LOCUS       NZ_FNOG01000005        11275 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Lysobacter enzymogenes strain ATCC 29487, whole genome shotgun
            sequence.
ACCESSION   NZ_FNOG01000005
VERSION     NZ_FNOG01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lysobacter enzymogenes
  ORGANISM  Lysobacter enzymogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Lysobacter.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900106525.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 20:30:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,091
            Genes (coding)                    :: 5,036
            CDSs (with protein)               :: 5,036
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 8 of 55
            Pseudo Genes (incomplete)         :: 49 of 55
            Pseudo Genes (internal stop)      :: 4 of 55
            Pseudo Genes (multiple problems)  :: 6 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:36:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 427289
            Orig. end                         :: 438564
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNOG01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11275
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11275
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11275
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6275](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6275
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(19..1521)
                     /locus_tag="BLU84_RS14770"
                     /old_locus_tag="SAMN05421681_105220"
     CDS             complement(19..1521)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042736.2"
                     /locus_tag="BLU84_RS14770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_105220"
                     /product="GH-E family nuclease"
                     /protein_id="WP_074868571.1"
                     /transl_table=11
                     /translation="MGIEQQRLSGGGYVNLATWDDLRPTAHGPELQARAWNPNASRTGL
                     AFYDSTVHYMRNDWAREHMDVVPVRLGESDVLLFRNRESDTWHTAEALDIDHVQPWKQH
                     LTDLGARSQADAHIGYNDVGNLRALPSAINRGRTAAERALEQGVGSDAWRDWSDRHFRF
                     DPDAEHAPFDPVRDAARRTTTRFQDWALEDGRSALSFDTRVEGKWFEHQLSQQYRGTVS
                     MDADPDYHREIPLFECPVTRQLVTRDAFDIDHIRPFEDVARATLEARGGVITKAEALDL
                     YNDTSNLRLVSRAANCSHEWELDIHGEYLDRGDREHEEPENELDRAFVEIGPESLEERQ
                     AMDDLHVWLDRHPQPQPVQDDDEPVQVLQPRYALGNPRNPDTPLYNAAYAEVAGHNRQH
                     GLGLSADETANVAAALTLAVRNEGWAETSGVAYNPQQRAFYAYTGQPDAWVYTEVGLQQ
                     GRAQPANDSGRELHQLRHDPQQAQPGAQAMDVQQRDGRPMAM"
     gene            1780..2976
                     /locus_tag="BLU84_RS14775"
                     /old_locus_tag="SAMN05421681_105221"
     CDS             1780..2976
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014509586.1"
                     /locus_tag="BLU84_RS14775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_105221"
