LOCUS       NZ_FNOG01000003        10885 bp    DNA     linear   CON 05-MAY-2024
DEFINITION  Lysobacter enzymogenes strain ATCC 29487, whole genome shotgun
            sequence.
ACCESSION   NZ_FNOG01000003
VERSION     NZ_FNOG01000003.1
KEYWORDS    WGS; RefSeq.
SOURCE      Lysobacter enzymogenes
  ORGANISM  Lysobacter enzymogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Lysobacterales;
            Lysobacteraceae; Lysobacter.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900106525.1-RS_2024_05_05
            Annotation Date                   :: 05/05/2024 20:30:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,091
            Genes (coding)                    :: 5,036
            CDSs (with protein)               :: 5,036
            Genes (RNA)                       :: 62
            rRNAs                             :: 3, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 8 of 55
            Pseudo Genes (incomplete)         :: 49 of 55
            Pseudo Genes (internal stop)      :: 4 of 55
            Pseudo Genes (multiple problems)  :: 6 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 15:36:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 638468
            Orig. end                         :: 649353
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNOG01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10885
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10885
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10885
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5885](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5885)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(198..213)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.84"
                     /tool="antismash"
     gene            693..1004
                     /gene="sugE"
                     /locus_tag="BLU84_RS10600"
                     /old_locus_tag="SAMN05421681_103580"
     CDS             693..1004
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="sugE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006451011.1"
                     /locus_tag="BLU84_RS10600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103580"
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="WP_074866221.1"
                     /transl_table=11
                     /translation="MPWVLLVFAGLFEIGWAIGLKYTEGFTRLWPSVGTVVSMAISVGL
                     LGLAMKTLPVGTAYAIWVGIGAVGTVILGMVLFNEPATALRIASIVLIVAGLVGLKLA"
     misc_feature    715..730
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.03"
                     /tool="antismash"
     misc_feature    complement(1069..1080)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 21.4"
                     /tool="antismash"
     gene            1097..1543
                     /locus_tag="BLU84_RS10605"
                     /old_locus_tag="SAMN05421681_103581"
     CDS             1097..1543
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484891.1"
                     /locus_tag="BLU84_RS10605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103581"
                     /product="acetyltransferase"
                     /protein_id="WP_074866225.1"
                     /transl_table=11
                     /translation="MPIRERQAADHPRLLELWERSVRATHDFLSDADIAELRPQVRDIY
                     FDAVEIWIYEDAAGLAGFIGLSGAQVEMLFLEPERRGQGIGTRLLDHARGLRGALTVDV
