LOCUS       NZ_LT629789            10390 bp    DNA     circular CON 13-MAY-2024
DEFINITION  Amycolatopsis keratiniphila strain FH 1893 chromosome I.
ACCESSION   NZ_LT629789
VERSION     NZ_LT629789.1
KEYWORDS    RefSeq.
SOURCE      Amycolatopsis keratiniphila
  ORGANISM  Amycolatopsis keratiniphila
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Amycolatopsis; Amycolatopsis japonica group.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900105855.1-RS_2024_05_13
            Annotation Date                   :: 05/13/2024 02:01:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,886
            CDSs (total)                      :: 8,813
            Genes (coding)                    :: 8,742
            CDSs (with protein)               :: 8,742
            Genes (RNA)                       :: 73
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 26 of 71
            Pseudo Genes (incomplete)         :: 51 of 71
            Pseudo Genes (internal stop)      :: 1 of 71
            Pseudo Genes (multiple problems)  :: 6 of 71
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:07:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7364337
            Orig. end                         :: 7374727
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT629789.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10390
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="28"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10390
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10390
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5390](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5390
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(235..249)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.74"
                     /tool="antismash"
     gene            3187..3678
                     /gene="ectA"
                     /locus_tag="BLU53_RS33675"
                     /old_locus_tag="SAMN04489733_6892"
     CDS             3187..3678
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033456.1"
                     /locus_tag="BLU53_RS33675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_6892"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_063273065.1"
                     /transl_table=11
                     /translation="MSGKHLIETPTKADGAALWRIARDSKKLDLNTPYAYLLWCHDFAD
                     TSVVAKVDGVPVGFVIGYRKQDTGFVWQVAVDASQQGKGLAGALLDELFGRLTAQGVRY
                     LETTITPDNEASIRLFASFAKRWDTTVERQDLFSATDFPAEEGTHEQEDLYRIGPLSSQ
                     "
     gene            3717..4967
                     /gene="ectB"
                     /locus_tag="BLU53_RS33680"
                     /old_locus_tag="SAMN04489733_6893"
     CDS             3717..4967
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 4.8e-73.
                     Score: 238.0. Matches aSDomain:
                     nrpspksdomains_BLU53_RS33680_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631928.1"
                     /locus_tag="BLU53_RS33680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_6893"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_063273066.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1e-74, bitscore:
