LOCUS       NZ_FNJX01000001        10328 bp    DNA     linear   CON 10-DEC-2024
DEFINITION  Streptococcus equinus strain pGA-7, whole genome shotgun sequence.
ACCESSION   NZ_FNJX01000001
VERSION     NZ_FNJX01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptococcus equinus (Streptococcus bovis)
  ORGANISM  Streptococcus equinus
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900102715.1-RS_2024_12_09
            Annotation Date                   :: 12/09/2024 14:53:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.9
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,759
            CDSs (total)                      :: 1,711
            Genes (coding)                    :: 1,703
            CDSs (with protein)               :: 1,703
            Genes (RNA)                       :: 48
            rRNAs                             :: 2, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 38
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 18:06:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 196295
            Orig. end                         :: 206623
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNJX01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10328
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10328
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10328
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5328](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5328
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            491..1060
                     /db_xref="GeneID:63969617"
                     /gene="rnmV"
                     /locus_tag="BLR40_RS00910"
                     /old_locus_tag="SAMN04487837_0184"
     CDS             491..1060
                     /EC_number="3.1.26.8"
                     /GO_function="GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:63969617"
                     /gene="rnmV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081675.1"
                     /locus_tag="BLR40_RS00910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487837_0184"
                     /product="ribonuclease M5"
                     /protein_id="WP_074533801.1"
                     /transl_table=11
                     /translation="MTEKIKIQEVLVVEGKDDTANLRRFYDVDTYETRGSAINDDDLER
                     IEKLNDLRGVIVFTDPDYNGERIRKIIMNAIPNVKHAFLNRGEAVPKSKSKGRSLGVEH
                     ANFEDLQKALKGVLGNFDDDNQFDITRADLMRLGLLMGGDSRKRREYLGEELRIGYSNG
                     KQLLKRLELFGITLAEVEEAMEKYQA"
     gene            1171..2043
                     /db_xref="GeneID:63969618"
                     /gene="rsmA"
                     /locus_tag="BLR40_RS00915"
                     /old_locus_tag="SAMN04487837_0185"
     CDS             1171..2043
                     /EC_number="2.1.1.182"
                     /GO_function="GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:63969618"
                     /gene="rsmA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535644.1"
                     /locus_tag="BLR40_RS00915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487837_0185"
                     /product="16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="WP_021143191.1"
                     /transl_table=11
                     /translation="MRIADYSVTRAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEID
                     KNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLRDFDNVKVINEDILKS
                     DLQSRIKEFANPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMMQKEVADRISAE
                     PNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMTRREEPLVKVKDEDFF
                     FRVAKISFVHRRKTLWNNLTSHFGKAEETKAKLEKALEIAEIKPSIRGEALSIADFAKL
                     ADALKEVGL"
     misc_feature    2130..2140
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    2158..2172
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 22.3"
                     /tool="antismash"
     gene            2383..2565
                     /db_xref="GeneID:63971187"
                     /locus_tag="BLR40_RS08875"
                     /old_locus_tag="SAMN04487837_0186"
     CDS             2383..2565
                     /codon_start=1
                     /db_xref="GeneID:63971187"
                     /inference="COORDINATES: protein motif:HMM:NF015035.5"
                     /locus_tag="BLR40_RS08875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487837_0186"
                     /product="ComC/BlpC family leader-containing
                     pheromone/bacteriocin"
                     /protein_id="WP_074533472.1"
                     /transl_table=11
                     /translation="MNTKVFEQFDTMTTEALVCIEGGNQVFAGGGGKLKPDQPWWTKVD
                     WCSIASGVNGGMIVC"
     gene            2559..3251
                     /db_xref="GeneID:63969619"
                     /locus_tag="BLR40_RS00925"
                     /old_locus_tag="SAMN04487837_0187"
     CDS             2559..3251
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:63969619"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962520.1"
                     /locus_tag="BLR40_RS00925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487837_0187"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_234794538.1"
                     /sec_met_domain="Abi (E-value: 1.2e-13, bitscore: 47.1,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLNHLFLDDDRGKRVFFLTFPLIFYVIFFYLLPGLLRQVFIKYAT
                     DDFHKLIFLCSYFTFVFLLMVSYLLKQEGKKFFKVSFKYHYIGIIILLSLGNIVLSVVL
                     AYYLPLPMNQQLLNNSRLGASESSLLVKLLWGGVITLINEELFYRGILMTTYFNNSRYY
                     LDILVSTVCFASIHVIWSAWSWLDFVQYIPAGLSLGVTFRWTKSIYYPILLHALVNYLP
                     RIILVLLN"
     gene            3284..3958
                     /db_xref="GeneID:63969620"
                     /locus_tag="BLR40_RS00930"
                     /old_locus_tag="SAMN04487837_0188"
     CDS             3284..3958
                     /EC_number="3.4.-.-"
                     /codon_start=1
                     /db_xref="GeneID:63969620"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962518.1"
                     /locus_tag="BLR40_RS00930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487837_0188"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_074533473.1"
                     /sec_met_domain="Abi (E-value: 6.7e-17, bitscore: 57.5,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEKVKNLLAPKLLAIFFIYYHLFGLKFVMFTILERFDLPLTEYH
                     YPLLNIFTILFIIVLVIYLRRRRPEIFQIRPLKPLDYLVTLLAFGVIYAYFSNLLFVGG
                     DIKNDLLFFRDSPLAFLVALQPFIIGPILEELVYRGVLMTSFFEKSKFYLDCLLSAVMF
                     SVAHLSSYGLSLEVFKNYAFLGLILAILFRKTRSIYSPILVHMAWNIYLGWNIITFVFF
                     EK"
     gene            complement(<4455..4760)
                     /db_xref="GeneID:63969621"
                     /locus_tag="BLR40_RS00935"
                     /old_locus_tag="SAMN04487837_0189"
                     /pseudo=""
     CDS             complement(<4455..4760)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:63969621"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364968.1"