                     NEHNPQAHGFYRRYGFVDTGRSPLDGQGRPFPLIHMALPRDAAG"
     gene            complement(1625..2236)
                     /locus_tag="BLU84_RS10610"
                     /old_locus_tag="SAMN05421681_103582"
     CDS             complement(1625..2236)
                     /GO_function="GO:0004568 - chitinase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006032 - chitin catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012409.4"
                     /locus_tag="BLU84_RS10610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103582"
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="WP_074866228.1"
                     /transl_table=11
                     /translation="MFAFDDDQFLDGYRKRFGPLREDLAKALRFLVGRIEQDSSFSLNP
                     VHRYQIAYCLATFKWETAHTLRPIDEFGDDARFERLYGPDTRIGKALGNTRRGDGARFH
                     GRGYVQLTGRNNYDRAGRFLGLDLVNKPEDAKKPEVAYAIAMQGMKQGWFTGKKLDNYF
                     KPGQLPNYEEARRIINGQDKAQTIADIARRFDELLVNALE"
     misc_feature    2220..2235
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 23.79"
                     /tool="antismash"
     gene            complement(2371..3399)
                     /locus_tag="BLU84_RS10615"
                     /old_locus_tag="SAMN05421681_103583"
     CDS             complement(2371..3399)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLU84_RS10615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05421681_103583"
                     /product="hypothetical protein"
                     /protein_id="WP_074866231.1"
                     /transl_table=11
                     /translation="MTDTATSPAYFWTPGAGPDPQALRRLRALTRLPKRAMGEAWFMGE
                     ERARFVSLMHDDPRQWPALDLSNALFELTSGTVNFGPRREWQLWCAFLLPRALELLDPG
                     SGSYVHHLHPALVTAALMNLERPALAYLPPNVPRDLLDTLGRAMFVPSIWQGTRMRADA
                     WCLPFSAGTIHGDMIDPAGAFSASCTLALKLLEPAQLSGWLASALAIDDPYWRAAWVTW
                     LHAAAPMILDGAYPDELDADEPSEWRPDTLDEPNPAVWENSHTLKREDREHAAGQRAQG
                     FIDDARRDAFLAALRGLLGRGRLEQWALALSAEAAYPGQLDYARRQYAAAARSLPERYR
                     LD"
     gene            complement(3429..4079)
                     /gene="rnt"
                     /locus_tag="BLU84_RS10620"
                     /old_locus_tag="SAMN05421681_103584"
     CDS             complement(3429..4079)
                     /GO_function="GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /GO_process="GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rnt"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467237.1"
                     /locus_tag="BLU84_RS10620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103584"
                     /product="ribonuclease T"
                     /protein_id="WP_074866235.1"
                     /transl_table=11
                     /translation="MIDPGPAPSAASAAPVPATLATRFRGYLPVVVDVETGGFDWNKHA
                     LLEIAVQPIELDADGLLVPGEVASSHVVPAAGTLIDPKSLEVTGIDIDHPFRDAKPERQ
                     ALEAVFAPVREAVKRHGCQRAILVGHNAHFDLNFLNAAVARSGHKRNPFHPFSVFDTVT
                     LAGVAYGQTVLARAVQAAGFVWNGEEAHSAVYDTNRTAELFCKIVNAWPARGA"
     gene            complement(4244..4996)
                     /locus_tag="BLU84_RS10625"
                     /old_locus_tag="SAMN05421681_103585"
     CDS             complement(4244..4996)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005412767.1"
                     /locus_tag="BLU84_RS10625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103585"
