GCF_900106525.1 - 19 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NZ_FNOG01000010.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 2.1	redox-cofactor	9,874	32,090

NZ_FNOG01000008.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 3.1	NRPS-like,NRPS	192,325	240,601

NZ_FNOG01000007.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 4.1	NRPS-like	45,313	86,794
Region 4.2	lanthipeptide-class-ii	227,902	250,778

NZ_FNOG01000022.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 5.1	NRPS	1	2,294

NZ_FNOG01000006.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 6.1	NRPS,RiPP-like	25,720	84,948	High	Le-pyrrolopyrazines	NRPS:Type I
Region 6.2	NRP-metallophore,NRPS	425,482	462,340	Medium	aminochelin/azotochelin/protochelin	NRPS:Type I

NZ_FNOG01000003.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 7.1	terpene-precursor	547,830	568,786
Region 7.2	RiPP-like	638,469	649,353

NZ_FNOG01000005.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 8.1	transAT-PKS,NRPS,PKS-like,T3PKS,lanthipeptide-class-ii	66,818	385,474	High	lysobactin	NRPS:Type I
Region 8.2	RiPP-like	427,290	438,564	Low	lysocin	NRPS:Type I
Region 8.3	NRPS	566,523	610,002

NZ_FNOG01000002.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 12.1	NRPS,T1PKS	558,782	618,217	High	ririwpeptide A/ririwpeptide B/ririwpeptide C	NRPS:Type I
Region 12.2	NRPS-like,NRPS,T1PKS	792,345	854,124

NZ_FNOG01000001.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 15.1	lanthipeptide-class-iii	337,029	359,758

NZ_FNOG01000009.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 16.1	arylpolyene	1	40,469	Low	xanthomonadin I	other:other

NZ_FNOG01000019.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 21.1	NRPS-like	1	17,359	Low	isocomplestatin	NRPS:Type I

NZ_FNOG01000016.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 22.1	NRPS	37,478	81,179

NZ_FNOG01000017.1 (Lysobacter enzymogenes)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 23.1	NRPS	1	43,174	Low	WAP-8294A2	NRPS:Type I

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 2.1	redox-cofactor	9,874	32,090
Region 3.1	NRPS-like,NRPS	192,325	240,601
Region 4.1	NRPS-like	45,313	86,794
Region 4.2	lanthipeptide-class-ii	227,902	250,778
Region 5.1	NRPS	1	2,294
Region 6.1	NRPS,RiPP-like	25,720	84,948	High	Le-pyrrolopyrazines	NRPS:Type I
Region 6.2	NRP-metallophore,NRPS	425,482	462,340	Medium	aminochelin/azotochelin/protochelin	NRPS:Type I
Region 7.1	terpene-precursor	547,830	568,786
Region 7.2	RiPP-like	638,469	649,353
Region 8.1	transAT-PKS,NRPS,PKS-like,T3PKS,lanthipeptide-class-ii	66,818	385,474	High	lysobactin	NRPS:Type I
Region 8.2	RiPP-like	427,290	438,564	Low	lysocin	NRPS:Type I
Region 8.3	NRPS	566,523	610,002
Region 12.1	NRPS,T1PKS	558,782	618,217	High	ririwpeptide A/ririwpeptide B/ririwpeptide C	NRPS:Type I
Region 12.2	NRPS-like,NRPS,T1PKS	792,345	854,124
Region 15.1	lanthipeptide-class-iii	337,029	359,758
Region 16.1	arylpolyene	1	40,469	Low	xanthomonadin I	other:other
Region 21.1	NRPS-like	1	17,359	Low	isocomplestatin	NRPS:Type I
Region 22.1	NRPS	37,478	81,179
Region 23.1	NRPS	1	43,174	Low	WAP-8294A2	NRPS:Type I

No secondary metabolite regions were found in these records:: NZ_FNOG01000023.1 (Lysobacter enzymogenes); NZ_FNOG01000004.1 (Lysobacter enzymogenes); NZ_FNOG01000011.1 (Lysobacter enzymogenes); NZ_FNOG01000013.1 (Lysobacter enzymogenes); NZ_FNOG01000020.1 (Lysobacter enzymogenes); NZ_FNOG01000021.1 (Lysobacter enzymogenes); NZ_FNOG01000012.1 (Lysobacter enzymogenes); NZ_FNOG01000015.1 (Lysobacter enzymogenes); NZ_FNOG01000018.1 (Lysobacter enzymogenes); NZ_FNOG01000014.1 (Lysobacter enzymogenes)

Compact view

NZ_FNOG01000010.1 - Region 1 - redox-cofactor

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Location: 9,874 - 32,090 nt. (total: 22,217 nt) Show pHMM detection rules used

Region on contig edge.

redox-cofactor: (cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

BLU84_RS21955: aminotransferase

smcogs: SMCOG1019: aminotransferase

Hydrolases: amidase back translocase

BLU84_RS21915: amidase

smcogs: SMCOG1105: amidase

BLU84_RS21945: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000936.5	0.41	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0002561.3	0.39	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001444.5	0.36	other (shikimate-derived)	caboxamycin	Streptomyces sp. NTK 937
BGC0001080.5	0.36	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000313.4	0.36	NRPS	beauvericin	Beauveria bassiana
BGC0001221.5	0.36	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0000934.4	0.34	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0002492.3	0.34	NRPS	myxochelin B, myxochelin A	Myxococcus xanthus DK 1622
BGC0002324.3	0.33	NRPS, PKS	myxochelin B, myxochelin N, myxochelin O, myxochelin P, myxochelin Q, myxochelin A	Archangium violaceum
BGC0002015.4	0.33	ribosomal	aborycin	Streptomyces sp. ZS0098

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000936.5	0.41	other (other)	pyocyanine	Pseudomonas aeruginosa PAO1
BGC0002561.3	0.39	other (other)	phenazine SA, phenazine SB, phenazine SC	Streptomyces sp.
BGC0001444.5	0.36	other (shikimate-derived)	caboxamycin	Streptomyces sp. NTK 937
BGC0001080.5	0.36	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000313.4	0.36	NRPS	beauvericin	Beauveria bassiana
BGC0001221.5	0.36	other (phenazine)	marinophenazine A, phenaziterpene A	Streptomyces sp. CNQ-509
BGC0000934.4	0.34	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0002492.3	0.34	NRPS	myxochelin B, myxochelin A	Myxococcus xanthus DK 1622
BGC0002324.3	0.33	NRPS, PKS	myxochelin B, myxochelin N, myxochelin O, myxochelin P, myxochelin Q, myxochelin A	Archangium violaceum
BGC0002015.4	0.33	ribosomal	aborycin	Streptomyces sp. ZS0098

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.35 out of 18.57

Confidence: medium

Additional weak hits (3):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.00 out of 30.97

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.56 out of 29.65

Confidence: weak

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.74 out of 29.72

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: PqqD_RRE; Location: 3 - 92; Score: 103.3

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
RpoN	Regulator of nitrogen assimilation and virulence
ToxT	Regulator of MSHA biosynthesis

NZ_FNOG01000008.1 - Region 1 - NRPS,NRPS-like

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Location: 192,325 - 240,601 nt. (total: 48,277 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

BLU84_RS19670: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: peptidase

BLU84_RS19755: gamma-glutamyltranspeptidase

smcogs: SMCOG1274: gamma-glutamyltranspeptidase

Ligases: ligase synthase

BLU84_RS19720: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU84_RS19725: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU84_RS19815: Pseudouridine synthase

smcogs: SMCOG1154: Pseudouridine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.82	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.76	NRPS	AM-toxin	Alternaria alternata
BGC0000342.4	0.72	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002164.2	0.67	NRPS	peramine	Epichloe festucae
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002164.2	0.67	NRPS	peramine	Epichloe festucae
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.80 out of 26.33

Confidence: medium

Additional weak hits (15):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.03 out of 26.33

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.52 out of 20.97

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.27 out of 18.57

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.39 out of 29.25

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.65 out of 26.12

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.09 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.53 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.08 out of 26.06

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.53 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.70 out of 29.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.42 out of 35.63

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.22 out of 32.93

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.61 out of 20.01

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.45 out of 29.25

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 192324 - 240601:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gln) + (Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 192324 - 234238:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gln) + (Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 194331 - 240601:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gln) + (Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BLU84_RS19720: Gln

Search NORINE for peptide: strict or relaxed

AMP-binding (25..451): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWMTFDASVWELQLFCGGEHNLYGPTEASVQVLT
Nearest Stachelhaus code(s):: Gln DAWQFGLIQK (71% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLU84_RS19725: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (492..894): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFCSGEHNLYGPTEAAIDVSY
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator

NZ_FNOG01000007.1 - Region 1 - NRPS-like

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Location: 45,313 - 86,794 nt. (total: 41,482 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase glycosyltransferase sulfotransferase

BLU84_RS17640: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

BLU84_RS17705: glutathione S-transferase

smcogs: SMCOG1193: glutathione S-transferase

BLU84_RS17710: aminotransferase

smcogs: SMCOG1019: aminotransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.63	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002075.3	0.61	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000307.4	0.61	NRPS	AbT1	Aureobasidium pullulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.63	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002075.3	0.61	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000307.4	0.61	NRPS	AbT1	Aureobasidium pullulans

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.68 out of 26.85

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.24 out of 19.26

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.24 out of 19.26

Confidence: medium

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 27.71 out of 32.74

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 25.37 out of 30.12

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.63 out of 28.19

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (21):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.12 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.27 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.21 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.53 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.87 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.52 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.27 out of 29.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.18 out of 29.25

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 20.59 out of 30.97

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.50 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.98 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.27 out of 27.78

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.29 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.00 out of 29.38

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.61 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.65 out of 24.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.19 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.65 out of 29.65

