LOCUS       NZ_FNDV01000014        10402 bp    DNA     linear   CON 11-JAN-2024
DEFINITION  Actinokineospora alba strain CPCC 201030, whole genome shotgun
            sequence.
ACCESSION   NZ_FNDV01000014
VERSION     NZ_FNDV01000014.1
KEYWORDS    WGS; RefSeq.
SOURCE      Actinokineospora alba
  ORGANISM  Actinokineospora alba
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Actinokineospora.
REFERENCE   1
  AUTHORS   Varghese,N. and Submissions,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2016) DOE - JOINT GENOME INSTITUTE, LBNL, 2800
            Mitchell Drive, Walnut Creek, CA 94598, 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 04:12:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,667
            CDSs (total)                      :: 6,588
            Genes (coding)                    :: 6,533
            CDSs (with protein)               :: 6,533
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 14 of 55
            Pseudo Genes (incomplete)         :: 41 of 55
            Pseudo Genes (internal stop)      :: 4 of 55
            Pseudo Genes (multiple problems)  :: 4 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:41:36
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 111293
            Orig. end                         :: 121695
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FNDV01000014.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10402
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10402
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10402
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5402](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5402
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            24..791
                     /locus_tag="BLR56_RS30375"
                     /old_locus_tag="SAMN05421871_114102"
     CDS             24..791
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230325.1"
                     /locus_tag="BLR56_RS30375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421871_114102"
                     /product="Abi-alpha family protein"
                     /protein_id="WP_228770073.1"
                     /transl_table=11
                     /translation="MDDDRRALASANGSDPTAEMVDRAGRALGWLYRSGWQVARRLPGG
                     EVAERQVQKVEEAIVAEVRKHLEPLPDPEPRQIEAARVREPMRLAMAQLLERSVTDSKD
                     AARDHYFESVLRQLLPDEARIVSALSDGTVFPLMHLAARTARGGAPRFVLENASTVGRV
                     AGVVQPGLVPLYVGRLLRFGLVEVGEEDPTLGVAYEMLLTDDRVRAAEDRAKAQSRFGA
                     KAIRLTLRISELGAQFWAVCDPSARGLTLGERR"
     gene            788..2023
                     /locus_tag="BLR56_RS30380"
                     /old_locus_tag="SAMN05421871_114103"
     CDS             788..2023
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084084.1"
                     /locus_tag="BLR56_RS30380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421871_114103"
                     /product="DUF445 domain-containing protein"
                     /protein_id="WP_091379694.1"
                     /transl_table=11
                     /translation="MNAMLQDFQTNWMVYASMPLIAALIGYVTKLVAIRMMFQPLEFVG
                     KKPYLGWQGIVPRRAARMASIAVDTMTDRLISASEVMGRLDPERVVKEIEQPLLAGVEE
