LOCUS       NZ_LT629789            13376 bp    DNA     circular CON 13-MAY-2024
DEFINITION  Amycolatopsis keratiniphila strain FH 1893 chromosome I.
ACCESSION   NZ_LT629789
VERSION     NZ_LT629789.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis keratiniphila
  ORGANISM  Amycolatopsis keratiniphila
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis; Amycolatopsis japonica group.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900105855.1-RS_2024_05_13
            Annotation Date                   :: 05/13/2024 02:01:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,886
            CDSs (total)                      :: 8,813
            Genes (coding)                    :: 8,742
            CDSs (with protein)               :: 8,742
            Genes (RNA)                       :: 73
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 51 of 71
            Pseudo Genes (internal stop)      :: 1 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:07:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8999236
            Orig. end                         :: 9012612
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT629789.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13376
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="aminopolycarboxylic-acid"
                     /region_number="35"
                     /rules="(AesA and AesB and AesC)"
                     /tool="antismash"
     cand_cluster    1..13376
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(AesA and AesB and AesC)"
                     /kind="single"
                     /product="aminopolycarboxylic-acid"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13376
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8376](+)"
                     /cutoff="10000"
                     /detection_rule="(AesA and AesB and AesC)"
                     /neighbourhood="5000"
                     /product="aminopolycarboxylic-acid"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8376
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(AesA and AesB and AesC)"
                     /neighbourhood="5000"
                     /product="aminopolycarboxylic-acid"
                     /protocluster_number="1"
     misc_feature    complement(1025..1039)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.13"
                     /tool="antismash"
     gene            1163..1579
                     /locus_tag="BLU53_RS41465"
                     /old_locus_tag="SAMN04489733_8501"
     CDS             1163..1579
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336503.1"
                     /locus_tag="BLU53_RS41465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8501"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_072030587.1"
                     /transl_table=11
                     /translation="MDDSYRLEAGSPSVDDYLRLRREAGLSEPPREQAERGVGGAWASV
                     RVVHRTSGEIVGMGRVISDGGWYFHIVDMAVLPAHQRRGIGDAVLEALLAAIDAEAPGA
                     YVNLLGDPPGWRLYKRHGFVETAPGTIGMRRQNT"
     gene            complement(1632..2156)
                     /locus_tag="BLU53_RS41470"
                     /old_locus_tag="SAMN04489733_8502"
     CDS             complement(1632..2156)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336502.1"
                     /locus_tag="BLU53_RS41470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8502"
                     /product="cysteine dioxygenase family protein"
                     /protein_id="WP_063273637.1"
                     /transl_table=11
                     /translation="MTVTTLRPGLPELIKRLSQCTERPSEPLVTAQAVATVLTELRPTP
                     RLLTTAERAGSPEGYTRTTLHAESAFSIVALVWRPGQLTEIHDHLVWCTFLVLQGTETE
                     TIFDIDGDRLVRTAQRQRPAGSVSGVAPPDDIHQVHNAGDTVAITLHVYGADLSKGTSV
                     RRNYRMRATAR"
     misc_feature    2234..2249
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.58"