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.61 out of 20.01

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.65 out of 26.85

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
FuR variant 2	Ferric Uptake Regulator
OsdR	Development and stress management regulator
RutR	Regulator of the pyrimidine and purine metabolism
ZuR variant 1	Zinc-responsive repressor

NZ_FNOG01000007.1 - Region 2 - lanthipeptide-class-ii

Download region GenBank file

Download region SVG

Location: 227,902 - 250,778 nt. (total: 22,877 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase

BLU84_RS18370: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Hydrolases: protease

BLU84_RS18305: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

BLU84_RS18315: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

BLU84_RS18325: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

Lyases: cyclase

BLU84_RS18345: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000622.5	0.49	ribosomal	megacin	Bacillus megaterium
BGC0000504.5	0.45	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0001579.4	0.43	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000554.4	0.43	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000528.5	0.42	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001229.4	0.41	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0000503.5	0.40	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001210.5	0.39	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002698.2	0.39	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001364.3	0.39	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000622.5	0.49	ribosomal	megacin	Bacillus megaterium
BGC0000504.5	0.45	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0001579.4	0.43	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000554.4	0.43	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000528.5	0.42	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001229.4	0.41	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0000503.5	0.40	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001210.5	0.39	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002698.2	0.39	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001364.3	0.39	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BLU84_RS18345

BLU84_RS27270

Leader:

MSDNDVVAA

Core:

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

Core with dehydrations:

WRNEAGDhaDNPAGPLFIGGEFAEADIAGDDhaEAVDhaMIDhbDhaVCysDhbGCysGDhbIHCysCys

antiSMASH-DB 4.0 matches for BLU84_RS27270

100.0%

NZ_FNOG01000007.1:

Lanthipeptides: BLU84_RS27270_lanthipeptide

1

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

48

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

1

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

48

95.8%

NZ_AP014940.1:

Lanthipeptides: LEN_RS25395_lanthipeptide

1

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

48

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEA+SMITSVCTGCGTI CC

1

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAISMITSVCTGCGTIQCC

48

79.2%

NZ_CP072597.1:

Lanthipeptides: J5226_RS01600_lanthipeptide

1

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

48

WRN+AG+DNPAGPLFI GEFAEADIAGD+E +S TS CTGCGTI CC

1

WRNDAGADNPAGPLFISGEFAEADIAGDNEIISRNTSACTGCGTIQCC

48

with 66 more sequence matches

Only the first 10 are displayed here.

54.0%

NZ_CP040709.1:

Lanthipeptides: FF090_RS18940_lanthipeptide

1

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVS-MITSVCTGCGTIHCC

48

WR++A + NPAGPLFIGGE+AEADI GD+ ++S + C+G T CC

2

WRSQADASNPAGPLFIGGEYAEADIIGDTTSISGKCGTACSGSRTRMCC

50

52.1%

NZ_JACHYJ010000001.1:

Lanthipeptides: FHW73_RS02140_lanthipeptide

8

DNPAGPLFIGGEFAEADIAGDSEAVSMITSV----CTGCGTIHC

47

DNPAG LFIGGE+AEADIAG A+++ CTG TI+C

1

DNPAGALFIGGEYAEADIAGGDVAMTVFNCTNCADCTGSITINC

44

51.0%

NZ_JABBWY010000002.1:

Lanthipeptides: HHX37_RS08020_lanthipeptide

1

WRNEA---GSDNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

48

WR+ A G D PAGPLFI GE+AE++I + +S CTG TI CC

1

WRSGADVEGWDCPAGPLFI-GEYAESEITLTALDTRRPSS-CTGANTIPCC

49

50.9%

NZ_JAIQCM010000003.1:

Lanthipeptides: K9V56_RS08295_lanthipeptide

1

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVS-MITSVCTGCGTIHCC

48

WR++A NPAGPLFIGGE+AEADI GD+ +S + C+G T CC

5

WRSQADGSNPAGPLFIGGEYAEADIVGDTSTISGKCGTACSGSRTRMCC

53

48.1%

NZ_CP011130.1and 4 others:

NZ_CP023465.1 Lanthipeptides: CNO08_RS25510_lanthipeptideNZ_CP094357.1 Lanthipeptides: IEQ11_RS02795_lanthipeptideNZ_KQ061218.1 Lanthipeptides: WN54_RS23295_lanthipeptideNZ_LMHM01000017.1 Lanthipeptides: ASD86_RS21560_lanthipeptide

Lanthipeptides: LC55x_RS02840_lanthipeptide

1

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

48

WR +A S+NPAGPLFIGGE+ E+DI G+ S+CTG I+CC

6

WRTDAESNNPAGPLFIGGEYVESDIIGEVAPAITGCSLCTGSMRINCC

53

48.1%

NZ_CP076103.1:

Lanthipeptides: KME82_RS02870_lanthipeptide

1

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

48

WR +A S+NPAGPLFIGGE+ E+DI G+ S+CTG I+CC

6

WRTDAESNNPAGPLFIGGEYVESDIIGEVAPAITGCSLCTGSIRINCC

53

45.8%

NZ_FOZI01000001.1:

Lanthipeptides: BM338_RS00935_lanthipeptide

12

GPLFIGGEFAEADIAGDSEAVSMIT-----SVCTGCGTIHCC

48

GPLF GG+FAEA+I SEA S+ T + CTG T +CC

1

GPLFTGGQFAEAEII--SEASSLTTFGGCGTACTGSHTRYCC

40

44.4%

NZ_CP012109.1:

Lanthipeptides: A176_RS18020_lanthipeptide

1

WRNEAGSDNPAGPLFIGGEFAEADIAGDSEAVS-MITSVCTGCGTIHCC

48

WRN A NPAGPLF+GGEF E+DI ++ +S + C+G T CC

6

WRNSADKSNPAGPLFVGGEFTESDIIHETIVISGRCGTGCSGSQTRQCC

54

39.6%

NZ_CP010407.1and 4 others:

NZ_CP010407.1 Lanthipeptides: SVTN_RS37515_lanthipeptideNZ_CP010407.1 Lanthipeptides: SVTN_RS37515_lanthipeptideNZ_CP010407.1 Lanthipeptides: SVTN_RS37515_lanthipeptideNZ_JACHJY010000017.1 Lanthipeptides: GGE06_RS39715_lanthipeptide

Lanthipeptides: SVTN_RS37515_lanthipeptide

8

DNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

48

DNPAGPL++GG EA + +EA+ S CTG +CC

1

DNPAGPLYVGGAAGEAALTDGTEALLTGCSSCTGSLHSYCC

41

39.6%

NZ_CP029194.1and 1 other:

NZ_CP059991.1 Lanthipeptides: H4W23_RS35675_lanthipeptide

Lanthipeptides: DEJ46_RS04155_lanthipeptide

8

DNPAGPLFIGGEFAEADIAGDSEAVSMITSVCTGCGTIHCC

48

DNPAGPL++GG EA + +EA+ S CTG +CC

1

DNPAGPLYVGGAAGEAALTDATEALLTGCSSCTGSWHSYCC

41

MIBiG 4.0 matches for BLU84_RS27270

14.6%

BGC0000563.4 (AEA03263.1):

Lanthipeptide: venezuelin

30

EAVSMITSVCTGCGTIHC

47

E V ++T + T C I C

4

ECVGLLTLLNTVCIGISC

21

9.8%

BGC0002120.3 (NQE92413.1):

Lanthipeptide: nocathioamide A

28

DSEAVSMITSVCTGCGTIHCC

48

D E+ C ++ CC

29

DDESSRPAAPACHTLSSLPCC

49

allorf_236531_236716

Leader:

MFFCAPRTRTRPMRRRHRARRMNRRDAVHDA

Core:

RLRPPAHRRSPFLSLAHRRCWITTSAPRPW

Core with dehydrations:

RLRPPAHRRDhaPFLDhaLAHRRCysWIDhbDhbDhaAPRPW

antiSMASH-DB 4.0 matches for allorf_236531_236716

100.0%

NZ_AP014940.1and 1 other:

NZ_FNOG01000007.1 Lanthipeptides: allorf_236531_236716_lanthipeptide

Lanthipeptides: allorf_5511339_5511524_lanthipeptide

1

RLRPPAHRRSPFLSLAHRRCWITTSAPRPW

30

RLRPPAHRRSPFLSLAHRRCWITTSAPRPW

1

RLRPPAHRRSPFLSLAHRRCWITTSAPRPW

30

33.3%

NZ_JANAVO010000003.1:

Lanthipeptides: NLX83_RS23610_lanthipeptide

9

RSPFLSLAHRRCWITTSAP

27

RS F + +C ITTSAP

6

RSCF-TCGGGQCGITTSAP

23

23.3%

NZ_LT629701.1:

Lanthipeptides: BLT28_RS40105_lanthipeptide

18

RRCWITTSAP

27

+ C ITTSAP

12

KNCGITTSAP

21

with 2 more sequence matches

20.6%

NZ_JADNMH010000005.1:

Lanthipeptides: I2E99_RS07105_lanthipeptide

18

RRCWITTSAP

27

RRC ITT P

18

RRCTITTECP

27

12.0%

NZ_CP068102.1and 1 other:

NZ_CP068102.1 Lanthipeptides: allorf_1176709_1176894_lanthipeptide

Lanthipeptides: allorf_1176709_1176894_lanthipeptide

6

AHRRSPFLSLAHRRCW

21

+H RSP + RC+

24

SHNRSPISAFGSSRCY

39

allorf_237059_237163

Leader:

MTNTQPCAVAIRGA

Core:

ICMGDLRDSHRGGIQWRKTA

Core with dehydrations:

ICysMGDLRDDhaHRGGIQWRKDhbA

antiSMASH-DB 4.0 matches for allorf_237059_237163

100.0%

NZ_FNOG01000007.1:

Lanthipeptides: allorf_237059_237163_lanthipeptide

1

ICMGDLRDSHRGGIQWRKTA

20

ICMGDLRDSHRGGIQWRKTA

1

ICMGDLRDSHRGGIQWRKTA

20

30.0%

NC_016111.1:

Lassopeptides: SCAT_RS38240_lassopeptide

3

MGDLRDSHRGGIQW

16

GD D H G W

4

WGDYHDWHHGWYGW

17

10.8%

NZ_CP054698.1:

Lanthipeptides: allorf_1882682_1882906_lanthipeptide

10

HRGGIQWRKT

19

HRGG+ W KT

25

HRGGLGWGKT

34

allorf_237381_237575

Leader:

MIRVGDEGHFSACLKTPIGVAAASLRARSLRAG

Core:

SFRPCRPRHRARLAPGRSQVNSIRRDRAADM

Core with dehydrations:

DhaFRPCysRPRHRARLAPGRDhaQVNDhaIRRDRAADM

antiSMASH-DB 4.0 matches for allorf_237381_237575

100.0%

NZ_AP014940.1and 1 other:

NZ_FNOG01000007.1 Lanthipeptides: allorf_237381_237575_lanthipeptide

Lanthipeptides: allorf_5510480_5510674_lanthipeptide

1

SFRPCRPRHRARLAPGRSQVNSIRRDRAADM

31

SFRPCRPRHRARLAPGRSQVNSIRRDRAADM

1

SFRPCRPRHRARLAPGRSQVNSIRRDRAADM

31

29.0%

NZ_BMMP01000005.1:

Lanthipeptides: allorf_160076_160192_lanthipeptide

6

RPRHRARLAPGRSQVNSIR

24

R RHR L P RS + R

4

RARHREHLGPLRSDEEAER

22

allorf_250440_250586

Leader:

MTRSRTKGAPCSALRGICGS

Core:

GPNDNDRWNRATTVRSRAACRRPRRSGA

Core with dehydrations:

GPNDNDRWNRADhbDhbVRDhaRAACysRRPRRDhaGA

antiSMASH-DB 4.0 matches for allorf_250440_250586

100.0%

NZ_FNOG01000007.1:

Lanthipeptides: allorf_250440_250586_lanthipeptide

1

GPNDNDRWNRATTVRSRAACRRPRRSGA

28

GPNDNDRWNRATTVRSRAACRRPRRSGA

1

GPNDNDRWNRATTVRSRAACRRPRRSGA

28

25.0%

NZ_CP012109.1:

Lanthipeptides: allorf_4705695_4705880_lanthipeptide

17

RAACRRP

23

RAACRRP

18

RAACRRP

24

21.4%

NZ_JAOTPO010000006.1:

Lassopeptides: N7Z68_RS10450_lassopeptide

2

PNDNDRWNRATT

13

PND + W+R T+

17

PNDPETWDRFTS

28

with 2 more sequence matches

18.2%

NZ_CP110813.1:

Lanthipeptides: allorf_5693634_5693777_lanthipeptide

1

GPNDNDRWNR

10

G ++DRWNR

22

GCVESDRWNR

31

18.2%

NZ_JAMTCO010000021.1:

Lanthipeptides: allorf_065918_066073_lanthipeptide

5

NDRWNRATTVRSRAACRRPR

24

+WN + R A RRPR

11

GPKWNMTAIHQRRGAFRRPR

30

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.60 out of 19.26

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

Additional weak hits (6):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.64 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.76 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.46 out of 31.73

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.00 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.69 out of 26.06

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.09 out of 30.98

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

BLU84_RS27270 - Class II

Cleavage pHMM score: -3.70

RODEO score: 0

Monoisotopic mass: 4732.1 Da

Molecular weight: 4735.2 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 4753.2 Da N = 1; 4771.2 Da N = 2; 4789.2 Da N = 3

allorf_236531_236716 - Class II

Cleavage pHMM score: -5.30

RODEO score: 0

Monoisotopic mass: 3531.0 Da

Molecular weight: 3533.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3551.1 Da N = 1; 3569.2 Da N = 2; 3587.2 Da N = 3; 3605.2 Da N = 4

allorf_237059_237163 - Class II

Cleavage pHMM score: -4.70

RODEO score: 0

Monoisotopic mass: 2263.1 Da

Molecular weight: 2264.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2282.6 Da N = 1

allorf_237381_237575 - Class II

Cleavage pHMM score: -5.30

RODEO score: 0

Monoisotopic mass: 3577.9 Da

Molecular weight: 3580.1 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3598.1 Da N = 1; 3616.1 Da N = 2

allorf_250440_250586 - Class II

Cleavage pHMM score: -5.20

RODEO score: 0

Monoisotopic mass: 3123.6 Da

Molecular weight: 3125.4 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3143.4 Da N = 1; 3161.4 Da N = 2; 3179.5 Da N = 3

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein

NZ_FNOG01000022.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 2,294 nt. (total: 2,294 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

BLU84_RS25345: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0002135.2	0.65	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001240.4	0.64	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0002135.2	0.65	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001240.4	0.64	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 2294:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asn)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BLU84_RS25345: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (1..363): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YHATFDATVTTAFLVIGGEVNEYGPTETVVGCSI
Nearest Stachelhaus code(s):: Asn DATFVGEVGK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

NZ_FNOG01000006.1 - Region 1 - NRPS,RiPP-like

Download region GenBank file

Download region SVG

Location: 25,720 - 84,948 nt. (total: 59,229 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

BLU84_RS15710: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Ligases: ligase synthase

BLU84_RS15715: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU84_RS15720: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS15745: pseudouridine synthase

smcogs: SMCOG1279: pseudouridine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001650.4	0.72	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0001249.5	0.42	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001135.5	0.41	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.41	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.41	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.41	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.39	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.37	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.37	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.36	NRPS	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001650.4	0.84	NRPS	Le-pyrrolopyrazines	Lysobacter enzymogenes
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.71	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.73 out of 26.85

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.65 out of 18.46

Confidence: strong

Additional weak hits (14):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.71 out of 32.74

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.72 out of 30.22

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.55 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.30 out of 29.38

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.92 out of 36.54

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.83 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.74 out of 27.78

Confidence: weak

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.67 out of 29.72

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.03 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.56 out of 29.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.35 out of 27.78

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.17 out of 35.63

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.45 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 25719 - 84948:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Pro) + (Pro - X) + (X - Pro - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BLU84_RS15710: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (535..956): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVAFQEGHLAVAGEHNHYGPTETHVATAH
Nearest Stachelhaus code(s):: Pro DVQHAAHVAK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS15715: Pro - X

Search NORINE for peptide: strict or relaxed

AMP-binding (31..441): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVAFQEGHLVVAGEHNHYGPTETHVVAAH
Nearest Stachelhaus code(s):: Pro DVQHIAHVVK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1104..1508): X

nrpys: Leu, Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FSQAFDASLIELYMLVGGEVHVYGPTETTVLATA
Nearest Stachelhaus code(s):: Leu or Ile DAIYLGVVLK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS15720: X - Pro - X

Search NORINE for peptide: strict or relaxed

AMP-binding (34..439): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LTEAFDASVLEMLLILGGEVNAYGPTEITVCATQ
Nearest Stachelhaus code(s):: Leu DALLIGAVVK (71% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1099..1507): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVAFQEGHLVVAGEHNHYGPTETHVAVAC
Nearest Stachelhaus code(s):: Pro DVQHAAHVAK (88% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (2183..2587): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAQGFDASVFDMTLLFGGEVNAYGPTEATVCASA
Nearest Stachelhaus code(s):: Leu DAFILGAVCK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
RpoN	Regulator of nitrogen assimilation and virulence
RutR	Regulator of the pyrimidine and purine metabolism

NZ_FNOG01000006.1 - Region 2 - NRP-metallophore,NRPS

Download region GenBank file

Download region SVG

Location: 425,482 - 462,340 nt. (total: 36,859 nt) Show pHMM detection rules used

Region on contig edge.

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU84_RS17390: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: hydrolase protease

BLU84_RS17300: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

BLU84_RS17305: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

BLU84_RS17375: isochorismatase

smcogs: SMCOG1027: isochorismatase

Isomerases: isomerase

BLU84_RS17365: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

BLU84_RS17370: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU84_RS17380: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS27545: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000343.5	0.88	NRPS	enterobactin	Pseudomonas sp. J465
BGC0002135.2	0.59	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.59	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.59	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000357.5	0.59	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001833.4	0.58	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.58	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	0.57	NRPS	AM-toxin	Alternaria alternata
BGC0001240.4	0.57	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001641.4	0.57	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000343.5	0.75	NRPS	enterobactin	Pseudomonas sp. J465
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.63	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000357.5	0.63	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001261.3	0.62	NRPS	AM-toxin	Alternaria alternata
BGC0002474.2	0.62	NRPS	agrobactin	Agrobacterium tumefaciens
BGC0001240.4	0.62	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 24.81 out of 27.55

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.60 out of 19.26

Confidence: medium

Additional weak hits (20):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.83 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.74 out of 28.14

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.39 out of 31.73

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 20.11 out of 36.54

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.36 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.48 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.67 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.26 out of 26.06

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.84 out of 36.54

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.84 out of 26.33

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.29 out of 36.54

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.19 out of 30.98

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.49 out of 28.14

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.91 out of 29.25

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.83 out of 25.88

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 20.29 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.96 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.37 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

BLU84_RS17370: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (35..438): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SESLFPMSSPGGALQIGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PMPAQGVVNK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS27545: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (459..761): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGNQVFGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
GnfM	Regulator of nitrogen-fixation genes
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNOG01000003.1 - Region 1 - terpene-precursor

Download region GenBank file

Download region SVG

Location: 547,830 - 568,786 nt. (total: 20,957 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

