LOCUS       NZ_FNAJ01000013        11986 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Myxococcus virescens strain DSM 2260, whole genome shotgun sequence.
ACCESSION   NZ_FNAJ01000013
VERSION     NZ_FNAJ01000013.1
KEYWORDS    WGS; RefSeq.
SOURCE      Myxococcus virescens
  ORGANISM  Myxococcus virescens
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Myxococcus.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900101905.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 02:08:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,594
            CDSs (total)                      :: 7,524
            Genes (coding)                    :: 7,456
            CDSs (with protein)               :: 7,456
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 15 of 68
            Pseudo Genes (incomplete)         :: 65 of 68
            Pseudo Genes (internal stop)      :: 7 of 68
            Pseudo Genes (multiple problems)  :: 17 of 68
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:03:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 154272
            Orig. end                         :: 166258
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNAJ01000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11986
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11986
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11986
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6986](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6986)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(138..149)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.28"
                     /tool="antismash"
     gene            complement(887..1258)
                     /locus_tag="BLU09_RS29025"
                     /old_locus_tag="SAMN04488504_113108"
     CDS             complement(887..1258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478693.1"
                     /locus_tag="BLU09_RS29025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_113108"
                     /product="hypothetical protein"
                     /protein_id="WP_090493269.1"
                     /transl_table=11
                     /translation="MEAAQKQLEQVTGYARGRVKERLDFQRGLLVSVLEDMAEGLSSAT
                     RRAPSPASQRMLETGERMFRGASSRLDAQSVEAFLDRTGQFVRQRPRLLLTGLLGAGLL
                     AGRMFRASGAGDVEREAES"
     misc_feature    1353..1368
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     gene            1471..2142
                     /locus_tag="BLU09_RS29030"
                     /old_locus_tag="SAMN04488504_113109"
     CDS             1471..2142
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554906.1"
                     /locus_tag="BLU09_RS29030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_113109"
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="WP_244172095.1"
                     /transl_table=11
                     /translation="MKPEQEKTLHEEAVATVDRLVLKHPELKKELEQVEGYAVFPSLGR
                     ASLVLGGSYGHGEVFEKGKPIGFATLSQLTLGVQVGGQTLSELIFFHDRDALEAFKGGK
                     VAFAANASAVLVKAAASGTINYAKCVAHAYSRGGMLLEASLGGQKFTFIPQSGASERGN
                     GGLMQRFRRQDASEENGADDTGQRPRLQASQPPPALTTGLTAAALLLTRIIKAQASARH
