LOCUS       NZ_LT607733            10840 bp    DNA     circular CON 17-OCT-2024
DEFINITION  Micromonospora echinofusca strain DSM 43913 chromosome I.
ACCESSION   NZ_LT607733
VERSION     NZ_LT607733.1
KEYWORDS    RefSeq.
SOURCE      Micromonospora echinofusca
  ORGANISM  Micromonospora echinofusca
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Micromonospora.
REFERENCE   1
  AUTHORS   Varghese,Neha. and Submissions,Spin.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2016) DOE - JOINT GENOME INSTITUTE, 2800 Mitchell
            Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900091445.1-RS_2024_10_17
            Annotation Date                   :: 10/17/2024 15:25:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,129
            CDSs (total)                      :: 6,059
            Genes (coding)                    :: 5,948
            CDSs (with protein)               :: 5,948
            Genes (RNA)                       :: 70
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 31 of 111
            Pseudo Genes (incomplete)         :: 94 of 111
            Pseudo Genes (internal stop)      :: 4 of 111
            Pseudo Genes (multiple problems)  :: 17 of 111
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:23:35
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2124813
            Orig. end                         :: 2135653
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LT607733.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10840
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10840
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10840
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5840](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5840)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(696..2408)
                     /db_xref="GeneID:95801782"
                     /locus_tag="GA0070610_RS09640"
                     /old_locus_tag="GA0070610_1962"
     CDS             complement(696..2408)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95801782"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012869.5"
                     /locus_tag="GA0070610_RS09640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070610_1962"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_088999704.1"
                     /transl_table=11
                     /translation="MPTDAVNERDGLLRVVALLRPQWRAAAAAVGLGVLVILLTNALPL
                     VIRHIIDHGLVPRDSDATTDGLWLLLGLAALRWLCDIVRREASGGVGLRLEADLRARLF
                     RHLLSLDVGWHSRVESGRVLSLVGSDVRIIRAFVSYSALFIILNSLTAAAALTQIWLLS
                     WPVGLAATCVLPLFGLVTVGYGRRTFAVSATLQERVAALATHAEENAAGIRVVKGLGRE
                     NEEIRAFTRRSIEVREQNVAASALQARYLPWTIGLPEIGLVLIVWFGGYLTIRGSLTVG
                     TLVALSTYMALLVWPVASMVGLFGSARRAAASAVRVFEVLDRRATMTDRPDAVHLPRPS
                     GGASVTLDDLCFRHPGGTWPGLRRIRLHVMAGEHVTIEGPGGCGKSALAGLLVRLWPPD
                     SGRLLVNGVDVSDVTLASLRGTVGYVPQEVTLLHGTVRDNLLIGRSMATDAQLDRALRL
                     AAADEVIHSLPEGLDTVVGDQGHLLSGGQRQRLALARALLMEPGILVLDDALTQVDMET
