LOCUS       NZ_FNAJ01000007        10864 bp    DNA     linear   CON 22-APR-2024
DEFINITION  Myxococcus virescens strain DSM 2260, whole genome shotgun sequence.
ACCESSION   NZ_FNAJ01000007
VERSION     NZ_FNAJ01000007.1
KEYWORDS    WGS; RefSeq.
SOURCE      Myxococcus virescens
  ORGANISM  Myxococcus virescens
            Bacteria; Myxococcota; Myxococcia; Myxococcales; Cystobacterineae;
            Myxococcaceae; Myxococcus.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900101905.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 02:08:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,594
            CDSs (total)                      :: 7,524
            Genes (coding)                    :: 7,456
            CDSs (with protein)               :: 7,456
            Genes (RNA)                       :: 70
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 68
            CDSs (without protein)            :: 68
            Pseudo Genes (ambiguous residues) :: 0 of 68
            Pseudo Genes (frameshifted)       :: 15 of 68
            Pseudo Genes (incomplete)         :: 65 of 68
            Pseudo Genes (internal stop)      :: 7 of 68
            Pseudo Genes (multiple problems)  :: 17 of 68
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 04:03:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 273686
            Orig. end                         :: 284550
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNAJ01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10864
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10864
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10864
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5864](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5864)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    20..29
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     misc_feature    64..78
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.35"
                     /tool="antismash"
     gene            168..998
                     /gene="rsmI"
                     /locus_tag="BLU09_RS21930"
                     /old_locus_tag="SAMN04488504_107245"
     CDS             168..998
                     /EC_number="2.1.1.198"
                     /GO_function="GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="rsmI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TP_methylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553581.1"
                     /locus_tag="BLU09_RS21930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_107245"
                     /product="16S rRNA (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="WP_011553581.1"
                     /sec_met_domain="TP_methylase (E-value: 3.6e-31, bitscore:
                     103.1, seeds: 87, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGTLYLVATPIGNLGDVTSRALETLRAVRFIACEDTRHSRVLLD
                     HFGIGGKDLVSLPAFAEGQRAGRILDRIGEGEDCALVTDAGSPAISDPGEKLVAEALER
                     GLTVVPVPGPTALVAALSASGLPTGRFHFLGFLPRKGAERRAMLEEVAQLSATLVLYES
                     PRRLSETLPDLLDAWGDRRACVARELTKLHEEFARGTLSELAARYAAEEPRGEVVVLVE
                     GRTGETRWSEEELRRALEEGLSRGEKLKALSTELARRAGWAGQDVYRLGLSLKR"
     misc_feature    543..545
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(1040..2335)
