GCF_900101905.1 - 32 region(s)

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Location: 1 - 1,226 nt. (total: 1,226 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

BLU09_RS38015: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000342.4	0.64	NRPS	enniatin	Fusarium equiseti
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000342.4	0.64	NRPS	enniatin	Fusarium equiseti
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.64	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NZ_FNAJ01000017.1 - Region 1 - NRPS,RiPP-like

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Location: 1 - 70,547 nt. (total: 70,547 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: glycosyltransferase transferase

BLU09_RS31775: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

BLU09_RS31790: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Hydrolases: hydrolase

BLU09_RS31635: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Lyases: dehydratase

BLU09_RS31780: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

BLU09_RS31785: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase

BLU09_RS31680: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU09_RS31695: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU09_RS31700: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU09_RS31710: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU09_RS31725: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.48	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.46	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.45	NRPS	AM-toxin	Alternaria alternata
BGC0001249.5	0.44	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001128.4	0.43	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.43	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.42	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.42	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001166.5	0.41	NRPS	HC-toxin	Alternaria jesenskae
BGC0000342.4	0.40	NRPS	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.77	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.76	NRPS	AM-toxin	Alternaria alternata
BGC0001249.5	0.75	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.74	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001166.5	0.73	NRPS	HC-toxin	Alternaria jesenskae
BGC0000342.4	0.72	NRPS	enniatin	Fusarium equiseti

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 24.07 out of 30.11

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.83 out of 20.47

Confidence: medium

Additional weak hits (6):

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.20 out of 30.11

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.58 out of 32.74

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.65 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.20 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.02 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.21 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 70547:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - X - Val) + (D-Asp) + (D-Asn) + (X) + (X - Gly)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS31680: X - Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (24..423): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HWRAFDISVWEAHFFCGGEHHQYGPTEATIDASC
Nearest Stachelhaus code(s):: Gln DAWQFGLIDK (71% 8Å match); Hpg DIWHVGAITK (68% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1089..1484): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDIAGLELQAFCGGEWNLYGPTETTIWSAV
Nearest Stachelhaus code(s):: Gly DILQFGLIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS31695: X

AMP-binding (469..882): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: HGMAFDISVWQAEVIPTGELNAYGPTETSDDTNL
Nearest Stachelhaus code(s):: Dpr DIWEMTADDK (74% 8Å match); Dab DIWELTADDK (68% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLU09_RS31700: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (536..938): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWCSFDLTVTSAKLVIGGEINEYGPTETVVGCCV
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS31710: Asp

Search NORINE for peptide: strict or relaxed

AMP-binding (463..865): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YSVSADLGNTVPKIILGGEYNHYGPTETTVGVMT
Nearest Stachelhaus code(s):: Asp DLTKIGHVGK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS31725: fatty_acid - X - Val

Search NORINE for peptide: strict or relaxed

CAL_domain (44..488): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 32.7
NH2: 30.5
AHBA: 15.1
Acetyl-CoA: 5.3
shikimic_acid: 0.0

AMP-binding (1161..1563): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAENFDSSVEELDLIVAGEINVYGPTEATVSATC
Nearest Stachelhaus code(s):: Lys DAEDIGTVSK (71% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2219..2618): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LSPAFDAATLEGWLLAGGDINGYGPTENTTFTCC
Nearest Stachelhaus code(s):: Val DALWLGGTFK (91% 8Å match); bOH-Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
CelR	Cellobiose uptake repressor
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNAJ01000017.1 - Region 2 - terpene-precursor

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Location: 90,998 - 111,891 nt. (total: 20,894 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthase synthetase

BLU09_RS31930: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

BLU09_RS31935: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000665.5	0.37	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000668.5	0.36	other (other), terpene	12-epi-hapalindole C isonitrile, 12-epi-hapalindole E, 12-epi-fischerindole U isonitrile, fischerindole L, 12-epi-fischerindole I isonitrile, welwitindolinone A isonitrile, welwitindolinone B isothiocyanate, welwitindolinone C isothiocyanate, N-methylwelwitindolinone C isothiocyanate, N-methylwelwitinsolinone C isonitrile, 3-epi-welwitindolinone B isothiocyanate, 3-(Z-2'-isocyanoethenyl)-indole	Hapalosiphon welwitschii UTEX B 1830
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000643.3	0.36	terpene	carotenoid	Brevundimonas vesicularis
BGC0001605.3	0.36	terpene	gibberellin	Sinorhizobium fredii NGR234

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002283.2	0.49	terpene	sodorifen	Serratia plymuthica
BGC0001361.3	0.49	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002397.2	0.47	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.44	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.39	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000665.5	0.37	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000668.5	0.36	other (other), terpene	12-epi-hapalindole C isonitrile, 12-epi-hapalindole E, 12-epi-fischerindole U isonitrile, fischerindole L, 12-epi-fischerindole I isonitrile, welwitindolinone A isonitrile, welwitindolinone B isothiocyanate, welwitindolinone C isothiocyanate, N-methylwelwitindolinone C isothiocyanate, N-methylwelwitinsolinone C isonitrile, 3-epi-welwitindolinone B isothiocyanate, 3-(Z-2'-isocyanoethenyl)-indole	Hapalosiphon welwitschii UTEX B 1830
BGC0002309.2	0.36	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000643.3	0.36	terpene	carotenoid	Brevundimonas vesicularis
BGC0001605.3	0.36	terpene	gibberellin	Sinorhizobium fredii NGR234

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor		FPP	C15	acyclic	PP

Genes with terpene domains:

BLU09_RS31935: PT_FPPS_like

Domain 1 (37 - 296): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: Farnesyl diphosphate synthase, bacterial

Reactions:: DMAPP, IPP → FPP

Transcription Factor Binding Sites

Binding sites found:

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 21.84 out of 21.84

Confidence: strong

Additional weak hits (3):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.71 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.29 out of 21.55

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.10 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
MexT	Global virulence regulator

NZ_FNAJ01000022.1 - Region 1 - NRPS-like

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Location: 85,056 - 107,596 nt. (total: 22,541 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: esterase

BLU09_RS39965: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: dehydratase

BLU09_RS34785: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.62	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.62	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.62	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (6):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.27 out of 29.25

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.55 out of 28.14

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.96 out of 26.33

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.72 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.98 out of 29.65

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.40 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 85055 - 107596:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS39965: X

AMP-binding (3..382): X

nrpys: bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGSAFDASTLEGWLLAGGDLNGYGPTEGTTFTTV
Nearest Stachelhaus code(s):: bOH-Val DALWLGGTFK (88% 8Å match); Val DALWLGGTFK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description

NZ_FNAJ01000016.1 - Region 1 - NRPS,T1PKS

Download region SVG

Location: 38,272 - 130,925 nt. (total: 92,654 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acylase

BLU09_RS31355: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLU09_RS31360: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLU09_RS31370: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Ligases: ligase synthase

BLU09_RS31350: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU09_RS31365: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BLU09_RS31375: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BLU09_RS31385: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	1.19	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	1.18	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	1.17	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	1.17	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001135.5	1.00	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	1.00	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001164.3	0.98	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.97	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001758.4	0.95	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000307.4	0.95	NRPS	AbT1	Aureobasidium pullulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.75	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.75	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.75	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001187.4	0.69	NRPS, PKS	xenolozoyenone	Glarea lozoyensis
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001737.3	0.68	NRPS, PKS	phenalamide	Corallococcus coralloides
BGC0001022.5	0.68	NRPS, PKS	myxalamid, myxalamid A, myxalamid C, myxalamid D	Stigmatella aurantiaca
BGC0001164.3	0.67	PKS	1-heptadecene	Cyanothece sp. PCC 7822

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (13):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.56 out of 27.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.90 out of 24.78

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.85 out of 18.57

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.50 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.92 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.18 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.48 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.06 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.11 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.25 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.93 out of 33.14

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.67 out of 20.01

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.81 out of 25.88

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 38271 - 130925:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - X) + (Me-ccmal) + (Me-ccmal) + (pk - pk - ccmal) + (Me-redmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 38271 - 122991:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - X) + (Me-ccmal) + (Me-ccmal) + (pk - pk - ccmal) + (Me-redmal) + (Ala)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 84460 - 130925:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - X) + (Me-ccmal) + (Me-ccmal) + (pk - pk - ccmal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS31350: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (542..932): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: FWATFDLSVFETNTNLAGEFNLYGPSETTTYSTF
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS31355: mmal

PKS_AT (545..843): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 79.2%; Methoxymalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 180.6
Ethylmalonyl-CoA: 155.9
Methoxymalonyl-CoA: 139.1
Malonyl-CoA: 107.5
Isobutyryl-CoA: 99.3
2-Methylbutyryl-CoA: 87.6
Acetyl-CoA: 87.0
Benzoyl-CoA: 86.6
trans-1,2-CPDA: 83.0
CHC-CoA: 82.3
Propionyl-CoA: 79.5
3-Methylbutyryl-CoA: 52.9
fatty_acid: 43.3
inactive: 22.3

BLU09_RS31360: pk - pk - mal

PKS_AT (561..859): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; Methylmalonyl-CoA: 83.3%; Ethylmalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 141.5
Ethylmalonyl-CoA: 131.6
Malonyl-CoA: 128.9
Methoxymalonyl-CoA: 115.8
2-Methylbutyryl-CoA: 84.9
Isobutyryl-CoA: 84.6
Acetyl-CoA: 76.3
trans-1,2-CPDA: 67.6
Propionyl-CoA: 56.8
Benzoyl-CoA: 56.8
CHC-CoA: 45.7
inactive: 40.2
3-Methylbutyryl-CoA: 37.8
fatty_acid: 27.2

PKS_AT (2371..2667): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; Methylmalonyl-CoA: 75.0%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 131.0
Malonyl-CoA: 125.3
Methoxymalonyl-CoA: 122.9
Isobutyryl-CoA: 110.3
Ethylmalonyl-CoA: 96.6
Benzoyl-CoA: 69.6
Propionyl-CoA: 67.0
Acetyl-CoA: 62.5
2-Methylbutyryl-CoA: 60.9
CHC-CoA: 57.1
inactive: 53.5
fatty_acid: 52.5
3-Methylbutyryl-CoA: 50.4
trans-1,2-CPDA: 41.7

PKS_AT (3894..4187): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 213.7
inactive: 174.8
Methoxymalonyl-CoA: 88.5
Methylmalonyl-CoA: 84.3
Propionyl-CoA: 66.3
2-Methylbutyryl-CoA: 52.9
Isobutyryl-CoA: 51.6
Ethylmalonyl-CoA: 49.4
CHC-CoA: 41.0
trans-1,2-CPDA: 37.2
Benzoyl-CoA: 34.6
Acetyl-CoA: 25.3
3-Methylbutyryl-CoA: 24.4
fatty_acid: 20.7

BLU09_RS31365: mmal

PKS_AT (568..865): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 100.0%; Malonyl-CoA: 87.5%; Ethylmalonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 173.5
Ethylmalonyl-CoA: 154.9
Methoxymalonyl-CoA: 127.3
Benzoyl-CoA: 101.8
Malonyl-CoA: 93.9
trans-1,2-CPDA: 92.3
Isobutyryl-CoA: 85.2
2-Methylbutyryl-CoA: 76.5
Acetyl-CoA: 76.1
Propionyl-CoA: 65.8
CHC-CoA: 54.1
inactive: 42.6
3-Methylbutyryl-CoA: 37.2
fatty_acid: 21.7

BLU09_RS31370: mmal

PKS_AT (563..859): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Ethylmalonyl-CoA: 83.3%; Malonyl-CoA: 79.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 176.4
Ethylmalonyl-CoA: 154.4
Methoxymalonyl-CoA: 136.3
Isobutyryl-CoA: 102.0
Benzoyl-CoA: 93.6
2-Methylbutyryl-CoA: 90.1
Malonyl-CoA: 89.7
trans-1,2-CPDA: 71.2
Propionyl-CoA: 61.3
CHC-CoA: 56.2
Acetyl-CoA: 54.1
3-Methylbutyryl-CoA: 43.9
inactive: 36.8
fatty_acid: 22.3

BLU09_RS31375: pk - mmal

PKS_AT (606..903): pk

ATSignature: Isobutyryl-CoA

Top 3 matches:: Isobutyryl-CoA: 66.7%; Malonyl-CoA: 54.2%; Methylmalonyl-CoA: 54.2%

minowa: Isobutyryl-CoA

Prediction, score:

Isobutyryl-CoA: 150.6
Benzoyl-CoA: 127.1
Methylmalonyl-CoA: 102.5
Methoxymalonyl-CoA: 84.2
Ethylmalonyl-CoA: 82.6
Malonyl-CoA: 79.1
2-Methylbutyryl-CoA: 58.7
Acetyl-CoA: 50.0
Propionyl-CoA: 49.0
CHC-CoA: 37.3
trans-1,2-CPDA: 34.5
3-Methylbutyryl-CoA: 25.3
inactive: 23.1
fatty_acid: 16.3

PKS_AT (1032..1327): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 91.7%; Ethylmalonyl-CoA: 79.2%; Malonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 186.0
Ethylmalonyl-CoA: 166.8
Methoxymalonyl-CoA: 150.5
Isobutyryl-CoA: 113.2
Benzoyl-CoA: 112.3
2-Methylbutyryl-CoA: 103.2
Malonyl-CoA: 102.5
trans-1,2-CPDA: 87.6
Propionyl-CoA: 84.7
CHC-CoA: 66.1
Acetyl-CoA: 56.1
fatty_acid: 39.7
3-Methylbutyryl-CoA: 39.4
inactive: 26.7

BLU09_RS31385: fatty_acid - X

CAL_domain (47..499): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 25.6
AHBA: 16.7
NH2: 15.9
Acetyl-CoA: 0.3
shikimic_acid: 0.0

A-OX (1199..1969): X

No predictions made

TFBS definitions

Regulator	Description

NZ_FNAJ01000011.1 - Region 1 - NRP-metallophore,NRPS

Download region SVG

Location: 1 - 49,127 nt. (total: 49,127 nt) Show pHMM detection rules used

Region on contig edge.