                     IAAEIAQEFQPGLWENLPEQVRSRIVRHIQAEAPGVVASLLETVKNDVDAVFDLKDMVV
                     TNLVRDKALLNRIFQHAGRKEFQFIARSGIVFGFVIGFVQMFAWAALKNPWVMPIFGGL
                     TGWFTDWLALKMIFNPKKPTRYLGIFEWQGLFLKRRKEVAADYGDLIAREIITPKNVME
                     AVLRGPLSDRLFALVAAQVRTVIDKQSGPARPLVMLAVGSGRYQAIKNSIAEKVIARLP
                     ETMTHIESYAEDAMDIRNTLVTKMQEMSEDEFEELIRPAFEQDEWILITVGAVLGFCVG
                     ELQVILVENLAH"
     gene            2070..3098
                     /gene="mgrA"
                     /locus_tag="BLR56_RS30385"
                     /old_locus_tag="SAMN05421871_114104"
     CDS             2070..3098
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="mgrA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080305.1"
                     /locus_tag="BLR56_RS30385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421871_114104"
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="WP_091379691.1"
                     /transl_table=11
                     /translation="MAYVASDDRYDRIPYRRCGRSGLKLPTVSLGLWHNFGDDQPHEIG
                     RTILRRAFDLGVTHFDLANNYGPPYGSAERNFGRALEREFGAYRDELVISTKAGYDMWP
                     GPYGEWGSRKYLLSSLDQSLKRMGLDYVDIFYSHRFDPDTPLEETVGALASAVQQGKAL
                     YVGVSSYSPTATRQAAELLRAAGVPLLIHQPSYSMLNRWIEDELLDALGDLGVGCIAFS
                     PLAQGLLTDKYLDGVPSGSRGSRDSSLLPEMLSEDNLRRVRGLNEIAKSRGQTLAQLAV
                     SWVVRDDRVTSALVGASSVEQLESTLRAVDGPPFTDDELAEIDRYAVDSGIDLWESSRS
                     SG"
     gene            3239..3748
                     /gene="ectA"
                     /locus_tag="BLR56_RS30390"
                     /old_locus_tag="SAMN05421871_114105"
     CDS             3239..3748
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669882.1"
                     /locus_tag="BLR56_RS30390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421871_114105"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_091379688.1"
                     /transl_table=11
                     /translation="MSGKRVREEIGAPSIADGAAIWRIARDSRALDLNSSYAYLLWCRD
                     FAQTSAVARIDGQVVGFVIGFLRPECPDTVVVWQIAVDESQRGSGLGGRLLDHLMARVA
                     AQGVCRLETTITADNAASIGLFASLARRHHARLGRSELFSADHFPDDHEAEDLYRIDFP
                     NGDGEQ"
     gene            3745..5004
                     /gene="ectB"
                     /locus_tag="BLR56_RS30395"
                     /old_locus_tag="SAMN05421871_114106"
     CDS             3745..5004
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-358). E-value: 3.9e-71.
                     Score: 231.7. Matches aSDomain:
                     nrpspksdomains_BLR56_RS30395_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985333.1"
                     /locus_tag="BLR56_RS30395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421871_114106"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_091379687.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.6e-74, bitscore:
                     244.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVFDMIESEVRSYCRSWPVTFDRATGSRIFDENGQGYLDFFAGA
                     GALNYGHNNPILKQAVIDYIQRDGVTHSLDMQTVAKRDFLETFSETVLAPRELDYKIMF