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="WP_074866238.1"
                     /transl_table=11
                     /translation="MNDASSRLRAQQFELTRHLRDPSASPAPAGIEDRRLSIYRDLLFN
                     NIASLLSGNFPVIESILGEERWERLVRDFYRDHRCHTPLFAEIAREFQRYLQNLDALDP
                     PFLLELAHYEWVELALQLSEASAARDDAGIGDAGLLEHVPRLSPLAWPLAYAWPVHRLG
                     PDFQPAQPPPTPTLLLLRREADGEVRFSELSPLAYRLLQRLEESPQLIAREQLRALAAE
                     AGSDDEATFLDMGAALLRQMRATGVVYF"
     gene            complement(5001..5885)
                     /locus_tag="BLU84_RS10630"
                     /old_locus_tag="SAMN05421681_103586"
     CDS             complement(5001..5885)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534610.1"
                     /locus_tag="BLU84_RS10630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103586"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_074866241.1"
                     /sec_met_domain="DUF692 (E-value: 1.4e-111, bitscore:
                     367.3, seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDRTHSLTPDAVGLGLRRALLGPLQSAPHAPGAARDFDFLELAPE
                     NWIGVGGRYGEQLRELSARYPLVCHGLSLSLGGTAALDETFLGRVRRFLDEHAVACYSE
                     HLSYCSDDGHLYDLLPIPFTEEAVHHVAARIRRTQDIVGRRIAVENASYYAAPHQEMSE
                     IEFTRAVLAEADCDLLLDVNNVYVNSINHRYDALEFIRAMPHERIAYLHVAGHYDEADD
                     LKVDTHGAPVKREVWDLLADAYRLFGPRPTLLERDFNFPPYAELVDELGLVRRILRESA
                     APAARDSARRAHA"
     misc_feature    complement(5845..5859)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.42"
                     /tool="antismash"
     gene            complement(5899..6300)
                     /locus_tag="BLU84_RS10635"
                     /old_locus_tag="SAMN05421681_103587"
     CDS             complement(5899..6300)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646537.1"
                     /locus_tag="BLU84_RS10635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103587"
                     /product="hypothetical protein"
                     /protein_id="WP_074866245.1"
                     /transl_table=11
                     /translation="MSKQINKPIALALSATLLGGLSLSSSAFAMSDLSQGYLLGAQQAQ
                     TAEKAADAKATDAAKKAEGSCGADKKKAEGKCGADKKKHAEGKCGEGKCGADKKTGEGK
                     CGADKKGAAPAAKKAGEGKCGEGKCGGSM"
     gene            complement(6516..7031)
                     /locus_tag="BLU84_RS10640"
                     /old_locus_tag="SAMN05421681_103588"
     CDS             complement(6516..7031)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /locus_tag="BLU84_RS10640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103588"
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="WP_074866248.1"
                     /transl_table=11
                     /translation="MTPLLANGDLAAARAGDTAALERVLRHSRQDLRRYAEFHCVINDI
                     EDAVQESLIAVSRKLRDLRVLECFVSWTFRIVKRECNRLKRGRRLLSGQPITDEIAPVV
                     TPEPAEWRHDVAVALESLPAHYRQIILLRDLEGLTVAEIGEELSLSREAVKSRLHRARV
                     LAREYLSP"
     misc_feature    complement(7020..7022)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(7294..7309)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 19.52"
                     /tool="antismash"
     gene            complement(7411..8121)
                     /gene="phoU"