                     E"
     gene            2126..3385
                     /locus_tag="BLU09_RS39535"
                     /old_locus_tag="SAMN04488504_113110"
     CDS             2126..3385
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLU09_RS39535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN04488504_113110"
                     /product="hypothetical protein"
                     /protein_id="WP_244172096.1"
                     /transl_table=11
                     /translation="MLATNEASLRDKLASQGHRALKLARKELSVHRRLHGDARAALHRL
                     EARSPELAQARARAYAYAVFPSLGQGSAVLGGTWGMGEVFRQERLVGYAALVQLTLGVQ
                     LGGQTASEVVLFEDQEAFQRFKHGGTGLALNAAATLVKAGAARARGPAGSTTVALATDG
                     GMWAGVALGAQRAFVVPAVLTRSSTLRRVLSSIPSLTRGTPAPNEGETRESAMAKAQTT
                     KSGRKRLGKLTAAWKRRKAHDEEKTGPKKVAVHTREAMQRVSTRGRETLEHLRDRVPSG
                     DRLRERAGQARTWAADQLGEHAVAIGLTTLAAGVAGAALLPVSNRERRALSSVSHKVQG
                     LTHSLGAAPQVARLTRSVSDAVARVRRGDGAATEEEKPRAPRPKKVAATRGKKAAGRPA
                     AAAKTSRRPRKGSPPPQASK"
     gene            complement(3560..4969)
                     /locus_tag="BLU09_RS29045"
                     /old_locus_tag="SAMN04488504_113111"
     CDS             complement(3560..4969)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554908.1"
                     /locus_tag="BLU09_RS29045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_113111"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_090493166.1"
                     /transl_table=11
                     /translation="MSERVIILGAGLAGLAAAHALVLAGRDVVVLEARSRVGGRVLTLR
                     QPFVDGMYAEAGAKYVLSGHSTVLGLARRLGLELDPLPVPHTSALRPFHLRGRRILAPF
                     GDDTGLPHVLRADEQQLGLSGLYRRYVSLLVEAVGDPFHESWPGPGATHLDGLTLREAL
                     AERGASSAAIDAVSLGRFDLVGDGIDASSALGVLRRELLTVGRGGSSAYTLRGGSDRLP
                     VALAEALGGRIQFGWAAKRIEQEQGTVRVYCRGSTGEHRTFTAERLVCAIPFSVLRGVE
                     LVPAFSADKLRAIRELEHTSVVRTFIQYRRRLWTEWGWSSGWLTDLPIMHVLDAAPTQP
                     PPSGILEAYRVGPCARAAGLLSEEERIAMAVRGLSQFAPELSSEVQCGTSYAWDLDPWA
                     RGAFAWFRPRQLTQWGRALTTREGRVHFAGDQTSPMPGWMEGALLSGQRAALEILSGAS
                     GACMAEGLHGA"
     gene            complement(5001..6986)
                     /locus_tag="BLU09_RS29050"
                     /old_locus_tag="SAMN04488504_113112"
     CDS             complement(5001..6986)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="BLU09_RS29050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_113112"
                     /product="YcaO-like family protein"
                     /protein_id="WP_090493168.1"
                     /sec_met_domain="YcaO (E-value: 1.1e-51, bitscore: 169.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGVQSGVVLLEMARGSSILQTRRALMEIRGLPFALLVSILGDVRR
                     GGSAAVVRALVKAGVDPTGARELGARLEEEGALGRGAPRWMDEQALRKELGITGRLRLG
                     PLRTADLSENSGALIQLSATRWRSAGSGVCIACGLLWEVQSADAPDEAAAVANALPRRA
                     VIALGTADLTALRKAMKPHSRDARAWTEGAYGRPGTRRIRSRPAAGCACSANRGAANHI
                     GKLEGSRMAGAALGRLGIATREATVRGRRGAPPIVTLSWGAATLRLRKRGRPVVSGHFL