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidase

BLU09_RS26750: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BLU09_RS26785: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

Transferases: aminotransferase

BLU09_RS26715: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: hydrolase

BLU09_RS26735: isochorismatase

smcogs: SMCOG1027: isochorismatase

Lyases: dehydratase

BLU09_RS26730: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Isomerases: isomerase racemase

BLU09_RS26745: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

BLU09_RS26775: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

Ligases: ligase

BLU09_RS26695: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU09_RS26740: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002492.3	1.62	NRPS	myxochelin B, myxochelin A	Myxococcus xanthus DK 1622
BGC0001185.5	1.01	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0001132.4	0.89	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000343.5	0.88	NRPS	enterobactin	Pseudomonas sp. J465
BGC0000401.5	0.86	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002075.3	0.84	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002286.3	0.84	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.84	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001249.5	0.82	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001825.4	0.82	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002492.3	0.93	NRPS	myxochelin B, myxochelin A	Myxococcus xanthus DK 1622
BGC0001185.5	0.79	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0001132.4	0.75	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000343.5	0.75	NRPS	enterobactin	Pseudomonas sp. J465
BGC0000401.5	0.74	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002075.3	0.73	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002286.3	0.73	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001825.4	0.73	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 27.11 out of 29.38

Confidence: strong

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.31 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 25.65 out of 29.38

Confidence: medium

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.33 out of 29.38

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 24.73 out of 28.19

Confidence: strong

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.38 out of 28.19

Confidence: medium

Additional weak hits (27):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.06 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.97 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.90 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 21.64 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.95 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.60 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.03 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.24 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.39 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.04 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 20.43 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.66 out of 32.74

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.92 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 17.61 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.95 out of 28.14

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 21.08 out of 26.12

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.74 out of 30.22

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.34 out of 36.54

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.79 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.77 out of 27.78

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.79 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.06 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.45 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 28.19

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.14 out of 30.11

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.04 out of 30.11

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.26 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 49127:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Lys) + (Val - X - X - Ala)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS26695: Val - X - X - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (1..216): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: ---AFDASTLEGWLLAGGDINGYGPTENTTFSST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (79% 8Å match); bOH-Val DALWLGGTFK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (877..1281): X

nrpys: OH-Asp, Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YGVSADLGHTAPKIVLGGDFNHYGPSETTVGVLT
Nearest Stachelhaus code(s):: OH-Asp DLTKVGHVGK (82% 8Å match); Asp DLTKVGHVGK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1943..2339): X

nrpys: Val, bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGTAFDASTLEGWLLAGGDINGYGPTENTTFSST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (82% 8Å match); bOH-Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3002..3395): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: FWTTFDLSVFEANTNLAGELNLYGPTEDTTYSTW
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS26730: Lys

Search NORINE for peptide: strict or relaxed

AMP-binding (456..860): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGAAFDASVEEVDLIIGGELNTYGPTEATVVATV
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS26740: X

AMP-binding (35..440): X

nrpys: diOH-Bz, Sal

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SGSLFPLSSPGGAVQVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz or Sal PLPAQGVVNK (97% 8Å match); diOH-Bz PLPAQGVVNK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
FuR variant 1	Ferric Uptake Regulator
ZuR variant 1	Zinc-responsive repressor

NZ_FNAJ01000011.1 - Region 2 - terpene-precursor

Download region SVG

Location: 57,982 - 78,980 nt. (total: 20,999 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: glucanase glucosidase

BLU09_RS26915: Pullulanase, type I

smcogs: SMCOG1297: Pullulanase, type I

BLU09_RS26925: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

BLU09_RS26930: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Ligases: synthetase

BLU09_RS26895: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.45	terpene	astallatene	Arabidopsis thaliana
BGC0002285.2	0.44	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002400.2	0.42	terpene	boleracene	Brassica oleracea var. oleracea
BGC0001285.5	0.40	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0000676.5	0.39	terpene	aphidicolin, aphidicolan-16β-ol, 3-deoxyaphidicolin, 17-deoxyaphidicolin	Phoma betae
BGC0001075.5	0.38	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0000678.5	0.37	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000905.5	0.37	other (other)	heme D1	Heliobacillus mobilis
BGC0002398.2	0.37	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000906.3	0.37	other (other)	heme D1	Heliophilum fasciatum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.45	terpene	astallatene	Arabidopsis thaliana
BGC0002285.2	0.44	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0002400.2	0.42	terpene	boleracene	Brassica oleracea var. oleracea
BGC0001285.5	0.40	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0000676.5	0.39	terpene	aphidicolin, aphidicolan-16β-ol, 3-deoxyaphidicolin, 17-deoxyaphidicolin	Phoma betae
BGC0001075.5	0.38	PKS, terpene	aurachin RE	Rhodococcus erythropolis
BGC0000678.5	0.37	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0000905.5	0.37	other (other)	heme D1	Heliobacillus mobilis
BGC0002398.2	0.37	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0000906.3	0.37	other (other)	heme D1	Heliophilum fasciatum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

BLU09_RS26895: PT_FPPS_like

Domain 1 (42 - 305): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 24.59 out of 32.93

Confidence: medium

Additional weak hits (3):

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.25 out of 29.51

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.59 out of 32.93

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.45 out of 18.57

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
LexA variant 3	Repressor of DNA damage response

NZ_FNAJ01000011.1 - Region 3 - RRE-containing

Download region SVG

Location: 111,053 - 131,349 nt. (total: 20,297 nt) Show pHMM detection rules used

RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: kinase

BLU09_RS27110: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001645.3	0.42	ribosomal	lagmysin	Streptomyces sp. NRRL S-118
BGC0002674.2	0.40	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0001364.3	0.39	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0001887.3	0.39	ribosomal	huazacin	Bacillus thuringiensis serovar huazhongensis BGSC 4BD1
BGC0001655.3	0.38	ribosomal	LP2006	Nocardiopsis alba DSM 43377
BGC0000496.5	0.37	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001660.3	0.37	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0000626.3	0.36	ribosomal	thuricin	Bacillus thuringiensis
BGC0000563.4	0.36	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001645.3	0.42	ribosomal	lagmysin	Streptomyces sp. NRRL S-118
BGC0002674.2	0.40	ribosomal	enterocin F4-9	Enterococcus faecalis
BGC0000616.4	0.40	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0001364.3	0.39	ribosomal	cinnamycin B	Actinomadura atramentaria DSM 43919
BGC0001887.3	0.39	ribosomal	huazacin	Bacillus thuringiensis serovar huazhongensis BGSC 4BD1
BGC0001655.3	0.38	ribosomal	LP2006	Nocardiopsis alba DSM 43377
BGC0000496.5	0.37	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001660.3	0.37	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0000626.3	0.36	ribosomal	thuricin	Bacillus thuringiensis
BGC0000563.4	0.36	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.34 out of 27.55

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.13 out of 23.79

Confidence: medium

Additional weak hits (3):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.27 out of 20.01

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.17 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.15 out of 24.78

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 12 - 92; Score: 42.4; RRE type: PqqD_RRE; Location: 5 - 92; Score: 30.5

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
GnfM	Regulator of nitrogen-fixation genes
IolR	Regulator of the inositol catabolism

NZ_FNAJ01000011.1 - Region 4 - NRPS,T1PKS

Download region SVG

Location: 160,161 - 234,542 nt. (total: 74,382 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU09_RS27480: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acetyltransferase

BLU09_RS27465: acetyl-CoA acetyltransferase

smcogs: SMCOG1124: acetyl-CoA acetyltransferase

Ligases: ligase synthase

BLU09_RS27380: carbamoyl phosphate synthase small subunit

smcogs: SMCOG1249: carbamoyl phosphate synthase small subunit

BLU09_RS27400: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS27405: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000393.5	0.99	NRPS, PKS	myxoprincomide-c506	Myxococcus xanthus DK 1622
BGC0001758.4	0.98	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.97	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.94	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.90	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.89	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.88	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.88	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.86	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002071.2	0.85	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000393.5	0.83	NRPS, PKS	myxoprincomide-c506	Myxococcus xanthus DK 1622
BGC0001758.4	0.78	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.78	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.77	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.75	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001128.4	0.75	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.75	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.75	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.74	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002071.2	0.74	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (8):

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.72 out of 20.97

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.62 out of 26.12

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.53 out of 27.55

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.61 out of 25.88

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.79 out of 27.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.35 out of 24.78

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 22.58 out of 30.11

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.78 out of 26.12

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 160160 - 234542:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - Ser - Val - mal) + (Ser - Tyr - bLys - Ala - Ser)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS27400: fatty_acid - Ser - Val - mal - X

Search NORINE for peptide: strict or relaxed

CAL_domain (41..490): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 24.3
AHBA: 13.8
NH2: 11.4
shikimic_acid: 5.1
Acetyl-CoA: -2.6

AMP-binding (1162..1563): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAVDVTY
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2229..2628): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSEAFDGSVMELHFHVVGDTNGYGPTENSIISAS
Nearest Stachelhaus code(s):: Val DGMHNVGIIK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

PKS_AT (3364..3660): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 140.4
Methoxymalonyl-CoA: 76.3
Methylmalonyl-CoA: 61.9
inactive: 57.5
Propionyl-CoA: 38.7
Isobutyryl-CoA: 34.2
Ethylmalonyl-CoA: 31.3
CHC-CoA: 28.0
Benzoyl-CoA: 27.1
Acetyl-CoA: 24.9
2-Methylbutyryl-CoA: 23.6
trans-1,2-CPDA: 17.0
fatty_acid: 14.2
3-Methylbutyryl-CoA: 11.1

A-OX (4386..5110): X

No predictions made

BLU09_RS27405: Ser - Tyr - bLys - Ala - Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (723..1124): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFVCSGEHNLYGPTEAAVDVTY
Nearest Stachelhaus code(s):: Ser DVWHVSLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1783..2183): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MGTAFDGALEPHDCVCAGEFNAYGPTESTVCATT
Nearest Stachelhaus code(s):: Tyr DGEDVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2847..3254): bLys

nrpys: bLys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAAAWDTSAEEPNLVIGGELNTYGLTEVTAVATS
Nearest Stachelhaus code(s):: bLys DTENVGTAVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3918..4318): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGMAFDGTVERHDCFCGGEFNVYGPTESTVCATS
Nearest Stachelhaus code(s):: Ala DGEDFGVVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4977..5377): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LASAFDVSMDQHDCLCAGEFNAYGPTESTVYATY
Nearest Stachelhaus code(s):: Ser DVGYLAAVYK (50% 8Å match)
Stachelhaus code match:: 80% (moderate)

TFBS definitions

Regulator	Description

NZ_FNAJ01000007.1 - Region 1 - T1PKS

Download region SVG

Location: 151,663 - 198,313 nt. (total: 46,651 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