BLU84_RS10290: NADH:flavin oxidoreductase/NADH oxidase

smcogs: SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase

Transferases: transferase

BLU84_RS10310: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

Ligases: synthetase

BLU84_RS10275: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002219.2	0.39	terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000639.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000641.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000642.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000638.3	0.37	terpene	carotenoid	Pantoea ananatis
BGC0000640.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000635.3	0.37	terpene	carotenoid	Paracoccus sp. N81106

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002219.2	0.39	terpene	(-)-(R)-nephthenol	Stereum hirsutum FP-91666 SS1
BGC0002398.2	0.38	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000639.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC260
BGC0000641.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC416
BGC0000642.3	0.38	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000638.3	0.37	terpene	carotenoid	Pantoea ananatis
BGC0000640.3	0.37	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000635.3	0.37	terpene	carotenoid	Paracoccus sp. N81106

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

BLU84_RS10275: PT_FPPS_like

Domain 1 (56 - 317): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: IPP, DMAPP → FPP

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.67 out of 19.26

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.35 out of 26.85

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.87 out of 26.85

Confidence: medium

Additional weak hits (6):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.50 out of 26.33

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.81 out of 30.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.22 out of 29.25

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.14 out of 30.22

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.90 out of 29.65

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.80 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
RutR	Regulator of the pyrimidine and purine metabolism

NZ_FNOG01000003.1 - Region 2 - RiPP-like

Download region GenBank file

Download region SVG

Location: 638,469 - 649,353 nt. (total: 10,885 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001778.5	0.30	other (other)	showdomycin	Streptomyces showdoensis
BGC0002046.3	0.27	NRPS, PKS	lobatamide A	Gynuella sunshinyii YC6258
BGC0000188.5	0.27	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0002012.4	0.22	PKS	julichrome Q3-3, julichrome Q3-5	Streptomyces afghaniensis 772
BGC0000344.5	0.20	NRPS	entolysin	Pseudomonas entomophila L48
BGC0000651.5	0.20	terpene	KS-505a	Streptomyces argenteolus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001778.5	0.30	other (other)	showdomycin	Streptomyces showdoensis
BGC0002046.3	0.27	NRPS, PKS	lobatamide A	Gynuella sunshinyii YC6258
BGC0000188.5	0.27	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0002012.4	0.22	PKS	julichrome Q3-3, julichrome Q3-5	Streptomyces afghaniensis 772
BGC0000344.5	0.20	NRPS	entolysin	Pseudomonas entomophila L48
BGC0000651.5	0.20	terpene	KS-505a	Streptomyces argenteolus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.84 out of 26.85

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 21.40 out of 23.05

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.42 out of 20.47

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.64 out of 26.85

Confidence: medium

Additional weak hits (3):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.79 out of 30.22

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.03 out of 29.65

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.72 out of 30.98

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
AfsR	Pleiotropic regulatory for antibiotic production
RutR	Regulator of the pyrimidine and purine metabolism
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNOG01000005.1 - Region 1 - NRPS,PKS-like,T3PKS,lanthipeptide-class-ii,transAT-PKS

Download region GenBank file

Download region SVG

Location: 66,818 - 385,474 nt. (total: 318,657 nt) Show pHMM detection rules used

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
PKS-like: (t2pks2 or ksIII)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

BLU84_RS14045: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

BLU84_RS14135: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

BLU84_RS14235: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

BLU84_RS14405: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BLU84_RS14425: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BLU84_RS26565: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: PPTase acylase acyltransferase kinase methyltransferase

BLU84_RS13995: methyltransferase

smcogs: SMCOG1089: methyltransferase

BLU84_RS14060: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLU84_RS14200: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

BLU84_RS14215: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

BLU84_RS14280: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

BLU84_RS14355: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLU84_RS14360: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

BLU84_RS14370: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLU84_RS26555: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Hydrolases: dehalogenase esterase hydrolase

BLU84_RS14070: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

BLU84_RS14240: thioesterase

smcogs: SMCOG1004: thioesterase

BLU84_RS14400: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

Lyases: cyclase dehydratase hydratase

BLU84_RS14105: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

BLU84_RS14385: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

BLU84_RS14390: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

BLU84_RS14515: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

BLU84_RS26560: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

BLU84_RS14080: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU84_RS14220: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS14225: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS14230: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS14375: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU84_RS14395: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

BLU84_RS14430: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU84_RS14465: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU84_RS14505: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS14510: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS26550: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transport

BLU84_RS14460: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	1.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	1.58	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	1.58	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	1.57	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	1.56	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	1.55	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	1.52	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	1.52	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	1.51	NRPS	AM-toxin	Alternaria alternata
BGC0001641.4	1.41	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000385.5	0.78	NRPS	lysobactin	Lysobacter sp. ATCC 53042
BGC0001758.4	0.77	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.77	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.77	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.77	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002071.2	0.77	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135.2	0.76	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.76	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BLU84_RS14515

BLU84_RS14520

Leader:

MQDNDLIKGWLTGTGNVAGFDNPAGPLFLEGENAVEDAM

Core:

RAPITHSTSVSTCPSGRCICCM

Core with dehydrations:

RAPIDhbHDhaDhbDhaVDhaDhbCysPDhaGRCysICysCysM

antiSMASH-DB 4.0 matches for BLU84_RS14520

100.0%

NZ_AP014940.1and 1 other:

NZ_FNOG01000005.1 Lanthipeptides: BLU84_RS14520_lanthipeptide

Lanthipeptides: LEN_RS12910_lanthipeptide

1

RAPITHSTSVSTCPSGRCICCM

22

RAPITHSTSVSTCPSGRCICCM

1

RAPITHSTSVSTCPSGRCICCM

22

95.5%

NZ_CP067395.1:

Lanthipeptides: JHW38_RS03600_lanthipeptide

1

RAPITHSTSVSTCPSGRCICCM

22

RAPITHSTSVSTCPSGRCICC+

1

RAPITHSTSVSTCPSGRCICCI

22

90.9%

NZ_CP072597.1:

Lanthipeptides: J5226_RS17490_lanthipeptide

1

RAPITHSTSVSTCPSGRCICCM

22

RAPITHSTSVSTCPSGRC+CC+

1

RAPITHSTSVSTCPSGRCVCCI

22

with 8 more sequence matches

86.4%

NZ_CP013140.1and 3 others:

NZ_CP067396.1 Lanthipeptides: JHW41_RS13260_lanthipeptideNZ_CP110813.1 Lanthipeptides: BV903_RS14555_lanthipeptideNZ_FNPT01000003.1 Lanthipeptides: BLV01_RS07955_lanthipeptide

Lanthipeptides: GLE_RS10670_lanthipeptide

1

RAPITHSTSVSTCPSGRCICCM

22

RAPITHSTS+STCP+GRC CCM

1

RAPITHSTSISTCPTGRCYCCM

22

31.8%

NZ_CP018188.1and 1 other:

NZ_VDNV01000006.1 Lanthipeptides: FSV44_RS08680_lanthipeptide

Lanthipeptides: BSR19_RS10820_lanthipeptide

1

RAPITHSTSVSTCPSGRCICC

21

R P +T CP+ +CC

2

RGPGWIATITDDCPNSIFVCC

22

31.8%

NZ_PKIA01000004.1:

Lanthipeptides: CYK22_RS03780_lanthipeptide

1

RAPITHSTSVSTCPSGRCICC

21

R P +T CP+ +CC

2

RGPGWIATITDDCPNSVFVCC

22

31.2%

NZ_CP022753.1:

Lanthipeptides: CDO52_RS03850_lanthipeptide

1

RAPITHSTS--VSTCPSGRCICC

21

R PIT T+ +TCPS + C

10

RGPITLGTTCWGTTCPSANTVSC

32

29.6%

NZ_LT630004.1:

Lanthipeptides: BQ5796_RS18310_lanthipeptide

4

ITHSTSVSTCPSGRC

18

IT + SVS CP+ +C

9

ITVTISVSMCPTTKC

23

26.7%

NZ_FOBE01000018.1:

Lanthipeptides: BMW61_RS21935_lanthipeptide

3

PITHSTSVSTCPSGRC

18

PIT + S + CPS +C

11

PITIAASTAFCPSIKC

26

24.2%

NZ_CP022088.2:

Lanthipeptides: CEQ30_RS41055_lanthipeptide

2

APITHSTSVSTCPSGRC

18

AP+ T+ TCPS C

4

APMVMRTAEGTCPSDDC

20

21.4%

NZ_FNPT01000004.1:

Lanthipeptides: allorf_365518_365742_lanthipeptide

1

RAPITHSTS

9

RAP++HS S

20

RAPVSHSGS

28

MIBiG 4.0 matches for BLU84_RS14520

14.7%

BGC0000535.4 (ADJ56352.1):

Lanthipeptide: nisin A

8

TSVSTCPSG

16

TS+S C G

2

TSISLCTPG

10

BLU84_RS14530

Leader:

MHRIKLIALGVLLAA

Core:

SASTGAATQSQGCFCSTIYYNDAGDTVGVRQPASCGDTGYGTVTKKYKVVAGCVI

Core with dehydrations:

DhaADhaDhbGAADhbQDhaQGCysFCysDhaDhbIYYNDAGDDhbVGVRQPADhaCysGDDhbGYGDhbVDhbKKYKVVAGCysVI

antiSMASH-DB 4.0 matches for BLU84_RS14530

100.0%

NZ_FNOG01000005.1:

Lanthipeptides: BLU84_RS14530_lanthipeptide

1

SASTGAATQSQGCFCSTIYYNDAGDTVGVRQPASCGDTGYGTVTKKYKVVAGCVI

55

SASTGAATQSQGCFCSTIYYNDAGDTVGVRQPASCGDTGYGTVTKKYKVVAGCVI

1

SASTGAATQSQGCFCSTIYYNDAGDTVGVRQPASCGDTGYGTVTKKYKVVAGCVI

55

92.7%

NZ_CP067395.1:

Lanthipeptides: JHW38_RS03590_lanthipeptide

1

SASTGAATQSQGCFCSTIYYNDAGDTVGVRQPASCGDTGYGTVTKKYKVVAGCVI

55

SASTGAATQSQGCFCSTIYYNDAG VGVRQPASCGDTGYG VTKKYKVVAGCV+

1

SASTGAATQSQGCFCSTIYYNDAGAVVGVRQPASCGDTGYGEVTKKYKVVAGCVM

55

92.7%

NZ_CP072597.1:

Lanthipeptides: J5226_RS17500_lanthipeptide

1

SASTGAATQSQGCFCSTIYYNDAGDTVGVRQPASCGDTGYGTVTKKYKVVAGCVI

55

SASTGAATQSQGCFCSTIYYNDAGD VGVRQPASCGD GYG VTKKYKVVAGCV+

1

SASTGAATQSQGCFCSTIYYNDAGDVVGVRQPASCGDPGYGDVTKKYKVVAGCVM

55

with 4 more sequence matches

86.0%

NZ_AP014940.1:

Lanthipeptides: LEN_RS12920_lanthipeptide

1

SASTGAATQS-QGCFCSTIYYNDAGD-TVGVRQPASCGDTGYGTVTKKYKVVAGCVI

55

SASTGAATQS QGCFCSTIYYNDAG VGVRQPASCGDTGYG TK YKVVAGCV+

1

SASTGAATQSAQGCFCSTIYYNDAGTAVVGVRQPASCGDTGYGPTTKNYKVVAGCVM

57

14.5%

NC_020990.1and 9 others:

NC_020990.1 Lanthipeptides: allorf_6768184_6768369_lanthipeptideNZ_CM002273.1 Lanthipeptides: allorf_6788528_6788713_lanthipeptideNZ_CM002273.1 Lanthipeptides: allorf_6788528_6788713_lanthipeptideNZ_CP106650.1 Lanthipeptides: allorf_6778006_6778191_lanthipeptideNZ_CP106650.1 Lanthipeptides: allorf_6778006_6778191_lanthipeptideNZ_CP113103.1 Lanthipeptides: allorf_0072598_0072783_lanthipeptideNZ_CP113103.1 Lanthipeptides: allorf_0072598_0072783_lanthipeptideNZ_MNPQ01000024.1 Lanthipeptides: allorf_033269_033448_lanthipeptideNZ_MNPQ01000024.1 Lanthipeptides: allorf_033269_033448_lanthipeptide

Lanthipeptides: allorf_6768184_6768369_lanthipeptide

6

AATQSQGCFCSTIYYNDAGD

25

AATQ +GC C+ +G

19

AATQVRGCRCARALTRVSGP

38

14.5%

NZ_LIXJ01000004.1:

Lanthipeptides: allorf_208254_208481_lanthipeptide

4

TGAATQSQGCFCSTIY

19

T +AT GCFCS +

5

TTSATCPAGCFCSLLL

20

10.9%

NZ_QGGL01000004.1:

Lanthipeptides: allorf_253363_253503_lanthipeptide

10

SQGCFCSTI

18

SQ CFCS +

3

SQSCFCSIL

11

allorf_365504_365731

Leader:

MPHRLEARSATGG

Core:

SICSGCGMRRFERVFAKKPRPGRKARARIQEAMPPERWRWMRAGRAKRGLSGGQQYMTVSCA

Core with dehydrations:

DhaICysDhaGCysGMRRFERVFAKKPRPGRKARARIQEAMPPERWRWMRAGRAKRGLDhaGGQQYMDhbVDhaCysA

antiSMASH-DB 4.0 matches for allorf_365504_365731

100.0%

NZ_FNOG01000005.1:

Lanthipeptides: allorf_365504_365731_lanthipeptide

1

SICSGCGMRRFERVFAKKPRPGRKARARIQEAMPPERWRWMRAGRAKRGLSGGQQYMTVSCA

62

SICSGCGMRRFERVFAKKPRPGRKARARIQEAMPPERWRWMRAGRAKRGLSGGQQYMTVSCA

1

SICSGCGMRRFERVFAKKPRPGRKARARIQEAMPPERWRWMRAGRAKRGLSGGQQYMTVSCA

62

91.9%

NZ_AP014940.1:

Lanthipeptides: allorf_3261946_3262173_lanthipeptide

1

SICSGCGMRRFERVFAKKPRPGRKARARIQEAMPPERWRWMRAGRAKRGLSGGQQYMTVSCA

62

SICSG GMRRFE VFA+KPRPGR ARARIQEAMPPERWRWMR GRAKRGLSGGQQYMTVSCA

1

SICSGYGMRRFEVVFARKPRPGRNARARIQEAMPPERWRWMREGRAKRGLSGGQQYMTVSCA

62

16.1%

NZ_CP070496.1:

Lanthipeptides: allorf_3787495_3787722_lanthipeptide

20

RPGRKARARIQEAMPPERWRWMRAGRAKR

48

P RAR E +PP R+ G R

4

HPNYAPRARGDEPLPPARYPLCLLGLLSR

32

with 1 more sequence matches

9.7%

NZ_LIWC01000001.1:

Lanthipeptides: allorf_1860455_1860691_lanthipeptide

3

CSGCGMRRF

11

CSGCG+ F

1

CSGCGLEFF

9

MIBiG 4.0 matches for allorf_365504_365731

8.1%

BGC0002150.2 (MBO0654592.1):

Lasso peptide: triculamin

16

AKKPRPGRKARAR

28

+KK +PG R +

1

SKKSKPGDGIRGK

13

allorf_365691_365789

Leader:

MRAALKRGFITDRSPAS

Core:

ACSMHKTLSCTVDRH

Core with dehydrations:

ACysDhaMHKDhbLDhaCysDhbVDRH

antiSMASH-DB 4.0 matches for allorf_365691_365789

100.0%

NZ_AP014940.1and 1 other:

NZ_FNOG01000005.1 Lanthipeptides: allorf_365691_365789_lanthipeptide

Lanthipeptides: allorf_3262133_3262231_lanthipeptide

1

ACSMHKTLSCTVDRH

15

ACSMHKTLSCTVDRH

1

ACSMHKTLSCTVDRH

15

MIBiG 4.0 matches for allorf_365691_365789

7.8%

BGC0002120.3 (NQE92413.1):

Lanthipeptide: nocathioamide A

1

ACSMHKTLSC

10

AC +L C

39

ACHTLSSLPC

48

allorf_366527_366697

Leader:

MRRRLANPSLRRSDGG

Core:

SPRCARGRGREALRRFETRTKTAPRATRPAARARAYSTRQ

Core with dehydrations:

DhaPRCysARGRGREALRRFEDhbRDhbKDhbAPRADhbRPAARARAYDhaDhbRQ

antiSMASH-DB 4.0 matches for allorf_366527_366697

100.0%

NZ_FNOG01000005.1:

Lanthipeptides: allorf_366527_366697_lanthipeptide

1

SPRCARGRGREALRRFETRTKTAPRATRPAARARAYSTRQ

40

SPRCARGRGREALRRFETRTKTAPRATRPAARARAYSTRQ

1

SPRCARGRGREALRRFETRTKTAPRATRPAARARAYSTRQ

40

30.0%

NZ_CP020040.1:

Lanthipeptides: allorf_296719_296913_lanthipeptide

5

ARGRGREALRRFETRTKTAPRATRPA

30

A GRG +A R R AP A PA

10

ADGRGTDAFRPRRGRPGAAPCAGGPA

35

22.5%

NZ_CP050504.1and 3 others:

NZ_CP050504.1 Lanthipeptides: allorf_8953271_8953453_lanthipeptideNZ_CP050504.1 Lanthipeptides: allorf_8953271_8953453_lanthipeptideNZ_CP050504.1 Lanthipeptides: allorf_8953271_8953453_lanthipeptide

Lanthipeptides: allorf_8953271_8953453_lanthipeptide

4

CARGRGREALRRFETRTKTAP

24

C RG GR + R TAP

13

CGRGTGRSSSARVGGAATTAP

33

allorf_370225_370293

Leader:

MPHRARGLAPAYGG

Core:

SEANCGCA

Core with dehydrations:

DhaEANCysGCysA

antiSMASH-DB 4.0 matches for allorf_370225_370293

88.9%

NZ_AP014940.1and 1 other:

NZ_FNOG01000005.1 Lanthipeptides: allorf_370225_370293_lanthipeptide

Lanthipeptides: allorf_3266670_3266738_lanthipeptide

1

SEANCGCA

8

SEANCGCA

2

SEANCGCA

9

allorf_371211_371282

Leader:

MDENDAAA

Core:

RIARAAASVANECAA

Core with dehydrations:

RIARAAADhaVANECysAA

antiSMASH-DB 4.0 matches for allorf_371211_371282

100.0%

NZ_FNOG01000005.1:

Lanthipeptides: allorf_371211_371282_lanthipeptide

1

RIARAAASVANECAA

15

RIARAAASVANECAA

1

RIARAAASVANECAA

15

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.37 out of 29.72

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.75 out of 23.05

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.36 out of 23.79

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 24.10 out of 29.72

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 21.86 out of 27.78

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 23.18 out of 28.19

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.75 out of 23.05

Confidence: medium

Additional weak hits (69):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.74 out of 21.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.76 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.24 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.71 out of 26.06

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.50 out of 26.33

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.35 out of 27.55

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 19.06 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.75 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.39 out of 27.78

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.50 out of 20.97

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.22 out of 20.97

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.04 out of 19.26

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.67 out of 26.33

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.75 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.70 out of 30.25

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.09 out of 20.97

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.01 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.80 out of 21.55

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.75 out of 29.65

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.23 out of 20.01

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.88 out of 25.88

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.57 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.14 out of 29.38

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.04 out of 21.84

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.51 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.44 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.38 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.73 out of 29.38