                     /locus_tag="BLU84_RS10645"
                     /old_locus_tag="SAMN05421681_103589"
     CDS             complement(7411..8121)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="phoU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005994733.1"
                     /locus_tag="BLU84_RS10645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103589"
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="WP_074866251.1"
                     /transl_table=11
                     /translation="MSNQMHDHIVKSYDDEQRRLLDETLRMGEIAAAQLESALDVVQRR
                     DDKAAERIIANDEAIDALEQEISHDVMKLALRGPMARDLREILAAIRIASDIERVGDYA
                     ANVAKRSTALNLSPPLPHVAGLHALGTLAVKQLRDVLVAYRDNDIELAQRVRDRDAEVD
                     TAYTGLFRELLTYMMEDARSITACTHLLFMAKNIERVGDHATNIAENVWFLVKGDEPLP
                     PREKRDTTNTTASV"
     misc_feature    8090..8105
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.64"
                     /tool="antismash"
     gene            complement(8258..9082)
                     /gene="pstB"
                     /locus_tag="BLU84_RS10650"
                     /old_locus_tag="SAMN05421681_103590"
     CDS             complement(8258..9082)
                     /EC_number="7.3.2.1"
                     /GO_component="GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstB"
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534613.1"
                     /locus_tag="BLU84_RS10650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103590"
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="WP_074866255.1"
                     /transl_table=11
                     /translation="MNDARSSDARLSVATPGREAVGANPVKLAARDLNFYYDKFHALKS
                     INLEIPEKQVTALIGPSGCGKSTLLRIFNRIYALYPKLEARGQVLLDGDNILDPKYPMN
                     RLRSKVGMVFQKPVPFPMTIFENVAYGIRHHEKLSKSEMNDRVEHALRQGALWDEVKDK
                     LGQSALGLSGGQQQRLCIARAVALKPSVLLLDEPTSALDPISTSRIEQLVEELKKDYTI
                     AIVTHNMQQAARVSDFTAFMYLGDLIEHGPTEQIFSKPTKQQTEDYITGRFG"
     gene            complement(9120..10025)
                     /gene="pstA"
                     /locus_tag="BLU84_RS10655"
                     /old_locus_tag="SAMN05421681_103591"
     CDS             complement(9120..10025)
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene="pstA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010343977.1"
                     /locus_tag="BLU84_RS10655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421681_103591"
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="WP_074866258.1"
                     /transl_table=11
                     /translation="MSAVASKHDAAEAARHRTADSLYRRRRVVNAVSVLLACGAALFGL
                     FFLGWILYTLISKGIGGINLALFTQNTPPPMQEGGLLNAFFGSAVMCLIAIVIGTPLGI
                     AAGTWLAEYGAGRKMGTVVRFVNDILLSAPSIVLGLFVYTLVVMQTGGNFSALAGAIAL
                     AFIVLPVVVRTTDEMLRLVPPQMREAALSLGVPQWKVIMQVLYRSASAGIVTGVLLALA
                     RISGETAPLLFTAFGNQYWSHNVLQPMASVPVVMNQFAGSPYETWQTLAWAGALVLTFF
                     VLVVSLLARALVLRNRISND"
     misc_feature    10038..10054
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.72"
                     /tool="antismash"
ORIGIN
        1 cgccgccgac agcgtgccgg tggtcccggt gaccaacctg gcgcgcagcc tgcgtgagtt
       61 gcagcggctg ggcgtgtgga tctacggcct ggccggcgag gcgcaggcgt cgctgtattc
      121 gctggacctg cgcggcaacg tcgcgctggc gctgggcggc gaagccgacg gcctgcgccg