                     GRRVHDRLGELVGTVTVLHLTRPGTARHRAGRVVTLTDAGVLRDATEAGGGHR"
     gene            complement(2420..4171)
                     /db_xref="GeneID:95801783"
                     /gene="asnB"
                     /locus_tag="GA0070610_RS09645"
                     /old_locus_tag="GA0070610_1963"
     CDS             complement(2420..4171)
                     /EC_number="6.3.5.4"
                     /GO_function="GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA]"
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95801783"
                     /gene="asnB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /locus_tag="GA0070610_RS09645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070610_1963"
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="WP_088999705.1"
                     /sec_met_domain="GATase_7 (E-value: 8.3e-43, bitscore:
                     143.7, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 2.9e-54, bitscore:
                     183.5, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCGITGIFGSQDRSALTAMTDVLRHRGPDDEGRYDAGVVGVGHRR
                     LSIIGIDNGAQPIRGDRREVYVVANCEIYNYRQLRDELRRRGHDFRTDSDAEVIVHLYE
                     EYGDRCVDHLQGMFAFAIVDGGRLLLARDRFGIKPLHYAEVDGALLFGSEVKALLQNSS
                     LTPEIDGEALVDTVVLGHPAGPGTLLRGVVSLPPGHVLSAVVAEGRLVTALTRYHTLPD
                     VDPEPVPLPEAAAELMDLLRNTVQSHLMAEVEVGVTLSGGLDSTLLARLMRDLHDGPIR
                     AYTIGDRPDHPDVVQARRLARQFGLDHHVSILSFQDFVAAIPRYLTAAEQPAGLGGIAL
                     NVLCRRIGRDVKVCLNGEGADEVFGGYGEYVDRRFRTRRMLDRLAVLETSGRSVSARGR
                     EFLDSLTADQPFPEYLRTLFAENMREQLTRDHLELLDASSMAASLEMRVPFLDHRVVEF
                     AFRQPLAHRVDWELGITKRLLKHAVLTTWGDEVNLVGSVLRRKIGAPTSVLGHRERFIR
                     LCEQVLPVDYLRDDEYGAYFDQKSDLLVFNLYREIFTRYRGVLPDGFTMLDFMTEQARR
                     PALSLRG"
     misc_feature    complement(3482..3484)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4174..5004)
                     /db_xref="GeneID:95801784"
                     /locus_tag="GA0070610_RS09650"
                     /old_locus_tag="GA0070610_1964"
     CDS             complement(4174..5004)
                     /codon_start=1
                     /db_xref="GeneID:95801784"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GA0070610_RS09650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GA0070610_1964"
                     /product="hypothetical protein"
                     /protein_id="WP_088999706.1"
                     /transl_table=11
                     /translation="MSLLDMQRVFSRLLTDPYFQREFFAEGADRPPGYDLTERELDSLR
                     RMDRKRLRLHAGNLAHARLGLALKSTPLSALVLHTQLDRHLVRFCREYPPIPVAESAVI
                     LATTRLCEFAEKLFAEGELQPAYAWDLLRYEKHLLVQGFGVASCASAAEFAHRTTPQGS
                     FGDHVPITGPHVLVDHFRHDLTALLPRLQSGDVPEHVSPLAEPVRILFVKRPLKALVQT
                     CRLNVAMESVWRRCDGVRTVDEIASAYPDPRPVVEALRALHRFGAIALTTGRTC"
     gene            complement(5001..5840)
                     /db_xref="GeneID:95801785"
                     /locus_tag="GA0070610_RS09655"
                     /old_locus_tag="GA0070610_1965"