                     /locus_tag="BLU09_RS21935"
                     /old_locus_tag="SAMN04488504_107246"
     CDS             complement(1040..2335)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553582.1"
                     /locus_tag="BLU09_RS21935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_107246"
                     /product="hypothetical protein"
                     /protein_id="WP_090491466.1"
                     /transl_table=11
                     /translation="MHRLRVVLAALTLGAPFAATAADITRIASSLEEDDPFDLFIDVGF
                     ERTQNRAKIIREQLAPAGQPAGRRDASELWYKGVDARLNLELSVGLYRDLEFSFALPVV
                     FSQNERWDFTSNSSPETSTITNNCLNANGSVIPGCLEGSAPGSALFDVPQESFRGGLGN
                     MRFGLAYAFFNQEKDETKPTWIVGLEYEAPTAKLRDAAADTTNSDSRGNIGDRVHKYQL
                     YTAFSRRIGAAEPYFKAHYTIPVRGPGAYSNCVQKDSNPDNLGAPDNCFTGPWNRKETG
                     INAPHTAGMLFGVELVPFNRPAKSQRFALDVRGIGNYVGSGRYYNELSAALRKLLTSED
                     YFQVGGMLGVTANASQAFQIRASGMFLYNTNHALTAEAPGRDANNDGGVDTQPGSPDLN
                     PNFDWRYDLVSRRFLAVESTTFRFDLSATFSF"
     gene            complement(2432..3598)
                     /locus_tag="BLU09_RS21940"
                     /old_locus_tag="SAMN04488504_107247"
     CDS             complement(2432..3598)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553583.1"
                     /locus_tag="BLU09_RS21940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_107247"
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="WP_090491467.1"
                     /transl_table=11
                     /translation="MACATPHQTGRKTVRTNRPRGSGIPLRARRFRARPPIPTQGACMR
                     PNLPTLGAPPKRSPFGTVVAVSLILGGAAGGVWWWKQRMVQATEEAAAQMPNAVPDAGA
                     VAEAPTPAPVATDPVKAAGLERVSVSINGPLETALVNAAGSDVGPALAQVVTRTLVWWV
                     SVPGEILRGDTLEVLYQRRTNEEPLVHAVRFTSAKLGKTLSAYRYQANGEPNARYYLSG
                     GDELELRLERSPIDDYEQVTSLLRDGRKHKGVDFRTPVGTPIKAPFNGVVKRKNWNFSS
                     NGNCIELVESGGKGRRALFLHLDEVDKSVKPGTRFSVGQVIAKSGNTGRSFAPHLHYQL
                     MTQDDRVLDPYDQHKTYRRSLAAQHRSGFEAEVRRMDGLLSASVVAGK"
     gene            complement(3546..4970)
                     /locus_tag="BLU09_RS21945"
                     /old_locus_tag="SAMN04488504_107248"
     CDS             complement(3546..4970)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1112: sigma-54
                     dependent trancsriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940152.1"
                     /locus_tag="BLU09_RS21945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_107248"
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="WP_090491468.1"
                     /transl_table=11
                     /translation="MAKVLVIDDEANLRKVLAAMLRRDGFDVTVAENGEQGLAEFHKNG
                     ADIVVTDLVMPKVGGMEVLGTIRTANPDVPVIIITAHGTVDSAVDAIKAGAFDYITKPF
                     DQAELSSVVAKAAKTNESARRSVRPDVKARAAIIGDSSTIQEVYKIIDKVADTPSTVLI
                     TGESGTGKELIATALHGASSRRDKPFIKINCAAIPHTLLESELFGYERGAFTGAVTSKP
                     GRFELADEGTLFLDEIGEIPVEMQVKLLRAIQEGEFERVGGIKTTRVDVRLVAATNRDL
                     QAEIEAGRFRKDLYYRLAVVPISLPALRERRSDIPMLARHFVEKYNRRLNKKIEGIADD
                     AMALLQGYAWPGNIRELENLIERVLLFADGPLITARDLPEPVRQGTSVQASTNLASAVA