                     /tool="antismash"
     gene            2251..3117
                     /locus_tag="BLU53_RS41475"
                     /old_locus_tag="SAMN04489733_8503"
     CDS             2251..3117
                     /GO_function="GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630847.1"
                     /locus_tag="BLU53_RS41475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8503"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_016336501.1"
                     /transl_table=11
                     /translation="MLNLVQLTVLAAVARHGSMTAAAKELHYTQPAVSHHLARLEAATG
                     AKLVQRIGRGIRLTPEGELLAARAAEIVGRVEGAEAELAAQVGLRAGRVRMAGFQSILS
                     TIVPDAAAALARAHPGLELGLVDEHPGEALRMLREGRIDVALIFRYADTPREERGLRLV
                     HLLEDPIYLLSSEPGQTIADHRDSTWIGGCTRCQDELVTICGRAGFAPRISLVSDDIVV
                     MQALVAARMGVTTLPRLALRAHRMPGVHATELAEDPRQIYAVTYGDPPDPPAAAALIDA
                     LQASIRA"
     gene            complement(3617..4900)
                     /locus_tag="BLU53_RS41480"
                     /old_locus_tag="SAMN04489733_8504"
     CDS             complement(3617..4900)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1105: amidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159695.1"
                     /locus_tag="BLU53_RS41480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8504"
                     /product="amidase family protein"
                     /protein_id="WP_228708176.1"
                     /transl_table=11
                     /translation="MSRPWSVAESRARAAEMRHLNAIGPLAPHDHAAAPRGPLSGIPLV
                     VKDNIHVAGMPNTAGTPALADHVPSEHATVVRRLTDAGAVVVGKATMHELALGITCDTT
                     PLGPVRNARDPSRFAGGSSGGTAVAVAAGIVPAGLGTDTGGSARVPAALNGVCGFRPTT
                     GRYPSDGMTPLSSTRDTTGPIARTVADLALLDAVLAAEEPAPLIERPSIRLGVPHGFLT
                     GDLSEDVEELWEAALARLGAAGVTLVPLDDTPLAELVVDQGMPLVIHEAGVGLRSYLAE
                     HVPEVSFEQLVREIAAPDVRAIFTEAVVPGVEPAVYETALTTRSALRRAYAKIFDESGI
                     DALAFPTTPATARDFSAVGSFVHRGREVPTFATFIRNCQPGSIAGFPGLTVPMGRARDG
                     LPAGLALDGLVGEDRKLLAVGAFVERAL"
     misc_feature    complement(4936..4951)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 20.59"
                     /tool="antismash"
     misc_feature    4939..4954
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 22.59"
                     /tool="antismash"
     misc_feature    4941..4955
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.98"
                     /tool="antismash"
     misc_feature    4942..4955
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.33"
                     /tool="antismash"
     misc_feature    complement(4942..4957)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: medium, score: 24.15"
                     /tool="antismash"
     gene            5001..5984
                     /locus_tag="BLU53_RS41485"
                     /old_locus_tag="SAMN04489733_8505"
     CDS             5001..5984
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     aminopolycarboxylic-acid: AesA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1158: ornithine cyclodeaminase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630845.1"
                     /locus_tag="BLU53_RS41485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8505"
                     /product="ornithine cyclodeaminase"
                     /protein_id="WP_063273634.1"
                     /sec_met_domain="AesA (E-value: 1.9e-158, bitscore: 525.2,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSGPSLHVLTRSDLHSLELTPAEAITMVEDGYLAYAAGASRNPA
                     KLMVPMPDPARDAVAYSMLGYDGSLEQAAFKTSYRQGSTSAEKYYTTITLYDDTTGLPF
                     ALMDCHRVGATRTPASTALIARSCARPGARSALMVGTGAQGIRTLPYLLTALPELERLR
                     LFGTHPDGLRDSVAAVKERFPDREVELVDDVEAAARESDIVVAASGRAAHPKIRLGWLP
                     PGGLLISVASKGVQEGTLAEADYTVATSGAQVEVTGQRMAGPDGVFRIDAELPEILAGK