                     PGPAGANAVEAALKLARKVTGRESIINFTNAFHGMTLGALSVTGNSLKRGGAGVPLVHA
                     TPMPFDNYLDGLTPDFMLFERLLKDSGSGLNEPAAVIVETVQGEGGINVASVEWLRSLS
                     ELCQRQGILLIVDDVQMGCGRTGPFFSFEAAGIKPDIVCLSKSIGGYGLPLGLTLLRPE
                     LDVWEPGEHNGTFRGINPAFVASTEALKTYWTDDTLEKSTLAKGEQVGRALTDLALAYP
                     EAALKAKGRGLARGLQFADGDIAGKVCRAAFDRGLLMETSGPDGEVAKIMPPLTVTDAE
                     LEEGLAIVDDAVAATLGVLR"
     aSDomain        3811..4818
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BLR56_RS30395_Aminotran_3.1"
                     /evalue="3.90E-71"
                     /label="BLR56_RS30395_Aminotran_3.1"
                     /locus_tag="BLR56_RS30395"
                     /protein_end="358"
                     /protein_start="22"
                     /score="231.7"
                     /tool="antismash"
                     /translation="DRATGSRIFDENGQGYLDFFAGAGALNYGHNNPILKQAVIDYIQR
                     DGVTHSLDMQTVAKRDFLETFSETVLAPRELDYKIMFPGPAGANAVEAALKLARKVTGR
                     ESIINFTNAFHGMTLGALSVTGNSLKRGGAGVPLVHATPMPFDNYLDGLTPDFMLFERL
                     LKDSGSGLNEPAAVIVETVQGEGGINVASVEWLRSLSELCQRQGILLIVDDVQMGCGRT
                     GPFFSFEAAGIKPDIVCLSKSIGGYGLPLGLTLLRPELDVWEPGEHNGTFRGINPAFVA
                     STEALKTYWTDDTLEKSTLAKGEQVGRALTDLALAYPEAALKAKGRGLARGLQFA"
     gene            5001..5402
                     /locus_tag="BLR56_RS30400"
                     /old_locus_tag="SAMN05421871_114107"
     CDS             5001..5402
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814642.1"
                     /locus_tag="BLR56_RS30400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421871_114107"
                     /product="ectoine synthase"
                     /protein_id="WP_091379684.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.5e-53, bitscore:
                     172.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSVHDLTDTERDVKTPTWRSKRIILAQEGVGFSVHETTLEPG
                     TVNDFWYANHVEAVFVVEGDGELLDKETGETHVLAPGSLYLLDGNERHQLRPKTRMRTV
                     CVFNPPVTGREVHDENGVYPLLTTEGEAR"
     gene            5399..6271
                     /gene="thpD"
                     /locus_tag="BLR56_RS30405"
                     /old_locus_tag="SAMN05421871_114108"
     CDS             5399..6271
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814641.1"
                     /locus_tag="BLR56_RS30405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421871_114108"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_091379681.1"
                     /sec_met_domain="PhyH (E-value: 1.4e-31, bitscore: 103.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLSTQDRYRTRTRDRLEPISRIEPTVWSGVDGPIDAETLVAHDA
                     RGFSVFQDLLGPSEVRAFQDELRRLSTDDKLKADERTIVESKSQEVRSIFEVHKISDLI
                     AELVRDPRVIERARQILGSEVYIHQSRVNYMPGFRGKGFYWHSDFETWHAEDGMPSPRA
                     VSMSIALTDNFPFNGGLMVMPGSQRTFIPCVGETPDQHYKASLSMQEIGVPSEADITTL
                     ANACGIEQFTGPAGSALMFDSNVMHGSGSNITPFPRSNIFVVFNSVENEVVEPFAAAQP
                     RPSFIAARR"
     misc_feature    complement(6287..6303)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.42"
                     /tool="antismash"