                     SIDVPTGEVGLKDIVRMKAAELERDLIVKKLEETGGNVTRAARLLQISRKSLQTKMKEF
                     GLRDTTPDGQEDGPDE"
     gene            complement(5001..5864)
                     /gene="encA"
                     /locus_tag="BLU09_RS21950"
                     /old_locus_tag="SAMN04488504_107249"
     CDS             complement(5001..5864)
                     /codon_start=1
                     /gene="encA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114001.1"
                     /locus_tag="BLU09_RS21950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_107249"
                     /product="encapsulin nanocompartment shell protein EncA"
                     /protein_id="WP_090491469.1"
                     /sec_met_domain="Linocin_M18 (E-value: 3.9e-84, bitscore:
                     276.0, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDFLGHAENPLREEEWARLNETVIQVARRSLVGRRILDIYGPLG
                     AGVQTVPYDEFQGVSPGAVDIVGEQETAMVFTDARKFKTIPIIYKDFLLHWRDIEAART
                     HNMPLDVSAAAGAAALCAQQEDELIFYGDVRLGYEGLMTANGRLTVPLGDWTSPGGGFQ
                     AIVEATRKLNEQGHFGPYAVVLSPRLYSQLHRIYEKTGVLEIETIRQLASDGVYQSNRL
                     RGDSGVVVSTGRENMDLAVSMDMVAAYLGASRMNHPFRVLEALLLRIKHPDAICTLEGA
                     GATERR"
     gene            complement(5913..6389)
                     /gene="encB"
                     /locus_tag="BLU09_RS21955"
                     /old_locus_tag="SAMN04488504_107250"
     CDS             complement(5913..6389)
                     /codon_start=1
                     /gene="encB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_026114000.1"
                     /locus_tag="BLU09_RS21955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_107250"
                     /product="encapsulin nanocompartment cargo protein EncB"
                     /protein_id="WP_167371126.1"
                     /transl_table=11
                     /translation="MAGSPDSDLDDVARIRLVLARELETINEYEAYARASSNPEVRAFF
                     QHLAAEEKEHVSEAVHMLRMLDSGQNDHFAKPFVPGHFQAAEAPAPATVHVPAPDGPAF
                     SVNGRNGRLPSEPPTSLPPQRLLYGLPAPPPAVESHPLTVGSLRRGGGGSGSGR"
     gene            6495..8192
                     /gene="murJ"
                     /locus_tag="BLU09_RS21960"
                     /old_locus_tag="SAMN04488504_107251"
     CDS             6495..8192
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /GO_process="GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="murJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940155.1"
                     /locus_tag="BLU09_RS21960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_107251"
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="WP_090491471.1"
                     /transl_table=11
                     /translation="MTTSSPESPPPPIAPVAPPRPERSGGGAALVAAGILASRLMGLVR
                     ERVFAHYLGNTEVAAVFKAALRIPNFLQNLFGEGVLSGSFIPVYAQLLGRKDTETADRV
                     AGAVFGILSLVTAVVVALGMVFTPLLVDAIAPGFQGQERELAIHLVRILFPGTGMLVLS
                     AWCLGILNSHRRFLLSYLAPVVWNLVIIAALVAAGGRYEEEALVSVLAYAVVLGSFLQF
                     AVQVPSVLKLMGRFRPTLSLAAEPVRQVLKNFGPVVLGRGVVQFSAWVDTAFASLISNR
                     ALSSLLYAQTIYLIPVSLFGMAVSAAELPEMSRATGEGADVAARLRQRIDGGARRICFW
                     VVPSAAAFLFLGDMVAAALLQTGRFDAADSRYLWYLLMGAALGLVASTVGRLYASAFYA