                     VPGRRGDDDRVFAFSSGMIITDIPVAHALAARAIAAGRGREVALWT"
     gene            5975..7321
                     /locus_tag="BLU53_RS41490"
                     /old_locus_tag="SAMN04489733_8506"
     CDS             5975..7321
                     /EC_number="5.1.1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     aminopolycarboxylic-acid: AesB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630844.1"
                     /locus_tag="BLU53_RS41490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8506"
                     /product="alanine racemase"
                     /protein_id="WP_072030589.1"
                     /sec_met_domain="AesB (E-value: 5.9e-214, bitscore: 709.2,
                     seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 5.5e-16, bitscore:
                     57.7, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLIDTLSPPLPALERPWAHRLRDSETLWDIARGVGGGPFHVVHP
                     ATFSENLGGMVDALAVERVEGTVYYGKKANKAASWLRECTRPGTGVDVASVPELVHALG
                     NGLRGESIGVTGAAKPDGLLWLALRHRCLIAVDAADELERVARLATEIGETAEVLLRVR
                     PPSAPESRFGFAPDALKAAVRQCGGPVALRGFSFHLDGYDPVPRAELAAYLIDLCHDAR
                     ALGHPASKISVGGGIAVSYVDAADWVRFESGRHDGWFHAGRNPAKTYPYHQAPTGAAMV
                     TAILRHEIAGKTLAERLRAAGIELLLEPGRALVDGAGFSVFPVLGCKPAEDHLITTVAG
                     LSMSLSEQWKGSEFLPDPLLVRRDGPGGTPVRTIVAGSSCMEYDVLTWRAVELPARPRT
                     GDLLVYPNTAGYQMDKNESGFHQLPLPPKVVVDGDRWHLDTDHPIQEIR"
     gene            7318..8376
                     /locus_tag="BLU53_RS41495"
                     /old_locus_tag="SAMN04489733_8507"
     CDS             7318..8376
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /GO_process="GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     aminopolycarboxylic-acid: AesC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336497.1"
                     /locus_tag="BLU53_RS41495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8507"
                     /product="cysteine synthase family protein"
                     /protein_id="WP_072030590.1"
                     /sec_met_domain="AesC (E-value: 4.4e-173, bitscore: 574.1,
                     seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="PALP (E-value: 3.4e-56, bitscore: 189.8,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIVRRASDLIGNTPLLELARTGTGTRLLLKLDHLNPTGSCKVRM
                     ARQMIDEAELDGRLRPGGHIVEPTSGNTGNGLALVALERGYRFTAVVDHHAAREKLRML
                     RALGAELVFVECPPDGGVSSVQRRRVAARIAAETGAYHPDQHNHPGNGNGYTGLARELL
                     GQLGDVDVLVAAIGTGGSLCGTARALRAAGAATHAVAVEPVGSIIFGGAPGIYHQTGAG
                     SPAGFPIGDNVDRSVIGEAHRVSDSDAFAGARVVARRTGVLVGGTTGGAIHVALRRLYA
                     YPAGSVVVVLCNDAGEKYLDSVYDDEWLRARGVLDELAHRRMHGWFATYAESVRVATRD
                     RAVRRTPVAVGG"
     gene            8373..9755
                     /locus_tag="BLU53_RS41500"
                     /old_locus_tag="SAMN04489733_8508"
     CDS             8373..9755
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /GO_function="GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1086: MATE efflux
                     family protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031524.1"
                     /locus_tag="BLU53_RS41500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8508"
                     /product="MATE family efflux transporter"
                     /protein_id="WP_072030591.1"
                     /transl_table=11
                     /translation="MSTAMTTRGYRALAGLAAPIAGIQLAQVALTSVDLAMLGLLGVTA
                     VAAGGLAILLYNQIRTMCVGMVTGVGNLVATAAGTGELRTGTGELDERARDEIRSLLRS
                     ALLVATLTAAIGAAVLCGLAVALPLLGQKPEIVSLAGPMMFALAGGLFPMVWLNVVRQF
                     AVGLRRPGSLLAVTLVSIAVNAALNAAFIYGWAGLPELGVAGIGLATTLVQFFTLAVFA
                     SALRRDPLLRPMVSLALWRADPAVVRRIVRHGTPICFTYGSEAAITSIATMLMGAFGPA
                     MLAASNVANQLAYIVYQVNIGLSQGSSILVSRAVARDERWRAPVIARQAFTLSWSLMAV