     CDS             complement(5001..5840)
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95801785"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /locus_tag="GA0070610_RS09655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070610_1965"
                     /product="DUF692 family multinuclear iron-containing
                     protein"
                     /protein_id="WP_088999707.1"
                     /sec_met_domain="DUF692 (E-value: 1.3e-76, bitscore: 255.8,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKALPRLGAGLGYRTALHHDIVANADRIDFLEVISDQYLHAPPE
                     KLLRLLELSDTFPLVPHGVGLSVGTAAPLDDDYLDRLAQFVEATKGYWFSDHLSFTKVP
                     EVDVEQLTPVWFTEESLDAVCRNVRQLKTRIPDPPFLLENVTYYFPVPGNDLSEGAFLT
                     RVLEETDTGLLIDVNNVWINSVNLGFDPYEFLDSIPLERAVQMHIAGGVEMAGMIVDTH
                     SSPVNEHVWQLAAHVVQRAPVKAILLEWDSDWPEFAELLDHLDRARDLLPDRVEAGS"
     misc_feature    complement(5833..5845)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.24"
                     /tool="antismash"
     gene            complement(5921..6052)
                     /db_xref="GeneID:95805991"
                     /locus_tag="GA0070610_RS31675"
                     /old_locus_tag="GA0070610_1966"
     CDS             complement(5921..6052)
                     /codon_start=1
                     /db_xref="GeneID:95805991"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="GA0070610_RS31675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GA0070610_1966"
                     /product="hypothetical protein"
                     /protein_id="WP_269458869.1"
                     /transl_table=11
                     /translation="METKEDIVFEMEELSLSNVPDTDAPGCASCSSCGGCTGCGMEA"
     gene            complement(6114..8186)
                     /db_xref="GeneID:95801786"
                     /locus_tag="GA0070610_RS09660"
                     /old_locus_tag="GA0070610_1967"
     CDS             complement(6114..8186)
                     /codon_start=1
                     /db_xref="GeneID:95801786"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024968.5"
                     /locus_tag="GA0070610_RS09660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070610_1967"
                     /product="glycosyltransferase"
                     /protein_id="WP_088999708.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 2.3e-08,
                     bitscore: 32.3, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTERDNPPGSTRNLTRLQPCPGPSDPLTENSSSPYARFATPLTQR
                     SGSLRICFIGKYPPIEGGVSAQNYWAARALASRGHQVFVVTNANEVEARFRQRLEDGDA
                     EHYEPQFPDSGGAVRVLNPEPMTPRRMTHIPLSNPFVSKLTGLATATIREHDCELIVAY
                     YFEPYVVAGHLASTFTGTPMLVEHAGSDLDRLMRLPELAAVYKEILRSAAAVATKPSLV
                     RRFLGMGVERNALRAGPPYPLPRSAFSPEAEPMSPAEIAAASIDGSGVQLDPALPTIGM
                     YGKPGDRKGTFDLIAAMAILAGRGLRFNALFLSGESQLDRMRSEIRVAGLADRSTILGF
                     VPPWRVARFIRTCTAVCFLERDFPIGIHGPVVPREVLSCGTCLVLSGEILEKQSYAHRL
                     IDQENVLTVADPKEHEKLAAQLELVVRDPDEAARIGAAGGLLGTEFDTFDVFADAWESL