                     250.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIFEELESEVRSYSRGWPVVFDRAQGSYLYDEDGKAYLDFFAGA
                     GALNYGHNNPALKRALIDYIARDGVTHALDMFTVAKRDFLQTFQEKILQPRDLDYKVVF
                     PGPGGANAVEAALKLARKVTGKESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHA
                     TPMPYDNYFDGSIPDFLYFEKLLEDSGSGLNEPAAVIVEGVQGEGGINAARIEWLKALD
                     DLCKKHGILLILDDVQMGCGRTGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPD
                     LDVWEPGEHNGTFRGISPAFVTATEAIRVYWSDDELEKSTKAKGERIAAAFQGIVEAYP
                     DANLFAKGRGLARGIEFANGDLAGKVCAAAFERGLLMETSGPDGEVMKVLPPLTLTDDE
                     LTKGLSIIDEAVATVLN"
     aSDomain        3780..4790
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLU53_RS33680_Aminotran_3.1"
                     /evalue="4.80E-73"
                     /label="BLU53_RS33680_Aminotran_3.1"
                     /locus_tag="BLU53_RS33680"
                     /protein_end="358"
                     /protein_start="21"
                     /score="238.0"
                     /tool="antismash"
                     /translation="FDRAQGSYLYDEDGKAYLDFFAGAGALNYGHNNPALKRALIDYIA
                     RDGVTHALDMFTVAKRDFLQTFQEKILQPRDLDYKVVFPGPGGANAVEAALKLARKVTG
                     KESVINFTNAFHGMTLGALSVTGNSMKRGGAGVPLVHATPMPYDNYFDGSIPDFLYFEK
                     LLEDSGSGLNEPAAVIVEGVQGEGGINAARIEWLKALDDLCKKHGILLILDDVQMGCGR
                     TGPFFSFEDAGIKPDIVCLSKSIGGYGIPMALTLIRPDLDVWEPGEHNGTFRGISPAFV
                     TATEAIRVYWSDDELEKSTKAKGERIAAAFQGIVEAYPDANLFAKGRGLARGIEFA"
     gene            5001..5390
                     /locus_tag="BLU53_RS33685"
                     /old_locus_tag="SAMN04489733_6894"
     CDS             5001..5390
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165143.1"
                     /locus_tag="BLU53_RS33685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_6894"
                     /product="ectoine synthase"
                     /protein_id="WP_007033454.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.2e-53, bitscore:
                     179.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVRTLDEVTDTDADIKTPNWRSKRIVLAKEGVGFSVHETTLYAG
                     TVNDFWYANHIEAVFITSGEGELENTATGEVFQLKPGTLYLLNDHDKHQVRPKTEIKCV
                     CVFNPPITGREVHDENGVYPLVTEP"
     gene            5415..6314
                     /gene="thpD"
                     /locus_tag="BLU53_RS33690"
                     /old_locus_tag="SAMN04489733_6895"
     CDS             5415..6314
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /locus_tag="BLU53_RS33690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_6895"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_063273067.1"
                     /sec_met_domain="PhyH (E-value: 3.9e-31, bitscore: 108.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLTDTRVDDSYPTRITGKPEHLPRTHPTVWGTEADGPLDAATLA
                     NHETRGYTVVDQLLSPGEVQTYWQELVRMSSGDHSGDERVITEAKTGEVRSIFDVHETS
                     ELIAELVRDPRVLDRARQILGSEVYIHQSRVNYMPGFKGTGFYWHSDFETWHAEDGMPV
                     PRAVSCSIALTDNYPFNGGLMVMPGSQRTFVQCAGETPEDNYKSSLKEQRVGVPSEEDI