                     /locus_tag="BLR40_RS00935"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487837_0189"
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /pseudo=""
                     /sec_met_domain="Peptidase_C39 (E-value: 8.3e-33, bitscore:
                     108.9, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWRYKYISQIDMRDCGVAALASVAKHYGSHFSLAHLRELAKTSKE
                     GTTALGIVEAAKKIGFETRAIQANMELFEIPDIPYPCIVHVNKQGKLQHYYVVYKDK"
     misc_feature    complement(4867..4880)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: strong, score: 24.91"
                     /tool="antismash"
     misc_feature    complement(4897..4910)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 21.25"
                     /tool="antismash"
     gene            5001..5186
                     /db_xref="GeneID:63969622"
                     /locus_tag="BLR40_RS00940"
                     /old_locus_tag="SAMN04487837_0190"
     CDS             5001..5186
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:63969622"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021923.5"
                     /locus_tag="BLR40_RS00940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487837_0190"
                     /product="Blp family class II bacteriocin"
                     /protein_id="WP_074533474.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 1.9e-08,
                     bitscore: 30.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNLEQFAAMTEAELSTVEGGKKCNYSQVIFDGFMGAAGNVPGIV
                     IGGIGGAVLSYGGYCL"
     gene            5209..5328
                     /db_xref="GeneID:63971183"
                     /locus_tag="BLR40_RS08845"
                     /old_locus_tag="SAMN04487837_0191"
     CDS             5209..5328
                     /codon_start=1
                     /db_xref="GeneID:63971183"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLR40_RS08845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04487837_0191"
                     /product="bacteriocin"
                     /protein_id="WP_142349837.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 8.4e-09,
                     bitscore: 31.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQFKRMTEDDLSVVSGGSKLQCAVTQAGGALADLWKNT"
     misc_feature    5376..5390
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.08"
                     /tool="antismash"
     misc_feature    complement(5512..5532)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 24.15"
                     /tool="antismash"
     gene            6131..6397
                     /db_xref="GeneID:69986978"
                     /locus_tag="BLR40_RS08915"
     CDS             6131..6397
                     /codon_start=1
                     /db_xref="GeneID:69986978"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLR40_RS08915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_074533477.1"
                     /transl_table=11
                     /translation="MKKSVLKTTILLSAAVLSMATVSVFADDELVPVTEVTEVVDNTVE
                     NSAEQASDVQETQTSATNLETSDEAEESEVTIPQYEENISDFN"
     gene            6422..6742
                     /db_xref="GeneID:69986979"
                     /locus_tag="BLR40_RS08920"
     CDS             6422..6742
                     /codon_start=1
                     /db_xref="GeneID:69986979"
                     /inference="COORDINATES: protein motif:HMM:NF024498.5"
                     /locus_tag="BLR40_RS08920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="WP_074533478.1"
                     /transl_table=11
