LOCUS       NZ_LT629789            11284 bp    DNA     circular CON 13-MAY-2024
DEFINITION  Amycolatopsis keratiniphila strain FH 1893 chromosome I.
ACCESSION   NZ_LT629789
VERSION     NZ_LT629789.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis keratiniphila
  ORGANISM  Amycolatopsis keratiniphila
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis; Amycolatopsis japonica group.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900105855.1-RS_2024_05_13
            Annotation Date                   :: 05/13/2024 02:01:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,886
            CDSs (total)                      :: 8,813
            Genes (coding)                    :: 8,742
            CDSs (with protein)               :: 8,742
            Genes (RNA)                       :: 73
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 51 of 71
            Pseudo Genes (internal stop)      :: 1 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:07:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 8813532
            Orig. end                         :: 8824816
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT629789.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11284
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="34"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11284
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11284
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6284](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6284
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(<634..1242)
                     /locus_tag="BLU53_RS45935"
                     /pseudo=""
     CDS             complement(<634..1242)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /locus_tag="BLU53_RS45935"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent oxidoreductase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKGKPMKVLVAGGTGFIGQHVVRELLARGHDVTVLVRTSPTNLPD
                     AVTVRLGDLTGLSGERLDTIVAGHQGIIVASAAAVAAPLGTDVAAFLHDANVAPVARLL
                     AAGCRAQTDRAVVLGSFYATLQRERPSLRLAELSPYIASRIEQAERARAAVDAETSVAV
                     LELPYVAGKTPDRQSPLEPMLRSMSVGDADILTYPGTTAV"
     gene            complement(1239..2516)
                     /locus_tag="BLU53_RS45420"
                     /old_locus_tag="SAMN04489733_8315"
     CDS             complement(1239..2516)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030353.1"
                     /locus_tag="BLU53_RS45420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8315"
                     /product="DUF3100 domain-containing protein"
                     /protein_id="WP_063274420.1"
                     /transl_table=11
                     /translation="MSEWSSAKTGTAVVIGVTALCALVTVGTEYAGALELRIGSFALVL
                     LPLFFAFVITVALNLSLSWRRPDAAAPIWSCAGRLLQLLTVVFVVRAGVVLGTALPTIV
                     DAGPAILVQSLGKAGTVLLAFPAALLLGFGRASIGATFSIGREPSIALVTERYGISGPE
                     GSGVLATYISGTLFGSLFFSVLAGIGSTIPLFSSTALAMACGVGSGGMTISCAGSLAHG
                     SRDLPQDDILALATASDVLTYVTTVLLGAFVVLPVLERLYRSPTAVRNRLVSGDTVEPK