BLU09_RS21460: oxidoreductase

smcogs: SMCOG1079: oxidoreductase

BLU09_RS21465: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: acylase methyltransferase

BLU09_RS21470: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLU09_RS21480: methyltransferase

smcogs: SMCOG1089: methyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000393.5	0.42	NRPS, PKS	myxoprincomide-c506	Myxococcus xanthus DK 1622
BGC0000916.5	0.42	other (other)	molybdenum cofactor	Staphylococcus carnosus
BGC0001909.3	0.41	PKS	strobilurin A	Strobilurus tenacellus
BGC0000906.3	0.41	other (other)	heme D1	Heliophilum fasciatum
BGC0000905.5	0.41	other (other)	heme D1	Heliobacillus mobilis
BGC0000883.5	0.39	other (other)	arginomycin	Streptomyces arginensis
BGC0000188.5	0.39	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0000662.5	0.39	terpene	grixazone A	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000885.3	0.39	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000889.5	0.39	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000393.5	0.42	NRPS, PKS	myxoprincomide-c506	Myxococcus xanthus DK 1622
BGC0000916.5	0.42	other (other)	molybdenum cofactor	Staphylococcus carnosus
BGC0001909.3	0.41	PKS	strobilurin A	Strobilurus tenacellus
BGC0000906.3	0.41	other (other)	heme D1	Heliophilum fasciatum
BGC0000905.5	0.41	other (other)	heme D1	Heliobacillus mobilis
BGC0000883.5	0.39	other (other)	arginomycin	Streptomyces arginensis
BGC0000188.5	0.39	other (other)	saxitoxin, neosaxitoxin, decarbamoylsaxitoxin, gonyautoxin 3, gonyautoxin 2	Cylindrospermopsis raciborskii T3
BGC0000662.5	0.39	terpene	grixazone A	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000885.3	0.39	other (other)	4-hydroxy-3-nitrosobenzamide	Streptomyces murayamaensis
BGC0000889.5	0.39	other (other)	bagremycin A, bagremycin B	Streptomyces sp. WK-5344

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.31 out of 18.46

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.58 out of 23.79

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.80 out of 23.79

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.67 out of 20.97

Confidence: medium

Additional weak hits (9):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.91 out of 26.85

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.21 out of 30.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.08 out of 28.19

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.71 out of 32.93

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.68 out of 32.93

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.44 out of 18.57

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.22 out of 26.12

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.29 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.57 out of 28.19

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

BLU09_RS21470: mal

PKS_AT (213..509): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 48.3
Methylmalonyl-CoA: 40.9
Methoxymalonyl-CoA: 29.1
trans-1,2-CPDA: 27.8
Ethylmalonyl-CoA: 25.5
Propionyl-CoA: 20.3
2-Methylbutyryl-CoA: 20.0
CHC-CoA: 19.5
Acetyl-CoA: 16.0
Isobutyryl-CoA: 13.9
fatty_acid: 9.8
Benzoyl-CoA: 9.6
inactive: 0.0
3-Methylbutyryl-CoA: 0.0

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CcpA	Catabolite control protein A
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FNAJ01000007.1 - Region 2 - RiPP-like

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Location: 273,687 - 284,550 nt. (total: 10,864 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001838.4	0.29	NRPS	WLIP	Pseudomonas putida
BGC0001588.3	0.24	saccharide	exopolysaccharides	Burkholderia pseudomallei 1026b
BGC0000714.5	0.22	saccharide	sisomicin	Micromonospora inyonensis
BGC0001603.3	0.22	saccharide	gentamicin	Micromonospora echinospora
BGC0002070.3	0.22	PKS	SEK90, pentangumycin	Saccharothrix espanaensis DSM 44229
BGC0001589.3	0.22	saccharide	exopolysaccharides	Burkholderia cenocepacia J2315
BGC0002025.4	0.21	NRPS, PKS	thaxteramide C	Jahnella sp. MSr9139
BGC0001093.3	0.21	NRPS, PKS	disorazol A	Sorangium cellulosum
BGC0000080.5	0.21	PKS	jerangolid A, jerangolid D	Sorangium cellulosum
BGC0000696.5	0.21	saccharide	gentamicin	Micromonospora echinospora

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001838.4	0.29	NRPS	WLIP	Pseudomonas putida
BGC0001588.3	0.24	saccharide	exopolysaccharides	Burkholderia pseudomallei 1026b
BGC0000714.5	0.22	saccharide	sisomicin	Micromonospora inyonensis
BGC0001603.3	0.22	saccharide	gentamicin	Micromonospora echinospora
BGC0002070.3	0.22	PKS	SEK90, pentangumycin	Saccharothrix espanaensis DSM 44229
BGC0001589.3	0.22	saccharide	exopolysaccharides	Burkholderia cenocepacia J2315
BGC0002025.4	0.21	NRPS, PKS	thaxteramide C	Jahnella sp. MSr9139
BGC0001093.3	0.21	NRPS, PKS	disorazol A	Sorangium cellulosum
BGC0000080.5	0.21	PKS	jerangolid A, jerangolid D	Sorangium cellulosum
BGC0000696.5	0.21	saccharide	gentamicin	Micromonospora echinospora

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.35 out of 23.79

Confidence: medium

Additional weak hits (1):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
IolR	Regulator of the inositol catabolism

NZ_FNAJ01000007.1 - Region 3 - NRPS,NRPS-like,T1PKS

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Location: 344,585 - 449,797 nt. (total: 105,213 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acylase acyltransferase glycosyltransferase sulfotransferase

BLU09_RS22265: glutathione S-transferase

smcogs: SMCOG1193: glutathione S-transferase

BLU09_RS22295: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

BLU09_RS22305: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLU09_RS22320: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

Hydrolases: esterase

BLU09_RS22290: thioesterase

smcogs: SMCOG1004: thioesterase

BLU09_RS39465: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

BLU09_RS22240: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU09_RS22245: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS22250: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS22255: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BLU09_RS22300: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BLU09_RS22325: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	2.22	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	2.14	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	2.10	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	2.07	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	2.04	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	2.04	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	2.00	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	2.00	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	1.89	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	1.87	NRPS	HC-toxin	Alternaria jesenskae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.79	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.79	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.78	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001825.4	0.78	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001135.5	0.77	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.76	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.76	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001133.4	0.76	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.75	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002075.3	0.75	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.74 out of 20.47

Confidence: medium

Additional weak hits (15):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.64 out of 28.14

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.91 out of 23.43

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.18 out of 23.43

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.93 out of 31.73

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.76 out of 27.55

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.11 out of 20.97

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.64 out of 21.84

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.52 out of 26.06

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.12 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 24.07 out of 32.74

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.49 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.97 out of 24.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 21.10 out of 29.25

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.94 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.40 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 344584 - >386024:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (ohmal) + (ohmal) + (X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 346034 - 449797:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (bLys - X - Gln) + (mal - D-Phe) + (fatty_acid - X - D-Ser - Ile - Val) + (pk) + (mal) + (mal) + (ohmal) + (ohmal) + (X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 346034 - 394634:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (ohmal) + (ohmal) + (X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 6, location 354693 - 424574:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (fatty_acid - X - D-Ser - Ile - Val) + (pk) + (mal) + (mal) + (ohmal) + (ohmal) + (X - X - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 7, location 387721 - 449797:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (pk) + (fatty_acid - X - D-Ser - Ile - Val) + (mal - D-Phe) + (bLys - X - Gln)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS22240: X - X - X

AMP-binding (512..913): X

nrpys: bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LENAFDASTLEGWLLAGGDVNGYGPTENTTFSAT
Nearest Stachelhaus code(s):: bOH-Val DALWLGGTFK (85% 8Å match); Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1584..1981): X

nrpys: Gln, Glu, Ile

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LALAFDASVQQVDCLAGGEFNVYGPTECTVDSTL
Nearest Stachelhaus code(s):: Gln or Glu DAQDLGVVDK (85% 8Å match); Ile DAQDLGVVDK (85% 8Å match); Gln DAQDLGVVDK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2643..2861): X

nrpys: Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LANAFDASTLEG----------------------
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

BLU09_RS22245: mal

PKS_AT (535..826): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 104.6
inactive: 70.7
Methoxymalonyl-CoA: 63.0
Methylmalonyl-CoA: 50.0
2-Methylbutyryl-CoA: 36.7
Ethylmalonyl-CoA: 33.7
Propionyl-CoA: 33.6
Benzoyl-CoA: 19.0
Isobutyryl-CoA: 13.4
CHC-CoA: 12.2
fatty_acid: 8.4
Acetyl-CoA: 8.2
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: -1.6

BLU09_RS22250: mal

PKS_AT (538..834): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; 2-Rhyd-Malonyl-CoA: 58.3%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 139.8
inactive: 92.8
Methylmalonyl-CoA: 76.1
Methoxymalonyl-CoA: 68.9
Ethylmalonyl-CoA: 50.7
2-Methylbutyryl-CoA: 50.5
Propionyl-CoA: 46.1
Benzoyl-CoA: 33.1
Isobutyryl-CoA: 33.0
CHC-CoA: 31.3
Acetyl-CoA: 24.9
3-Methylbutyryl-CoA: 15.9
trans-1,2-CPDA: 9.8
fatty_acid: 0.0

BLU09_RS22255: mal

PKS_AT (538..836): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 87.5%; Methylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 185.7
inactive: 132.6
Methoxymalonyl-CoA: 75.1
Methylmalonyl-CoA: 62.1
Propionyl-CoA: 48.6
Ethylmalonyl-CoA: 29.8
Isobutyryl-CoA: 28.5
CHC-CoA: 26.1
Benzoyl-CoA: 24.6
Acetyl-CoA: 24.0
2-Methylbutyryl-CoA: 24.0
fatty_acid: 13.5
trans-1,2-CPDA: 13.2
3-Methylbutyryl-CoA: 0.0

BLU09_RS22300: mal

PKS_AT (532..826): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 187.1
inactive: 101.8
Methoxymalonyl-CoA: 79.9
Methylmalonyl-CoA: 65.5
Propionyl-CoA: 45.7
Isobutyryl-CoA: 44.5
Ethylmalonyl-CoA: 44.3
2-Methylbutyryl-CoA: 35.4
Benzoyl-CoA: 28.8
CHC-CoA: 27.5
trans-1,2-CPDA: 24.0
fatty_acid: 22.1
Acetyl-CoA: 21.1
3-Methylbutyryl-CoA: 11.7

BLU09_RS22305: pk

PKS_AT (537..831): pk

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 62.5%; Malonyl-CoA: 54.2%; Ethylmalonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 138.0
inactive: 78.1
Methoxymalonyl-CoA: 74.3
Methylmalonyl-CoA: 69.2
Ethylmalonyl-CoA: 47.4
Propionyl-CoA: 46.8
2-Methylbutyryl-CoA: 34.2
Isobutyryl-CoA: 30.5
Benzoyl-CoA: 27.7
CHC-CoA: 20.8
Acetyl-CoA: 19.7
3-Methylbutyryl-CoA: 16.4
trans-1,2-CPDA: 12.3
fatty_acid: 11.5

BLU09_RS22320: fatty_acid - X - Ser - Ile - Val

Search NORINE for peptide: strict or relaxed

CAL_domain (38..487): fatty_acid

minowa: fatty_acid

Prediction, score:

fatty_acid: 27.3
AHBA: 17.3
NH2: 17.0
Acetyl-CoA: 0.7
shikimic_acid: 0.0

A-OX (1167..1931): X

No predictions made

AMP-binding (2568..2968): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFCSGEHNLYGPTEAAVDVTA
Nearest Stachelhaus code(s):: Ser DVWHFSLVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4045..4445): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: LETSFDAATFEGFLHFGGELHVYGPTENTTFSTW
Nearest Stachelhaus code(s):: Ile DAFFLGVTFK (94% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (5106..5502): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGSAFDAATFEGWLLAGGDVNGYGPTEGTTFTCC
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS22325: mal - X - Phe

Search NORINE for peptide: strict or relaxed

PKS_AT (532..829): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; Propionyl-CoA: 54.2%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 115.0
inactive: 56.2
Methoxymalonyl-CoA: 54.7
Methylmalonyl-CoA: 50.2
Ethylmalonyl-CoA: 28.7
Propionyl-CoA: 27.1
Isobutyryl-CoA: 26.5
CHC-CoA: 23.2
Benzoyl-CoA: 18.8
fatty_acid: 15.7
2-Methylbutyryl-CoA: 13.0
Acetyl-CoA: 11.6
trans-1,2-CPDA: 10.4
3-Methylbutyryl-CoA: 6.0

