LOCUS       NZ_FMZK01000014        11101 bp    DNA     linear   CON 26-APR-2024
DEFINITION  Streptomyces prasinopilosus strain CGMCC 4.3504, whole genome
            shotgun sequence.
ACCESSION   NZ_FMZK01000014
VERSION     NZ_FMZK01000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces prasinopilosus
  ORGANISM  Streptomyces prasinopilosus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900101585.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 02:19:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,269
            CDSs (total)                      :: 6,190
            Genes (coding)                    :: 5,988
            CDSs (with protein)               :: 5,988
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 202
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 202
            Pseudo Genes (frameshifted)       :: 63 of 202
            Pseudo Genes (incomplete)         :: 175 of 202
            Pseudo Genes (internal stop)      :: 8 of 202
            Pseudo Genes (multiple problems)  :: 40 of 202
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:44:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 169097
            Orig. end                         :: 180198
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FMZK01000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11101
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11101
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11101
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6101](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6101
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(592..606)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.14"
                     /tool="antismash"
     gene            complement(893..1222)
                     /locus_tag="BLS36_RS27195"
                     /old_locus_tag="SAMN05216505_114155"
     CDS             complement(893..1222)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163: rhodanese domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019055193.1"
                     /locus_tag="BLS36_RS27195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_114155"
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_055574613.1"
                     /transl_table=11
                     /translation="MPTVGVSDLKDGDFLLDVRESDEWASGHAEGALHIPISEFVARYG
                     ELTEAAPQDGRVHVICRSGGRSAQVTMYLVQQGVDAVNVDGGMQVWEAAGRPVVTDDGR
                     PGHVL"
     misc_feature    complement(1230..1243)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     18.15"
                     /tool="antismash"
     gene            complement(1296..2150)
                     /locus_tag="BLS36_RS27200"
                     /old_locus_tag="SAMN05216505_114156"
     CDS             complement(1296..2150)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700200.1"
                     /locus_tag="BLS36_RS27200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_114156"
                     /product="hypothetical protein"
                     /protein_id="WP_074995467.1"
                     /transl_table=11
                     /translation="MTTSEGEQGSGGAGAGQAAAGGGAQRPEARLEQAVRAAEQALIEF
