LOCUS       NZ_FNAJ01000053         1226 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Myxococcus virescens strain DSM 2260, whole genome shotgun sequence.
ACCESSION   NZ_FNAJ01000053
VERSION     NZ_FNAJ01000053.1
KEYWORDS    WGS; RefSeq.
SOURCE      Myxococcus virescens
  ORGANISM  Myxococcus virescens
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Myxococcus.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900101905.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 02:08:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,594
            CDSs (total)                      :: 7,524
            Genes (coding)                    :: 7,456
            CDSs (with protein)               :: 7,456
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 15 of 68
            Pseudo Genes (incomplete)         :: 65 of 68
            Pseudo Genes (internal stop)      :: 7 of 68
            Pseudo Genes (multiple problems)  :: 17 of 68
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:03:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1226
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNAJ01000053.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     CDS_motif       complement(<1..29)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38015_0006"
                     /evalue="5.20E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="BLU09_RS38015"
                     /protein_end="409"
                     /protein_start="399"
                     /score="15.8"
                     /tool="antismash"
                     /translation="QYADYAVWQR"
     aSDomain        complement(<1..542)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLU09_RS38015_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="4.70E-69"
                     /label="BLU09_RS38015_Condensation_LCL.1"
                     /locus_tag="BLU09_RS38015"
                     /protein_end="409"
                     /protein_start="228"
                     /score="224.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVSDG"
                     /tool="antismash"
                     /translation="GALPLSFAQQRLWFLDQLEPGSAFYNVPVAVRLTGVLDIAALRRS
                     FDELVRRHESLRTTFRAQDGMPVQIVSDTASTRLEVLARGTPDDGEGGLETKRLVEREA
                     LRPFNLETGPLLRAALLKEGEEAHVLVLVMHHIVSDGWSMGVLVRELVSLYEAYSEGRE
                     SPLAELEVQYADYAVWQR"
     source          1..1226
                     /db_xref="taxon:83456"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0070493_153"
                     /organism="Myxococcus virescens"
                     /strain="DSM 2260"
                     /type_material="type strain of Myxococcus virescens"
     gene            complement(<1..>1226)
                     /locus_tag="BLU09_RS38015"
                     /old_locus_tag="SAMN04488504_1531"
     region          1..1226
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1226
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1226
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:1226](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..1226)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             complement(<1..1226)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (141-209). E-value: 1.8e-30. Score:
                     96.9. Matches aSDomain: nrpspksdomains_BLU09_RS38015_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (228-409). E-value:
                     4.7e-69. Score: 224.6. Matches aSDomain:
                     nrpspksdomains_BLU09_RS38015_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940245.1"
                     /locus_tag="BLU09_RS38015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_1531"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_143043295.1"
                     /sec_met_domain="AMP-binding (E-value: 3.6e-24, bitscore:
                     71.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.6e-21, bitscore:
                     62.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.4e-48, bitscore:
                     151.5, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLYRTGDVVRWLGNGTLEFLGRADTQVKVRGYRIELGEVEAAL
                     AQHSGVNEAVVVAREDGGEGKRLVAYVTAREAASLDAGALRSHVKQRLPEYMVPSAYVV
                     LESLPLTPNGKVDRKALPVPEAQGSKAAHFEAPRTVTEQKLASIFAEVLNVERVSVDED
                     FFELGGHSLLATQLVSRVRESFQVELPLRDVFESPTVEKLALRLGHAQVGGLMPQAPPL
                     MRAPRQGALPLSFAQQRLWFLDQLEPGSAFYNVPVAVRLTGVLDIAALRRSFDELVRRH