                     AQVGVGASLEERRLTARAEAIERASSLLRVPDVCATPARALEQPFLPMLDWDLYTPEQY
                     ASPGFPYAPVTQDTPLDWVRATDAETGEQLLVPAAFTTSERLVGPRFLCASSNGVATHT
                     SDDAALRSALLEVVERDALQLAWYRGQGARRLDMATLDIDEGVSRHFKGSCWTLHCSYL
                     PGRAGLHVIALIAEATGEGAFPKGGTLLAAAAAGDPAVAVHRALREMRMLTEAVALRRE
                     LAIDFEALRRPPVLENYWLIDSLLDITLLYLNPAMRSAVDLLLEGPPVALPRVRVPDTA
                     GELVTRFRQHGLRTLVINLTLAAASPFRTCQALVLGTQPLAFCPSLLRLGSGLLPRRLP
                     PRNPQLPSSPLELRRGLLNPYLLPLA"
     misc_feature    complement(5118..5120)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            7537..8808
                     /locus_tag="BLU09_RS29055"
                     /old_locus_tag="SAMN04488504_113114"
     CDS             7537..8808
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615348.1"
                     /locus_tag="BLU09_RS29055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_113114"
                     /product="hypothetical protein"
                     /protein_id="WP_167371178.1"
                     /sec_met_domain="PF00561 (E-value: 3.1e-06, bitscore: 20.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKLGLSFIVLFACTSAFASPPPRIETEAESLASTPTPFAVLREF
                     VTEDIYHYRFDIRVGTAPNAIVRVHRVVRERAPWRPRSPAHAIMLLHGDFANFVTNFLP
                     TLGIPASSAPGLAPYLAARDIDVWGVDRRWTLPSANGDISDLGEMGVAQELGDITVALT
                     FARAARLSGDRDKGRIVLGGFSHGGELAYAYAAANGRHVSAVAVLDAYYDIAPEHAELR
                     ELACANAAAGREALAQGVTDSDNSFFITLGELARSAPQAQSPVFSSYTNRGALLAMNGL
                     AYLLAPYTPLYHLSAPILDANGDVTALRESPEDSVSAWFASAPPHQSLRETVELDEIWC
                     GTTPRPELLNIRVPLFYLGAAGAFGDYGLYSTTRVSSTDVTALVVRRFGPERVAEDFGH
                     GDLLYAADAPTLAWQPLATWLLSH"
     gene            complement(8924..9925)
                     /locus_tag="BLU09_RS29060"
                     /old_locus_tag="SAMN04488504_113115"
     CDS             complement(8924..9925)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1104: putative esterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478689.1"
                     /locus_tag="BLU09_RS29060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_113115"
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="WP_090493170.1"
                     /transl_table=11
                     /translation="MGVLLFALFLGVPGDAEASDASLAPNFYSGHGITVHAVRHITDRL
                     IDVEISTPLIAPHAVYDPRHHVRVLLPTGYYNNPGVRYPAVYLLHGGGGANSAQWVEFG
                     AAYAITENMPVITIMPDGGKVGWYTNWVFPRGVNQAWEEFHLNQLIPWVDQNLRTLAYK
                     RGRAIAGLSMGGFGAISYAARRPDLFAYAASFSGALDLGDAAIRATITEEGTRWLQNPD
                     GAFGSPFWPFDQAWNMNNPIPRAASLRGVAVALYAGSGIWDGDVLERVPGASTDRFHHA
                     LNAAGVPHHYEMYGRPAPGQKPFGCDGGHNFSCWNYAFFDVLPRMLAVLDGP"
     misc_feature    complement(9911..9913)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            10383..11267
                     /locus_tag="BLU09_RS29065"
                     /old_locus_tag="SAMN04488504_113116"
     CDS             10383..11267
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941491.1"
                     /locus_tag="BLU09_RS29065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_113116"