                     AVVAPSRLVIGLAVTGLLLVTSGWHAAPGMLLLLALAFGVRMLGDRTSRFVPTVLWAAL
                     AGAVLTVPGLPWSATMLPILSAVDLLTLATAVLSCVGLCVTIRQLRILRTLSWRAVVVT
                     LLVFAGSFFTSALLAQIVLSLGGFTG"
     misc_feature    complement(2409..2411)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(2577..2732)
                     /locus_tag="BLU53_RS44930"
                     /old_locus_tag="SAMN04489733_8316"
     CDS             complement(2577..2732)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLU53_RS44930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04489733_8316"
                     /product="hypothetical protein"
                     /protein_id="WP_157376288.1"
                     /transl_table=11
                     /translation="MVTSAPLRRSAAILATSSGNIGHIPSGPEFITATIVPRTAAGRGV
                     LAAADG"
     gene            complement(2736..3692)
                     /locus_tag="BLU53_RS40560"
                     /old_locus_tag="SAMN04489733_8317"
     CDS             complement(2736..3692)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336687.1"
                     /locus_tag="BLU53_RS40560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8317"
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="WP_063274421.1"
                     /transl_table=11
                     /translation="MLRIHFTDRDLARVRVAAAPDPLWETVLAMQKLTLDGVPAFARWR
                     RRVREGLRERDARTAVRFLQTIAPPARYFPDFLTPAESACGLKAGLAALRGTPADRLER
                     EVGLMAKQHCLPGWTHGLAAGASAALDQVAEALQLAHDTLVAPQWATVRTQVDIDRALR
                     SRLAGESGTLGLLESLRPAFRWEAPVLTVRYPVDRDLHLDGRGIRLVPSYFCWDTPVAL
                     ADDDLPPVLVFPVSHEHEPAPLPKHLATLLGRTRAAVLYAVAGRPTSGELADRLGISAA
                     AVSRHTTVLRDAGLIASRRHGAVVLHNLTPMGAALLG"
     misc_feature    complement(3780..3791)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
     gene            complement(3916..4059)
                     /locus_tag="BLU53_RS44935"
                     /old_locus_tag="SAMN04489733_8318"
     CDS             complement(3916..4059)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLU53_RS44935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04489733_8318"
                     /product="hypothetical protein"
                     /protein_id="WP_157376290.1"
                     /transl_table=11
                     /translation="MAVIRESLLPAVAEWYPGDDEVHDFVAELVDLVKQAQALEPISQN
                     DR"
     gene            complement(4067..4192)
                     /locus_tag="BLU53_RS46115"
     CDS             complement(4067..4192)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLU53_RS46115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_267284182.1"
                     /transl_table=11
                     /translation="MSGDFIAGEVADDKLSEELRHFTEGEYGYFSLSAYTSKQAS"
     misc_feature    complement(4736..4749)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.97"
                     /tool="antismash"
     gene            5001..6284
                     /locus_tag="BLU53_RS40565"
                     /old_locus_tag="SAMN04489733_8319"