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="WP_083382613.1"
                     /transl_table=11
                     /translation="MPSLRDLSLSAVVAGFVAVLVGFTSSAAIVFSAARASGADQAEIA
                     SWMWALGLGMGATCIGLSLRYKVPVVTAWSTPGAAMLITAAAGLPLAQAIGAFVISAAL
                     TVALGFSGWLERALARLPASLAAGMLAGVLLRFGLDVFVSMQSHFALVFAMLLSFLLAR
                     RWLPRYAVLVALAVGVGVAGAQGGLHLEKLELAVAMPVWTTPQFSLAATVGVAIPLFVV
                     TMASQNVPGIAILRAHGYREVPASPLVGWTGVATLLLAPFGAFSLSLAAITAAICMGED
                     AHEDPRKRYMAAVAAGAFYLLTGVFGATVAAAFAAFPQALVMAIAGIALLATIGNGLAS
                     ALHDPQEREPALITFLVTASGFTVFQVGSVFWGMVAGVLATLVLRWRKPKPAATDPAR"
     misc_feature    1804..1806
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3136..3450
                     /locus_tag="BLU84_RS14780"
                     /old_locus_tag="SAMN05421681_105222"
     CDS             3136..3450
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLU84_RS14780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05421681_105222"
                     /product="hypothetical protein"
                     /protein_id="WP_074868576.1"
                     /transl_table=11
                     /translation="MEARRESALDSPGLRALLSPSVIVSLALALTAAALALLYFLRGGA
                     DLPAPVASATAAAAVSAPAAPAGATAATPPPAPEPVEFRCVNGLRVRKQGSSYSSDGRC
                     "
     gene            3541..4557
                     /locus_tag="BLU84_RS14785"
                     /old_locus_tag="SAMN05421681_105223"
     CDS             3541..4557
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /locus_tag="BLU84_RS14785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_105223"
                     /product="transporter"
                     /protein_id="WP_074868579.1"
                     /transl_table=11
                     /translation="MRIADASYGSNEDGLIWGYRFVPGQPAQPISTDAVAAFLSAPADG
                     DGGGFLWLHFSLSNTGSERYLRRALLMPDAFYDSLRSEVGSTRLELDDNALIAVVHDVL
                     FDSSFDASEVGTTTLCIGPRLLVSARLRPLRSVDQLRAAVRSGQLFRSPVELLAHLLRD
                     QASVLGEILRKCTQQVDRIEDRLLDNRIASDRTQLGSLRRSLVRLQRLLAPEPTALFRL
                     LSRPPEWIGRDDIGELQQAAEEFSTAIGDSVALVERVKLIQEELAALVNEQTGRTLFVL
                     TVVTVVALPINLVAGLFGMNVGGMPLAENPHGFWAVVLGLCVLTGVLAYAAFGRRRD"
     misc_feature    4617..4631
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.75"
                     /tool="antismash"
     misc_feature    4619..4634
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.35"
                     /tool="antismash"
     gene            4742..4882
                     /locus_tag="BLU84_RS27220"
                     /old_locus_tag="SAMN05421681_105224"
     CDS             4742..4882
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLU84_RS27220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05421681_105224"
                     /product="hypothetical protein"