                     TALAAEYGIDQFTGQAGSALWFDSNVMHGSSNNITPYPRSNIFVVFNSVENALQEPYAA
                     IEPRPAFIAGRDSTPLTR"
     misc_feature    6650..6661
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    6715..6730
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.48"
                     /tool="antismash"
     gene            6936..9725
                     /locus_tag="BLU53_RS33695"
                     /old_locus_tag="SAMN04489733_6896"
     CDS             6936..9725
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631926.1"
                     /locus_tag="BLU53_RS33695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04489733_6896"
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_072029725.1"
                     /transl_table=11
                     /translation="MAESGSPARFQLLGPVRLLDGDRPVPVGGPGVRGLLALLALKVNK
                     VVALDEIIDALWGHDPPATARTIVHGNVSHLRRVLRDIQGDHPRGARILTAPPGYQLTV
                     EPDRIDVHRARSLLERASTEEPDKASELLAEALSLWQGPALAGVPGSVRAPELEDLRLA
                     VHGARVDADLELGRHAELIVELSPLIRANPLAERTAGQLMRALYHAGRRGDALELYRTV
                     SRATLRTLGVEPGADLRWLHERVLNDDLPVKVIAEPKSETAPVQQLPPAVPNLAGREAD
                     LAWLDELAERAEAGETAVGVVTGTAGIGKSSLVVWWAHRAARRFPDGILFAALRGFDPQ
                     HPPLEPAELLTQFLLGLGVPAEGVPEQVHERVALYRSMIAGRRMLVILDNARSAEQVRP
                     LLPPGSRSMALVTSRSRLDGLAVSNAAKLRVLGTLAPGDAVRLIEELAGPAGYDLNHAL
                     ARLCGYLPLALRIAGARLAASAQWSAQDLVDELGNERTRLAALDVEGPDDGVRAAFDVS
                     FRGLPSEVADTFLRLGVVQCVSAGSHLTAAIGGITVAEARRHLRVLAAHNLLAETGRDS
                     FVPHDLVRLFLRELAENELDEADREAVLSRSVRFYQAVGDRARRKMLRIVDPLDFTGVL
                     TDAQTPPIGSFDDAQDWFTSEWENLLEVLEAARAAGRHDDVWRLARVAHTYRAVYPLLD
                     EWTRLVGIGLEAAERSGDVLGRCWMLISRCAIALTFELPQGCLADAERALELASAIGDS
                     RLMVSANIHLGSALTLLGWYDEAIGTLRQAVEETERTGDLELRGQALNNCAEAEKRAGR
                     FIEAIGHQVASLEIDRTLGDDSYVVVSLNNLAELSMRIGELAAAERYVWEAVDLTISRR
                     FVLQEGVLRLTLGRVLRAGSDVDGAREQFALALKILADANPKLAALVRAELTDLGESP"
     misc_feature    complement(10144..10162)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.56"
                     /tool="antismash"
     misc_feature    10243..10258
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.98"
                     /tool="antismash"
     misc_feature    10260..10275
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: strong, score: 24.95"
                     /tool="antismash"
     misc_feature    complement(10357..10370)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.99"
                     /tool="antismash"
ORIGIN
        1 cggggagccc gtggccgcag gagatccggc tggcgatgac catggtgggc ggggcgagtc
       61 tcgcggtctg gatgggcggg gtcgccaccg agacctcgca tctgttgcag gcgtcgagaa
      121 caccggaatc gaagagcccg taccgggcct tgctcgacct gctcaacgcc accgtctcgc
      181 tcgacgtgct caccggcacg agcgcgggag gtatcaacgc ggcgtgcctc gggctggccg
      241 aggccttccg gtcgagcccg caggtgctgc gcgacacgtg gatcagcacg ggatcgctcg
      301 acaacctgat ccgcgacccc ggtgagaagg aaccgcgttc gctgctcgac ggcgacaagg
      361 tgctgctcgg cgatctcaag gacgcgttgc accggatcac cgacaaggcg acggtgaaac