     CDS             5001..6284
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR00702.1"
                     /locus_tag="BLU53_RS40565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8319"
                     /product="YcaO-like family protein"
                     /protein_id="WP_228708422.1"
                     /sec_met_domain="YcaO (E-value: 1.7e-48, bitscore: 165.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDRVTSAAAEWTPPPPRSTSGSPARRSQSRPRQAPSVGQSEDRG
                     HSQEFLHRHAPHSAPRAHLGGHAAASEHLRRDEGRRCDRARRDRDPGLYGVRPLARTLS
                     VSQGKGHSLLLAKISAVMESIELWHAEYGCPPPDVRDVPAVDLGLPYRLEQLDNYPGGL
                     VGDRTPLDWLSGRGLVGGSSLLVPHHMVHMARNLTSDWSPLGMRNGSNGLASGNNVPEA
                     TLHAVYEVLERNSLRHLTTSLDGRVNIDPASVEDPACAELIDKILDADAVFEIAMVPNR
                     WGIACFVAYVWSHDFPVFSVGSGAHASVGVALSRAITESVQSRLTAISGTRDDLAPMYA
                     HVQRRSGELPPVPRDLASFAEISSEGGGPEFDDVEAELAWISRRIQDFTGVEPIAVNLS
                     TSEDFAVVKVVAPGLRFADRHVVPRPRY"
     gene            complement(6463..7281)
                     /locus_tag="BLU53_RS40570"
                     /old_locus_tag="SAMN04489733_8320"
     CDS             complement(6463..7281)
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082268.1"
                     /locus_tag="BLU53_RS40570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8320"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_072031344.1"
                     /transl_table=11
                     /translation="MTTAAQEAFLRAFHAHRPAVTSGAMSQGRASDGRSSYEILRDQVE
                     GHPRVLDLGCGDGFLLDLLAGAGHVVTGVDLSGAELLVARQRPSLAAATLLEGRAQALP
                     FTDGAFDACVSHMAFMLMPEVERVAAELARVLMPGGRLALVLGGGGAGGDGAYELFQRL
                     LRPVLADAPAEHRTPRLGDRRTRHREGIDEILTPPGFSPVEWATEQIDLSGSAEQVWST
                     VSVTYNLMPLDPTIVKSIEDEFLSQAAGLRDAGGRVPCTMNVHVVTTTLG"
     gene            complement(7367..7891)
                     /locus_tag="BLU53_RS44940"
     CDS             complement(7367..7891)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLU53_RS44940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_158077409.1"
                     /transl_table=11
                     /translation="MNLMSHRQGRLVEIEVAPAQAKGLATPQPGQGRDMEQGMQRMMPS
                     ELEKRRVQLAPAWRGLGGVGRLLTARLLRWICTDPRLVAVLPFPIDLDRGLLKDDSIFE
                     PALEKVRRTWAALGFQPATDQLWIMDPADGAHESTITSSMHTLALIDRPNSHVSHHRFA
                     LRHSAWTCPAR"
     misc_feature    8347..8362
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.04"
                     /tool="antismash"
     misc_feature    8955..8974
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: weak, score: 22.88"
                     /tool="antismash"
     gene            complement(9011..9478)
                     /locus_tag="BLU53_RS40585"
                     /old_locus_tag="SAMN04489733_8322"
     CDS             complement(9011..9478)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637482.1"
                     /locus_tag="BLU53_RS40585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8322"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_072030493.1"