                     /protein_id="WP_170849174.1"
                     /transl_table=11
                     /translation="MKKAIDMPLIIKSGPRLIEPLSTLEAHDVQPEVTSDELDFLEAHA
                     K"
     misc_feature    complement(4751..4764)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.55"
                     /tool="antismash"
     gene            5001..6275
                     /locus_tag="BLU84_RS14790"
                     /old_locus_tag="SAMN05421681_105225"
     CDS             5001..6275
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="BLU84_RS14790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_105225"
                     /product="YcaO-like family protein"
                     /protein_id="WP_074868582.1"
                     /sec_met_domain="YcaO (E-value: 1.7e-45, bitscore: 150.6,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIPLERERSLEQAERALRGFLDAQGWRAELQRAGAPFAATICTLY
                     DEADGRQLGNGFGKGEAEPARVGALFEAVEHLFTGKAPADAELSLRSGADCLADPLLAA
                     LPFRAAFVQQPERKLACRTYRSFDPREPEATLPLFLSYPTYLDWRPPQDDFDYAAALRF
                     GSNNGTAIGSSLEEAAVHAIGELIERDAWSLFLAAHYLGDPAAHGYRLAPDDLPAPLAR
                     RLSDASAQLQREILLIDARSDLGVPCFIATAADLHDGELLHPCGYGASLYPEHAATRAI
                     TELVQTLELPRLQPRLSGYPQAVLDALSAYPKLRDCAVFAIDRARLRRGRWDYPAPAPM
                     PPAELLPQLVARLRARGIDLHYAINHEEPGCFCVVNVVSMALERFFLVCSGIVMAPGQR
                     GRELLAGRLPVPGSAAAREAEAVLA"
     gene            complement(6469..7599)
                     /locus_tag="BLU84_RS14795"
                     /old_locus_tag="SAMN05421681_105226"
     CDS             complement(6469..7599)
                     /EC_number="3.4.-.-"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /locus_tag="BLU84_RS14795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_105226"
                     /product="M23 family metallopeptidase"
                     /protein_id="WP_074868587.1"
                     /transl_table=11
                     /translation="MLRRLFASALVLAAFACGAASAAPADTLDLAVPQAPAPIAVEGHE
                     RLLYELHLTNFGAAPLRLDGLSVLDADSGAALAAWRGEALAARGRVIGAQNAALDGALQ
                     PGERAVLYLELDLPAAMPRALRHKVEFSVPGAEPGQVEGAQLVPRPAPTLQLAPPLRGG
                     PWAAIYAPQWPRGHRRVFYTLDGRARLPGRYAIDFVRVDAQGRTTRGDADIAANALGYG
                     EPVLAVADGTIAAARDGIAENERVSQQTRNDFDRAAGNYVSLALGDGRYAVYEHLRPGS
                     VKVKPGQRVRRGQPIGELGFTGDSTGPHLHFHVADAAVPLLGEGVGYGFDGFRLLGYYP
                     GAKGLGERRWQPRADGVAAERRNELPEASSVIEFPR"
     gene            8024..8371
                     /locus_tag="BLU84_RS14800"
                     /old_locus_tag="SAMN05421681_105227"
     CDS             8024..8371
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLU84_RS14800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05421681_105227"
                     /product="hypothetical protein"
                     /protein_id="WP_139175015.1"
                     /transl_table=11