                     EIAVDTFRIEVENFSRLHEQRLGPVYARLEELDARILEARAARSGDPEDRRLADEARAG
                     LQPIPGVEELLGGWMDGDGLFPEAAAMLTDQPVRPPERVRPSEEARKLYRDLARKAHPD
                     LAQEEAERRRREGFITRVNAAYARGSEAELRELTAEWAAGPEPADRAPSAVEELYARLE
                     WLAQRKELLTLVAKELEEGAIGSMLRLAPDDPDRLLEEIAEQLRAEVAGREAELAALET
                     PEG"
     gene            complement(2339..3802)
                     /locus_tag="BLS36_RS27205"
                     /old_locus_tag="SAMN05216505_114157"
     CDS             complement(2339..3802)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569153.1"
                     /locus_tag="BLS36_RS27205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_114157"
                     /product="DUF2252 domain-containing protein"
                     /protein_id="WP_074995446.1"
                     /transl_table=11
                     /translation="MSGTGAGGTGTGRAAERAEPGRTAGEARRLPRVPGFAAWPPEGSA
                     KAAGKALRRSVPRKAHSALEPDPSRPDALGAVEESSRGRLPELTPIRVGRMAATPFAFL
                     RGSAGLMGHDLARTPMTRIRAQLCGDAHAANFGLYGDARGGLVIDLNDFDETVWGPWEW
                     DLKRLATSLVLAGREVGADEDTCRKAARDTVGAYRRTMRLLARMPALDAWNAIADEELV
                     SHADAHDLLGTLERVYAKARANTSGRFAAKSTEATEDGGRRFVDAAPVLRRVPDAEALA
                     VASSLEEYVGTLQPDRLPLLARHAVQDVAFRVVGTGSVGTRSYVVLLLDHRGEPLVLQV
                     KEARASALVPHLLTAGFDVPDTGHEGRRVVLGQKRMQVVSDNLLGWTTVEGRPYQVRQF
                     RNRKGSVDPTALSADQVDDYARMTGALLARAHSHSADPRLIAGYCGKSDELDEAMGEFA
                     LHYADRTEADHADLVTAVRSGRIAAEMGV"
     gene            complement(4084..4785)
                     /locus_tag="BLS36_RS27210"
                     /old_locus_tag="SAMN05216505_114158"
     CDS             complement(4084..4785)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092050.1"
                     /locus_tag="BLS36_RS27210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_114158"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_055572819.1"
                     /transl_table=11
                     /translation="MARQERAIRTRRTILDAAAAVFDERGYTSATIGEILGRAGVTKGA
                     LYFHFGSKEELALGIFEEQLAVTLPPQSSKLQELVDSGLVLALRLCTDPLVRASVGLAL
                     DQGALGLDRTPAFRMWIDQITRTLAEAKARGELLPHVEVAETAELLVGSFSGVQLLSQL
                     RCQRQDLEQRVVVLFQHMLPGIAVPAVLARLDISADRGRRALDAGRLEPATGKAAVDAE
                     ADRRAGEVAGR"
     misc_feature    4885..4898
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    4923..4936
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.23"
                     /tool="antismash"
     misc_feature    complement(4929..4938)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 12.94"
                     /tool="antismash"
     gene            5001..6101
                     /locus_tag="BLS36_RS27215"
                     /old_locus_tag="SAMN05216505_114159"
     CDS             5001..6101
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032560.1"
                     /locus_tag="BLS36_RS27215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_114159"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_074995447.1"