                     VSLAYLLVPQVLLWPFLHDEADAVVLGTASTLLVFAVAQQYSKGTQNILVGLLRGLGDT
                     VSGLRCTLVGYWAVGVPAMFLCAYVFAWGGWGVWAGLCLGFAATAVLLGRRFAGQGFGS
                     AR"
     gene            complement(9638..10672)
                     /locus_tag="BLU53_RS41505"
                     /old_locus_tag="SAMN04489733_8509"
     CDS             complement(9638..10672)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336495.1"
                     /locus_tag="BLU53_RS41505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8509"
                     /product="hypothetical protein"
                     /protein_id="WP_228708177.1"
                     /transl_table=11
                     /translation="MTTPGRIGIAEVECLENAVDGLRALDYRQGGAFSRDAVHVVKSVS
                     LLLNWGAVPGGLRSRLLTVLADLHNLLGWTEFDIGRRVPARLRFDRALGLAAAAGNDDL
                     MANICYRLGRLALHHDDIEGGLDHLRQGQAAAHRSDSPRAQAILTVNEAWALAKAGDER
                     AALTSLAHAECQFENAGNWPSPRPWEAFFGTTDMAAMRGTVLTELARTVTSRHCEEAIA
                     RLHDAVGGYGAEMSRSLALTLIMLAQNHALQGDFDEAVRTGTEALDLARGLGSTRTKDR
                     LAPLAALLRGHADPASRDLLARIDGYRAEPNPWPANRRPSRTAVAANPRHRPAQTPQPP
                     QANT"
     misc_feature    10666..10682
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.72"
                     /tool="antismash"
     misc_feature    complement(10761..10774)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 20.67"
                     /tool="antismash"
     gene            10799..11221
                     /locus_tag="BLU53_RS41510"
                     /old_locus_tag="SAMN04489733_8510"
     CDS             10799..11221
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1266: hydrogen
                     peroxide sensitive repressor"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031522.1"
                     /locus_tag="BLU53_RS41510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8510"
                     /product="Fur family transcriptional regulator"
                     /protein_id="WP_037341998.1"
                     /transl_table=11
                     /translation="MSDFEAQLRAVSLRVTRPRLAVLAALRDHPHVDTETVITLVRADL
                     PTVSHQAVYDVLRALTETGLIRRIQPAGALARYETRVGDNHHHVVCRSCGAIADVDCAV
                     GHAPCLTASGDHGFVIDEAEVVYWGLCPGCAAEPVQ"
     misc_feature    complement(12876..12890)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.92"
                     /tool="antismash"
     misc_feature    13218..13233
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 20.5"
                     /tool="antismash"
     misc_feature    13341..13356
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.42"
                     /tool="antismash"
ORIGIN
        1 gtcgtctcca gccgtggcaa cgggttgccg cccggccgga accgcagggc gcggaacagg
       61 tcccgcctgc tgccgaagcc cagcttctgc aggatccggc tgacgtagtt cttcacggtc
      121 tgcgtcgcga ggtgcagttc ttcggcgatc tcgtcgttcg accaccggta gaggatcagc
      181 gcgaggatct cctcctcccg tctggtcatc gcgctcgcgt agtgcacggc ctcggtgacc
      241 ggcggcggcg ttccgcccgc ctgcacgcac acccgctggc cggcgagcgt ggagcgcacc
      301 gccgacatca cctggccgcc gctggccgat ttgtgcacga agctgtccgc gcccgcgttc
      361 agtgccatgg cgatggccga tggcgacgaa tcaccggcga agaccaggac ggtcgcccgt
      421 tcgaactgtt ccttcacgaa ccggcagatc gcggtcccgg cgggctcacc ccagatcgac
      481 agctcggtga tgaccatgtc gggatggcgt ttttcgcact gccacaacgc ttccggctcg
      541 ctcgcggctt cggccaccgc ccccaccccc gccgaatccc gcaacagcct gctgagcgcg
      601 tatctgatga ggggttgatc gtctactacg agcgtggtga cgcggttcat ggttcccctt
      661 ggggtatcga cgggcgcccg gtgtggcccg ggccgtgcgg tcggccccgg gccgggcggc
      721 gctcgcattc gccgagaaca ggttcgctcg ctcgggcgat ccgtcgaacc gaccgtcgcg
      781 gcgacgtcgg tactcatttt tcgccgaatc ctctttcgtc gcggtccggc agcgatcttc
      841 ccgcttatcc gcgcgagtga ggaccagcaa tcgggtgaca acggcgaaaa gcggccacgc
      901 ggggcgccgg atctgggccg gacggcggaa cggggataca gattccgaac gcgccgtact