      421 cggactgtcc cgacatcacc gtgctgctca ccggcacgat gatcgacggc gagacgaccc
      481 gcttcgacga cgcgctcgga aacctggtgc gggacaccga acatcgactg ctgttccgct
      541 ttgacgctcc tttgtggaca gatgacgtcg tcggcccgct cgcgctcgcc gcgcggtcca
      601 ccgcgtcgtt ccccggtgcg ttcgagctct cccggatgcc gatcggcgag aagaccggcc
      661 cgctgcatcc ggacatgacg aagtacaccg acgtcacgcg gtcgcattgg ctcaccgacg
      721 gcggtgtcct gctcaacaag ccgctgcggc cggcgctgcg ggagatcttc gaacggcagt
      781 cgcattcgga cgtccggcgg ctgctgctct acgtggtgcc gacggccgaa cgcgaggccg
      841 agcgggtcgc ggtcgatccc gaacgaccgc cgctgctggg cagcgcgatg agcaaggtcg
      901 tcggcaccgt gctgagccag acgatcagcg ccgaactgga agacctgacc aggcacaacg
      961 acgccgtcgt ccgtaccagg gggacccggg tctcccttgc cgcgatgggg gtgcgcggcg
     1021 ggccggacac gctggtcgac cagcggctga tgaacgacta ccgcgaccgc cgggtgcagg
     1081 aggacgccac cgcgctggtc cgcgaggcga cccgcaggct ttcactgtcc gatgtggagg
     1141 atcaaggccg ccagtgggcg tccggcacgg cggcgcagct gcgcgcggcc gcggccgccg
     1201 ggctgcgcga cggcctgccc accgagccgc cgaaggacac ctgcgacctc gcggacctgg
     1261 tcgccttccg caccaccgcg ctcgacgatt cggtggccac cggcctccag ctggtgaacg
     1321 cgggcttccg gctcgatccg tcgccggcgc aggcggtgca gctcaaccgt tgccgggtgc
     1381 tgctgcacga agcgcgtcac aaggccgcac gcgggaaacg gctcgcgggc tgggtcaccg
     1441 aacagggccc gccgtcggcc gacgtcacgc tggccgcctg gatcgaaggc ctcgccaagg
     1501 aatgggccaa gctcggccgg tcggacacgc tgaaggaggc gtggccgctc gtcgtcgccg
     1561 cgctccggca ggcgacaccg atccttttgc cgctggcaca ggcgaaaccc gataccgaag
     1621 ccgccgacac ggtcagcacg ctgctggcct ggacaggact cacatcgggc gacacgagcg
     1681 ccggagatcc catcgtcacc tcgcggctgg tgcgtctcca catcgcgacc cggggactgc
     1741 tcgcgcagcc gccctcggtc gaccagcggg tcgatctcgt ccaggtgagc gcggattcgc
     1801 ggacgctgat ggacatgaag cgccgccgtt cctgggacaa gctcaccggg atgcaggccg
     1861 actacttcgg cgcgttctac aaggcgtcct ggcgcgcgaa cgactggatg tggggccgcg
     1921 tcgacggcgc gggctggctg gtgcagtgcc tgctcgaccc gaaacgcctg cggctgctgc
     1981 gggacgtcgt cggacgcgag gcgttccggg cgcaggtgcg ggacaccttc aaggggatcg
     2041 gctggcggca gccgggcacg gaagacggtc tgtcccaaga ggaagcggac gccctgcgcg
     2101 cgcaattggc cggggagctg gctttcctcg gtctcgacgg cgaactcgcc gatgtcgaag
     2161 gggaagccgc cctgccgatc agcatgccgg tgacggcgat ggtgctcgcc agggtgcggc
     2221 agctggagat cgcgcgcgag gaactgccct gtgtcggcct ccattgcgga cacgacgcga
     2281 aaacggccaa ggggaacggg aaaccgtcgg aacggttccg ccggatgatc gagaacgagc
     2341 cggagaccga cgagcagacc cagcgcgcgt tccaggcgtg ccaggtctcc ggcgaacggt
     2401 tcgagcacga acgcgggacg atgctcctga cgaagacgct ggtcaaggca ggcgccgccg
     2461 ggctcaacgc agccgcgggg gcgaccaggg tgccgaaatc ggtccagccc gccgcgacct
     2521 tcgccaaggc cgccgggcgc agcgcgtggt ggatcacgcg gggcgcggcc accctgccgt
     2581 caccgtggaa cgtgctcgcc gcgctgatca ccgtgctggc gggcttcgtc atcggtggac
     2641 agggcggccc ggtcctgcag tgggtcggcg tgccggtcgc cgcgggcgcc gtcgtgttcc
     2701 tggtggtcag cctgatgacg ttgcggaaga cgtggcggat ggtgctcacg gtgctcggcg
     2761 tgctggtggt gacggccctg ctgttcgccg cgttcctgcc accggtgcgt gatccgctct
     2821 tcggctggct cggcggcgtc gtcgcgggct ggcggcgcgg cgaggcaccg gtgtggtggc