                     /translation="MFKEDGKEHVIYIGRPTCYYCRQFSSELKDFNILLNNRLEYYDTD
                     SQDFDEAAANFLFKTVGILGTPTIIRLQNGQIVSAWVGGGISGQELYDYLFNGKIPAAM
                     AE"
     gene            8550..9281
                     /db_xref="GeneID:63969624"
                     /locus_tag="BLR40_RS00975"
                     /old_locus_tag="SAMN04487837_0196"
     CDS             8550..9281
                     /codon_start=1
                     /db_xref="GeneID:63969624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295306.1"
                     /locus_tag="BLR40_RS00975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04487837_0196"
                     /product="response regulator transcription factor"
                     /protein_id="WP_074533480.1"
                     /transl_table=11
                     /translation="MLDIFILEDNITQQFRIERQIEAIMEKNNWAYQHLEAFAASEDII
                     ARASEKKSHQIFFLDLEIKGDEKQGIDVARAIREKDSTAIIVFVTTHSEFMLLTYQSLV
                     GAIDFIDKNLNDAAFEQRLELCLKEALKHQKGNLGENSYLFETAKARIRVAYDDILYFE
                     TSPTVHRVILHTKTGQTEFYGTIAEVAKSDERLFKCHRSFVINVNNVAEFDKSTRTAYF
                     ANGSYCQVSRDKVKSLMAEMR"
ORIGIN
        1 ggtgagattg gtcttgatta ttattggatg gaagacccag aagacgttca aattgaggtt
       61 ttcaaacgtc aaattcaact ttctaaagag cacaatctgc cttttatcgt gcacactcgt
      121 gatgctatgg aagatactta caaagttatc aaagaggtcg gtgttggacc acgtggtggg
      181 attattcact cattttctgg ttctttggaa atggcagagc gctttgtcga gcttggcatg
      241 atgatttcat tttcaggtgt tgtcacattt aaaaaagctc ttgatgttca agaagctgcg
      301 caaaaattac cacttgataa gattttggtt gagacagatg cgccttatct tgccccagtt
      361 ccaaaacgag gtcgtgaaaa tcgcacagct tacacacgct atgtagtgga taaaattgct
      421 gaacttcgtg gattaacaag cgaggaagta gcaaaagcaa catcagacaa tgcaaagagg
      481 ttatttaact gtgactgaaa aaattaaaat tcaagaagtc ctagtcgttg agggaaaaga
      541 cgatactgca aatttgcgcc gcttttacga cgtggacact tacgaaacac gaggctctgc
      601 tatcaatgat gatgaccttg aacgcattga aaaactaaat gaccttcgtg gcgttatcgt
      661 ctttacagac cctgattata atggcgaacg cattcgtaaa attatcatga atgctattcc
      721 aaatgtgaaa cacgcttttc taaatcgagg cgaagccgtt ccaaaatcaa aaagcaaagg
      781 tcgctcgctt ggagtagaac acgcaaattt cgaagatttg caaaaagcgc tcaaaggtgt
      841 tttaggaaac tttgatgatg acaatcaatt cgacatcaca agagctgatt tgatgcgctt
      901 aggacttttg atgggtggcg acagccgtaa acgtcgtgaa taccttggcg aagaactccg
      961 cattggctat agcaacggca aacaacttct taaacgctta gaactttttg gcattactct
     1021 agccgaagta gaagaagcga tggagaaata ccaagcataa ttcacatttc acgcttgact
     1081 cagatgtgat acaataagaa cttatattga tagaaatgaa aggaaagaaa tctttattct
     1141 tgggataacc tgtggataag gaaaaatact atgagaattg cagattacag cgtgacgcgt
     1201 gctgtccttg agcgtcatgg atttactttt aaaaaatcat ttggacagaa ctttttgaca
     1261 gacactaaca ttcttcaaaa gattgtggat actgctgaaa ttgacaaaaa cgtcaatgtt
     1321 attgaaatcg gtcctggtat cggtgctttg acagaatttt tggctgaaaa tgctgccgaa
     1381 gttatggcgt ttgaaatcga cgatcgtttg attccgattt tggctgatac gctccgcgat
     1441 tttgataatg tcaaagtgat taacgaagat atcttgaaat cagacctaca aagccgtatc
     1501 aaagaatttg ctaatccaga ccttccaatc aaggtcgtag caaatctgcc atactacatc
     1561 acaacaccaa tcttgatgca cttgattgaa tcaaaaattc catttgcgga atttgtcgtg
     1621 atgatgcaaa aagaagtggc agaccgcatt tcagcagagc caaacactaa agcttatggt
     1681 tcactatcaa ttgctgtgca atattacatg actgcaaaag ttgctttcat cgtgccacgt
     1741 acggtatttg tgccagcacc aaatgttgac tcagctattt tgaaaatgac tcgccgtgaa
     1801 gaaccacttg ttaaagtcaa agacgaagat ttcttcttcc gcgttgcgaa aatcagtttt