                     /product="hypothetical protein"
                     /protein_id="WP_090493273.1"
                     /transl_table=11
                     /translation="MGTITRVTTTLLKQGARGVSRSGGHRAPTRVLPQVAHHSTKAPSP
                     SQIASETRARNNSLDLMSDIMDAMDRRDGGNNAATPRIKPDVPLSRRENFAFDAIQPGY
                     GTSKSAIDKHGKLQPAGDSVTTSASDHVDGEKSIKAKTPYTSFSGPPVGVDGTATGPLV
                     DNPLYGKDRIVTNPAKDPKSGVVLDQFDIQRDLRTSTRPGFDETAVLRSEPRPQPLWQP
                     TPEERAIMPQVGVDPEKPSFTFRERAMVNSARDKEYLVKGTIHEFDFFQGQPDGSLKPL
                     SKQDVIDEARKKS"
     misc_feature    complement(11686..11698)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 18.52"
                     /tool="antismash"
ORIGIN
        1 cgtacgtcga ccaccgcaat caagtcacgc gcgtttccag agcgagccat tgaacgacca
       61 ggaatccgag ctgaacgatg acgtcgccat ttccgcacgc gcccggggcg aatcgctcgt
      121 cgtgcatgca atccatggcg ttcatgttcg cacctggtcg tcgcctggcg tcgagagcgt
      181 ccacacctcg caccggacag gcataccggc ccccctcacg acagggcact acgggccttt
      241 cgaagcagcc atgcatgtgg gaacacggtt tcaggagcct tcagcgcccc caccgtccgg
      301 cagggcccag ccccctcgga agcgctccgg gcggagacgt cactgaaccg ccatcggtag
      361 ccagggcggg cgccccccgt cccccacccg cccggcaatc accccccctt tcggtccacc
      421 cgctccaact tgcgccgcgg agaaccttcc tcgggctcgg gccgtaacga ggccagctgg
      481 tcggccgccg cgtccgccgc catgtgccgt acggtcgcca ggacgtcttc gaatcgcccc
      541 gatgcatcca agcggtcggc caggtcccgt gcctttcccg ccgcacgtgt atacgcgcga
      601 cgctccagcg ccgaggccgg caagagggaa gccgacagga agcccagggc caggacccca
      661 agtcccgccc ccacgggatg ctgccgcatc gtctgcgctg tccacgtctg ggctcccacc
      721 gcgcgggagc gcgcatcgtc tcgcgaggga aacttctctc tgaggctccc agcaaccgcc
      781 cggcccttgt cgcggaccgt ccgcaacggc gccagtcccc cttgcgtctg acctggagcg
      841 ccgccgttcc gtccctcgcg cgcagtcgtc ttgtccgcgc tacgtctcat gactcagcct
      901 ccctttccac atcacctgca ccgctggcgc gaaacatccg ccccgcgagc aggcctgccc
      961 ccagcagtcc cgtgagcaac aatctcgggc gctgccgcac gaactgtccc gtgcggtcca
     1021 ggaatgcctc gacagattgc gcatccagcc gcgaggaggc gccgcgaaac atgcgctctc
     1081 ccgtctccag catgcgttgc gatgccgggc tcggtgcacg ccgggtggcg ctgctcaacc
     1141 cctctgccat gtcctccagc accgacacga gcaagccccg ctggaagtcc aggcgctcct
     1201 tcacccggcc ccgcgcgtat cccgtgacct gttcgagctg cttctgcgca gcttccatcc
     1261 tgggtgatgc gcgtcgcgct gggccgcgcg agcgcttcct ctcctttgtc ttcgtcgagg
     1321 ccatgatgtg cgtcctcctg acaccgaagg tagacacctc cgcgtcacgt ccgggcaacc
     1381 agaccgctca cggcgtggtc gttcgcacgc acgtcccatg ggtaccttgc ggacatggcc
     1441 gtcgccacgt tgattcatca ggaggcgaac atgaagccag agcaggaaaa gacgcttcac
     1501 gaagaggccg tggcaacggt ggaccgcctg gtgctcaagc acccggagct gaagaaagag
     1561 ctggagcagg tagagggcta tgccgtcttc ccctcgctgg gcagggcatc gctggtcctc
     1621 ggcggctcat acggccacgg cgaggtcttc gagaagggca agccgattgg cttcgccacg
     1681 ctgagccagt tgaccctcgg agtccaggtg ggcggacaga ccctgagcga gctcatcttc
     1741 ttccatgacc gtgatgccct tgaagcgttc aagggaggaa aggtggcctt cgccgccaac
     1801 gcctccgccg tgctcgtgaa ggcggcggcg tccggaacca tcaactatgc gaagtgcgtt
     1861 gctcacgcct actcgcgtgg cggcatgctg ctcgaggcct ccctgggcgg acagaagttc