      961 ccagacgtgg aaagccgggc cgacttgtcc gatcgtgtga catacgggcc cgatatcgcc
     1021 ttcgtgaact cgatttcccg ggtccggtat tcggcggggt ccattgtgga ggccgggctc
     1081 cgtcgggggc ccggcctccg gacgcgcgcg caccgggtgc gacgctgtag gccgttggtg
     1141 tgagagaaag ggcaagcccg tcatggacga ctcttaccgc ctggaggcag gctccccgtc
     1201 ggtcgacgac tacctacggc tgcgtcgcga ggccgggctc agcgaaccgc ccagggagca
     1261 ggcggagcgc ggggtcggcg gcgcttgggc gtcggtccgg gtcgtccacc ggacctccgg
     1321 cgagatcgtc gggatgggcc gggtgatcag cgacggcggg tggtacttcc acatcgtcga
     1381 catggccgtc ctgcccgccc atcagcgtcg aggtatcggg gacgccgtcc tcgaagccct
     1441 tctcgccgcg atcgacgccg aggctcccgg cgcgtacgtc aatttgctcg gcgatccgcc
     1501 cggatggcgg ctctacaaac ggcacggctt cgtcgaaacc gctcccggga ccatcggtat
     1561 gcggcggcaa aacacctgac cggcctcgat ggggatgcca ataggcgaaa tgggcccatc
     1621 gattgcgcct ttcaccgagc ggtggcgcgc attcggtaat tgcggcgtac gctcgttccc
     1681 ttgctgaggt cggcaccgta cacgtgcaag gtgatggcga cggtgtcgcc cgcgttgtgc
     1741 acctggtgga tgtcgtcggg tggggcgacg ccgctcaccg agccggccgg gcgttgccgc
     1801 tgggccgtcc ggaccagcct gtcaccgtcg atgtcgaaga tggtctcggt ctcggtccct
     1861 tgcaggacaa ggaatgtgca ccacaccaga tgatcgtgga tctcggtgag ctggccgggc
     1921 cgccagacca gcgcgacgat cgaaaaggcg gactcggcgt gcagtgtggt gcgggtgtac
     1981 ccctcggggg agccggcgcg ttccgccgtg gtgagcagcc gcggagtcgg ccggagttcg
     2041 gtgagaacgg tcgccacggc ttgtgcggtg accaggggtt cggacggcct ttcggtgcat
     2101 tggctcagac gttttatcag ctccgggagg ccgggtctga gagtggtcac ggtcattttc
     2161 gtcctccttc tcgtcgcttt tcatcgtggc ccgctgtccg ggcgtcgtcc aatgacgacg
     2221 ttctttcttt ttcataggta ttctttatcc atgctgaatc tggtgcagct caccgtgctg
     2281 gcggctgtcg cccggcatgg ttcgatgacc gcggccgcga aggaactgca ctacacgcag
     2341 ccggcggtga gccaccatct cgcgaggttg gaggcggcca cgggcgcgaa actggtccag
     2401 cggatcggcc gcggcatccg gctcactccg gaaggggaac tgctcgccgc ccgcgcggcc
     2461 gagatcgtcg gccgggtcga aggcgccgag gccgagctgg cggcgcaggt gggcttgcgc
     2521 gccgggcggg tccggatggc gggcttccag tcgatcctga gcaccatcgt ccccgacgcg
     2581 gccgccgcgc tggcccgtgc gcatcccggg ctcgaattgg ggctggtgga cgaacatccg
     2641 ggcgaggcgc tgcgcatgct gcgggaaggg cggatcgacg tcgccctgat cttccggtac
     2701 gcggacacgc ctcgtgagga gcgggggctc cggctggtgc atcttctgga ggacccgatc
     2761 tacctgctca gcagcgaacc gggccagacc atcgccgacc atcgtgactc gacctggatc
     2821 ggcggttgca cccgctgcca ggacgaactc gtcacgatct gcgggcgggc cgggttcgcc
     2881 ccgcggatct cgctggtcag cgacgacatc gtggtcatgc aggcgttggt cgcggctcgg
     2941 atgggcgtca ccacgctgcc gcggctggcg ctgcgcgcgc accggatgcc cggtgtccac
     3001 gccaccgaac tggccgaaga ccctcgccag atctacgccg tcacctacgg ggacccgccc
     3061 gacccgccgg cggcggccgc gctcatcgac gcgcttcagg ccagcatccg ggcttagcgc
     3121 gggacttggg gaaggtgtga aggccccctt tcctcggctg agccgaggga agggggcctt
     3181 cacgcgcggc cgtggtgatt gctgtggttg ctggtgcagg tgagctgcag gtgttcgcta
     3241 ccttcagggt caaggttccg gcaaagtcgc ccattcgctg gtcaagtggg caacggaggc
     3301 actcgccacg gtggcctgac tccttctcga ctgtccacag gggacccttc cagggtgaga
     3361 gtgcccgatt tgggcgtttc gcaaaggggt cgtgagtggt aaagagggtt agaacgacct
     3421 ttaccactca cgacccctcg gcataatcac ccatttcgcc atgaatggga cattcggtcg
     3481 ccgagaagtg accacagtgg acggttggag cccgttcgac tccggtcgac cagggtaatc
     3541 aagcgcttga cctgcgctta tgtgactttg ccggggctct gaccttcagg gtgggcaagg
     3601 aggccttcac ggacagctac agagcccgtt cgacgaaagc ccccacggcc agcagtttcc
     3661 ggtcttcgcc gaccagcccg tcgagcgcca gtccggcggg aagcccgtcg cgtgcgcgcc
     3721 ccatcgggac ggtcaggccc gggaatcccg cgatgctgcc gggctggcag ttgcggatga
     3781 aggtggcgaa ggtcgggacc tcgcgcccgc ggtgcacgaa actcccgacg gccgagaagt
     3841 cccgcgccgt cgccggggtg gtggggaagg ccagcgcgtc gatgccgctc tcgtcgaaga
     3901 tcttcgcgta ggcgcggcgc aaagcggatc gggtggtgag tgccgtctcg tacacggcgg
     3961 gctcgacgcc cggcacgaca gcttcggtga agatcgcccg gacgtcgggc gcggcgatct
     4021 cgcgaacgag ctgctcgaac gagacctcgg gtacgtgctc ggcgaggtag gaccggagcc