                     /transl_table=11
                     /translation="MTTQQISDAELAGQPAAYWTGVAYEALIAYTRAQQAEKGYTQPQF
                     WLLRNLSANDISPDGEGMTLPELREVMTSYLRPEDDLKAEAEVLLDRGWLTQDAEDRLW
                     LTKEGEQARVDLARNAPAIRAALHEGIDDADYVTTLKVLQQLIRNTGGIVA"
     misc_feature    9478..9492
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.21"
                     /tool="antismash"
     misc_feature    complement(9567..9581)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.02"
                     /tool="antismash"
     gene            <9691..10218
                     /locus_tag="BLU53_RS45845"
                     /old_locus_tag="SAMN04489733_8323"
                     /pseudo=""
     CDS             <9691..10218
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689697.1"
                     /locus_tag="BLU53_RS45845"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="SAMN04489733_8323"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="VTMEGRRDVLGLRAGQGVEGAKFWMQVLIEMKNRDLGECVACDDR
                     ADVRGSGSRRSVRRSRWMSWRTGGA"
ORIGIN
        1 cttcaccgcg cgggcgacga ccccggcgct ctgctggtga cggcgggttg tgccttgctg
       61 gtcagcccga tctcgtggac gcaccactgg gtatgggtgg tgcccgccct cggctacctg
      121 tgcgccaggc ggcccgcgat gggtatggcc gccgtcgtgt tcacgggctg gaccgtcgcc
      181 gtggttcccg gtggcggcgg cgccgaacgc acgtggaacc tcggacaagc cgtcgtcggc
      241 aatgcctatc cgatcgcggc gctcgctttg ctgttcgtgc tcggccgccg ctcatcggag
      301 ggcttcgccg aggtcgtccg gctcgacgcc gagtccggtg cgggcgatgt ccgggtcgag
      361 gaccatcgcg gtggagtgca ggtcgctcca ttgcgccggg tggaaaccga gggtgaatcc
      421 ttggcggcgt tgacgaatcc tcgcccagcg gagcagaagc tcgatggcgc ggggatgaag
      481 tttccaaatg cgtttcggcg ggcggccggc cgcggtggcg aggcgagaga agagatcggc
      541 ccacgggaga ttcgtcgtga cgaccggata gttcccccgc gcccggcgtt ccaacgccgc
      601 gacagccgct cgcgcggcct gggtgacggt gacgaccgcg gtggtgccgg ggtaggtgag
      661 gatatccgcg tcgccgacgg acatgctgcg cagcatgggt tcgagaggtg attgccggtc
      721 cggggtcttg cccgccacgt aaggaagttc gagaaccgcc acagaggttt ccgcgtcgac
      781 ggcggcgcgg gcgcgctcgg cttgttcgat cctgctggcg atgtacgggc tgagctcggc
      841 cagccggagt gacggtcgtt cgcgttgcag ggtggcgtag aagctgccga ggacgacggc
      901 gcggtcggtc tgcgcccggc agccggcggc gagcaacctg gcgacggggg cgacgttggc
      961 gtcgtggagg aacgcggcga cgtcggtgcc gagcggtgcg gcgacggcgg cggccgatgc
     1021 gacgatgatg ccctggtggc cggcgacgat cgtgtcgagc cgttccccgg acaggccggt
     1081 gaggtcccca agccggactg ttaccgcgtc gggaaggttc gtgggcgacg tgcggaccag
     1141 cacggtgacg tcgtgcccgc gcgccaggag ctcacgcacg acgtgctgtc cgatgaagcc
     1201 ggttccgcct gctaccagga ccttcatggg ctttcctttc atccggtgaa tccgccgagg
     1261 gacaggacga tctgggccag cagggccgag gtgaagaagg agccggcgaa aacgagcagg
     1321 gtgacgacga ccgcgcgcca gctcagcgtc cgcaagatcc tcagctgccg gatggtcacg
     1381 cacagtccga cgcagctgag cacggccgtg gccagcgtca gcaggtcgac agcggacagg
     1441 atgggcagca tggtcgcgga ccaaggaagt cccggaacgg tcagcaccgc gccggcgagc