                     /sec_met_domain="AfsA (E-value: 5.2e-23, bitscore: 74.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQSGATSTPTGDRTGRRTASAATTGQAAGAPAVTRITPELAHRT
                     RAVDIFPAEWVRRSEHDFRVTAHWPAEHSFFGPVDDLHQDPMIVAETLRQTTMALAHAE
                     FGAPLDTHFVMRDLGISTAPSELLLADAAEPVTVDVVCSEVRRRGRGLSSMRTVMEFHR
                     AGRLVARGTGSMGCTSPAAYRRLRAGRAAGPGTAISPFPPTPPVPRELAGRRRAQDVVL
                     SPSDRPGTWLLRVDTGHPVLFARPNDHVPGMVLFEAARQAATAATGGGVFLPSDLASSF
                     SRYTELDSPCLLETEVLDELDGSGAGDGVLGRGGGDDDGRGAVTVRVVGLQHDEPVFGV
                     VLTSAPHRRAPGPPPAPAPIPVRVLT"
     gene            6098..7066
                     /locus_tag="BLS36_RS27220"
                     /old_locus_tag="SAMN05216505_114160"
     CDS             6098..7066
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (6-134). E-value: 4.6e-10.
                     Score: 30.9. Matches aSDomain:
                     nrpspksdomains_BLS36_RS27220_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991560.1"
                     /locus_tag="BLS36_RS27220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_114160"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_055572943.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.2e-10, bitscore:
                     33.5, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVDLLITGGTGFIGSRITAAARRHPDLRVRSLSRRTPAGSAGPG
                     ATRAAPSPGASPSAGTVPGDLADPASLRGSCAGVDVLVHCASLITGDAESVAAVNDRGT
                     KALVGEAVRSGVRRILYVSTAAVHGRGPFRGVRPGEAPVAPVSATSRSRAAAERHVLAA
                     GGLVLRPHLVYGVGDRWVVPGLVGLLRELSATVADCGALHSMIDVDTLGRVVVAAALSP
                     AHGPGVHYVNHPDPVTTSELLAAVRAQVEQPGGETAVDVTTAYARAAGSPRALHHLGML
                     AVDHWFQDDAFWDGLDCSPGEPFLRAFPHAVPWYRSFVATL"
     aSDomain        6116..6499
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLS36_RS27220_NAD_binding_4.1"
                     /evalue="4.60E-10"
                     /label="BLS36_RS27220_NAD_binding_4.1"
                     /locus_tag="BLS36_RS27220"
                     /protein_end="134"
                     /protein_start="6"
                     /score="30.9"
                     /tool="antismash"
                     /translation="ITGGTGFIGSRITAAARRHPDLRVRSLSRRTPAGSAGPGATRAAP
                     SPGASPSAGTVPGDLADPASLRGSCAGVDVLVHCASLITGDAESVAAVNDRGTKALVGE
                     AVRSGVRRILYVSTAAVHGRGPFR"
     misc_feature    complement(7312..7327)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.24"
                     /tool="antismash"
     tRNA            complement(7526..7609)
                     /anticodon=(pos:complement(176670..176672),aa:Leu,seq:cag)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="BLS36_RS27225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SAMN05216505_114162"
                     /product="tRNA-Leu"
     gene            complement(7526..7609)
                     /locus_tag="BLS36_RS27225"
                     /old_locus_tag="SAMN05216505_114162"
     misc_feature    complement(7792..7801)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(7946..7957)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.19"
                     /tool="antismash"
     gene            8302..9144