      181 gctgacccgc gagacctgcg accagctggt caagatcccg atgcccggcg gcgacgcggc
      241 cacgggcgtg gaaagcctga acgtgtcggt ggcggcgggc gtgacgttgt tcgaagcggt
      301 gcgtcagcgc ggttgagggc agggcgcggc gatctggccg gaagcgtcgg gactgaagtc
      361 cctcccacaa caacccttct cgggccgcgc aggctgttgc gagaggactt ttgtggcggt
      421 tttttgccgg agggcttttg tgggagggac ttcaatcccg atgctttccg ctccgatcca
      481 gcgatccagc gatcccgcac cgccgtaaac gccgcgaaga cttatgtaac cccccgctga
      541 ccccacagcc cgctcctcgg cctcatcgcc gaacgcctcg atataattcg ccgcgaccga
      601 cctgggacgg cccaggtccg cgcagccgga tccactcctg tgcggcgcgc gttacatccg
      661 gggacggccc catcgatcat gggagctgca tcatgccttg ggttcttctc gtcttcgccg
      721 gcctgttcga aatcggctgg gccatcggcc tcaaatacac cgagggtttc acccgcctgt
      781 ggccgtcggt cggcaccgtc gtgtcgatgg cgatcagcgt cggcctgctc ggcctggcga
      841 tgaaaacctt gccggtcggc accgcctacg cgatctgggt cggcatcggc gcggtcggta
      901 cggtcatcct cggcatggtg ctgttcaacg agccggccac cgcgctgcgc atcgccagca
      961 tcgtgctgat cgtcgccggc ctggtcggct tgaaactggc ctgaggcgct ggcggccacg
     1021 tccgcgtccc ggcatcatcg tcgcgcgcgg cgccgcactt gcggcgccgc gttcgtttcc
     1081 acggaaccca accgccatgc ccatccgcga acgccaggcc gccgaccacc cgcggctgct
     1141 cgaactgtgg gagcgctcgg tgcgcgccac ccacgatttc ctcagcgacg ccgacatcgc
     1201 cgagctgcgc ccgcaagtgc gcgacatcta tttcgacgcg gtcgagatct ggatctacga
     1261 ggacgccgcc ggcctggccg gtttcatcgg cctgtccggc gcgcaagtgg agatgctgtt
     1321 cctcgaaccc gagcggcgcg ggcagggcat cggcacgcgc ctgctcgacc acgcgcgcgg
     1381 cctgcgcggc gcgctcaccg tcgacgtcaa cgagcacaac ccgcaggcgc acggtttcta
     1441 ccggcgctac ggcttcgtcg acaccggacg ctcgccgctg gacgggcagg gccggccgtt
     1501 tccgctgatc catatggcgc tgccgcgcga cgcggcgggc tgagcgccgg cgcgcgccgc
     1561 ttcggcgcac gcatcattcc aacgcaggcg tcgctgaagc gcacgcttcg cgtcaaaaca
     1621 cgcatcactc caacgcgttc accaacaact cgtcgaagcg ccgcgcgata tcggcgatgg
     1681 tctgcgcctt gtcctggccg ttgatgatcc gccgcgcttc ttcgtaattc ggcaactggc
     1741 ccggcttgaa gtaattgtcg agtttcttgc cggtgaacca gccctgcttc atgccctgca
     1801 tggcgatggc ataggcgact tccggcttct tcgcgtcttc gggcttgttc accagatcca
     1861 gaccgaggaa gcggccggcg cggtcgtagt tgttgcggcc ggtgagctgc acataaccgc
     1921 ggccgtggaa gcgcgcgccg tcgccgcgcc gggtgttgcc cagcgccttg ccgatgcggg
     1981 tgtcggggcc gtacaggcgc tcgaagcgcg cgtcgtcgcc gaactcgtcg atcggccgca
     2041 gcgtgtgcgc ggtttcccac ttgaacgtcg ccaggcaata ggcgatctgg tagcggtgca
     2101 ccggattgag gctgaaactg gaatcctgct cgatccggcc gaccaggaag cgcagggcct
     2161 tggcgagatc ctcgcgcagc gggccgaagc gcttgcggta gccgtcgaga aactggtcgt
     2221 cgtcgaaagc gaacatgctt tgctccccat ccgtggatcg cggcgtgggc gccgcgcgcg
     2281 gcggagggcc gcgcattcca tcgaacgcgg gcgccggtaa cgggcggcca aggcggcaat
     2341 gttgcgcgac cgcaacatcc cgcggcggcc tcagtcgagc cggtagcgct ccggcaggct
     2401 gcgcgccgcg gcggcgtact ggcggcgcgc gtagtccagt tgccctggat aagccgcctc
     2461 ggcggacagg gcgagcgccc actgttccag ccggccgcgg ccgagcaggc cgcgcagcgc
     2521 ggcgaggaag gcgtcgcgac gcgcgtcgtc gatgaagccc tgcgcgcgct ggccggctgc
     2581 gtgctcgcga tcctcgcgct tgagcgtatg gctgttttcc cagaccgccg ggttcggttc
     2641 gtccaaggta tccggccgcc attcgctcgg ttcgtccgca tccagttcgt ccggatacgc
     2701 cccgtccagg atcatcggcg cggccgcatg cagccaggtc acccacgccg cgcgccaata
     2761 cggatcgtcg atggcgagcg ccgaggccaa ccatcccgac agttgcgccg gttccagcaa