     1501 gccgcccaca gcacggtcgg cacgaaccgg ctggtccggt cgccgagcat ccgcaccccg
     1561 aaggcgaggg ccagcagcaa gagcattccc ggcgccgcgt gccatcccga ggtgacgaga
     1621 agaagtccgg tcacggccag gccgatcacc agccgagagg gggcgacgac cgccttcggc
     1681 tcgaccgtgt ccccggatac cagccgattc cgcaccgcgg tgggggaacg gtacagccgc
     1741 tccagcaccg gaaggacgac gaaagcgccg agcagcacgg tggtgacgta ggtcagcacg
     1801 tcgctggccg tcgccagcgc gaggatgtcg tcctggggca ggtcacggga accgtgcgcg
     1861 agtgagcccg cgcaggagat cgtcatgccg ccgctgccga cgccgcaggc catcgccagc
     1921 gccgtggaac tgaacagcgg aatcgtcgac ccgatgcccg cgagcacgga gaagaacaag
     1981 gacccgaaga gcgtgccgct gatgtaggtg gcgagcacgc cggaaccttc cggtccggag
     2041 atgccgtacc gctcggtcac cagggcgatg ctgggttcgc ggccgatcga gaaggtggcg
     2101 ccgatactcg cgcgtccgaa gccgagcagc agggccgccg ggaaggccag cagcacggtc
     2161 cccgcctttc cgaggctctg cacgaggatc gccggcccgg cgtcgacgat cgtgggaagc
     2221 gccgtcccca gcacgacccc ggcccgcacc acgaagacca ccgtcagcag ttgcaggagt
     2281 cgcccggcgc aggaccagat cggcgcggcc gcgtccggac gccgccagga caacgacaga
     2341 ttgagggcca ccgtgatcac gaaggcgaag aaaagcggaa gcaggacgag cgcgaaagag
     2401 ccgatccgta attccagcgc gcccgcgtac tcggttccca ccgtgaccag cgcgcacaac
     2461 gcggtgaccc cgatgacgac cgccgtcccg gtcttggcgc tcgaccactc ggacatgacc
     2521 accccccgga acccaccatg cccgattccc ggcgggcgtg gtcggagcaa gcgtcgtcag
     2581 ccgtccgcgg cggccaggac gccgcgcccg gcggcggtgc gcggcacgat cgtcgcggtg
     2641 atgaactccg gcccggacgg gatgtggccg atgttcccgc tactcgtggc gaggatcgcc
     2701 gcggatcgcc gaagtggtgc ggaggtgacc atggctcagc ccagcaacgc ggcacccatc
     2761 ggcgtgagat tgtgcagcac gacggctccg tggcgacggc tggcgatgag cccggcgtcg
     2821 cggagcaccg tggtgtgccg cgaaaccgcg gccgccgaga tgccgagccg gtcggcgagt
     2881 tcgccgctgg tggggcggcc cgcgaccgcg tacaggacgg cggcgcgggt ccggccgagc
     2941 agggtggcca ggtgtttcgg caggggagcg ggctcgtgct cgtgggacac cgggaagacg
     3001 aggaccggtg gaaggtcgtc gtcggccagg gcgacggggg tgtcccagca gaagtaggac
     3061 ggtacgagcc ggataccgcg accgtcgagg tggaggtcgc ggtcgacggg gtagcgcacg
     3121 gtcaggaccg gcgcctccca gcggaaggcg ggccggagcg actcgagcag gccgagggtg
     3181 ccactttcgc cggcgaggcg tgaccgcaag gcacggtcga tgtcgacctg ggtccgcacg
     3241 gtcgcccatt gtggagcgac gagggtgtcg tgggcgagct ggagcgcttc ggcgacctga
     3301 tcgagcgccg cgctcgcacc cgccgccagc ccgtgtgtcc aaccgggcag acagtgttgc
     3361 ttcgccataa gcccgacttc gcgctcgagc cgatccgccg gcgtcccacg aagcgccgcc
     3421 agccccgcct tcagcccgca cgcggactcg gcgggggtga ggaagtccgg gaagtaccgg
     3481 gcaggcggcg cgatcgtctg caggaaccgc accgcggtcc gggcatcacg ctcccgcagg
     3541 ccttcccgca cccgccgccg ccagcgcgcg aaggcgggaa cgccgtcgag cgtgagcttc
     3601 tgcatggcca gaaccgtttc ccacagcgga tccggtgcgg ccgcgacgcg gacacgggcc
     3661 aggtcacggt cggtgaagtg aatacgaagc atgacgcctc ccccatggag tcgtcattca
     3721 tttatcacat ctgaggggac catgaagggg attccgggca gaaacctgcg gcggcactcg
     3781 cgttcacgtg cctgaagtac aaccgctggg tcgcagaccg aaccggccgc acatgtgaac
     3841 aaagcgcacc cacggcatcc gagatgaacc gttgctgggc ttcgttccgg aaccatcgat
     3901 tcggggcatc gccgctcatc ggtcgttttg gctgatcggc tcaagcgctt gggcctgctt