     gene            6566..9337
                     /locus_tag="BLR56_RS30410"
                     /old_locus_tag="SAMN05421871_114109"
     CDS             6566..9337
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /locus_tag="BLR56_RS30410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421871_114109"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_091379678.1"
                     /transl_table=11
                     /translation="MDSSDDDGPRFCVLGPVGVAVGDQLHSIGGPGLRGLLAILLLEPN
                     QVVPIERIVDVLWDHEPPVTARTIVQGYVSRTRKWLSTVDSTTSIETTGAGYQLVIDES
                     RIDVSMARRLLAGARGREPEVRSEMLARAQALWRGPELSDIGDRVRAPELAELRLAVIE
                     ARIDADLELGRHDDVIGELAALVDTHPFREHLVGQLVLALYRSGRRAGALEIYQRFAQR
                     AAGELGLDPGPGLRELHSRVLRDDARLLKPRAEPVVSPRLGVLNPAQLPAPPSGFAGRE
                     SELAWLDGVRGAGVAVLAGPAGIGKSALALLWGSQVAREFVHGQLYVSLRGFDPRHPPL
                     DAADVLTRFLLSLGVAAVDIPSDVAERAALYRSLLADRKVLVLLDDARDSEQVRPLLPG
                     AGRSLVLVTSRRRLDGLVADGARLRVVDTLAADAAVQLIEHAAGPPLNAAERAQRARLA
                     RLCGFLPLALRIAGARLAVSPQWTVGDLVAELSDERTRLGALDVEDANTSVRAALDVTV
                     RRLDEPHAATLRALGAFPGLWVSPYAVAALCGTDVGRARARLRVLAGSFLVSELDRDVY
                     GMHDLVRLFARELAGSGVESLRAVAGHYLYAADACRRHLRAVDDDITPAAAPCPDIPDR
                     EAALDWFDREWPNLIAVAAACADAGLHREVWQLARVAGDYRRVRSRRDDWEWLLDMGLT
                     SARACGEVRGQVLLHLSRCVLLSRFGAEHETTADADAAVMLAADLGEPKLSAMAHNTRA
                     SAWYGQKRFAEALGGYQEALSLARVAGYRLGEANLHNNIAQVHRNLGEPREALRPQRTA
                     VELYREVGDLGFMGLALANLAELEHELGLLTESAANAREAVALAEVNGLELTEAFAREV
                     LARTLRDSGDIAGARTELVIAIERYRQAQSPAADAARAALASLPPSEDPSRA"
     misc_feature    complement(9387..9397)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            9448..9957
                     /locus_tag="BLR56_RS30415"
                     /old_locus_tag="SAMN05421871_114110"
     CDS             9448..9957
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /locus_tag="BLR56_RS30415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAMN05421871_114110"
                     /product="pyridoxamine 5'-phosphate oxidase family protein"
                     /protein_id="WP_091379675.1"
                     /transl_table=11
                     /translation="MPLTNPWLAEPTPTGKLDREQLDERILNLLSSQNMCVLATAGPDG
                     PLATPVRYYPLGFAVLFTAARRSPKLRNIAADPRVSVGVFAPLVGAASSRGAQLFGTAR
                     VLDPDHPDRAHYWEAFRWENEHAERGRPLSEPPRDTLVVIEADRIVYTEHWLSREGFAP
                     RQFWRR"
     misc_feature    10159..10174
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.74"
                     /tool="antismash"
     misc_feature    10225..10240
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.05"
                     /tool="antismash"
ORIGIN
        1 tcccaatgag cctggaggcc gccatggacg acgatcggcg cgcgcttgcc tccgccaacg
       61 ggtccgaccc gaccgccgag atggtcgacc gggcgggtcg ggccctcggg tggctctatc
      121 gcagcgggtg gcaggtcgca cggcggctgc cgggcggcga ggtggccgag cggcaggtgc
      181 agaaggtcga ggaagcgatc gtggccgagg tgcgcaagca cctggagccg ctgcccgacc
      241 cggagccccg gcagatcgag gcggccaggg tccgggagcc gatgcggctg gcgatggcgc
      301 agctgctgga gcggtcggtc accgacagca aggacgccgc ccgcgaccac tacttcgagt
      361 ccgtcctgcg ccagctgctg cccgacgagg cgcggatcgt ctccgcgctg tccgacggca
      421 cggtcttccc gctgatgcac ctcgccgcgc ggaccgcgcg cggcggggcg ccgcggttcg
      481 tgctggagaa cgcgtcgacg gtcggccggg tcgccggggt ggtccagccg ggtctcgtcc
      541 cgctctacgt cggcaggctg ctgcggttcg ggctcgtcga ggtcggtgag gaggacccga
      601 cgctcggggt ggcctacgag atgctgctga ccgacgaccg ggtccgcgcc gccgaggacc
      661 gggcgaaggc gcagagccgg ttcggcgcga aggcgatccg gctgaccctg cggatctcgg
      721 agctgggcgc ccagttctgg gcggtctgcg acccgagtgc ccgcggcctg acgctcggcg
      781 agcgccgatg aacgcgatgc tgcaggactt ccagaccaac tggatggtct acgcgtcgat
      841 gccgctgatc gccgccctga tcggctacgt caccaagctc gtcgccatcc ggatgatgtt
      901 ccagccgctg gagttcgtcg gcaagaagcc atacctcggc tggcagggca tcgtgccgcg
      961 gcgggccgcc cggatggcca gcatcgccgt cgacacgatg accgaccggc tgatctcggc
     1021 gagcgaggtg atgggcaggc ttgacccgga gcgggtggtc aaggagatcg agcagccgct
     1081 gctcgccggg gtcgaggaga tcgccgccga gatcgcgcag gagttccagc cggggctgtg
     1141 ggagaacctg cccgagcagg tgcggagccg gatcgtgcgg cacatccagg ccgaggcgcc
     1201 gggtgtggtg gcctcgctgc tggagacggt caagaacgac gtcgacgccg tgttcgacct
     1261 caaggacatg gtcgtcacca acctggtgcg cgacaaggcg ctgctcaacc ggatcttcca
     1321 gcacgcgggc cgcaaggagt tccagttcat cgcccggtcc ggcatcgtgt tcggcttcgt
     1381 catcggcttc gtgcagatgt tcgcctgggc ggcgctgaag aacccgtggg tgatgccgat
     1441 cttcggcggc ctgaccgggt ggttcaccga ctggctggcc ctgaagatga tcttcaatcc
     1501 gaagaagccg acccgctacc tcgggatctt cgagtggcag ggcctgttcc tcaagcgccg
     1561 caaggaggtc gccgcggact acggcgacct gatcgcccgc gagatcatca ccccgaagaa
     1621 cgtcatggag gcggtcctgc gcgggccgct gtcggaccgg ctgttcgcgc tggtcgccgc
     1681 ccaggtccgg accgtgatcg acaagcagtc cggtccggcc cggccgctgg tgatgctggc
     1741 cgtgggcagc gggcgctacc aggcgatcaa gaacagcatc gcggagaagg tcatcgcccg
     1801 actgccggag acgatgaccc acatcgagtc ctacgccgag gacgcgatgg acatccgcaa
     1861 cacgctggtc accaagatgc aggagatgtc ggaggacgag ttcgaggagc tcatccggcc
     1921 cgcgttcgag caggacgagt ggatcctgat caccgtcggc gccgtcctcg gcttctgcgt
     1981 gggagaactg caggtcatcc tcgtcgagaa cctcgcccac tgaccccgcg ggggtgcggg
     2041 gtgtggcgca cgcaactacg gtcgggcgta tggcctatgt cgcgtcggac gaccggtatg
     2101 accgcatccc ctaccggcgg tgcggtcgca gcgggctgaa actgcccacc gtctccctcg
     2161 ggctctggca caacttcggc gacgaccagc cgcacgagat cgggcggacg atcctgcgcc
     2221 gcgcgttcga cctcggcgtc acccacttcg acctggccaa caactacggc ccgccctacg
     2281 gctcggcgga gcgcaacttc ggccgcgcgc tggagcgcga gttcggcgcc taccgcgacg
     2341 agctggtgat ctccacgaag gccggctacg acatgtggcc cggcccctac ggcgagtggg
     2401 ggtcgcgcaa gtacctgctg tcctcattgg accagtcgct caagcgcatg ggcctggact
     2461 acgtcgacat cttctactcc caccggttcg acccggacac gccgctggag gagaccgtcg
     2521 gcgcgctcgc gagcgccgtg cagcagggca aggccctcta cgtcggcgtc tcgtcctact
     2581 cgccgaccgc gacccggcag gcggccgagc tgctgcgcgc cgccggggtg ccgctgctca
     2641 tccaccagcc gtcgtactcg atgctcaacc gctggatcga ggacgagctg ctcgacgccc
     2701 tcggcgacct gggtgtgggc tgcatcgcgt tttccccgct ggcgcaagga ctgctgaccg
     2761 acaagtacct cgacggcgtc ccgtcgggct cgcgcggcag ccgggactcg tcgctgctgc
     2821 ccgagatgct gtcggaggac aacctgcggc gggtgcgtgg cctcaacgag atcgccaagt
     2881 cccgtgggca gacgctcgcc caactggcgg tgtcctgggt ggtgcgcgac gatcgggtca
     2941 cgtcggcgct ggtgggcgcc agctcggtcg agcagttgga gagcaccctg cgggcggtcg
     3001 acgggccgcc cttcaccgac gacgaattgg ccgagatcga ccggtacgcg gtggattccg
     3061 gcatcgacct gtgggaaagc tcccgatcat cgggctagtc cgttaacacg gtgtcagcgg
     3121 gccgcacaac cgtgatgttg tcctcctcac gggggtattc acgaaaccgc tggttaaccg
     3181 gtcgcgcgcc gatcggccat ccggtctgcc tgaaacgacc ctgttacgtt gccttggcat
     3241 gtcgggaaaa cgggtacgcg aggagatcgg cgcaccatcc atagcggatg gcgcggcgat
     3301 ctggagaatc gcgcgtgatt cacgcgcgct cgacctcaat tcgtcctatg cctatctgct
     3361 gtggtgccgg gatttcgccc agacctccgc tgtcgctcgc atcgacggcc aagtggtggg
     3421 tttcgtcatc ggattcttgc ggccggagtg cccggacacg gtcgtggtgt ggcagatcgc
     3481 cgtcgacgag tcgcagcgcg ggtcgggtct cggtggtcgg ctacttgatc acctgatggc
     3541 tcgcgtcgca gctcaggggg tgtgtcgtct ggagacgacg atcaccgcgg acaacgccgc
     3601 ctcgatcggc ctgttcgcct cgctggcgcg gcgccaccac gcgcgactgg ggcgcagcga
     3661 gttgttcagc gcggatcact tcccggatga ccacgaggct gaagaccttt accgaatcga
     3721 ctttccgaac ggggatggag agcagtgagc gttttcgaca tgatcgagtc cgaggttcgg
     3781 agctattgcc gcagctggcc ggtgacattc gaccgggcga ccggaagtcg catcttcgat
     3841 gagaatggcc agggctacct cgacttcttc gccggagccg gagcgctcaa ttacgggcac
     3901 aacaacccga ttctgaaaca ggccgtgatc gactacatcc agcgcgacgg cgtgacgcac
     3961 tcgctggaca tgcagaccgt cgccaagcgg gacttcctgg agaccttcag cgagacggtg
     4021 ctcgctcccc gcgagctcga ctacaagatc atgttcccgg gaccggcggg cgcgaacgcc
     4081 gtcgaggcgg cgctgaagct ggcccgcaag gtcaccggcc gcgagtcgat catcaacttc
     4141 accaacgcct tccacggcat gacgctgggc gcgctgtcgg tcaccggcaa ctcgctcaag
     4201 cgcggcggcg cgggcgtccc gctggtgcac gccaccccga tgccgttcga caactacctc
     4261 gacggcctca ccccggactt catgctgttc gaacggctgc tcaaggacag cggcagcggc
     4321 ctcaacgagc ccgccgcggt catcgtggag accgtgcagg gcgagggcgg gatcaacgtc
     4381 gccagcgtcg agtggctgcg cagcctctcg gagctgtgcc aacgccaggg catcctgctg
     4441 atcgtcgacg acgtgcagat gggctgtggc cggaccggtc cgttcttcag cttcgaggcc
     4501 gcgggcatca aacccgacat cgtctgcctg tcgaagtcca tcggcggcta cgggctgccg
     4561 ctgggcctga ccctgctgcg tcccgaactc gacgtgtggg agccgggcga gcacaacggc
     4621 accttccgcg ggatcaaccc ggcgttcgtc gcctccaccg aggccctgaa gacctactgg
     4681 accgacgaca cgctggagaa gtccacgctg gccaagggcg agcaggtcgg ccgcgcgctg
     4741 accgacctcg cgctggccta ccccgaggcc gccctcaagg ccaagggccg cggcctggcc
     4801 cgcggcctgc agttcgccga cggcgacatc gcgggcaagg tctgccgcgc cgcgttcgac
     4861 cgggggctgc tgatggagac ctccggcccc gacggcgagg tcgccaagat catgccgccg
     4921 ctcaccgtca ccgacgccga gttggaggaa ggcctggcga tcgtcgacga cgccgtcgcc
     4981 gcgaccctgg gggtcctgcg gtgatcgtcc gctcggtgca cgacctgacc gacaccgaac
     5041 gcgacgtcaa gaccccgacc tggcgcagca agcggatcat cctcgcgcag gagggtgtcg
     5101 gcttctccgt gcacgagacg acgctggaac cggggacggt caacgacttc tggtacgcca
     5161 accacgtcga ggccgtgttc gtcgtcgagg gcgacggcga gctgctcgac aaggagaccg
     5221 gcgagaccca cgtgctcgcg cccggatcgc tctacctgct cgacggcaac gagcgtcacc
     5281 agctccggcc gaagacccgc atgcgcacgg tctgcgtgtt caacccgccc gtgaccggcc
     5341 gcgaggtgca cgacgagaac ggcgtgtacc cgctgctgac gaccgaagga gaagcgcggt
     5401 gacgctctcg acccaggacc gctaccggac ccgcacccgc gaccggctcg agccgatctc
     5461 ccggatcgag ccgaccgtct ggtccggagt ggacggaccg atcgacgcgg agacgctggt
     5521 cgcccacgac gcgcgcggct tctcggtgtt ccaggacctg ctcggcccct ccgaggtgcg
     5581 cgcgttccag gacgagctgc ggcggctgtc cactgacgac aagctcaagg ccgacgagcg
     5641 cacgatcgtc gagtcgaagt cgcaggaggt ccgctcgatc ttcgaggtgc acaagatcag
     5701 cgacctgatc gccgaactgg tgcgcgaccc ccgggtgatc gagcgggcgc ggcagatcct
     5761 cggctccgag gtctacatcc accagagccg ggtcaactac atgccgggct tccgcggcaa
     5821 gggtttctac tggcactcgg acttcgagac ctggcacgcc gaggacggca tgccgagccc
     5881 gcgcgcggtg agcatgtcga tcgcgctgac cgacaacttc ccgttcaacg gcggcctgat
     5941 ggtcatgccc ggctcgcagc gcacgttcat cccgtgcgtc ggcgagacgc ccgaccagca
     6001 ctacaaggcg tcgctgtcga tgcaggagat cggcgtgccc agcgaagccg acatcaccac
     6061 cctcgccaac gcgtgcggca tcgagcagtt caccgggccc gccgggtcgg cgctgatgtt
     6121 cgactccaac gtcatgcacg gatcgggcag caacatcacg ccgttcccgc ggtccaacat
     6181 ctttgtcgtg ttcaacagtg tggagaacga agtggtggag ccgttcgccg cggctcagcc
     6241 gcggccgtcc ttcatcgcgg ccagaaggtg accgatcgct cgcggtttgt taagttgtcg
     6301 cgaaccgcga tgcggagatg cttcgtcgga tgttgaccaa gagcccgctg tgaccgcact
     6361 cggggtgcag tccggtgcgc tgtcggtcaa cttcgatgga gagtgcgccc ggggtcgtcc
     6421 gaatcgggaa cggacggacc ccgggcgtac gccaatcggc tgactcttcg agtaccccct
     6481 acgtcaccac ccgtttgccc ataggttgtc cagcggtgtg tgaacgcgcc cgtggggacg
     6541 ggcgcgtcaa ccggggggtg gcggggtgga ttcaagtgat gacgacgggc cgcggttctg
     6601 cgtgctcggc ccggtcggtg tggcggtggg ggaccagctg cactccatcg gcgggccggg
     6661 actgcggggg ctgctcgcga tcctgctctt ggaacccaat caggtggttc cgatcgagcg
     6721 gatcgtcgat gtgctgtggg accacgaacc gcccgtgacg gcccgcacga tcgtgcaggg
     6781 ctatgtgtcc cgcacgcgca agtggctgtc cacagtggac tcgacgacgt cgatcgagac
     6841 gaccggggcg ggctaccagc tggtgatcga cgagagccgg atcgacgtct cgatggcccg
     6901 ccggctgctc gcgggggccc gtgggcgtga gcccgaggtc cgctcggaga tgctcgcccg
     6961 ggcacaggcg ctgtggcgcg ggccggagct gtccgacatc ggcgaccggg tccgggcccc
     7021 ggaactcgcc gagctgcggc tcgcggtgat cgaggcccgc atcgacgccg acctcgaact
     7081 cggcaggcac gacgacgtga tcggcgaact agccgcgctg gtcgacaccc atccgttccg
     7141 ggagcacctc gtcgggcagc tggtcctggc gctctaccgg tcgggtaggc gcgcgggtgc
     7201 gctggagatc taccagcgct tcgctcagcg ggcggcgggc gagcttggcc tcgacccggg
     7261 ccctggcctg cgtgagctgc acagccgggt gctgcgcgac gacgcgcggc tgctcaagcc
     7321 acgggcggag ccagtggtca gcccgcggct cggggtgctg aacccggcgc agcttcccgc
     7381 gccgccgtca ggcttcgccg ggcgcgagtc ggagctggcg tggctcgacg gggtgcgcgg
     7441 cgcgggggtc gcggtgctcg ccggtccggc ggggatcggg aagagtgccc tggcgctgct
     7501 gtggggcagc caggtcgcgc gggagttcgt ccacgggcag ttgtacgtgt cgctgcgtgg
     7561 gttcgacccc aggcacccgc cgctcgacgc ggctgacgtg ctcacccggt tcctcctgtc
     7621 gctcggtgtg gccgcggtgg acatcccgtc cgacgtggcc gagcgggccg cgctctaccg
     7681 gtcgctgctg gccgaccgca aggtcctcgt gctgctcgac gacgcccgcg actccgagca
     7741 ggtccggccg ctgctgccgg gggccggacg gtcgttggtg ctggtcacga gcaggcgaag
     7801 actcgacgga ctggtcgccg acggcgcgcg gctgcgggtc gtggacaccc tcgccgccga
     7861 cgccgccgtg cagctgatcg agcacgcggc gggcccgccg ctcaacgcgg ccgaacgcgc
     7921 gcagcgggca cggctggccc ggctgtgcgg gttcctgccg ctggcgctgc ggatcgcggg
     7981 cgcacggctc gctgtcagtc cacagtggac tgtcggtgac ctggtggcgg agctgtccga
     8041 cgagcgcacc cggctgggcg ccctcgatgt ggaggacgcg aacaccagcg tccgggccgc
     8101 cctggacgtc acggtccgcc gtctcgacga gccgcacgcg gccaccctgc gggcgctggg
     8161 cgcgtttccg gggctgtggg tgagcccgta cgcggtggcc gcgctgtgcg ggacggatgt
     8221 ggggcgggcg cgggccaggc tgcgggtgct cgccgggtcg ttcctggtct cggaactgga
     8281 ccgcgacgtc tacggcatgc acgacctggt ccgcctcttc gcgcgcgaac tcgccgggtc
     8341 cggcgtggag tcgctgcggg cggtggccgg gcactacctc tacgccgccg acgcgtgccg
     8401 ccgacacctg cgcgcggtcg acgacgacat cacgcccgcc gcggcaccgt gtccggacat
     8461 ccccgaccgc gaagccgcgc tggactggtt cgaccgcgag tggcccaacc tgatcgcggt
     8521 cgccgcggcc tgcgccgacg cgggcctgca ccgggaggtc tggcagctgg cccgggtcgc
     8581 gggcgactac cgccgggtcc gctcccgccg cgacgactgg gaatggctgc tggacatggg
     8641 tctcacgtcg gcccgagcgt gcggggaggt gcgcggccag gtgctgctgc acctgagccg
     8701 ctgcgtcctg ctgagccgct tcggcgccga acacgagaca acggccgacg cggacgccgc
     8761 tgtgatgctc gctgcggacc tcggcgagcc caaactcagc gcgatggcgc acaacacgcg
     8821 ggccagcgcc tggtacggcc agaagcgctt cgcggaagcc ctcggcggct accaggaggc
     8881 cctgtcgctg gcgcgggtcg cggggtaccg gctgggggag gcgaacctgc acaacaacat
     8941 cgcccaggtc caccggaacc tgggcgagcc gcgggaggcg ctgcggcccc agcggacggc
     9001 ggtggagctg taccgggagg tcggggacct cggcttcatg ggcttggcac tggcgaacct
     9061 ggccgagctg gaacacgaac tgggcctgct gacggagtcc gccgccaacg cacgggaggc
     9121 ggtggcgttg gcggaggtca acggcctgga actcacggag gccttcgccc gcgaagtcct
     9181 ggcccgcacc ctgcgcgaca gcggcgacat agcgggcgcc cgcaccgaac tggtgatcgc
     9241 gatcgaacgc taccgccagg cccaatcccc agccgccgac gccgcccgcg cggccttggc
     9301 gtcccttccg cccagcgagg acccaagccg agcttgaacc ccgctgtacc agcgcaaacc
     9361 ccccactcag gggttgctct gtccgcgtga catgacatca ctcggcggca tctcggtagg
     9421 atccccggcc atgatcgagg tcgccgcgtg ccgctgacga acccctggct ggccgagccc
     9481 acaccgaccg gcaaactcga ccgcgaacag ctcgacgagc gcatcctcaa cctgctgtcg
     9541 tcacagaaca tgtgcgtgct cgcaaccgct gggccggacg ggccgctggc caccccggtg
     9601 cgttattacc cgctcggctt cgcggtgctg ttcactgccg cgcggcgctc gccgaagctg
     9661 cggaacatcg ccgccgaccc acgcgtgtcg gtcggtgtct tcgcgccatt ggtcggggcg
     9721 gcgagcagcc gtggcgctca actgttcggc accgcgcggg tgctcgaccc ggaccacccc
     9781 gaccgcgccc actactggga ggcgttcagg tgggagaacg agcacgcgga acgcggcagg
     9841 ccgttgtccg agccgccacg cgacacgctc gtggtcatcg aggcggatcg gatcgtctat
     9901 accgagcact ggctgagtcg tgagggcttc gcgccacgcc agttctggcg ccgttgagaa
     9961 cgcgttcgcc cgtcttggcg gacaaccagc cccgcgttta gcgaggattt agcgggcctg
    10021 atcgagtcgc cggcagggtt gcccgcggcg cccagtcagg tgccattggg ctgcgcggct
    10081 tgggggtgcg cggcccggac gtaccggccg gcgctcctgg ggggagagag ccggccggtc
    10141 gtttgtccgg gtaagggtct aggtccagtc ctgtaagtcc ggcacacggt attcgcaccg
    10201 gacttacagg acaggaccta gacgttggcc gcgtggtcgt cgccccggta cgggcgggac
    10261 tcgcggtagg ggcgcagttc ccggtgcttc ggcgcgaggc cgtcgcccat cgactcgccc
    10321 atgatctgcc tgcggatcca cgggatcagg tgcgtcgtgg tccacgcgag gtcggcctgc
    10381 cgcgagtgca gccacggctt gg
//