     1921 acgttcattc cgcagtcggg cgcatcggag cgagggaacg gaggcctgat gcagcgcttc
     1981 cgcaggcagg atgcgtccga ggagaacggg gccgatgaca ctgggcagcg tccgcggctg
     2041 caagcatcgc agccccctcc tgccctgacc acagggctga cggctgccgc cctgctgctc
     2101 acccggatca tcaaggcgca ggccagtgct cgccacgaat gaagcgtccc tccgagacaa
     2161 actggcgagc caaggccacc gcgcgctgaa gctcgcgagg aaggagctga gcgtccaccg
     2221 cagactgcat ggtgacgcac gggcggcatt gcaccggctg gaggcccgct caccggagct
     2281 ggctcaggcg agggctcggg cctacgccta tgccgtcttc ccttcgctag gccagggaag
     2341 cgccgtgctc ggaggcacct gggggatggg cgaggtcttc cgtcaggagc ggctggtggg
     2401 atacgcggcg ctggtccaac tgaccctcgg agtccagttg ggcggacaga cagcttcgga
     2461 ggtcgtcctc ttcgaggacc aggaggcgtt ccaacgcttc aagcatggcg ggacgggact
     2521 ggcactcaac gcggccgcga ccctggtcaa agcaggcgca gcgagggcgc ggggcccggc
     2581 tggtagcacg acggtcgcgc tggccacgga tggaggaatg tgggcgggcg tcgcgctcgg
     2641 cgcccagcgc gccttcgtcg ttccggccgt cctcacccgc agtagcacat tgcgccgggt
     2701 tctctcatcc ataccttctt tgacacgagg aacgccggcc cccaacgagg gcgaaacaag
     2761 ggagagcgcg atggccaagg cgcagacgac caaatccgga agaaaaaggt tgggcaagct
     2821 caccgcggcc tggaagcgcc gcaaggccca cgacgaagag aagacaggcc ccaagaaggt
     2881 ggccgtgcat acacgcgaag ccatgcaacg ggtcagcacc cgagggcgcg agacactgga
     2941 gcatctgcga gaccgcgtcc ccagtggaga cagactccgt gagcgagcag gccaggcacg
     3001 cacctgggca gccgaccaac tgggtgaaca cgcggtggcc atcggcctga ccacgctggc
     3061 agcaggcgtc gcgggagcgg cgctgctccc cgtgtcgaac cgggagcgcc gcgcgttgag
     3121 ctccgtgtcc cacaaggtgc agggactgac ccactccctt ggagccgccc cccaggttgc
     3181 gcggctgacc cggtctgtga gcgacgcggt ggcacgcgtc cgccgcggtg acggcgctgc
     3241 cacggaggag gagaaacccc gcgcccctcg ccccaagaag gtcgcggcga cgcgtggcaa
     3301 gaaagccgcg ggaaggccag cagcggccgc caagacatcg cgccggcccc ggaagggcag
     3361 ccctccgccc caggccagta agtagggacg caggccggag cgcccaacgg aacctcgcct
     3421 gttcccgctg ggcggaaggt gaaatcggac ggggcgttcg tggagggatt ggccacgaat
     3481 gtccgcgtcc gcattcaccc ggccacacag tcaggaccct ggccccacgg cctctcgcaa
     3541 gacgcctccg cggcctgaat caggcgccgt gcaggccctc ggccatgcac gccccgctgg
     3601 cgcctgaaag gatttcaagg gcggcgcgtt ggcctgacaa gagcgctccc tccatccagc
     3661 cgggcatggg ggaggtttgg tctccggcga agtggaccct gccttcgcgc gttgtgagcg
     3721 cacgccccca ctgcgtgagc tgcctcggcc ggaaccacgc aaaggccccc cgagcccaag
     3781 ggtccaggtc ccatgcgtag gaggttccgc actgcacttc ggaggacagc tccggcgcga
     3841 actggctcag gccccgcaca gccatggcaa tgcgctcctc ctcggagaga aggcccgccg
     3901 cgcgggcgca cgggcccacg cgatacgctt cgaggatgcc tgacggcgga ggttgtgtcg
     3961 gtgcggcgtc gaggacatgc atgatgggga ggtccgtcag ccacccactc gaccaccccc
     4021 actctgtcca gaggcggcgc cggtattgaa tgaaggtgcg cacgacggac gtatgctcga
     4081 gctcgcgaat cgcccgtagc ttgtccgccg agaaagcagg gacgagctca acgccacgaa
     4141 ggactgagaa cggaatggcg cacacgagcc gctccgctgt gaacgttctg tgttctcccg
     4201 tgctgccgcg gcagtacacg cggacggtcc cctgttcctg ctcgatgcgc ttcgcagccc
     4261 atccgaactg aatccttcca ccgagcgctt ccgccagggc gacgggcagc cggtcacttc
     4321 cgcccctcaa ggtgtaggcg ctcgagccgc ctctgccgac cgtcagcaac tcccgtcgaa
     4381 gtaccccaag cgccgagctc gcgtcgatac catcacccac gaggtcaaac cgtcccaggc