     3961 cacgagatcg accagctccg ctacgaagtc gtggacttcg tcgtcgcccg ggtaccattc
     4021 ggcgaccgcg ggcaggagcg attcccggat aacggccatg atctcttcag cttgcttgct
     4081 tgcttgtgta tgcgctcaga gaaaagtagc cgtactcacc ttcggtgaag tggcgcagct
     4141 cttcggaaag cttgtcgtcc gcaacctcgc cggcgatgaa atctccggac aactcatttc
     4201 accttaggaa ctgcgcattg atcaggttcg tcgtcggcga aggtcgcttc ggcgtcgccc
     4261 gtcggcctcc cctgttcggt ccgtgatcaa ctcccggtat cgagggtcgt ctcgggctgt
     4321 gacggcgttg tcgtgagttc cgcccgccgg gcccatgatc cacaatcttt tcgtctggtc
     4381 ggaggctgtc cgtagtgtcg tggtgtgtcg cctggtgtcg gactgtttcg gccctctcgg
     4441 gcgcgtctcg ggctcgtgtg tgccgaatcg tgtcacgtcg tccagcctca atcgcgatcg
     4501 cccggaccgc gagctgtcgg catcgggacg tccagacgcg agacgccgaa ctgccgtgag
     4561 atgctgagag agcggacgtc gtgcgcgaag cggtgcggaa cctgtccctg aagtcgcgtt
     4621 tacccggcct ccggccagca tccggtcact gtggcggtca gctgccctga gtggtcactg
     4681 cgatatggct gtgcatgctc cataaccgag accggatctg ctcccgtgga taaggtgaat
     4741 gcgcatgcgg gaggggtggg atggaggcag ttcggcgtgg tgacaggtgg acagaggagg
     4801 aagaagatca ccttctgtgc aagctgttcg atcaggaaga acccgaggcg atcgcggctg
     4861 cacatcagcg ctcggtggtt gcgatcctgt tgcgggtcga gcagttggtc gacggcccgg
     4921 ctgatcttga ggacaatcta gctgttttcg aatgggccag aagtgaactt ctcaaaggac
     4981 aggcaggcag cctggcctcc atgctcgaca gggtcacttc ggcagccgct gagtggacac
     5041 cacctccgcc acgttccacg tccgggagcc ctgcgcgacg atcgcaatcg cggccacggc
     5101 aagcaccttc ggtgggacag tcggaagacc gcgggcactc gcaagagttt cttcaccggc
     5161 acgcaccgca ctcggctccc cgagcacacc tgggaggtca tgcggccgca tctgagcacc
     5221 tacggcgtga cgagggtcgc cgatgtgacc gggctagacg tgatcgggat cccggtctgt
     5281 atggcgtccg gccgttggcg agaaccttga gcgtgtcaca gggcaaaggg cattctctgc
     5341 tgttggcaaa gatctcagcg gtgatggagt ccatcgagct gtggcacgcc gagtacggtt
     5401 gcccgcctcc cgacgtccgc gacgtcccgg cggtggactt gggcctgccg taccggctgg
     5461 agcagctgga caactacccg ggaggtctgg tcggcgaccg gacgccgctg gattggctgt
     5521 cgggtcgtgg cctggtcggc gggagctccc tgctggtgcc gcaccacatg gtgcacatgg
     5581 cccggaatct gacctcggac tggtcaccgc tcggcatgcg caacgggagt aacgggctgg
     5641 cctcgggcaa caacgtgccc gaggccaccc tgcacgcggt ctacgaagtg ctcgaacgca
     5701 actcattgcg acatctgacc acttcgctcg acggccgggt caatatcgac ccggccagtg
     5761 tggaagatcc tgcctgtgcc gaactgatcg acaagatcct cgatgcggac gccgtgttcg
     5821 agatcgccat ggtgccgaac agatggggca tcgcatgctt cgtcgcgtac gtgtggagtc
     5881 acgacttccc ggtcttttcg gtcggttcgg gcgcacacgc ctcggtcggg gttgccctga
     5941 gccgggccat cacggaatcc gtccaaagca ggctcaccgc gatcagcggc acccgtgatg
     6001 atctcgcccc gatgtacgcg cacgtccagc gtcgctccgg cgagctgccg ccggttcctc
     6061 gagacctcgc ctccttcgcg gagatcagct cggaaggcgg tggtccggag ttcgacgatg
     6121 tcgaggcgga actcgcgtgg attagccgaa ggatccagga cttcacagga gtggagccga
     6181 tcgccgtgaa cctgagcacc tcggaggact tcgcggtcgt caaggtcgtc gcgccagggc
     6241 tcaggttcgc cgaccggcat gtggtgccga gaccgaggta ctgactccag gtggacgttc
     6301 ccaaggatct gcgggacctc atgattcgga aggcacccgc gcacagacac cggcagcgga
     6361 accggtgaaa tgggtctgtt tccagcactc cggagtgctg gaaacagacc ttccggcaag