                     /locus_tag="BLS36_RS27230"
                     /old_locus_tag="SAMN05216505_114163"
     CDS             8302..9144
                     /GO_function="GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975091.1"
                     /locus_tag="BLS36_RS27230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_114163"
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="WP_074995449.1"
                     /transl_table=11
                     /translation="MGVLKKFEQRLEGLVNGTFAKVFKSEVQPVEIAGALQRECDNNAT
                     IWNRDRTVVPNDFIVELSTPDFERLSPYSGQLGDELAGMVRDYAKQQRYTFMGPIKVHL
                     EKADDLDTGLYRVRSRTLASSSSQAGPGGPPAGAPGGYGYPPAGPAGAPPMPAAPPPGG
                     RPGGYGYPQPAAAHRPPATPASAGRTRYWIEINGNRHQISDATLVLGRSTEADVRIDDP
                     GVSRRHCEIRTGTPSTIQDLGSTNGIVVDGQHTTRATLRDGSRIVVGSTTIIYRQAEG"
     misc_feature    8333..8348
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.32"
                     /tool="antismash"
     gene            9155..9664
                     /locus_tag="BLS36_RS27235"
                     /old_locus_tag="SAMN05216505_114164"
     CDS             9155..9664
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975090.1"
                     /locus_tag="BLS36_RS27235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_114164"
                     /product="FHA domain-containing protein"
                     /protein_id="WP_055573128.1"
                     /transl_table=11
                     /translation="MSELTLTVMRLGFLAVLWLFVIVAVQVIRSDLFGTRVTQRGARRE
                     TARPQQGARQAPPPQRQQSGGGRRGRNAPTKLVVTEGTLTGTTVALQGQTITLGRAHDS
                     TIVLDDDYASSRHARIYPDRDGQWIVEDLGSTNGTYLDRSRLTTPTPIGPGSPIRIGKT
                     VIELRK"
     misc_feature    10844..10862
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.64"
                     /tool="antismash"
ORIGIN
        1 gcgggagcgg catcaccccg gtgctctcca tcgtctcgac ccttctggaa cgcgagcccg
       61 gggcccggtt ctgcctgatc cgcagtgacc gcacggccgc ctcgacgatg ttcctggagg
      121 aggtcgccga cctgaaggac cgcttccccg accggttcca gctggtgccg gtgctctcgc
      181 gggaggagca gcaggcggga ctgccgtccg gacggctcga cgaggagcgg ctcaccgccc
      241 tgctgccggc cctgctgccc gtggggcggg tggacggctg gttcctgtgc ggcccgtacg
      301 ggctggtcga gggggccgca cgggccctgc gcggtctcgg cgtgcagcgt tcccgcatcc
      361 acgaggagat cttccacgtg gacaccggcg caccggtcgt gcccacggcg ccggcccccg
      421 cccacagcac cgtcaccgcc ctgctcgacg gacgcagcgg cacctggccc gtccaggacg
      481 gcgagtccct gctggagacg gtgctgcgca accgctccga cgccccctac gcctgcaagg
      541 gcggggtgtg cggaacgtgc cgcgccttcc tcgtctcggg ccgggtgcgg atggaccgca
      601 acttcgcgct ggagccggag gagaccgacg cgggctacgt gctggcgtgc cagtcgcacc
      661 cgatgacgga gagggtgggg ctggacttcg accggtgacg gccgcaccac cgccctgcca
      721 ccgttccggc ggcgcggtca cgccgtgcgg accgttcccc gccacccccg gctccgctcc
      781 gccccggcat cacgccgggc ggcgggaggc gggggccggg ccggtccccg tccccgtccc
      841 cggcgccctg tccgcggcat ccgccgcgcg ggccgtccgt cccgctccgg tcctacagga
      901 cgtgcccggg ccggccgtcg tcggtgacga cggggcgtcc cgcggcctcc cacacctgca
      961 tgccgccgtc cacgttcacg gcgtcgacgc cctgctggac caggtacatc gtgacctggg