A-OX (1532..2155): X

No predictions made

AMP-binding (2757..3148): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: TTIAFDASVLESTLISAGELNGYGPTEASVCATL
Nearest Stachelhaus code(s):: Phe DAWTIAGVCK (59% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLU09_RS22330: bLys - X - Gln - X

Search NORINE for peptide: strict or relaxed

AMP-binding (494..899): bLys

nrpys: bLys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAEAWDTSAEEPNLVIGGELNTYGLTEVTAVATA
Nearest Stachelhaus code(s):: bLys DTENVGTAVK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1561..1957): X

nrpys: Val, bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGTAFDASTLEGWLLAGGDINGYGPTENTTFTST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (85% 8Å match); bOH-Val DALWLGGTFK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2623..3017): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HWFAFDASVPEAQLVSGGEINAYGPTETTVDATA
Nearest Stachelhaus code(s):: Arg DAESVGAVDK (74% 8Å match); Gln DAWQVGVVDK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (3679..3923): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGTAFDASTLEGWL--------------------
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNAJ01000013.1 - Region 1 - RiPP-like

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Location: 154,273 - 166,258 nt. (total: 11,986 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: esterase

BLU09_RS29060: putative esterase

smcogs: SMCOG1104: putative esterase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002115.3	0.41	ribosomal	streptamidine	Streptomyces albidoflavus J1074
BGC0001224.5	0.34	other (other)	reductasporine	uncultured bacterium
BGC0002482.2	0.29	other (other)	tjipanazole D, tjipanazole I, tjipanazole L, tjipanazole K, tjipanazole M	Symphyonema bifilamentata 97.28
BGC0001334.5	0.28	other (other)	caldoniamide A, cladoniamide B, cladoniamide C7, cladoniamide D, cladoniamide E, cladoniamide F, cladoniamide G	Streptomyces uncialis
BGC0001337.5	0.26	other (other)	lazarimide A, lazarimide B, lazarimide C	uncultured bacterium
BGC0000821.5	0.25	other (other)	rebeccamycin	Lechevalieria aerocolonigenes
BGC0001333.5	0.25	other (other)	BE-54017	uncultured bacterium AB1650
BGC0000611.5	0.24	ribosomal	siomycin A	Streptomyces sioyaensis
BGC0000448.5	0.23	NRPS	tomaymycin	Streptomyces achromogenes
BGC0001652.5	0.21	NRPS	limazepine C, limazepine D, limazepine E, limazepine F, limazepine A	Streptomyces sp. ICBB 8177

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002115.3	0.41	ribosomal	streptamidine	Streptomyces albidoflavus J1074
BGC0001224.5	0.34	other (other)	reductasporine	uncultured bacterium
BGC0002482.2	0.29	other (other)	tjipanazole D, tjipanazole I, tjipanazole L, tjipanazole K, tjipanazole M	Symphyonema bifilamentata 97.28
BGC0001334.5	0.28	other (other)	caldoniamide A, cladoniamide B, cladoniamide C7, cladoniamide D, cladoniamide E, cladoniamide F, cladoniamide G	Streptomyces uncialis
BGC0001337.5	0.26	other (other)	lazarimide A, lazarimide B, lazarimide C	uncultured bacterium
BGC0000821.5	0.25	other (other)	rebeccamycin	Lechevalieria aerocolonigenes
BGC0001333.5	0.25	other (other)	BE-54017	uncultured bacterium AB1650
BGC0000611.5	0.24	ribosomal	siomycin A	Streptomyces sioyaensis
BGC0000448.5	0.23	NRPS	tomaymycin	Streptomyces achromogenes
BGC0001652.5	0.21	NRPS	limazepine C, limazepine D, limazepine E, limazepine F, limazepine A	Streptomyces sp. ICBB 8177

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.28 out of 23.05

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.28 out of 19.52

Confidence: medium

Additional weak hits (1):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.52 out of 26.12

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
AfsR	Pleiotropic regulatory for antibiotic production

NZ_FNAJ01000013.1 - Region 2 - RiPP-like

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Location: 182,168 - 193,013 nt. (total: 10,846 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	RiPP-like	Similarity score	Type	Compound(s)	Organism
BGC0002665.2		0.29	ribosomal	3-thiaglutamate	Pseudomonas syringae pv. maculicola str. ES4326
BGC0001486.4		0.28	ribosomal	3-thiaglutamate	Pseudomonas syringae pv. maculicola str. ES4326

Reference	Aggregated	Similarity score	Type	Compound(s)	Organism
BGC0002665.2		0.29	ribosomal	3-thiaglutamate	Pseudomonas syringae pv. maculicola str. ES4326
BGC0001486.4		0.28	ribosomal	3-thiaglutamate	Pseudomonas syringae pv. maculicola str. ES4326

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (5):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.51 out of 29.38

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.83 out of 25.88

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.56 out of 29.38

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.95 out of 29.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.61 out of 29.25

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_FNAJ01000003.1 - Region 1 - NRPS-like,T1PKS

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Location: 10,107 - 61,941 nt. (total: 51,835 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acylase formyltransferase

BLU09_RS10450: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLU09_RS10460: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.60	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.59	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.59	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0000107.3	0.59	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001257.3	0.59	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001273.3	0.59	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.59	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001244.3	0.59	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.58	PKS	1-heptadecene	Cyanothece sp. PCC 7822

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0000107.3	0.63	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	0.63	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.63	PKS	1-heptadecene	Cyanothece sp. PCC 7822

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

Additional weak hits (12):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.56 out of 29.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.57 out of 31.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.47 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.89 out of 30.25

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.25 out of 36.54

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.11 out of 28.14

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.42 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.14 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.44 out of 31.73

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.52 out of 32.93

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.42 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.04 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 10106 - 61941:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 10106 - 56373:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 17264 - 61941:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS10450: mal

PKS_AT (539..836): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 58.3%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 142.1
inactive: 85.1
Methoxymalonyl-CoA: 73.4
Methylmalonyl-CoA: 67.0
Isobutyryl-CoA: 66.3
Propionyl-CoA: 48.9
Ethylmalonyl-CoA: 40.2
Benzoyl-CoA: 36.9
fatty_acid: 23.2
Acetyl-CoA: 19.3
3-Methylbutyryl-CoA: 18.0
CHC-CoA: 17.9
trans-1,2-CPDA: 8.4
2-Methylbutyryl-CoA: -1.0

BLU09_RS10460: X

A-OX (598..1375): X

No predictions made

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNAJ01000003.1 - Region 2 - thioamitides

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Location: 504,277 - 526,606 nt. (total: 22,330 nt) Show pHMM detection rules used

thioamitides: ((YcaO or TIGR03604) and TfuA)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

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Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

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Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001849.4	0.46	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0000625.5	0.34	ribosomal	thioviridamide	Streptomyces olivoviridis
BGC0002339.3	0.34	terpene	2-hydroxysorangiadenosine	Vitiosangium cumulatum
BGC0001430.4	0.27	NRPS, PKS	myxochromide S	Stigmatella aurantiaca Sg a15
BGC0001053.5	0.26	NRPS, PKS	tubulysin A	Archangium disciforme
BGC0001127.5	0.26	NRPS	jagaricin	Janthinobacterium agaricidamnosum
BGC0002326.2	0.25	NRPS	endopyrrole B, endopyrrole C, endopyrrole A	Mycetohabitans rhizoxinica
BGC0001473.4	0.23	ribosomal	Sch 18640	Micromonospora arborensis
BGC0000434.5	0.22	NRPS	SW-163C, UK-63598, SW-163E, SW-163F, SW-163G	Streptomyces sp. SNA15896
BGC0000119.5	0.20	PKS, saccharide	7-deoxypactamycin	Streptomyces pactum

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001849.4	0.46	ribosomal	thiovarsolin A, thiovarsolin B, thiovarsolin C, thiovarsolin D	Streptomyces varsoviensis
BGC0000625.5	0.34	ribosomal	thioviridamide	Streptomyces olivoviridis
BGC0002339.3	0.34	terpene	2-hydroxysorangiadenosine	Vitiosangium cumulatum
BGC0001430.4	0.27	NRPS, PKS	myxochromide S	Stigmatella aurantiaca Sg a15
BGC0001053.5	0.26	NRPS, PKS	tubulysin A	Archangium disciforme
BGC0001127.5	0.26	NRPS	jagaricin	Janthinobacterium agaricidamnosum
BGC0002326.2	0.25	NRPS	endopyrrole B, endopyrrole C, endopyrrole A	Mycetohabitans rhizoxinica
BGC0001473.4	0.23	ribosomal	Sch 18640	Micromonospora arborensis
BGC0000434.5	0.22	NRPS	SW-163C, UK-63598, SW-163E, SW-163F, SW-163G	Streptomyces sp. SNA15896
BGC0000119.5	0.20	PKS, saccharide	7-deoxypactamycin	Streptomyces pactum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.44 out of 23.79

Confidence: medium

Additional weak hits (5):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 26.33

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.91 out of 29.65

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.94 out of 32.93

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.00 out of 32.38

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.56 out of 26.85

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_FNAJ01000002.1 - Region 1 - T3PKS

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Location: 463,687 - 504,769 nt. (total: 41,083 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

BLU09_RS08730: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

BLU09_RS08745: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: methyltransferase

BLU09_RS08715: isoprenylcysteine carboxyl methyltransferase

smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

BLU09_RS08760: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

Ligases: ligase synthase

BLU09_RS08710: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

BLU09_RS08725: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001831.3	0.94	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0000282.3	0.75	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001164.3	0.58	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001310.5	0.54	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0001964.2	0.51	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0001962.3	0.50	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0002733.3	0.47	other (other)	sinapigladioside	Burkholderia gladioli
BGC0000916.5	0.45	other (other)	molybdenum cofactor	Staphylococcus carnosus
BGC0002332.2	0.44	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001831.3	0.94	PKS	alkylpyrone-407, alkylpyrone-393	Myxococcus xanthus DK 1622
BGC0000282.3	0.75	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001164.3	0.58	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.58	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001310.5	0.54	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0001964.2	0.51	PKS	alkylpyrone-407, alkylpyrone-393	Cystobacterineae bacterium
BGC0001962.3	0.50	PKS	hierridin B, hierridin C	Cyanobium sp. LEGE 06113
BGC0002733.3	0.47	other (other)	sinapigladioside	Burkholderia gladioli
BGC0000916.5	0.45	other (other)	molybdenum cofactor	Staphylococcus carnosus
BGC0002332.2	0.44	PKS	4-hydroxy-6-pentadecylpyran-2-one, 4-hydroxy-6-(2-oxoheptadecyl)pyran-2-one, Adipostatin A	Candidatus Entotheonella sp.