     2881 tggtcgtctg cctgctgctc gtcctgcccg ccatctggac gccgatcggt tcactcgccg
     2941 gtcgcaagcg ccgtcgtgaa tagcggaatc ggaacccgaa tcgcgtcctg accgtgcgac
     3001 gaagtggtaa cggttggtat cgtcacgtgc agtgagaccg caccgaagcg gccttaccca
     3061 cacgtgctgc gcttcaaacg ttacagttac ctttctcacg tgtgtttcga ggttgttgca
     3121 ggtagccgcg ttgccgctgc cgcgcggatc tgaccgcgcc cgaatcctcg ggtacgtttc
     3181 ttggacatgt ccggaaagca cttgatcgaa actccgacca aggcagacgg tgccgcgctc
     3241 tggagaatcg cccgcgattc gaagaagctc gatctcaaca cgccttacgc ctatttgctg
     3301 tggtgccacg atttcgccga cacctcggtc gtcgcgaaag tcgacggcgt cccggtcggc
     3361 ttcgtgatcg gataccgcaa gcaggacacc ggcttcgtct ggcaggtcgc ggtcgacgcg
     3421 tctcagcaag gaaaaggcct ggccggagcg ctgctcgacg agctgttcgg ccgtctgacc
     3481 gcccagggcg tgcggtacct cgaaaccacg atcaccccgg acaacgaggc ttcgatccgg
     3541 ttgttcgcct cgttcgccaa gcggtgggac accaccgtgg aacgccagga tctgttctcc
     3601 gcgacggatt tcccggccga ggaaggaacg catgagcagg aggacctgta ccgaatcggt
     3661 ccgctcagca gtcaatagaa cgtaaatccg gggatctcag ggagaaagaa aacgtgatga
     3721 gcattttcga ggaactcgaa tccgaagtac gcagttacag ccgtggctgg cccgtggtgt
     3781 tcgaccgggc ccaggggagc tacctgtacg acgaagacgg caaggcctat ctcgacttct
     3841 tcgcgggggc cggcgcgctc aactacgggc acaacaaccc ggcgctgaag cgggcgctga
     3901 tcgactacat cgcccgtgac ggcgtgacgc acgcgctcga catgttcacc gtggcgaaac
     3961 gggacttcct ccagacgttc caggagaaga tcctccagcc gcgcgatctc gactacaagg
     4021 tcgtgttccc cggccccggt ggcgcgaacg ccgtcgaggc cgcgctgaag ctcgcccgca
     4081 aggtgaccgg caaggaatcg gtcatcaact tcaccaacgc cttccacggg atgacgctgg
     4141 gcgccctctc ggtgaccggc aactcgatga agcgcggcgg cgcgggtgtc ccgctggtgc
     4201 acgccacccc gatgccgtac gacaactact tcgacggctc catcccggac ttcctctact
     4261 tcgagaagct gctcgaagac tccggcagcg gcctgaacga gccggccgcc gtgatcgtcg
     4321 aaggcgtgca gggcgaaggc ggcatcaacg ccgcccgtat cgagtggctc aaggcgctgg
     4381 acgacctgtg caagaagcac ggcatcctgc tgatcctcga cgacgtccag atgggctgcg
     4441 gccgcaccgg cccgttcttc agcttcgagg acgcgggcat caaacccgac atcgtctgcc
     4501 tctcgaagtc catcggcggt tacggcatcc cgatggcgct gacgctgatc cgcccggacc
     4561 tcgacgtctg ggagccgggt gagcacaacg gcaccttccg cggcatcagc ccggcgttcg
     4621 tcaccgcgac cgaggcgatc cgcgtctact ggagcgacga cgagctggag aagtcgacga
     4681 aggccaaggg cgagcgcatc gccgccgcct tccagggcat cgtcgaggcg tacccggacg
     4741 cgaacctgtt cgccaagggc cgcggcctcg cccggggtat cgagttcgcc aacggcgacc
     4801 tcgcgggcaa ggtctgcgcc gccgcgttcg agcgcgggct gctgatggag acctcgggcc
     4861 ccgacggcga ggtcatgaag gtcctgccgc cgctcaccct gacggacgac gagctcacga
     4921 agggcctgtc gatcatcgac gaggccgtcg ccactgtcct gaactgaccg ccccaaccag
     4981 gaacgagaag gagttttccg ttgcttgtca gaaccctcga cgaggtcacc gacaccgacg
     5041 ccgacatcaa gaccccgaac tggcgcagca agcgcatcgt gctggccaag gagggcgtcg
     5101 gcttctcggt gcacgagacc acgctgtacg ccgggacggt caacgacttc tggtacgcga
     5161 accacatcga agcggtgttc atcacctccg gtgaaggcga gctcgaaaac accgcgaccg
     5221 gcgaggtctt ccagctgaag ccgggcacgt tgtacctgct caacgaccac gacaagcacc
     5281 aggtccggcc caagaccgag atcaagtgcg tctgcgtgtt caacccgccc atcaccggcc
     5341 gtgaggtaca tgacgaaaac ggcgtgtacc cgctcgtcac cgaaccctag aaatcgctcg