                     VDITSSARGMPAPPHLPDLLAELTPKLAALFGAAWSDVVAGFAATRDATPPADEFAIAE
                     AFCDHLNRHLDQAAAAAAVPPAYLADVLRYQRGRLWASQDGPYDRDVHAGTRFSTPVLR
                     RMVPRRTRTTLVESFDHDVTELFCQPSDPVRAAVGEAGKGCVVCFVRYPNLEHRELRLN
                     DGTRRLLGLCDGTRTVGDIVAALAPAPEIDVVGALVRLRGEGAVVLENAVPDRAP"
     misc_feature    8260..8274
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.46"
                     /tool="antismash"
     misc_feature    complement(8369..8384)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.7"
                     /tool="antismash"
     gene            complement(8645..9589)
                     /db_xref="GeneID:95801787"
                     /locus_tag="GA0070610_RS09665"
                     /old_locus_tag="GA0070610_1968"
     CDS             complement(8645..9589)
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:95801787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013733522.1"
                     /locus_tag="GA0070610_RS09665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GA0070610_1968"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_231926018.1"
                     /transl_table=11
                     /translation="MESADLAACGALLAERHRRHRMAEPLLSARFCAVDAALRELTATF
                     ETTGASGAVALRDAVPVGFVLGAPKSSPVWGPNVWVEAAGQAVTDAEVIRDLYAAAATR
                     WVAEGRTAHYVLVPAHDADLVRAWFRLGFGQQQAHAVMPAVRTRTAEVAGLTVRLAVRA
                     DIPALAELDLELPRQHGSSPTFSPGVLSTVEENLAEWEEGIADAGLATFVAESEGRVVG
                     SAIGCALEKSGTHLGLARPDNAGFLGFAAVFPHARGRGAGRALGEAVIDWAVDSGFDSV
                     VTDWRVTNLLASRTWPALGFTESFLRLHRTVAT"
     misc_feature    9967..9982
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.25"
                     /tool="antismash"
ORIGIN
        1 ctcctcggca tccaccgcgg cgagccgggc cagtgtggcc cgttcgtggc ggaacgcctg
       61 gatgaccggc acggcggtga gcatctgctg cacttgaacg gtcaccgccg tcgcggtctc
      121 ccgcacctgt cgccaggcca ccgcggaccg gcggcggaac catctggtgg ccaacgccag
      181 cacgggcacc acgaccaggc aggcgagggc cagccccgga tcaagcagta cgaggacgac
      241 ggccgtgccg gcgaaggtga gcgtactgac gaagagggtg acgaatccct gggtcagcaa
      301 ccccgtcatc gcctcgatgt cggcggtgag gctagccacc agccggcccg ggcgattgtg
      361 ttcgtggtac tgcatgggca gccgctggag gtgcgagaat agcgccaccc gggcggtgca
      421 cagcatgccg tgtcccgccc gcgcgaccgc gcggacctgg gtgcgggccg caagcacctc
      481 gatgccagcc agcgcgatgc cggccattgc cgccaaggac agcaccccgg cgttcccggt
      541 caggatcccg tcgtcgatgg cgaccttcac caggtacggg atggcgagcg cggcggcggt
      601 ctggacgacg gccgccgcga caccgagcag taccggccct cggtgcgccc gcgccaacgg
      661 tcgcagcaac gcgaccgtgc cccctcgccg agcggtcacc gatgaccacc gcccgcctcg
      721 gtggcgtcgc gcagaacgcc agcatcggtc agcgtcacca cgcggccggc tctgtgtcgc
      781 gcggtgccgg gtcgtgtcag gtgcagcacc gtcaccgtgc cgaccaactc gcccaggcgg
      841 tcgtgcacgc gtcgtccggt ctccatgtcg acctgggtca gcgcgtcgtc gaggacgagg
      901 atcccaggtt ccatcaggag cgcccgggcg agggccaggc gttggcgttg cccgccggag
      961 aggaggtgtc cctgatcgcc gaccacggtg tccagaccct cgggcagcga gtggatcacc
     1021 tcgtcggcag cggcaaggcg cagggcccgg tcgagttgcg cgtcggtggc catggaccgg
     1081 ccgatgagca ggttgtcccg gacggtgccg tgcaacaggg tgacttcctg cgggacgtag
     1141 cccaccgtgc cgcgcaggga ggcgagcgtg acgtcggaga cgtcgacgcc gttgaccagc
     1201 aggcggccgg agtccggcgg ccagagccgt accaggagcc cggccagggc cgacttgccg
     1261 caaccaccgg ggccctcgat cgtcacgtgc tcaccggcca ttacgtgcaa ccggatccgg
     1321 cgcaggcccg gccatgtccc gccgggatgc cggaagcaga ggtcgtcgag ggtgacggaa
     1381 gccccgccgg aaggtcgggg gaggtggacc gcgtcaggac ggtcggtcat cgtcgcgcgt
     1441 cggtccagca cctcgaagac gcgtacggcg ctagcggcgg cccgccgggc ggagccgaac
     1501 aacccgacca tcgaggcgac cggccagacc agcagcgcca tgtaggtgct cagcgccacg
     1561 agggtgccca cagtcagcga tccccggatc gtcaggtagc cgccgaacca gacgatgagc
     1621 accagcccga tctcgggcag cccgatcgtc cagggcaggt aacgggcctg gagagcggag
     1681 gcggccacgt tctgctcgcg aacctcgatc gacctgcggg tgaacgcccg gatttcctcg
     1741 ttctcccggc cgagcccctt gacgacccgg atgcccgccg cgttctcctc ggcgtgcgtg
     1801 gccagcgcgg ccaccctctc ctggagggtg gccgagacgg cgaacgtcct gcggccgtag
     1861 cccacggtga ctagaccgaa cagcggcagg acgcaggtgg cggcgagtcc caccggccag
     1921 gacagcaacc agatctgggt cagggcggcg gcagcggtca gcgagttgag gatgatgaac
     1981 agagcggagt aggaaacgaa ggcgcggatg atccgcacgt cggatccgac gagggagagc
     2041 acccggccgc tctcgacgcg gctgtgccag ccaacgtcga gcgagagcag gtggcggaac
     2101 agccgggccc gcaggtcggc ctccagtcgc agtccgacgc caccgctggc ctcccgacga
     2161 acgatgtcgc agagccaacg cagggcggcc agcccgagca ggagccacag gccgtcggtg
     2221 gtggcgtcgc tgtcgcgggg caccagtccg tgatcgatga tgtgccggat caccaggggc
     2281 agtgcgttgg tgaggaggat gaccaggacg cccaacccga ctgccgccgc ggccgcccgc
     2341 cactggggcc gcagcagtgc cactacgcgg agcaggccat cccgctcgtt gacggcgtcg
     2401 gtcggcatag gactcctggt cagccgcgta gggagagggc cgggcggcgg gcctgctccg
     2461 tcatgaagtc gagcatggtg aacccgtcgg gcagcacgcc ccggtatcgg gtgaagatct
     2521 cccggtacag gttgaacacc agcaggtcgg acttctggtc gaagtaggcg ccgtactcgt
     2581 cgtcgcgcag gtagtccacc ggcagcacct gctcgcagag gcggatgaac cgctcccggt
     2641 gcccgagtac cgaggtgggt gccccgatct tgcggcgcag cacgctgccc accaggttga
     2701 cctcgtcacc ccaggtggtg aggacggcat gcttcaacag gcgcttggtg atgcccaact
     2761 cccagtcgac ccggtgcgca agcggctggc ggaaggcgaa ctcgaccacc cggtggtcga
     2821 ggaagggcac ccgcatctcc aggctcgccg ccatcgagga ggcgtcgagg agctccaggt
     2881 ggtcccgggt gagctgttcc cgcatgttct cggcgaacag cgtccgcagg tactcgggga
     2941 atggctggtc ggccgtgagg gagtccagga actcccggcc ccgagcgctg acgctgcgtc
     3001 ccgacgtttc gaggacggcg agtcggtcga gcatccgacg ggtgcggaac cgccggtcga
     3061 cgtactcgcc gtaaccaccg aacacctcgt ccgcgccctc gccgttgaga cacaccttca
     3121 catcgcggcc gatgcgtcgg cagaggacgt tgagtgcgat cccgccgaga ccggcgggct
     3181 gttcagcggc cgtgaggtag cgggggatgg cggcgacaaa gtcctggaag ctcaggatcg
     3241 acacatggtg gtccagtccg aactgccggg ccagccgtcg ggcctgcacc acgtcgggat
     3301 ggtccggacg gtccccgatc gtgtacgcgc ggatcgggcc gtcgtgcagg tcgcgcatga
     3361 gccgtgccag gagcgtcgag tcgagcccgc cggagagcgt gacgccgacc tcgacctcgg
     3421 ccatcaggtg cgattggacg gtgttccgca gcaggtccat gagttcggcg gcggcctcgg