     4441 tcaccgcgtc aatggccgct gaagaagcgc cccgttccgc gagggcctca cgaagggtca
     4501 ggccatcgag gtgggtcgcg cctggaccgg gccaggactc atggaagggg tcgccgacag
     4561 cctccacgag gagagacacg tacctccgat agaggccaga aaggccgagc tgctgctcat
     4621 ccgccctcag gacatgcggg aggcccgtgt catcaccgaa gggggccagg atgcgacgtc
     4681 cccgaagatg gaagggccgc aaggccgacg tgtgcggaac tggcagcggg tcgagctcca
     4741 ggccgagcct cctcgccaag ccgaggaccg tcgagtgccc gctgaggaca tacttcgcgc
     4801 ccgcctcggc gtacattccg tccacgaacg gctgccgcag ggtcaggacc cggccaccaa
     4861 ctcgagagcg cgcctcgagc acgacgacgt cccggcctgc gaggacaagc gcatgcgctg
     4921 ccgccagtcc tgcgaggccc gcgcccagaa tgatgacgcg ttcgctcatt cgagacgtcg
     4981 cctcgcgcct tcggtgggac tcatgccagc ggaagcaggt acggattcag cagccctcgc
     5041 ctgagctcga gcggcgatga gggcagttgg ggattgcgcg gggggaggcg gcgcggcagc
     5101 agcccactcc cgaggcgtaa caggctcgga cagaacgcca gaggctgcgt cccaaggacc
     5161 agcgcctggc aagtccggaa cggagaggcc gccgccagtg tgagattgat gaccagggtg
     5221 cgcagcccat gctgtcggaa cctcgtgacg agctcccccg ccgtgtcggg gactcgaacc
     5281 cgagggagcg ccaccggggg gccttccagg agcaggtcga cagcgctccg catcgcgggg
     5341 ttcaagtaga gcaacgtgat gtcgaggagg gagtcgatga gccagtagtt ctccaggacc
     5401 gggggccgcc ggagcgcctc gaagtcaatc gcaagctccc gacgaagggc caccgcctcc
     5461 gtcaacattc gcatttcacg gagcgctcgg tgcacggcca ctgccgggtc tcccgcggct
     5521 gcggcggcga ggagcgtccc tcctttgggg aacgccccct ctcctgtcgc ctcggcgatg
     5581 agtgcgatga cgtgaaggcc ggcgcgaccg ggcaggtacg agcagtggag agtccagcac
     5641 gaacccttga aatgacgcga caccccctca tcaatgtcga gcgtagccat gtcgaggcgc
     5701 cgcgcccctt gacctcggta ccaggcgagc tggagcgcat cgcgctctac cacctccagg
     5761 agcgcggacc gcaacgcggc atcatcgctc gtatgtgtcg cgacgccatt ggatgaggca
     5821 cacaggaagc gcgggcccac gagccgttcg gaagtcgtga aagctgcagg aacgagcaat
     5881 tgctcaccgg tctccgcatc cgtggccctg acccaatcca gtggcgtgtc ctgggtgact
     5941 ggcgcgtagg gaaaccccgg gcttgcgtac tgctcgggcg tgtagaggtc ccaatcaagc
     6001 atgggcagga acggttgctc gagcgcacgc gccggagtcg cgcagacgtc gggcacacga
     6061 aggagggacg aggcacgctc gatggcctct gctcgcgcag tcagcctccg ctcctccagg
     6121 gatgctccga cgcccacctg ggccaggaaa tgtcctgaca ccacgggacg acctcgtttc
     6181 cgaagacgca gggtggcggc tccccagctc agggtgacaa tgggaggagc cccccgcctg
     6241 ccacggaccg tggcttcgcg cgtcgcgatt cccaggcgcc ccagcgcggc accagccatc
     6301 cgcgagccct ccagcttgcc gatgtggttc gccgcgcccc ggttcgcgga gcacgcgcat
     6361 cccgccgcgg gccgagaacg gatgcgtctg gtgcctggcc gcccgtaggc gccctctgtc
     6421 caggcacgcg catcgcggga atgcggcttc atggctttcc ggagcgccgt cagatcggct
     6481 gtccccagcg caatcacggc gcggcgcggg agcgcattcg ccactgcggc ggcctcatcg
     6541 ggcgcatccg cgctctgaac ctcccagagc agaccacagg cgatacacac accgctgcca
     6601 gcgcttcgcc aacgtgtggc gctcagctgg atgagtgctc cgctgttctc ggagaggtcg
     6661 gccgtgcgaa gaggtcccag tcgcagcctg ccggtgattc ccaattcctt gcggagggct
     6721 tgctcgtcca tccagcgtgg cgcacctcgc cccagcgccc cttcctcttc gagccgtgct
     6781 ccgagctccc tggcgccggt agggtcgaca cccgccttca ccagcgcgcg gacgaccgcg
     6841 gcgctccctc cgcggcgaac gtctccgagg atggagacga gaagagcaaa ggggaggccc
     6901 cgtatctcca tcagcgccct gcgggtctga aggatggagc tccccctcgc catttcgagg