     2821 tttcagcgcc agcgtgcagg aggcggagaa ggcgccggcg ggatcgatca tgtcgccgtg
     2881 gatcgtgccg gcgctgaacg gcaggcacca ggcgtctgcg cgcatgcgcg tgccttgcca
     2941 aatcgaaggc acgaacatcg cgcggcccag cgtgtcgagc aggtcgcgcg gcacgttcgg
     3001 cggcagatac gccaatgccg gtcgttctag attcatcagc gccgcggtga ccagcgccgg
     3061 atgcagatga tggacgtagc tgccggatcc agggtcaagc aattccagcg ctcgtggcaa
     3121 caagaacgcg caccacaatt gccattcgcg ccgcggcccg aaattgacgg tgccggaagt
     3181 tagttcgaac agcgcgttgc tcagatccag cgcaggccat tgacgtgggt catcgtgcat
     3241 cagcgacacg aagcgagcgc gttcctcgcc catgaaccaa gcctcgccca tcgcccgctt
     3301 gggcaggcgg gtcagcgctc gcaggcgccg cagcgcctgc gggtccgggc ctgcgcccgg
     3361 cgtccagaaa taggccggag aggtcgcggt gtcggtcatg actgcatcct tgcagccgcg
     3421 gcggcgaatc aggcgccgcg cgccggccag gcgttgacga tcttgcagaa cagctcggcc
     3481 gtgcggttgg tgtcgtacac cgccgaatgc gcttcctcgc cgttccagac gaagcctgcg
     3541 gcctgcaccg cgcgcgccag cacggtctgg ccgtaggcga cgccggccag ggtcacggtg
     3601 tcgaacacgc tgaagggatg gaacgggttg cgcttgtggc cgctgcgcgc gaccgcggcg
     3661 ttgaggaagt tgaggtcgaa gtgggcgttg tggccgacca ggatcgcgcg ctggcagccg
     3721 tggcgcttga ccgcttcgcg caccggcgcg aacaccgctt ccagcgcctg ccgctcgggc
     3781 ttggcgtcgc ggaaggggtg atcgatgtcg atgccggtga cttccagcga cttggggtcg
     3841 atcagggtgc cggcggcggg caccacgtgc gaactggcga cctcgccggg gaccaggagg
     3901 ccgtcggcgt cgagctcgat cggctgcacg gcgatttcga gcagggcgtg cttgttccag
     3961 tcgaagccgc cggtttccac gtccacgacc accggcaggt agccgcggaa gcgggtggct
     4021 agggtggcgg ggacgggcgc ggcggaggcg gcgctggggg cggggccggg gtcgatcatg
     4081 gcgggaagcc tagcaaatcg gggctcgccg gtcgctggat ggcgcggttg cgctgtgtgg
     4141 gagtggcgcg aggcgcggct gcgacagcgc aactacgccg gaacctgcgc cacagttgcg
     4201 ttgtcgcggt cgcggctcgc gccgctccta caggggcttc gggttagaaa tacaccacgc
     4261 cggtggcgcg catctgccgc agcaaggcgg cgcccatgtc caggaacgtg gcttcgtcgt
     4321 cgctgccggc ttcggccgcc agtgcgcgca gttgctcgcg ggcgatcagt tgcggcgact
     4381 cttccagccg ttgcagcagg cgataggcca gcgggctcag ttcggaaaag cgcacctcgc
     4441 cgtcggcctc gcgccgcagc agcagcaagg tcggcgtcgg cggcggttgc gcgggctgga
     4501 agtccgggcc gagccggtgc accggccagg cgtaggccag cggccaggcc agcggcgaca
     4561 ggcgcggcac gtgttcgagc aggccggcgt cgccgatgcc ggcgtcgtcg cgcgcggcgg
     4621 aggcttcgct gagctgcagc gccagctcga cccattcgta atgcgccagc tccagcagga
     4681 acggcgggtc gagcgcgtcg agattctgca ggtagcgctg gaactcgcgc gcgatctcgg
     4741 cgaacagcgg cgtgtggcag cgatggtcgc ggtagaaatc gcgtaccaac cgctcccagc
     4801 gttcctcgcc gagaatgctt tcgatgaccg gaaaattgcc cgacaacagg ctggcgatgt
     4861 tgttgaacag caggtcgcgg taaatgctca ggcggcgatc ttcgatgccg gccggcgcag
     4921 ggctggcgga cggatcgcgc aagtgtcggg tcagttcgaa ctgttgcgcg cgcaggcgcg
     4981 aggaggcgtc gttcatcggc tcaggcgtgg gcgcggcggg cggagtcgcg cgcggccggc
     5041 gcggcggatt cgcgcaggat gcggcgcacc aggcccagtt cgtcgaccag ttcggcgtag
     5101 ggcgggaaat tgaagtcgcg ttccagcagg gtcgggcgcg gcccgaacag gcggtaggcg
     5161 tcggccaaca gatcccagac ttcgcgcttg accggcgcgc cgtgggtgtc gaccttcaga
     5221 tcgtcggcct cgtcgtaatg gccggcgacg tgcagatagg cgatgcgctc gtgcggcatc
     5281 gcgcggatga attccagcgc gtcgtagcgg tggttgatcg agttgacgta gacgttgttg
     5341 acgtccagca gcaggtcgca atcggcctcg gccagcaccg cgcgggtgaa ctcgatctcg
     5401 ctcatttcct ggtgcggcgc ggcgtagtag gaggcgttct ccaccgcgat gcggcggccg
     5461 acgatgtcct gggtgcggcg gatgcgcgcg gcgacgtggt gcacggcttc ctcggtgaac