     6421 agcagggcga gaacgtcgtg ttcgtgcggt cggcgagccg gatcagccaa gtgtcgtggt
     6481 gaccacgtgg acgttcatgg tgcaggggac ccggccaccg gcgtcgcgca gcccggctgc
     6541 ctgggacagg aattcgtcct cgatcgactt cacgatcgtg gggtccaggg gcatcaggtt
     6601 gtaggtcacc gagacggtcg accacacctg ctcggcgctg ccgctgagat ctatctgctc
     6661 cgtagcccac tcgacaggcg aaaatccggg cggagtgagt atctcgtcga taccttcgcg
     6721 gtgccgggtg cgccggtcgc ccagtcgcgg agtccggtgt tcggcggggg cgtcggccag
     6781 cacgggccgg agcagccgct ggaacagttc ataagcgccg tccccgccgg ctccgccgcc
     6841 gccgagcacc aaagccagtc gtcctccggg catcaggaca cgggccagtt ccgcggcgac
     6901 gcgttcgacc tccggcatca gcatgaacgc catatgtgac acgcacgcat caaaggcgcc
     6961 gtcagtgaag ggcagcgcct gggctcgtcc ctcgagcagc gtcgcagcgg cgagggatgg
     7021 gcgctgccgg gcgaccagca gttcggcgcc cgagaggtcc actccggtga cgacatgccc
     7081 ggccccggcg agcaagtcga gcagaaagcc gtcaccgcaa ccgaggtcga gcactcgtgg
     7141 atggccctcg acctggtctc gcagtatctc gtagctcgat cggccgtccg acgcgcggcc
     7201 ctggctcatc gcgccagagg taaccgcggg gcgatgtgcg tggaacgcac gtaggaaggc
     7261 ttcttgagct gccgttgtca tgatcactac attaggctgg ttggacgggg caggcggttg
     7321 atccgccgag gcccatggga gctggtcaat gccgacaaaa gtgtcgtcac cgtgccggac
     7381 atgtccatgc gctgtgtcgc aaagcgaagc gatggtgcga tacgtgtgag ttcggtctat
     7441 cgatcaatgc caaggtgtgc atcgagctgg tgatggtgct ctcgtgtgct ccgtccgcag
     7501 ggtccatgat ccacagctga tcggtggcgg gttggaatcc gagtgctgcc caggtgcggc
     7561 gtactttttc gagtgccggt tcgaagatcg agtcgtcttt gagcaggccg cggtcgaggt
     7621 cgatggggaa cggcagtaca gcgacgagtc gtgggtcagt gcagatccag cggagcagcc
     7681 gggcggtgag tagtcgtccg actccaccga ggccgcgcca ggccggtgcg agctgcacgc
     7741 ggcgtttctc cagttcgctg ggcatcatcc gctgcatccc ttgttccatg tcgcggcctt
     7801 gaccgggttg cggcgtggcg aggcccttgg cctgcgctgg tgcgacctcg atctcgacga
     7861 ggcgaccttg gcggtgactc atcagattca agagcgggac tgcaaagcgg tagtgtgcct
     7921 gccgaagacc gcggcccagg cggaggcgct ccggacggtt gggtcttcgc gcacgggaat
     7981 gggcagtatt ggagcccaag ttacgtatcg cattcgttcc gtcggctgat catcaagtct
     8041 gggctgccgc cggtacgttt ccacgacctc tgtcatggcg cggcgacgct gtcgctggcc
     8101 gcggggaacg acctcaagac ggtgcaagcg ctgctgggcc acgcaagcat cgtcctgacg
     8161 gccgacacct acaccagcgt actcccgtcc ctcgcccatg ccagcgccga agcgaccgcg
     8221 agcctggttc gtcgagccgg ctacgaacgc ggtcagaaga tctgcaggtc gcgcgccact
     8281 cgtggtcgac aacaacgaag ccaggaccgc ggccgaggtc cccgcctcat tcatcatgga
     8341 gggacatttg acggggctca cgatggcccc acctcgggct cacacatcaa caaagcccac
     8401 cgacgacacc cttgaaatca agggataatc gttggtgggc tttggtttct tactgtgcgc
     8461 catcagggac tcgaaccccg aacccgctgg ttaagagtcg gtcctctagg gctcgcggac
     8521 gccggattgc cgcttggccc agccgccttg aagggttgcg ttgccgtgcg gtgcgaccac
     8581 tggcctgcgc ggtggtcgtg ggggcggtca tcacgtgcac ggtcgcctcg gtcgtcggct
     8641 tgcgcccagg gacgtccggg agatccacga cacggcgcga ccggtggcgc gcaaccggtc
     8701 gagaagttgc tgcccgttgc ggcggggcgt gtcgcggatc ttcccgtcgg acacgatgat
     8761 cagcaaccgc gcggcaccgg gggctcgaca gtccgagcgc gcatccgaag gcgaccgggc
     8821 ggaatcggcg aggtcgtctt cgtcgccacc cggtcacgca ccccggcggt ggtcaacgcg