     6961 agcacgacac ctgattgaac gcccatgtcg tgcccgggac gccgctactt cgaaatgtgg
     7021 gaaatgctgg acacgttggt ttcaaaggcc ttgagcgcgg ataccttcag aacgcgcacc
     7081 gtgaccttga gagtctttgc acgcttcgta cgagcaggct tcttcatgtt cggcctccct
     7141 tggtgagcaa gaagtgtctc ttcaagccag actccgagtc aacgacatga cagggcactt
     7201 ccggccgtga tgggaattgc cgtggcccac acaggagatg ggcctacctg tcagagcgca
     7261 acaccaggag ttctgacaat ccatttccgt gtcgagctga cctcaagtca gtccgcatcc
     7321 gcgcagccct caaccaggcg ctacgtcgct tcggattccg catgttcggg caccaccgcc
     7381 ttgagtgact ggcttccggc gctgcttcca cgaaacaaac cctcgatgaa cacgggacga
     7441 tatgtccgcg ccgggccctt cgttctgaga gggccagccc cttcccattc agggggcgtg
     7501 tcacatcccg tctcgagtgc acatgaaggg ccccgaatgt ccaagctcgg tctttcgttc
     7561 atcgtcttgt tcgcatgcac gtccgcattc gcatccccac ctccgcggat tgaaactgaa
     7621 gcggaatcgc tcgcgtcaac accgacaccg ttcgcggtgc tacgtgagtt cgtgaccgaa
     7681 gacatctatc actaccgatt cgacattcgt gtcggcaccg caccaaacgc catcgtgcgg
     7741 gtccatcgcg tcgtccgaga gcgtgcgccg tggcggccac ggtcgcctgc ccacgcgatc
     7801 atgttgcttc acggagactt cgccaacttc gtcacgaact tcttgccgac actcggcatt
     7861 cccgcgtcat cggcgccggg gctcgctccg tacctggcgg cccgagacat cgacgtctgg
     7921 ggcgttgacc gtcgctggac gctgccctcc gccaacggag acatctccga cctgggcgag
     7981 atgggcgttg cccaggagct cggagacatc accgtcgcgc tcaccttcgc acgggcggca
     8041 cggctcagcg gcgaccgtga caagggccga atcgtgctcg gcggattctc gcatggcggc
     8101 gagctggcct acgcgtatgc cgccgccaac ggccgccacg tgtcggcggt cgccgtgctc
     8161 gacgcctact acgacatcgc gcccgagcac gcggaactgc gcgagctggc gtgcgccaat
     8221 gccgccgcgg gacgtgaggc gcttgcgcaa ggcgtgaccg actccgacaa cagcttcttc
     8281 atcacgttgg gcgagctggc ccggagcgcg ccccaggcgc agtctcccgt gttttcgagc
     8341 tacaccaacc gcggtgcact gctcgcgatg aatggcctgg cctaccttct cgcgccgtac
     8401 acgccgctgt accacttgag cgcgcccatc ctcgacgcca acggcgacgt cacggcattg
     8461 cgcgagtcgc ccgaggacag cgtgagcgcg tggttcgcca gcgcgccgcc gcaccagtcg
     8521 ctgcgtgaga ccgtggaact cgacgaaatc tggtgcggga cgacacccag gcccgagctc
     8581 ttgaacatcc gcgtcccgtt gttctacctc ggtgccgctg gcgcattcgg tgactacggg
     8641 ctgtactcga cgacccgggt gtcatcgacg gatgtcacgg cgctcgtcgt ccgccgcttc
     8701 ggtccggagc gagtggccga ggacttcggc catggtgacc tcttgtacgc ggcggatgca
     8761 ccgacgctcg cgtggcaacc gctcgcgacg tggttgctga gccattgagg aggagcatct
     8821 ccgtaccaag aggccgcaag aagaaggcgc tcggctcgcg cgcgtggcag tgggagccct
     8881 tcgcatcgga cgaagggctc catcacctga agtgccgttg ggcttacggc ccgtccagca
     8941 cggccaacat gcgcggcagc acgtcgaaga acgcgtagtt ccagcaactg aagttgtgcc
     9001 cgccgtcaca accgaagggc ttctggcccg gcgcggggcg gccatacatc tcgtagtggt
     9061 gcggcacgcc cgcggcattc agcgcatggt ggaaccggtc cgtggacgcc cccgggacgc
     9121 gttcgagcac atccccatcc cagatgcccg accccgcata caaggcgacg gccacgcccc
     9181 gcagagacgc cgcacgcgga atggggttgt tcatgttcca ggcctggtcg aagggccaga
     9241 agggagagcc gaacgcccca tccgggttct gcagccaccg cgtcccctcc tccgtgatgg
     9301 tggcgcggat ggcggcgtca cccaggtcca gggcccccga gaaactggcc gcataggcaa
     9361 acaggtcggg gcgccgcgcg gcgtagctga tggccccgaa accgcccatg gacagccccg
     9421 cgatagcacg gccccgcttg tacgccagcg tgcgaaggtt ctggtccacc cacggaatga