     1021 ccgaacgccc gccggaacgg cagatcacgt gcacccggcc gtcctgcgga gccgcctcgg
     1081 tcagctcgcc gtagcgggcg acgaactcac tgatcgggat gtgcagtgcc ccttcggcat
     1141 gaccggacgc ccactcgtcg ctttcgcgga cgtccagcag gaagtcgccg tccttgaggt
     1201 ccgagacccc gaccgtgggc accccagtcg caaaactcat gcccccgacg ctaccggaag
     1261 ccccggcccg gggcccacgc ccgccgggcc ggacctcagc cctcaggggt ctccagggcc
     1321 gccagctccg cctcgcgccc ggccacctcg gcgcgcagct gctcggcgat ctcctccagg
     1381 agacggtccg ggtcgtccgg ggccagccgg agcatcgagc cgatggcgcc ctcctcgagc
     1441 tccttcgcga ccagcgtgag cagttccttg cgctgggcga gccattcgag ccgggcgtag
     1501 agctcctcga cggcgctcgg ggcccggtcc gccggctccg ggcccgccgc ccattccgcg
     1561 gtgagttccc gcagctcggc ctcgctgccg cgggcgtagg cggcgttgac gcgggtgatg
     1621 aacccctcgc ggcgacggcg ttccgcctcc tcctgcgcca ggtccgggtg ggccttgcgg
     1681 gcgaggtcgc ggtagagctt gcgtgcctct tcgctgggcc gcacccgctc cgggggccgg
     1741 acgggctggt cggtgagcat ggccgcggcc tccgggaaga gaccgtcgcc gtccatccag
     1801 ccgcccagca gttcctccac gccggggatc ggctgcagcc cggcgcgcgc ctcgtcggcc
     1861 agccggcggt cctcgggatc accgctgcgg gcggccctgg cctccaggat gcgggcgtcc
     1921 agctcctcga ggcgtgcgta cacggggccg agccgctgct cgtgcagccg ggagaagttc
     1981 tcgacctcga tgcggaaggt gtccaccgcg atctcgaact cgatcagcgc ctgttccgcg
     2041 gcccgcacgg cctgctccaa ccgcgcctcg ggccgctgcg caccaccgcc ggccgccgcc
     2101 tgccccgcac cggcgccgcc gctgccctgc tcaccctccg aggtcgtcac ccggacagcg
     2161 taggccacca ccgccaccgc cgaatccccc caccctgccc ccggcaccgc ccccttcgcc
     2221 cccggcagcc gacccggccg gcctctcccc ccggccgggt cggggaccgg ccggccccgg
     2281 accggtcgcc cgccggttcc cgggcggcgg agagcccccg gaggaacagg agcggtgctc
     2341 agacccccat ctccgccgcg atccggcccg agcggaccgc cgtgaccagg tccgcgtggt
     2401 ccgcctccgt gcggtcggcg tagtggaggg cgaactctcc catcgcctcg tcgagttcgt
     2461 cgctcttgcc gcagtagccc gcgatcaggc gggggtcggc gctgtgggag tgggcgcggg
     2521 ccaggagggc tccggtcatc cgggcgtagt cgtcgacctg gtcggcggag agggccgtgg
     2581 ggtcgacgct gcccttgcgg ttgcggaact gccgcacctg gtagggccgc ccctcgacgg
     2641 tcgtccagcc cagcaggttg tcgctgacca cctgcatccg cttctggccc aggaccacgc
     2701 gccggccctc gtggccggtg tcggggacgt cgaagccggc cgtcagcagg tgcggcacca
     2761 gcgccgaggc gcgggcctcc ttcacctgga ggacgagcgg ttcgccccgg tggtcgagga
     2821 gcagcacgac gtacgagcgg gtgcccacgc tgcccgtgcc gacgacccgg aacgccacgt
     2881 cctgcaccgc gtgccgggcc agcagcggaa gccggtcggg ctggagggtg ccgacgtact
     2941 cctccaggga ggaggccacc gccagggcct cggcgtccgg gacgcgacgc agcaccgggg
     3001 cggcgtcgac gaagcgacgg ccgccgtcct ccgtcgcctc ggtggacttg gccgcgaaac
     3061 ggccgctggt gttggcgcgg gccttcgcgt agacccgctc cagcgtgccg aggagatcgt
     3121 gcgcgtcggc gtgggagacc agttcctcgt cggcgatggc gttccacgcg tccagcgccg
     3181 gcatcctcgc cagcagccgc atcgtgcgac ggtaggcgcc caccgtgtca cgggcggcct
     3241 tgcggcaggt gtcctcgtcc gcgccgacct cccgccccgc gagcaccagc gaggtggcga
     3301 gccgcttgag gtcccactcc cagggccccc acaccgtctc gtcgaagtcg ttcaggtcga
     3361 tgaccagccc gccgcgcgcg tcaccgtaca gaccgaagtt cgccgcgtgc gcgtcgccgc
     3421 agagctgcgc gcggatccgc gtcatggggg tgcgggccag gtcgtggccc atcaggcccg
     3481 ccgatccgcg caggaaggcg aagggcgtcg ccgccatcct gcccacccgt atcggcgtca