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.85 out of 25.88

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.57 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.90 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.86 out of 32.74

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.10 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.53 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.31 out of 29.25

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.85 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.92 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.37 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.17 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.74 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.92 out of 26.06

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.39 out of 30.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.05 out of 26.06

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.24 out of 25.88

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.14 out of 31.73

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.81 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.40 out of 29.38

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.53 out of 20.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.85 out of 26.06

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.98 out of 36.54

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.91 out of 19.26

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.09 out of 30.22

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 24.28 out of 30.98

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.51 out of 28.19

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.72 out of 24.78

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.04 out of 29.65

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.00 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.55 out of 28.19

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.00 out of 32.93

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.93 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.72 out of 35.63

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.77 out of 24.78

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.25 out of 25.88

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.87 out of 20.01

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.52 out of 29.65

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.98 out of 29.65

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.96 out of 25.88

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

BLU84_RS14520 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 2183.0 Da

Molecular weight: 2184.6 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 2202.6 Da N = 1; 2220.6 Da N = 2; 2238.6 Da N = 3

BLU84_RS14530 - Class II

Cleavage pHMM score: -4.50

RODEO score: 0

Monoisotopic mass: 5339.6 Da

Molecular weight: 5342.9 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 5360.9 Da N = 1; 5378.9 Da N = 2; 5396.9 Da N = 3; 5415.0 Da N = 4; 5433.0 Da N = 5; 5451.0 Da N = 6; 5469.0 Da N = 7; 5487.0 Da N = 8

allorf_365504_365731 - Class II

Cleavage pHMM score: -2.90

RODEO score: 0

Monoisotopic mass: 7018.6 Da

Molecular weight: 7023.2 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 7041.2 Da N = 1; 7059.3 Da N = 2

allorf_365691_365789 - Class II

Cleavage pHMM score: -7.10

RODEO score: 0

Monoisotopic mass: 1615.8 Da

Molecular weight: 1616.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1634.9 Da N = 1; 1652.9 Da N = 2

allorf_366527_366697 - Class II

Cleavage pHMM score: -5.70

RODEO score: 0

Monoisotopic mass: 4458.5 Da

Molecular weight: 4461.0 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4479.0 Da N = 1; 4497.1 Da N = 2; 4515.1 Da N = 3; 4533.1 Da N = 4; 4551.1 Da N = 5; 4569.1 Da N = 6

allorf_370225_370293 - Class II

Cleavage pHMM score: -5.40

RODEO score: 0

Monoisotopic mass: 735.2 Da

Molecular weight: 735.8 Da

Number of bridges: 1

allorf_371211_371282 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 1454.8 Da

Molecular weight: 1455.6 Da

Number of bridges: 1

Predicted core structure(s)

For candidate cluster 1, location 66817 - 385474:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu - Leu - X - X) + (Leu - Arg - Ile - X - Gly - X - Ser) + (ccmal - ohmal) + (mal) + (mal - mal - ccmal) + (mal - Me-ohmal - ccmal) + (ccmal - ohmal) + (Asp - X) + (D-X - D-Asp - Thr) + (Lys - Pro - X) + (D-Asp - Thr) + (NMe-Thr - Tyr - Gly - ohmal - ohmal - mal) + (Phe - Me-ccmal - ccmal - mal) + (ohmal - Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 66817 - 156312:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NMe-Thr - Tyr - Gly - ohmal - ohmal - mal) + (Phe - Me-ccmal - ccmal - mal) + (ohmal - Me-ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 152119 - 229268:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Asp - X) + (D-X - D-Asp - Thr) + (Lys - Pro - X) + (D-Asp - Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 215444 - 334683:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ccmal - ohmal) + (mal - Me-ohmal - ccmal) + (mal - mal - ccmal) + (mal) + (ccmal - ohmal) + (Leu - Arg - Ile - X - Gly - X - Ser)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 308368 - 385474:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Leu - Leu - X - X) + (Leu - Arg - Ile - X - Gly - X - Ser) + (ccmal - ohmal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BLU84_RS14060: mal

PKS_AT (1..283): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 126.3
Methoxymalonyl-CoA: 42.0
Methylmalonyl-CoA: 39.7
inactive: 38.2
Propionyl-CoA: 32.5
fatty_acid: 23.4
Isobutyryl-CoA: 13.3
Ethylmalonyl-CoA: 7.4
trans-1,2-CPDA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

BLU84_RS26550: Phe

Search NORINE for peptide: strict or relaxed

AMP-binding (479..867): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LVQAFDASVWEMTLIVAGEVNAYGPSETTVCATF
Nearest Stachelhaus code(s):: Phe DAWTIAAVCK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS26555: Thr - Tyr - Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (32..436): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAAHFDFSVWEGNQIFGGEVNMYGITETTVHVTE
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1490..1880): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDASVSETTLVVAGEVNAYGPTETTVCATS
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2547..2942): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: LALTFDIAGLEMQALCGGEWNLYGPTETTIWSTL
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS14215: Asp - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (513..909): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YGVSADLGFTAGKIVTGGEVNHYGPTETTVGILT
Nearest Stachelhaus code(s):: Asp DLTKVGHVGK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1995..2393): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAAHFDFAVWEGNQVFGGEVNMYGITETTVHVSF
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS14220: Lys - Pro - X

Search NORINE for peptide: strict or relaxed

AMP-binding (536..918): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YAAAFDISVEECSLLIGGELNTYGPTEATVAATW
Nearest Stachelhaus code(s):: Lys DNESGGTVAK (76% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1568..1963): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LAQTFDVSFQETFLAVAGEVNHYGPTETHVVTAH
Nearest Stachelhaus code(s):: Pro DVQFAAHVVK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2605..2988): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YAAAFDIAIEEVSLVLGGEINTYGPTETTVVASH
Nearest Stachelhaus code(s):: Gln or Tyr DAESVGTVVK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLU84_RS14225: X - Asp - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (542..940): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HAAAVDTTLEQADLVVGGDINAYGPTEATISSHY
Nearest Stachelhaus code(s):: Arg DTEDIGAITK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2049..2440): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YGVSADLGFTAGKIVTGGEVNHYGPTETTVGILT
Nearest Stachelhaus code(s):: Asp DLTKVGHVGK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3525..3923): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAAHFDFAVWEGNQVFGGEVNMYGITETTVHVSF
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS14230: Asp - X

Search NORINE for peptide: strict or relaxed

AMP-binding (506..896): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YGVAADLGHTAGKIVFGGEFNHYGPTETTVGATC
Nearest Stachelhaus code(s):: Asp DLTKVGHVGK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1636..2028): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAVTFDMSVLECVALLGGDLNGYGPTEASVIVTA
Nearest Stachelhaus code(s):: Ile or Val DFLCLGVVIK (59% 8Å match); Val DALWLGGVFK (41% 8Å match)
Stachelhaus code match:: 70% (weak)

BLU84_RS14355: mal

PKS_AT (8..291): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 47.0
inactive: 30.3
Methoxymalonyl-CoA: 22.1
Methylmalonyl-CoA: 20.0
Isobutyryl-CoA: 17.2
Benzoyl-CoA: 8.5
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Propionyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

BLU84_RS14370: mal

PKS_AT (4..287): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 66.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 98.6
Methoxymalonyl-CoA: 36.6
inactive: 27.3
Methylmalonyl-CoA: 23.8
Propionyl-CoA: 16.1
fatty_acid: 14.7
2-Methylbutyryl-CoA: 10.7
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

BLU84_RS14430: X

AMP-binding (13..362): X

nrpys: 2,3-dohHda

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: KDKLHIGGLNTSPTTYGTEKQTYGLSELGILATQ
Nearest Stachelhaus code(s):: 2,3-dohHda IGNPTGTIMK (68% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLU84_RS14505: Leu - Arg - Ile - X - Gly - X - Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (521..919): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDACTWEPFLLTGGDINNYGPTEATVVATS
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1579..1980): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QALVFDVSVEETNLVIGGDSNAYGPTETTINASV
Nearest Stachelhaus code(s):: Arg DVENVGAINK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2663..3060): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: VDNSFDATTMEGFVLCGGEIHCYGPTETTTYATT
Nearest Stachelhaus code(s):: Ile DAMFLGCTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4178..4581): X

nrpys: Thr, aThr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LNDHFDFTVWEGNQVFGGEVNMYGITETCVHVTF
Nearest Stachelhaus code(s):: Thr or aThr DFWNVGMVHK (100% 8Å match); Thr DFWNVGMVHK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5240..5638): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDIAALELQALIGGEWNMYGPTETTVWSMI
Nearest Stachelhaus code(s):: Gly DILQLGMVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6300..6709): X

nrpys: OH-Asn, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDATVTSLKIVVGGEVNEYGPTETVVGCVV
Nearest Stachelhaus code(s):: OH-Asn or Asn DATKVGEVGK (100% 8Å match); Asn DATKVGEVGK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (7371..7776): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFMCSGEHNLYGPTEAAVDVTA
Nearest Stachelhaus code(s):: Ser DVWHMSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS14510: Leu - Leu - X - X

Search NORINE for peptide: strict or relaxed

AMP-binding (32..425): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDACTWEPFLLTGGDINNYGPTETTVVATS
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1108..1510): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDACTWEPFLLTGGDINNYGPTETTVVATS
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2170..2570): X

nrpys: Ph-Ser, Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDASVFEMTLVVAGDVNAYGPTESTIWATY
Nearest Stachelhaus code(s):: Ph-Ser or Phe DAFTVAAIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3231..3631): X

nrpys: Leu, 3OH-Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDACTWEPFLLTGGDVNNYGPTETTVVATS
Nearest Stachelhaus code(s):: Leu or 3OH-Leu DAWFLGNVVK (100% 8Å match); Leu DAWFLGNVVK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems
ZuR variant 1	Zinc-responsive repressor

