LOCUS       NZ_FNOG01000022         2294 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Lysobacter enzymogenes strain ATCC 29487, whole genome shotgun
            sequence.
ACCESSION   NZ_FNOG01000022
VERSION     NZ_FNOG01000022.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lysobacter enzymogenes
  ORGANISM  Lysobacter enzymogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Lysobacter.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900106525.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 20:30:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,091
            Genes (coding)                    :: 5,036
            CDSs (with protein)               :: 5,036
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 8 of 55
            Pseudo Genes (incomplete)         :: 49 of 55
            Pseudo Genes (internal stop)      :: 4 of 55
            Pseudo Genes (multiple problems)  :: 6 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:36:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2294
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNOG01000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2294
                     /db_xref="taxon:69"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0111606_122"
                     /organism="Lysobacter enzymogenes"
                     /strain="ATCC 29487"
                     /type_material="type strain of Lysobacter enzymogenes"
     gene            <1..>2294
                     /locus_tag="BLU84_RS25345"
                     /old_locus_tag="SAMN05421681_1221"
     region          1..2294
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2294
                     /SMILES="NC(CC(=O)N)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2294
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>2294](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>2294
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>2294
                     /NRPS_PKS="Domain: AMP-binding (1-363). E-value: 3.1e-78.
                     Score: 255.1. Matches aSDomain:
                     nrpspksdomains_BLU84_RS25345_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (472-542). E-value: 9e-25. Score:
                     78.7. Matches aSDomain: nrpspksdomains_BLU84_RS25345_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (560-764). E-value:
                     2.4e-67. Score: 219.0. Matches aSDomain:
                     nrpspksdomains_BLU84_RS25345_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="BLU84_RS25345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_1221"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_139175256.1"
                     /sec_met_domain="AMP-binding (E-value: 3e-91, bitscore:
                     292.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.9e-19, bitscore:
                     56.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.2e-49, bitscore:
                     155.1, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVGLHVGRSLEQVVGVLAILKAGGAYLPLEPEHPNARLAYMCSD
                     SGVRTILSVGELAERCATWGEWTLSLDDEATLANYPSNTPEVAGLTPQHLAYVIYTSGS
                     TGQPKGVLVPHGGVVNYLDHAQTYLQPHHRGAVMGTPLSFDATVTTLFAPLLAGKRLIV
                     LPSEAGALFAGLSHYLFDDGHDWLFKLTPAHLDGLYANYPAHSGPSARRHCLVIGGEQL
                     TTATVAKWQRDRLPNAEYVNEYGPTETVVGCSIYTVRTPADLARCGHAVAIGRPIQNTR
                     LYVLNELDEPVPAGAVGELCIGGDGVTRGYQNLDALTASRFREHRFGQGAVERLYHSGD
                     LVRCGHDGELEYLGRRDEQVKLRGYRIELGEIESQLKRDARVSDAAAAIQGEGNDRRLV
                     AFVASNEEETAHAALVDELQQALRQSLAEFMRPSAIAVLASLPLTANGKVDRAALPSLG
                     GAVAAAYRAPETETERVLATLWQELLERSEPVGAEDNFFRLGGHSLLATRMIVQINQRY
                     GLDLSVKDAFERQSLAALAEWVGQRRSDARPVAGAIVARTEAGPAPLSYAQQRLWFIDR
                     LSGNSRQYNIAMPIAIQGRLDADALTAALRTIVQRHEVLRTVYREDTTGTVLQHALPLE
                     HLALSGYLDLRACEDSERPTRLAEQVRAAADHAFDLSRDPMLRATLIALGDEEHVLILT
                     LHHIAADGWSMDVLVEEFGALYSAHSQGQPASLPALPVQYADYALWQQAQVKTLADGLD
                     YWSQQLAAAPEL"
     aSDomain        4..1089
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLU84_RS25345_AMP-binding.1"
                     /evalue="3.10E-78"
                     /label="BLU84_RS25345_AMP-binding.1"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="363"
                     /protein_start="1"