     4081 cgacgcctgc ttcgtggatc accagcggca ttccttggtc gacgacgagt tccgcgagtg
     4141 gtgtgtcgtc gagcggcacc agggtgacac ccgccgcgcc caggcgggcc agtgcggcct
     4201 cccagagttc ttcgacgtcc tcggacaggt cgccggtcag gaaaccgtgc ggcacaccca
     4261 gccggatcga cggacgctcg atcaggggcg ccggctcttc cgccgcgaga acggcgtcga
     4321 ggagcgcgag atcggcgacc gtgcgggcta tcgggccggt cgtgtcccgt gtgctgctca
     4381 gcggcgtcat gccgtcggac gggtagcggc cggtggtggg ccggaaaccg caaaccccgt
     4441 tgagcgcggc gggaacgcgg gccgagccac cggtgtcggt gcccagaccg gcgggcacga
     4501 tgcccgccgc gacggcgacc gcggtcccgc cgctgctgcc cccggcgaac cgggacgggt
     4561 cgcgggcgtt gcgcaccggg ccgagcgggg tcgtgtcgca ggtgataccg agggcgagtt
     4621 cgtgcatggt cgccttgccg acgacgacgg cgcccgcgtc ggtcagccgc cggaccacgg
     4681 tcgcgtgctc gctcggaacg tggtccgcca gcgccggtgt ccccgcggtg ttcggcatcc
     4741 ccgcgacgtg gatgttgtcc ttgaccacca atgggatccc gctcagcggg cccctcggtg
     4801 cggccgcgtg atcgtgcggc gccagcgggc cgatcgcgtt gagatgccgc atttccgccg
     4861 ctcgcgcgcg gctttccgcg acgctccaag gtctgctcac ggccgtcagc ttacgtgaca
     4921 aggcccacac tcatcgataa agacaatcat tttcattaag ctgcggcgcg ccaccttccc
     4981 acgagaaagg tcgatcgttc atgagctccg gtccctcgct gcacgtactc acgcgctcgg
     5041 atctgcacag cctcgaactc accccggccg aggcgatcac gatggtcgaa gacggctacc
     5101 tggcctacgc ggccggagcc tcgcggaacc cggcgaagct gatggtgccg atgccggatc
     5161 ccgcgcggga cgccgtcgcg tactcgatgc tcggctacga cggttccctc gaacaggccg
     5221 cgttcaagac gagctaccgc cagggcagca cgtcggccga gaagtactac acgaccatca
     5281 ccctgtacga cgacaccacg ggcctgccgt tcgcgctcat ggactgccac cgtgtcggcg
     5341 ccacccgtac gccggcgagc accgcgttga tcgcgcggtc gtgtgcccgg ccgggagcgc
     5401 gatccgcgct catggtcggc accggcgcgc aggggatccg gacgctgccg tacctgctca
     5461 ccgcgttgcc ggagctggag cggctgcggc tgttcggcac gcatcccgac ggtcttcgcg
     5521 acagcgtcgc ggcggtgaag gagcggttcc cggaccgcga ggtcgaactc gtcgacgacg
     5581 tggaggccgc ggcacgcgag tccgacatcg tggtcgccgc gtccggccgg gcggcccacc
     5641 cgaagatccg cctcggctgg ctgccgccgg gcgggctgct gatctcggtc gccagcaagg
     5701 gagtccagga aggcacgctc gccgaagccg actacaccgt ggccaccagc ggcgcgcagg
     5761 tcgaggtgac cggccagcgg atggcggggc cggacggtgt cttccgcatc gacgccgaac
     5821 ttccggaaat cctcgcgggc aaggttcccg gccgtcgcgg cgacgatgac cgagtgttcg
     5881 ccttcagcag cggcatgatc atcaccgaca tcccggtcgc gcacgcgctg gccgcccgcg
     5941 ccatcgccgc ggggcgcggc cgcgaggtgg ccctgtggac ctgatcgaca ccctgtcccc
     6001 gcctttgccc gcgctcgaac gtccctgggc gcaccggctg cgtgactcgg agacactgtg
     6061 ggacatcgcc cgcggtgtcg gcggcggccc gttccatgtg gttcatccgg ccactttttc
     6121 cgagaacctc gggggaatgg tcgacgcgct cgccgtcgaa cgtgtcgaag gcaccgtcta
     6181 ctacgggaag aaggcgaaca aggcggcgtc ctggctgcgg gagtgcacgc gtcccggcac
     6241 gggggtcgac gtcgcgagcg tgcccgaatt ggtgcacgcc ctcgggaacg gcctgcgcgg
     6301 cgagtcgatc ggggtcaccg gcgccgccaa acccgacggc ctcctctggc tcgccctgcg
     6361 gcatcgctgt ctcatcgcgg tggacgcggc cgacgaactg gagcgcgttg cccggctcgc
     6421 caccgaaatc ggggagacgg ccgaagtgct gctgcgggtg cggccgccgt ccgcgccgga
     6481 gagccggttc ggtttcgcgc ccgacgcttt gaaagcggcc gtccggcagt gcggcggccc
     6541 ggtcgccctg cggggtttct cgttccacct cgacggatac gaccccgtgc cgagggcgga
     6601 actcgccgcg tatctcatcg atctgtgcca cgacgcccgc gcgctcgggc atccggcgtc
     6661 gaagatcagt gtgggcggcg ggatcgcggt gtcctatgtg gacgccgctg actgggtgcg
     6721 gttcgagtcc ggcaggcacg acggctggtt ccacgcgggc cgcaatccgg ccaagacgta
     6781 tccgtaccac caggcgccga ccggggcggc gatggtgacc gcgatcctcc ggcacgagat
     6841 cgccgggaag acactggcgg aacgcctgcg cgcggccggg atcgagctgc tgctcgaacc
     6901 gggccgggcc ctcgtcgacg gcgccggatt ctcggtgttc ccggtgctcg gctgcaaacc
     6961 ggccgaagac cacctgatca ccacggtcgc gggcctctcc atgagcctgt ccgaacagtg
     7021 gaagggcagc gagttcctgc ccgacccgct gctcgtgcgc cgggacgggc cgggcggcac
     7081 gccggtgcgg accatcgtcg ccgggtcgag ctgcatggag tacgacgtgc tgacctggcg