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.07 out of 20.47

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.73 out of 27.78

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.63 out of 28.19

Confidence: medium

Additional weak hits (18):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.87 out of 20.01

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.22 out of 26.12

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.18 out of 26.06

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.20 out of 30.11

Confidence: weak

CelR

Description: Cellobiose uptake repressor
Species: Streptomyces coelicolor
Score: 23.20 out of 30.11

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.80 out of 29.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 23.43 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 25.19 out of 32.74

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.78 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.79 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.44 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.92 out of 27.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.51 out of 21.84

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.13 out of 39.62

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.80 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.13 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.85 out of 20.01

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

BLU09_RS08725: NH2

CAL_domain (173..484): NH2

minowa: NH2

Prediction, score:

NH2: 99.0
Acetyl-CoA: 20.3
AHBA: 9.7
fatty_acid: 5.1
shikimic_acid: 0.0

TFBS definitions

Regulator	Description
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
VqsM	Regulator of quorum-sensing signalling systems
ZuR variant 1	Zinc-responsive repressor

NZ_FNAJ01000002.1 - Region 2 - HR-T2PKS,lanthipeptide-class-ii

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Location: 743,860 - 790,591 nt. (total: 46,732 nt) Show pHMM detection rules used

HR-T2PKS: (hr-t2pks-ksa and (t2fas or APE_KS2 or ketoacyl-synt))
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU09_RS09825: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

BLU09_RS09830: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Hydrolases: hydrolase

BLU09_RS09850: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

Lyases: cyclase

BLU09_RS09890: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Ligases: ligase synthase

BLU09_RS09815: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS09820: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS09840: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS09845: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS09930: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002274.2	0.52	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0002192.3	0.49	PKS	FR901512	fungal sp. No.14919
BGC0001258.3	0.45	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001257.3	0.45	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.45	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0000866.5	0.40	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0001197.5	0.40	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001723.5	0.39	PKS	oviedomycin	Streptomyces antibioticus
BGC0000231.5	0.37	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0000234.5	0.37	PKS	jadomycin	Streptomyces venezuelae ATCC 10712

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002274.2	0.59	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0002192.3	0.58	PKS	FR901512	fungal sp. No.14919
BGC0001258.3	0.56	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0001257.3	0.56	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002154.2	0.56	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0000866.5	0.53	other (other)	polyhydroxyalkanoate	Burkholderia sp. DSM 9242
BGC0001197.5	0.53	PKS	frankiamicin	Frankia sp. EAN1pec
BGC0001987.2	0.52	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis
BGC0000231.5	0.52	PKS	griseusin A, griseusin B	Streptomyces griseus
BGC0001723.5	0.51	PKS	oviedomycin	Streptomyces antibioticus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BLU09_RS09890

allorf_780201_780386

Leader:

MDMRRAPGGRRHKDCAGG

Core:

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMGRH

Core with dehydrations:

RERRDhaRGGLEQARARDhaCysPDhaAHGAVRPVPFDDhbAPVRRHVAMGRH

antiSMASH-DB 4.0 matches for allorf_780201_780386

100.0%

NZ_FNAJ01000002.1:

Lanthipeptides: allorf_780201_780386_lanthipeptide

1

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMGRH

43

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMGRH

1

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMGRH

43

97.7%

NZ_CP017173.1and 1 other:

NZ_CP113950.1 Lanthipeptides: allorf_8170940_8171125_lanthipeptide

Lanthipeptides: allorf_8000986_8001171_lanthipeptide

1

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMGRH

43

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAM RH

1

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMCRH

43

95.3%

NZ_CP017170.1:

Lanthipeptides: allorf_7967497_7967682_lanthipeptide

1

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMGRH

43

RERRSRGGL+QARARSCPSAHGAVRPVPFDTAPVRRHVAM RH

1

RERRSRGGLKQARARSCPSAHGAVRPVPFDTAPVRRHVAMCRH

43

with 7 more sequence matches

95.3%

NZ_CP017172.1:

Lanthipeptides: allorf_8124090_8124275_lanthipeptide

1

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMGRH

43

RERRSRGGLEQARARSCPSA GAVRPVPFDTAPVRRHVAM RH

1

RERRSRGGLEQARARSCPSAPGAVRPVPFDTAPVRRHVAMCRH

43

86.0%

NZ_CP017171.1:

Lanthipeptides: allorf_7652556_7652741_lanthipeptide

1

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAPVRRHVAMGRH

43

RERRSRGGLEQARARSCPS H AVRPVPFDT VRRHVAM H

1

RERRSRGGLEQARARSCPSVHAAVRPVPFDTGSVRRHVAMCPH

43

56.1%

NZ_CP017169.1:

Lanthipeptides: allorf_8041282_8041509_lanthipeptide

1

RERRSRGGLEQARARSCPSAHGAVRPVPFDTAP

33

RERRSRGGLEQARARSCPS HGAVRPVPFDTAP

1

RERRSRGGLEQARARSCPSGHGAVRPVPFDTAP

33

25.6%

NZ_CP017169.1:

Lanthipeptides: allorf_8041304_8041468_lanthipeptide

28

PFDTAPVRRHVAMGRH

43

P PVRRHVAM RH

20

PSTPPPVRRHVAMCRH

35

23.2%

NZ_FNPT01000006.1:

Lanthipeptides: allorf_214830_215027_lanthipeptide

7

GGLEQARARSCPSAHGAVRPVPFDTAPVR

35

GL+ ARARS G R P D A +R

16

AGLDDARARS-----GLRRSAPPDAACIR

39

18.6%

NZ_MKZR01000001.1:

Lanthipeptides: allorf_3001482_3001715_lanthipeptide

9

LEQARARSCPSAHGAVRPVPF

29

L A R C + AV+ PF

3

LRTAINRYCLNTESAVKQYPF

23

12.3%

NZ_BMJA01000007.1:

Lanthipeptides: allorf_031163_031396_lanthipeptide

13

RARSCPSAHGAVRPVPFDT

31

R+R C S + PF T

38

RSRMCVSKQSVLASFPFST

56

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.75 out of 23.79

Confidence: medium

Additional weak hits (2):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.99 out of 29.25

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.84 out of 25.88

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

Lanthipeptides

NRPS/PKS substrates

TFBS Finder

Core peptide predictions

allorf_780201_780386 - Class II

Cleavage pHMM score: -3.40

RODEO score: 0

Monoisotopic mass: 4714.5 Da

Molecular weight: 4717.3 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4735.3 Da N = 1; 4753.4 Da N = 2; 4771.4 Da N = 3

NRPS/PKS substrate predictions

BLU09_RS09930: X

AMP-binding (36..439): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HACVHAAAWGLDGGLIGGSVHAWGMTEMSPVGTM
Nearest Stachelhaus code(s):: Leu DALLLGAVVK (35% 8Å match); Phe DAGALGACAK (29% 8Å match)
Stachelhaus code match:: 60% (weak)

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_FNAJ01000005.1 - Region 1 - NRPS,T1PKS

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Location: 1 - 41,707 nt. (total: 41,707 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

BLU09_RS16225: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Ligases: ligase synthase

BLU09_RS16210: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU09_RS16215: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS16220: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001250.4	0.77	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.77	NRPS	epichloenin A	Epichloe festucae
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.72	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	0.69	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.68	NRPS	AbT1	Aureobasidium pullulans
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001133.4	0.68	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001250.4	0.71	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.71	NRPS	epichloenin A	Epichloe festucae
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	0.68	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.67	NRPS	AbT1	Aureobasidium pullulans
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001133.4	0.67	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.90 out of 30.22

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.29 out of 29.65

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.51 out of 35.63

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.08 out of 25.88

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 41707:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NH2 - X) + (mal - D-X) + (Ser)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS16210: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (467..871): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YAASADLGNTVPKLVVGGEYNHYGPTEATVGVLA
Nearest Stachelhaus code(s):: Ser DLTKVGHVGK (76% 8Å match); Asp DLTKVGHVGK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS16215: mal - X - X

PKS_AT (535..830): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 163.9
inactive: 82.8
Methoxymalonyl-CoA: 71.4
Methylmalonyl-CoA: 60.3
CHC-CoA: 38.6
Propionyl-CoA: 35.2
Ethylmalonyl-CoA: 35.1
Isobutyryl-CoA: 32.7
fatty_acid: 21.5
Benzoyl-CoA: 19.7
2-Methylbutyryl-CoA: 18.7
Acetyl-CoA: 11.1
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

A-OX (1500..2121): X

No predictions made

AMP-binding (2729..3122): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AARTFDASLAGWVSSVGGEFNEYGPTEATIWSTV
Nearest Stachelhaus code(s):: Trp DAAIIGEIWK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

BLU09_RS16220: NH2 - X

CAL_domain (49..497): NH2

minowa: NH2

Prediction, score:

NH2: 179.8
AHBA: 24.1
fatty_acid: 4.9
shikimic_acid: 0.0
Acetyl-CoA: -3.7

AMP-binding (1174..1568): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LSMAFDMSAWDLTLMVAAEYNGYGPTEVTVGATW
Nearest Stachelhaus code(s):: Kyn DAWTTTGVGK (65% 8Å match); Phe DAWTIAGVCK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description

NZ_FNAJ01000005.1 - Region 2 - NRPS,T1PKS

Download region SVG

Location: 106,512 - 175,540 nt. (total: 69,029 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: back translocase

BLU09_RS16680: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

Ligases: ligase synthase

BLU09_RS16655: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

BLU09_RS16660: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU09_RS16665: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001426.4	1.04	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001425.4	1.03	NRPS, PKS	myxochromide A	Myxococcus xanthus DK 1622
BGC0001427.4	1.02	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001423.4	0.85	NRPS, PKS	myxochromide C	Myxococcus hansupus
BGC0001428.4	0.82	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001421.4	0.81	NRPS, PKS	myxochromide B	Myxococcus sp.
BGC0001023.5	0.79	NRPS, PKS	myxochromide S1	Stigmatella aurantiaca
BGC0001424.4	0.77	NRPS, PKS	myxochromide C	Myxococcus virescens
BGC0000342.4	0.77	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001426.4	0.91	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001425.4	0.91	NRPS, PKS	myxochromide A	Myxococcus xanthus DK 1622
BGC0001427.4	0.90	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001423.4	0.84	NRPS, PKS	myxochromide C	Myxococcus hansupus
BGC0001421.4	0.83	NRPS, PKS	myxochromide B	Myxococcus sp.
BGC0001428.4	0.83	NRPS, PKS	myxochromide A	Myxococcus xanthus
BGC0001023.5	0.81	NRPS, PKS	myxochromide S1	Stigmatella aurantiaca
BGC0001424.4	0.80	NRPS, PKS	myxochromide C	Myxococcus virescens
BGC0001422.4	0.77	NRPS, PKS	myxochromide A	Myxococcus fulvus HW-1
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 24.36 out of 28.19

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (9):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.44 out of 26.85

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.76 out of 26.33

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.78 out of 26.12

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.82 out of 18.57

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.30 out of 27.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.79 out of 28.19

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.86 out of 24.78

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.79 out of 32.93

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.00 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 106511 - 175540:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (pk) + (NMe-Thr - D-Ala) + (Leu - Pro - Ala - Gln)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS16655: pk

PKS_AT (544..840): pk

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; CE-Malonyl-CoA: 62.5%; Methylmalonyl-CoA: 54.2%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 111.1
Malonyl-CoA: 101.1
Isobutyryl-CoA: 96.5
Methoxymalonyl-CoA: 92.6
Ethylmalonyl-CoA: 79.1
2-Methylbutyryl-CoA: 50.8
Propionyl-CoA: 48.3
Benzoyl-CoA: 45.8
CHC-CoA: 41.6
trans-1,2-CPDA: 34.3
Acetyl-CoA: 31.5
3-Methylbutyryl-CoA: 24.1
inactive: 23.2
fatty_acid: 21.2

BLU09_RS16660: Thr - Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (508..912): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LTSHFDFSVWEGNQIFGGEINMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2007..2397): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LASAFDVSVWDMVLITAGEINAYGPTETTVIATV
Nearest Stachelhaus code(s):: Ala DVWVIAAVIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS16665: Leu - Pro - Ala - Gln

Search NORINE for peptide: strict or relaxed

AMP-binding (482..868): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDAAVWEPFLLTGGDVNNYGPTETTVVATS
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1517..1912): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQAFDVSFQESFLITAGDHNHYGPTESHVVTAH
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2566..2956): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VMQSFDVSVWEMVLTSAGEVNAYGPTEVSIISTL
Nearest Stachelhaus code(s):: Ala DVWVTAAIIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3609..4000): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HWMTFDASVWEAQLCVGGEWNLYGPTEATIDSLA
Nearest Stachelhaus code(s):: Gln DAWQCGLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
ZuR variant 1	Zinc-responsive repressor

NZ_FNAJ01000005.1 - Region 3 - RiPP-like

Download region SVG

Location: 229,626 - 241,278 nt. (total: 11,653 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000043.5	0.39	PKS	curacin A	Moorea producens 19L
BGC0000527.5	0.34	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000547.5	0.34	ribosomal	salivaricin 9	Streptococcus salivarius
BGC0000556.5	0.33	ribosomal	streptin	Streptococcus pyogenes
BGC0002007.2	0.33	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0000515.4	0.33	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000506.4	0.33	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0002346.3	0.32	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000557.5	0.32	ribosomal	streptococcin A-FF22	Streptococcus pyogenes
BGC0001789.3	0.32	ribosomal	suicin 90-1330	Streptococcus suis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000043.5	0.39	PKS	curacin A	Moorea producens 19L
BGC0000527.5	0.34	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0000547.5	0.34	ribosomal	salivaricin 9	Streptococcus salivarius
BGC0000556.5	0.33	ribosomal	streptin	Streptococcus pyogenes
BGC0002007.2	0.33	terpene	atolypene A, atolypene B	Amycolatopsis tolypomycina
BGC0000515.4	0.33	ribosomal	geobacillin I	Geobacillus thermodenitrificans NG80-2
BGC0000506.4	0.33	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0002346.3	0.32	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000557.5	0.32	ribosomal	streptococcin A-FF22	Streptococcus pyogenes
BGC0001789.3	0.32	ribosomal	suicin 90-1330	Streptococcus suis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (3):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.00 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.01 out of 26.06