                     /translation="MDRRLLRAPRRPFPLVFAALLATTLAACSSSVPERQVREDFATLQ
                     QTGAIPAKARLERIELHDGWSDGAEIEVYFCLPAAEGPTRGCEPRNAGLSYQKLGERWQ
                     LFSVDAEQLVQ"
     misc_feature    complement(8727..8741)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     misc_feature    complement(8737..8750)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     misc_feature    complement(9304..9319)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.24"
                     /tool="antismash"
     misc_feature    complement(9820..9834)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
ORIGIN
        1 cgcggccgcg cccaccgctt acatcgccat cggccgccca tcgcgctgct gcacgtccat
       61 cgcctgcgcg ccgggctgcg cctgctgcgg gtcgtgccgc aactggtgca attcccggcc
      121 gctgtcgttg gccggctgcg cgcgcccctg ctgcaggccc acctcggtgt acacccaggc
      181 gtcgggctgg ccggtgtagg cgtagaaggc gcgctgctgc gggttgtagg ccaccccgct
      241 ggtctcggcc caaccttcgt tgcgcaccgc cagggtcagc gcggcggcga cgttggcggt
      301 ttcgtccgcg ctcaatccca ggccgtgctg gcggttgtgg ccggcgacct cggcataagc
      361 ggcgttgtac agcggcgtgt ccgggttgcg cggattgccc agcgcatagc gcggttgcag
      421 cacctgcacc ggctcgtcgt cgtcctgcac cggctgcggt tgcgggtggc ggtcgagcca
      481 gacgtgcaga tcgtccatcg cctgacgctc ctccagcgat tccgggccga tctcgacgaa
      541 ggcgcggtcg agctcgttct ccggctcctc gtgttcgcgg tcgccgcggt cgaggtactc
      601 gccgtggatg tccagctccc actcgtgcga gcagttggcg gcgcggctga ccaggcgcag
      661 gttgctcgtg tcgttgtaca ggtccagcgc ctcggccttg gtgatgacgc cgccgcgcgc
      721 ttccagcgtc gcccgggcga cgtcttcgaa cggacggatg tggtcgatgt cgaaggcgtc
      781 gcgggtgacc agttgccggg tcaccgggca ctcgaacagc gggatctcac ggtggtagtc
      841 cgggtcggcg tccatcgaaa cggtgccgcg gtactgctgc gagagctggt gctcgaacca
      901 cttgccctcg acccgggtgt cgaagctcag cgccgagcgt ccgtcttcca aggcccagtc
      961 ctggaagcgc gtggtggtgc ggcgggcggc gtcgcggacc gggtcgaacg gcgcgtgctc
     1021 ggcatcggga tcgaaacgga agtgacggtc cgaccagtcc ctccacgcgt ccgagccgac
     1081 gccttgctcg agcgcgcgct cggccgcggt gcggccgcgg ttgatcgcgc tgggcagcgc
     1141 gcgcaggttg ccgacgtcgt tgtagccgat gtgcgcgtcg gcctggctgc gcgcgcccag
     1201 gtcggtcagg tgctgcttcc acggctgcac gtggtcgatg tccagcgcct cggcggtatg
     1261 ccaggtgtcg gactcgcggt tgcggaacag cagcacgtcg ctctcgccga gcctgaccgg
     1321 caccacgtcc atatgctcgc gggcccagtc gttgcgcatg tagtgcacgg tggaatcgta
     1381 gaacgccagg ccggtgcggc tggcgttggg gttccaggcg cgcgcctgca gttccgggcc
     1441 gtgcgcggtc gggcgcagat cgtcccaggt ggccaggttc acgtagccgc cgccgctcaa
     1501 gcgctgttgt tcgatgccca tgatccgtcc cgctcgctat gcaatgcctg caacaacgtt
     1561 caaaggcggg ggcacaggcc aaaccgaagg cgtcgggccg cgcgcgcagc tcgggcctac
     1621 ccgcctgcgg tcgcggctgc cgggtacgct cgacccgagc gtgcgtccgc gccgcagcgc
     1681 ccgcgcgcaa gccgccgcgc gccgaaaatc cctcgccgcg cacagccggc cgcacggccg
     1741 atgccgcatg atcggggttt cgccccgccc ctcgcccgca tgccctcctt gcgcgatctg
     1801 tccttatccg ccgtcgttgc cggcttcgtc gctgtcttgg tcggcttcac cagttcggcc