     7141 cgcggtggag ctgcccgcgc ggccgaggac cggcgacctg ctcgtgtacc ccaacacggc
     7201 cggctaccag atggacaaga acgagagcgg cttccaccag cttccgttgc cgccgaaagt
     7261 ggtggtcgac ggcgaccgct ggcacctcga caccgaccac ccgatccagg agatccgatg
     7321 acgatcgtcc ggcgcgcgtc cgacctgatc gggaacacgc cgctgctgga gctcgcgcgc
     7381 accggcaccg ggacgcgcct gctgctcaaa ctcgaccacc tcaaccccac cggctcgtgc
     7441 aaggtacgca tggccaggca gatgatcgac gaggcggagc tcgacggacg gctgcggccg
     7501 ggcgggcaca tcgtcgaacc cacctccggc aacaccggga acgggctcgc gctggtcgcg
     7561 ctggaacgcg gctaccggtt caccgccgtg gtcgatcacc acgccgcccg cgagaagctc
     7621 cggatgctgc gcgcgctcgg cgcggaactg gtgttcgtcg agtgcccgcc ggacggcggg
     7681 gtcagttcgg tccagcggcg gcgggtcgcg gcccggatcg ccgcggaaac cggggcctac
     7741 caccccgatc agcacaacca tcccggcaac ggcaacgggt acaccgggct ggcgcgggaa
     7801 cttctcgggc agctcggcga cgtcgacgtg ctcgtcgcgg cgatcggcac cggcggctcc
     7861 ctgtgcggta cggcccgcgc cctgcgcgcg gcgggagcgg ccacccacgc ggtggcggtc
     7921 gaacccgtcg gctcgatcat tttcggtggc gcgccgggga tctaccacca gaccggggcg
     7981 ggcagcccgg ccggtttccc gatcggggac aacgtcgacc ggtcggtgat cggcgaagcc
     8041 caccgcgtgt ccgacagcga cgcgttcgcc ggggcgcgcg tggtcgctcg gcgcaccggg
     8101 gtgctggtcg gcgggaccac cggcggcgcg atccacgtcg cgctccggcg gctttacgcc
     8161 tatccggccg gaagcgtcgt cgtggtgctg tgcaacgacg cgggggagaa gtacctcgat
     8221 tcggtgtacg acgacgaatg gctgcgtgcc aggggagtgc tcgacgaact ggcccaccgc
     8281 cggatgcacg gctggttcgc cacctacgcc gaatcggtcc gggtggccac gcgcgaccgg
     8341 gcggtgcgcc ggacgccggt ggcggtgggc ggatgagcac ggcgatgacg acccgcggct
     8401 accgggcgct cgccgggctg gccgcgccga tcgcgggaat ccagctggca caggtcgcgc
     8461 tcaccagcgt cgacctggcc atgctcgggc tgctcggggt gaccgcggtg gcggcgggcg
     8521 ggctggcgat cctgctctac aaccagatcc gcaccatgtg cgtcggcatg gtcaccggcg
     8581 tcgggaacct cgtggcgacg gcggccggga ccggggaact ccgcaccggc accggcgaac
     8641 tcgacgaacg cgcacgggac gagatccggt cgctcctgcg gtcggcgctg ctcgtcgcga
     8701 ccctgaccgc ggcgatcggc gcggccgtcc tttgtggact ggccgtcgcg ttgcccttgc
     8761 tcggccagaa accggagatc gtgtcgctcg ccggcccgat gatgttcgcg ctggcgggcg
     8821 ggctgttccc gatggtgtgg ctcaacgtgg tgcgccagtt cgccgtcggg ctgcggcgtc
     8881 cgggttcgct cctcgccgtg acgctggtgt cgatcgcggt caacgccgcc ctcaacgccg
     8941 ccttcatcta cggctgggcg ggcctgcccg agctgggcgt cgccggtatc ggcctggcga
     9001 cgaccctggt ccagttcttc acgctcgccg tgttcgcctc ggccctccgg cgggatccgc
     9061 tgctgcgccc catggtttcg ctcgcgttgt ggcgcgcgga tccggcggtg gtccggcgca
     9121 tcgtccggca cgggacgccg atctgcttca cctacggctc ggaagcggcg atcacctcga
     9181 tcgcgacgat gctgatgggt gccttcggcc cggcgatgct cgccgcgtcg aacgtcgcga
     9241 accagctcgc gtacatcgtc taccaggtca atatcgggct gtcgcaggga tcgtccattt
     9301 tggtcagtcg tgcggtcgcc cgtgacgaac gatggcgggc gccggtgatc gcccgccagg
     9361 cgttcaccct ttcctggtca ctgatggcgg tggtgagcct ggcgtatctg ctggtgccgc
     9421 aggtgctgct gtggccgttc ctgcacgacg aggccgacgc ggtggtgctc ggcacggcgt
     9481 cgaccctgct ggtgttcgcc gtcgcgcagc agtacagcaa gggaacgcag aacatcctcg
     9541 tcggcttgct gcgcgggctc ggcgacaccg tttccggcct ccgctgcacg ctcgtggggt
     9601 actgggcggt gggtgtaccg gcgatgttct tgtgcgccta cgtgttcgcc tggggcggct
     9661 ggggagtctg ggccggtctg tgtctcgggt tcgcggcgac cgcggtcctg ctcggacggc
     9721 ggttcgccgg ccaagggttc ggttcggcgc gatagccgtc gatccgggcg agcaggtcgc
     9781 gcgacgcggg atcggcgtgt ccgcgcagga gcgccgccag cggtgcgagc cggtccttgg
     9841 tgcgggtgga accgagcccg cgtgcgaggt ccagcgcttc ggtgcccgtg cggacggctt
     9901 cgtcgaaatc gccttggagc gcgtggttct gcgcgagcat gatcagggtg agcgcgaggc
     9961 tgcgggacat ttcggcgccg tagccgccga cggcgtcgtg cagccgcgcg atcgcttctt
    10021 cgcagtggcg ggaggtgacc gtcctggcca attcggtcag gacggtcccg cgcatcgcgg
    10081 ccatgtccgt ggtgccgaag aaggcttccc aggggcgcgg cgacggccag ttgcccgcgt
    10141 tctcgaactg acactccgcg tgggccaggc tcgtgagagc ggcacgctcg tcgccggcct
    10201 tggccagtgc ccacgcctcg ttgaccgtca ggatcgcctg tgcccgaggg gaatcggagc