NZ_FNOG01000005.1 - Region 2 - RiPP-like

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Location: 427,290 - 438,564 nt. (total: 11,275 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000468.5	0.26	ribosomal	bottromycin A2	Streptomyces bottropensis DSM40262
BGC0001157.6	0.26	ribosomal	bottromycin A2	Streptomyces scabiei 87.22
BGC0001657.4	0.26	NRPS	lysocin	Lysobacter sp. RH2180-5
BGC0000470.6	0.26	ribosomal	bottromycin D	Streptomyces sp. WMMB272
BGC0001145.5	0.25	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000469.6	0.25	ribosomal	bottromycin A2	Streptomyces sp. BC16019
BGC0000604.4	0.20	ribosomal	GE2270A	Nonomuraea sp. WU8817
BGC0000610.5	0.20	ribosomal	nosiheptide	Streptomyces actuosus
BGC0001474.4	0.19	ribosomal	thiopeptin	Streptomyces tateyamensis
BGC0001471.5	0.19	ribosomal	saalfelduracin	Amycolatopsis saalfeldensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000468.5	0.26	ribosomal	bottromycin A2	Streptomyces bottropensis DSM40262
BGC0001157.6	0.26	ribosomal	bottromycin A2	Streptomyces scabiei 87.22
BGC0001657.4	0.26	NRPS	lysocin	Lysobacter sp. RH2180-5
BGC0000470.6	0.26	ribosomal	bottromycin D	Streptomyces sp. WMMB272
BGC0001145.5	0.25	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000469.6	0.25	ribosomal	bottromycin A2	Streptomyces sp. BC16019
BGC0000604.4	0.20	ribosomal	GE2270A	Nonomuraea sp. WU8817
BGC0000610.5	0.20	ribosomal	nosiheptide	Streptomyces actuosus
BGC0001474.4	0.19	ribosomal	thiopeptin	Streptomyces tateyamensis
BGC0001471.5	0.19	ribosomal	saalfelduracin	Amycolatopsis saalfeldensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.55 out of 18.46

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

Additional weak hits (5):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.75 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.35 out of 29.38

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.24 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNOG01000005.1 - Region 3 - NRPS

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Location: 566,523 - 610,002 nt. (total: 43,480 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU84_RS15370: nucleotide sugar dehydrogenase

smcogs: SMCOG1152: nucleotide sugar dehydrogenase

Hydrolases: esterase

BLU84_RS15325: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase

BLU84_RS15330: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.65	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001166.5	0.65	NRPS	HC-toxin	Alternaria jesenskae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.58 out of 23.79

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.80 out of 28.19

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

Additional weak hits (14):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.40 out of 26.33

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.58 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.35 out of 29.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.60 out of 21.84

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.08 out of 18.57

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 21.71 out of 33.73

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.86 out of 32.74

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.97 out of 24.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.60 out of 30.98

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.85 out of 18.57

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.59 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.69 out of 30.98

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 8, location 566522 - 610002:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BLU84_RS15330: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (571..966): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LEPAFDASTFEAWLLAGGDVNGYGPTENSTFTTV
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism
ZuR variant 1	Zinc-responsive repressor

NZ_FNOG01000002.1 - Region 1 - NRPS,T1PKS

Download region GenBank file

Download region SVG

Location: 558,782 - 618,217 nt. (total: 59,436 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU84_RS06755: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Hydrolases: esterase hydrolase

BLU84_RS06775: thioesterase

smcogs: SMCOG1004: thioesterase

BLU84_RS06855: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: dehydratase

BLU84_RS06820: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase synthase

BLU84_RS06780: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU84_RS06785: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.90	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.89	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.89	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.87	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.85	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.81	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.80	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.79	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001166.5	0.76	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.74	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.75	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.74	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 24.49 out of 30.22

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.39 out of 23.79

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.85 out of 20.97

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.86 out of 18.57

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.86 out of 18.57

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.46 out of 28.19

Confidence: medium

Additional weak hits (14):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.22 out of 31.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.64 out of 21.55

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.42 out of 28.14

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.54 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.60 out of 30.25

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.42 out of 33.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.93 out of 27.78

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.57 out of 30.98

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.04 out of 27.04

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.02 out of 24.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.00 out of 28.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.32 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.27 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 558781 - 618217:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ccmal) + (Ser - His - Ser - Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BLU84_RS06780: Ser - His - Ser - Val

Search NORINE for peptide: strict or relaxed

AMP-binding (523..921): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEATVDVTA
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1605..2012): His

nrpys: His

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SVRSFDSSVAGGLCIVAGEYNEYGPTEATVWASV
Nearest Stachelhaus code(s):: His DSALIAEVWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2693..3091): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDLSVWEWHFICGGEHNLYGPTEATLDVTA
Nearest Stachelhaus code(s):: Ser DVWHISLVDK (88% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (3789..4199): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGNSFDMTTWEGMLLVGGDWNVYGPTETTVIATA
Nearest Stachelhaus code(s):: Leu DMHMLGVIIK (53% 8Å match)
Stachelhaus code match:: 80% (moderate)

BLU84_RS06785: mmal

PKS_AT (546..843): mmal

ATSignature: Malonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Malonyl-CoA: 66.7%; Methylmalonyl-CoA: 66.7%; CE-Malonyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 115.4
Ethylmalonyl-CoA: 107.0
Methoxymalonyl-CoA: 102.9
Malonyl-CoA: 91.1
Isobutyryl-CoA: 67.9
trans-1,2-CPDA: 65.2
Propionyl-CoA: 57.4
Acetyl-CoA: 54.6
2-Methylbutyryl-CoA: 49.3
CHC-CoA: 35.1
Benzoyl-CoA: 27.2
fatty_acid: 27.1
inactive: 25.7
3-Methylbutyryl-CoA: 15.8

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
ClgR	Caseinolytic protease gene regulator
IolR	Regulator of the inositol catabolism
ToxT	Regulator of MSHA biosynthesis
ZuR variant 1	Zinc-responsive repressor

NZ_FNOG01000002.1 - Region 2 - NRPS,NRPS-like,T1PKS

Download region GenBank file

Download region SVG

Location: 792,345 - 854,124 nt. (total: 61,780 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

BLU84_RS07620: NADH:flavin oxidoreductase/NADH oxidase

smcogs: SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase

BLU84_RS07660: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BLU84_RS07690: Dioxygenase TauD/TfdA

smcogs: SMCOG1046: Dioxygenase TauD/TfdA

Transferases: acylase

BLU84_RS07715: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

BLU84_RS07625: thioesterase

smcogs: SMCOG1004: thioesterase

BLU84_RS07665: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase

BLU84_RS07655: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU84_RS07705: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU84_RS07710: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS07720: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	1.55	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	1.52	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	1.51	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	1.51	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	1.47	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	1.37	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001825.4	1.36	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000307.4	1.33	NRPS	AbT1	Aureobasidium pullulans
BGC0000342.4	1.32	NRPS	enniatin	Fusarium equiseti
BGC0001641.4	1.31	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.74	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.74	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001133.4	0.71	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.81 out of 27.55

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 25.32 out of 29.72

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.31 out of 23.79

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

Additional weak hits (15):

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.03 out of 19.26

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.58 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.30 out of 30.25

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.80 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.11 out of 25.88

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.85 out of 30.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.46 out of 26.06

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.05 out of 39.62

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.88 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.61 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.04 out of 20.01

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.04 out of 29.65

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 792344 - 854124:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Asn - ohmal) + (X) + (D-Asn - Asp) + (Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 792344 - 835326:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Asn - Asp) + (Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 808700 - 854124:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Asn - ohmal) + (X) + (D-Asn - Asp) + (Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 831750 - 854124:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (Asn - ohmal) + (X) + (D-Asn - Asp)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BLU84_RS07655: Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (399..794): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAVHFDFSVWEGSQVFGGEINMFGITETTVHVTA
Nearest Stachelhaus code(s):: Thr DFWSVGMVHK (94% 8Å match); aThr DFWSVGMVHK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS07705: Asn - Asp

Search NORINE for peptide: strict or relaxed

AMP-binding (541..949): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YHCSFNATITTTFLVIGGEVNEYGPTETVVGCCT
Nearest Stachelhaus code(s):: Asn DATFVGEVGK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (2063..2454): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: LGMAFDASLQGHDCVIGGEFNAYGPTECTVNASM
Nearest Stachelhaus code(s):: Tyr DAIDVGAVGK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

BLU84_RS07710: X

AMP-binding (1208..1611): X

nrpys: OH-Asn, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWATFDATVTSLKIVVGGEVNEYGPTETVVGCSV
Nearest Stachelhaus code(s):: OH-Asn or Asn DATKVGEVGK (94% 8Å match); Asn DATKVGEVGK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS07715: Asn - mal

Search NORINE for peptide: strict or relaxed

AMP-binding (532..940): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YHASFDATITSAKIIIGGEVNEYGPTETVVGCSV
Nearest Stachelhaus code(s):: Asn DATKIGEVGK (68% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1671..1965): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 128.2
Methoxymalonyl-CoA: 59.8
inactive: 54.2
Methylmalonyl-CoA: 53.0
Propionyl-CoA: 42.3
Ethylmalonyl-CoA: 23.0
2-Methylbutyryl-CoA: 21.2
Isobutyryl-CoA: 20.9
CHC-CoA: 15.1
fatty_acid: 13.8
Acetyl-CoA: 12.4
Benzoyl-CoA: 11.6
trans-1,2-CPDA: 8.7
3-Methylbutyryl-CoA: 0.0

BLU84_RS07720: X

AMP-binding (180..588): X

nrpys: Gln, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YHATFDATVTTAKLVIGGEVNEYGPTETVVGCSI
Nearest Stachelhaus code(s):: Gln or Asn DATKVGEVGK (79% 8Å match); OH-Asn or Asn DATKVGEVGK (76% 8Å match); Asn DATKVGEVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
GnfM	Regulator of nitrogen-fixation genes
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C