     3481 gtaacgggac cggctcgggg tcgacgtccg gcagcgtgtg gtagcgggtg agcgcggtga
     3541 ccagccggcc ctctgccacg accgcgctga gcacgtggcc gggcggcagg ctcaccaccc
     3601 cgcgcagcag ggtgcccggg ccggcgggat ggccgagcac gacggtgtcg accagcgcct
     3661 cgccgtcgat ctcgggcgtc agcgaggagt tctgtagcag ggccttgacc tcggatccga
     3721 acagcagggc gccgtcgacc tcggcgtagt gcagcggttt gatgccgaat cggtcgcggg
     3781 ccagcagcaa ccgtccaccg tcaacgatcg cgaaggcgaa catgccctgg agatgatcga
     3841 cgcagcggtc gccgtactcc tcgtacaggt gcacgatgac ctcggcgtcc gagtcggtac
     3901 ggaagtcgtg tccccgccgc cgcaactcgt cccggagctg tcgatagttg tagatctcgc
     3961 agttggccac cacgtacacc tcgcggcggt cgccgcggat cggttgggcg ccgttgtcga
     4021 ttccgatgat gctcaggcgt cgatgaccga cgcccacgac gcccgcgtcg taccggccct
     4081 cgtcgtcggg gccgcggtgg cgcagtacgt ccgtcatcgc cgtcagagcc gaccggtcct
     4141 gactgccgaa gattccggtg ataccgcaca tcctcagcac gtccttccgg tcgtcagcgc
     4201 gatcgccccg aaccggtgca gggcgcggag cgcctcgacg accgggcgcg ggtcggggta
     4261 ggcagaagcg atctcgtcca cggtgcggac gccgtcgcac cgccgccaca ccgactccat
     4321 cgcgacgttg agccgacagg tctgcacgag ggccttcagc ggccgcttga cgaacaggat
     4381 ccgtaccggc tcggccaacg gcgacacgtg ctcgggcaca tcaccggact gcaaccttgg
     4441 cagaagggcg gtcaggtcgt gtcggaaatg gtcgacgagc acatgggggc cggtgatcgg
     4501 cacgtggtcg ccgaacgacc cttgcggggt ggtccggtgc gcgaactcgg cggcactggc
     4561 acaggacgcg acgccgaacc cctgcaccag caggtgtttc tcgtaccgga gcagatccca
     4621 cgcgtacgcg ggctgcaact cgccctcggc gaacagcttc tccgcgaact cgcacagccg
     4681 ggtggtggcc aggatgacgg cgctctccgc gaccgggatc gggggatact cccgacagaa
     4741 gcggaccagg tggcggtcca gttgggtgtg caggaccagc gccgacaacg gcgtcgactt
     4801 gagcgccagg cccagccggg cgtgcgcgag gttgccggcg tgcagccgca gtcgcttccg
     4861 gtccatccgc cgcaggctgt ccagctcccg ttcggtgagg tcgtatcccg gcggccggtc
     4921 cgcgccctcg gcgaagaact cccgctggaa gtacggatcg gtgagtagcc ggctgaacac
     4981 ccgctgcatg tcgagcaggc tcatgagccg gcttccaccc ggtcgggcag caggtcccgg
     5041 gcgcggtcca ggtggtcgag cagctctgcg aactccggcc agtcagagtc ccactcgagc
     5101 aggatcgcct ttaccggggc gcgttgcacc acatgggccg ccagctgcca gacatgctcg
     5161 ttgacggggc tgctgtgggt gtcgacgatc atcccggcca tctcgacccc gccggcgatg
     5221 tgcatctgca ccgcccgctc cagcggtatg gagtcgagga actcgtacgg gtcgaagccc
     5281 aggttcaccg agttgatcca cacgttgttc acgtcgatca gtaggccggt gtcggtttcc
     5341 tccagtaccc gggtgagaaa cgcgccctcg ctgaggtcgt tgccggggac ggggaagtag
     5401 taggtgacgt tttccagcag gaagggcgga tccgggattc gtgtcttcaa ttgccggaca
     5461 tttcggcaca cggcgtcgag gctctcttcg gtgaaccata ccggagtgag ttgctcaaca
     5521 tcgacctcgg gcacctttgt gaagctgagg tggtcgctga accagtagcc cttcgtggcc
     5581 tccacgaact gcgcgagcct atccaggtaa tcgtcgtcga gaggcgcggc cgtgcctacg
     5641 gagaggccaa cgccgtgggg aaccaacgga aaggtgtcgc tgagttccag aagccgcagg
     5701 agcttctccg gcggggcgtg gagatactgg tcggaaatta cctcgagaaa gtcgattcgg
     5761 tccgcgttgg cgacgatgtc gtggtgcagc gccgtgcggt accccaggcc ggcgccgagc
     5821 cgtggtagag ccttgctcat tgcatcctcc tgtggaaaat tgtgaggagt gcgggctgcg
     5881 gtgccggcct agggcatcag cggcttgggt cagaaggcgg tcaggcctcc ataccgcagc
     5941 ccgtgcaccc accgcagctc gagcaactcg cgcagccggg ggcgtcggtg tcggggacgt
     6001 tgctgagcga gagttcctcc atctcgaaca cgatgtcctc cttggtttcc atgtggttca
     6061 cctcccttcg tggacgagtg gccgaccgac ggtgcggacg cactgccggg gtctcagggg
     6121 gcgcggtccg ggacggcgtt ctccagcacc accgcgccct cgccgcgcag gcgcacgagg
     6181 gcgccgacga cgtcgatctc cggggctggg gccagcgcgg cgacgatgtc gcccacggtg
     6241 cgggtaccgt cgcagaggcc gagtaggcga cgggtgccgt cgttgaggcg caactcgcgg
     6301 tgctccaggt tggggtaccg gacgaaacag acgacgcagc ctttgcccgc ctcgccgaca
     6361 gcggcgcgga ccgggtcgga cggctggcag aacaactcgg tcacgtcgtg gtcgaacgac
     6421 tccaccaggg tcgtgcgggt gcggcgcggc accatccgcc gcaggaccgg cgtcgagaat
     6481 cgggtgccgg cgtgcacgtc ccggtcgtac gggccgtcct gactggccca gagccggccc
     6541 cgctggtagc gcagcacgtc ggcgaggtaa gccgggggaa cggcggcagc cgccgcggcc
     6601 tggtccagat gccggttcag gtggtcacag aacgcctcgg cgatggcgaa ctcgtcggcc
     6661 ggcggtgtgg cgtcccgcgt cgccgcgaac ccggcgacga cgtcggacca ggctgcgccg
     6721 aacaacgcgg ccaacttcgg tgtcagctcg gccagcaggt cgggcaggtg tgggggtgcg
     6781 ggcatccccc gggccgacga agtgatgtcg accagcgact cccaggcgtc ggcgaagacg
     6841 tcgaaggtgt cgaactcggt gccgagcaga cctcccgccg cgccgatgcg ggcggcctcg
     6901 tcggggtcgc ggacgaccag ttcgagttgc gcagccagct tctcgtgctc cttggggtca
     6961 gcgacggtca ggacgttctc ctggtcgatc aggcggtggg cgtacgactg cttctcgagg
     7021 atctccccgg acagcaccag gcaggtgccg caggaaagca cctcccgcgg tacgaccgga
     7081 ccatgtatgc cgatcggaaa gtcccgctcc aggaagcaca cggccgtgca ggtacggatg
     7141 aaccgggcca cccgccacgg cgggacgaac ccgaggatgg tgctccggtc ggccaggccg
     7201 gcaacgcgga tctccgagcg catccggtcg agctgggatt caccactgag gaatagcgcg
     7261 ttgaagcgaa gtcctcggcc ggcgaggatg gccatggccg cgatcaggtc gaacgtgccc
     7321 ttccggtcgc cgggcttgcc gtacatgccg atggtcggta gcgccgggtc gagctggacg
     7381 ccggagccgt cgatggacgc ggcggcgatc tcggcggggc tcatcggctc ggcttcgggt
     7441 gagaacgccg atcgtggcag cgggtagggc ggaccggccc gcagcgcgtt gcgctcgaca
     7501 cccatgccga ggaaccggcg taccagggag ggtttggtcg cgacggcggc ggccgagcgc
     7561 aggatctcct tgtagaccgc cgccagctcg ggcaggcgca tgagccggtc gaggtcgcta
     7621 ccggcatgct cgaccagcat cggcgtgccg gtgaacgtcg acgcgaggtg cccggccacc
     7681 acgtacggct cgaagtagta ggcgacgatc agttcgcagt cgtgttcgcg aatcgtcgcg
     7741 gtggccaggc cggtgagctt cgagacgaat gggttgctca acgggatgtg tgtcatccgg
     7801 cgcggcgtca taggctccgg gttcagcacc ctgaccgcgc cgcccgaatc ggggaattgc
     7861 ggttcgtagt gctccgcgtc cccgtcctcg agacgctgcc ggaaacgcgc ctcgacctcg
     7921 ttcgcgttcg ttaccacgaa tacttggtga ccgcgggacg ccaaagctct cgcggcccag
     7981 tagttctggg cactgacgcc gccctcgatc ggaggatact tgccgataaa acatatcctc
     8041 aaggatccgc tcctctgggt gaggggagtt gcgaagcgag cgtaggggga gctcgagttt
     8101 tccgtcaacg gatccgaggg tccggggcag ggctgaaggc gcgtcaaatt gcgggtagac
     8161 cccggagggt tgtcacgctc ggtcatgtcc ggccgaatgg atcaacagtc tgagtgccgg
     8221 gacaggaaaa gctccggaaa cccccggtca accggaatcg cacaacctgt cgggcattcg
     8281 cgtccaggcc gccggccagg gttgtctgtc cgacatgcgt gacatcggcc agtgacgtcc
     8341 gggccacgga tcgttacggt tgtggcggga gaatgcaaag cattggtaag ccgctggcca
     8401 aacaggtcat cgggaacagg aggagccatt ccctgctagc ccgagcccac ttctagcctg
     8461 ttcggcgcta gttgcgcact gcgggatacc ggacaagacg cccgccggat cgtgcgcccg
     8521 gagggcttcc cgcgggtgca cgccgatcgg acggaccgcc gggaggcccc gccaggacgc
     8581 cgccaccacg tcggtctccc gtcgatcccg ccagggcggc gtgcgttcgg tgccgacgag
     8641 agggtcaggt ggcgacggta cggtgcagcc ggaggaagga ctccgtgaac ccgagtgccg
     8701 gccaggtgcg cgacgcgagc aggttcgtca cccgccagtc ggtcaccacg ctgtcgaatc
     8761 cactgtcgac ggcccagtcg atgacggcct cgccgagtgc ccggcccgcg cccctgcccc
     8821 gggcgtgggg gaagacggca gcgaagccga ggaacccggc gttgtccggg cgggccagac
     8881 ccagatgcgt acccgacttc tccagagcgc atccgatggc cgaaccgacc acgcggcctt
     8941 ccgactcggc cacgaacgtg gcgagtccgg cgtcggcgat gccctcctcc cactcggcca
     9001 ggttctcctc gacggtggac agcacgccgg gggagaacgt cggggacgat ccgtgctgcc
     9061 gggggagttc gaggtcgagt tcggcgagtg ccgggatgtc cgcacgaaca gccagacgca
     9121 ccgtcagtcc cgcgacctcg gcggtccgcg tcctgaccgc aggcatgacg gcgtgcgcct
     9181 gctgctgccc gaaaccgagt cggaaccagg cccggacgag gtcggcgtcg tgcgccggca
     9241 ccagcacgta gtgggccgtc cggccttctg cgacccatcg ggtcgccgcc gcggcgtaca
     9301 ggtcccgtat cacctctgcg tcggtcaccg cctgccccgc cgcttccacc cagacgttcg
     9361 gcccccacac cggggacgat ttcggggctc cgaggacgaa gccgaccgga acggcgtcgc
     9421 gcagtgccac cgcgcccgac gcgccggtcg tctcgaacgt ggcggtcagc tcgcgcagcg
     9481 cggcgtcgac cgcacagaac cgcgcggaca gcagcggttc ggccatccgg tgccggcggt
     9541 gccgttcggc cagcagcgcg ccgcaggcgg ccagatcagc ggactccatc ggccgtactc
     9601 tgactcggga catgtcccgt gacggtagcc accggcccgt ccgtcggcat cggattttcg
     9661 ggacgggcag cggcaggcag cacgtcacga gccacgcccc ggtgccctgt gtcggatccc
     9721 gcctacgccg gtaccgcctg cggatcctcg gcggcgtcgt gcgcggccat ggtctcctgc
     9781 cgcaggggtt cctcgcgcag gatggcgcag tgcagccacg cgtccgggat gccgaaaccg
     9841 tccacgaggg tccgcatgtg cgggcgcagc tccttgagca gatcgttcac cacgccggtg
     9901 atcgccttgg aacgggccgg cgtgagacgg ccgtgctcca ggaaccatcc cttgtcggcc
     9961 tcgatgacgc tgagcgcgta caggtcgcag acccgggaga gcagcgcccg gacggacggg
    10021 tcggtggcgt cctcgatgcc ggcgacgaac gcctccaggg tgacccggtc gacgtgcgcg
    10081 gcggcgacgg cgaggacgtg gtcctggacg tcgttgaaga tgtcgaacgg gcggtcctgc
    10141 ttcgtggccg cgccgttgcg caggcgccgg acggcgccgt cgaggagatg cctctcgcgg
    10201 tcctcgaaca tcttgagctg ccagccgcgg tcggtgacgg cgacctcgtc gtcgcggccg
    10261 ggcacggcgc tgatcatccg ctcgatgagc gagcgcgcgg cggtgcgctc gagcaccatc
    10321 tcgcggacct gttcggcgac gaacgaggcg cgcccccagc cgtccaacga gccgaactcg
    10381 tcccggtagc cggtgagcag ccccttggcc accagttgca gcagcaccgt gttgtcgccc
    10441 tcgaaggtgg tgaagacgtc ggtgtcggcc ttgaggctgg gtagccggtt ctcggacagg
    10501 tagccggcgc cgccgcacgc ctcgcggcac atctggatgg tgcgggtcgc gtgccaggtc
    10561 tgcgcggcct tgagaccggc ggcccgggac tccagctccc gttgccggtg ctcgtcgacc
    10621 ggctcgtcgc cgccctgcgc ctcgctcagc gcggccacca gctcggcctg ggcgaagtgc
    10681 aacgcgtacg tggtggccag ggcgggcagc agcttgcgtt ggtgggccag gtagtcgttg
    10741 agcagcacct cccgctccgc gtcgggcgtg ccgaactggc ggcggatgtc gccgtagcgc
    10801 accgcgatgg ccagcgccga cctggtggcc gccgacgcgg
//