     3541 gctcgggcag ccggccgcgg ctggactcct cgaccgcacc gagcgcgtcg gggcgggagg
     3601 gatccggctc gagagcggag tgcgccttgc gcggcaccga ccggcgcaga gcctttcccg
     3661 ccgccttcgc cgagccctcg ggcggccacg ccgcgaagcc cggtacgcgc ggcagccgcc
     3721 gcgcctctcc ggcggtgcgg cccggctccg cccgctccgc cgccctgccc gtcccggtcc
     3781 cgccggcacc cgttccgctc atcacaaccg cctcccccgt acgtccacac gtacggacac
     3841 gtcggccgcg ccgcacacga acggccaccg accgccggcc cgcctcgatg ccggaacgcg
     3901 cgtcggcgca ggccatgaca tcgaccgtgc cgacggtaca accggtggcc gaaaagcgtc
     3961 aggcccggtg gacgactccg gggccgtgga cacctccgtc acccgtccgc gcccctgccc
     4021 cgtcccccac cccgtggccc ccacccggcc cccgcccgag ccacggcgac cgcccgccgg
     4081 gtcctagcgt cccgccacct cgccggcccg ccggtccgcc tccgcgtcga cggccgcctt
     4141 ccccgtggcc ggttccagtc gccccgcgtc gagggcccgt cggccgcggt ccgccgagat
     4201 gtccagccgg gccagcaccg ccggcaccgc gatgcccggc agcatgtgct ggaacagcac
     4261 cacgacccgc tgctccagat cctgccgctg acagcgcagc tgcgagagga gctgcactcc
     4321 cgagaagctc ccgaccagca gttccgccgt ctccgccacc tcgacgtgcg gcagcaactc
     4381 gcccctcgcc ttcgcctcgg ccagggtccg ggtgatctgg tcgatccaca tccggaacgc
     4441 cggagtccgg tccagtccca acgctccctg gtccagggcc agtccgacgc tggcgcgcac
     4501 cagcgggtcc gtgcacagcc gcagggccag caccagaccg gagtcgacga gttcttggag
     4561 cttgctggac tggggcggca gggtcaccgc cagctgctcc tcgaagatgc cgagcgccag
     4621 ctcctccttg gagccgaagt ggaagtacag cgcgcccttg gtcaccccgg cccggcccag
     4681 gatctcgccg atggtcgccg aggtgtaacc gcgttcgtcg aagaccgccg cggccgcgtc
     4741 cagaatggtc cgccgtgtgc ggatcgcgcg ctcctgtcgc gccatcgggt gcctcctgat
     4801 ctccgtggat ctcgtggatc gccgtgaagt agccatacgg ccgccgtgcg gtcggcgtgc
     4861 cgccgtacca cgatgccgcg cgggcgcatg ggcttgcggg ctcgtcccca acggtgtgga
     4921 tcacccttga aaaaaaccgg ctcgtccgta tcttagtgcg aagtcgaccg ggtccaggcc
     4981 ttctctccgg agggttcctc atgccgcagt caggtgccac cagcacgccc acgggggacc
     5041 gaaccggacg gcggaccgcg tccgcggcca ccaccggaca ggccgccgga gcccccgccg
     5101 tcacccggat caccccggaa ctggcccacc ggacccgcgc ggtggacatc ttcccggcgg
     5161 aatgggtccg ccggtccgaa cacgacttcc gcgtcaccgc gcactggccc gccgagcact
     5221 ccttcttcgg ccccgtggac gacctccacc aggatccgat gatcgtcgcc gagacgctgc
     5281 gccagaccac gatggccctc gcccacgccg agttcggcgc ccccctcgac acccacttcg
     5341 tcatgcggga cctggggatc tccaccgccc cctccgaact gctgctcgcg gacgccgcgg
     5401 agccggtcac cgtcgacgtc gtctgctccg aggtacgccg ccggggccgc ggtctgagca
     5461 gcatgcggac cgtcatggag ttccaccggg cgggacggct cgtcgcccgg ggcactggca
     5521 gcatggggtg cacctcgccg gccgcgtacc gccgtctgcg cgccgggcgg gcggccggtc
     5581 cgggtaccgc gatatccccc ttccccccca cccctcccgt ccctcgcgaa ctggccggcc
     5641 gccgccgcgc gcaggacgtc gtcctctccc cctcggaccg ccccggcacc tggctcctgc
     5701 gcgtcgacac cgggcacccc gtcctcttcg cccggcccaa cgaccacgtc cccggcatgg
     5761 tcctgttcga ggcggcccgc caggccgcga cggcggcgac cggcggaggt gtcttcctcc
     5821 cctccgacct ggcctcctcc ttcagccgct acaccgaact ggacagcccc tgcctcctcg
     5881 agaccgaggt cctggacgaa ctcgacggga gcggagccgg cgacggggtc ctgggccgcg
     5941 gcgggggaga cgacgacggc agaggcgcgg tgaccgtacg ggtcgtcggc ctgcagcacg
     6001 acgaacccgt cttcggcgtc gtcctcacct cggcgcccca ccgccgggct ccggggccgc