NZ_FNOG01000001.1 - Region 1 - lanthipeptide-class-iii

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Location: 337,029 - 359,758 nt. (total: 22,730 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

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Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU84_RS01460: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: sulfotransferase

BLU84_RS01465: Sulfotransferase domain

extras: Sulfotransfer_3: Sulfotransferase domain

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000551.5	0.52	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000563.4	0.52	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0002700.2	0.51	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000513.3	0.49	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000518.3	0.44	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002698.2	0.44	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000546.3	0.40	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0002674.2	0.40	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0001862.4	0.40	ribosomal	pallidocin	Aeribacillus pallidus
BGC0000487.5	0.40	ribosomal	carnocyclin	Carnobacterium maltaromaticum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000551.5	0.52	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000563.4	0.52	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0002700.2	0.51	ribosomal	bacinapeptin	Bacillus nakamurai
BGC0000513.3	0.49	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000518.3	0.44	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002698.2	0.44	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000546.3	0.40	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0002674.2	0.40	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0001862.4	0.40	ribosomal	pallidocin	Aeribacillus pallidus
BGC0000487.5	0.40	ribosomal	carnocyclin	Carnobacterium maltaromaticum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.29 out of 27.55

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

Additional weak hits (2):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.00 out of 18.57

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.06 out of 28.19

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
GnfM	Regulator of nitrogen-fixation genes
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNOG01000009.1 - Region 1 - arylpolyene

Download region GenBank file

Download region SVG

Location: 1 - 40,469 nt. (total: 40,469 nt) Show pHMM detection rules used

Region on contig edge.

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU84_RS20395: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: deacetylase hydrolase

BLU84_RS20290: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

BLU84_RS20310: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

BLU84_RS20355: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: synthase

BLU84_RS20375: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU84_RS20385: polyprenol-monophosphomannose synthase ppm1

smcogs: SMCOG1123: polyprenol-monophosphomannose synthase ppm1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000840.5	0.56	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0000867.3	0.49	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002510.2	0.44	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0001987.2	0.43	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0001928.2	0.43	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002008.3	0.43	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0002138.2	0.43	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0001197.5	0.42	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000837.4	0.42	other (other)	APE Vf	Aliivibrio fischeri ES114
BGC0002712.2	0.41	PKS	WS-5995 D, WS-5995 B, WS-5995 C, SEK 43	Streptomyces yanglinensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000840.5	0.56	other (other)	xanthomonadin I	Xanthomonas oryzae pv. oryzae
BGC0000867.3	0.49	other (other)	polyhydroxyalkanoic acids	Ectothiorhodospira shaposhnikovii
BGC0002510.2	0.44	PKS	youssoufene A1, youssoufene B1, youssoufene B2, youssoufene B3, youssoufene B4	Streptomyces sp.
BGC0001987.2	0.43	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0001928.2	0.43	other (other)	(2R,3S,4S)-5-fluoro-2,3,4-trihydroxypentanoic acid	Streptomyces sp. MA37
BGC0002008.3	0.43	other (other)	aryl polyenes	Xenorhabdus doucetiae
BGC0002138.2	0.43	PKS	fuelimycin A, fuelimicin B, fuelimicin C	Streptomyces albireticuli NRRL B-1670
BGC0001197.5	0.42	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0000837.4	0.42	other (other)	APE Vf	Aliivibrio fischeri ES114
BGC0002712.2	0.41	PKS	WS-5995 D, WS-5995 B, WS-5995 C, SEK 43	Streptomyces yanglinensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.48 out of 23.79

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.67 out of 20.97

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.58 out of 20.97

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.19 out of 19.26

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 18.08 out of 25.31

Confidence: medium

Additional weak hits (18):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.58 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.17 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.51 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.98 out of 26.06

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.03 out of 19.26

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.24 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.10 out of 27.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.23 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.44 out of 28.14

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.84 out of 27.78

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.15 out of 30.97

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.57 out of 28.19

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.88 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.98 out of 32.93

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.87 out of 29.25

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.76 out of 21.84

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.73 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CatR	H2O2-responsive repressor
CcpA	Catabolite control protein A
IolR	Regulator of the inositol catabolism

NZ_FNOG01000019.1 - Region 1 - NRPS-like

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Location: 1 - 17,359 nt. (total: 17,359 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU84_RS25245: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BLU84_RS25260: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: aminotransferase methyltransferase

BLU84_RS25265: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

BLU84_RS25275: methyltransferase

smcogs: SMCOG1089: methyltransferase

Ligases: ligase

BLU84_RS25270: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU84_RS28030: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.62	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.61	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.61	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.59	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.59	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.59	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.58	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002274.2	0.58	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001135.5	0.63	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002274.2	0.63	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 18.15 out of 18.46

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.07 out of 20.97

Confidence: medium

Additional weak hits (1):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.35 out of 32.74

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 17359:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BLU84_RS25270: X

AMP-binding (10..393): X

nrpys: Gly, Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDVSQWESFVFCAGEVDYYGPTEATIFATA
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (59% 8Å match); Ala DVWCIAAIYK (56% 8Å match); cohOhi DVHIIAYIVK (53% 8Å match)
Stachelhaus code match:: 60% (weak)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FNOG01000016.1 - Region 1 - NRPS

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Download region SVG

Location: 37,478 - 81,179 nt. (total: 43,702 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

BLU84_RS25125: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS25130: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS25140: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU84_RS27420: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.74	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001133.4	0.69	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0000307.4	0.69	NRPS	AbT1	Aureobasidium pullulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.74	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001133.4	0.69	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0000307.4	0.69	NRPS	AbT1	Aureobasidium pullulans

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

Additional weak hits (8):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.81 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.00 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.12 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.01 out of 30.25

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.85 out of 32.74

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.04 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.97 out of 24.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.66 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 37477 - 81179:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Tyr - X) + (Hpg - Cys) + (Gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BLU84_RS25125: X - Tyr - X

Search NORINE for peptide: strict or relaxed

AMP-binding (467..863): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGFSFDISIVEGQTVSAGEWNGYGPTEATVYSLV
Nearest Stachelhaus code(s):: Ala DIVQLGLVYK (59% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1522..1920): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDAAVSETTLISAGEINAYGPSESTVCASI
Nearest Stachelhaus code(s):: Tyr DASTIAAVCK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2587..2997): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWYGFDMTQKNANCALGGELNMYGPSECTDIAIA
Nearest Stachelhaus code(s):: Hse DLKNVGSDVK (65% 8Å match)
Stachelhaus code match:: 60% (weak)

BLU84_RS25130: Hpg - Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (538..925): Hpg

nrpys: Hpg

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YCYAHDAAVLDAHSVFGGEANLYGSSEATLVSLH
Nearest Stachelhaus code(s):: Hpg DALHLGLLCK (44% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1603..2002): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDISFDLSVYDGNSMMSGDYSLGGATEASIWSVG
Nearest Stachelhaus code(s):: Cys DLYNMSLIWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS27420: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (532..923): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDISILECQCMVGGEINLYGPTETAIWSSY
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NZ_FNOG01000017.1 - Region 1 - NRPS

Download region GenBank file

Download region SVG

Location: 1 - 43,174 nt. (total: 43,174 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

BLU84_RS25150: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002384.3	0.74	NRPS	lysocin E	Lysobacter sp.
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001657.4	0.72	NRPS	lysocin	Lysobacter sp. RH2180-5
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001133.4	0.71	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002384.3	0.74	NRPS	lysocin E	Lysobacter sp.
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001657.4	0.72	NRPS	lysocin	Lysobacter sp. RH2180-5
BGC0001833.4	0.72	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001133.4	0.71	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.70	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001166.5	0.70	NRPS	HC-toxin	Alternaria jesenskae
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 43174:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Arg - Ser - Gly - D-NMe-Phe - Leu - D-Arg) + (Glu - D-Gln - D-Trp - Ile)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BLU84_RS25145: Glu - Gln - Trp - Ile - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (462..864): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFICGGEHHSYGPTETSIGVAA
Nearest Stachelhaus code(s):: Glu DVWHIGSIGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1517..1924): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: HWMTFDAAQWELQAFCGGEVNLYGPTETTIQVVA
Nearest Stachelhaus code(s):: Gln DAWQFGLIQK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3038..3439): Trp

nrpys: Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AARPFDASLAAGISILGGEYNEYGPTETAIWCAS
Nearest Stachelhaus code(s):: Trp DAAIIGEIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4569..4963): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDRSFDASTYEVFLWSGGEVHVYGPTETTTFATC
Nearest Stachelhaus code(s):: Ile DAYFWGVTFK (100% 8Å match); Ile or Val DAYFWGVTFK (97% 8Å match); Val DAYFWGVTFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5615..5917): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGNQVFGGEVNMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU84_RS25150: Arg - Ser - Gly - Phe - Leu - Arg

Search NORINE for peptide: strict or relaxed

AMP-binding (752..1159): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LHQAFDTAIEGWDLILGGEYNAYGPTEYAVDAAC
Nearest Stachelhaus code(s):: Arg DTEDIGAVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2270..2681): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAVDVTA
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3332..3731): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FAMTFDIAGLELQALCGGEWNLYGPTETTIWSLT
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4396..4789): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDAAFWEMTLIVAGETNAYGPTETTVCATM
Nearest Stachelhaus code(s):: Phe DAWTIAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6332..6733): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSQAFDAAAMEMLIIVGGEVNAYGPTEITICAAM
Nearest Stachelhaus code(s):: Leu DAMLIGAICK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (7391..7800): Arg

nrpys: Arg

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LEHAFDTSLEGWDLVLGGEYNCYGPTECTVDASY
Nearest Stachelhaus code(s):: Arg DTEDVGCVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor