LOCUS       NZ_FMCU01000001        10819 bp    DNA     linear   CON 02-MAY-2024
DEFINITION  Micromonospora matsumotoense strain DSM 44100, whole genome shotgun
            sequence.
ACCESSION   NZ_FMCU01000001
VERSION     NZ_FMCU01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Micromonospora matsumotoense
  ORGANISM  Micromonospora matsumotoense
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Micromonospora.
REFERENCE   1
  AUTHORS   Varghese,Neha. and Submissions,Spin.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2016) DOE - JOINT GENOME INSTITUTE, 2800 Mitchell
            Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900091525.1-RS_2024_05_02
            Annotation Date                   :: 05/02/2024 03:45:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,745
            CDSs (total)                      :: 6,674
            Genes (coding)                    :: 6,498
            CDSs (with protein)               :: 6,498
            Genes (RNA)                       :: 71
            rRNAs                             :: 5, 3, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 176
            CDSs (without protein)            :: 176
            Pseudo Genes (ambiguous residues) :: 0 of 176
            Pseudo Genes (frameshifted)       :: 35 of 176
            Pseudo Genes (incomplete)         :: 151 of 176
            Pseudo Genes (internal stop)      :: 7 of 176
            Pseudo Genes (multiple problems)  :: 16 of 176
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 09:03:39
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 177373
            Orig. end                         :: 188192
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FMCU01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10819
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10819
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10819
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5819](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5819
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1105..1353
                     /locus_tag="GA0070216_RS00730"
                     /old_locus_tag="GA0070216_101147"
     CDS             1105..1353
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287422.1"
                     /locus_tag="GA0070216_RS00730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070216_101147"
                     /product="DUF2630 family protein"
                     /protein_id="WP_091237304.1"
                     /transl_table=11
                     /translation="MDDKTILHRITELVDEEHRLRSTAQAHESGTDDEAKEQLRALEES
                     LDQCWDLLRRRRAAREAHGDPEAQGARPVSEVERYLQ"
     gene            complement(1402..2331)
                     /locus_tag="GA0070216_RS00735"
                     /old_locus_tag="GA0070216_101148"
     CDS             complement(1402..2331)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018583044.1"
                     /locus_tag="GA0070216_RS00735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070216_101148"
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="WP_091237305.1"
                     /transl_table=11
                     /translation="MRDHDLPAPQRRGRGKSRFDDDETPFLTYGRQPARTAPDDEPEPG
                     PEDHWSSWDEAVHGPEPHPPWLVTELAARDTELGVLKTGKEADVHLVRRAVPDTDRSCL
                     LAVKRYRDAQHRLFHRDAGYLEGRRVRRSRENRAMAGRTAFGRQMIAGQWAAAEFAALS
                     RLWEIGADSGRIAVPYPVQLRGTELMLEFVGDPDEGTAAPRLAQVRPDPAPLRSLWEQL
                     VDALVVLARAGYAHGDLSPYNLLVHAGRLVMIDLPQVVDVVANPQGPEFLARDVRVVAA
                     WFTARGLPAADTDPAALTAMLLREAGVR"
     gene            complement(2489..3136)
                     /locus_tag="GA0070216_RS00740"
                     /old_locus_tag="GA0070216_101149"
     CDS             complement(2489..3136)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287424.1"
                     /locus_tag="GA0070216_RS00740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070216_101149"
                     /product="HAD-IA family hydrolase"
                     /protein_id="WP_091237306.1"
                     /sec_met_domain="HAD_2 (E-value: 2.3e-12, bitscore: 42.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPLLLLDLDNTLLDRAGPFRAWAEGFLAEIGAPATDIDWLLSIDA
                     DGLTDRWDIADAIRDRYALRIPSIDLVEELHDGVVGYTRLDPLVACALRIADDAGWLPV
                     VVTNGSVRLEDTKIRQTGLDRYIADWVISEEAGVSKPNPRIFALAAQRARLPLRGAWVV
                     GDSPEADIGGAAAVGLPSVWLHRGRSWTDGRFAPTRTVDSLIAAVSVVLAGG"
     misc_feature    complement(3338..3351)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 18.61"
                     /tool="antismash"
     gene            3368..3940
                     /locus_tag="GA0070216_RS00745"
                     /old_locus_tag="GA0070216_101150"
     CDS             3368..3940
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018727348.1"
                     /locus_tag="GA0070216_RS00745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070216_101150"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_091237307.1"
                     /transl_table=11
                     /translation="MPRVSQDQLDARRQEILGAARACFARHGYEGATVRRLEEATGLSR
                     GAIFHHFRDKDSLFLAVAEDDAAVMVSTVARNGLVQVMRDLLARAVSPDTTGWLGSQLE
                     VSRRLRTDPAFARRWAERSAAIAEATRDRLVRQREAGVLREDIDIDVLAQFLELAYDGL
                     VLHLAMGRPAGDLGPVLDLVEEAVRRR"
     gene            4060..5004
                     /locus_tag="GA0070216_RS00750"
                     /old_locus_tag="GA0070216_101151"
     CDS             4060..5004
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183391.1"
                     /locus_tag="GA0070216_RS00750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070216_101151"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_091237308.1"
                     /transl_table=11
                     /translation="MNTLAAPGDTWGIPGPLFLKLYLLVAVVAVVGTLLHRRRATAGPD
                     PVTADQLGPQQVAYLNGGDQLAIWAALGGLRAAGAIDVRPDRRLIAAGPTPTGVTPLDH
                     AIRHAARQPVAAQKLGRDSGVARALEQLRQGLEQRGLLIDADGVRALRRGRNLLLVLLA
                     LGVVRLVAGLVNARPVGWLLVALLVLGVALALLARTPRRTRTANVLLRRLRQSSHHLSP
                     DSAPAYATYGAAGAAMGVALFGTASLYALDPGFAAQAEIQRQALNASSGSSYSGSSGTS
                     CGGSSSSSSCSGGSSSSCGGGGSSCGGGGGCGG"
     gene            5001..5819
                     /locus_tag="GA0070216_RS00755"
                     /old_locus_tag="GA0070216_101152"
     CDS             5001..5819
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018802926.1"
                     /locus_tag="GA0070216_RS00755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070216_101152"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_091238766.1"
                     /sec_met_domain="DUF692 (E-value: 1.2e-84, bitscore: 279.2,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTWPHGVGIGWRPEIAGFVAELPGLRFVEVVAESLAAGGPLPAGL
                     AALRERGVTVVPHGVRLSLGGAEPVDPHRVAHLARVAELVDAPLVSEHVAFVRAGGLEA
                     GHLLPLPRTREAVTAVVANVRRAQAELPVPLALEPIAALFDWPDDELDEADFLTEILDA
                     TGAGLLLDVANVYANARNRGDDPHALLDRLPLERISYVHVAGGAERDGLYHDTHTDPVP
                     AEVLELVGALCARRPPPALLLERDGHYPPAAALRAELDALAVAAGHPVVT"
     gene            5816..6292
                     /locus_tag="GA0070216_RS00760"
                     /old_locus_tag="GA0070216_101153"
     CDS             5816..6292
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018223393.1"
                     /locus_tag="GA0070216_RS00760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070216_101153"
                     /product="hypothetical protein"
                     /protein_id="WP_091237309.1"
                     /transl_table=11
                     /translation="MSTPHPAGPTPPTGLAARQAALVATLVAGAPVPAGFVTDRVDAAR
                     AALLRKRAGEVARHWPLLAAGLGDAWWGTFRDWAAGRPTAGSLRDGWDLARELRDRGAL
                     PPLGAEELVVREAAHHYDGEQPPRHRRLPAFARAGDAVALQFAGRVRLLRPARR"
     gene            6370..7122
                     /locus_tag="GA0070216_RS00765"
                     /old_locus_tag="GA0070216_101154"
     CDS             6370..7122
                     /NRPS_PKS="Domain: PKS_KR (8-149). E-value: 4.2e-07. Score:
                     22.0. Matches aSDomain:
                     nrpspksdomains_GA0070216_RS00765_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018815297.1"
                     /locus_tag="GA0070216_RS00765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070216_101154"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_091238770.1"
                     /transl_table=11
                     /translation="MDLGLTDRVYVLTGASRGLGFATARCLVADGARVVLSARTPERVA
                     EAVDRLGGPDHAIGLVADLADASLPERLVIAAGEHFGRLDGALVSVGGPPPGTAAQVSD
                     AQWRESFETVFLGTVRTARTVAAALTDGGAIAMVLSTSARTPITGLGISNGLRPGLAGV
                     AKDLADEYGPRGVRVLGLLPGRFLTDRNRELLGDDEAARSQAEAGIPLRRIGDPAEFGR
                     VAAFLLSPAAGYVTGVSVPVDGGALRSL"
     CDS_motif       6394..6486
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_GA0070216_RS00765_0001"
                     /evalue="7.20E-07"
                     /label="PKSI-KR_m1"
                     /locus_tag="GA0070216_RS00765"
                     /protein_end="39"
                     /protein_start="8"
                     /score="21.9"
                     /tool="antismash"
                     /translation="VYVLTGASRGLGFATARCLVADGARVVLSAR"
     aSDomain        6394..6816
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_GA0070216_RS00765_PKS_KR.1"
                     /evalue="4.20E-07"
                     /label="GA0070216_RS00765_PKS_KR.1"
                     /locus_tag="GA0070216_RS00765"
                     /protein_end="149"
                     /protein_start="8"
                     /score="22.0"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VYVLTGASRGLGFATARCLVADGARVVLSARTPERVAEAVDRLGG
                     PDHAIGLVADLADASLPERLVIAAGEHFGRLDGALVSVGGPPPGTAAQVSDAQWRESFE
                     TVFLGTVRTARTVAAALTDGGAIAMVLSTSARTPITG"
     gene            7284..7892
                     /gene="mug"
                     /locus_tag="GA0070216_RS00770"
                     /old_locus_tag="GA0070216_101155"
     CDS             7284..7892
                     /EC_number="3.2.2.28"
                     /GO_function="GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA]"
                     /GO_process="GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /gene="mug"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287431.1"
                     /locus_tag="GA0070216_RS00770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070216_101155"
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="WP_245722275.1"
                     /transl_table=11
                     /translation="MEVSPGPTRPREPEFRRPDRAELAAAADRTIPDVIDQGLTVLFVG
                     INPGLWSAATGWHFARPGNRFWPALHRGGFTPRQLHPSEQHTLPGYGLGITNMVARASA
                     RADELTPAELVTGARTLTDKVALYRPTWVAVVGVTAYRIGFARPKAGFGRQPEPLAGAR
                     LWVLPNPSGLNAHFTPQTLGAAFGALRAAVVGDPPPAVG"
     misc_feature    complement(7932..7950)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 16.75"
                     /tool="antismash"
     misc_feature    7939..7952
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            7956..8336
                     /locus_tag="GA0070216_RS00775"
                     /old_locus_tag="GA0070216_101156"
     CDS             7956..8336
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GA0070216_RS00775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GA0070216_101156"
                     /product="hypothetical protein"
                     /protein_id="WP_091237310.1"
                     /transl_table=11
                     /translation="MDSGLEAAINEWHASVNEGDLDRAARAVGDPVVVLGPRGAGTITP
                     AQFAEWVGRSGITLRPRSWHPVSERLMVVEEDATWPEHTEPTRVATVFRASGGKVTAAL
                     RLPDLKAALELAHICREMAASE"
     gene            8333..8770
                     /locus_tag="GA0070216_RS00780"
                     /old_locus_tag="GA0070216_101157"
     CDS             8333..8770
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /locus_tag="GA0070216_RS00780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070216_101157"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_091237311.1"
                     /transl_table=11
                     /translation="MTSQGPGQVLFHFVRHWARRPVAADAGGDQGRLVLVCEAVQALSQ
                     RGIPATVNAIAHDIGIDQSGASRLIKSATAAGYLAATASPADGRRRETSLTPEGRSMLD
                     RAHRWQEDIFAQLTTDWSDTKRRDFQQAMTDLMDRSYATGA"
     misc_feature    9369..9383
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     misc_feature    complement(10244..10257)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
ORIGIN
        1 ccgatggtcg actgccactc ctcggcggtc aacggcaggg tggtcgccga ccgggactgc
       61 tggatcaccc ggtcctccag cacccggaac cgggccaccg cgtccgagcc ggcgagctgg
      121 tcgtaccgga tccggtcggc cggtgacagc tcggcgaccg cctccgccgt ctggtagcgc
      181 tgggcgccga ccagttgggt gaaccgggcg tactcgtcgc cggtcatccg gcccgccgcg
      241 agcacgccgg tgatcagggc gtcctgttgg gagatcagct cccgcacctg gtagaggtcg
      301 accagggttt cactcgccgc ggcgatctgg gtgtcgtcga gctggccgag cgagccgtac
      361 atccgcagca ccgaggcgag gatcgcggcg taggtggtgg acgcctcggc ccggttcagc
      421 ccggcgtccc gcaccgactg ccggatgccg gtcagaccac tcaggccggc cagcacctcg
      481 tccacccgac gccgggtgtc ctcgccggtg gccagttcgg cctgccaact acccaccgac
      541 gtgccgaatt cgtccgccac ctggtcggtg cgctgccact gggtggtcaa cgcgtcgagt
      601 tgccgctggc cccgctcgcc caggtaggcg ttggcgagcc ggcgctcctc ctggagggcg
      661 gtgagcagcg actcccccgg gtcggccacc cggctgttga gggtctgtac ccagagcagg
      721 ttgaagccgt cacggacggt cacccaggcg gcgaaggacc agagcgcgac gagggaggtg
      781 agcagggcga tgattttggt acgcagacgc gtactgccgg aacccatcac accgcccccg
      841 ggccggaccg tggccaccgg acgcccgacg cgtcacggca cacctgaacc cggcgcacgc
      901 tagcagtcgg cagcaccatc gctcaagcca tgctgatcat ggaggcggga ccggtgccgc
      961 aggtccgggg gcgattgtga ccaccatgga tggtgccggt gcggcagccg cgacacccac
     1021 ccggtgcggg tcgcggccgg ccgacgccgg gccggccggt ccgaacgcgg gcgcgggcga
     1081 tcctcctgtg ccaggctcag gggcatggac gacaagacca tcctgcaccg gatcacggaa
     1141 ctggtcgacg aggagcaccg gctccggtcg acggcccagg cccacgaatc cggcaccgac
     1201 gacgaggcca aggaacaact gcgcgcgctg gaggagtccc tggaccagtg ctgggacctg
     1261 ctgcgtcgcc gtcgggcggc ccgggaggcc cacggcgacc cggaggcaca gggtgcccga
     1321 ccggtgtcgg aggtcgagcg ctacctccag tgacgccccc gaggtcccgg acggccgacg
     1381 gccgtccggg acgtcaccgg ctcagcgcac ccccgcctcg cgcagcagca tcgcggtgag
     1441 ggcggccggg tcggtgtcgg cggcgggcag cccccgggcg gtgaaccagg cggccaccac
     1501 ccgcacgtcg cgggccagga actccggccc ctgcgggttg gccaccacgt cgaccacctg
     1561 cggaaggtcg atcatgacga gccgcccggc gtgcaccagc aggttgtacg gcgacaggtc
     1621 gccgtgggcg tagccggccc gggccagcac caccagggcg tccaccaact gctcccagag
     1681 tgagcgcagc ggggccgggt cggggcgtac ctgggccagc cggggcgcgg cggtcccctc
     1741 gtcggggtcg ccgacgaact ccagcatcag ctcggtgccc cgcaactgga ccgggtacgg
     1801 cacggcgatc cgcccggagt cggcaccgat ctcccagagc cgggacagcg cggcgaattc
     1861 ggccgccgcc cactgaccgg cgatcatctg ccggccgaac gccgtccggc cggccatcgc
     1921 ccggttctcc cgggaccggc gcacccggcg tccctccagg tagccggcgt cgcggtggaa
     1981 gagccggtgc tgggcgtccc ggtaccgttt gacggccaac aggcaggacc ggtcggtgtc
     2041 gggcaccgcc cggcggacga ggtggacgtc cgcctccttg ccggtcttga gcacaccgag
     2101 ttcggtgtcc cgggcggcca gttcggtgac cagccacggt gggtgcggtt cgggcccgtg
     2161 cacggcctcg tcccaggagg accagtggtc ctcgggcccg ggctcgggtt cgtcgtcggg
     2221 tgcggtacgg gccggctgcc gcccgtaggt caggaagggt gtctcgtcgt cgtcgaagcg
     2281 gctcttgccg cggccacggc gctgcggcgc cgggaggtcg tgatcgcgca cggtgctggt
     2341 gatcccttgg tcgaggctgg tgagggcagg tggtacgacc ctgcaaagac ggccatgacc
     2401 gacctcctct cctcgaccgg gccccgcccg gctgcgcatt cctgcgcggg cccatgctgg
     2461 ccgacgcccc gaccggggca caaccgattt atccgccggc gagcaccacc gagaccgccg
     2521 cgatcagcga gtcgacggta cgggtggggg cgaaccggcc gtcggtccag ctgcggcccc
     2581 ggtgcagcca gacgctgggc aggccgaccg cggcggcccc gccgatgtcc gcctccggac
     2641 tgtcccccac cacccacgcg ccgcgcagcg gcaaccgggc ccgttgggcg gccagcgcga
     2701 agatccgggg gttgggcttg ctgaccccgg cctcctcgga gatcacccag tcggcgatgt
     2761 aacggtccag gccggtctgt ctgatcttgg tgtcctccag ccggaccgag ccgttggtga
     2821 ccaccaccgg cagccacccg gcgtcatcgg cgatccgcag cgcgcaggcc accaacgggt
     2881 cgagccgggt gtacccgacc accccgtcgt gcagctcctc gaccaggtcg atggagggga
     2941 tgcgcagcgc gtaccggtcc cggatggcgt cggcgatgtc ccaccggtcg gtcagcccgt
     3001 cggcgtcgat ggacagcagc cagtcgatgt cggtcgccgg cgcgccgatc tcggccagga
     3061 aaccctcggc ccaggcgcgg aacggcccgg cccgatcgag cagggtgttg tccaggtcga
     3121 gcaggagcaa cggcactgag gcaccttacg ggagagagga ccgccgccga cagggtcgaa
     3181 ccgcccgccg acggccgtcg ggctgccgac ggggggcggg ggcggtgggc ggatagcggg
     3241 aggtgaccgg ggctgtcggc accggccgat cctaaccgga cgggcgttcg gcaggccgag
     3301 gtgacgaccc aatagcaagc cgtacgtacg gtttgccttg cgcagggtca cctgcgacga
     3361 tcaacccgtg cccagagtaa gtcaggacca gctcgacgcg cgccgccagg agatcctcgg
     3421 tgcggcccgg gcctgtttcg cccggcacgg ctacgagggc gccaccgtcc gccgactgga
     3481 ggaggcgacc ggtttgtccc ggggcgcgat cttccatcac ttccgcgaca aggactcgct
     3541 cttcctcgcc gtcgccgagg acgacgccgc cgtcatggtc tccacggtgg cccgcaacgg
     3601 cctggtccag gtgatgcggg acctgctggc ccgggcggtc tccccggaca ccaccggctg
     3661 gctgggcagc caactggagg tgtcccgccg gctgcgtacc gacccggcct tcgcccgccg
     3721 gtgggcggaa cggtccgccg ccatcgccga ggcgacccgg gaccggctcg tccgacagcg
     3781 cgaggccggg gtgctccgcg aggacatcga catcgacgtg ctggcccagt tcctggagct
     3841 ggcgtacgac gggctggtgc tgcacctggc gatgggccgg cccgccggtg acctggggcc
     3901 ggtgctcgac ctggtcgagg aggccgtccg ccgacgctga cccggcccgt gccaggggca
     3961 gcgcgggtct cggctcggta actcgcccac cgccgccgtg ccggcgtggc ggggctgccc
     4021 cacaatgggg ccgcgatccc ctcgcgacag gagcagctca tgaacacgct tgccgcaccg
     4081 ggcgacacct ggggcatccc cgggccgctc ttcctgaagc tgtacctgct ggtggccgtc
     4141 gtcgccgtgg tcggcacgct gctgcaccgg cgtcgggcaa ccgccggccc cgacccggtg
     4201 accgccgacc agctcgggcc gcagcaggtg gcctacctca acggcggtga ccagctcgcc
     4261 atctgggcgg cgctgggtgg cctgcgcgcg gccggcgcga tcgacgttcg accggaccgc
     4321 cggttgatcg ccgccgggcc gacccccacc ggcgtcaccc cgctcgacca cgcgatccgg
     4381 cacgccgccc gccagccggt cgccgcccag aagctggggc gcgactccgg ggtggcccgg
     4441 gccctggagc agctgcgcca ggggctggaa cagcgcggcc tgctgatcga cgccgacggg
     4501 gtccgggcgc tgcgtcgagg acggaacctg ctgctggtgc tcctggccct gggggtggta
     4561 cggctggtcg ccggcctggt caacgcacga cccgtcggct ggctgctggt cgccctgctc
     4621 gtgctcgggg tggcgctcgc tctgctggcc cgtacgcccc gccggaccag gacggcgaac
     4681 gtactgctgc gccgcctgcg ccagagcagc caccacctct cccccgactc cgccccggcc
     4741 tacgcgacct acggcgcggc cggcgcggcg atgggcgtgg ccctgttcgg caccgcctcc
     4801 ctctacgcgc tcgaccccgg cttcgccgcg caggcggaga tccagcggca ggcgttgaac
     4861 gccagcagcg gcagcagcta ctcgggttcc tcgggcacct cgtgcggcgg tagcagcagc
     4921 agctcgtcgt gcagtggcgg ttcgtcctcc tcctgcggcg gcggcggcag ctcctgcggc
     4981 ggcggcgggg gatgcggcgg atgacctggc cgcacggggt gggcatcggc tggcggccgg
     5041 agatcgccgg tttcgtcgcc gagctgcccg ggctgcgctt cgtcgaggtg gtggcggagt
     5101 cgctcgctgc gggcggcccc ctcccggccg ggctcgccgc gctgcgggag cgcggcgtga
     5161 ccgtcgtacc gcacggggtg cggctctccc tcggcggcgc ggaaccggtc gacccgcacc
     5221 gggtggccca cctggcccgg gtggccgagc tggtcgacgc gccgctggtc agcgagcacg
     5281 tcgcctttgt ccgggccggc ggcctggaag ccggtcacct gctgccgctg ccgcgcaccc
     5341 gggaggcggt gacggcggtg gtggcgaacg tccgacgggc ccaggcggag ctgccggtgc
     5401 cgctcgcgtt ggagccgatc gccgcgctgt tcgactggcc cgacgacgag ttggacgagg
     5461 cggacttcct caccgagatc ctcgacgcca ccggggcggg gctgctgctc gacgtggcca
     5521 acgtctatgc caacgcccgc aaccggggtg acgacccgca cgccctgctc gaccggctgc
     5581 cgctggagcg gatctcctac gtgcacgtgg ccggcggggc cgagcgcgac gggctctacc
     5641 acgacacgca caccgatccg gtgcccgccg aggtgctgga gctggtcggc gcgctctgcg
     5701 cccgccggcc cccgcccgcg ctgctgctgg agcgcgacgg ccactacccg cccgccgccg
     5761 cgctgcgcgc cgagctggac gccctcgccg tcgccgccgg tcacccggtc gtcacatgag
     5821 cacgccccac ccagccggcc ccaccccgcc gacggggttg gccgcccggc aggccgcgtt
     5881 ggtcgcgacc ctggtcgcgg gagcgccggt gccggccggc ttcgtcaccg accgggtcga
     5941 cgccgcccgg gcggccctgc tgcgcaagcg ggccggggag gtcgcccggc actggccgtt
     6001 gctcgccgcc ggcctcggcg acgcctggtg gggcaccttc cgcgactggg cggccggccg
     6061 acccaccgcc ggttcgctgc gcgacggctg ggacctcgcc cgcgagctgc gggaccgggg
     6121 ggcgctcccc ccgctcggcg cggaggagct ggtcgtccgg gaggccgccc accactacga
     6181 cggagaacag ccgccacgcc accgccggct gccggcgttc gcccgcgccg gcgacgccgt
     6241 cgccctccag ttcgccggtc gggtacgcct gctgcgcccg gcccgccgct gaccccacgc
     6301 cccggaccgt ccggcccggg gcaccggccc cccgtgccga ccaccaccgg atacggcagg
     6361 atcggtggca tggatctcgg actcaccgat cgggtgtacg tgttgaccgg cgcttcccgg
     6421 gggctcggct tcgcgaccgc gcggtgcctg gtcgccgacg gggcgcgggt ggtcctgtcg
     6481 gcccgtacgc cggagcgggt cgccgaggcc gtcgaccggc tgggtgggcc cgaccacgcg
     6541 atcggcctcg tcgccgacct ggccgacgcg agccttcccg agcggctggt catcgccgcc
     6601 ggtgaacact tcggccggct cgacggagcg ctcgtctcgg tcggcgggcc gcccccgggc
     6661 accgccgcgc aggtcagcga cgcgcagtgg cgggagtcct tcgagacggt cttcctcggt
     6721 acggtccgga cggcccggac ggtggccgcc gcgctcaccg acggcggcgc gatcgccatg
     6781 gtgctgtcca cctcggcccg gacgccgatc accggcctcg gcatctccaa cggcctgcgg
     6841 cccggcctgg ccggggtggc caaggacctg gccgacgagt acggcccccg cggcgtacgg
     6901 gtgctgggac tgctgcccgg acggttcctg accgatcgca accgggaact gctcggcgac
     6961 gacgaggcgg cccggtccca ggcggaggcc ggcatcccgc tgcgccgcat cggcgatccg
     7021 gccgagttcg ggcgggtggc cgccttcctg ctctcccccg ccgccggcta tgtcaccggg
     7081 gtcagcgtgc cggtcgacgg cggcgcgctg cgcagcctgt gaccccggca cgccgcgcga
     7141 ccggccccgc cgtccccggc cccgccgccg tccctgccac caccggcccc gaaccggccc
     7201 gccgccgtcg gggcaccgac ggcgggcact cgccggcccc cgccgaccgc ctgcccggga
     7261 ccgtcgacgg gccacccggc cggatcgagg tctcccccgg cccgacccgc ccgcgcgagc
     7321 cggagttccg gcgaccggat cgggcggagt tggccgcagc ggcggaccgc accatccccg
     7381 acgtgatcga ccaggggctc acggtgctct tcgtcgggat caaccccggt ctctggtcgg
     7441 cggccaccgg gtggcacttc gcccggcccg gcaaccgctt ctggccggcc ctgcaccggg
     7501 gcggcttcac cccgcgccaa ctgcacccga gcgaacagca cacgctgccc gggtacgggc
     7561 tgggcatcac caacatggtg gcgcgggcca gcgcgcgggc ggacgaactg accccggcgg
     7621 agctggtgac gggcgcacgc acgctgaccg acaaggtggc cctctatcgg ccgacctggg
     7681 tggccgtggt cggggtgacg gcgtaccgga tcgggttcgc ccggccgaag gccggcttcg
     7741 gccggcagcc ggaaccgttg gccggcgcgc ggctgtgggt gctgcccaac ccgagcggcc
     7801 tgaacgccca cttcaccccg cagaccctgg gggccgcctt cggcgcactg cgcgcggccg
     7861 ttgtcggcga cccgcccccg gccgtcggct gaccgccacc cggtgcgggt cgccaccgag
     7921 cggccaatgt atgcgtatcg catatacttt caggtatgga ttcgggtctt gaagcggcaa
     7981 tcaacgagtg gcacgcgagc gtcaacgagg gcgacctgga ccgggccgcg agggcggtcg
     8041 gtgatccggt cgtcgtcctg ggcccgaggg gcgcgggcac gatcacgccc gcgcagttcg
     8101 cggagtgggt ggggcgttca gggatcacac tgcgccctcg gtcctggcac ccggtcagcg
     8161 aacgactcat ggtggtcgag gaggacgcca cctggcccga gcacacggag cccacacggg
     8221 tggcgacggt cttccgcgcc tccggcggga aggtcacggc tgcgctgcgg ctgccggatc
     8281 tcaaggccgc cctcgaactg gcccacatct gtcgcgagat ggcggcatcc gagtgaccag
     8341 ccaaggcccc gggcaggtcc tgttccactt tgtccggcac tgggcgcgcc gacccgtggc
     8401 ggcggacgcg ggcggcgacc agggccgact cgtgctggtc tgtgaggcgg tccaggcgct
     8461 cagccagcga ggaatcccgg caaccgtcaa cgcgatcgcg cacgatatcg ggatcgacca
     8521 gagtggcgcc tcccggttga tcaagagcgc cacggcagcc ggctacctgg cggcgacggc
     8581 gtctccggcc gacggtcgac gacgcgagac atccctcacc cccgagggcc ggtccatgct
     8641 ggaccgggca catcgctggc aggaggacat cttcgctcag ctgaccacag actggagcga
     8701 cacgaagcgc cgggacttcc agcaggcgat gaccgacctg atggaccgct cctacgcgac
     8761 gggggcatga gtcacgccaa cgcgtcatcg agcatcgaag ggcccggctc ggacaggctc
     8821 aggtccatcg gccccggcgc agacggcaac cgcaagcccg ggacgatcgc cgcccgctgg
     8881 cagcacccgc atccaccgct agttggccac ggccgggggc agcgcctcct gcgcgacgta
     8941 ggtgcccatc ggcacggtgg tgatgatcgg ctcgggctgg tccacctcgg cgtacggggc
     9001 gggcgagtcg acgaccgcga actgggtgcg gtacagctcg gcgtagagcc caccgaccgc
     9061 gaccaactcg tcgtgccggc cccgctcgac gatccggcca ccgtcgagga ccaggacctg
     9121 gtcggcgtcg cgcacagtgg agagccggtg cgcgatcacc agcgcggtac gcccggccaa
     9181 cgccacggac agggcccgct gcaccgccgc ctcgctctcc gagtcgagat gggcggtggc
     9241 ctcgtccagg atcacgatcg acggggcctt gagcagcagc cgggcgatgg cgatgcgttg
     9301 cttctcgccg ccggagaagc ggtagccgcg ctcgccgacc gtcgtctcca gcccgtcggg
     9361 cagggcccgg accaggtcgg ccacctgcgc gccggccagc gccgcccaga tctcgtcgtc
     9421 ggtggcgtcc ggcttggcgt accgcaggtt ctcggcgatc gtctcgtgga acaggtggga
     9481 gtcctgggtg accacaccga tctcgtcgcg cagcgaggcg agggtggcgt cccggacgtc
     9541 gaccccgccg acccgcaccg cgccgtcggt gacgtcgtag atccgggaga tcagcatgga
     9601 cagcgtggac ttgccggccc cggacggccc gaccagggcc accatctgcc ccggctcgac
     9661 gcggaacgag acgcccttga gcaccggctc gttggcggtc cggtcgaggg tggcgacctc
     9721 ctccagcgag gccagcgaga cctcggctgc cgccgggtag cggaaccgga cgtcgcggaa
     9781 ctcgacccgg ccggcacccc ggggcaccgg caccgcatcg ggcttctcct cgatgccggg
     9841 gcgcaggtcc agcacctcga agacccggtc gaaggagacc agcgcgctca tcacgtcgac
     9901 ccggacgttg gacagcgcgg tcagcgggcc gtagaggcgg gtgagcagca gggcgagggt
     9961 gaccaccgtg ccggcgctga ccgcaccggt gaccgccagc cagccgccca gcccgtaggt
    10021 gagcgcctgg gccagcgagg cgaccagcag catcgccacg aagaaggtcc gcgagtacat
    10081 cgccgactgg atgccgatgt cgcggacccg ctcggcccgc gcgccgaacc ggcccgcctc
    10141 cacctcgggc gagccgaaca gcttgaccag taacgcgccg gccaccccga accgctcggt
    10201 catggtggcg ttcatcttgg cgtcgaggtc gtacgactcc cgggtgatct cggccaaccg
    10261 cctgccgacc cgccgcgcgg ggaccacgaa gatcggcagc aggaccagcg acagcagggt
    10321 gatctgccag gagagggtga acatcacccc ggcggtgagc accaactgga tgacgttgct
    10381 gaccaccccg gacagggtgg acgtgaaggc ccgctgggcg cccatcacgt cgttgttgag
    10441 ccggctgacc agggcgccgg tctgggtccg ggtgaagaac tgcaacggca tccgctgcac
    10501 gtggtcatag acccgggtgc gcaggtcgag gatgatcccc tcaccgatac gggccgagta
    10561 ccaccgctgg gcgagggaga agagcgcgtc ggcgacggcc aacgcgccga tgatcagggc
    10621 gaaccggacg acggcggcac ccgcatcggg gcctcccccg ctgatcgcgt tgatcacgtg
    10681 ccccgcgagc accggggtgg ccaccccgat cacggccgcg atcaccacgg tcaccaggaa
    10741 gacgatgatg tcccgccggt aggggtgggc gaacgccatg atccgcttgg cggtgccccg
    10801 ggtgagccgg tgggtggag
//
