LOCUS       NZ_FMZK01000001        10504 bp    DNA     linear   CON 26-APR-2024
DEFINITION  Streptomyces prasinopilosus strain CGMCC 4.3504, whole genome
            shotgun sequence.
ACCESSION   NZ_FMZK01000001
VERSION     NZ_FMZK01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces prasinopilosus
  ORGANISM  Streptomyces prasinopilosus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900101585.1-RS_2024_04_26
            Annotation Date                   :: 04/26/2024 02:19:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,269
            CDSs (total)                      :: 6,190
            Genes (coding)                    :: 5,988
            CDSs (with protein)               :: 5,988
            Genes (RNA)                       :: 79
            rRNAs                             :: 5, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 202
            CDSs (without protein)            :: 202
            Pseudo Genes (ambiguous residues) :: 0 of 202
            Pseudo Genes (frameshifted)       :: 63 of 202
            Pseudo Genes (incomplete)         :: 175 of 202
            Pseudo Genes (internal stop)      :: 8 of 202
            Pseudo Genes (multiple problems)  :: 40 of 202
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:44:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 328460
            Orig. end                         :: 338964
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FMZK01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10504
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10504
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10504
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5504](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5504
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(188..1630)
                     /locus_tag="BLS36_RS01465"
                     /old_locus_tag="SAMN05216505_101290"
     CDS             complement(188..1630)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /GO_process="GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065681.1"
                     /locus_tag="BLS36_RS01465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101290"
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="WP_055570251.1"
                     /sec_met_domain="Bac_transf (E-value: 5.6e-60, bitscore:
                     198.0, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAEPTVTSPGVPPRDHGSSPVSVMPPRDTAVGPRLPGPLPAARP
                     ASPVPLFLADAAAALLGTLTLSGTQGYAPLAALLIGASLLLRPRGDRSPTAGPPGAGPL
                     EDLPAVCGRVAVAWLASAALWAAYRPEHALSAGAVLTGLALQSAAGGALRAAVHGRRRA
                     VLRRRPHAALVVGPAATAQRVAAALLRHPGCGVRPVGIVAGQPDGAGGLPVLRTGEEVE
                     RAVIQNGVRAVLAVHPSVRAGQGPLLHALAEAGCVVREIDADALPYPAEERLAGFPCRR
                     LDLSPARRGSAGKRALDVLVSGTLLLLTGPLLLLCALALRISDGPGVVFRQERIGRGGE
                     PFTLLKFRTHRPADAHESATRWSVADEHEMRRFCRMLRRTSLDELLQLWNVLRGDMSLV
                     GPRPERPYFVSQFSRTHPGYAARHRMRAGITGLAQVHGLRGDTSIEDRCRFDNAYIDDW
                     SLWQDVRILLRTAVTLVRPTGS"
     gene            complement(1627..2973)
                     /locus_tag="BLS36_RS01470"
                     /old_locus_tag="SAMN05216505_101291"
     CDS             complement(1627..2973)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527564.1"
                     /locus_tag="BLS36_RS01470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101291"
                     /product="glycosyltransferase"
                     /protein_id="WP_074993366.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 2.4e-31,
                     bitscore: 104.8, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTPAPVPDAAAGEGPARPDPPTGDRRPRVLHLTQPVDGGVARVV
                     TDLVRAQVAEGLDVTAVCPDSAFALHLRALGAQVRDWRSTRSPGPSLVREVRHLARVID
                     EVRPDLVHAHSAKAGLAGRLAVRGRIPTVFQPHAWSFEAVGGVTAALALGWERWGARWA
                     TRVVCVSEAERATGVRAGITGRWAVVPNGIDPERFRPAHAGTVRAGLAPLSALRPATPL
                     VVCVGRLCRQKGQDVLLRAWDTVVRRVPAARLVLVGDGPDDGLLRARAPGSVLFAGAVT
                     DASPWYRAADLVVLPSRWEGMALAPLEAMACGRPVVVTDVDGARESLPPALAPHCLVPP
                     EDPKALAGALSGLLLDPPLRESLGDVGRRHVLSVHDVRHTAEQIAGVYRDLLGARPSGG
                     RARPSRAVERGGARGTGRGQPPDAGRRTEAANRTTQRVEPTKYRETIRS"
     gene            complement(2970..4067)
                     /locus_tag="BLS36_RS01475"
                     /old_locus_tag="SAMN05216505_101292"
     CDS             complement(2970..4067)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019056483.1"
                     /locus_tag="BLS36_RS01475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101292"
                     /product="hypothetical protein"
                     /protein_id="WP_055570250.1"
                     /transl_table=11
                     /translation="MRHSLGQLLRPVTVGVLALVAMWAPCGAPAAAPAAPPAPEASGLA
                     FGQRYHALQHGGIVRAANSSATCRPAGSSSCADAQAGARAVNGDFDMSYVDVDRDANTY
                     NSSRAEVRLPSGSRVTYARLYWGGNLRVGEQKPAEDNGRVLIAEPGGEYKELLADTVVG
                     HDVADGADAFQASADVTRLVRDSGAGLYTVAQVNVAAGRSAAGAWGGWTLVVAYENPAE
                     PLRHLSLWDGFAPLRSRTGPEVGLTGPDLPEGAGGQVGLVAYNGDRGTTGDSLTVTTGT
                     PASTATLTDSANPAGDVLNSTVSGPAGAVPARVPAYANTLGYDSDVFTLGRGVLRAGDR
                     MSLRLSSQRDAAWAGVLFAAVDARR"
     misc_feature    complement(4111..4125)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.73"
                     /tool="antismash"
     misc_feature    4254..4268
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.41"
                     /tool="antismash"
     gene            4596..4868
                     /locus_tag="BLS36_RS01480"
                     /old_locus_tag="SAMN05216505_101293"
     CDS             4596..4868
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987888.1"
                     /locus_tag="BLS36_RS01480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101293"
                     /product="hypothetical protein"
                     /protein_id="WP_055570249.1"
                     /transl_table=11
                     /translation="MKSLKAAAVIAGSLIAAGMAAPAYAQEVDLASTGLDTGLNTRTAV
                     PFELMSPQEESSADESRTTVFGTAEEAADVVNQAKPVRGDLGLHA"
     gene            5001..5504
                     /locus_tag="BLS36_RS01485"
                     /old_locus_tag="SAMN05216505_101294"
     CDS             5001..5504
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826201.1"
                     /locus_tag="BLS36_RS01485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101294"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_055570248.1"
                     /sec_met_domain="melC (E-value: 2.4e-48, bitscore: 160.0,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVPIDPAPTGAGDTAPRPAPPGPAGRTRRDVARGLRAGALAGAL
                     APVLAAFRSPRPGPAETDPPGGAAPAFAQTYRGRRLRGVWTPGHTAAEDGRWHVTVDGR
                     PLHLMRRADGGWLSTVDHYCSYRTPLEAARAAVDALGPGERLRDAAPGDTGHAHGEGRH
                     GVRA"
     gene            5488..6354
                     /locus_tag="BLS36_RS01490"
                     /old_locus_tag="SAMN05216505_101295"
     CDS             5488..6354
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="BLS36_RS01490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101295"
                     /product="tyrosinase family protein"
                     /protein_id="WP_055570247.1"
                     /transl_table=11
                     /translation="MVYVRKDAGTLTAAEKRRFVKALLEVKRRGEYDEFVRMHLAYFSS
                     DGERGLRTAHMTPSFLPWHRRFLLDLETALRRVDPSVTLPYWDWTRDRTATSTPWTADF
                     LGGDGRRSDRQVMTGPFAHRQGDWTIEVGVTDGEFLTRDLGRRREPLDLPTASDLERAL
                     RDPVYDAAPWDSTVTRGFRNKLEGWGTGQGGAAWRNHNRVHRWVGGAMLGAASVNDPVF
                     WLHHAFVDLQWYRWQRRHGGARYLPATPPGPRDGQHRRVVARHEKLPPWDVTPDELEDV
                     GRIYRYA"
     misc_feature    complement(6517..6535)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.42"
                     /tool="antismash"
     gene            6589..6858
                     /locus_tag="BLS36_RS01495"
                     /old_locus_tag="SAMN05216505_101296"
     CDS             6589..6858
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527571.1"
                     /locus_tag="BLS36_RS01495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101296"
                     /product="hypothetical protein"
                     /protein_id="WP_055570246.1"
                     /transl_table=11
                     /translation="MRKYQKAAVVMAMLGSVGFLGAGVSHAGDDPSFKLNNEQTTACAQ
                     DNSVQGLVNIDDVNAGVGAILGLGNAENTETNAVACASSFTFGG"
     gene            7001..7279
                     /locus_tag="BLS36_RS01500"
                     /old_locus_tag="SAMN05216505_101297"
     CDS             7001..7279
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="BLS36_RS01500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SAMN05216505_101297"
                     /product="hypothetical protein"
                     /protein_id="WP_055570245.1"
                     /transl_table=11
                     /translation="MLEPKRIATAVGLLAGVALIGVGAVRTVGAEDSGDCAGSGGSGVR
                     CERVREYRLPADLQGEVRFTGRQEQDCSGSDVEVSCVNGTLLGGERV"
     misc_feature    complement(7305..7318)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.15"
                     /tool="antismash"
     misc_feature    complement(7305..7319)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.61"
                     /tool="antismash"
     misc_feature    7306..7319
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.17"
                     /tool="antismash"
     misc_feature    complement(7306..7320)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.78"
                     /tool="antismash"
     misc_feature    complement(7307..7320)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.93"
                     /tool="antismash"
     misc_feature    complement(7308..7321)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.05"
                     /tool="antismash"
     misc_feature    complement(7311..7326)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.99"
                     /tool="antismash"
     misc_feature    complement(7313..7322)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.63"
                     /tool="antismash"
     misc_feature    complement(7346..7360)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.3"
                     /tool="antismash"
     misc_feature    7397..7410
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     gene            7427..7693
                     /locus_tag="BLS36_RS01505"
                     /old_locus_tag="SAMN05216505_101298"
     CDS             7427..7693
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527571.1"
                     /locus_tag="BLS36_RS01505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101298"
                     /product="hypothetical protein"
                     /protein_id="WP_055570244.1"
                     /transl_table=11
                     /translation="MRKYQKAAVVMAMLGSAGFLGAGVGHASDGPEVELNNEQTTTCVQ
                     DNSKQGLINIDDINLAVGILGLGNAENTETNAVSCTSSLAIGG"
     gene            7836..8114
                     /locus_tag="BLS36_RS01510"
                     /old_locus_tag="SAMN05216505_101299"
     CDS             7836..8114
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987900.1"
                     /locus_tag="BLS36_RS01510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101299"
                     /product="hypothetical protein"
                     /protein_id="WP_055570243.1"
                     /transl_table=11
                     /translation="MVKSSKFAAVAGVLGGLALIGAGSVQAFGAEDPDNCVEDEKGTIR
                     CVQVSEQEITTDQYGKIHFVNNSKQTCSGENAEVSCTNSVVLPDEKS"
     gene            complement(8240..9217)
                     /locus_tag="BLS36_RS01515"
                     /old_locus_tag="SAMN05216505_101300"
     CDS             complement(8240..9217)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (53-281). E-value: 4e-21.
                     Score: 67.7. Matches aSDomain:
                     nrpspksdomains_BLS36_RS01515_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931241.1"
                     /locus_tag="BLS36_RS01515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101300"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_055570242.1"
                     /sec_met_domain="PF00561 (E-value: 3.3e-17, bitscore: 59.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.4e-12, bitscore:
                     44.1, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTALTAPQGSPNTQRHGFAPPPPEQVVEERTLVFEGFAYECRIV
                     HQDTPRTAPLLLLGGSSQNRHSWQGHEKWLAPLCTVITVDLPGYGSADFLPARYGIDFL
                     AAAVQHVLVETGMPPVNLFGGCFGEVVGLRFAQLHPGSVRRIVFCGAAMRLPEIYTDAM
                     PRLARALERGETEAAAGGLVELFMSNPAAGQVRRHAAVARLLHRQFMNQTPDELGKAVE
                     HNTRLVTHGCYEPLPVPDVPTLVFTGEHDTLCTPQMGRELAATIPAARFTTVKEADHLV
                     TVERRQESSELIGRFCTDRPIDDLPYCTPVESPGAGLARATAHR"
     aSDomain        complement(8375..9058)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLS36_RS01515_Abhydrolase_1.1"
                     /evalue="4.00E-21"
                     /label="BLS36_RS01515_Abhydrolase_1.1"
                     /locus_tag="BLS36_RS01515"
                     /protein_end="281"
                     /protein_start="53"
                     /score="67.7"
                     /tool="antismash"
                     /translation="PLLLLGGSSQNRHSWQGHEKWLAPLCTVITVDLPGYGSADFLPAR
                     YGIDFLAAAVQHVLVETGMPPVNLFGGCFGEVVGLRFAQLHPGSVRRIVFCGAAMRLPE
                     IYTDAMPRLARALERGETEAAAGGLVELFMSNPAAGQVRRHAAVARLLHRQFMNQTPDE
                     LGKAVEHNTRLVTHGCYEPLPVPDVPTLVFTGEHDTLCTPQMGRELAATIPAARFTTVK
                     EADHLV"
     misc_feature    complement(9276..9291)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 20.95"
                     /tool="antismash"
     gene            complement(9470..9751)
                     /locus_tag="BLS36_RS01520"
                     /old_locus_tag="SAMN05216505_101301"
     CDS             complement(9470..9751)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132942.1"
                     /locus_tag="BLS36_RS01520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05216505_101301"
                     /product="chaplin"
                     /protein_id="WP_055570241.1"
                     /transl_table=11
                     /translation="MSRIAKAACVALGTGAVALSGAGTALADAGAQGVASHSPGVLSGN
                     LVQAPVHVPVNVCGNTVNVIGALNPAFGNTCVNEDGQGHKNHHDAYGN"
ORIGIN
        1 cgcagcaggc ggaccgcgca gtacaccacc accagtgcgg agaccacgtc cgcgggcccc
       61 gctccgccgt gcgtcaccgg cagggccagc agggcgggca gcgccaccag gggcagcacc
      121 ggcaccagcc cggcggcccg gcgggcgaac gggagcggga gcggtgcggg agcgaggctc
      181 acgtcggtca gctccccgtc ggacggacca gcgtgaccgc ggtgcgcagc aggatgcgga
      241 cgtcctgcca cagcgaccag tcgtcgatgt aggcgttgtc gaaccggcag cggtcctcga
      301 tcgaggtgtc gccgcgcagg ccgtggacct gggcgagacc ggtgatgccg gcccgcatcc
      361 ggtgccgggc cgcgtaaccg ggatgggtcc ggctgaactg ggagacgaag taggggcgtt
      421 cgggccgcgg cccgaccagg ctcatgtcgc cgcggagcac gttccacagc tggagcagtt
      481 cgtcgagcga ggtgcgccgc agcatgcggc agaagcggcg catctcgtgc tcgtcggcca
      541 cgctccaccg ggtcgccgac tcgtgcgcgt cggccggccg gtgcgtacgg aacttcagca
      601 gcgtgaacgg ctcgccgccc ctgccgatgc gctcctgccg gaacaccacg cccggcccgt
      661 cgctgatccg cagcgccagc gcgcacagca gcagcagcgg accggtcagc agcagcagcg
      721 tccccgacac caggacgtcg agcgcgcgct tgcccgcact gccgcggcgc gccggcgaca
      781 ggtccaggcg gcggcacggg aaaccggcca gccgttcctc cgccgggtac ggcagggcgt
      841 cggcgtcgat ctcccggacc acgcagcccg cctcggccag cgcgtgcagc agcgggccct
      901 gtccggcgcg taccgagggg tggacggcca gcacggcccg caccccgttc tggatgaccg
      961 cccgctcgac ctcctcaccg gtgcgcagca ccggcaggcc cccggcgccg tccggctgcc
     1021 cggcgacgat ccccaccggc cgcaccccgc accccgggtg ccgcagcagg gcggcggcca
     1081 cgcgctgcgc ggtcgccgcc gggccgacga cgagcgccgc gtgcgggcgg cgccgcagca
     1141 cggcgcgccg ccgcccgtgc acggccgcgc gcagcgcgcc gccggccgcc gactgcaggg
     1201 ccagaccggt cagcacggcg ccggcggaca gcgcgtgctc cggccggtac gccgcccaga
     1261 gcgccgcgga cgccagccag gccaccgcga cccggccgca cacggcgggc aggtcctcca
     1321 gcggccccgc tcccggcggc cccgccgtcg gcgaccggtc gccgcgcggg cgcagcagca
     1381 gcgacgcgcc gatcagcagg gcggcgagcg gggcgtaccc ctgggtgccg gacagcgtca
     1441 gcgtgcccag cagggccgcg gcggcgtccg cgaggaacag cggtacgggc gaggccggcc
     1501 gtgccgcagg aagggggccg ggcagccgcg gaccgacggc ggtgtcgcgc ggcggcatga
     1561 cggagacggg tgaggatccg tggtcccgcg gcggtacgcc gggggaggtc acggtgggtt
     1621 cggcggtcac gagcggatgg tctccctgta cttggtgggc tcgacgcgct gcgtggtccg
     1681 gttcgccgct tcggtgcggc ggcccgcgtc gggcggctgc ccccgcccgg tgccgcgggc
     1741 cccgccacgc tcgacggccc gggacggacg cgccctcccc ccggagggcc gggcgccgag
     1801 cagatcgcgg tagacgcccg cgatctgctc ggcggtgtgc cgtacgtcgt ggacggacag
     1861 cacgtgccga cggcccacgt cgccgagcga ctcgcgcagc ggcgggtcga gcagcagccc
     1921 ggacagggca ccggccaggg cctttggatc ctccggcggt accaggcagt ggggggcgag
     1981 ggccggcggc aggctctcgc gggcgccgtc gacgtccgtc accaccacgg gacggccgca
     2041 ggccatcgcc tccagcgggg cgagggccat gccctcccag cgggacggca ggacgacgag
     2101 gtcggcggcc cggtaccagg gcgaggcgtc ggtgacggct cccgcgaaca gcacggaccc
     2161 gggggcccgc gcgcgcagca ggccgtcgtc cggtccgtcg cccaccagga cgaggcgtgc
     2221 ggcgggcacc cgccgcacga cggtgtccca cgcccgcagc aggacgtcct gccccttctg
     2281 ccggcagagc cgccccacgc agaccacgag cggtgtcgcg gggcggaggg cggagagcgg
     2341 ggcgagcccg gcccgcacgg tgccggcgtg ggccgggcgg aagcgctcgg ggtcgatgcc
     2401 gttggggacg acggcccacc gtccggtgat cccggcgcgc acaccggtcg cccgttccgc
     2461 ctcgctcacg cacaccaccc gcgtggccca gcgcgccccc caccgttccc acccgagcgc
     2521 gagcgccgcg gtgaccccgc ccaccgcctc gaacgaccag gcgtgcggct ggaagacggt
     2581 cgggatccgg ccgcgcaccg cgagccgtcc ggcgagcccg gccttcgcgc tgtgcgcgtg
     2641 caccaggtcg gggcgcacct cgtcgatcac ccgcgccagg tgccgcacct cccgcacgag
     2701 cgacgggccg ggcgaccgcg tggaccgcca gtcgcgcacc tgcgcgccga gcgcccgcag
     2761 gtggagggcg aacgcgctgt cggggcagac cgcggtgacg tccaggccct ccgccacctg
     2821 cgcgcggacc aggtccgtca ccacgcgcgc gaccccgccg tccaccggct gggtgaggtg
     2881 cagcacccgc ggccggcggt cgccggtcgg cgggtccggg cgtgcgggtc cctcacccgc
     2941 ggcggcgtcc gggacgggtg ccggcgtgct catcgccggg cgtcgacggc ggcgaagagc
     3001 acgccggccc aggccgcgtc ccgctgggac gagagccgca ggctcatccg gtcgccggcg
     3061 cgcagcacac cgcgtccgag ggtgaacacg tcggagtcgt agccgagcgt gttggcgtac
     3121 gccggcaccc gcgccggaac ggccccggcg ggcccgctga cggtggagtt caggacgtcg
     3181 ccggcggggt tggcggagtc ggtgagtgtc gcggtggagg cgggcgtgcc ggtggtcacg
     3241 gtgagcgagt cgcccgtggt gccgcgatcg ccgttgtacg ccaccagtcc cacctggccc
     3301 cccgcgccct ccgggaggtc cgggccggtg agcccgacct cgggtccggt gcgggagcgc
     3361 agcggcgcga agccgtccca gagcgagagg tgccgcagcg gctccgccgg gttctcgtac
     3421 gccaccacca gggtccagcc gccccacgcg ccggccgccg aacggccggc ggccacgttg
     3481 acctgcgcca cggtgtacag gcccgccccg ctgtcccgca ccagccgggt cacgtccgcc
     3541 gaggcctgga aggcgtcggc gccgtccgcc acgtcgtgtc ccacgacggt gtccgcgagg
     3601 agctccttgt actccccgcc gggttcggcg atcaggaccc gcccgttgtc ctccgcgggc
     3661 ttctgctcgc ccacgcgcag gttgccgccc cagtacaggc gcgcgtacgt cacccgggag
     3721 ccggagggca gacggacctc cgcgcgggag gagttgtagg tgttcgcgtc gcggtcgacg
     3781 tcgacgtagg acatgtcgaa gtcgccgttc accgcccgcg cgcccgcctg cgcgtcggcg
     3841 cacgaggacg acccggccgg gcggcaggtg gcggaggagt tggccgcgcg cacgatgccc
     3901 ccgtgctgga gcgcgtgata ccgctggccg aacgcgagac cgctcgcctc cggcgcgggc
     3961 ggcgcggccg gagcggcggc cggagcgccg cagggcgccc acatcgcgac gagggcgagg
     4021 acgcccacgg tgacgggacg gagcaactga cccagggaat ggcgcatgac cgtcggagcc
     4081 tttcggaacg ggtggcaggg gccggatggg tgaagacgac gtccgcggcc gccggtgcct
     4141 cgcccgagga cggcgacgac gagcgggacc gccgggacgg agcaggcagc acgaggagtg
     4201 cgatgcgtcc gctcctgggg tgcgtcggcg caactctagc caccggccgg acaaatttgc
     4261 cgaaactcaa tcaacgccga caagtgtgtt ggaaacgtga aaccgatccg cgcgaagtgg
     4321 cacccgggtg gtgcagcccc gtcgtcccgt cgaccggatc agggccgtac gggtgaaaag
     4381 gggacgccga aggcgtgccg cgccgctcgc gcggcacggc aaccccggcg tccgcgttca
     4441 ccgttccgcc ggcgccgatg acgccaccgc tcttctgatc tcatatgacg actcgtcaat
     4501 ctgtggttcc ccggtttccg cacgggccga ggatcgttga gcacggcgac acaaagcccc
     4561 ctccggcgac ccgcgttgcc gaagaaagga acacgatgaa gtccctgaag gctgccgccg
     4621 tcattgccgg gtccctgatc gccgccggta tggccgcgcc cgcgtacgcg caggaggtcg
     4681 acctggcgtc caccggcctc gacaccggtc tgaacacgcg caccgcggtc cccttcgaac
     4741 tcatgtcgcc gcaggaggag tcgagcgccg acgagtcccg gacgacggtg ttcggcaccg
     4801 cggaggaggc ggcggacgtg gtcaaccagg ccaagccggt gcgcggcgat ctggggctgc
     4861 acgcctgaga cgcgccgtac gaagtcggcc ccccggtccg gaccgggggg ccgacttcgt
     4921 cgttcccccg ccgtgggagg tgaccacacg gcgggaccga cggttggaac ggcgacagcc
     4981 gttcggacaa ggagtggatc atgaatgttc ccattgaccc ggcgcccacg ggcgccgggg
     5041 acacggcgcc gcgcccggcg ccgcccggcc cggcgggcag aacacgacgg gacgtggcac
     5101 ggggcctgcg ggccggcgcc ctcgccggcg ccctggctcc ggtgctcgcc gccttccggt
     5161 ctccgcgccc cgggcccgcg gagacggacc ccccgggcgg tgcagccccc gccttcgcgc
     5221 agacctaccg cggccgccgc ctccggggag tgtggacgcc ggggcacacc gcggccgagg
     5281 acggccggtg gcacgtcacg gtggacggcc gtcccctgca cctgatgcgc cgcgccgacg
     5341 gcggctggct gagcacggtc gaccactact gctcctaccg gacaccgctc gaggccgccc
     5401 gcgcggccgt cgacgcgctc ggccccggcg agcggctgcg tgacgcggcg ccgggggaca
     5461 cgggacacgc gcacggggag ggccggcatg gtgtacgtgc gtaaggacgc cggcacgctc
     5521 acggcggccg agaagcgacg cttcgtcaag gcgctgctgg aggtcaaacg acggggcgag
     5581 tacgacgagt tcgtgcggat gcacctcgcg tacttctcct ccgacggcga gcgcggactg
     5641 cgcaccgcgc acatgacgcc ctccttcctg ccctggcacc gccggttcct gctggacctg
     5701 gagaccgccc tgcgccgggt cgacccgtcg gtgaccctgc cgtactggga ctggacccgg
     5761 gaccgcacgg ccacctccac gccgtggacg gcggacttcc tcggcggcga cgggcgccgg
     5821 tccgaccggc aggtgatgac ggggccgttc gcgcaccggc agggcgactg gaccatcgaa
     5881 gtgggcgtga ccgacggaga gttcctcacc cgggacctcg gccgccgccg cgaaccgctc
     5941 gacctgccca ccgcgagcga cctggagcgg gcgctgaggg accccgtcta cgacgcggct
     6001 ccctgggact cgacggtcac gcggggcttc cgcaacaagc tggagggctg gggcaccggc
     6061 cagggcggcg cggcctggcg caaccacaac cgggtgcacc gctgggtcgg cggggccatg
     6121 ctcggcgccg cctccgtcaa cgaccccgtc ttctggctgc accacgcctt cgtcgacctg
     6181 cagtggtacc gctggcagcg ccggcacggc ggggcccgct acctccccgc gacaccgccg
     6241 ggcccccgcg acgggcagca ccgccgggtc gtggcacggc acgagaagct gccgccctgg
     6301 gacgtgacgc cggacgaact ggaggacgtc ggccggatct accggtacgc ctgaggcggc
     6361 accggaaccg gctgcacgag aaggacctcg gcgttccggg cgccggggcg gacacacgcc
     6421 ttcggagcag tcgacacgcg tggacaggcc cgtgacgtgc gccttcgcaa gaaatttacg
     6481 cacatgggtg ttttgactgt ccaaagccgt agacgtcgcc tacgttatgc actgtcggtg
     6541 accgacccaa cacgggttgt gtcaacttct gaaatcggag aagaagcaat gcgcaagtac
     6601 cagaaggctg cagtcgtcat ggccatgctc ggcagcgtcg ggttcctcgg cgccggcgtc
     6661 agccacgccg gagacgaccc gtcgttcaag ctcaacaacg agcagaccac ggcctgcgcc
     6721 caggacaact cggttcaggg cctcgtcaac atcgacgacg tcaacgccgg tgtcggcgcc
     6781 atcctgggcc tcggcaacgc ggagaacacc gagaccaacg cggtggcctg cgcctccagc
     6841 ttcaccttcg gcggctgatc accttcaggt gatccaggtg ggtcccgggg ctcgaaccgc
     6901 gcgttcgggc tccgggaccg cccggcacgc cggccggccc ggacctccgg gccggtcccg
     6961 ccgccggacc gcggcggggc gaccgtcagg gaaaaggcag atgctcgagc cgaagaggat
     7021 cgcaacggcg gtggggctcc tggccggtgt cgccctgatc ggcgtcggtg ccgtccggac
     7081 tgtcggcgcc gaggactccg gcgactgtgc cgggagcggt gggagcggcg tccgctgcga
     7141 gcgggtgagg gagtaccgcc tcccggccga cctgcagggg gaggtccgct tcaccggccg
     7201 gcaggagcag gactgctccg gttcggatgt cgaggtctcc tgcgtgaacg gcaccctcct
     7261 cggcggcgag agggtctgag gccccctcgc ccgggcgtga tgccgatttg ttgaaaaaaa
     7321 cgcgcgatac ggcgtttcgt ctaccaattg ccggaatcgt cgcctagatt agcgactgta
     7381 agtgaccgga ccatcacgga ccgtgtcaaa tcagaaggag aagttaatgc gcaagtacca
     7441 gaaggctgcg gtcgtcatgg ccatgctcgg cagtgccgga ttcctcggcg ccggcgtcgg
     7501 tcacgccagc gacgggcccg aggtcgagct caacaacgag cagaccacga cctgcgtgca
     7561 ggacaactcg aagcagggtc tgatcaacat cgacgacatc aacctggcgg tcggcatcct
     7621 gggcctcggc aacgcggaga acaccgagac caacgccgtc tcctgcacct ccagcctcgc
     7681 catcggcggc tgatcaccct cacaggtgat ccgggggccc ggggactcga gccacgcgtt
     7741 cgagccccgg gcccgcccgg cacattggcc ggcccggatg tccgggccgg cttcgccgct
     7801 cactcgcggc acgttgaccg tcaaggaaaa ggtagatggt gaagtcgagc aagttcgcag
     7861 cggtggcggg ggtcctcggt gggctcgcgc tgatcggcgc cggttccgtc caggctttcg
     7921 gcgccgagga cccggacaac tgcgtcgagg acgaaaaggg cacgattcgc tgcgtgcagg
     7981 tgagcgagca ggagatcacg accgaccagt acgggaagat ccacttcgtc aacaactcga
     8041 agcagacctg ctccggcgag aacgccgagg tctcctgcac caacagcgtc gtcctcccgg
     8101 acgagaagtc ctgaactctc cgacggtcga gtgccgttgc ccgcggcacc cctcacgtcg
     8161 gcattccgct tcggatcatc cgtacggcgg agagtggccg gaacccggta ccgggttccg
     8221 gccactcgtc gtgtgtccgt caccggtggg ccgtcgcgcg ggcgaggccg gcgccgggcg
     8281 actccacggg agtgcagtac ggcaggtcgt cgatggggcg gtcggtgcag aagcggccga
     8341 tcagctccga cgactcctgc cgtcgctcga ccgtgaccag gtggtccgcc tccttgaccg
     8401 tggtgaaccg cgccgccgga atcgtcgcgg cgagctcccg gcccatctgc ggggtgcaca
     8461 gggtgtcgtg ttcgccggtg aacacgagcg tcggcacgtc ggggacgggc aggggctcgt
     8521 aacagccgtg cgtgaccagt cgcgtgttgt gctcgacggc cttcccgagc tcgtccggcg
     8581 tctggttcat gaactgacgg tgcaggaggc gggcgacggc cgcgtgccgg cggacctggc
     8641 ccgcggccgg attcgacatg aacagctcca ccaggccgcc ggccgccgcc tcggtctcgc
     8701 cccgttccag cgcgcgcgcc aagcggggca tcgcgtcggt gtagatctcg gggaggcgca
     8761 tcgcggcgcc gcagaagacg atgcggcgga cggatccggg gtgcagctgc gcgaagcgga
     8821 gaccgacgac ttccccgaag cagccgccga agaggttcac cggcggcatc ccggtctcca
     8881 ccagcacgtg ctggaccgcg gcggcgagga aatcgatccc gtagcgggcg ggcaggaagt
     8941 ccgcactgcc gtacccgggg aggtccacgg tgatcaccgt gcacaacggc gccagccact
     9001 tctcgtgccc ctgccaggag tgccggttct gggacgagcc tccgagcagc agcagcggcg
     9061 ccgttcgcgg ggtgtcctgg tggacgatcc ggcactcgta cgcgaagccc tcgaacacca
     9121 gggtccgctc ctccaccacc tgctccggcg gtgggggcgc gaacccgtgg cgttgcgtat
     9181 tcggtgaacc ctggggggcg gtgagggcag tgcgcatgga cggctccggt acaggggaaa
     9241 aggcgcgaac cagtccatca ttaccagcaa tcacggtttt catccgtaat cactcatttg
     9301 tgggcgatcc cggcggaagg gcatggccgc gcgccggacg gcgacgccgg ggatcccccc
     9361 tcgaccccgg gggccggggg ttcggggacg gtgccgggaa cggcgagagc cccggcgctc
     9421 gaggtgccgg ggctctggac gtcctgctga tggtgacgcg taggaggggt cagttcccgt
     9481 acgcgtcgtg gtggttcttg tgcccctggc cgtcctcgtt cacgcaggtg ttgccgaacg
     9541 ccgggttcag cgcgccgatg acgttgacgg tgttcccgca cacgttgacc ggcacgtgga
     9601 ccggggcctg gacgaggttg cccgacagca ctcccgggga gtgggaggcg acgccctggg
     9661 cacccgcgtc cgccagagcc gtaccggccc cgctcagggc cacggcaccc gtgccgagtg
     9721 cgacgcaggc ggccttcgcg atgcgcgaca tcacgttctc cttcttgttc gatgagcacg
     9781 gacgcagggg gtgcggccgt gcttcccgtt caacgcggag gggcgcgacg ggtcacggtg
     9841 atcggccgtg gatcacccgg ggcgggggag ttttcttccg gccggccttc cggtcaggtc
     9901 ttccggctgc tctcagggcg gacgccggac cggtgcgccg tcgaggaaag cgcggatgtc
     9961 ctcgaccgcg tgatcgggcg actcttcctg cggccggtgc ccgtgctggg gtgccggcgt
    10021 ccggggggaa ggcggggcat gcgtccgcgg agcctcgtgc tcggcacctc acccctgcgg
    10081 agggggtctt cgacctgccc gccgtcatcg tcccggaccc ggtcgagcgg gacgccgatg
    10141 gcagtcccga cactctgggg ggtcggcgga gggcttccgg gggagaggct tccggaagga
    10201 gagggcggac cgccggggcg gggatcagca gtggtacgac tgcacgacgt cgtcgaagcg
    10261 tcccgccagc aggtcgtcgc ggttgacgag ccagcccacc tcgccgtccc cggtgtggaa
    10321 gatcgcgtcc tcgccgaact ccaggaggtt cagccacggt ccgccgtcct gctcggaggc
    10381 gtcccgcagg gcctcggcgt acagctcgtc gaaccgctcc ggctcgttcc ggcagacgac
    10441 cacggcctcc tcccccttct tcaggaagtc cgttccgtag tcggcccggt agtacggggg
    10501 attc
//