     1861 gcgatcgtgt tctccgcggc ccgcgccagc ggcgccgacc aggccgaaat cgcctcgtgg
     1921 atgtgggcgc tgggcctagg gatgggcgcg acctgcatcg gcctgtcgct gcgctacaag
     1981 gtgccggtgg tgacggcgtg gtcgacgccc ggcgcggcga tgctgatcac cgccgcggcc
     2041 ggcctgccgc tggcgcaggc gatcggcgcg ttcgtgatca gcgccgcgct gaccgtcgcg
     2101 ctgggcttca gcggctggct cgaacgcgcg ctggcgcggc taccggcgag cctggccgcg
     2161 ggcatgctcg ccggcgtgct gctgcgcttc ggcctggacg tgttcgtctc gatgcagagc
     2221 cacttcgccc tggtgttcgc gatgctgctg agcttcctgc tggcgcggcg ctggctgccg
     2281 cgctacgcgg tgctggtcgc gctggccgtc ggcgtcggcg tggccggcgc gcagggcggg
     2341 ctgcacctgg agaagctcga actggcggtg gcgatgccgg tgtggaccac gccgcagttc
     2401 tcgctggccg ccaccgtcgg cgtggcgatc ccgctgttcg tggtgacgat ggcctcgcag
     2461 aacgtgcccg gcatcgcgat cctgcgcgcg cacggctacc gcgaggtgcc ggcctcgccg
     2521 ctggtcggct ggaccggcgt ggcgaccttg ctgctggcgc cgttcggcgc gttctcgctg
     2581 agcctggcgg cgatcaccgc ggcgatctgc atgggcgagg acgcccacga agacccgcgc
     2641 aagcgctaca tggccgcggt cgccgccggc gcgttctacc tgctcaccgg cgtgttcggc
     2701 gccacggtgg cggccgcgtt cgcggcgttt ccgcaggcgc tggtgatggc catcgccggc
     2761 atcgccctgc tcgccaccat cggcaacggc ctggcctcgg cgctgcacga tccgcaggaa
     2821 cgcgagccgg cgctgatcac cttcctggtc accgcctcgg gcttcaccgt gttccaggtc
     2881 ggctcggtgt tctggggcat ggtcgccggc gtgctcgcca ccctcgtgct gcgctggcgc
     2941 aagccgaagc cggcggcgac cgatccggcg cgctgagcgc cgcgcgggcc ggcggcgccg
     3001 gtccgcggcc gacgccagcg cccacagcgc gcgcccgaaa gcggcccgac gcctgccatt
     3061 ccggggtccg tacggacgat gccgccgcgc ccgcaccggg agtatgctcg gccgtcatcc
     3121 ccacggaagc gccgcatgga agcccgccgc gaatcggccc tggactcgcc cggcctgcgc
     3181 gccctgctca gccccagcgt gatcgtcagc ctggccctgg cgctgaccgc cgcggcgttg
     3241 gcgctgctgt acttcctgcg cggcggcgcc gacctgcccg cgcccgtcgc cagcgcgacc
     3301 gcagcggcgg ccgtgtccgc cccggccgca cccgccggcg ccaccgccgc gacgccccca
     3361 ccggcgccgg agccggtcga gttccgctgc gtcaacggcc tgcgcgtacg caagcagggc
     3421 agcagctatt ccagcgacgg ccgctgctga gcgcgcggcc gccggcgccg ccatgacgcg
     3481 gtcgcgcctt tgagctagcg taggcgccct gttctccgct tcatccggat cgccgctccg
     3541 atgcgcatcg ccgacgccag ctacggttcc aacgaagacg gcctgatctg gggctaccgc
     3601 ttcgtccccg gccagccggc gcaaccgatc agcaccgacg cggtcgccgc cttcctgtcg
     3661 gcgcccgccg acggcgacgg cggcgggttc ctgtggctgc acttctcgct gtccaacacc
     3721 ggcagcgagc gctacctgcg ccgcgccctg ctgatgcccg acgcgttcta cgacagcctg
     3781 cgcagcgaag tcggctcgac ccggctcgag ctggacgaca acgccctgat cgcggtcgtc
     3841 cacgacgtgc tgttcgactc cagcttcgac gcctccgaag tcggcaccac caccttgtgc
     3901 atcggcccgc gcctgctggt cagcgcgcgc ctgcggccgc tgcgctcggt cgaccagctg
     3961 cgcgcggcgg tgcgctcggg ccagctgttc cgctcgccgg tggagctgct cgcgcatttg
     4021 ctgcgcgacc aggccagcgt gctcggcgag atcctgcgca agtgcaccca gcaggtcgac
     4081 cgcatcgagg accgcctgct cgacaaccgc atcgccagcg accgcaccca actgggctcg
     4141 ctgcgccgca gcctggtgcg cctgcagcgg ctgctggcgc ccgagccgac cgcgctgttc