     5521 ggaatcggca acaggtcgta gaggtggccg tcgtcgctgc aatagctcag gtgttcgctg
     5581 tagcaggcca cggcgtgttc gtcgaggaag cggcgcacgc ggccgaggaa tgtctcgtcc
     5641 agcgcagcgg tgccgcccag cgacagcgac aggccgtggc agaccagcgg atagcgcgcg
     5701 gacagctcgc gcagttgctc gccgtagcgt ccgccgacgc cgatccagtt ttccggcgcg
     5761 agttcgagga aatcgaaatc gcgcgcggcg ccgggcgcgt gcggcgcgga ctgcagcggg
     5821 cccagcaggg cccgccgcag gccgaggccg acggcatccg gagtcagcga atgcgtgcgg
     5881 tccacggtgg tgccgcgatt acatcgaacc gccgcacttg ccctcgccgc acttgccttc
     5941 gcccgccttc ttggcggccg gggcagcgcc cttcttgtcg gcgccgcact tgccctcgcc
     6001 agtcttcttg tcggcgccgc acttgccttc gccgcacttg ccttcggcgt gcttcttctt
     6061 gtccgcgccg cacttgccct cggctttctt cttgtcggcg ccgcagctgc cttcggcctt
     6121 cttggccgcg tcggtggcct tggcgtcggc ggccttctcg gcggtctgcg cctgctgggc
     6181 gccgagcagg tagccctgcg acaggtcgct catcgcgaac gcggaggacg acaggctcag
     6241 gccgccgagc agggtggcgc tcagcgcgag ggcgatgggc ttgttgattt gcttggacat
     6301 gggttctctc ctggtggcga cgggcgggtg ctccgccggt gttggtagac gggcaggacc
     6361 cgccgggaaa cgcgattggt gcgcagctta gatcgattgc gggttagtgc ggggtgacat
     6421 ccgtaacggc ctggacagtt gccgggcctg ggcggcggtg cgcgcaggat ggctactgga
     6481 cgggaatacg gcgggggccg cggcgcggat gcagctcacg gcgacagata ttcgcgggcc
     6541 agcacccggg cgcggtgcag gcgcgatttg accgcctcgc ggctcaggct cagctcctcg
     6601 ccgatctcgg ccacggtcag gccttccagg tcgcgcagca ggatgatctg gcggtagtgc
     6661 gcgggcagcg actccagggc gacggcgaca tcgtggcgcc attcggccgg ttcgggggtg
     6721 accaccggtg cgatctcgtc ggtgatcggc tggccgctga gcaggcgccg gccgcgcttg
     6781 agccggttgc actcgcgctt gacgatgcgg aaggtccagg agacgaagca ctcgagcacg
     6841 cgcaggtcgc gcagcttgcg cgaaaccgcg atcaggcttt cctgcaccgc gtcctcgatg
     6901 tcgttgatga cgcagtggaa ttcggcgtag cggcgcaggt cctggcgcga gtgccgcagc
     6961 acccgttcca gggcggccgt gtcgcccgcg cgggcggcgg ccagatcgcc attggccagt
     7021 aacggcgtca tgtcggggcg gttcctctct tggtccgtga aaatacaccc ggcgaagggt
     7081 gcgggcatgg atcaagaggg ggctggggcg cgcacggatt cgcgccgggg ccttgctacg
     7141 gcggcaaggt ttaccgggtg ttcagcgcgg cggcgcggtg gaaacgattg caggcggatc
     7201 cggcgatggc ggcgcggacg ttgccgccga cggacggcgc ggcagtcccg gccgcatgct
     7261 gcggcgcagc gcgaggccgg cgcgcgcggc caagtgacgg cgaggtgacg gccctgcgaa
     7321 acggcgccgg cggcgcaaac gaaacggccc gccgcgatgc cgcggcgggc cggactcagc
     7381 gggacggcgg gcgcgcgcgg cgcccggcgc tcacaccgac gcggtggtgt tggtggtgtc
     7441 gcgcttctcg cgcggcggca gcggctcgtc gcccttgacc aggaaccaca cgttctcggc
     7501 gatgttggtg gcgtggtcgc cgacgcgctc gatgttcttg gccatgaaca gcagatgggt
     7561 gcaggcggtg atgctgcgcg cgtcttccat catgtaggtc agcagctcgc ggaacaggcc
     7621 ggtgtaggcg gtgtcgacct cggcgtcgcg gtcgcgcacc cgctgcgcca gttcgatgtc
     7681 gttgtcgcgg taggccacca gcacatcgcg cagctgcttg actgccagcg tgcccagcgc
     7741 gtgcaggccg gccacgtgcg gcagtggcgg cgacaggttc agcgcggtcg agcgcttggc
     7801 cacgttggcg gcgtagtcgc cgacgcgctc gatgtcggag gcgatgcgga tcgcggccag
     7861 gatctcgcgc aggtcgcgcg ccatcggccc gcgcagcgcc agcttcatca cgtcgtggct
     7921 gatctcctgc tccagcgcgt cgatcgcctc gtcgttggcg atgatgcgct cggcggcctt
     7981 gtcgtcgcgg cgctggacca cgtcgagcgc ggattccagt tgcgcggcgg cgatctcgcc
     8041 catgcgcagg gtttcgtcga gcaggcggcg ctgctcgtcg tcgtagcttt tgacgatgtg
     8101 gtcgtgcatc tggttgctca tgagtggtgt cctgattgcg tttttccttc tcccgctggc
     8161 gggagaaggt gcccgcaggg cggatgaggg cgcgcggcga cgtcgccctc accccaaccc