     5401 aggaggcgac tgcattgacg ctgaccgaca cccgagtcga cgacagttac ccgacccgca
     5461 tcacgggtaa gcccgaacac ctgcccagga cgcaccccac cgtctggggc accgaggccg
     5521 acggtccgtt ggacgcggcc acgctcgcca accacgaaac gcgtggttac accgtcgtcg
     5581 accagttgct gtcgccgggc gaggtccaga cctactggca ggaactcgtc aggatgtcgt
     5641 cgggggacca cagcggcgac gaacgcgtga tcaccgaggc caagacaggt gaagtccgct
     5701 cgatcttcga cgtccacgag accagcgagc tgatcgcgga actcgtccgc gacccgcgcg
     5761 tgctcgaccg ggcccggcag atcctcggct ccgaggtcta catccaccag agccgcgtga
     5821 actacatgcc cggcttcaag ggcaccgggt tctactggca ctcggacttc gagacctggc
     5881 acgccgagga cggcatgccc gtgccccgcg cggtcagctg ctccatcgcg ctcacggaca
     5941 actacccgtt caacggcggg ctgatggtga tgccgggttc gcagcggacc ttcgtgcagt
     6001 gcgccgggga aaccccggaa gacaactaca agagttcgct gaaagagcag cgggtcggcg
     6061 tcccgagcga ggaggacatc accgcgctcg ccgccgaata cggcatcgac cagttcaccg
     6121 ggcaggccgg ttccgcgctc tggttcgact cgaacgtgat gcacggttcg tccaacaaca
     6181 tcacgccgta cccgaggtcg aacatcttcg tcgtgttcaa cagcgtcgag aacgcgttgc
     6241 aggagccgta cgccgcgatc gaaccgcgtc ccgccttcat cgccggccgc gactcgacgc
     6301 cgctgacgcg ctgacccgat ttgcgaaacc gcaggtcgcg aaggccatga cggggtgctt
     6361 gtagccgtcg gtgtgcgccc tgttacgttg tcgccacgtg agcgggccag tgggcccgct
     6421 cacgtaggtg ttgtcgacga ccgaacgggt tcttcgctcg gggtgagccc acccggacgg
     6481 acgtggacgc cgatgtccga tgtgggtcgc gcccccaggc ggcccaagtc ggacatcccc
     6541 cggctccgtg ccatcagatc gggactgatg gcacggagcc ttttttcgtt ccgtgaaggc
     6601 ctccttgagg gacccagagt ccctcaagga ggccttcacg ccttgccgag tacatgaagg
     6661 cccccttgct tgcgtctagc gcagtgaagg gatccttcat gtacttcacc tggtaatgag
     6721 cacctttgtc ctgaaggccc cctttgagac gttcaacgtc ccaaaggggg ccttcaggac
     6781 acgagcgccc cagcaagtcc acagtggaca gaagaaatct ggggcatttg tccgtatcga
     6841 gccttcacga cctgccgcgt tctggttggc gggcccttcg cgcggtaggt tggctactcc
     6901 ggtaagggga gagatcggag ggaggggggc ttgtcatggc cgaaagcggt tcccctgccc
     6961 gcttccagct cctcggtccc gtgcgcctcc tcgacggcga ccggccggtc cccgtcggcg
     7021 gacccggcgt ccgcggcctg ctcgcgctgc tggcgctgaa ggtcaacaag gtcgtcgcgc
     7081 tcgacgagat catcgacgcg ctctggggtc acgatccgcc cgccaccgcc cgcacgatcg
     7141 tccacggcaa cgtttcgcat ctgcgccgcg tgctccgcga catccaaggc gaccacccgc
     7201 gcggcgcccg gatcctcacc gcgccgcccg gctaccagct cacggtcgag cccgaccgga
     7261 tcgacgtcca ccgtgcccgg tccctgttgg agcgggcttc gacggaggag ccggacaagg
     7321 cgtccgaact gctcgcggag gccctctcgc tctggcaggg gcccgcgctg gccggcgtgc
     7381 cgggttcggt ccgcgccccc gaactggagg atctgcggct cgccgtccac ggtgccaggg
     7441 tcgacgcgga tctcgaactc ggcaggcacg cggaactgat cgtcgagctc agcccgctga
     7501 ttcgcgcgaa cccgctcgcc gagcgcaccg ccggtcagct gatgcgcgcg ctgtaccacg
     7561 ccgggcgccg cggagacgcg ctcgagctct accgcacggt gtcccgggcg acgctgcgca
     7621 ccctcggcgt cgaacccggc gcggacctgc gctggctgca cgaacgcgtc ctcaacgacg
     7681 acctgccggt caaggtcatc gcggaaccga agtccgagac ggcgcccgtc cagcagctgc
     7741 cgcccgcggt gccgaacctc gccgggcgcg aggcggacct ggcctggctc gacgagctcg
     7801 ccgagcgggc cgaagcgggc gagaccgcgg tcggcgtggt caccgggacc gccgggatcg
     7861 gcaagagcag cctggtggtg tggtgggcgc atcgggcggc ccgccgcttc cccgacggga