     4201 cgcctgctca gccggccgcc cgagtggatc ggccgcgacg acatcggcga gctgcaacag
     4261 gccgccgagg agttctccac cgcgatcggc gattcggtgg cgctggtgga acgggtcaag
     4321 ctgatccagg aagaactggc ggcgctggtc aacgagcaga ccgggcggac cttgttcgtg
     4381 ctgaccgtgg tcaccgtggt ggccttgccg atcaacctgg tcgccggcct gttcggcatg
     4441 aacgtcggcg gcatgccgct ggccgagaac ccgcacgggt tctgggcggt ggtgctgggc
     4501 ctgtgcgtgc tgaccggggt gctggcctac gccgcgttcg gccggcgccg cgactgacat
     4561 cgcaaccaca gcgcggctga atgcgcgccg cgttcacgcg cttggtcgct gcatcacaaa
     4621 ttcaaacaat tcgccgcacg ccgccgggcg gctttgctat agaggaacgg cagccgccgg
     4681 gagggcgcag gcccgcgtcg gtacgctgcg ccatcgcacg actaaccata tgagggacta
     4741 tatgaagaag gcaatcgata tgccgctgat catcaagagc ggcccgcgtc tgatcgaacc
     4801 gctgtcgacc ctggaagccc atgacgtcca gccggaagtg acttccgacg aactggactt
     4861 cctggaagcg cacgccaagt aagggcgcca caagcgcgtc cggtgtctgc cggcgcggcg
     4921 acggcacgga gcccgcctcc gtgccgccgc atccgcggcg ccgcggcgcg ccgcatccag
     4981 cgcaagggag agcgctccgc atgattcctc tggaacgaga acgcagcctc gagcaggccg
     5041 aacgcgcgct gcgcggcttt ctcgatgcgc aaggctggcg cgccgaactg cagcgcgccg
     5101 gcgcgccgtt cgcggcgacc atctgcacgc tctacgacga ggccgacggg cgccagctcg
     5161 gcaacggctt cggcaagggc gaagccgagc cggcgcgggt cggcgcgctg ttcgaagcgg
     5221 tcgagcattt gttcaccggc aaggcgcccg ccgacgccga actgagcctg cgcagcggcg
     5281 ccgattgcct ggccgatccg ctgctggccg cgctgccgtt ccgcgccgcg ttcgtccaac
     5341 aacccgagcg caagctcgcc tgccgcacgt atcgctcctt cgacccgcgc gaaccggaag
     5401 cgacgctgcc gctgttcctg tcctacccca cctacctcga ctggcgtccg ccgcaggacg
     5461 acttcgatta cgccgccgcg ctgcgctttg gttccaacaa cggcaccgcg atcggcagca
     5521 gcctggaaga agccgcggtg cacgcgatcg gcgaattgat cgagcgcgat gcctggtcgc
     5581 tgttcctggc cgcgcattac ctcggcgacc cggccgccca cggctaccgc ctggcgccgg
     5641 acgacctgcc cgcgccgctg gcccggcgcc tgagcgacgc cagcgcgcaa ttgcagcgcg
     5701 agatcctgct gatcgacgcg cgcagcgacc tcggcgtgcc ctgcttcatc gccaccgccg
     5761 ccgacctgca cgacggcgag ctgctgcacc cgtgcggcta cggcgcctcg ctgtatcccg
     5821 aacacgccgc cacccgcgcg atcaccgaac tggtgcagac gctggaactg ccgcggctgc
     5881 agccgcggct gagcggctac ccgcaggcgg tgctcgacgc cttgtccgcg tatccgaagc
     5941 tgcgcgactg cgcggtgttc gcgatcgacc gcgcgcgcct gcgccgcggc cgctgggact
     6001 acccggcgcc ggcgccgatg ccgccggccg agttgctgcc gcaattggtc gcgcgtctgc
     6061 gcgcgcgcgg catcgacctg cattacgcga tcaaccacga agaacccgga tgcttctgcg
     6121 tggtcaacgt ggtgtcgatg gcgctggagc gcttcttcct ggtgtgctcg ggcatcgtca
     6181 tggcgccggg gcagcgcggg cgcgagctgc tggccggacg cttgccggtg cccggcagcg
     6241 cggcggcgcg cgaggcggag gctgttctgg cctgagttcg tcgccgctgt ccgcgctcgg
     6301 ttgggcgcgg cgcggtttcg cggtcgcgca ccgaattcaa tcgtccctgc gcaacaggct
     6361 cagaccactg cgatccgtag caggcgcttc agcgcgcggg cccgtttcag cgaagtggcg
     6421 tcctcgggtt ccgctactga cgcagatcgg atagatgcgg cagtccgctc aacgcggaaa
     6481 ttcgatcacc gaactggcct cgggcaattc gttgcggcgc tcggcagcga ccccgtcggc
     6541 gcgcggctgc caccgccgtt cgcccagccc tttcgcgcca gggtaatagc cgagcaagcg