                     ESLRTTFRAQDGMPVQIVSDTASTRLEVLARGTPDDGEGGLETKRLVEREALRPFNLET
                     GPLLRAALLKEGEEAHVLVLVMHHIVSDGWSMGVLVRELVSLYEAYSEGRESPLAELEV
                     QYADYAVWQR"
     CDS_motif       complement(78..143)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38015_0005"
                     /evalue="6.30E-14"
                     /label="C3_LCL_132-143"
                     /locus_tag="BLU09_RS38015"
                     /protein_end="383"
                     /protein_start="361"
                     /score="44.2"
                     /tool="antismash"
                     /translation="MHHIVSDGWSMGVLVRELVSLY"
     CDS_motif       complement(171..203)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38015_0004"
                     /evalue="6.50E+00"
                     /label="Cy2"
                     /locus_tag="BLU09_RS38015"
                     /protein_end="352"
                     /protein_start="341"
                     /score="-0.1"
                     /tool="antismash"
                     /translation="PLLRAALLKEG"
     CDS_motif       complement(360..476)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38015_0003"
                     /evalue="6.90E-22"
                     /label="C2_LCL_024-062"
                     /locus_tag="BLU09_RS38015"
                     /protein_end="289"
                     /protein_start="250"
                     /score="69.9"
                     /tool="antismash"
                     /translation="AFYNVPVAVRLTGVLDIAALRRSFDELVRRHESLRTTFR"
     CDS_motif       complement(495..527)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38015_0002"
                     /evalue="5.20E-04"
                     /label="C1_LCL_004-017"
                     /locus_tag="BLU09_RS38015"
                     /protein_end="244"
                     /protein_start="233"
                     /score="12.3"
                     /tool="antismash"
                     /translation="SFAQQRLWFLD"
     aSDomain        complement(600..803)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLU09_RS38015_PCP.1"
                     /evalue="1.80E-30"
                     /label="BLU09_RS38015_PCP.1"
                     /locus_tag="BLU09_RS38015"
                     /protein_end="209"
                     /protein_start="141"
                     /score="96.9"
                     /tool="antismash"
                     /translation="EQKLASIFAEVLNVERVSVDEDFFELGGHSLLATQLVSRVRESFQ
                     VELPLRDVFESPTVEKLALRLGH"
     CDS_motif       complement(1101..1166)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38015_0001"
                     /evalue="8.20E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="BLU09_RS38015"
                     /protein_end="42"
                     /protein_start="20"
                     /score="40.1"
                     /tool="antismash"
                     /translation="FLGRADTQVKVRGYRIELGEVE"
ORIGIN
        1 cgctgccaca ccgcgtagtc cgcgtactgc acctccagct ccgccagcgg tgactcgcgc
       61 ccctccgagt acgcctcgta cagggacacc agctccctca ccagcacgcc catcgaccag
      121 ccgtccgaca cgatgtggtg catcaccaac accagcacgt gcgcttcttc cccctccttc
      181 agcagcgccg ccctcagcag cggccctgtc tccagattga acggacgaag cgcctcgcgc
      241 tccacgaggc gttttgtctc cagccctcct tcaccgtcgt ccggagttcc gcgcgccaag
      301 acttccaacc gggtcgatgc agtgtcggag acaatctgga caggcatccc gtcctgagca
      361 cggaaggtgg ttcgcaacga ttcatgtcgg cggaccaact catcgaagct acggcgcaac
      421 gctgcaatgt cgaggacgcc tgtgagcctc actgcgactg gcacgttgta gaaggcgctg
      481 ccaggctcca gttgatcgag gaaccacagt cgctgctgag cgaacgatag cggcaatgcc
      541 ccctggcgcg gtgcccgcat cagtggtggc gcctggggca tcaagccccc cacctgtgcg
      601 tgtcctagcc ggagggccag tttctccacc gtcggcgatt cgaaaacgtc cctcaggggg
      661 agttccacct gaaaggactc gcggacgcgc gagaccagct gcgtggccag cagcgagtgg
      721 ccacccagct cgaagaagtc ttcgtcgacg ctgactcgct cgacgttcag cacctcggcg
      781 aagatggacg ccagcttctg ctcagtcacc gtgcgaggcg cctcgaagtg cgcagccttc
      841 gagccttgtg cctctggaac gggcagcgcc ttgcggtcca cctttccgtt cggcgtcagc
      901 ggcagtgact ccagcaccac gtacgctgac ggcaccatgt actccggtag cctctgcttc
      961 acgtgacttc gcagtgcacc cgcgtccagc gaggcagcct cccgtgccgt cacgtaggcc
     1021 accaaccgct ttccttcgcc tccatcctcc cgcgccacca ccacggcttc gttgacgcca
     1081 ctgtgctggg ccagcgctgc ttccacttca cccagctcga tgcggtagcc gcgcaccttc
     1141 acctgcgtgt ccgctcggcc caggaactcc aacgtcccgt tccccagcca ccgcaccacg
     1201 tcccccgtgc ggtacagccg ctctcc
//