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.38 out of 32.93

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NZ_FNAJ01000005.1 - Region 4 - NRPS,T1PKS

Download region SVG

Location: 365,397 - 442,291 nt. (total: 76,895 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

BLU09_RS17745: monooxygenase FAD-binding

smcogs: SMCOG1246: monooxygenase FAD-binding

BLU09_RS17755: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: acylase

BLU09_RS17695: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLU09_RS17720: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase

BLU09_RS17685: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

BLU09_RS17715: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU09_RS17725: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS17730: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS17735: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS17740: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

BLU09_RS17760: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.95	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.95	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.95	PKS	asperlactone	Aspergillus ochraceus
BGC0000342.4	0.73	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001276.3	0.69	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001758.4	0.68	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001275.3	0.77	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.77	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.77	PKS	asperlactone	Aspergillus ochraceus
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001276.3	0.67	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.55 out of 18.46

Confidence: strong

Additional weak hits (11):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.82 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.94 out of 29.38

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.07 out of 21.55

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.79 out of 27.04

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.35 out of 27.55

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.39 out of 36.54

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.08 out of 28.19

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 19.14 out of 22.40

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.62 out of 26.12

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.55 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 365396 - 442291:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr - ccmal) + (ccmal) + (ccmal) + (Asn) + (ccmal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS17695: mal

PKS_AT (563..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 174.6
inactive: 136.8
Methoxymalonyl-CoA: 55.7
Methylmalonyl-CoA: 53.3
Propionyl-CoA: 38.6
Benzoyl-CoA: 31.4
Isobutyryl-CoA: 30.3
Acetyl-CoA: 26.9
Ethylmalonyl-CoA: 21.1
trans-1,2-CPDA: 13.9
2-Methylbutyryl-CoA: 12.8
CHC-CoA: 10.5
fatty_acid: 0.0
3-Methylbutyryl-CoA: 0.0

BLU09_RS17715: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (536..940): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWSGFDGTLTSAKMVLGGEINEYGPTEAAVACCF
Nearest Stachelhaus code(s):: Asn DATKVGEVGK (74% 8Å match); OH-Asn or Asn DATKVGEVGK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

BLU09_RS17720: mal

PKS_AT (558..851): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Propionyl-CoA: 62.5%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 175.9
inactive: 95.4
Methoxymalonyl-CoA: 90.6
Methylmalonyl-CoA: 85.1
Ethylmalonyl-CoA: 59.9
Propionyl-CoA: 57.4
2-Methylbutyryl-CoA: 46.4
Isobutyryl-CoA: 43.5
Acetyl-CoA: 29.1
fatty_acid: 25.8
CHC-CoA: 25.8
Benzoyl-CoA: 21.6
trans-1,2-CPDA: 17.1
3-Methylbutyryl-CoA: 9.6

BLU09_RS17725: mal

PKS_AT (567..857): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 95.8%; inactive: 91.7%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 190.5
inactive: 132.3
Methoxymalonyl-CoA: 76.7
Methylmalonyl-CoA: 56.5
Isobutyryl-CoA: 37.3
Propionyl-CoA: 35.4
2-Methylbutyryl-CoA: 27.8
Ethylmalonyl-CoA: 21.0
fatty_acid: 20.8
Acetyl-CoA: 20.8
Benzoyl-CoA: 20.3
3-Methylbutyryl-CoA: 19.1
CHC-CoA: 12.8
trans-1,2-CPDA: 11.3

BLU09_RS17730: Thr - mal

Search NORINE for peptide: strict or relaxed

AMP-binding (542..941): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: GWTSHDFSVYDGSTMLGGDMSVGGPTETSLWNIS
Nearest Stachelhaus code(s):: Thr DFYSMGVLWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1681..1975): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 62.5%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 133.5
inactive: 89.1
Methoxymalonyl-CoA: 57.0
Methylmalonyl-CoA: 54.5
Benzoyl-CoA: 26.0
Propionyl-CoA: 24.0
Isobutyryl-CoA: 20.9
Ethylmalonyl-CoA: 18.8
trans-1,2-CPDA: 17.9
2-Methylbutyryl-CoA: 13.4
Acetyl-CoA: 10.2
CHC-CoA: 9.6
3-Methylbutyryl-CoA: 8.9
fatty_acid: 0.0

BLU09_RS17760: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (8..409): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FWCAFDLSVLDAYSLFAGEFNLYGPTETNVCTFH
Nearest Stachelhaus code(s):: Pro DLLYLALVCK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FNAJ01000005.1 - Region 5 - NRPS,T1PKS

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Location: 514,539 - 584,690 nt. (total: 70,152 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU09_RS18340: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acylase acyltransferase glycosyltransferase

BLU09_RS18255: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

BLU09_RS18260: polyketide synthase associated protein papA3

smcogs: SMCOG1209: polyketide synthase associated protein papA3

BLU09_RS18355: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: esterase

BLU09_RS18200: putative esterase

smcogs: SMCOG1104: putative esterase

BLU09_RS18210: thioesterase superfamily protein

smcogs: SMCOG1144: thioesterase superfamily protein

Ligases: ligase

BLU09_RS18265: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU09_RS18270: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU09_RS18280: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.95	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.90	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001758.4	0.89	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.88	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.85	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.82	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.81	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002071.2	0.80	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001166.5	0.80	NRPS	HC-toxin	Alternaria jesenskae
BGC0001825.4	0.80	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.78	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.78	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.78	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128.4	0.77	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.77	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.76	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002075.3	0.75	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0002286.3	0.75	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.75	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002071.2	0.73	NRPS	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.15 out of 20.97

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 16.86 out of 18.57

Confidence: medium

Additional weak hits (2):

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.88 out of 19.26

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.59 out of 26.06

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 5, location 514538 - 584690:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal - X) + (Gly) + (Thr - X - D-Phe - Orn) + (Orn)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS18255: mal - X

PKS_AT (649..944): mal

ATSignature: Malonyl-CoA, inactive

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 91.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 183.8
inactive: 140.4
Methoxymalonyl-CoA: 89.9
Methylmalonyl-CoA: 70.6
Isobutyryl-CoA: 56.4
2-Methylbutyryl-CoA: 56.0
Propionyl-CoA: 55.8
Ethylmalonyl-CoA: 45.6
CHC-CoA: 30.4
trans-1,2-CPDA: 27.8
Acetyl-CoA: 22.5
Benzoyl-CoA: 20.5
3-Methylbutyryl-CoA: 17.9
fatty_acid: 12.3

AMP-binding (2133..2537): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HDMAFDASLEQPNFIIGGDLNAYGPTETLITATT
Nearest Stachelhaus code(s):: Arg DAEDIGAITK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)

BLU09_RS18260: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (538..928): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FSMTFDISVLELQALCGGEWNMYGPTETTIWSTT
Nearest Stachelhaus code(s):: Gly DILQLGMIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS18265: Thr - X - Phe - Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (470..879): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LATHFDFSVWEGNQVFGGEINMYGITETTVHVTY
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1550..1952): X

nrpys: Gln, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWATFDATITSTKLVVGGEINEYGPTETVVGCST
Nearest Stachelhaus code(s):: Gln or Asn DATKVGEVGK (85% 8Å match); Asn DATKVGEVGK (82% 8Å match); OH-Asn or Asn DATKVGEVGK (79% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2615..3005): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TTAAFDASVCESTLVSAGEINAYGPTEATICASV
Nearest Stachelhaus code(s):: Phe DAWTVAAICK (62% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (4127..4535): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: AGWAFDVFAGDREFVAGSDINSYGVSEATIDSTW
Nearest Stachelhaus code(s):: Orn DVGEVGSIDK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS18270: Orn

Search NORINE for peptide: strict or relaxed

AMP-binding (515..919): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: AAWAFDVFSGDREFIVGSDVSSYGLSEATIDSTY
Nearest Stachelhaus code(s):: Orn DVGEIGSIDK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

BLU09_RS18280: NH2

CAL_domain (41..486): NH2

minowa: NH2

Prediction, score:

NH2: 32.8
Acetyl-CoA: 31.4
fatty_acid: 21.0
AHBA: 2.9
shikimic_acid: 0.0

TFBS definitions

Regulator	Description
CcpA	Catabolite control protein A
ToxT	Regulator of MSHA biosynthesis

NZ_FNAJ01000001.1 - Region 1 - NRPS,NRPS-like

Download region SVG

Location: 51,874 - 117,064 nt. (total: 65,191 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

BLU09_RS00385: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: acyltransferase aminotransferase methyltransferase

BLU09_RS00345: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

BLU09_RS00355: aminotransferase

smcogs: SMCOG1019: aminotransferase

BLU09_RS00360: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

BLU09_RS00375: acyltransferase

smcogs: SMCOG1141: acyltransferase

Lyases: dehydratase

BLU09_RS00380: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Isomerases: isomerase

BLU09_RS00405: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

BLU09_RS00320: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

BLU09_RS00340: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

BLU09_RS00410: carbamoyl phosphate synthase small subunit

smcogs: SMCOG1249: carbamoyl phosphate synthase small subunit

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002274.2	0.56	NRPS	aspulvinone H, aspulvinone B1	Aspergillus terreus NIH2624
BGC0002277.2	0.55	NRPS	atromentin	Suillus grevillei
BGC0002193.2	0.55	other (other)	phenguignardic acid	Aspergillus terreus NIH2624
BGC0001833.4	0.44	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001164.3	0.44	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0002135.2	0.43	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.41	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.41	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.41	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.40	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001833.4	0.75	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0000307.4	0.68	NRPS	AbT1	Aureobasidium pullulans

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.22 out of 18.46

Confidence: strong

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 20.76 out of 21.84

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.23 out of 23.79

Confidence: medium

Additional weak hits (16):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.33 out of 30.25

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.34 out of 27.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.26 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.42 out of 30.25

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 20.01 out of 27.04

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.87 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.15 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.34 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.01 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.25 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.87 out of 26.06

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.01 out of 29.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.71 out of 29.38

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.04 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.79 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.17 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 51873 - 117064:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X - X - X - Asn - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 51873 - 108688:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X - X - X - Asn - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 72906 - 117064:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (X - X - X - Asn - X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS00320: X - X - X - Asn - X

Search NORINE for peptide: strict or relaxed

AMP-binding (513..919): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HGMAFDISIWQGEMVLGGEADAYGPTESSDVASL
Nearest Stachelhaus code(s):: Dpr DIWEVTADDK (62% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (1576..1979): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HGMAFDISIWQGELLTIGEVNAYGPAECADTATL
Nearest Stachelhaus code(s):: Dpr DIWELTADDK (68% 8Å match); Dab DIWELTADDK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

AMP-binding (2638..3023): X

nrpys: Glu, Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: CAFGFDIHLEEFVIVTGGEVNTYGPTETSINVAA
Nearest Stachelhaus code(s):: N6-ohLys or Lys DIESIGTIVK (56% 8Å match); Hpg DIWHVGTIIK (53% 8Å match); Arg DVENVGAINK (50% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (3677..4080): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: YWASFDLTVTSLKLVIGGEINEYGPTETVVGCCV
Nearest Stachelhaus code(s):: Asn DLTKVGEVGK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4741..5145): X

nrpys: Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HALAFDAAAEDPSLAPGGDLNVYGPTECTITSAT
Nearest Stachelhaus code(s):: Arg DAEDIGAITK (59% 8Å match); Ahp DVENAGVVTK (59% 8Å match); Lys DAESVGTIIK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

BLU09_RS00340: X

AMP-binding (169..577): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MGMA--TSYPHAWAASGGECPAWGMAETSSFFSM
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
MexT	Global virulence regulator
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FNAJ01000001.1 - Region 2 - NRPS-like

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Location: 164,443 - 208,855 nt. (total: 44,413 nt) Show pHMM detection rules used

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU09_RS00755: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: transferase

BLU09_RS00735: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Hydrolases: glucanase

BLU09_RS00690: Pullulanase, type I

smcogs: SMCOG1297: Pullulanase, type I

Lyases: cyclase dehydratase

BLU09_RS00705: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

BLU09_RS00725: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

BLU09_RS00730: diguanylate cyclase

smcogs: SMCOG1025: diguanylate cyclase

Ligases: ligase

BLU09_RS00720: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000871.4	0.96	other (nucleoside)	VEPE, AEPE, TG-1	Myxococcus xanthus DK 1622
BGC0002135.2	0.59	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001641.4	0.58	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001285.5	0.47	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0002474.2	0.46	NRPS	agrobactin	Agrobacterium tumefaciens
BGC0001168.5	0.44	NRPS	Livipeptin	Streptomyces lividans 1326
BGC0002711.2	0.43	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0000898.5	0.43	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000826.5	0.42	other (other)	staurosporine	Streptomyces clavuligerus ATCC 27064