                     /score="255.1"
                     /specificity="substrate consensus: Asn"
                     /tool="antismash"
                     /translation="VVGLHVGRSLEQVVGVLAILKAGGAYLPLEPEHPNARLAYMCSDS
                     GVRTILSVGELAERCATWGEWTLSLDDEATLANYPSNTPEVAGLTPQHLAYVIYTSGST
                     GQPKGVLVPHGGVVNYLDHAQTYLQPHHRGAVMGTPLSFDATVTTLFAPLLAGKRLIVL
                     PSEAGALFAGLSHYLFDDGHDWLFKLTPAHLDGLYANYPAHSGPSARRHCLVIGGEQLT
                     TATVAKWQRDRLPNAEYVNEYGPTETVVGCSIYTVRTPADLARCGHAVAIGRPIQNTRL
                     YVLNELDEPVPAGAVGELCIGGDGVTRGYQNLDALTASRFREHRFGQGAVERLYHSGDL
                     VRCGHDGELEYLGRRDEQVKLR"
     aSModule        4..1626
                     /complete
                     /domains="nrpspksdomains_BLU84_RS25345_AMP-binding.1"
                     /domains="nrpspksdomains_BLU84_RS25345_PCP.1"
                     /locus_tags="BLU84_RS25345"
                     /monomer_pairings="Asn -> Asn"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       52..90
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU84_RS25345_0001"
                     /evalue="2.50E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="30"
                     /protein_start="17"
                     /score="17.5"
                     /tool="antismash"
                     /translation="LAILKAGGAYLPL"
     CDS_motif       286..345
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU84_RS25345_0002"
                     /evalue="9.00E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="115"
                     /protein_start="95"
                     /score="33.5"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGQPKGVLVPH"
     CDS_motif       682..705
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU84_RS25345_0003"
                     /evalue="1.60E+00"
                     /label="C4_LCL_164-176"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="235"
                     /protein_start="227"
                     /score="2.1"
                     /tool="antismash"
                     /translation="AKWQRDRL"
     CDS_motif       877..966
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU84_RS25345_0004"
                     /evalue="1.00E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="322"
                     /protein_start="292"
                     /score="46.6"
                     /tool="antismash"
                     /translation="PAGAVGELCIGGDGVTRGYQNLDALTASRF"
     CDS_motif       1054..1119
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU84_RS25345_0005"
                     /evalue="4.30E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="373"
                     /protein_start="351"
                     /score="37.9"
                     /tool="antismash"
                     /translation="YLGRRDEQVKLRGYRIELGEIE"
     aSDomain        1417..1626
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLU84_RS25345_PCP.1"
                     /evalue="9.00E-25"
                     /label="BLU84_RS25345_PCP.1"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="542"
                     /protein_start="472"
                     /score="78.7"
                     /tool="antismash"
                     /translation="ERVLATLWQELLERSEPVGAEDNFFRLGGHSLLATRMIVQINQRY
                     GLDLSVKDAFERQSLAALAEWVGQR"
     aSDomain        1681..2292
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLU84_RS25345_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.40E-67"
                     /label="BLU84_RS25345_Condensation_LCL.1"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="764"
                     /protein_start="560"
                     /score="219.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIAADG"
                     /tool="antismash"
                     /translation="PAPLSYAQQRLWFIDRLSGNSRQYNIAMPIAIQGRLDADALTAAL
                     RTIVQRHEVLRTVYREDTTGTVLQHALPLEHLALSGYLDLRACEDSERPTRLAEQVRAA
                     ADHAFDLSRDPMLRATLIALGDEEHVLILTLHHIAADGWSMDVLVEEFGALYSAHSQGQ
                     PASLPALPVQYADYALWQQAQVKTLADGLDYWSQQLAAAPE"
     CDS_motif       1693..1722
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU84_RS25345_0006"
                     /evalue="2.00E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="574"