                     LKDPKTPLRFAVVRVLVGTLGAWVLGLRLPGWLGVPQHLGALGLTLASGLVAWLEAGLL
                     RRKLVKQLGPVGVPSGLLPRLWGAAVVAGLVAVGIKVGLTSLLGPMPGVTAEWGGGFLM
                     PPRLHPVLGFAAVALPFGAVYFGMSAALGIPEAGTVLRKVGRKLRLVR"
     gene            8465..10405
                     /locus_tag="BLU09_RS21965"
                     /old_locus_tag="SAMN04488504_107252"
     CDS             8465..10405
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940156.1"
                     /locus_tag="BLU09_RS21965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN04488504_107252"
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="WP_090491538.1"
                     /transl_table=11
                     /translation="MGPKKPKATFGDVMLGIPSGGARSEGGRGEQRGGGRGGERSGGGR
                     GERDAQPRPAGGAPRDERGPRGGSERRGGGDRRPSGPMVVVKRASGAIETRGPVGDAPA
                     EATATAEETTAAAESSAAPPAPTPRPVTPTPAPTSALYEEVPESQSFAEMFEAQAKEGG
                     TPSRKGLRVGEKVRGTIFQLGADTAFVSVEGAAKSEAMIELRELKDDEGILRFGVGDTI
                     DAHVVEVGAKGILLSRALAKGSANLALLAEARASGMPVEGLVLSVNKGGVEVAIGDIRA
                     FCPISQLDLRYVEKPDQFIGEKLQFRVSEVRERNVVLSRRSLLEDEQRQLAAETRKNLA
                     QGKIVKGKVTGVRDFGVFVDLGGVEGMIPVSELSYTRVGHPSDVVKVGDDVEVEILRME
                     AGQPNSPDKSKQKERITLSMRSRQEDPFQKALSEIKEGDRLQGKVVRLQQFGAFVELRP
                     GVDGLVHISALSDRRIAHPRDVVKEGETIWVSVEKIDTQEKRIGLRRISEEEAQRPPEE
                     RTAPAAEAAAPAQPAAPRPKVGQVVVGKVDRIEPYGVFLAFPGGKGLLPASETGTERGT
                     DLRKHYSLGQEVKVAILDIDASGKIRLSVTAAIRAEERAEVEAWQKTQQPQGAGKKGFG
                     TFADLLSKARK"
     tRNA            10507..10580
                     /anticodon=(pos:284227..284229,aa:Met,seq:cat)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="BLU09_RS21970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SAMN04488504_107253"
                     /product="tRNA-Met"
     gene            10507..10580
                     /locus_tag="BLU09_RS21970"
                     /old_locus_tag="SAMN04488504_107253"
     misc_feature    10586..10595
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.11"
                     /tool="antismash"
     tRNA            10648..10721
                     /anticodon=(pos:284368..284370,aa:Met,seq:cat)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="BLU09_RS21975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SAMN04488504_107254"
                     /product="tRNA-Met"
     gene            10648..10721
                     /locus_tag="BLU09_RS21975"
                     /old_locus_tag="SAMN04488504_107254"
ORIGIN
        1 ccaagcagcg ccgggtggtg aaggcggcgc ttcggtacct gttcgcgcat gacttgcgtg
       61 gacggatgtt ccgtttcgac gtgatttcgg tggtgggacg cggggagctc gccaccgtgg
      121 agcacatccc tggcgccttc gacgcgggga tgtgagagga cgacgccatg gctggaacgc
      181 tctatctggt ggcgacgccc atcgggaacc tgggggatgt cacgtcgcgc gcgttggaga
      241 ccctgcgcgc ggtgcgcttc atcgcgtgtg aggacacgcg gcactcgcgg gtgctgctcg
      301 accacttcgg cattggcggg aaggacctgg tgagcctgcc cgccttcgcg gaagggcagc
      361 gagccggacg catcctggac cggattgggg aaggagagga ctgtgcgctg gtgactgacg
      421 cgggcagccc cgccatcagt gatccgggcg agaagctggt ggccgaggcg ctggagcgcg
      481 gattgacggt ggtgccggta ccaggcccca cggccctggt cgcggcgctg agcgcctctg
      541 gattacccac ggggcgcttc cacttcctgg gcttcctccc gcgcaagggc gccgagcgcc
      601 gggcgatgct ggaggaggtg gcgcagctgt ccgccaccct ggtgctttat gagtccccac
      661 gccgcctgtc ggagacgttg ccggacctgc tcgacgcctg gggcgaccgg cgcgcgtgcg
      721 tggcgcggga gctgacgaag ctgcacgagg agttcgcccg gggcaccttg tccgaactgg
      781 ccgcgcgcta cgcggcggaa gagccccgcg gcgaggtggt ggtgttggtg gagggccgca
      841 ccggcgagac acgctggtcc gaggaggaat tgcgtcgggc cctggaggag gggctgtcgc
      901 gcggcgagaa gctcaaggcg ctgagcaccg agctagcccg ccgcgcgggc tgggccggtc
      961 aggacgtcta tcgactgggg ctctcgctga agcgctgagt ccccaggtgg gcgccgtcat
     1021 cggcgcccac cgggccacgc tagaagctga acgtggcgct caggtcgaag cggaacgtgg
     1081 tgctctccac cgcgaggaag cggcgagaca ccaggtcata gcgccagtcg aagttggggt
     1141 tgaggtcggg cgagccgggc tgcgtgtcca cgccgccgtc gttgttcgcg tcacgccccg
     1201 gagcctcggc ggtgagggcg tggttggtgt tgtagaggaa catgccggac gcgcgaatct
     1261 ggaaggcctg cgacgcgttc gccgtgacgc ccagcatgcc acccacctgg aagtagtcct
     1321 cggaggtgag cagcttgcgc agggcggcgc tcagctcgtt gtagtagcgg ccgctgccca
     1381 cgtagttgcc gataccccgg acatcgaggg cgaagcgctg cgacttcgcc ggccggttga
     1441 agggcaccag ttccacgccg aagagcatgc ccgcggtgtg gggggcgttg atgcccgttt
     1501 ccttgcggtt ccacgggccc gtgaagcagt tgtccggggc gcccaggttg tccgggttgg
     1561 aatccttctg gacgcaattg gagtaggcgc ccgggccgcg cacggggatg gtgtagtgcg
     1621 ccttgaagta cggctcggcc gcgccgatgc ggcgcgagaa ggccgtgtag agctggtact
     1681 tgtggacgcg gtcgccaatg ttgccgcggc tgtccgagtt ggtggtgtcc gcggccgcgt
     1741 cacgcagctt cgcggtgggg gcctcgtact ccaggccgac aatccaggtg ggcttcgtct
     1801 cgtccttctc ctggttgaag aaggcgtagg ccagaccgaa gcgcatgttg cccaggccgc
     1861 cgcggaagct ctcctgcggt acgtcgaaca gggcgctgcc cggtgccgag ccctccaggc
     1921 agcccgggat gacggagccg ttggcgttca ggcagttgtt ggtgatggtg gacgtctcgg
     1981 gagacgagtt ggacgtgaag tcccaacgct cgttctgcga gaagacgacg ggcagggcga
     2041 aggagaactc caggtccctg tagaggccga cggacagctc caggttgagc cgggcgtcca
     2101 cgcccttgta ccagagctcc gatgcatccc ggcggcccgc cggttgcccg gcgggggcca
     2161 actgctcgcg gatgatcttc gcgcggttct gtgtgcgctc gaagccgacg tcgatgaaga
     2221 ggtcgaacgg gtcgtcctcc tcgagcgacg acgcaatccg ggtgatgtcc gccgcggtgg
     2281 cggcgaacgg cgcgcccagc gtcagcgcgg caagtacgac gcgaagcctg tgcatccgac
     2341 ctccgacggc aaatctccct ggcctaccgg caagcccggg gggcttccag ctagccgtgc
     2401 cgtcgaagga gtgtcaagaa acgggtggct tttacttgcc ggccaccacg gacgcgctca
     2461 gcaacccgtc catccggcgc acctccgcct cgaaacccga ccggtgctgc gcggccagtg
     2521 agcggcggta ggtcttgtgc tggtcgtagg ggtccagcac ccggtcgtcc tgcgtcatga
     2581 gctggtaatg gaggtggggg gcgaagctgc ggcccgtgtt gccgctcttg gcgatgacct
     2641 gccccacgga gaaccgggtg cccggcttga cgctcttgtc cacctcgtcc aggtggagga
     2701 agagcgcgcg gcgcccctta ccgcccgact ccaccagctc gatgcagttg ccgttgctgc
     2761 tgaagttcca gttcttccgc ttcaccaccc cgttgaaggg ggccttgatg ggcgtgccga
     2821 ccggggtgcg gaagtccacg cccttgtgct tgcggccgtc gcgaagcagg gacgtcacct
     2881 gctcgtagtc gtcgatgggg gagcgctcca gcctcagctc caactcgtcg ccgccggaca
     2941 ggtagtaccg ggcattgggc tcgccgttgg cctggtagcg gtacgcgctc agcgtcttgc
     3001 ccagcttggc gctggtgaag cgcaccgcgt gcaccagggg ctcctcgttg gttcggcgct
     3061 ggtagagcac ctccagcgtg tcaccgcgga gaatctcccc gggcacggag acccaccaca
     3121 ccaaggtgcg cgtcaccacc tgcgccagcg ccggacccac gtccgagcca gcggcgttga
     3181 cgagcgcggt ctccaggggg ccgttgatgc tgaccgatac gcgctccagg cccgcggcct
     3241 tcaccgggtc cgtggccacg ggcgctggcg tgggcgcctc ggcgacggcg cccgcgtcgg
     3301 ggactgcgtt gggcatctga gccgcggcct cttccgtcgc ctgaaccatc cgctgcttcc
     3361 accaccacac gccgccggcg gcaccgccga ggatgagtga cacggcgacc accgtgccga
     3421 agggactgcg cttcggaggg gcaccgagtg tgggaaggtt cggccgcata caagctccct
     3481 gagttggaat gggtgggcgg gcccggaacc gacgcgcgcg aagcggaatt ccgcttcctc
     3541 gcggtctatt cgtccggacc gtcttcctgc ccgtctggtg tggtgtcgcg caagccaaac
     3601 tccttcatct tcgtctggag tgacttgcgg ctgatttgaa gcaaccgggc ggcccgggtg
     3661 acgttgccac ccgtctcctc gagcttcttc acgatgaggt cccgctcgag ctcggcggcc
     3721 ttcatccgga cgatgtcctt caggccgact tcccccgtgg ggacgtcgat ggaggccacc
     3781 gcgctggcca ggttggtgct cgcctggacg ctcgtgccct ggcggaccgg ctccggcagg
     3841 tcgcgggcgg tgatgagagg cccgtccgcg aacaggagca cgcgctcgat gaggttctcc
     3901 agctcgcgga tgttgcccgg ccacgcgtag ccctggagca gggccatggc gtcgtcggcg
     3961 atgccttcaa tcttcttgtt gaggcgccgg ttgtacttct ccacgaagtg ccgggcgagc
     4021 atggggatgt cgctgcggcg ctcgcgcagg gcgggcaggg agatggggac caccgccagg
     4081 cggtagtaga ggtccttgcg gaagcggccg gcttcaatct ccgcctggag gtcgcggttg
     4141 gtggcggcca cgagccgcac gtccacgcgc gtcgtcttga tgccgcccac gcgctcgaac
     4201 tcgccctcct ggatcgcacg cagcagcttc acctgcatct ccactggaat ctcgccgatc
     4261 tcgtccagga agagggtgcc ctcgtcggcc agctcgaagc ggccgggctt ggacgtgacg
     4321 gcgccggtga aggccccgcg ctcgtagccg aagagctcgc tctccagcag cgtgtgggga
     4381 atggcggcgc agttgatctt gatgaacggc ttgtcgcggc ggctggaggc gccgtgcagc
     4441 gcggtggcga tcagctcctt gccggtgccg ctttccccgg tgatgagcac cgtggacggc
     4501 gtgtccgcca ccttgtcgat gatcttgtag acctcttgaa tcgtcgacga gtcgccgatg
     4561 atggccgcgc gcgccttcac gtccgggcgc acggagcggc gggcgctctc gttcgtcttc
     4621 gcggccttgg cgacgacgga ggacagctcc gcctgatcga agggcttggt gatgtagtcg
     4681 aacgcgcccg ccttgatggc gtccacggcg gagtccaccg tgccatgcgc ggtgatgatg
     4741 atgacgggga cgtccgggtt ggcggtgcgg atggtgccga gcacctccat gccgcccacc
     4801 ttgggcatca ccaggtccgt gaccacgatg tccgcgccgt tcttgtggaa ctcggcgagc
     4861 ccttgctcgc cgttctccgc cacggtgacg tcaaagccgt cccggcgcag catcgcggcg
     4921 agcaccttgc ggaggttcgc ctcgtcgtcg atgaccagga ccttggccat ggtggaagtc
     4981 gtgaccgcgg gcgaccggaa ctagcgacgt tccgtggcgc cggcgccctc cagggtgcag
     5041 atggcgtccg ggtgcttgat gcgcagcagc agcgcctcca gaacgcggaa cgggtggttc
     5101 atccgcgacg cgcccaggta cgccgccacc atgtccatgg acaccgcgag gtccatgttc
     5161 tcccggccgg tggacaccac cacgccggag tcccctcgca ggcggttgga ctggtagacg
     5221 ccgtccgacg cgagttggcg gatggtctca atctccagga cgcccgtctt ctcgtagatg
     5281 cggtgcagct gcgagtacag ccgcggcgac aggacgacgg cgtaggggcc gaagtggccc
     5341 tgctcgttga gcttgcgcgt ggcctcgacg atggcctgga aaccgccacc cggcgacgtc
     5401 cagtcaccca gcggcaccgt gagccggccg ttggccgtca tcaggccttc gtagcccagg
     5461 cgcacgtcac cgtagaagat gagttcgtct tcctgctgcg cgcacagcgc ggcggcaccg
     5521 gcggcggccg acacgtccag cggcatgttg tgcgtgcgcg cggcctcgat gtcgcgccag
     5581 tgcagcagga agtccttgta gatgatgggg atggtcttga acttgcgagc atcggtgaag
     5641 accatcgcgg tctcctgctc gccgacgatg tccacggcgc cgggtgagac gccctggaac
     5701 tcgtcgtagg gaaccgtctg caccccggcg cccagcggcc cgtagatgtc caggatgcgc
     5761 cgacccacca gcgaacgccg ggcgacctgg atgacagtct cgttcagacg cgcccactcc
     5821 tcttcgcgga gcgggttctc ggcatgtcca aggaagtcag gcatgaaatg tggctccaga
     5881 ggcatcgact agaagagaag gggaagaaag cttcagcgac cgctgccgga accaccgcca
     5941 ccccggcgca gtgaacccac ggtgagcggg tgcgactcca cggcgggcgg cggcgcaggc
     6001 aggccataca gcagccgctg gggcggcagg gacgttggcg gctcggaggg gagccggccg
     6061 ttgcggccgt tgacggagaa agccgggcca tccggtgcgg gcacatggac ggtggcgggg
     6121 gcgggggcct ctgcggcctg gaaatgaccg gggacgaagg gcttggcgaa gtggtcgttc
     6181 tgaccactgt ccaacatgcg aagcatgtgg acggcctcgg agacgtgctc cttctcctcg
     6241 gcggccaggt gctggaagaa cgcgcggacc tcggggttcg aggaggcacg agcgtaggct
     6301 tcgtactcgt tgatcgtctc cagctcacgt gccagcacga ggcggatgcg cgccacgtcg
     6361 tccaagtcac tgtcgggaga gccggccatt ggaacggacc cttgcaatcc agggggttcg
     6421 gcgtcaagcg aaacaggggg acggggtttc gggcttcatg acaccgaacg gcccgcgtag
     6481 agtccgccgc tctcgtgacc acgtcttcgc ccgagtctcc acccccgccg attgcgccgg
     6541 ttgcgcctcc tcgtcccgag cgctcaggag ggggggccgc gctggtggcg gccggcatcc
     6601 ttgcttcccg gctgatgggc ctggtgcgcg agcgcgtctt cgcccactac ctgggtaata
     6661 cggaggtcgc ggccgtcttc aaggcggcgc tgcgcattcc caacttcctg cagaatctgt
     6721 ttggcgaggg cgtcctctcc ggctccttca tccccgtcta cgcgcagctc ttgggccgca
     6781 aggacacgga gacggcggac cgggtcgcgg gcgccgtctt cggcatcctg tccctggtca
     6841 ctgcggtggt ggtggcgctg ggcatggtgt tcaccccgct gctggtggat gccatcgcgc
     6901 ccggcttcca ggggcaggag cgggaactgg ccatccacct ggtgcgcatc ctcttccccg
     6961 gcacagggat gctggtgctg agcgcctggt gcctgggcat cctcaacagc caccggcgct
     7021 tcctcctttc gtacctggcg cccgtcgtct ggaacctggt catcatcgcc gcgttggtgg
     7081 ccgcgggagg ccgctacgag gaagaggcgt tggtttcggt gctggcctac gccgtggtgc
     7141 tgggcagctt cctccagttc gcggtgcagg tgccctccgt cctcaagttg atgggccgct
     7201 tccgcccgac gctgtcgctg gcggcggagc ctgtgcggca ggtgttgaag aacttcgggc
     7261 cggtcgtgtt ggggcgcggc gtggtccagt tcagcgcctg ggtggacacg gccttcgcgt
     7321 cactcatctc caatcgcgcg ctgtcctcgt tgctctacgc tcagaccatc tacctcatcc
     7381 cggtgagcct ctttgggatg gcggtgtccg cggcggagct gccggagatg tcgcgcgcca
     7441 cgggggaggg cgcggacgtg gccgccaggt tgcgccaacg catcgacgga ggcgcacgcc
     7501 gcatctgctt ctgggtcgtc ccgtcggcgg cggccttcct cttcctgggg gacatggtgg
     7561 cggcggcgtt gctgcagaca gggcgcttcg acgcggcgga ctcgcgctac ctctggtacc
     7621 tgctcatggg ggcggcgctg gggttggtgg cgtccacggt ggggcgcctc tatgcctccg
     7681 ccttctatgc gctgaaggat ccgaagacgc cgctgcgctt cgcggtggtg cgcgtgctgg
     7741 tgggcaccct gggcgcatgg gtgctgggcc tgcggctacc aggatggctt ggcgtgccgc
     7801 agcacctggg cgcgttgggc ctcaccctgg ccagcggcct ggtggcctgg ctggaggcgg
     7861 gactgctgcg ccgcaagctg gtgaagcagc tcgggccggt tggcgtcccg tctggactgc
     7921 tgcctcgctt gtggggcgcc gcggtggtgg ctggcctggt ggcggtgggc atcaaggtgg
     7981 ggctcacctc gctgctcggg ccgatgcccg gtgtgacggc ggagtggggg gggggcttcc
     8041 tgatgccgcc gcgcctgcac cctgttctgg gcttcgcagc ggtggcgctc ccgttcggtg
     8101 ctgtttattt cgggatgtca gcggccctgg gcatcccgga ggcgggaacg gtgttgcgca
     8161 aggtggggcg gaagctgcgt ctggtgcgct aaccacctgg gattgcttgg ctccctgcct
     8221 gccgaacggc cgctatttgg cgttgcaagc atcgggtccg ggatgctctg acggggtgcc
     8281 tccgttatgg acggagcgtt ggcgtcaacg gcatgtgcgt catctggctt cctccggaca
     8341 tggcgcgcgt gtagagtgcg ccgccttttt caccggggcc cggcttctcg ggaagccagg
     8401 gtcgaattcg cttcaggaag gtctcgcagt gagcgacgag aagaacggtt ccaattctgg
     8461 cgggatgggt cccaagaagc cgaaggccac cttcggtgac gtgatgctcg gcatcccttc
     8521 cgggggagct cgcagcgagg gcggtcgggg cgaacagcgt ggcggtggcc gaggcggcga
     8581 gcgcagcggc ggtggccgtg gtgagcgcga cgcccagccg cgtccggcgg gtggtgcgcc
     8641 gcgcgacgag cgggggcccc gtggcgggag cgagcgccgt ggcggaggcg atcgccgtcc
     8701 gtcgggcccc atggtcgtcg tgaagcgggc gtcgggtgcc atcgagacgc gcggcccggt
     8761 gggggatgct cccgccgagg cgaccgcgac ggccgaggag acgaccgccg ccgccgaatc
     8821 ctccgccgcg ccgccggccc ccacgccgcg tccggtcacg ccgacgccgg cgccgaccag
     8881 cgcgctgtat gaggaagttc ccgagtccca gtccttcgcc gagatgttcg aggcgcaggc
     8941 caaggagggt ggaacgccca gccgcaaggg cctgcgcgtg ggcgagaagg tccgtggcac
     9001 catcttccag ctcggcgcgg acaccgcgtt cgtgtccgtg gaaggcgcgg ccaagagcga
     9061 ggcgatgatc gagcttcgcg agctgaagga cgacgagggc atcctgcgtt tcggcgtggg
     9121 tgacaccatc gacgcgcacg tggtggaggt gggggccaag ggcatcctgc tcagccgcgc
     9181 gctggccaag ggcagcgcga acctggccct gctggccgag gcccgcgctt ccggcatgcc
     9241 cgtcgaaggc ctggtgctga gcgtgaacaa gggcggtgtg gaggtcgcca tcggcgacat
     9301 ccgcgcgttc tgccccatca gccagctgga cctgcgctat gtggagaagc cggaccagtt
     9361 catcggcgag aagctccagt tccgcgtgag cgaggtccgc gagcgcaacg tggtgctgtc
     9421 gcgccgctcg ctgctggagg acgagcagcg gcagctggcc gcggagacgc gcaagaacct
     9481 ggcccagggc aagatcgtca agggcaaggt caccggcgtg cgcgacttcg gcgtgttcgt
     9541 ggacctgggc ggcgtggagg gcatgattcc cgtctccgag ctttcgtaca cccgcgtcgg
     9601 tcacccgagc gacgtggtga aggtcggcga tgacgtcgag gtcgagatcc tccgcatgga
     9661 ggccggccag cccaactcgc cggacaagtc caagcagaag gagcgcatca cgttgtccat
     9721 gcgctcgcgc caggaggatc cgttccagaa ggcgctgtcc gaaatcaagg aaggcgaccg
     9781 cctccagggc aaggtcgtcc gcctgcagca gttcggcgcc ttcgtggagc tgcgtccggg
     9841 cgtggatggt ctggtccaca tctccgcgct cagcgaccgc cgcatcgcgc acccgcgcga
     9901 cgtggtgaag gaaggggaga ccatctgggt gtcggtggag aagatcgaca cgcaggagaa
     9961 gcgcatcggc ctgcgccgca tctccgagga agaggcgcag cgtcctcccg aggagcgcac
    10021 ggcgcccgcc gcggaagccg ccgctccggc gcagcccgcc gcgccccgtc ccaaggtggg
    10081 ccaggtcgtc gtgggcaagg tggatcgcat cgagccgtac ggcgtgttcc ttgcgttccc
    10141 gggcggcaag ggcttgctgc ccgcgagcga gacgggcacc gagcgtggca ccgacctgcg
    10201 caagcactac tcgctgggcc aggaggtgaa ggtggccatc ctggacatcg acgcgtccgg
    10261 gaagatccgc ctgtccgtca ccgccgccat ccgcgcggag gagcgcgccg aggtggaggc
    10321 ctggcagaag acgcagcagc cgcagggcgc gggcaagaag ggcttcggca ccttcgcgga
    10381 tctgctcagc aaggcgcgca agtaattccc tgtctccagc gctgatttcg ttgatgtggt
    10441 cagcgctgga gaaaggtgtt gacggtccag gcgagggacg gtacttaccg cctcgcctct
    10501 ctctgccgcg gggtggagca gcctggtagc tcgtcgggct cataacccga aggtcgcagg
    10561 ttcaaatcct gcccccgcaa ctcgttgttt ttcgaaacgt gattggaatc gcagtcgggt
    10621 ttcgagtagt tgagcctcaa gacatgtcgc ggggtggagc agcctggtag ctcgtcgggc
    10681 tcataacccg aaggtcgcag gttcaaatcc tgcccccgca actcaagtcg gcccggtcat
    10741 tctcatggaa tggccgggcc gattcctttt gcacgtctcg acactgcgac cctcctccgg
    10801 catggctgcc ctgcgtccag ccgagcgcgt tgtgttcgct ggtgaaggcg tacccggcac
    10861 cacg
//
