ccccacgaag ttgatgaaat cccttttctt gaaacaattc tccggtccac
     7321 atgatgggtt gaccgagccc taaacgatga gcataggatt caatcgtttt tgcacccact
     7381 ttttcagcga tttgagcaaa ggcaatatta catgagttgg catacgcctg tgtgagagtt
     7441 tgtttcccat gggcgtgggt gtctgtcaat ccataacgac ctaggcttcc atcacaatga
     7501 aacacatccg tcatcctagc ctttccagtg tccaaagcgg caagcgtcac tacggtctta
     7561 aaaattgagc ccggtgtggt ttccatcaat gcacggttat cccagggact ttgattgggg
     7621 tttgccgtct cttcgtctgg ttgacttgcc attgccagaa catcgccact tgcgatatct
     7681 tgaaccacca ccgacccatc cttcaccttt tgttgccgca aaacccgctc cgccaactgt
     7741 tgaatgtccg catctagcgt agtaactact cgataaggga agattgatcc atttccccca
     7801 tccttcacta ccgtcgttcc attcagaggt cgaccgcgac cgtctttccc aaagagcaag
     7861 aattctcctc cactgcctcg cagaaagggc tcaaaggttt cctccagtcc cgtcgcccca
     7921 acccgcgacc gagaatcata aataccctgg ctccattctt caggatagcg atcttggatt
     7981 aaaaatggat ttctctctaa tcgtccaatg atcgtgcgcg caagggaggt accactacga
     8041 ttatctgacc gaagagcata gatgcctggt agtgaaagtt tacgaatctt ctctgcctgg
     8101 gatttcgata aaattaagtc atgccccgct tctgaaatta aagccaccgg tcgttgtatg
     8161 gaggttaatt gtgtacttaa tgagcgatag gatatatgta aaatactagc tacttgagat
     8221 aacttcttct gatttgccct caattgttga tctgttagtg gaaatgcgat gagcctccaa
     8281 tccttgtcgt atgttagaaa tcggccattg cgatccacga tagctccccg accactatcc
     8341 actataaagt cccgactttg ttgttcctgg gcgcgggaaa tcaaatccgc cttctcacga
     8401 gaaaacgaat gaactgcaaa gacttgaatc caatacaaac gaaataaaac tcctacaaaa
     8461 gcgaaaagca ataccaccgc caccaagcta ctacgccagt gctttctgac catacccatc
     8521 gtcactcctc ttctcctatc attctcgtca ggaaaagagg gggataaacc gttcttttat
     8581 tttccgttag aatgtggctc tgggatgatt catcctagga ttatccatgt tttgcatttc
     8641 attttgatgg gaatattagt gttcgatctt atagatcgaa atttcttaga cttgaaggtg
     8701 aattcatggt caagttgagt gtactggatc aatctcctat tccaaaagga aaaacggcta
     8761 tcgaagcctt agcagatacc cgtcgattag cacaggaaac cgagaagatg ggataccatc
     8821 gcttctgggt ttccgagcat cattattcaa aaagtttagc gggatcaagt ccagagattc
     8881 tgattagcca cttagcaaca ataacaaagc ggatgcgcat tggctcggga ggcgtgatgc
     8941 ttccccatta cagcccttat aaagtggcag agaacttcaa agtccttgag ggcctctatc
     9001 ctaaacggat cgatgtaggc ttaggaagag ctcctggtgg cctaccccta tccacacgcg
     9061 ctctacagga aggaaagcac aatagtcgag atccttatcc agaacagctc gatgatctaa
     9121 ttacatacct tgatgatcga gcagatgagc aacaccgttt tccacggtta ctcgcaaccc
     9181 ctatcgttga gagcaagcct gaagtatggt tacttggttc tagcggtggc agtgcacatc
     9241 tagccgcccg acaaggggca ggttatgcct ttgctcaatt tattaacggt gagggaggag
     9301 aagaggtcgt ccaatactat cgtgatcatt ttcagccttc ctcaatcaac tcaactcccc
     9361 aaaccctgct ggcgatcttt gtgatttgtg cagagtcgga ggaagaagca gagcggcaag
     9421 cctcagtgtt agacctaacc cttctcctac agcaaaagag ttcgagtgac tcccgaggaa
     9481 tccccactat cgaagatgca gaacgttacc cttattccga gtgggaccgg atggtgatcc
     9541 gtcaaaatcg ccaacggatg atcgttggaa acagtgaaca ggtgaaggaa cgtattcttc
     9601 agttaggaag ggcttatagc accaatgaat ttatgcttgt cagcatcatc cacgatatag
     9661 aagctaaact tcgctcatat caattgatcg ccgctgcctt tcaaaatgac ctacaacaag
     9721 agggaataga gagcttgaca aaaaaagaat aatgttatta aacaacctga gcccggtaga
     9781 ctaccgggtt caggttgttt ataagacgca ctatcgaatg gtcgaaacta cttatgagtt
     9841 acttgctgct tactcttctt aatattttta ttatgtcctg cttctgttcg tgagaaataa
     9901 tgtcgcccac taccatcatt gcgtgctaca tagaagaaat agttattctt ttccggaaac
     9961 aaagctgcct ggagtgccgc atcactagga ttggaaatcg gacctggagg aagccctttg
    10021 gtaaggtatg tattgtacgg gcttttcacc ttgagatcct tatataacag acgtgattta
    10081 tgtttaccgc gagcatactg tactgtggca tccacttgta gttttttacc tactcgcaaa
    10141 cggttttcaa tcactcccgc gatacgggga aattctttac gatcttcacc ttcacgctct
    10201 acaatggagg caatcgttac ccattggtct acggtcagat tacgtttgtc taattgttgc
    10261 tttacttgag gatcttgaag tcgcttggca aattgacgga gcatagcatc cactaattca
    10321 tcgggtttct cttttttagg gagattgtag gtgaacggaa agagataccc ctctagaaga
    10381 tatt
//