     1861 gttcaccgtc gtaaaacact ttggaacaat ttgacaagtc acttcggaaa agcagaggaa
     1921 acaaaagcaa aacttgaaaa agcacttgaa atcgcagaaa tcaagccatc tattcgaggc
     1981 gaagctcttt caattgcaga ctttgcaaaa ttagctgacg ctttaaaaga agtcggatta
     2041 taaatcaaaa accagctcat agagctggtt ttttagttta aatatgatta atgaaattca
     2101 ttaccgttga agcaatatcg tgccattgtt gcaatgtcat aaagtgataa atcaatgtga
     2161 aatacatttt ctcctaaact cccttgttaa taattaacat ttctattatg ccataaaatc
     2221 ttgtttataa acgcgaaagt cataaacggt caaaattcag tcgcgaatgg taaaaaatta
     2281 tgaaaaactt accgtttacg acattgacag ttattttgcg aaaattctga taaaataaat
     2341 cttgtcaatc gcgatgacaa aaataacttg ggagacattt acatgaatac aaaagtgttt
     2401 gaacaatttg acacaatgac aactgaagcc cttgtgtgta ttgaaggtgg aaatcaggtg
     2461 tttgctggag gaggcggtaa attaaaacct gatcaacctt ggtggacgaa agttgattgg
     2521 tgttcaattg caagtggcgt taatggagga atgattgttt gttgaatcat ctttttttag
     2581 atgatgatag aggaaagcga gtttttttcc tgacttttcc tcttattttt tatgttatct
     2641 ttttttacct tttgccagga ttattaagac aagtatttat aaaatatgct accgatgatt
     2701 ttcacaaact gatttttttg tgcagttatt ttacttttgt atttttgttg atggtgtcct
     2761 atttgcttaa gcaagagggg aaaaagtttt ttaaagtgag tttcaagtat cattatattg
     2821 ggataattat tttattgtct ttgggaaaca ttgttctttc tgttgttctt gcttattatt
     2881 tgccgttgcc aatgaaccag caattactga ataatagcag gttaggtgca tcagagagta
     2941 gtctacttgt gaagcttttg tggggaggcg tgattactct tataaatgaa gaactatttt
     3001 atcgcggtat tttgatgaca acttatttta ataattcacg ttactaccta gatattttag
     3061 tatctactgt ttgttttgcg tctatccatg ttatctggtc agcgtggtcg tggttagatt
     3121 ttgttcaata tattcctgcg ggactttctt taggtgtgac ttttaggtgg acaaaatcaa
     3181 tttattatcc aatattactg catgctttgg taaattatct tccaagaatt attttggttt
     3241 tactaaatta aatataaaat ttgatttcta aaaaggagta tttatgatag aaaaagtaaa
     3301 gaacttactt gctccaaaac tgttggcaat attttttatc tattatcatc tatttggatt
     3361 aaagtttgta atgtttacta ttttagagcg atttgattta ccattaacgg aatatcatta
     3421 ccctcttctt aatattttta ccatcctatt tattattgta ctggtgatat acctaagacg
     3481 aagaagacca gaaatatttc aaataagacc actaaaacct ttagattatt tagtaacttt
     3541 gcttgctttt ggggtgatat acgcttactt cagtaatctt ttatttgttg gtggagatat
     3601 aaaaaatgat ttattatttt tccgtgattc cccattggcg tttttggtcg cattacaacc
     3661 ctttattatc ggaccaatcc tagaagagtt agtttatcgt ggtgttttga tgactagctt
     3721 ttttgagaaa tcaaagtttt acttagattg cttactatct gctgtaatgt tctcagtagc
     3781 tcatctatct tcttatggtt tgagtttaga ggtatttaaa aactatgctt ttcttggttt
     3841 aattttagcg attcttttta gaaaaacacg gtcgatttac tcaccgattt tggttcacat
     3901 ggcgtggaat atttatcttg gatggaacat tattaccttt gtattctttg aaaagtagta
     3961 aaaatcatga aaaaacttac cgtttacgac gaaaaaacga ccgtttacga catttacgtt
     4021 tatttggcaa aatttctgat acaataaaat atgtcagtca catgacacgt catttccagg
     4081 agatttaaat gacaaaagga ctttaatcgc agtgttgtta agaaagtcgt tgcaataaaa
     4141 gggaaaactt agctacttgc gatacatggg agaaacctat ctttgaaaat tcatcatcta
     4201 taagatgata caaagagaaa gtaggctata aagcttactt tttttattga tgcgtacgct
     4261 taggttgtaa cagtgaaaat atttttggaa aaatacggtt ggattctatc tattattgca
     4321 gttacagcta tatgggcaag atatgttatt ctgttaattt tgaagtttgc taaatttaaa
     4381 ggaaaataac taagaatatt ttataaaaaa ccagctaatt gagctggctt ttcttattga
     4441 aaatctttta agagtttatc tttgtagacg acatagtaat gttgtaactt gccttgctta
     4501 ttgacatgga cgatacaggg ataaggaata tctggtattt caaaaagttc catatttgcc
     4561 tgaatggcgc gtgtttcaaa accaattttc tttgccgctt cgacgattcc aagagcagtt
     4621 gtgccttctt tactagtctt agccaattct ctcaagtgag caagcgaaaa gtgcgagcca