     6061 ccccggcccc ggccccgatc ccggtacggg tgctgacgtg acggtggacc tcctgatcac
     6121 gggcggcacc ggcttcatcg gcagccggat caccgccgcg gcacgccggc acccggacct
     6181 tcgcgtccgg tcgctctccc gccgtacgcc cgccgggagc gccgggcccg gagccacgcg
     6241 ggcggctccc tccccgggcg cgtccccgtc cgccggaacc gttcccggcg acctcgccga
     6301 tcccgcctcc ctgcgcggca gttgcgccgg cgtcgacgtc ctcgtccact gcgcctcgct
     6361 catcaccggc gacgcggagt ccgtcgcggc ggtcaacgac cggggcacga aggccctggt
     6421 gggggaggcg gtgcgcagcg gggtgcgccg catcctgtac gtgagcaccg ccgccgtcca
     6481 cggcaggggc cccttccgcg gcgtacggcc cggcgaggcc cccgtcgccc cggtctccgc
     6541 caccagccgc agccgcgccg cggccgaacg gcacgtgctc gcggcgggcg gcctggtgct
     6601 gcggccccac ctggtgtacg gcgtgggcga ccgctgggtg gtccccggac tggtggggct
     6661 cctgagggag ttgtcggcga ccgtggcgga ctgcggggcc ctgcactcga tgatcgacgt
     6721 ggacaccctg ggccgcgtgg tcgtcgccgc ggcgctctcc ccggcccacg gccccggcgt
     6781 ccactacgtc aaccatccgg acccggtcac cacgtcggag ctgctcgcgg ccgtccgcgc
     6841 ccaggtggaa cagcccggcg gggagacggc ggtcgacgtc acgaccgcct acgcgcgggc
     6901 cgccggctcc ccccgcgccc tgcaccacct cggcatgctc gccgtcgacc actggttcca
     6961 ggacgacgcc ttctgggacg gcctggactg ctccccgggt gagcccttcc tccgggcctt
     7021 cccgcacgcg gttccctggt accggtcgtt cgtcgccacc ctctgaccgg ccgtcccccc
     7081 ggccaccgca ccggcgcccg ccaccgcctc gcgcgcccgc tcccacaccc ctgtgcgctc
     7141 cccgggaccg aaccgacccg tcggattcgt tcccgcggtg agcacgcgac gcccccgcgc
     7201 gcggacgcgg cgtcgacccg ccgcaccgcc ggccccgccg taccggcccg cgttcgactg
     7261 cggagcgtac cgacacccgt gcggtcggtc accgaacatc ggaaaggatc atgagatgtt
     7321 cctcacacgg gcggccccct cgccgccccg ctcctccccc tgcccccggc cccggcccgt
     7381 gccccgccgc cctccggacc ggagcggagc ggggtggagc ggggtggagc ggacccgacc
     7441 cgggcgaggc cgggccgcac ccgggcccgg gcccgggcat gcgaaacggg cggcatcctc
     7501 aatggatgcc gcccgtccga cggtgtgcgc gaggggggag ttgaaccccc acgcccttgc
     7561 gggcactgga acctgaatcc agcgcgtctg cctattccgc cacccgcgca ttgggtgtgt
     7621 ctccaggtcc ttccccgtgg gtccggcgcc tttcgactcc tgaacattag cacgccgacg
     7681 ggggtggact cacatccctt tcccccgccc cggcacccgc ccgcgccgag cggcaccatc
     7741 gcccgtgagc aggccgcgag cagcccgcgg cccgcccggg ggctgctcgc cggccgcctt
     7801 cgaacggcca cggacgaccg gaggggccgc accgtcccgt atcgccccgt atgcctcccg
     7861 ccgctccgga cgcacacatg ccgacgagca gcgcatatcc gccccactcg ccccacccgt
     7921 ccacgcgaac cggcgagaac cgaacggaac cgctttcacg tatcaacctc gtaccggtgg
     7981 cgcccgtctc ccaggtgccg ggcggagtcc acagccgggt gcgggacact ggtctgcggc
     8041 cccctctacg atccatggcg gagggctcgc acgagcacgg cccgaggagt tcgcaggcga
     8101 gcaccgaagg gcctccgtca ggcgccgaca cccgtcgggc cgggcgggca gggggaacca
     8161 gccgatcgac cgaggcgtgg atacgatcag taagcagtac cagttatgcg gtgcacggga
     8221 cagcgcaccg ggcagtagac gcggtagacg cggcagaaga cagggcagta cacaggacgg
     8281 caacgacgga ggaggtgcca catgggagtc ctgaagaagt tcgagcagcg actcgaaggc
     8341 ctggtcaacg gcacctttgc caaggtgttc aagtccgagg tgcagcccgt ggagatcgcg
     8401 ggagcgctcc agcgggaatg cgacaacaac gcgaccatct ggaaccgcga tcgcaccgtt
     8461 gtccccaacg acttcatcgt cgagctgagc acccccgact tcgagcggct cagcccgtac
     8521 tccgggcagc tcggcgacga actcgccggc atggtgcgcg actacgccaa gcagcagcgc