    10261 ggtgggcggc ggcttgaccc tggcggagat ggtccaaccc gccctcgatg tcgtcgtggt
    10321 gcagcgccag cctgccgagg cggtaacaga tgttggccat gagatcgtcg ttcccggccg
    10381 cggcggcgag accgagcgct cgatcgaacc gcagccgcgc cggtacgcgg cggccgatgt
    10441 cgaattcggt ccagccgagc agattgtgca gatccgcgag cacggtcagc agccgtgacc
    10501 ggagcccgcc cggaaccgcg ccccaattga gcagcaggct gaccgacttc acgacgtgca
    10561 ccgcgtcgcg ggaaaaggcg ccgccctggc ggtaatccag ggcacgcagg ccgtcgacgg
    10621 cgttttcgag gcactcgact tcggcgatac cgatacggcc gggcgttgtc atgaactcac
    10681 ctcacgcaag tcatggctcg atccacggct acccggtgtc aacgggttcg aaaccggccg
    10741 tcactccgcg gggttcttga tttgacccgt tccaaagacg ctcttatggt ccttggtgat
    10801 gtccgacttc gaggcacagc tgcgagcggt ttcgctgcgc gtgactcggc cccggctggc
    10861 cgtgctcgcg gcgctgcgcg atcatccgca cgtcgacacc gaaacggtga taacgctggt
    10921 gcgggccgac cttcccacgg tctcccacca ggcggtgtac gacgtgctgc gagcactcac
    10981 cgaaaccggt ctgatccggc gtatccagcc cgccggcgcg ctcgcccgtt acgagacccg
    11041 ggtgggggac aaccatcacc atgtcgtgtg ccgttcctgc ggtgcgatcg cggacgtcga
    11101 ctgcgccgtc ggccatgccc cctgtctcac cgcttcgggc gatcacgggt tcgtgatcga
    11161 cgaggcggag gtcgtctact ggggcctgtg ccccggctgc gcggccgaac ctgtccagtg
    11221 atgaattcgc ctgctcggaa ggaaagaaat gagtgacaac ccggagaagg gctgcccggt
    11281 ggcccacgac tccgtgaccg cgcacggaag cgagagcgag aacccggcga tcgactcgcc
    11341 gacgcccaag tcgggtggcc gcccgcgcac caacaaggac tggtggccca accagctcga
    11401 cctctcggtg ctgcacgtcc actcgtcgaa gtcgaacccg ctcggcgcgg acttcagcta
    11461 cgccgaggag ttcgcgaaac tcgacgtcga ggcgctcaaa cgggatatcg tcgaggtgct
    11521 caccacctcg caggactggt ggcccgccga cttcggccac tacggcggcc tgatgatccg
    11581 gatgagctgg cacgccgcgg gcacctaccg catccacgac ggccgcggcg gggccggtga
    11641 cggcgcgcag cggttcgccc cgctcaacag ctggcccgac aacgcgaacc tcgacaaggc
    11701 gcggcggctg ctgtggccgg tcaaggagaa gtacgggcag cggatctcgt gggccgacct
    11761 gctcgtgctc gccgggaacg tcgcgctgga gtcgatgggg ttcaagacgt tcggcttcgg
    11821 tttcggccgc gtggacacct gggagcccga ggagatcttc tggggtccgg aagacacctg
    11881 gctgggtgac gagcgctacg cgaccgacac cgagatggtc cccgacgtcg gcgcgaccga
    11941 gatgggcctc atctacgtca atcccgaggg accccgcggc aacgcggacc cggccgccgc
    12001 ggcgcacttc atccgggaga ccttcgcccg gatggcgatg aacgacgagg agaccgtcgc
    12061 gctcatcgcc gggggccaca ccttcggcaa gacccacggc gccggtatcg ccgacgacca
    12121 cgtcggcccg gagcccgaag ccgccccgct cgagacgcag ggcctcggct ggctgagcac
    12181 ccacggcagc ggcaagggcg cggacacgat caccagcggt ctcgaggtga cgtggaccga
    12241 caagcccacg cagtggagca accggttctt cgagatcctc ttcggctacg aatgggagct
    12301 caccacgagc cccggcggcg ccaagcagta cgtcgccaag gacgccgagg cgatcatccc
    12361 ggacgcccac gacccggcca agaagcacaa gccgaccatg ctcaccacgg acctggcgct
    12421 gcgcgtcgac cctgagtacg agaagatctc gcgccgcttc ctggagaacc cggacgactt
    12481 cgcgctcgct ttcgccaagg cctggtacaa gctgctgcac cgcgacatgg gcccggtcag
    12541 ccgcttcctg ggcccgtggg tgcccgaacc gcagctgtgg caggacccgg tgccggacgt
    12601 cgaccacgag ctcgtcgggg acgccgagat cgccgcgctg aagacgaagg tgctcgaatc
    12661 cgggctcacg gtcgagcagc tggtcggcac ggcgtgggcg tccgccgcga gcttccgctc
    12721 caccgacaag cgcggtggcg ccaacggtgc gcggatccgc ctggcgccgc agcgtgactg
    12781 ggaggtcaac cagcccgagc agctcgcgag tgtgctggag accctggaag gcatccagcg
    12841 cgagttcaac gacgccggtg gcgccaagat ctcgctggcc gacctgatcg tgctggcggg
    12901 cacggccgcc gtcgagaagg ccgcgcgcga cgcgggcacc gaggtgaccg tcgcgttcca
    12961 ccccggccgc accgacgcca cccaggagga caccgacgtc gagtcgttcg cgctgctcga
    13021 accgcgtgcc gacggtttcc gcaaccacct gcgtcccgag gagaaactcc agccggaggt
    13081 cctgctcgtc gagcgcgcct acatgctcga cctgaccgcg cccgagatga ccgtcctggt
    13141 cggcggcctg cggtcgctcg ggatcaccgc ccccggcacc cggcacggcg tcctcaccga
    13201 ccggccgggc atgctcacca acgacttctt caccaacctc ctgtcgccgg gcacccggtg
    13261 gaaggtgtcg gagtcggagg agaacgtgta cgagatccgc gacgccgcca cggacgcggt
    13321 gaagtggacg gcgaccccgg tcgacctcgt gttcggctcc aactcgcagc tgaggg
//