     4681 tagtgtttag cgaccgaagc aagagcagcc accccacaat ctctcatatc aatctgacta
     4741 atgtatttat atctccacat aaaaacattc cttctaaaac agagtcaaaa gaaattatgc
     4801 cacaaaatga gttatcgtga acacagatgt cgtaaacggt taaaattcag tcgcgaacgg
     4861 taaaaatttt tttgaaaaat tactgtttac gacgaaaaaa cgaccgtttg cgacatttac
     4921 gtttataagc aaaattttat gctaaactaa gaatcatcag ttgcaacgac aaataaaaaa
     4981 agggaaaacg gagaaaacaa atgaaaaatt tggaacaatt tgccgcaatg acagaggcag
     5041 agctttctac tgttgaaggc gggaaaaaat gtaattatag tcaagtaata tttgatggat
     5101 ttatgggagc agctgggaat gtacctggta tagttattgg tggaattggt ggagctgtac
     5161 tttcgtatgg tggttattgt ttataataaa atgagggaga gtattaaaat ggaacagttt
     5221 aaaagaatga cggaagatga cctatcagtt gttagtggtg gttctaaatt acaatgtgct
     5281 gttactcaag ctggaggggc tcttgctgat ttatggaaaa atacataaaa aaatacggtt
     5341 ggatcctatc tgctattgca gttacagcta tatggggaag atatgttata ttagaaaatt
     5401 tggttttccc ttataaaatt gtatacacca ttatatattt attatatgtt attttgttaa
     5461 ttttgaagtt tactaaattc aaaagaaaat aatttttata agttaaatct ttagttgtta
     5521 agatgactat aaacttttat ttaacggaat cggcagctag tttactaatt aaattcatga
     5581 tagctcaagt cattatatat gttctattct atttgtttga caaaaaacat agagagatgt
     5641 accttagatt atttataata acactactat ttacaataat tgtatatttt tcgtcataat
     5701 agtttagcta ttatataatc tattcttgaa aaacatagta ttattgttgc taatttagta
     5761 tatttcggtg ttactactgc agctggtgct tacttaggtt ataaaggtaa ttaattctta
     5821 tgtactattc tatttttgtt attttatatt gtattatttt agcagtaatt gctattgatg
     5881 gttttagtat taagaagagt agaaaaactt caacttatgt ttgctttttg attttttatt
     5941 ctttaaatca aaataaagat actactactc tattgtcgat agtttgttta cttattttga
     6001 tgttgattta ttatgaaaat agggatatca actgataaca attcaattga agtaatttat
     6061 taggtattac tattatgata gtcacagctg atagtttatt ctgtcagctt ttgttttgaa
     6121 gggagatttt atgaaaaaga gtgttttgaa aacaacaatt ttgttatcgg cagcggtttt
     6181 atcaatggca actgtttctg tctttgctga tgatgagctt gtgcctgtaa ctgaagtgac
     6241 agaagttgtg gataatactg tggaaaattc agctgagcag gcaagtgatg ttcaagagac
     6301 acaaacgagt gcgactaatt tagaaacatc agatgaggca gaagagtctg aagttactat
     6361 tccacagtat gaagaaaaca tctcggattt taattaagtt tcaatcacgg atgtatactc
     6421 tatgtttaaa gaagatggca aagagcacgt gatttatatt ggacgtccaa cttgttatta
     6481 ttgccgtcaa ttttcttcag agttaaagga ttttaatatt cttttgaata atcgtctgga
     6541 atattatgat acagacagtc aggatttcga tgaagctgcc gcgaatttct tgtttaaaac
     6601 agttggcatt ctaggaacac ctaccattat tcgtttgcaa aatggtcaaa tcgtgagtgc
     6661 ctgggttggt gggggaattt ctggtcaaga actttatgat tatctcttta atgggaaaat
     6721 cccagcggct atggcagaat aatcaagtga agaaaatgca gttagcacag aatctgtaat
     6781 tggagaaaca atggctagtt ctgaggctga taatgtgata aatgatttta ccgtttacga
     6841 cgaaaaaaca accgtttacg acatttacgt ttataagcaa aattttatgc taaactaaga
     6901 atcatcagtt gcaacggcaa aattatttgt gggaggtatt cagttgaagg tgggaatgtg
     6961 gttactaata ctgctggtgt tgctattgca ggaggtgtag taggtgtagc tttaggtgtg
     7021 gtattcccac attaaaataa aaaatatgaa tgaaaaacta ttaaaatttg gtaatatatt
     7081 agtcattata ttaactctaa ccacagatgt attatggttt atctctctaa gtatgatcag
     7141 tagagagaaa gtacagattt ttagtgctat gatattattt gtttttttga gttttgttat
     7201 tgatttattg gcacactatt ttctcaaaaa aaatagtatt gactataaat ggcacaggca
     7261 acaaataatc ataatttctt gtgcaactgg cttaagttta ttggttgtaa taattacttt
     7321 cttccaaaga gaaaatgtct taagcatgtt tctgatgctt gttgatagta ttttactact
     7381 cttttactac tcttttatcc taattcaata ggttataaaa ataacatcaa ttgaactaat
     7441 attgaaggaa ttgaaggttc aatccatcaa ttcctttatt ttataaagtc atagttttct