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000871.4	0.96	other (nucleoside)	VEPE, AEPE, TG-1	Myxococcus xanthus DK 1622
BGC0002135.2	0.59	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001641.4	0.58	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001285.5	0.47	other (fatty acid)	pseudopyronine A, pseudopyronine B	Pseudomonas putida
BGC0002474.2	0.46	NRPS	agrobactin	Agrobacterium tumefaciens
BGC0001168.5	0.44	NRPS	Livipeptin	Streptomyces lividans 1326
BGC0002711.2	0.43	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0000898.5	0.43	saccharide	desosamine, pikromycin, neomethymycin, narbomycin	Streptomyces venezuelae
BGC0000826.5	0.42	other (other)	staurosporine	Streptomyces clavuligerus ATCC 27064

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (7):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.22 out of 25.75

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.00 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.24 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.72 out of 26.06

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.09 out of 30.98

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.48 out of 32.38

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 24.79 out of 32.93

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 164442 - 208855:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS00720: X

AMP-binding (568..998): X

nrpys: Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LKLLFEFAAG-TVG--------------ALMATH
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator

NZ_FNAJ01000001.1 - Region 3 - terpene

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Location: 891,375 - 919,320 nt. (total: 27,946 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

BLU09_RS03685: Halogenation

MITE: MITE0000024Halogenation (identity: 64.50%)
smcogs: SMCOG1222: dehydrogenase

BLU09_RS03695: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: dehydrogenase

BLU09_RS03685: Halogenation

MITE: MITE0000024Halogenation (identity: 64.50%)
smcogs: SMCOG1222: dehydrogenase

Hydrolases: esterase

BLU09_RS03715: enterobactin/ferric enterobactin esterase

smcogs: SMCOG1098: enterobactin/ferric enterobactin esterase

Lyases: dehydratase

BLU09_RS03710: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthetase

BLU09_RS03700: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000648.3	0.93	terpene	carotenoid	Myxococcus xanthus
BGC0000647.3	0.76	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000664.5	0.58	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.57	terpene	isorenieratene	Streptomyces argillaceus
BGC0001227.3	0.55	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000644.3	0.53	terpene	carotenoid	Dietzia sp. CQ4
BGC0000633.3	0.53	terpene	carotenoid	Streptomyces avermitilis
BGC0000649.3	0.53	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000656.5	0.51	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000646.5	0.49	terpene	β-carotein	uncultured bacterium

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000648.3	0.93	terpene	carotenoid	Myxococcus xanthus
BGC0000647.3	0.76	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000664.5	0.58	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.57	terpene	isorenieratene	Streptomyces argillaceus
BGC0001227.3	0.55	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000644.3	0.53	terpene	carotenoid	Dietzia sp. CQ4
BGC0000633.3	0.53	terpene	carotenoid	Streptomyces avermitilis
BGC0000649.3	0.53	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000656.5	0.51	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000646.5	0.49	terpene	β-carotein	uncultured bacterium

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
tetraterpene	C40 carotenoid		C40	unknown	unknown

Genes with terpene domains:

BLU09_RS03665: Lycopene_cycl

Domain 1 (6 - 99): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

BLU09_RS03670: Lycopene_cycl

Domain 1 (1 - 91): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

BLU09_RS03690: PT_phytoene_like

Domain 1 (13 - 315): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → PSPP; FPP → squalene; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

BLU09_RS03700: PT_FPPS_like

Domain 1 (56 - 359): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (2):

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 16.57 out of 26.12

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.88 out of 30.97

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description

NZ_FNAJ01000014.1 - Region 1 - terpene

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Location: 15,995 - 38,259 nt. (total: 22,265 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: reductase

BLU09_RS29445: hydroxymethylglutaryl-CoA reductase

smcogs: SMCOG1187: hydroxymethylglutaryl-CoA reductase

Transferases: acyltransferase

BLU09_RS29470: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

Lyases: cyclase

BLU09_RS29430: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002708.2	0.74	terpene	(+)-δ-cadinol	Coniophora puteana RWD-64-598 SS2
BGC0000657.5	0.58	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000286.3	0.38	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0000642.3	0.34	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000640.3	0.33	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000634.3	0.32	terpene	carotenoid	Brevundimonas sp. SD212
BGC0001080.5	0.31	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000934.4	0.30	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0001902.3	0.27	NRPS, PKS	bengamide	Myxococcus virescens

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002708.2	0.74	terpene	(+)-δ-cadinol	Coniophora puteana RWD-64-598 SS2
BGC0000657.5	0.58	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000286.3	0.38	PKS	viguiepinol	Streptomyces sp. KO-3988
BGC0000642.3	0.34	terpene	carotenoid	Enterobacteriaceae bacterium DC413
BGC0000640.3	0.33	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0000634.3	0.32	terpene	carotenoid	Brevundimonas sp. SD212
BGC0001080.5	0.31	other (phenazine)	endophenazine A, endophenazine B	Streptomyces anulatus
BGC0000934.4	0.30	other (phenazine)	5-acetyl-5,10-dihydrophenazine-1-carboxylic acid, 5-(2-hydroxyacetyl)-5,10-dihydrophenazine-1-carboxylic acid, endophenazine A1, endophenazine F, endophenazine G	Kitasatospora sp. HKI 714
BGC0001902.3	0.27	NRPS, PKS	bengamide	Myxococcus virescens

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

BLU09_RS29430: T1TS + T1TS

Domain 1 (2 - 337): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (350 - 717): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 26.55 out of 29.72

Confidence: strong

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 20.38 out of 25.88

Confidence: medium

Additional weak hits (2):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.23 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.36 out of 26.06

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
NtrC	Nitrogen regulatory protein C
PsrA	Oleic acid responsive regulator

NZ_FNAJ01000052.1 - Region 1 - NRPS

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Location: 1 - 1,233 nt. (total: 1,233 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

BLU09_RS38010: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000342.4	0.64	NRPS	enniatin	Fusarium equiseti
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001240.4	0.63	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.66	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.64	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000342.4	0.64	NRPS	enniatin	Fusarium equiseti
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001240.4	0.63	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001132.4	0.63	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.63	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NZ_FNAJ01000024.1 - Region 1 - lanthipeptide-class-ii

Download region SVG

Location: 25,453 - 48,617 nt. (total: 23,165 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase

BLU09_RS35455: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000554.4	0.57	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002703.2	0.49	ribosomal	archalan α	Halorussus salinus
BGC0001210.5	0.48	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0001555.4	0.47	ribosomal	colicin V	Escherichia coli chi7122
BGC0002698.2	0.47	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000590.5	0.47	ribosomal	microcin N	Escherichia coli
BGC0001579.4	0.47	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000517.4	0.46	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000516.4	0.46	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0000504.5	0.45	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000554.4	0.57	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0002703.2	0.49	ribosomal	archalan α	Halorussus salinus
BGC0001210.5	0.48	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0001555.4	0.47	ribosomal	colicin V	Escherichia coli chi7122
BGC0002698.2	0.47	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0000590.5	0.47	ribosomal	microcin N	Escherichia coli
BGC0001579.4	0.47	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000517.4	0.46	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125
BGC0000516.4	0.46	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0000504.5	0.45	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for BLU09_RS35455

BLU09_RS35460

Leader:

MSQKKDHILRAWRDPEYFNSLSSEERAALPANPAAELELGDDLLEVITGG

Core:

DFCLPGQSSAQCTPCPPRHCL

Core with dehydrations:

DFCysLPGQDhaDhaAQCysDhbPCysPPRHCysL

antiSMASH-DB 4.0 matches for BLU09_RS35460

100.0%

NC_008095.1and 2 others:

NZ_CP017171.1 Lanthipeptides: BHS06_RS13080_lanthipeptideNZ_FNAJ01000024.1 Lanthipeptides: BLU09_RS35460_lanthipeptide

Lanthipeptides: MXAN_RS13835_lanthipeptide

1

DFCLPGQSSAQCTPCPPRHCL

21

DFCLPGQSSAQCTPCPPRHCL

1

DFCLPGQSSAQCTPCPPRHCL

21

90.5%

NZ_CP017169.1and 5 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13865_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14010_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13995_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14530_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14525_lanthipeptide

Lanthipeptides: BHS04_RS14460_lanthipeptide

1

DFCLPGQSSAQCTPCPPRHCL

21

DFCLPGQSS QCTPCPPRHC

1

DFCLPGQSSPQCTPCPPRHCF

21

76.2%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13860_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14005_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13990_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14520_lanthipeptide

Lanthipeptides: BHS04_RS14455_lanthipeptide

3

CLPGQSSAQCTPCPPRHCL

21

C PGQSSA CTPCPP HCL

3

CKPGQSSAICTPCPPYHCL

21

with 13 more sequence matches

Only the first 10 are displayed here.

71.4%

NZ_CP068048.1:

Lanthipeptides: JKA73_RS13975_lanthipeptide

1

DFCLPGQSSAQCTPCPPRHCL

21

DFC GQSS CTPCPP HC

1

DFCPTGQSSPYCTPCPPLHCY

21

57.1%

Lanthipeptides: A176_RS20100_lanthipeptide

5

PGQSSAQCTPCPPRHCL

21

P Q SA CTPCPP HC+

4

PEQCSALCTPCPPYHCV

20

47.6%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13855_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14000_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13985_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14515_lanthipeptide

Lanthipeptides: BHS04_RS14450_lanthipeptide

8

SSAQCTPCPPRHCL

21

S CTPCPP HCL

7

GSYYCTPCPPYHCL

20

47.6%

NZ_CP059736.1:

Lanthipeptides: SG35_RS31610_lanthipeptide

10

AQCTPCPPRHCL

21

A C+PCPPRHCL

1

AICSPCPPRHCL

12

42.9%

NC_015711.1and 1 other:

NZ_CP022203.1 Lanthipeptides: MYMAC_RS14145_lanthipeptide

Lanthipeptides: LILAB_RS21660_lanthipeptide

8

SSAQCTPCPPRHCL

21

S CTPCPP CL

7

GSYHCTPCPPYVCL

20

42.9%

NZ_CP022098.1:

Lanthipeptides: CYFUS_RS36740_lanthipeptide

8

SSAQCTPCPPRHC

20

+SA CTPCPP++C

6

TSAYCTPCPPKYC

18

42.9%

NZ_CP069396.1and 1 other:

NZ_CP069396.1 Lanthipeptides: JRI60_RS18040_lanthipeptide

Lanthipeptides: JRI60_RS18040_lanthipeptide

8

SSAQCTPCPPRHCL

21

+SA CTPCPP++C

6

TSAYCTPCPPKYCY

19

38.1%

NC_008095.1and 3 others:

NZ_CP017171.1 Lanthipeptides: BHS06_RS13075_lanthipeptideNZ_CP068048.1 Lanthipeptides: JKA73_RS13970_lanthipeptideNZ_FNAJ01000024.1 Lanthipeptides: BLU09_RS35465_lanthipeptide

Lanthipeptides: MXAN_RS13830_lanthipeptide

9

SAQCTPCPPRHCL

21

S CTPCPP C+

8

SYYCTPCPPYVCM

20

38.1%

Lanthipeptides: A176_RS20105_lanthipeptide

9

SAQCTPCPPRHCL

21

S CTPCPP C

8

SPYCTPCPPIECY

20

38.1%

BGC0002130.3 (QKI29070.1):

Lanthipeptides: A176_RS20110_lanthipeptide

9

SAQCTPCPPRHCL

21

S CTPCPP C+

8

SYYCTPCPPFVCM

20

MIBiG 4.0 matches for BLU09_RS35460

21.7%

Lanthipeptide: cacaoidin

8

SSAQCT

13

SSA CT

1

SSAPCT

6

BLU09_RS35465

Leader:

MSKKQHILRAWRDPEYFNSLSSEERAALPANPAAELELSDEVLESISGA

Core:

DSCEQFGSYYCTPCPPYVCM

Core with dehydrations:

DDhaCysEQFGDhaYYCysDhbPCysPPYVCysM

antiSMASH-DB 4.0 matches for BLU09_RS35465

100.0%

NC_008095.1and 3 others:

NZ_CP017171.1 Lanthipeptides: BHS06_RS13075_lanthipeptideNZ_CP068048.1 Lanthipeptides: JKA73_RS13970_lanthipeptideNZ_FNAJ01000024.1 Lanthipeptides: BLU09_RS35465_lanthipeptide

Lanthipeptides: MXAN_RS13830_lanthipeptide

1

DSCEQFGSYYCTPCPPYVCM

20

DSCEQFGSYYCTPCPPYVCM

1

DSCEQFGSYYCTPCPPYVCM

20

95.0%

Lanthipeptides: A176_RS20110_lanthipeptide

1

DSCEQFGSYYCTPCPPYVCM

20

DSCEQFGSYYCTPCPP+VCM

1

DSCEQFGSYYCTPCPPFVCM

20

85.0%

NC_015711.1and 1 other:

NZ_CP022203.1 Lanthipeptides: MYMAC_RS14145_lanthipeptide

Lanthipeptides: LILAB_RS21660_lanthipeptide

1

DSCEQFGSYYCTPCPPYVCM

20

DSCE+FGSY+CTPCPPYVC+

1

DSCERFGSYHCTPCPPYVCL

20

with 13 more sequence matches

Only the first 10 are displayed here.