     8221 ctctcccgcg ggcgggagag gggctcgtag ggatggctca gccaaagcga ccggtgatgt
     8281 agtcctcggt ctgctgcttg gtcggcttgg agaagatctg ctcggtcggc ccgtgttcga
     8341 tcaggtcgcc caggtacatg aaggcggtga agtcggacac gcgcgcggcc tgctgcatgt
     8401 tgtgggtcac gatggcgatc gtgtagtcct tcttgagctc ttcgaccaac tgctcgatgc
     8461 ggctggtgga gatcgggtcc agcgccgagg tcggctcgtc gagcagcagc accgagggct
     8521 tgagcgccac cgcgcgggcg atgcacagac gctgctgctg gccgccggac aggcctagcg
     8581 cgctctggcc gagcttgtcc ttgacctcgt cccacagcgc gccctggcgc agcgcgtgct
     8641 caacccggtc gttcatttcc gacttcgaca gcttctcgtg gtggcggatg ccgtaggcca
     8701 cgttctcgaa gatggtcatc gggaacggca ccggcttttg gaacaccatg ccgaccttgc
     8761 tgcgcaggcg gttcatcggg tacttcgggt cgaggatgtt gtccccgtcc agcagcacct
     8821 ggccgcgcgc ctccagcttc gggtacagcg cgtagatgcg gttgaagatg cgcagcaggg
     8881 tcgacttgcc gcagcccgac gggccgatca gcgcggtgac ctgcttttcc gggatttcca
     8941 ggttgatcga cttgagcgcg tggaacttgt cgtaatagaa attcagatcg cgcgcggcca
     9001 gcttgaccgg gttggcgccg accgcttcgc ggccgggcgt ggcgacggac aggcgtgcgt
     9061 cggacgaacg agcgtcattc ataaccgggt tccgggcgtc agcgggagtg ttggagaggt
     9121 cagtcattgg aaatccgatt gcgcagcacc agcgcgcgcg cgagcaggct tacgacgagg
     9181 acgaagaagg tcagcaccag cgcgccggcc caggccaggg tctgccaggt ttcgtagggg
     9241 ctgccggcga actggttcat caccaccggc acgctcgcca tcggctgcag cacgttgtgg
     9301 ctccagtact ggttgccgaa ggcggtgaac agcaacggcg cggtttcgcc gctgatgcgc
     9361 gccagcgcca gcagcacgcc ggtgacgatg ccggccgaag cgctgcggta cagcacctgc
     9421 atgatcacct tccactgcgg cacgcccagc gacagcgccg cttcgcgcat ctgcggcggg
     9481 accaggcgca gcatctcgtc ggtggtgcgc accaccaccg gcagcacgat gaaggccagg
     9541 gcgatcgcgc cggcgagcgc ggagaagttg ccgccggtct gcatcaccac cagggtgtag
     9601 acgaacaggc ccagcacgat cgacggcgcc gacagcagga tgtcgttgac gaagcgcacc
     9661 accgtgccca tcttgcgccc ggcgccgtac tcggccagcc aggtgccggc ggcgatgccg
     9721 agcggggtgc cgatgacgat ggcgatcaag cacatcaccg cgctgccgaa gaaggcgttg
     9781 agcaggccgc cttcctgcat cggcggcggc gtgttctggg tgaacagcgc caggttgatg
     9841 ccgccgatgc ccttggagat cagggtgtag aggatccagc cgaggaagaa caggccgaac
     9901 agcgcggcgc cgcaggccag cagcaccgag acggcgttga ctacgcggcg gcggcggtac
     9961 agcgaatcgg cggtgcgatg gcgcgcggct tcggccgcgt cgtgcttgct ggcgacggcg
    10021 ctcatcagtt gccctccttg cgcgcgagtt gcatcagcat cagccgcgct gcggccaaca
    10081 ccacgaaggt cacgatgaac aacacgaagc cgagcagcag cagggccgag cggtaggtct
    10141 cggtggcctc gccgaagtcg ttggcgatca gcgcggcgat ggtggtgccc ggctccagca
    10201 gcgaagcgga gaagttcacc gagttgccga tcacgaaggc caccgccatg gtctcgccga
    10261 gcgcgcggcc caggccgagg aagatgccgc cgatgaccgc cgagcgggtg tagggcagga
    10321 cgatgtccca gctgacttcc cacttggtcg agcccagcgc gtaggccgat tccttgagcc
    10381 gggtgggcac ggtcaggaac acttcgcgca tcaccgagga gatgaaggga atgaccatga
    10441 tcgccaggac gatgccggcg gtgagcatgc cgatgcccag cggcgggccc tggaacagcg
    10501 cgccgatcac cggccaggtg ccgacgttgt cgttgagcca gggcgtgacg tattcggtca
    10561 tcaccggcac cagcacgaac aggccccaca tgccgtagat gatcgagggg atgccggcga
    10621 gcagttcgat cgcggtgccg atcgggccgc gcgcccagcg cggcgcgacc tcggtgagga
    10681 agaaggcgat gccgaagctc accggcacgg cgatgaccat ggcgatcagc gcggtgacga
    10741 tggtgccgta gatcggcacc agcgcgccgt agtggttttc gaccgggttc cactgcgcgg
    10801 tgatgaagaa gtcgatgcct tcttcttcca gcacgtggcg gccgccccac agcatcgaca
    10861 gcgcagcgct ggccagggcg atcag
//
