LOCUS       NZ_FNAJ01000052         1233 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Myxococcus virescens strain DSM 2260, whole genome shotgun sequence.
ACCESSION   NZ_FNAJ01000052
VERSION     NZ_FNAJ01000052.1
KEYWORDS    WGS; RefSeq.
SOURCE      Myxococcus virescens
  ORGANISM  Myxococcus virescens
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Myxococcus.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900101905.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 02:08:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,594
            CDSs (total)                      :: 7,524
            Genes (coding)                    :: 7,456
            CDSs (with protein)               :: 7,456
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 15 of 68
            Pseudo Genes (incomplete)         :: 65 of 68
            Pseudo Genes (internal stop)      :: 7 of 68
            Pseudo Genes (multiple problems)  :: 17 of 68
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:03:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1233
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNAJ01000052.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1233
                     /db_xref="taxon:83456"
                     /mol_type="genomic DNA"
                     /note="contig: Ga0070493_152"
                     /organism="Myxococcus virescens"
                     /strain="DSM 2260"
                     /type_material="type strain of Myxococcus virescens"
     gene            <1..>1233
                     /locus_tag="BLU09_RS38010"
                     /old_locus_tag="SAMN04488504_1521"
     region          1..1233
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..1233
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1233
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>1233](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>1233
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>1233
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (146-215). E-value: 6.2e-29. Score:
                     92.0. Matches aSDomain: nrpspksdomains_BLU09_RS38010_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (234-411). E-value:
                     2e-66. Score: 216.0. Matches aSDomain:
                     nrpspksdomains_BLU09_RS38010_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940245.1"
                     /locus_tag="BLU09_RS38010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_1521"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_143043294.1"
                     /sec_met_domain="AMP-binding (E-value: 5.4e-23, bitscore:
                     67.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2e-22, bitscore:
                     65.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 4.5e-49, bitscore:
                     153.2, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPFGEGERLYRTGDVVRWLGNGTLEFLGRADTQVKVRGYRIELGE
                     VEAALAQYGGVNEAVVVAREDGGEGKRLVAYVTAQEGASLDTGALRSHVKQRLPEYMVP
                     SAYVVLESLPLTPNGKVDRKALPAPDAQGPKTAQFEAPRTATEEKLASIFAEVLNVERV
                     SVDEDFFELGGHSLLATQLVSRVREELQVELPLRDIFETSTVGKLAERIDASRGTTAAA
                     VRAPPLTRVSRSDALPLSFAQQRLWFLDQLEPGSPFYNVPSVVKLTGRLQTTALEASFS
                     ELVRRHESLRTTFRVSGGEPVQVIATDPLQPFQQLDFSEPEAERESRVRRWIEEEIQRP
                     FNLETGPLLRATLLKEGEEAHVLVLVMHHIVSDGWSMGVLVRELVALYEAYSQGRESPL
                     AELEVQYADYAV"
     CDS_motif       76..141
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38010_0001"
                     /evalue="8.20E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="BLU09_RS38010"
                     /protein_end="47"
                     /protein_start="25"
                     /score="40.1"
                     /tool="antismash"
                     /translation="FLGRADTQVKVRGYRIELGEVE"
     aSDomain        439..645
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLU09_RS38010_PCP.1"
                     /evalue="6.20E-29"
                     /label="BLU09_RS38010_PCP.1"
                     /locus_tag="BLU09_RS38010"
                     /protein_end="215"
                     /protein_start="146"
                     /score="92.0"
