LOCUS       NZ_FMZK01000001        10399 bp    DNA     linear   CON 26-APR-2024
DEFINITION  Streptomyces prasinopilosus strain CGMCC 4.3504, whole genome
            shotgun sequence.
ACCESSION   NZ_FMZK01000001
VERSION     NZ_FMZK01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces prasinopilosus
  ORGANISM  Streptomyces prasinopilosus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900101585.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 02:19:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,269
            CDSs (total)                      :: 6,190
            Genes (coding)                    :: 5,988
            CDSs (with protein)               :: 5,988
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 202
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 202
            Pseudo Genes (frameshifted)       :: 63 of 202
            Pseudo Genes (incomplete)         :: 175 of 202
            Pseudo Genes (internal stop)      :: 8 of 202
            Pseudo Genes (multiple problems)  :: 40 of 202
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:44:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1255434
            Orig. end                         :: 1265833
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FMZK01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    38..53
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.76"
                     /tool="antismash"
     gene            781..1389
                     /locus_tag="BLS36_RS05545"
                     /old_locus_tag="SAMN05216505_1011106"
     CDS             781..1389
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="BLS36_RS05545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_1011106"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_074993660.1"
                     /transl_table=11
                     /translation="MDVNLPQLPFPLRTYGPDGNWSHEDGVLTGWAGPRQDRFVPPTGE
                     ALDPATDAPRLLGAPEGDFQLIARVTVGFSAAFDAGVLYVHTGERNWAKLCLEYSPDVP
                     TVCTVVTRGHSDDANSFTVEGSSVWLRVSRTGRAFAFHASRDGEHWTFVRLFTLDAEKE
                     TGAALVGFMTQSPMGEGCVVTYDHIEFRPEWPRDLRDGS"
     gene            1485..1991
                     /locus_tag="BLS36_RS05550"
                     /old_locus_tag="SAMN05216505_1011107"
     CDS             1485..1991
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019060614.1"
                     /locus_tag="BLS36_RS05550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_1011107"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_176954888.1"
                     /transl_table=11
                     /translation="MTTTNRPAASPVIRPALAAETADLAALHLRARSTYYPDGIPEDGT
                     DWPAVWREAVTRPDGRVLCAVRDGRLAGLASFRTPPDAPAGTVKLFQFHVDPGHWRTGT
                     GTALHAACVARWRADGLRTAVLDVHTGNHRARAFYARLGWTPDPAHPPAPGDHHLSLRF
                     AVPGE"
     gene            2066..2776
                     /locus_tag="BLS36_RS05555"
                     /old_locus_tag="SAMN05216505_1011108"
     CDS             2066..2776
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545807.1"
                     /locus_tag="BLS36_RS05555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_1011108"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_074993791.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALGAFPHRDEVEVVHRSFELDP
                     GRAKDDVQPVLTMLTEKYGMSRAQAEAGEDELGAQAAAEGLPYRTRGRDHGGTLDMHRL
                     LHFARGEGRQEELLDLLYRANFAEERSVFGDDDRLLELAGAAGLDTEAARAVLADPDAY
                     AAEVREDQREAAELGARGVPFFVLDRAYGVSGAQPAEVFTRALTQAWDARPRLETVEGG
                     AEVCGPDGCAVPQT"
     gene            2917..3978
                     /locus_tag="BLS36_RS05560"
                     /old_locus_tag="SAMN05216505_1011109"
     CDS             2917..3978
                     /NRPS_PKS="Domain: Aminotran_5 (21-347). E-value: 1.6e-24.
                     Score: 78.5. Matches aSDomain:
                     nrpspksdomains_BLS36_RS05560_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671936.1"
                     /locus_tag="BLS36_RS05560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_1011109"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_055574367.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.5e-19, bitscore:
                     65.1, seeds: 34, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 5.7e-06,
                     bitscore: 21.9, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTLDTLERLVRTEFTPGNTFLNTAANALLPARAVTALEGAARLR
                     AAGGPLDPLYDDVEAGRAAYARLAGVPAGRVAVGATAALYTALVAASLRPGAEVLTAEN
                     DFASVVNAFHVRGDLEVRTVPLERLAGSVGPDTALVAVSAVQSADGRIADLDALREAAR
                     AHGARTYVDLSQAIGWLPVDAGAFDYSVAIVYKWLLGPHGGAFLTVPEDFGDLLPVQAA
                     WAAAENPWNSAYGPVAELAHSARRFDTSPALFSYTGLRASLELLEEIGVDAVHAHDVAL
                     ADRFRAGLAALGHEPVPSPGSAIVSVPGLGRLQPELSRAGFALSDRAGHLRASFHLYNT
                     PADVDRLLDALPG"
     misc_feature    complement(2936..2950)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: strong, score: 21.84"
                     /tool="antismash"
     aSDomain        2980..3957
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLS36_RS05560_Aminotran_5.1"
                     /evalue="1.60E-24"
                     /label="BLS36_RS05560_Aminotran_5.1"
                     /locus_tag="BLS36_RS05560"
                     /protein_end="347"
                     /protein_start="21"
                     /score="78.5"
                     /tool="antismash"
                     /translation="LNTAANALLPARAVTALEGAARLRAAGGPLDPLYDDVEAGRAAYA
                     RLAGVPAGRVAVGATAALYTALVAASLRPGAEVLTAENDFASVVNAFHVRGDLEVRTVP
                     LERLAGSVGPDTALVAVSAVQSADGRIADLDALREAARAHGARTYVDLSQAIGWLPVDA
                     GAFDYSVAIVYKWLLGPHGGAFLTVPEDFGDLLPVQAAWAAAENPWNSAYGPVAELAHS
                     ARRFDTSPALFSYTGLRASLELLEEIGVDAVHAHDVALADRFRAGLAALGHEPVPSPGS
                     AIVSVPGLGRLQPELSRAGFALSDRAGHLRASFHLYNTPADVDRL"
     gene            complement(4083..4994)
                     /gene="thpD"
                     /locus_tag="BLS36_RS05565"
                     /old_locus_tag="SAMN05216505_1011110"
     CDS             complement(4083..4994)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976953.1"
                     /locus_tag="BLS36_RS05565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_1011110"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_055574366.1"
                     /sec_met_domain="PhyH (E-value: 4.3e-40, bitscore: 134.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVTPGTQTHVPDLYPTRGNAEVLTPRQDPVVWGAPDAPGPISP
                     ADLGSYERDGFLAVDQLITDDEVAVYRDELNRLVADPAVRADDRSIVEPKSKEIRSVFE
                     VHRISEVFARLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAE
                     DGLPRMRAVSVSIALTENLDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTP
                     SDEGLTSLASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAV
                     EPFAAPVRRPEYIGARDFTPVR"
     gene            complement(5001..5399)
                     /locus_tag="BLS36_RS05570"
                     /old_locus_tag="SAMN05216505_1011111"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="BLS36_RS05570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_1011111"
                     /product="ectoine synthase"
                     /protein_id="WP_055574365.1"
                     /sec_met_domain="ectoine_synt (E-value: 7.4e-54, bitscore:
                     177.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEIEGTDRHVKSASGTWESKRIVLARENVGFSLHETILY
                     AGTETSMWYANHIEAVVCVEGEAELTDHDSGLTHTITPGTMYLLDGHEKHTLRVKKDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEEV"
     gene            complement(5568..6839)
                     /gene="ectB"
                     /locus_tag="BLS36_RS05575"
                     /old_locus_tag="SAMN05216505_1011112"
     CDS             complement(5568..6839)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 5.6e-74.
                     Score: 241.1. Matches aSDomain:
                     nrpspksdomains_BLS36_RS05575_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="BLS36_RS05575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_1011112"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_055574364.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7.7e-79, bitscore:
                     261.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAHGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLESFQNWILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGAVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALAELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTATAALEAYWADGSAMEKQTRARGEQVEQAL
                     AAVAEENRAAVKEYRGRGLVWGMEFHDKERAGRIAARAFELGLLIETSGPEGEVVKLLP
                     ALTITPDELDEGLTTLARAVRETA"
     aSDomain        complement(5742..6755)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLS36_RS05575_Aminotran_3.1"
                     /evalue="5.60E-74"
                     /label="BLS36_RS05575_Aminotran_3.1"
                     /locus_tag="BLS36_RS05575"
                     /protein_end="366"
                     /protein_start="28"
                     /score="241.1"
                     /tool="antismash"
                     /translation="FDRAHGSRMYDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLESFQNWILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGAVEDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALAELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TATAALEAYWADGSAMEKQTRARGEQVEQALAAVAEENRAAVKEYRGRGLVWGMEFH"
     gene            complement(6931..7437)
                     /gene="ectA"
                     /locus_tag="BLS36_RS05580"
                     /old_locus_tag="SAMN05216505_1011113"
     CDS             complement(6931..7437)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140785.1"
                     /locus_tag="BLS36_RS05580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_1011113"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_055574363.1"
                     /transl_table=11
                     /translation="MTAAQADLHVDRPTPADGAALWRIARDSKVLDLNSSYSYLLWCRD
                     FAATSAVAREAGGEPVGFVTGYVRPDSPHTLLVWQVAVDETHRGRGLAAALLDGLAART
                     VAEHGVTTVETTITPGNTASERLFTAFAERHGARLEREVLFEAEQFPDGPHDPEVLYRI
                     GPLTP"
     misc_feature    7447..7462
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.99"
                     /tool="antismash"
     misc_feature    complement(7462..7474)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7593..7608)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            complement(8210..9313)
                     /locus_tag="BLS36_RS05585"
                     /old_locus_tag="SAMN05216505_1011114"
     CDS             complement(8210..9313)
                     /NRPS_PKS="Domain: Aminotran_5 (75-341). E-value: 3.6e-28.
                     Score: 90.4. Matches aSDomain:
                     nrpspksdomains_BLS36_RS05585_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="BLS36_RS05585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_1011114"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_074993662.1"
                     /sec_met_domain="Aminotran_5 (E-value: 8.2e-23, bitscore:
                     77.0, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTHPFLGLAPLSAARFASIEDRVARLLRTAQDVVIMQGEALLPL
                     EGAIRATAGPGTTALNVVTGPYGQTFGDWLRDCGATVIDLAVPFHTAATAGRIREAFAA
                     HPEIDFVSLVHAEAATGNTNPVAEIGEAVRAHGALFYLDAVASIGAEPVLPDAWGVDLC
                     VIGAQKAMGGPAGVSAVSVSERAWARMAANPRAPRRSYLSLLDWKQRWIDGGRTALPHA
                     PAQLEMLALEACVERIEAVGLETVTARHAAAAAATRAGTLALGGGLEPYVHDAAQAAPV
                     ATTLRVPSGTVASELVGRALAVDPALPLAAGGGPLAKEMVRVNHYGPDATRGVVRASLA
                     ALGTALAEQGLPVDVQGALRAADHGWR"
     aSDomain        complement(8291..9088)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLS36_RS05585_Aminotran_5.1"
                     /evalue="3.60E-28"
                     /label="BLS36_RS05585_Aminotran_5.1"
                     /locus_tag="BLS36_RS05585"
                     /protein_end="341"
                     /protein_start="75"
                     /score="90.4"
                     /tool="antismash"
                     /translation="RDCGATVIDLAVPFHTAATAGRIREAFAAHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEAVRAHGALFYLDAVASIGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWARMAANPRAPRRSYLSLLDWKQRWIDGGRTALPHAPAQLEMLALEACVERI
                     EAVGLETVTARHAAAAAATRAGTLALGGGLEPYVHDAAQAAPVATTLRVPSGTVASELV
                     GRALAVDPALPLAAGGGPLAKEMVRVNHYGPDATRGVVRASLAA"
     misc_feature    complement(9497..9510)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.15"
                     /tool="antismash"
     gene            9538..10392
                     /locus_tag="BLS36_RS05590"
                     /old_locus_tag="SAMN05216505_1011115"
     CDS             9538..10392
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="BLS36_RS05590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_1011115"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_055573439.1"
                     /transl_table=11
                     /translation="MKTLLGRRTRVLAATTATAGLVLLTACTSTDEGDGGASKTAAGGV
                     ELAKAGQLTTCTHLPYPPFQSEIDGKVQGFDVALIDLVAEDLDVKQAILDTPFENFKTG
                     AFLNSGECDLAAAGMTITEERKKNVDFSDPYFNATQAVLVDKKAGVTSLADVKAKNVKL
                     GAQAQTTGEDYVKKEGFDPVSFESSDAVLNGLRTGQVKAVVIDFPVVQGWLKDKATADA
                     YEVVDNLDTGEQYGFTVKKGNTALRKAVNKALSDAKADGTYKKLYEQWIGPYDESAAEP
                     SAT"
ORIGIN
        1 aagccggggc tcgtcctcgc ccccgcgggc gcgggcggcg accgcctggt caagtccctg
       61 accgacgccg tcagcgacac cgacgccggc gtcctgctcg accaccgcat gcgcgaccac
      121 ttcgtcgccg gggtcgccga gcgggcccaa ctgcccgacg tggagtcccc ggtgacgccg
      181 ggcgcgggcg gcgagcacac cgtcagcgcc ggcttcctca ccgtcccggc gagcaggctc
      241 gccgcggagg gcgagcacga cctgctcctg gaggagtgct tcggcccggt caccgtcgtg
      301 gtccgctacg aggacgacgc cgaggtccgc accgtcctct cccgcctccc cggcaacctc
      361 accgccaccg tccacctctc cgaggaggag gccgcgggag agggcagggg agcggagatc
      421 ctcggcgaac tgaccccgct cgccggccgg gtgctcgtca acgcctggcc gaccggtgtc
      481 gcggtcgccc ccgcccagca ccacggcggc ccctacccgg cgacgacctc cacctccacc
      541 tcggtcggcg gcaccgccat cgagcgctgg ctgcgccccg tcgcctacca gaacagcccg
      601 caggccctcc tgccgcagga actgcgcgac gacaacccgc tcgggctgcc ccgccggttc
      661 aacggcgtcc tggagcgctg accgcgcacc gcggagcggg tccacccgcg tgcacgggcc
      721 cgtccggcgt gatccgccgg gcgggccccg cgcgcgcgga cgacctgcga aactcctccc
      781 atggacgtga accttcccca actgcccttt ccgctgcgca cctacggtcc cgacgggaac
      841 tggtcccacg aggacggcgt cctcaccggc tgggccggac cgcgccagga ccgcttcgta
      901 ccgcccaccg gggaggccct ggaccccgcc accgacgcgc cccggctgct gggcgccccc
      961 gagggcgact tccagctgat agcccgggtc acggtcggct tctccgccgc cttcgacgcc
     1021 ggggtcctct acgtccacac cggcgagcgg aactgggcca aactgtgcct ggagtactcc
     1081 ccggacgtgc ccaccgtctg cacggtcgtc acccggggcc attccgacga cgccaactcc
     1141 ttcacggtgg aaggcagttc cgtatggctg agggtcagcc gcacgggccg cgccttcgcc
     1201 ttccacgcct cccgcgacgg cgagcactgg accttcgtcc gcctcttcac cctcgacgcg
     1261 gagaaggaga ccggagccgc cctcgtcggc ttcatgaccc agtcaccgat gggcgagggc
     1321 tgcgtggtca cctacgacca catcgagttc cggccggagt ggccgaggga cctgcgcgac
     1381 ggcagctgag ggcgggggcg gcggggcggg ggaacccccg ggggccgggc ggaaagcgcc
     1441 accaccgggg aaccgccgtc ccggccgccc acgtcctgag ccgcatgacc acgacgaacc
     1501 ggcccgcagc ctccccggtg atcaggcccg ccctcgccgc cgagaccgcc gacctcgccg
     1561 cgctgcacct gcgggcccgc tccacgtact acccggacgg gatccccgag gacggcactg
     1621 actggccggc cgtctggcgg gaggccgtca cgcgccccga cggacgggtg ctgtgcgcgg
     1681 tgcgggacgg acgcctggcc ggcctcgcct ccttccgcac cccgccggac gcccccgccg
     1741 gcacggtcaa gctgttccag ttccacgtgg accccggcca ctggcgcacc gggaccggca
     1801 cggccctgca cgccgcctgc gtcgcgcggt ggcgggcgga cggcctgcgc accgcagtcc
     1861 tcgacgtgca caccggcaac caccgggccc gggccttcta cgcccgcctg ggctggaccc
     1921 ccgacccggc acacccgccc gcccccggcg accaccacct gtcgctgcgg ttcgcggtgc
     1981 ccggggaatg aaccgcgtcg gccggaggtt cacgcggacg gcggaggagg tctccgtgcc
     2041 ctgcgaccgg gagagtgccc caacgatgcg tgtcgagatc tggtcggaca tcgcctgccc
     2101 ctggtgctac gtgggcaagg cccgcttcga gaaggcgctc ggcgcgttcc cgcaccggga
     2161 cgaggtcgag gtggtgcacc gctccttcga gctggacccc ggccgggcca aggacgacgt
     2221 ccagcccgtc ctcacgatgc tcaccgagaa gtacggcatg agccgggcgc aggccgaggc
     2281 cggcgaggac gaactgggcg cccaggccgc cgccgagggg ctgccgtacc ggacccgggg
     2341 ccgcgaccac ggcggcaccc tcgacatgca ccgcctgctg cacttcgccc gcggggaggg
     2401 ccgccaggag gaactgctcg acctgctcta ccgggcgaac ttcgccgagg agcggtccgt
     2461 cttcggtgac gacgaccgcc tcctcgaact cgccggcgcc gccggcctcg acaccgaggc
     2521 cgcccgcgcg gtcctcgccg acccggacgc gtacgccgcc gaggtgcgcg aggaccagcg
     2581 ggaggccgcg gaactcggcg cgcggggcgt gcccttcttc gtcctcgacc gcgcgtacgg
     2641 cgtctccggc gcccagcccg ccgaggtgtt cacccgggcg ctgacccagg cctgggacgc
     2701 gcgtccgcgg ctggagaccg tcgagggcgg ggcggaggtc tgcggccccg acggctgcgc
     2761 ggtgccgcag acctgaaccg gccgttccgg aaccggcggc ccgtacccgc ggcaccgctc
     2821 cttcgcaggt cggggtgtat ccgtaaggat tcctcagcgg ttccgcgcag aacaagccga
     2881 tggacgggga agatcccggg gccgcagagt ggtgccatga agaccttgga caccttggaa
     2941 cgcctcgtcc gtaccgagtt caccccgggg aacaccttcc tgaacaccgc cgccaacgcg
     3001 ctcctgcccg cgcgtgcggt gaccgccctc gagggcgccg cgcggctgag ggccgcgggc
     3061 ggtccgctgg accccctgta cgacgacgtc gaggccggcc gggccgccta cgcccggctg
     3121 gccggcgtcc ccgccgggcg ggtggcggtc ggggccaccg ccgccctgta caccgcgctg
     3181 gtcgcggcct ccctgcggcc gggagccgag gtgctcacgg ccgagaacga cttcgcctcg
     3241 gtggtcaacg cgttccacgt ccgcggcgac ctcgaggtgc ggaccgtgcc gctggagcgg
     3301 ctcgccgggt ccgtcggccc ggacaccgcc ctcgtcgccg tcagcgccgt gcagtccgcc
     3361 gacggccgga tcgccgatct ggacgcgctg cgcgaggcgg cccgggcgca cggcgcccgc
     3421 acctacgtcg acctctccca ggcgatcggc tggctgccgg tcgacgccgg tgccttcgac
     3481 tactccgtgg ccatcgtcta caagtggctg ctgggcccgc acggcggggc cttcctgacc
     3541 gtccccgagg acttcggcga cctgctcccc gtccaggcgg cctgggccgc ggcggagaac
     3601 ccctggaaca gcgcctacgg gcccgtggcc gaactcgccc actcggcccg ccggttcgac
     3661 acgagccccg ccctcttcag ctacacggga ctgcgggcct cccttgaact cctcgaggag
     3721 atcggggtgg acgccgtcca cgcccacgac gtcgccctcg ccgaccgctt ccgcgcggga
     3781 ctcgccgcgc tgggccacga acccgttccc tcgcccggtt cggccatcgt gtccgtgccg
     3841 ggactgggcc gcctccagcc ggagttgagc agggcgggct tcgccctgtc cgaccgggcc
     3901 ggccacctgc gggcgtcctt ccacctctac aacaccccgg ccgacgtgga ccggctgctg
     3961 gacgcgctgc ccggctgaac cgcgcccgcc cgggcgggaa cggcggcggg ccggtgcctc
     4021 ccgtaccgca cgggaggcac cggcccgcgg tcacggccgc cgacgcgttc gggccggcgt
     4081 ggtcacctca ccggggtgaa gtcccgggcc ccgatgtact cgggccgccg gaccggcgcc
     4141 gcgaacggct ccacggccgt gttctcgacg ctgttgaaca cgatgaacac gttgctgcgc
     4201 gggaacggcg tgatgttgtc cccggagccg tgcatgcagt tgcagtcgaa ccaggtcgcc
     4261 gaaccggcct tgccggtgaa cagcttgatg ccgtactcgg acgccagcga ggtcagcccc
     4321 tcgtcggagg gcgtccccgc gtcctgcatc tgcagcgact tcttgtagtt gtccttcggc
     4381 gtggcccccg cacacccgag gaacgtcttg tgcgaccccg gcatgatcat gagcccgccg
     4441 ttggtgtcga ggttctcggt gagcgcgatc gacaccgaca ccgcccgcat ccgcggcagt
     4501 ccgtcctcgg cgtgccaggt ctcgaagtcc gagtgccagt agaagccgct ggccccgaag
     4561 cccggcttga cgttgatccg cgactggtgg acgtacacgt cggagccgag gatctgccgg
     4621 gcccggccga cgacgcgctc gtcgcgcacc agccgcgcga acacctcgct gatccggtgg
     4681 acctcgaaga ccgaccggat ctccttggac ttcggctcga cgatcgaccg gtcgtcggcc
     4741 cggaccgccg ggtcggcgac caggcggttc agctcgtccc ggtagacggc cacctcgtcg
     4801 tcggtgatca gctggtcgac ggcgaggaag ccgtcacgct cgtacgaccc caggtcggcc
     4861 ggggagatcg ggccgggggc gtcgggggcg ccccagacga ccgggtcctg tcggggcgtg
     4921 agcacctcgg cgttgccgcg ggtgggatag aggtcgggga cgtgggtctg ggtgccgggg
     4981 gtaacggtgg tcatggaacc tcacacctcc tcggtgagca gcgggtacac gccgttctcg
     5041 tcgtggtcct cccgtccggt cacgggcggg ttgaagacgc agaggcagcg gaagtccttc
     5101 ttgacccgca gcgtgtgctt ctcgtgcccg tcgaggaggt acatcgtccc gggcgtgatc
     5161 gtgtgcgtca gcccggagtc gtggtcggtg agttctgcct ccccctccac gcagacgacg
     5221 gcctcgatgt ggttcgcgta ccacatcgac gtctccgtac cggcgtacag gatggtctcg
     5281 tgcagggaga agccgacgtt ctccctggcg aggacgatgc gcttgctctc ccaggtaccg
     5341 gacgcggact tcacgtgccg gtcggtaccc tcgatctcct tgaaggaacg gacaatcacg
     5401 gtgctgcgat gcctcctcga tgggggtgcg gaaggtgccc gccggccgtc cgggggccgg
     5461 gccggggccc gggacccgga gggtgcggcg gacggttcag ggggtcaccc tggccgggtg
     5521 gcccggacgg accggggtga tccggcggtc cgggcggccc ggacggttca ggcggtctcg
     5581 cggacggcgc gggccagcgt ggtcagcccc tcgtccagct cgtccggggt gatggtcagc
     5641 gccgggagga gtttgacgac ctcgccctcc gggccggacg tctcgatcag caggccgagc
     5701 tcgaaggcgc gcgcggcgat gcggccggcc cgctccttgt cgtggaactc catgccccac
     5761 acgagcccgc gaccgcggta ctccttcacg gcggcgcggt tctcctcggc gacggcggcc
     5821 aacgcctgct cgacctgctc gccgcgcgca cgggtctgct tctccatcgc cgacccgtcg
     5881 gcccagtagg cctcgagggc ggcggtcgcc gtgacgaagg cggggttgtt gccgcggaag
     5941 gtgccgttgt gctcgcccgg ctcccagacg tccagctcgg gcttgaacag gcacagggac
     6001 atcggcagcc cgtagccgct gatcgacttg gacacggtga cgatgtcggg cgtgatgccg
     6061 gcctcctcga aggagaagaa ggcgccggta cggccgcagc ccatctggat gtcgtcgacg
     6121 atgaggagca tgtcctggcg ttcgcacagc tccgccaggg cgcgcagcca ctcgggccgg
     6181 gcgacgttga tgccgccctc gccctgaacg gtctcgacga tcaccgcggc gggcttgttg
     6241 aggccggagc cctggtcctc caggagccgc tcgaaccaca ggaagtcctc gaccgcgccg
     6301 tcgaagtagt tgtcgaacgg catcggcgtg ccgtgcacca gcgggatgcc cgccccggcg
     6361 cgtttgaagg cgttgccggt cacggcgagc gagcccagcg acatgccgtg gaaggcgttg
     6421 gtgaacgaca cgatcgactc gcgtcccttc accttccgcg ccagcttcag cgccgactcg
     6481 acggcgttgg tgcccgtcgg gccggggaac atcaccttgt acggcaggtc gcgcgggcgc
     6541 aggatccagt tctggaagga ctccaggaac gcgcgcttgg ccgtcgtcga catgtcgagc
     6601 ccgtgcgtga cgccgtcccg ctccaggtag tcgatcagcg cccgtttcag cacggggttg
     6661 ttgtggccgt agttcagtga gccggctccg gcgaagaagt cgaggtactc gtggccgtcc
     6721 tcgtcgtaca tgcggctgcc gtgtgctcgg tcgaacacgg tgggccagcc gcggcagtag
     6781 ctgcgcacct cggactcgag ggtctcgaag acgctcagat cgggctgggt gatggtcacg
     6841 acgaatcgct cctcagtggc gtgggaagtc agagggagta gtccggggga ccggcggagt
     6901 gacgcacggg tcgtgcgcgg gggccggtgg tcagggggtg aggggcccga tgcggtacag
     6961 cacctcgggg tcgtgcggcc cgtcggggaa ctgctccgcc tcgaacagca cctcgcgctc
     7021 cagccgggct ccgtggcgct cggcgaacgc ggtgaacagg cgctcggacg cggtgttccc
     7081 cggtgtgatg gtggtctcca cggtggtcac tccgtgttcc gcgacggttc gtgcggccag
     7141 accgtcgagc agagcggcgg ccaggccccg gccgcggtgc gtctcgtcca ccgcgacctg
     7201 ccagacgagc agggtgtgcg ggctgtccgg ccgtacgtag ccggtgacga agccgacggg
     7261 ctccccgccg gcctcccgtg cgacggcgga tgtggcggcg aagtcccggc accacaggag
     7321 atagctgtac gacgagttca ggtcgaggac tttcgagtcc ctggctatcc gccacagtgc
     7381 ggctccgtcc gccggggtcg ggcggtcgac gtgcaggtct gcttgtgcgg cagtcatgtg
     7441 aattgaactt acccaggtaa attcgaaatt gcatcggctg tcggggttac ctgagtggct
     7501 tgtctatgtt atcgcgcgag cgtgaggccg caagcggaga ggtggtggaa tgccccggtt
     7561 cgtgcgtgat ttaaggggag aatcgggcgg cttgtggatc tgatcacaag tccataactc
     7621 ccgcgacggt gtccgggatg cgatggtttc cagctgcgga aaattggctg gtttaaggtt
     7681 ggcgaatggg ggcagaagat gaagggaagc tgccctggaa ggtaataggg aaataagagg
     7741 gagaagggcg ggggaatacc cgggaatggc tttcgataat cccgtgaatt cacgctcggg
     7801 attccgcttt cccgtgggtc gcgccttcgc cgcgccgcgg tgccgccgtc gacgccctcc
     7861 gggaagggac cggaacggga cgtcacggct cgcacacgct ccggccgggt gggcgctcgc
     7921 gaacggcccg gacccggccc gcgtccgtga gcggtccgac agcggtcccg cgcggacgac
     7981 cccgcggggg tccggtgcgc acacggtccg gcgaggttcc gacgcgcaca cggcccggcg
     8041 gggttccgac gcgcggacgg tccggcagcg gtccgcccac cgccgtccgg ccccgccccg
     8101 cgggcggttc ccgctcacgc cccgccccac ggtgtcccgc ccggcctttc acccccggtg
     8161 ccgggagggc gtgcccggcg ccgggggtgc cgcgccgggc ggtggccggt taccgccagc
     8221 cgtggtcggc cgctcgcagg gccccctgca cgtccaccgg caggccctgc tccgccagcg
     8281 ccgtgcccag ggccgccagg ctcgcccgga ccacgccccg ggtcgcgtcg gggccgtagt
     8341 ggttgacccg gaccatctcc ttcgccagcg gcccgccccc cgccgccagc ggcagcgccg
     8401 ggtccacggc cagggcgcgg cccaccagtt cggaggccac cgtgcccgac gggacccgca
     8461 gggtcgtggc caccggggcg gcctgggccg cgtcgtggac gtacggctcc aggccgccgc
     8521 ccagcgcgag cgtccccgcc cgggtcgccg ccgcggccgc cgcgtgccgc gccgtcaccg
     8581 tctccaggcc caccgcctcg atgcgctcca cgcaggcttc cagcgccagc atctccagct
     8641 gcgccggagc gtgcggcagt gccgtgcggc ccccgtcgat ccagcgctgc ttccagtcca
     8701 gcagggacag gtacgagcgg cgcggcgccc gcgggttcgc cgccatccgg gcccacgccc
     8761 gctcgctcac cgacaccgcc gacacaccgg ccggaccgcc catcgccttc tgcgccccga
     8821 tcacgcacag gtccacgccc cacgcgtccg gcagcaccgg ctccgccccg atcgacgcca
     8881 ccgcgtccag gtagaacagc gccccgtggg cccgcaccgc ctcgccgatc tccgcgaccg
     8941 ggttggtgtt gccggtggcc gcctccgcgt gcaccaggga gacgaagtcg atctccgggt
     9001 gcgcggcgaa ggcctcgcgg atccgcccgg ccgtcgccgc cgtgtggaac ggcaccgcca
     9061 gatcgatcac cgtggcgccg cagtcccgca gccagtcgcc gaaggtctgc ccgtaggggc
     9121 ccgtgacgac gttcagcgcg gtcgtgccgg ggccggcggt cgcgcggatc gcgccctcca
     9181 gcggcagcag cgcctcgccc tgcatgatca cgacgtcctg cgcggtgcgc agcaggcgcg
     9241 ccacgcggtc ctcgatcgag gcgaagcgcg cggcgctcag cggggccagg ccgaggaagg
     9301 ggtgggtgct cacgacgttg ctctccagat cacggcttct acggcgccac ggctcgcgca
     9361 gggaccacgg ggcacggggc acgggttcga cggagtcgag cgtatccccg cccccggtga
     9421 ccccggcccg acgcccggtt cttaggccgg acgaccccgc agccatcggt ttggtttgag
     9481 gaacccaaac ctctccttat aatcagaacc tcatccgttc cctaccagga ggcctcagtg
     9541 aagacgctcc tcggccgcag gacccgtgtc ctggccgcca ccaccgcgac ggccggactg
     9601 gtgctcctga ccgcctgcac ctccaccgac gagggggacg gcggcgcctc caagaccgcg
     9661 gccggcggcg tcgagctcgc caaggcgggg cagctcacca cgtgcaccca cctcccgtac
     9721 ccgcccttcc agtcggagat cgacggcaag gtgcagggct tcgacgtggc cctgatcgac
     9781 ctggtcgccg aggacctgga cgtgaagcag gcgatcctcg acacgccgtt cgagaacttc
     9841 aagaccggcg ccttcctcaa ctccggcgag tgcgacctgg ccgcggccgg catgaccatc
     9901 accgaggagc ggaagaagaa cgtcgacttc tcggacccgt acttcaacgc cacccaggcc
     9961 gtcctcgtcg acaagaaggc cggcgtcacc tcgctcgccg acgtgaaggc gaagaacgtc
    10021 aagctcggcg cccaggcgca gaccaccggc gaggactacg tcaagaagga gggcttcgac
    10081 ccggtctcct tcgagtcctc cgacgccgtc ctcaacggcc tgcgcaccgg ccaggtcaag
    10141 gccgtcgtca tcgacttccc ggtggtccag ggctggctca aggacaaggc caccgccgac
    10201 gcctacgagg tcgtcgacaa cctcgacacc ggcgagcagt acggcttcac cgtgaagaag
    10261 ggcaacaccg cgctgcggaa ggccgtcaac aaggcactct cggacgccaa ggccgacggc
    10321 acgtacaaga agttgtacga gcagtggatc ggcccgtacg acgagtccgc cgccgaaccg
    10381 tccgccacct gatcccatg
//