     8881 cgcccgaaca cccgcgcggc ggtgcgatcg cccgtggtcg gcgggcgcgt gccgatcgtt
     8941 tcggtacgcc cgtcgcgggg accaccgacc gtgaacacgg tgcgggcggc gtcatcgacc
     9001 cctcggtcta tcaggcgacg atcccgccag tgttgcggat cagttgctgg agcaccttca
     9061 gtgtggtgac gtagtccgcg tcgtcgatgc cctcgtggag ggcggcacgg atcgcggggg
     9121 cgttgcgtgc gaggtcgacg cgggcctgtt ccccctcctt ggtgagccac agccggtcct
     9181 cggcgtcctg ggtcagccag cctcgatcca ggagcacctc ggcctccgcc ttcaaatcgt
     9241 cctcgggccg gagataggag gtcatgacct ctcgaagttc cggcagggtc atcccctcgc
     9301 cgtcaggtga gatgtcgttc gccgacaggt tgcgcagcag ccagaactgc ggctgggtgt
     9361 atcccttttc ggcctgctgg gcccgggtgt atgcgatgag cgcctcgtag gcgacaccgg
     9421 tccagtacgc ggcgggctgt ccggcgagct cagcgtcgga gatctgctgg gtcgtcatgg
     9481 atgtcctttc ctggtaggcc tccgtaagac ctgcaggcag tgcagatcat acggagaccg
     9541 tagaacctca agtaaagttg aagtcaagca agtgatgttc aggacgattc gccgtccgcg
     9601 gtgaacgccc agccgggttg cgtgcagttt aggcacaaga cgtattcgtt ccgctacgac
     9661 ggctgaccgt cgttaatcag ctgggccgat gtcacgatgg aaggtcgtcg ggatgtgctc
     9721 gggctgcggg ccggtcaggg cgtcgagggt gcgaagttct ggatgcaagt gttgattgaa
     9781 atgaaaaacc gtgacctggg cgaatgcgtg gcctgcgacg atcgcgcaga cgtgcgtggt
     9841 tccggctcgc gtcgaagcgt gcggcggtcg cgttggatga gctggaggac tggtggggcg
     9901 taagtatggc gcgatgatcc gtttgtggcg caacacatgg gaggagttca tcccgttttt
     9961 ggactatgct gcggagattc gtcgcgtgat gtgttccaag agtgcgattg aatccttgaa
    10021 ttcaaggtat cgtcgcgcgg tgcgggcacg tagccatttc ccgacggggc aagctgcgtt
    10081 aaagtgcttg tatctggtca cccggagtct ggactcgacc gccaccgggc aagcccggtg
    10141 gaagatgcga tggaacccgc actaaacgct ttttcgatca ccttcagtga ccgctggccg
    10201 ggcgctgaga cctactgata accacggccg gaaacaccgt tcgcgttaca gtcccgacgg
    10261 ctgggcggcg gatccctggt gggtcattcc ggtgtggcgc ggacaagcgt cttgccggtg
    10321 ttttcgccgc gtagcatacc gaggaacgcg tcgacgatgg tgtcgaagcc gtcggtcacg
    10381 gtctcgtcga ccttgatccc gccgcgccgc acgtgcggga tcaggaactg ttcgaactcc
    10441 tcacgcgcgt ccatgtggtc gcgcacgagg aaaccttcca accggatgct tttgccgacg
    10501 atgtcgaaga ggttgtacgg cgcggcgggc ggattatcca ggctgttgta ctgggcgacc
    10561 gcaccgcacc aggcgatcct cccgtgatcg cgcagcgcgt cgatagcagc cgcgaggtgg
    10621 ttgcctccga cgttgtcgaa gtagacgtcg atgccttccg gcgccgctgt cgcgagtgag
    10681 tcggggatcg ggccagcgtg gtagtcgaac gcggtgtcga agccgagccg cgtggtcagg
    10741 tgctcgacct tggccgcaga ccccgcgcta ccgacgatcc ggcccgcgtg cagcagtcgc
    10801 gcgatttgtc ctgtcgcgct gccgacggcg ccggccgcgg ccgatatgaa caggtcctcg
    10861 ccgggctgca accgggcgat gcgggtgagg ccgacgtacg cggtcagccc ggttccgccg
    10921 aggatgccga ggaaagcgct gagcggaacg ccgtccactg gggtgaccac ccgaacgtcg
    10981 gcctcggcga gcgcggcgtg cgtgcaccag ccgtgccggt ggaagacgat ggttcccacc
    11041 gggagagccg tcacccttga ctcgatcacc cgccccagcg tgcggccttc gagcccggct
    11101 cccttttccc agccgccaaa gtccatcagc tcccgcatgt acgggtcgac ggacagatag
    11161 atgttttcga cgaggacctg tccatcgccg ggaccggcca actgttcggt gatgaaggcg
    11221 aagtcctccc tgcgcggctc gccctgcgga cgacggactt ggcggacggc gctgacactg
    11281 cggg
//