     7501 aaatggaatt ttatagtatt ctaaatttaa agtataaata aaaataatga ttttaaaagt
     7561 tttaccattt atgacctaaa agcaacaaat tacgacttgg acaaaagaag atgaaatatt
     7621 ctgataaaat ttgtcaatcg cgatgacaaa aaatattggg aggtattcaa atgaatacaa
     7681 aaacttttga acaatttgat gtaatgacag aggcagagcc ttcaactgtt gagggtggag
     7741 ttgctgtggg actcaattat gttaatcgtc actaaaaaat aatctaaagg aatagtgcag
     7801 aatatgaaag aaatttttga acatagatat gccattttat ttggtttatc aacgataatt
     7861 ggattttgtg ttggaattgt agttggatta aatagtataa attagataga gtatttggat
     7921 agttaataat aagtgtagag atttagcagc ccggcttaag tcgggttgta ttttaatttt
     7981 tgaaactaaa tagtttgtat atataatagt aatttttata aagtgtatag ttctttccta
     8041 ggattagctt tgataaagct taataaaggg aaagtcatat ctagcaaatt ataattttgc
     8101 taaaaattat cagagatgtt ttccaatgtt gtaatgacat ttcttgtttg aaatcttgat
     8161 aagactgctt atttttattg ttaggcattt gtatatctca tggcttgata gattgtcttg
     8221 agattttttg agaacaattt taagaaagga attttgaact aatgatgcaa aaattatttg
     8281 tagcgatttt gatagagttg ctattttacg ttgcgttgta ttttattgat agaagtcatc
     8341 gtcagatgta tcgtaggctt gctgtagtga cttttttagt tactttaatc gtctgttttt
     8401 cagcacttta aatcaatctg gtaggagtct atcagatttt attttttgga aaatgtcaat
     8461 tgtgacaggc ttgtcttttt ttacaggttt cctttataat agaaatgtta gtcttttaca
     8521 gattaagaaa attgtttagg gagagattta tgttagatat ttttatcctt gaggataata
     8581 tcacgcaaca atttcgtatt gagcgacaga tagaggcaat tatggaaaag aataattggg
     8641 cttatcagca cttggaagct tttgcggcat ctgaggatat tattgcaaga gcatctgaaa
     8701 agaagagcca tcagattttt tttcttgatt tagaaattaa aggggatgaa aagcaaggaa
     8761 ttgacgtggc aagagccatc cgtgagaagg attcaacagc tattattgtt tttgtaacga
     8821 ctcattctga gtttatgctt ttaacttatc agtcgttggt tggtgcgatt gatttcattg
     8881 ataaaaattt aaatgatgca gcttttgaac aacgtctaga gttatgcttg aaggaagctc
     8941 ttaagcatca aaaaggaaat ttaggtgaga attcttacct ttttgaaact gccaaagcga
     9001 gaattcgtgt agcttacgat gatattttat attttgaaac atcgccaacc gttcaccgag
     9061 taattttaca cacaaaaaca ggtcaaactg aattttacgg aacgattgcg gaggttgcca
     9121 aatcagatga gcgtctgttc aaatgtcacc gctcttttgt gattaatgta aataatgttg
     9181 ctgaatttga taaaagtacc cgaacagctt attttgcaaa tggctcttac tgccaagtct
     9241 cacgagacaa agtcaaaagc ctaatggcag aaatgaggtg aggggatgat tgattaccta
     9301 gtactctctt tgatagtaaa ctcgtttatt ctctttatat attctcaagt cagaggtgat
     9361 aaattaactc tttgggaaat attgatagca atcattgttt ttgtaggagt tgatcatact
     9421 tccttttact ttatagtatg caattttatt attttaatac tgctatctta ttttaaagat
     9481 agagaattac cagtatctct tcatgtattt tatggggttt tcccatggct agttgaaggg
     9541 gtttttagac gtttgatagg tttctttatc attccgctta taggtttaaa acatcaggat
     9601 gttggtggga ataatataat ttttatgtgt gttgagttag cgtctctaaa cctttatatg
     9661 ttactagtaa aaatttccaa atttgatttt caaaagttta tagagataat tagtgttaat
     9721 agattaagga aatttctcat tactactgat gtaacaatgg ttatttttta tgtggctatt
     9781 gaatttttga caggtatgga ggcttatgga ggagttaata atttaatata tcgccagtgg
     9841 ttagtcgtca tttatttcat tttctttatt ttgatgctat tttacatgaa ccgctcttac
     9901 caaaataagc ttgaaaaaga aattgcttca gcgcgtgagc aagagttgcg ttcgctgtct
     9961 gcttatagta aacagattga gggcttgtat gaagaattac gagcttttag acatgattat
    10021 gccaatattt tagccagttt gaaagagggc attgagcaga atgatatggg gattgttcga
    10081 catatttatg attctgtgtt gacggattca gctgatttta ttcaaaatag taagtttaac
    10141 attgggcgtt tagctaatat cgatgatgat gcgataaaaa gtttattgtc tgctaaattt
    10201 ttagaagctg aggctaatca tattgaggtc aatcttgaag ttaaagataa gattggtgca
    10261 cccgcaatgc cacttttgga ttacattcgt atactttcta ttttatgtga taatgcgatt
    10321 gaagctgc
//