     9481 gctggttgag gtggaactcc tcccacgcct gattcacccc acgagggaag acccagttcg
     9541 tgtaccaacc gaccttgccg ccatccggca tgatggtgat gacgggcatg ttctcggtaa
     9601 tcgcatacgc cgcaccgaac tccacccact gagccgagtt cgcgcctcct cccccgtgca
     9661 acaggtagac cgcggggtat cgcacgcccg ggttgttgta ataccccgtc ggcaggagca
     9721 cccgaacgtg gtggcgaggg tcatagaccg cgtgaggtgc gatcagcggc gtggagattt
     9781 ccacgtcgat gagccgatcc gtgatgtgcc gcaccgcatg aaccgtgatg ccatgccccg
     9841 aatagaagtt tggcgcgagc gaggcatcgg atgcctccgc gtcacctggt actccgagga
     9901 acagggcgaa taacaagact cccacgagtc tgcgtgcggc acggacggga gactcgaatc
     9961 gttcaagagc ggtgctggaa acaaagacgt cctggggcag caatctgcgc atcctctgac
    10021 ctcggagagt aggtgggccg acaacggctc ttcattcgcc aatgacaggt gcgacgctag
    10081 ggggcgtatc tggccatggc aacgcacacg ttggagcgac cgtgtcattc cctcgcaagg
    10141 gccgctgagg ctgtgtcttt tccgcgactg tggacgagag ctgtccatta attgcattcc
    10201 gtggcgactc gcttcacccc ggaaggcccc caccctcgaa tgatggagac ctcgaatggg
    10261 acacagctgc gccctcaccc caatcgccct cccccacgca actccgtgag cagatggagg
    10321 ataattcgca caaaggcatg aaggaacgca cctcatgccc cagaccaggg ggggtctaca
    10381 tcatgggtac catcacgagg gtaacgacga cgttgttgaa gcaaggcgca cgaggagtgt
    10441 cccgctccgg cggccaccgg gcacccaccc gcgtgctgcc acaagtcgcc catcactcca
    10501 ccaaggcacc aagcccgtct caaattgcga gcgagacgcg ggcgcggaac aacagcctgg
    10561 acctgatgag cgacatcatg gatgcaatgg atcgccgtga tggcggcaac aacgcggcca
    10621 cgcctcgtat caagccggat gttccgctgt cgcgccgtga gaacttcgcg ttcgacgcca
    10681 tccaaccagg gtacggaacg tcgaagtccg ccatcgacaa gcacggcaag ctccagcccg
    10741 cgggcgacag cgtcaccacc agcgcctccg accacgtcga tggcgaaaag tccatcaagg
    10801 cgaagacgcc gtacacgtcc ttcagcggtc cgccggtggg cgtggacggc acggccacgg
    10861 ggcccctggt cgacaacccg ctctacggca aggaccgcat cgtcaccaac ccggccaagg
    10921 accccaagag cggtgtggtg ctcgaccagt tcgacatcca gcgcgacctc cggacgagca
    10981 ccaggcccgg cttcgacgag accgcggtgc tgcgctccga gccacgtccg cagccgctct
    11041 ggcagcccac acccgaagag cgcgccatca tgccccaagt cggtgtggac cccgagaagc
    11101 cctcgttcac gtttcgcgaa cgcgcgatgg tgaactcggc gcgggacaag gagtacctgg
    11161 tcaagggcac catccacgag ttcgacttct tccagggcca accagacggc tcgctcaagc
    11221 ccctctccaa gcaagacgtg attgacgaag ccaggaagaa gtcctgagcc accgcgcatc
    11281 gtcccatcac cgtgccagac gaaccccgac cctcgcggcg ccgtgttgca ccgcgagggc
    11341 ggtgagcccg ccagctcatg atgtcattca gcgagcggca gcgtcggtcc aggctacccc
    11401 atgacgcaga cgcccgacga gcaggtcttc gccgccggac agacaacgcc gcaggcgccg
    11461 cagttgtgca cgtccgacga cacatccacg cactgcgcgc cacactgcac ccggggagcc
    11521 ggacaggagc cgcccacgca ctcatagcca cccggcgtgt tgacgcacac ctggctggga
    11581 ttgcatgcgg cagtcccgtt cgagcactcg tcgatgtcac tgcacgtcca accatttccc
    11641 cagtagcccg gcctgcaggt gcaggtgtag ctccccggcg tgttcgtgca aatcgcattc
    11701 accgagcact gcgcagtgcc attcgtgcat tcattgacgt ccgtacagac actgggctgc
    11761 atgccattgc aggtgtaccc cggctccacc cggcaggtgc tgctgcagcc atccgacgaa
    11821 acccggttgc cgtcgtcaca ctgctctcca ttgctgatac ggccatcgcc acacacggcg
    11881 ctcacaccgc cgcccgtatc ctccaccgcg tccaagccga acaacgaggc aaccccactg
    11941 cgcagcctca cgtagcgata cggcgtggag ttgctgaatg gcacca
//