                     /protein_start="564"
                     /score="10.7"
                     /tool="antismash"
                     /translation="SYAQQRLWFI"
     CDS_motif       1747..1860
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU84_RS25345_0007"
                     /evalue="1.10E-16"
                     /label="C2_LCL_024-062"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="620"
                     /protein_start="582"
                     /score="53.1"
                     /tool="antismash"
                     /translation="QYNIAMPIAIQGRLDADALTAALRTIVQRHEVLRTVYR"
     CDS_motif       2083..2148
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU84_RS25345_0008"
                     /evalue="6.00E-12"
                     /label="C3_LCL_132-143"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="716"
                     /protein_start="694"
                     /score="37.9"
                     /tool="antismash"
                     /translation="LHHIAADGWSMDVLVEEFGALY"
     CDS_motif       2197..2229
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU84_RS25345_0009"
                     /evalue="2.80E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="BLU84_RS25345"
                     /protein_end="743"
                     /protein_start="732"
                     /score="13.6"
                     /tool="antismash"
                     /translation="QYADYALWQQA"
ORIGIN
        1 agtgtggtcg gcctgcacgt ggggcgctcg ctggagcagg tggtgggtgt gctggcgatc
       61 ctgaaggccg gtggcgcgta tctgccgctg gagccggagc atcccaacgc gcgtctggcc
      121 tacatgtgta gcgacagcgg cgtacgcacg atcctgagcg tcggcgaact tgccgagcgc
      181 tgcgctacgt ggggtgagtg gacgctgagt ctggacgatg aagccacgct ggcgaactac
      241 ccgtcgaaca cgccagaagt agcgggcctg acgccgcaac acttggctta cgtgatctac
      301 acctcaggct cgaccggcca acccaagggc gtactggtcc cgcacggcgg cgtcgtgaac
      361 tacctcgatc acgcccagac ctacctgcaa ccgcaccacc gcggcgccgt catgggcacc
      421 ccgctgagct tcgacgccac cgtcaccacg ctgttcgccc cgctgctggc gggcaagcgt
      481 ctgatcgtgc tgccgtccga agccggcgca ttgttcgccg gcctgagcca ctacctgttc
      541 gacgacggac acgactggct gttcaagctg accccggcgc atctcgatgg cttgtatgcg
      601 aactacccgg cgcacagcgg tccgagcgca cgccgccact gcctggtgat cggcggcgaa
      661 cagctgacca cggcgaccgt cgccaagtgg cagcgcgacc gcttgcccaa cgccgagtac
      721 gtcaacgaat acggcccgac cgaaacggtc gtcggctgct cgatctacac cgtccgcaca
      781 cccgccgatc tggcgcgctg cggccatgca gtcgccatcg gccgcccgat ccagaacacc
      841 cgcctgtatg tgctcaacga actggacgaa cccgtgcccg cgggtgccgt cggcgagctg
      901 tgcatcggcg gcgacggcgt cacccgtggc tatcagaact tggacgcgct gaccgcaagc
      961 cgtttccgcg aacaccgctt cggccagggc gcagtcgagc gcctgtacca cagcggcgac
     1021 ctggtgcgct gcggccacga cggcgagctg gagtacctag gccgtcgcga cgaacaggtc
     1081 aagctgcgtg gctaccgcat cgaactgggc gagatcgaat cccagctcaa gcgcgatgcg
     1141 cgggtcagcg acgcggcagc ggcgatccag ggcgagggca acgaccgccg actggtcgcg
     1201 ttcgtcgcca gcaacgaaga agaaactgcc cacgccgcgc tggtcgacga actgcaacaa
     1261 gcgctgcgcc agagcctggc cgagttcatg cgcccaagtg cgatcgccgt gttggcgagc
     1321 ctgccgttga ccgcaaatgg aaaagtcgac cgcgcagcgc tgccgtcgct gggcggcgca
     1381 gtcgccgcgg cctaccgagc gccggagacc gaaaccgaac gcgtgctggc gacgttgtgg
     1441 caggaactgc tggaacgcag cgaaccggtc ggcgccgaag acaacttctt ccgtctcggc
     1501 ggccactcgc tgctggccac gcgcatgatc gtgcagatca atcagcgcta cggcctggat
     1561 ctgagcgtca aggatgcgtt cgagcgtcag tccctggctg cgttggccga gtgggtcggc
     1621 cagcgccgca gcgatgcgcg gcctgtggct ggggcgatcg tcgcacgtac cgaagcgggg
     1681 ccggcgccgc tgtcttacgc gcagcagcgc ctgtggttca tcgaccggct ctcgggcaat
     1741 agccggcagt acaacatcgc catgcccatc gcgatccagg gccgcctgga cgcggatgcg
     1801 ctgaccgcgg cgctgcgcac catcgtgcag cgccacgaag tgttgcgcac ggtctatcgc
     1861 gaggacacca ccggaaccgt gctgcagcat gcgctgccgc tggaacacct ggccctgagc
     1921 ggctatctgg atttgcgcgc ctgcgaagac agcgagcggc cgactcggct cgccgagcag
     1981 gtgcgggccg cggcggatca cgccttcgac ctgagtcgcg atccgatgct gcgcgcgacg
     2041 ctgatcgcgc tgggcgacga agagcacgtg ctgatcctga ccctgcacca catcgccgcc
     2101 gatggctggt cgatggacgt gctggtcgaa gagttcggtg cgctgtactc ggctcacagc
     2161 cagggtcagc ccgcatcgct gccggcgttg ccggtgcagt acgccgacta cgcgctgtgg
     2221 caacaggcgc aggtcaagac gctggccgat ggcctcgact attggtcgca acaactggcc
     2281 gcggcgccgg aact
//