     6601 gaagccgtca aagccgtagc ccactccttc gccgagcagc ggcaccgctg cgtcggcgac
     6661 gtggaaatgc aggtgcggcc cggtcgaatc gccggtgaag ccgagttcgc cgatcggctg
     6721 gccgcggcgc acgcgctggc ccggcttcac tttcacgctg cccggccgca ggtgctcgta
     6781 caccgcgtaa cggccgtcgc cgagcgccag cgacacgtag ttgccggcag cgcggtcgaa
     6841 atcgttgcgg gtctgctgcg acacccgctc gttctcggcg atgccgtcgc gcgccgcggc
     6901 gatggtgccg tcggccaccg ccagcaccgg ctcgccgtag cccagcgcgt tggcggcgat
     6961 gtcggcgtcg ccgcgggtgg tgcggccttg cgcgtcgacg cggacgaagt cgatggcgta
     7021 gcgcccgggc aggcgcgcgc ggccgtcgag ggtgtagaac acccgccgat gcccgcgcgg
     7081 ccactgcggc gcgtagatcg ccgcccacgg gccgccgcgc aacggcggcg ccagttgcag
     7141 cgtcggcgcc gggcgcggaa ccagttgcgc gccctcgacc tgccccggct cggcgccggg
     7201 cacggagaat tcgaccttgt gccgcagcgc gcgcggcatc gccgcaggca gatccagttc
     7261 caggtacagc accgcgcgct cgcccggctg cagcgcgccg tccagcgccg cattctgcgc
     7321 gccgatcacc cggccgcgcg cggccaaggc ctcgccgcgc caggccgcca gcgccgcgcc
     7381 gctgtcggcg tcgagcacgc tgagcccgtc caggcgcagc ggcgccgcgc cgaagttggt
     7441 gaggtgcagt tcgtacagca agcgctcgtg gccttcgacc gcgatcggcg cgggcgcttg
     7501 cggcacggcc agatcgagcg tgtccgcggg cgcggcggag gcggcgccgc aggcgaaggc
     7561 ggcgaggacg agagcggacg cgaacaggcg gcgcagcatg cggcgattcc gggccggagg
     7621 gatgggcgag cgggagcggt tcggcgaaga cgcgagccag gcccgcagct tcggccaagc
     7681 cggcggccgc gtcccctgcc gtttgtcatc aaccccgact gcaacgcacc ggtcgcgggg
     7741 cgagcacgaa cgtgcgaact ccgattcgga tcagcggctt cagcgtggtc caaagcgcga
     7801 agcagcgggg ttgcgattgc cttggcgttg cgaagcggcg acacgtacgc gtggatcgtc
     7861 gcctgcctgt ctgtcgttgc tgcggcgccg cggcggcggc gcccgtgaaa cgcgaacggg
     7921 tgtgggttcc ggccgtgggt tttggtgctt gcgttcgcat cgccagtgga gaatcatccc
     7981 acctcccgcg gccaaggagg ccgcctgccc gccccgcccc gacatggacc gtcgtcttct
     8041 gcgcgccccg cgccgcccgt tcccgcttgt gttcgccgcg ctgctggcga cgacgctggc
     8101 cgcctgctcc agctcggtgc ccgagcgcca ggtgcgcgaa gacttcgcca ccttgcagca
     8161 gaccggcgcg attccggcca aggcgcggct ggaacgcatc gagctccacg acggctggag
     8221 cgacggcgcc gaaatcgagg tgtatttctg cctgcccgcc gccgagggcc cgacccgcgg
     8281 ctgcgaacca cgcaatgccg ggttgtcgta ccagaagctc ggggaacgct ggcagttgtt
     8341 ttcggtggac gctgagcagt tggtgcagtg agcggggcgt ggcggcttga ggccttgcga
     8401 caagcatcgt gcgcgggcgc cgaacgctcc cgccgccgtc gccgcgagat cgcgcacgga
     8461 cgccgaactc tccaaccgtc atccccgcga acgcggggat ccagggcttt atccaggcat
     8521 gactctgaag tctctggatc cccgcgttcg cggggatgac ggcttgagag agacgcggcg
     8581 aaacctacct accgtcattc ccgcgaaagc gggctccggt ttgcttcggc ggagccgaat
     8641 atccagggct taatccaggc atgacgctga agtctctgga tccccgcctt cgcggggatg
     8701 acggcttgga gggacgcggg aaattcagcg acgcggttga tcgggacgaa tggcctaggc
     8761 gagtcaatca ccacgcctgc gacaaacgct acgcacgcag tacgacacaa cgttcaggcc
     8821 ggctgcgcct gctcgccgtc accctcgccg accccacccg accgcgccag ccacgcctcg
     8881 ttcttccccg gccgacgcag cttgtccagg tagtagtaca ccaccggcgt cgtcagcaag