     7921 tcctgttcgc cgcgctgcgc ggtttcgacc cgcagcatcc accgttggag cccgcggagc
     7981 tgctcaccca gttcctgctc gggctcggcg tcccggccga gggcgtcccg gaacaggtcc
     8041 acgaacgcgt cgccctctac cggtcgatga tcgccggccg ccggatgctg gtgatcctcg
     8101 acaacgcccg ctccgccgag caggtccggc cgttgctgcc gcccggttcg cggtcgatgg
     8161 ccttggtgac cagccgttcc cgcctcgacg ggctcgccgt gtccaacgcc gccaaactcc
     8221 gggtgctcgg cacgctcgcg cccggtgacg ccgtccggtt gatcgaggaa ctcgccgggc
     8281 ccgccggcta cgacctcaac cacgcgctcg cccggctgtg cggttatctc ccgctggcgc
     8341 tgcggatcgc gggggcccgg ctcgccgcca gtgcgcagtg gtcggcgcag gatctggtcg
     8401 acgagctcgg caacgagcgc accaggctgg ccgcgctcga cgtcgaaggg ccggacgacg
     8461 gggtccgcgc ggcgttcgac gtctcgttcc gcggcctgcc gtccgaggtc gcggacacct
     8521 tcctgcggct cggcgtggtc cagtgcgttt cggccggttc gcatctgacg gccgcgatcg
     8581 gcgggatcac cgtcgccgag gcgcggcggc atctgcgggt gctggccgcg cacaacctgc
     8641 tcgccgaaac cggccgcgat tccttcgtcc cgcacgatct ggtccggctg ttcctccgcg
     8701 aacttgccga gaacgaactc gacgaggccg accgggaggc cgtgctgtcg cgttcggtgc
     8761 ggttctatca ggccgtcggc gaccgggccc ggcgcaagat gctccggatc gtcgaccccc
     8821 tcgatttcac cggcgtgctg accgacgcgc agacgccgcc gatcggttcc ttcgacgacg
     8881 cgcaggactg gttcacctcg gaatgggaga acctgctcga agtcctcgaa gccgcccgcg
     8941 ccgccggacg gcacgacgac gtctggcggc tggccagggt cgcgcacacc tatcgcgcgg
     9001 tgtacccgct gctggacgaa tggacgaggc tggtcgggat cgggctggag gccgccgagc
     9061 gatccggtga cgtcctcggc cggtgctgga tgctgatctc gcgatgcgcc atcgcgctca
     9121 ccttcgagct gccgcagggc tgcctcgccg acgccgaacg cgcgctggaa ctcgcgagcg
     9181 cgatcggcga cagcaggctg atggtctcgg cgaacatcca cctcggctcc gcgctgaccc
     9241 tcctcggctg gtacgacgag gcgatcggga cgttgcggca ggcggtcgag gagaccgagc
     9301 gcaccggcga cctcgaactc cgcgggcagg cactcaacaa ctgcgccgag gccgaaaagc
     9361 gggccggacg gttcatcgag gcgatcgggc atcaggtcgc ctcgctggag atcgaccgga
     9421 cccttggcga cgacagctac gtcgtcgtct cgctgaacaa tctcgccgaa ctgagcatgc
     9481 ggatcggcga actcgccgcg gccgaacggt acgtctggga ggccgtggac ctgacgatca
     9541 gcaggcggtt cgtcctccag gagggtgtcc tgcggctgac cttgggccgg gtgctgaggg
     9601 ccgggtcgga tgtggacggt gcccgcgagc agttcgcgct cgcgctgaag atcctcgcgg
     9661 acgccaatcc gaaactcgcg gcattggtcc gtgcggaact cacggacctc ggcgaaagtc
     9721 cgtgagtgat ccttagcgga ttcttagtgg cctcgccgca cacgcgcgta gggtccggtt
     9781 cagcgtcgat cgagccctag gggaggggca tgcggtaggg atcggcgctc gggcgggccc
     9841 ttctcggccc gtctgaagag ccgccaggag cggctcgtgg cggtccggcc ggtggaggtg
     9901 ggggcgatac cggccggccg ccaggcgggt ggtcagccgg tgccggcggt gcacagggcg
     9961 atggtctgcc ccttgttctc cgccaccttc tggccgttga ccaggatcgt gcaggcgatc
    10021 tggttgctga ccgacgagtt ccgggtgtcc gcggtgacgg tgagcaggta gtcgcccgag
    10081 ttgaaggacg ccgtgccgcg ccattcgtcg gtgctggccg gggtcgtctc ggtccgctgg
    10141 tcgttgagcg agccgaaccg gatggtcgcg ccgcccgagg cgctgacgac gaactcgacg
    10201 gtgttcttgt ccccggcgac ggccgggatg tgcaagcccg actcgccggt ggcgaagaaa
    10261 ctggtgtaga ccagtaaccc gcccgcggcc agcagcccgg cgatggaaag cgcgagcccc
    10321 gcgatcccca ttcccttctt gtccgcttcg cccttcttga ttttggtcaa accgatcacg
    10381 gaaagaatca
//