                     /tool="antismash"
                     /translation="EEKLASIFAEVLNVERVSVDEDFFELGGHSLLATQLVSRVREELQ
                     VELPLRDIFETSTVGKLAERIDAS"
     aSDomain        703..>1233
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLU09_RS38010_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="2.00E-66"
                     /label="BLU09_RS38010_Condensation_LCL.1"
                     /locus_tag="BLU09_RS38010"
                     /protein_end="411"
                     /protein_start="234"
                     /score="216.0"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVSDG"
                     /tool="antismash"
                     /translation="DALPLSFAQQRLWFLDQLEPGSPFYNVPSVVKLTGRLQTTALEAS
                     FSELVRRHESLRTTFRVSGGEPVQVIATDPLQPFQQLDFSEPEAERESRVRRWIEEEIQ
                     RPFNLETGPLLRATLLKEGEEAHVLVLVMHHIVSDGWSMGVLVRELVALYEAYSQGRES
                     PLAELEVQYADYAV"
     CDS_motif       718..750
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38010_0002"
                     /evalue="5.20E-04"
                     /label="C1_LCL_004-017"
                     /locus_tag="BLU09_RS38010"
                     /protein_end="250"
                     /protein_start="239"
                     /score="12.3"
                     /tool="antismash"
                     /translation="SFAQQRLWFLD"
     CDS_motif       769..885
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38010_0003"
                     /evalue="4.00E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="BLU09_RS38010"
                     /protein_end="295"
                     /protein_start="256"
                     /score="57.8"
                     /tool="antismash"
                     /translation="PFYNVPSVVKLTGRLQTTALEASFSELVRRHESLRTTFR"
     CDS_motif       1039..1071
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38010_0004"
                     /evalue="6.40E+00"
                     /label="Cy2"
                     /locus_tag="BLU09_RS38010"
                     /protein_end="357"
                     /protein_start="346"
                     /score="-0.1"
                     /tool="antismash"
                     /translation="PLLRATLLKEG"
     CDS_motif       1099..1164
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BLU09_RS38010_0005"
                     /evalue="2.20E-14"
                     /label="C3_LCL_132-143"
                     /locus_tag="BLU09_RS38010"
                     /protein_end="388"
                     /protein_start="366"
                     /score="45.6"
                     /tool="antismash"
                     /translation="MHHIVSDGWSMGVLVRELVALY"
ORIGIN
        1 agcccattcg gcgaaggaga gcggctgtac cgcacggggg acgtggtgcg gtggctgggg
       61 aacgggacgt tggagttcct gggccgagcg gacacacagg tgaaggtgcg tgggtaccgc
      121 atcgagttgg gtgaagtgga agcagcgctg gcccagtacg gtggcgtcaa cgaagccgtg
      181 gtggtggcgc gggaggatgg gggcgaaggc aagcggttgg tggcctacgt gacggcgcag
      241 gaaggtgcct cgctggacac gggggcactg cgaagccacg tgaagcagcg actgcccgag
      301 tacatggtgc cgtcagcata tgtggtgctg gagtcgctgc cgctgacgcc gaacggaaag
      361 gtggaccgca aggcgctgcc cgccccggat gcccaggggc cgaagacggc acagttcgag
      421 gcgcctcgca cggcgactga ggagaagctg gcgtccatct tcgccgaggt gctgaacgtc
      481 gagcgcgtca gcgtcgacga agacttcttc gagttgggcg gacactcgct gctggcaacg
      541 cagttggtgt cgcgagtgag ggaagaactc caggtagagc tcccgctgcg agacatcttc
      601 gaaacttcca cagtggggaa gctggccgaa cggattgacg catcccgcgg gacgacagcg
      661 gcggcggttc gagcaccacc cctgacgcgc gtttctcgga gcgatgcgct accgctgtca
      721 ttcgcgcagc agcggctgtg gttcctcgac cagttggagc caggcagtcc gttctacaac
      781 gtcccctccg tggtgaagtt gactgggcgt ctgcaaacga ccgcgttgga ggccagcttc
      841 agtgaactcg tgcggcggca cgagtcattg cgcacgacgt tccgtgtcag tggcggtgaa
      901 cctgtgcagg tcatcgcaac ggatccgctt cagcctttcc agcagctcga cttcagcgaa
      961 ccggaagcgg agcgcgaatc cagggtgcgg cgctggatcg aagaggagat tcagcgcccg
     1021 ttcaatctgg agacgggccc gttgctgagg gcgacgctac tgaaggaggg ggaagaagcg
     1081 cacgtgctgg tgctggtgat gcaccacatc gtgtcggacg ggtggtcgat gggcgtgctg
     1141 gtgagggagt tggtggctct gtacgaggcg tactcgcagg ggcgcgagtc accgctggcg
     1201 gagctggagg tgcagtacgc ggactacgcg gtg
//
