LOCUS       NZ_FMZK01000002        10987 bp    DNA     linear   CON 26-APR-2024
DEFINITION  Streptomyces prasinopilosus strain CGMCC 4.3504, whole genome
            shotgun sequence.
ACCESSION   NZ_FMZK01000002
VERSION     NZ_FMZK01000002.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces prasinopilosus
  ORGANISM  Streptomyces prasinopilosus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900101585.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 02:19:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,269
            CDSs (total)                      :: 6,190
            Genes (coding)                    :: 5,988
            CDSs (with protein)               :: 5,988
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 202
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 202
            Pseudo Genes (frameshifted)       :: 63 of 202
            Pseudo Genes (incomplete)         :: 175 of 202
            Pseudo Genes (internal stop)      :: 8 of 202
            Pseudo Genes (multiple problems)  :: 40 of 202
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:44:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 47208
            Orig. end                         :: 58195
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FMZK01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10987
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10987
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10987
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5987](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5987
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    102..116
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.27"
                     /tool="antismash"
     misc_feature    complement(112..127)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.98"
                     /tool="antismash"
     gene            complement(492..1169)
                     /locus_tag="BLS36_RS06115"
                     /old_locus_tag="SAMN05216505_10235"
     CDS             complement(492..1169)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993738.1"
                     /locus_tag="BLS36_RS06115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_10235"
                     /product="DUF3000 domain-containing protein"
                     /protein_id="WP_074993821.1"
                     /transl_table=11
                     /translation="MAAAQGRLSDGTGGMDEAKDTDAELGDGGATPPPPFRTAVEALRG
                     ARLRPQIEVEVTPAPKRLAPYAYALEAAVVDGDQDLADGRLVLLHDPAGHDAWRGTFRL
                     VTLVRAELEPEMAADPLLPEVCWSWLTGALAARGLTYGEPSGTVTRASSHYFGGLSERP
                     AASQIEIRASWTPGEGAGGTPDAATHLMSWCDLLAQVAGLPPTAPGDASVVTLPQRRGP
                     QSR"
     gene            1349..2416
                     /gene="hemE"
                     /locus_tag="BLS36_RS06120"
                     /old_locus_tag="SAMN05216505_10236"
     CDS             1349..2416
                     /EC_number="4.1.1.37"
                     /GO_function="GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="hemE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564423.1"
                     /locus_tag="BLS36_RS06120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_10236"
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="WP_055572793.1"
                     /transl_table=11
                     /translation="MSANVSPRGKQPTQTYDSAFLKACRREPVPHTPVWFMRQAGRSLP
                     EYRKVREGIAMLDSCMRPDLVAEITLQPVRRHNVDAAIYFSDIVVPLKAIGIDLDIKPG
                     IGPVVEQPFRTRADLARLRDLTPEDVSYVTEAYGLLTAELGSTPLIGFAGAPFTLASYL
                     VEGGPSRTYENAKAMMYGDPELWADLLDRLADITAAFLAVQIEAGASAVQLFDSWAGAL
                     SPADYRRSVMSASAKVLDAVSGYGVPRIHFGVGTGELLGLMGEAGADVVGVDWRVPLEE
                     AARRVGPGKALQGNLDPTVLFAGTEAVEAKTREVLAAAEGLEGHVFNLGHGVMPNTDAD
                     ALTRLVDHVHTATAR"
     misc_feature    complement(1353..1365)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 18.88"
                     /tool="antismash"
     misc_feature    3067..3080
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.39"
                     /tool="antismash"
     misc_feature    complement(3068..3081)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.35"
                     /tool="antismash"
     misc_feature    3068..3082
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.32"
                     /tool="antismash"
     misc_feature    complement(3069..3082)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.15"
                     /tool="antismash"
     gene            3237..3869
                     /locus_tag="BLS36_RS06125"
                     /old_locus_tag="SAMN05216505_10237"
     CDS             3237..3869
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018844960.1"
                     /locus_tag="BLS36_RS06125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_10237"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_055574999.1"
                     /transl_table=11
                     /translation="MAKQERSEHTQGKLIVAAAEQFALRGFLKATLGDVSREAGVTKGA
                     LFFHFATKDDLAAAVQRRGRDILETMIADMSGSEESCLQIVVDALHALSRMLRSDPFVR
                     AGVRIARERTAESPVPVDFYALWLGTLGEVLERARKNGELAADVADASARALVTTVVCG
                     VETLAWMGVSEDEGDKWLGCLLELVLPSIASADGLRRVRIPGLRGTP"
     gene            complement(4115..4780)
                     /locus_tag="BLS36_RS06130"
                     /old_locus_tag="SAMN05216505_10238"
     CDS             complement(4115..4780)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009191158.1"
                     /locus_tag="BLS36_RS06130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_10238"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_055574998.1"
                     /transl_table=11
                     /translation="MATGSRTRQERAEATRRAILDGAATAFDAAGFGSTSLTDIAQHAG
                     VTKGAMYFHFQSKEALARELVAEQFELLPGGAESELRALQRAIDLTHRMAHGLRSSARL
                     RAGIRLVIERGSFSTPDPSPYNQWIDSVRAMLANAQGAGDLKSEVDVGDLSTYIVGAFT
                     GVQITSQVRTAREDLHERVADLWTWLLPGVVPARRLARFAPAGSEELWAALEDGAATG"
     misc_feature    complement(4996..5009)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.22"
                     /tool="antismash"
     gene            5001..5987
                     /locus_tag="BLS36_RS06135"
                     /old_locus_tag="SAMN05216505_10239"
     CDS             5001..5987
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433638.1"
                     /locus_tag="BLS36_RS06135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_10239"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_079170664.1"
                     /sec_met_domain="AfsA (E-value: 4.8e-21, bitscore: 70.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNALLQADRPYFGKPEECGPELFEQTVPRTLVHRAAVSEVLLTGW
                     QTVHDEHRLGAQWSRAHSYYGSVDGRWHDPMLFAETIRQACLLLAHEALDVPLGFGFLT
                     ESTAFEVDEERLRLSRPAHPAHVVLDMRLSHIKRRAGVVSSYAYDVVAHRDGERLGSGR
                     LRGKCTSQAVYRRLRGERIGAGPPHTAIAPVDPRLVGRDDESDVVLGASAGAGAHPLRI
                     VADHPVLFDHPVDHVPGMVLMEAARQAGLLALGAPHGLLVACEAQFRRYVEFHIPCLVA
                     TDEPEHEGGGRRTVTVRFLQDGSEAGSCRVGILTTAGEADGAATAQH"
     misc_feature    5346..5348
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            <6111..>6229
                     /locus_tag="BLS36_RS32200"
                     /pseudo=""
     CDS             6111..>6229
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558180.1"
                     /locus_tag="BLS36_RS32200"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="IS5/IS1182 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRIPGRGGHPGTTAPMRDPDGPGAVRPAGSTSPVRAASA"
     gene            complement(6244..6996)
                     /locus_tag="BLS36_RS06145"
                     /old_locus_tag="SAMN05216505_10240"
     CDS             complement(6244..6996)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020139894.1"
                     /locus_tag="BLS36_RS06145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_10240"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_079170665.1"
                     /transl_table=11
                     /translation="MEEGRAPAAPDAGADDAVELPAVDALDWPSLTTALDAEGVAVTPP
                     LLSPRACEEVRALFDRDEVFRSTVDMGRHRFGSGRYRYFAAPLPPVVAALRRQLYPPLA
                     RIANQWAERLDRPRFPQTHAGLTAACAAAGQLRPTPLLLRYGPGDYNCLHQDVYGELTF
                     PLQVAVMLDRPDVDFTGGESVFVEQRPRAQSRPVVKRPGQGQGLIFAVHDRPVPSARGW
                     SRFMLRHGVSAVHDGQRHVLGVIFHDAP"
     gene            complement(7097..7642)
                     /locus_tag="BLS36_RS06150"
                     /old_locus_tag="SAMN05216505_10241"
     CDS             complement(7097..7642)
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473727.1"
                     /locus_tag="BLS36_RS06150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_10241"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_055572750.1"
                     /transl_table=11
                     /translation="MSTPPTAALAPHRTTLGPLTLAATDDALVLCAFDDREQALARLGR
                     GGLTAVDADAATADQRRILEEAGRQLDAYLEGRSRAFTVRMDLRLATPFSRTTVERLDG
                     LLPYGATGTYAELAAALGRPRAARAVGTALGANPLCVVLPCHRVVGSGGRLTGYAGGVA
                     AKRFLLDLESGRTSPASL"
     gene            complement(7678..8478)
                     /locus_tag="BLS36_RS06155"
                     /old_locus_tag="SAMN05216505_10242"
     CDS             complement(7678..8478)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070858.1"
                     /locus_tag="BLS36_RS06155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_10242"
                     /product="MGMT family protein"
                     /protein_id="WP_055572749.1"
                     /transl_table=11
                     /translation="MSEGNGDAVTRRLPDLAAGAPEDFALRVLRRAGLSREHYDTYVRL
                     DTAAGGLFVAASPQTVTGACLDSAGLTATGFEELHRARTGRSAIPGGRPLPGLLPALRT
                     GRARRLAIDLSGLPEGQRAILEAVRSIPPGQLRPLSWIAREADAGSPPPVSDVLGALTA
                     NPVAVLIPCHRVTHDNGSPCDAAYLPKAGEALRRAEHIDMRRVAELSRAGAVFLGSDTT
                     FIYCHPTCAHARRITPGHRVHFPTARDAREAGYRACKSCRPLTV"
     gene            complement(8475..9065)
                     /locus_tag="BLS36_RS06160"
                     /old_locus_tag="SAMN05216505_10243"
     CDS             complement(8475..9065)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070857.1"
                     /locus_tag="BLS36_RS06160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_10243"
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_055572748.1"
                     /transl_table=11
                     /translation="MDDKAEQLTDLLAVHLEAGFTELVRVHAGAVHAFLYRVSGSAAEA
                     DDLGQDTFLRAYTALREYAPARRRELRPRAWLMTIAANVWRNHVRTRTRRPVTAGLPVE
                     ESMDVWPDHSPGPDERAATAEDRSRLVTALSALPEHHRIPVVLHHVIGMSYTEVAEVQG
                     CAVGTAKAQVSRGLAGLRRLLEPEPTVLKEVTM"
     misc_feature    complement(8655..8657)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(9118..9846)
                     /locus_tag="BLS36_RS06165"
                     /old_locus_tag="SAMN05216505_10244"
     CDS             complement(9118..9846)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070856.1"
                     /locus_tag="BLS36_RS06165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_10244"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_240503143.1"
                     /transl_table=11
                     /translation="MNLVPSAPKPRQLLAFLMLSTPQMVRAGDCITELWGSTPPKSAMS
                     TLQTYVLHIRQALRGPRGDLSHLLVTRNQGYQLALETTDFDRFRFTDLTRRGRVAAAAG
                     DHRTASEMFTLALAQWRGPALSDVRTGPLSSPHLAELNETRQGVLEQRIEADLALGKHR
                     ELVDELSDLTARHPTHENVHAQLMVALYRSGERKRAGDVFDGLRRTLGDSLGIEPVPRM
                     RSLYQAILVGSSALDAPVVG"
     misc_feature    9845..9860
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.97"
                     /tool="antismash"
     misc_feature    9885..9898
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.08"
                     /tool="antismash"
     misc_feature    complement(9925..9940)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: medium, score: 24.12"
                     /tool="antismash"
     misc_feature    complement(9935..9951)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.58"
                     /tool="antismash"
     misc_feature    complement(9940..9953)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.88"
                     /tool="antismash"
     misc_feature    9942..9962
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 20.5"
                     /tool="antismash"
     misc_feature    complement(10042..10055)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.16"
                     /tool="antismash"
     misc_feature    10042..10056
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.62"
                     /tool="antismash"
     misc_feature    complement(10043..10056)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.98"
                     /tool="antismash"
     misc_feature    10043..10057
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.56"
                     /tool="antismash"
     misc_feature    10179..10193
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.1"
                     /tool="antismash"
ORIGIN
        1 acaccaggcc tcctggggtg gcggcgggac ggggccggct cgtgggggaa ccgggctcaa
       61 ccgtgctttc aaggtcacag tcgtcttcgg caacaaacac ggtctccttt agagaatgat
      121 cacgatccgg tgagtaacaa tccgtgcaat tcggacgcgc ggtcgatcat tcgaagtccg
      181 aacggtttcg gtccgcgtcg caacacttcc gaaagtggcc gtatcgacaa agagatcatc
      241 aaaaaggtcg ccaaagagat gccgggaaga tcgacagggg gagcggccgg gggaacggcg
      301 ggaggaccgg tccgcgaacg ctccggtcac cggccccggg agggctccgg cgagcggtcc
      361 ggcgaccggc ccgggaacga cgaagggacc ggcccgaggg ccggtccgga ggatcgttgt
      421 acgtccgggg aggggcgcgg cggggtccgc gggttccccg tgcccgcggc gggctcagcg
      481 gcgcggcggc gtcagcgcga ctgcggccct ctgcgctgcg gcagcgtcac caccgacgcg
      541 tcgcccggag cggtcggcgg caacccggcg acctgcgcca gcaggtcgca ccacgacatc
      601 aggtgcgtgg ccgcgtccgg ggtcccgccc gcgccctccc ccggcgtcca cgaggcgcgg
      661 atctcgatct gcgaggcggc ggggcgctcc gacagcccgc cgaagtagtg cgaactcgcc
      721 cgggtgaccg tgccgctcgg ctcgccgtac gtcaggccgc gtgccgcgag cgcaccggtc
      781 agccaggacc agcacacctc cggaagcagc ggatccgccg ccatctccgg ctccagttcc
      841 gcacgcacca gcgtcaccag ccggaaggtg ccccgccagg cgtcgtgccc ggccgggtcg
      901 tgcagcagca ccagccggcc gtcggccagg tcctggtcgc cgtccacgac cgccgcctcc
      961 agcgcgtacg cgtacggagc gagccgcttg ggtgccgggg tgacctccac ctcgatctgc
     1021 ggccggagcc gtgcgccccg caacgcctcg acggcggtcc ggaagggcgg cgggggtgtc
     1081 gcgcccccgt ccccgagctc cgcatcggtg tccttcgctt cgtccattcc gcccgtgccg
     1141 tccgacagtc gtccctgagc cgcagccatg cggggaaggt taagccgaag cgggctccgg
     1201 cgcggggagg gacacccggg gcggacaacg gtggccggat cgtgtcgcac cggcgtggcg
     1261 cgggccgcga cgcccggcgt ggcgcggggc gaggtgcccg ggtgacctgc ggtgccgggc
     1321 cgtcggccga ccgtgcgaga ctggccgggt gagtgccaat gtcagccccc ggggcaagca
     1381 gccgacccag acgtacgaca gtgccttcct caaggcgtgc cggcgtgagc cggtgccgca
     1441 cactccggtg tggttcatgc ggcaggcggg ccgttcgttg cccgagtacc gcaaggtgcg
     1501 cgagggcatc gcgatgctcg actcctgcat gcgccccgac ctcgtcgccg agatcacgct
     1561 ccagccggtg cgccggcaca acgtcgacgc ggcgatctac ttcagcgaca tcgtcgtccc
     1621 gctcaaggcc atcgggatcg acctcgacat caagcccggt atcggccccg tcgtcgagca
     1681 gcccttccgc acccgcgccg acctggcccg gctgcgcgac ctcaccccgg aggacgtctc
     1741 ctacgtcacc gaggcgtacg gcctgctgac cgccgagctg ggctccaccc cgctgatcgg
     1801 cttcgcgggc gccccgttca ccctcgccag ctacctcgtc gagggcggcc cgtcccgcac
     1861 gtacgagaac gccaaggcga tgatgtacgg cgacccggaa ctctgggccg acctcctcga
     1921 ccgcctcgcg gacatcacgg ccgccttcct cgccgtgcag atcgaggccg gcgcctccgc
     1981 ggtccagctc ttcgactcct gggccggcgc cctctccccg gccgactacc ggcgctcggt
     2041 gatgtccgcc tcggccaagg tcctcgacgc ggtgtccggt tacggcgtcc cccgcatcca
     2101 cttcggtgtc ggcaccggcg aactcctcgg cctgatgggc gaggccggcg cggacgtcgt
     2161 cggcgtcgac tggcgcgtcc cgctggagga ggccgcccgc cgggtcggcc ccggcaaggc
     2221 gctccagggc aacctcgacc cgaccgtgct gttcgcgggc acggaggcgg tggaggcgaa
     2281 gacccgcgag gtgctggccg cggccgaggg cctcgagggc cacgtcttca acctgggcca
     2341 cggcgtcatg ccgaacaccg acgccgacgc gctgacccgc ctcgtcgacc acgtccacac
     2401 cgccaccgcc cgctgaccgc gcggggcgcc caccccgggg accgtgcccg cccgccggac
     2461 ggcgggccgc gcggccccgg cggcggggga gcaccgggcc gcagcggccg ggcggccgcc
     2521 gcgccggccg cgaagcccac ggcacgcacc accggcccga cgcgatccgg gcccgtggcc
     2581 gcagcggcgg cgggaggacc gggaacggca ccggcacccg gacgcgcgcc cccggacacg
     2641 gcaccggaca cagcaccggg cactcgcccg gtgcccccgc gaccgctgcg cgcgcgggcc
     2701 gacggtgtgc ccgagcccgc ggtcaccggc gcgcggccgt acgggaccgc acggccgcgg
     2761 acggcgcgaa gcgcacgcgc cccctccggt ccccgacggt ggtaccggcg atccggcagg
     2821 cgggcgtccc ttcagaccac gggcacgcgt gccgggcccg ggccggaggg tggacggcgg
     2881 gcggcgggcg gcgggccgga ggccccgccg cgccgaccgc ggggagcgcc ggcaggaccg
     2941 ggtccgccgc tccacccgcc ctccgggagg gtcgctcccg gtgtctcgcc ccgctccacg
     3001 gacgcggagg acgtacgtcg cgccgggggc gcaccaccgg cggccggttc acaagccagt
     3061 cgaccgcttt ttattgacaa gtcgttcgac cggtttctaa tctgcgtcac ggggcgggag
     3121 gaccgcgccc catccgtcgg gtgccgcacg gacggggacg ggccggggcc gacgggcagg
     3181 cagcgggcgc gtgggatacc tcctgccggc ccgatctcgg gggcgggggc caacgcatgg
     3241 cgaagcagga acgctcggaa cacacgcagg ggaagctgat cgtcgcggca gccgagcagt
     3301 tcgcgctccg cggcttcctc aaggcgacgc tcggcgacgt gagtcgggag gccggcgtga
     3361 cgaagggggc gctgttcttc cacttcgcca ccaaggacga cctcgcggca gccgtgcaga
     3421 ggcggggccg ggacatcctg gagacgatga tcgcggacat gtccggatcc gaggagtcct
     3481 gtctccagat cgtcgtggac gccctgcacg ccctgagccg gatgctgcgc tcggacccct
     3541 tcgtccgggc cggcgtgcgg atcgcgcggg agcggacggc tgagagcccg gtgcccgtgg
     3601 acttctatgc gctgtggctg ggaacgctgg gggaggtact cgaaagggcg cgcaagaacg
     3661 gcgagttggc cgccgatgtc gccgatgcgt ccgcacgcgc cctggtcacg acggtggtct
     3721 gcggcgtcga gacgctggcg tggatgggcg tgtccgagga cgagggcgac aagtggctcg
     3781 gttgcctgct ggaactggtc cttccgtcca tcgcgtccgc ggacggcctc cggcgcgtca
     3841 ggatcccggg gctccggggc acaccgtgag gcacgggcgc gtgccggcac ggtgaaccgc
     3901 gtccgcccgg acgaccgcgt ccgcccggac gcgtcgcgac ggacgtgaac gcggggaggc
     3961 ggcgcacggg gagacggcgg cggccaggcg tcccggcccg tcttcggccg gccgggacag
     4021 ccgtccgagg gggccggcgc cgtcgccctc ccggcgtccg ggtcgtccgg tgccgcccct
     4081 ggcccggggc acgccggacc ccgcggccgg ggcctcagcc cgtggccgcg ccgtcctcga
     4141 gcgcggccca cagctcctcg gaccccgcgg gcgcgaaccg ggcgaggcgg cgagccggga
     4201 cgaccccggg cagcagccac gtccacaggt ccgcgacccg ctcgtgcagg tcctcccggg
     4261 cggtccgcac ctgcgaggtg atctgcaccc ccgtgaacgc ccccacgatg taggtggaca
     4321 gatcgccgac gtccacctcg gacttgaggt caccggcgcc ctgggcgttc gccagcatgg
     4381 cgcggacgga gtcgatccac tggttgtagg gggagggatc gggtgtcgag aacgagccgc
     4441 gctctatgac gagccggatg ccggcccgca accgggcgct ggagcgcagg ccgtgggcca
     4501 tccggtgcgt caggtcgatg gcccgctgca gggcccgcag ctcggactcg gcgccgccgg
     4561 ggagcagttc gaactgttcc gccaccagtt cccgggccag cgcctccttc gactggaagt
     4621 ggaagtacat ggctcccttg gtgacgccgg cgtgctgggc gatgtcggtg aggctggtgc
     4681 tgccgaatcc cgccgcgtcg aacgcggtcg ccgcgccgtc caggatcgcc cggcgcgtgg
     4741 cctccgcccg ttcctgccgt gtcctgcttc ccgtcgccac agccgaccct ccgttccgtc
     4801 gtggtgcgga taacggcccc cccgtccgtc cggtgatcac cacgttctcc aacgcattcg
     4861 atcttcttca ctccgggcgg agaataccat tcgactagta ttctcggccg cgggcttccg
     4921 gatgctctca gaccctccgc atccataacg gcattgatca gagcgtgttc tcacatcagc
     4981 tgctatcgcc aggaggcaag atgaatgccc ttctgcaagc cgaccgaccg tatttcggaa
     5041 aaccggagga gtgcggtccc gagctgttcg aacagaccgt tccgcgcacc ctcgtccacc
     5101 gcgccgccgt ctccgaggtg ttgctgaccg ggtggcagac cgtccacgac gagcaccgcc
     5161 tcggagcgca gtggtcccgc gcgcacagct actacggatc ggtcgacggc cgctggcacg
     5221 acccgatgct gttcgccgag acgatccggc aggcctgcct gctgctggcc cacgaagccc
     5281 tcgacgtccc cctgggcttc ggcttcctca ccgagtccac cgcgttcgag gtcgacgagg
     5341 aacgcttacg cctctcccgg cccgcccacc ccgcccacgt ggtgctcgac atgcgcctca
     5401 gccacatcaa gcgccgggcc ggggtggtca gcagctacgc ctacgacgtc gtcgcccacc
     5461 gcgacggcga gcggctcggc agcggccggc tgagaggcaa gtgcacctcg caggcggtct
     5521 accggcggct gcgcggcgag cggatcggcg ccggtccgcc gcacaccgcg atcgccccgg
     5581 tcgacccccg gctggtgggc cgggacgacg agtccgacgt cgtgctgggg gcctccgccg
     5641 gtgccggcgc ccacccgctg cggatcgtcg ccgaccaccc ggtgctcttc gaccacccgg
     5701 tggaccacgt gcccggcatg gtgctcatgg aggcggcccg gcaggcgggc ctgctggcgc
     5761 tgggcgcgcc ccacggcctg ctggtcgcct gcgaggcgca gttcagacgg tacgtcgagt
     5821 tccacatacc ctgcctcgtg gccacggacg aaccggagca cgagggcggg ggacgccgca
     5881 cggtcaccgt ccgcttcctc caggacggca gcgaggccgg gagctgccgg gtcgggatcc
     5941 tcaccacggc cggggaggcc gacggagcgg ccaccgcaca gcactgaacg gggtcctgtc
     6001 ccggatccgc gccggtgccc cgtgacgcgg ccccgccgga acgccacggt tcgtggagga
     6061 ccgtccacga gcggcaccgc cgccggacgg ccggcgcccg cggggccgga ccgcggatcc
     6121 cgggaagggg aggacacccc ggcaccaccg ccccgatgag ggacccggac ggtcccgggg
     6181 cggtccgacc cgcgggatcc acctcgccgg tgagggcggc cagcgccctc accggcctcc
     6241 cggtcacggg gcgtcgtgga agatcacgcc gaggacgtgc cgttggccgt cgtgcaccgc
     6301 gctcaccccg tggcgcagca tgaaacggct ccagccccgc gccgacggga ccggacggtc
     6361 gtgcaccgcg aagatcagcc cctgcccctg ccccggccgc ttcaccaccg gacgggactg
     6421 ggcgcgcgga cgctgctcga cgaagacgct ctcaccaccg gtgaagtcga cgtcgggacg
     6481 gtccagcatc accgccacct gcagcgggaa cgtcagctcg ccgtagacgt cctggtgcag
     6541 gcagttgtag tcgcccgggc cgtagcgcag cagcaggggc gtgggccgca gctgtccggc
     6601 cgcggcgcac gccgccgtca gcccggcgtg cgtctgcggg aaccggggcc ggtccaggcg
     6661 ctccgcccac tggttggcga tccgggccag cggcgggtac agctgccggc gcagggcggc
     6721 caccaccggc ggcaggggcg cggcgaaata gcggtagcgg ccgctgccga agcggtggcg
     6781 gcccatgtcg acggtgctgc ggaacacctc gtcccggtcg aacagcgcgc ggacctcctc
     6841 gcaggcgcgc ggtgacagca ggggcggggt gacggcgacg ccctcggcgt ccagcgcggt
     6901 ggtcagcgag ggccagtcca gcgcgtccac ggcgggcagt tcgaccgcgt cgtcggcgcc
     6961 ggcgtcgggc gcggcggggg cacggccctc ttccatgccc ggcaggtggg ggatgtcgta
     7021 gctggtcagc gtgggctccg tggtcgtgga cgaggacggg cgccggaggc gggggattgc
     7081 tccggcgccc gccggttcag agggaggccg gggacgtgcg gccggactcc aggtcgagca
     7141 ggaaccgctt ggccgcgacc ccgccggcgt aaccggtcag gcggccgccc gagccgacca
     7201 cccggtggca gggcagcacc acgcacaggg ggttcgcgcc gagcgccgtg cccacggccc
     7261 gcgcggcccg cggccgcccg agcgcggcgg ccagctcggc gtaggtgccc gtcgccccgt
     7321 acgggaggag gccgtccagc cgctccacgg tcgtgcggct gaacggcgtg gccaggcgca
     7381 gatccatccg gacggtgaac gcgcggctgc ggccctcgag gtaggcgtcc agctgacggc
     7441 ccgcctcctc gaggatccgc cgctggtccg ccgtggcggc gtcggcgtcc acggcggtga
     7501 ggccgccgcg cccgagccgg gccagcgcct gctcgcggtc gtcgaaggcg cacagcacca
     7561 gcgcgtcgtc ggtggccgcg agggtcaggg gacccagcgt ggtgcggtgc ggggcgagtg
     7621 ccgcggtggg tggggtggac atggggacct ctctgtggtg ctccgtggtg cgccgggtca
     7681 gacggtgagg ggccggcagc tcttgcaggc ccggtaaccc gcctcgcggg cgtcccgcgc
     7741 ggtggggaag tgcacccggt gccccggggt gatgcggcgg gcgtgggcac aggtcgggtg
     7801 gcagtagatg aaggtggtgt cgctgccgag gaacaccgcg ccggcccggg acagctccgc
     7861 gacccggcgc atgtcgatgt gctcggcccg gcgcagggcc tcgcccgcct tcggcaggta
     7921 ggcggcgtcg cacggcgatc cgttgtcatg cgtgacccgg tggcacggta tgagcacggc
     7981 gacggggttg gccgtcagcg cgcccaggac gtcggagacc gggggcgggg acccggcgtc
     8041 cgcctcgcgg gcgatccagc tcagcggccg cagctgcccc ggggggatgc tgcgcacggc
     8101 ctccaggatc gcccgctgcc cctccggcag gccggacagg tcgatcgcca gccggcgggc
     8161 ccggccggtg cgcagcgcgg ggaggaggcc gggcagcggc ctgccccccg ggatcgccga
     8221 gcggccggta cgcgcgcggt gcagttcctc gaaacccgtc gcggtcaggc ccgccgagtc
     8281 caggcaggca ccggtcacgg tctgcgggct cgcggccacg aacagtccgc ccgcggccgt
     8341 gtcgaggcgc acgtacgtgt cgtagtgctc cctggagaga cccgcacggc gcagcacgcg
     8401 cagcgcgaag tcctccggcg caccggccgc gagatccggc agacgccggg tgaccgcgtc
     8461 gccgttgcct tcgctcacat cgtcacctcc ttcaacaccg tcggctccgg ttcgagcagc
     8521 cgccgcagac cggccagccc gcgggagacc tgcgccttgg cggtgccgac cgcgcagccc
     8581 tgcacctcgg ccacctctgt gtagctcatg ccgatcacat ggtggagcac gaccggtatg
     8641 cggtggtgct ccggtaaggc cgacagcgcc gtgaccaggc gcgaccggtc ctcggccgtc
     8701 gccgcgcgct cgtccgggcc ggggctgtgg tccggccaga cgtccatgct ctcctccacg
     8761 ggcagccccg cggtgaccgg acgccgggtc cgcgtgcgga cgtgattgcg ccacacgttc
     8821 gccgcgatgg tcatcagcca cgcacgggga cgcagctcac ggcgccgtgc gggagcgtac
     8881 tcccgcaggg ccgtgtaggc gcgcagaaac gtgtcctggc ccaggtcgtc cgcctcggcg
     8941 gcggacccgg agacccggta caggaacgcg tgcaccgccc cggcgtgcac ccggaccagc
     9001 tcggtgaagc cggcttcgag gtgcaccgcc aggagatcgg tcagctgctc ggccttgtcg
     9061 tccatcactg aaacaaacac cgtcccgggc cagggggttg cagacggggg gaaacggtca
     9121 tccgacgacc ggggcgtcca gggccgagct gccgaccagg atcgcctggt acaggctcct
     9181 catccggggg acgggctcga tccccaggga gtcaccgagc gttcggcgca gcccgtcgaa
     9241 gacgtcaccg gcgcgtttgc gctcgccgga gcggtacagc gccaccatca gctgagcgtg
     9301 cacgttctcg tgggtggggt ggcgcgcggt caggtcgctc agctcgtcga ccagctccct
     9361 gtgcttgccg agggccaggt cggcctctat gcgctgctcc aggaccccct gccgggtctc
     9421 gttcagctcc gccaggtgcg gcgagctgag ggggcccgtg cggacgtcgg acagcgcggg
     9481 cccgcgccac tgcgcgaggg ccagggtgaa catctccgac gccgtgcggt ggtccccggc
     9541 ggcggccgcc acgcgtcccc gccgggtcag atccgtgaac cggaagcggt cgaagtcggt
     9601 cgtctcgagg gcgagctggt aaccctggtt ccgggtgacg agcagatgac tgaggtcacc
     9661 gcgggggccg cgcagggcct ggcgtatgtg gagcacgtag gtctggagcg tggacatcgc
     9721 gctcttgggg ggagtcgacc cccacagctc ggtgatgcag tcgccggccc gcaccatttg
     9781 cggcgtgctc agcatcagga atgcgaggag ctggcgcggt ttcggtgcgc tcggtaccag
     9841 attcacgccg gaatcggaca gtaagagggg gccgagaacg cccgagttca taaagaattc
     9901 ctatccccgt agtgtcgtag cacctcgaac aggcatccta ttttttctcg aatcgaagat
     9961 aagcgggcaa cgtttccgtg tcaatgttcc ggtttcgggt ggaagggtgc ccgataatga
    10021 attcggtcgg ctggccggtt cttttttctt gatattcggg aaggcggcgc ggcggccgtc
    10081 cggccacgac gaaacggccc cggaccgtga cggtccgggg ccgtgttcgg gtggaggcgg
    10141 gttaccggcc ggcggcccgg cgcagcgcgt cgacgcggtc ggtgcgctcc caggtgaagt
    10201 cgggcagctc ccggccgaag tggccgtagg cggcggtccg ggcgtagatc gggcgcagca
    10261 ggtccagatc gcggacgatc gcggccgggc ggaggtcgaa gacctccgtc acggccttct
    10321 cgacggcggc ctgcgggacg gtggcggtac cgaaggtctc cacgaacagg cccacgggct
    10381 ccgccttgcc gatcgcgtac gccacctgca cctcgcagcg ggcggccagg ccggccgcga
    10441 ccacgttctt ggccacccag cgcatcgcgt acgccgccga acggtccacc ttggaggggt
    10501 ccttgccgga gaaggcgccg ccgccgtggc gggcgtaacc gccgtaggtg tcgatgatga
    10561 tcttgcggcc ggtcaggccg gcgtcgccca tcgggccgcc gatctcgaac cggccggtgg
    10621 ggttgaccag caggcggtag ccctcggtct ccagcttgac gccctcctgg gccagttcgt
    10681 ccaggacgtg ctcgacgacg tgctcgcgca ggtcgggggt gagcagcgag tccaggtcga
    10741 tgccgctcgc gtgctgggag gagacgacga cggtgtccag gcgtttgggc ttgctgccca
    10801 ggtactcgac ggtgacctgg gtcttgccgt ccggacgcag gtagggcacc gtcccgtcgc
    10861 gccgcgcctc ggacagccga cgcgacaggc ggtgggccag atggatgggc aacggcatca
    10921 ggctgggcgt ctcgtcgcag gcgtaaccga acatcaggcc ctggtcgccg gcgccctgga
    10981 gggccag
//