     8941 gtcagcacct ggctggccat cagaccgccg atgatcgcca cgcccaacgg ctggcgcagt
     9001 tcggcgcctt cgccgaagcc gatcgccagc ggcagcgcgc ccagggcggc ggccagggtg
     9061 gtcatcagga tcggccggaa gcgcagcatc gaggcctcgt agatcgcctc gcgcgcgccg
     9121 atgccgcgcg agcgctcggc ctccagggcg aagtcgatga tcaagatcgc gttcttcttg
     9181 acgatgccga tcagcagcac cagcccgatc agcgcgatca ccgagaactc catgcgcagc
     9241 agcatcagcg ccagcaccgc gccgatcccg gccgagggca gcgtcgacag cacggtcagc
     9301 ggatggatca ggctttcgta gaggatgccc agcacgatgt agatcgccac gatcgccgcc
     9361 aggatcagca acggctggct cttggtcgtg tcctggaacg ccttggcggt tccctggaaa
     9421 ctgccgtgca cgttggccgg caggccgatc tgcgcctgcg cgcgggcgat ggcgtcctgg
     9481 gcgtcgctga gcgagcggtc cggcgcgagg ttgaacgaga tggtggtcgc cagctgcgcg
     9541 tcctgatggt tgatcgaggt cggcgcagcc gcgtcggcga agcgcgcgat cgccgccagc
     9601 ggcaccatct gccgcgggct ctgggtcagc gcgctgcctt gcgaagcgcc gcgcaccggc
     9661 gaggcggtcg ggcccgactg ggcctggcct acggccggcg tcgccttggc cggcacgtac
     9721 aggtcgttga gcgcggacgg gtcgcgtgcg tagaccgggt cgacctcctg gatcacgtgg
     9781 tactggttga ggttcttgta gatggtcgac acctggcgct ggccgaacgc atcgtagagc
     9841 gcgttgtcga tatcgaccgg ggcgatgccc aggcgcgagg cgctggagcg gtcgatggtg
     9901 acgtagcttt ccaccccgtg cggctgggtg tcggaatcca cgtcggtcag ttccggctgc
     9961 tgcttcatcg ccgcggtcag ctggtcggcc cagcgcctta ggtcgtcgct gctgtcgctc
    10021 ttgagcgtgt actgatacgt cgaattgctc tgccgcccgc ccaggcgcac gtcctgcacc
    10081 gggttgagga acaaggtcgc gccgctgatg cgcatcagct tgggccgcag ccgcgcgatc
    10141 acctgctcgc tgctgtcgcg gcgctgcggc ttgggcttga gggtcacgaa cacgaagccg
    10201 ccgccggcgc gcgagccgcc ggtaaagccg accacggtgg ccaccgccgg atcgtggcgc
    10261 accacctcga cgatctcctg cagcttgccc tgcatggcct ggaacgaaat gctctggtcc
    10321 gcgcgcaggc cgccggtcat ctggccggtg tcctgttcgg ggaagaagcc cttgggaatc
    10381 ttgacgaaca accaggcgtt gagtgcgacg gtcagcgcca gcagggccag catcagcgcg
    10441 gcgtggtcga gcgcccagcg caggctggcg ccgtaggtct ggcgtagccg ctccaggccg
    10501 cgctccagcc acgcgccgat ccggcccggc ggccgttcct gcgaatgcgg gcgcagcagc
    10561 caggcgcaca tcatcggcgt ggtggtcagc gacaccagca gcgagatcag caccgccgcc
    10621 gacaaggtca ccgcgaactc gcggaacagc cggccgacga tgccgcccat gaacagcagc
    10681 gggatgaaca ccgccaccaa cgacaagctg atcgaaacca cggtgaaacc gacttcgcgc
    10741 gcgcccagca gcgcggcctg gaagcgctcc atgccgcgtt ccagatggcg gctggtgttc
    10801 tccaccacca cgatcgcgtc gtcgacgacg aagccggtgg cgatggtcag cgccatcagg
    10861 ctgaggttgt tgaggctgaa gccgagcagg tacatcaccg cgaccgcgcc gaggatcgac
    10921 acggtgaccg ccacgcccgg caccaaggtc gcgcgccagc tccgcaggaa cacgctgacc
    10981 accaggatca ccagcaccag ggcgatggcc agggtggcgc cgacttcgtc gagcgaggcg
    11041 cggatggtgt tggtgcggtc ggaggcgatc gacagcttga tgtcggccgg caactgcttg
    11101 gccagggtcg gcagcgccgc gcgcacaccg tcgacggtgc ggatgatgtt ggcgccgggc
    11161 tggcggctga tcaccgccac caccgccggc tggccgttga acaggcccat cgtgtgcacg
    11221 tcctcgacgc tgtcgacgac ccgcgccaca tcgtgcaggc gcaccgcggc gttgt
//