     8581 tacaccttca tgggccccat caaggtgcac ctggagaagg cggacgacct cgacaccggt
     8641 ctgtaccggg tgcgcagccg tacgctcgcc tcctccagca gccaggcggg ccccggcggc
     8701 ccgcccgccg gcgcccccgg cggctacggc tatccgccgg ccgggcccgc gggcgccccg
     8761 cccatgccgg ccgcgccgcc gcccggcggc cgtcccggcg gttacggcta cccgcagccc
     8821 gcggccgccc accggccccc cgccaccccc gcgtccgccg ggcgcacccg gtactggatc
     8881 gagatcaacg gcaaccgcca ccagatctcc gacgccacgc tcgtactggg ccgcagcacc
     8941 gaggccgacg tgcggatcga cgaccccggc gtctcccgcc ggcactgcga gatccggacc
     9001 ggaacgccct cgacgatcca ggacctcgga tccaccaacg gcatcgtggt ggacggacag
     9061 cacaccaccc gcgctacgct ccgcgacggc tcgcggatcg tcgtgggcag caccaccatc
     9121 atttaccggc aagccgaagg gtgaagcggg ggcaatgtca gagctgaccc tcacggtcat
     9181 gcggctgggt tttctggccg ttctgtggct gttcgtgatc gtggccgtgc aggtcatccg
     9241 cagtgacctg ttcggcacgc gggtcaccca gcgcggagcg cgccgagaga ccgcccggcc
     9301 gcaacagggc gcccggcagg caccaccgcc gcagcgccag cagtccgggg gcggccgccg
     9361 cggccgcaac gcccccacca aactggtcgt gaccgagggc accctcacgg gcaccaccgt
     9421 cgcgctccag ggccagacca tcacactggg ccgggcgcac gacagcacca tcgtgctgga
     9481 cgacgactac gcctccagcc gccacgcccg gatctacccc gaccgcgacg gccagtggat
     9541 cgtcgaggac ctgggttcca cgaacggcac ctatctggac cggtccaggc tgacgacccc
     9601 cacaccgatc ggaccgggtt caccgatccg catcggcaag accgtcatcg agctgcggaa
     9661 gtagtacgac aatgagcgag cggagcgagc acgcaggggg ggtccccacc ccgggccccg
     9721 gcgcgctccc gaccggaggg tgggcagtgt ggctcgacac gaccggctgt acccggagcc
     9781 gacgggcgag gtgcgcatga gtctgtccct gcgcttcgcc gccggatcgc acaaggggat
     9841 gatccgggag ggcaacgagg actccggtta cgccggtccc cgcctgctcg ccatcgccga
     9901 cggcatgggc ggcgccgcgg ccggcgaggt cgcctcctcc gaggccatct ccaccatcgt
     9961 ctcgctcgac gacgacgtcc ccggctccga cctgctcacc tccctcggct cggccgtgca
    10021 gcgcgccaac gaccagctgc gcatgatggt cgaggaggac ccccagctcg agggcatggg
    10081 caccaccctg accgcgctgc tgtggaccgg ccagcgcctc ggcatggtcc acgtcggcga
    10141 ctccagggcc tacctgctgc gcgacggcgt cctcacccag atcacccagg accacacctg
    10201 ggtgcagcgg ctggtggacg agggccggat caccgaggag gaggcgacca cccacccgca
    10261 gcggtccctg ctcatgcggg ccctcggcag cggcgagcac gtcgagcccg acctgtcgat
    10321 ccgcgaggtc cgggcgggcg accggtacct gatctgctcc gacggcctgt ccggcgtggt
    10381 ctcccaccag accctggaag aggccctcgc cggctaccag ggccccgagg acaccgtgca
    10441 gaccctgatc gagctggcac tgcgcggcgg cggcccggac aacatcaccg tgatcatcgc
    10501 cgacgtcctc gacctcgaca ccggtgacac cctggccggc cggctgtccg acaccccggt
    10561 cgtcgtcggc gccgtcgccg agaaccagaa ccaggccggc gacaacagcg ccatgcagac
    10621 cccggccggc cgcgccgccg gactcggccg caggcgcggc cagggcggcg gcgagttcgg
    10681 cccgcccggc agcggcgacg tcaccggctt catccagacc gacggcctcg actacgacga
    10741 gggcgacttc gtcaagcccc gcaagaaccg ccggtggctc aaacgctcct tcttcagcct
    10801 gctcaccctc gccgtcatcg gcggcggcgt gtacagcggc tggcgctgga cgcagaccca
    10861 gtactacgtc ggcgccaacg acgaacacgt cgcgctctac cagggcatca accaggacct
    10921 ggcctgggtc tcgctctcga aggtcgagaa ggaccacccc gagatcgaac tcaagtacct
    10981 gccgccctac cagcggaagc tcgtcgaggc gaccatcgtc gagagcgacc tgcacggcgc
    11041 ccggtcgaag atcgacgaac tcgcggtcca ggcctccgcc tgcaggaagc aggccgagcg
    11101 a
//