85.0%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13855_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14000_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13985_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14515_lanthipeptide

Lanthipeptides: BHS04_RS14450_lanthipeptide

1

DSCEQFGSYYCTPCPPYVCM

20

D+CEQFGSYYCTPCPPY C+

1

DTCEQFGSYYCTPCPPYHCL

20

70.0%

Lanthipeptides: A176_RS20105_lanthipeptide

1

DSCEQFGSYYCTPCPPYVC

19

DSCE GS YCTPCPP C

1

DSCETIGSPYCTPCPPIEC

19

45.0%

BGC0001155.5 (AGY49587.1):

Lanthipeptides: A176_RS20100_lanthipeptide

8

SYYCTPCPPYVCM

20

S CTPCPPY C+

8

SALCTPCPPYHCV

20

45.0%

NZ_CP022098.1and 2 others:

NZ_CP069396.1 Lanthipeptides: JRI60_RS18040_lanthipeptideNZ_CP069396.1 Lanthipeptides: JRI60_RS18040_lanthipeptide

Lanthipeptides: CYFUS_RS36740_lanthipeptide

8

SYYCTPCPPYVC

19

S YCTPCPP C

7

SAYCTPCPPKYC

18

42.9%

NZ_CP017169.1and 4 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13860_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14005_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13990_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14520_lanthipeptide

Lanthipeptides: BHS04_RS14455_lanthipeptide

8

SYYCTPCPPYVCM

20

S CTPCPPY C+

9

SAICTPCPPYHCL

21

42.9%

NZ_CP068048.1:

Lanthipeptides: JKA73_RS13975_lanthipeptide

7

GSYYCTPCPPYVC

19

S YCTPCPP C

8

SSPYCTPCPPLHC

20

38.1%

NC_008095.1and 2 others:

NZ_CP017171.1 Lanthipeptides: BHS06_RS13080_lanthipeptideNZ_FNAJ01000024.1 Lanthipeptides: BLU09_RS35460_lanthipeptide

Lanthipeptides: MXAN_RS13835_lanthipeptide

8

SYYCTPCPPYVCM

20

S CTPCPP C+

9

SAQCTPCPPRHCL

21

38.1%

NZ_CP017169.1and 5 others:

NZ_CP017170.1 Lanthipeptides: BHS05_RS13865_lanthipeptideNZ_CP017172.1 Lanthipeptides: BHS07_RS14010_lanthipeptideNZ_CP017173.1 Lanthipeptides: BHS08_RS13995_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14530_lanthipeptideNZ_CP113950.1 Lanthipeptides: OZ403_RS14525_lanthipeptide

Lanthipeptides: BHS04_RS14460_lanthipeptide

7

GSYYCTPCPPYVCM

20

S CTPCPP C

8

SSPQCTPCPPRHCF

21

36.4%

NZ_CP040916.1:

Lassopeptides: FH965_RS40555_lassopeptide

3

CEQFGSYYCTPCPPY

17

E GSYY PPY

1

GEALGSYYEGGFPPY

15

30.0%

NZ_CP059736.1:

Lanthipeptides: SG35_RS31610_lanthipeptide

11

CTPCPPYVCM

20

C+PCPP C+

3

CSPCPPRHCL

12

MIBiG 4.0 matches for BLU09_RS35465

15.0%

Thiopeptide: GE2270

10

YCTPCPP

16

+C C P

8

FCCSCSP

14

allorf_35060_35209

Leader:

MKPCLGKLQRQRPTARATVAVA

Core:

CVVQIPPTFPLGLHARRTRAPSIDRQR

Core with dehydrations:

CysVVQIPPDhbFPLGLHARRDhbRAPDhaIDRQR

antiSMASH-DB 4.0 matches for allorf_35060_35209

100.0%

NZ_FNAJ01000024.1:

Lanthipeptides: allorf_35060_35209_lanthipeptide

1

CVVQIPPTFPLGLHARRTRAPSIDRQR

27

CVVQIPPTFPLGLHARRTRAPSIDRQR

1

CVVQIPPTFPLGLHARRTRAPSIDRQR

27

13.7%

NZ_BNBY01000037.1:

Lanthipeptides: IE332_RS36065_lanthipeptide

9

FPLGLHARRTRAPSID

24

PLG H T P D

8

LPLGWHREGTEGPKQD

23

allorf_35242_35448

Leader:

MRAAADLSCCVVLDLRVNSARSVSVAA

Core:

RAQRANRDGVLAPGIASSCLCVISTRPLVSWAKPGELASLE

Core with dehydrations:

RAQRANRDGVLAPGIADhaDhaCysLCysVIDhaDhbRPLVDhaWAKPGELADhaLE

antiSMASH-DB 4.0 matches for allorf_35242_35448

100.0%

NZ_FNAJ01000024.1:

Lanthipeptides: allorf_35242_35448_lanthipeptide

1

RAQRANRDGVLAPGIASSCLCVISTRPLVSWAKPGELASLE

41

RAQRANRDGVLAPGIASSCLCVISTRPLVSWAKPGELASLE

1

RAQRANRDGVLAPGIASSCLCVISTRPLVSWAKPGELASLE

41

24.4%

NZ_CP048887.1:

Lanthipeptides: BMG_RS29390_lanthipeptide

5

ANRDGVLAPGIASSCLCV

22

AN G P IASS +CV

1

ANLQGEPIPTIASSAVCV

18

19.5%

NC_017030.1:

Lanthipeptides: allorf_07530736_07530897_lanthipeptide

7

RDGVLAPGIASSCL

20

R GVL P IA C

9

RRGVLRPAIARPCF

22

with 1 more sequence matches

14.6%

NC_008095.1:

Lanthipeptides: allorf_7874093_7874236_lanthipeptide

8

DGVLAPGIASSCLCV

22

+GV++ G+ S+ CV

8

NGVMSEGVTSTTSCV

22

allorf_47932_48033

Leader:

MVLGSSAGC

Core:

SSGDALPTCCSMSTRRFAVSEGES

Core with dehydrations:

DhaDhaGDALPDhbCysCysDhaMDhaDhbRRFAVDhaEGEDha

antiSMASH-DB 4.0 matches for allorf_47932_48033

100.0%

NZ_FNAJ01000024.1:

Lanthipeptides: allorf_47932_48033_lanthipeptide

1

SSGDALPTCCSMSTRRFAVSEGES

24

SSGDALPTCCSMSTRRFAVSEGES

1

SSGDALPTCCSMSTRRFAVSEGES

24

39.3%

NZ_CP017599.1:

Lanthipeptides: allorf_3513174_3513389_lanthipeptide

5

ALPTCCSMSTRRFAVSEGES

24

ALPTC TR AV +G S

5

ALPTC----TRTIAVCQGCS

20

23.5%

NZ_CP073318.1:

Lanthipeptides: allorf_01122276_01122440_lanthipeptide

6

LPTCCSMSTRRFAVSEG

22

LP C S R VS+G

18

LPACAGNSRRSLTVSQG

34

with 3 more sequence matches

20.9%

NZ_PUPX01000001.1:

Lanthipeptides: allorf_1091992_1092165_lanthipeptide

2

SGDALPTCCSMSTRRFA

18

+GD LPT C S R A

26

AGDQLPTGCGCSCVRDA

42

20.8%

NZ_JANAVO010000003.1:

Lanthipeptides: NLX83_RS21760_lanthipeptide

9

CCSMSTRR

16

CC+++TRR

8

CCTVTTRR

15

16.7%

NZ_JNJB01000019.1:

Lanthipeptides: Q369_RS0119815_lanthipeptide

1

SSGDALPTCCSM

12

+ GD P+CC +

8

AGGDNGPSCCGI

19

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.88 out of 20.47

Confidence: medium

Additional weak hits (2):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.90 out of 30.22

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

BLU09_RS35460 - Class II

Cleavage pHMM score: -2.80

RODEO score: 0

Monoisotopic mass: 2205.0 Da

Molecular weight: 2206.5 Da

Number of bridges: 3

BLU09_RS35465 - Class II

Cleavage pHMM score: -2.00

RODEO score: 0

Monoisotopic mass: 2237.8 Da

Molecular weight: 2239.5 Da

Number of bridges: 3

allorf_35060_35209 - Class II

Cleavage pHMM score: -5.90

RODEO score: 0

Monoisotopic mass: 3029.7 Da

Molecular weight: 3031.5 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3049.6 Da N = 1; 3067.6 Da N = 2

allorf_35242_35448 - Class II

Cleavage pHMM score: -6.30

RODEO score: 0

Monoisotopic mass: 4183.3 Da

Molecular weight: 4185.8 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 4203.8 Da N = 1; 4221.8 Da N = 2; 4239.9 Da N = 3; 4257.9 Da N = 4

allorf_47932_48033 - Class II

Cleavage pHMM score: -6.80

RODEO score: 0

Monoisotopic mass: 2333.1 Da

Molecular weight: 2334.5 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2352.6 Da N = 1; 2370.6 Da N = 2; 2388.6 Da N = 3; 2406.6 Da N = 4; 2424.6 Da N = 5; 2442.7 Da N = 6

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NZ_FNAJ01000043.1 - Region 1 - NRPS

Download region SVG

Location: 1 - 3,822 nt. (total: 3,822 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

BLU09_RS37925: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.66	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0002135.2	0.65	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001240.4	0.64	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.66	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.66	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0002135.2	0.65	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001240.4	0.64	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0000342.4	0.63	NRPS	enniatin	Fusarium equiseti

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 3822:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS37925: X

AMP-binding (124..521): X

nrpys: Val, bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGTAFDASTLEGWLLAGGDINGYGPTENTTFSST
Nearest Stachelhaus code(s):: Val DALWLGGTFK (82% 8Å match); bOH-Val DALWLGGTFK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

NZ_FNAJ01000038.1 - Region 1 - NRPS

Download region SVG

Location: 1 - 10,255 nt. (total: 10,255 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: methyltransferase

BLU09_RS37810: methyltransferase

smcogs: SMCOG1089: methyltransferase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.71	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.71	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.66	NRPS	AbT1	Aureobasidium pullulans
BGC0002286.3	0.66	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 10255:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS37810: X - X

AMP-binding (1171..1568): X

nrpys: bOH-Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LGSAFDASTLEGWLLAGGDVNGYGPTEGTTFTAC
Nearest Stachelhaus code(s):: bOH-Val DALWLGGTFK (97% 8Å match); Val DALWLGGTFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2228..2625): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LGSAFDVSSIEGVLLMGGETNGYGPTECSTMATR
Nearest Stachelhaus code(s):: Ala DVFWLGGTFK (59% 8Å match)
Stachelhaus code match:: 70% (weak)

NZ_FNAJ01000041.1 - Region 1 - NRPS

Download region SVG

Location: 1 - 5,863 nt. (total: 5,863 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

BLU09_RS37915: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.65	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.65	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001132.4	0.65	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001825.4	0.65	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0001128.4	0.65	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 5863:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

BLU09_RS37915: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (761..1165): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: YAASADLGNTVAKVVVGGEHNHYGPTETTVGVLA
Nearest Stachelhaus code(s):: Ser DLTKVGHVGK (79% 8Å match); Asp DLTKVGHVGK (76% 8Å match)
Stachelhaus code match:: 100% (strong)