GCF_900090125.1 - 26 region(s)

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Location: 1 - 2,995 nt. (total: 2,995 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene overview

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MIBiG comparison

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NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase

H181DRAFT_RS28170: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.61	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.60	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.60	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.61	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	0.60	NRPS	AbT1	Aureobasidium pullulans
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001833.4	0.60	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.60	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.60	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.60	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

NRPS/PKS substrate predictions

H181DRAFT_RS28170: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (491..889): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: LWATFDVSVFESSTLYAGELNVYGPTEATVYATM
Nearest Stachelhaus code(s):: Ala DVFSLAVVYK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

NZ_FMHJ01000007.1 - Region 1 - linaridin

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Location: 64,627 - 85,205 nt. (total: 20,579 nt) Show pHMM detection rules used

linaridin: (cypemycin or cypI)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: hydratase

H181DRAFT_RS14485: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Isomerases: racemase

H181DRAFT_RS14510: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

Translocases: transporter

H181DRAFT_RS14515: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001735.5	0.48	other (other)	pentostatine, vidarabine	Streptomyces antibioticus
BGC0000913.3	0.39	other (other)	MK-8	Enterobacter cloacae
BGC0002034.3	0.37	other (other)	perquinoline A, perquinoline B, perquinoline C	Streptomyces sp. IB2014 016-6
BGC0001782.3	0.36	other (other)	spiroindimicin A, spiroindimicin B, spiroindimicin C, spiroindimicin D, indimicin A, indimicin B, indimicin C, indimicin D, indimicin E, lynamicin A, lynamicin D, lynamicin F, lynamicin G	Streptomyces sp. SCSIO 03032
BGC0002346.3	0.35	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0002669.2	0.35	ribosomal	TVA-YJ-2	Streptomyces sp. NRRL S-87
BGC0000842.5	0.34	other (non-nrp beta-lactam)	carbapenem MM4550	Streptomyces argenteolus
BGC0000197.5	0.34	PKS, saccharide	aranciamycin	Streptomyces echinatus
BGC0002553.2	0.34	other (other)	tunicamycin	Rathayibacter iranicus
BGC0001379.5	0.34	NRPS	BD-12	Streptomyces luteocolor

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001735.5	0.48	other (other)	pentostatine, vidarabine	Streptomyces antibioticus
BGC0000913.3	0.39	other (other)	MK-8	Enterobacter cloacae
BGC0002034.3	0.37	other (other)	perquinoline A, perquinoline B, perquinoline C	Streptomyces sp. IB2014 016-6
BGC0001782.3	0.36	other (other)	spiroindimicin A, spiroindimicin B, spiroindimicin C, spiroindimicin D, indimicin A, indimicin B, indimicin C, indimicin D, indimicin E, lynamicin A, lynamicin D, lynamicin F, lynamicin G	Streptomyces sp. SCSIO 03032
BGC0002346.3	0.35	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0002669.2	0.35	ribosomal	TVA-YJ-2	Streptomyces sp. NRRL S-87
BGC0000842.5	0.34	other (non-nrp beta-lactam)	carbapenem MM4550	Streptomyces argenteolus
BGC0000197.5	0.34	PKS, saccharide	aranciamycin	Streptomyces echinatus
BGC0002553.2	0.34	other (other)	tunicamycin	Rathayibacter iranicus
BGC0001379.5	0.34	NRPS	BD-12	Streptomyces luteocolor

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (2):

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.14 out of 19.26

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.03 out of 19.26

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_FMHJ01000005.1 - Region 1 - PKS-like,T1PKS,butyrolactone,t3nrps-iterative

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Location: 236,309 - 298,684 nt. (total: 62,376 nt) Show pHMM detection rules used

PKS-like: (t2pks2 or ksIII)
t3nrps-iterative: (AMP-binding and PP-binding and (DUF98 or Orn_Arg_deC_N or Orn_DAP_Arg_deC) and not ATP-grasp)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

H181DRAFT_RS11290: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

H181DRAFT_RS11300: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

H181DRAFT_RS29400: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

H181DRAFT_RS29405: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Transferases: PPTase

H181DRAFT_RS11350: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: esterase phosphatase

H181DRAFT_RS11255: thioesterase

smcogs: SMCOG1004: thioesterase

H181DRAFT_RS11305: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

Lyases: decarboxylase

H181DRAFT_RS11275: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Ligases: ligase synthase

H181DRAFT_RS11265: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

H181DRAFT_RS11270: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS11310: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS11330: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

H181DRAFT_RS11335: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Translocases: transport transporter

H181DRAFT_RS11215: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

H181DRAFT_RS11285: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.93	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.91	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.90	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.90	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001250.4	0.86	NRPS	epichloenin A	Epichloe festucae
BGC0001251.4	0.86	NRPS	epichloenin A	Epichloe festucae
BGC0000307.4	0.86	NRPS	AbT1	Aureobasidium pullulans
BGC0001164.3	0.86	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.86	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001284.4	0.86	PKS	alternariol	Parastagonospora nodorum SN15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.65	PKS	asperlactone	Aspergillus ochraceus
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.64	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0002154.2	0.63	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15
BGC0002175.3	0.63	PKS	YWA1	Aspergillus oryzae RIB40
BGC0001257.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0001258.3	0.63	PKS	1,3,6,8-tetrahydroxynaphthalene	Glarea lozoyensis
BGC0000107.3	0.63	PKS	naphthopyrone	Aspergillus nidulans FGSC A4
BGC0001250.4	0.62	NRPS	epichloenin A	Epichloe festucae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.46 out of 20.47

Confidence: strong

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 20.76 out of 21.84

Confidence: strong

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.85 out of 20.97

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.24 out of 19.26

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

Additional weak hits (10):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.19 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.71 out of 27.78

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.46 out of 32.93

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.78 out of 29.65

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 13.15 out of 18.57

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.10 out of 35.63

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.27 out of 30.12

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.27 out of 30.12

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.27 out of 30.12

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 236308 - 298684:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 237372 - 298684:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

H181DRAFT_RS11270: X

AMP-binding (27..426): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AAVVFDYGLYQLPLTNTGAAPMYGTTECKRITIL
Nearest Stachelhaus code(s):: Asp DYYPTTMRVK (74% 8Å match)
Stachelhaus code match:: 90% (moderate)

H181DRAFT_RS11310: NH2

CAL_domain (40..484): NH2

minowa: NH2

Prediction, score:

NH2: 162.5
fatty_acid: 45.3
AHBA: 13.2
shikimic_acid: 6.7
Acetyl-CoA: -1.8

H181DRAFT_RS11330: mal

PKS_AT (528..810): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 151.2
inactive: 84.7
Methoxymalonyl-CoA: 79.7
Methylmalonyl-CoA: 67.8
Propionyl-CoA: 57.2
Ethylmalonyl-CoA: 45.5
2-Methylbutyryl-CoA: 37.9
trans-1,2-CPDA: 28.2
fatty_acid: 25.5
Isobutyryl-CoA: 24.4
Acetyl-CoA: 20.3
Benzoyl-CoA: 17.8
CHC-CoA: 14.7
3-Methylbutyryl-CoA: 0.0

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
MexT	Global virulence regulator
VqsM	Regulator of quorum-sensing signalling systems

NZ_FMHJ01000005.1 - Region 2 - ectoine

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Location: 420,917 - 431,321 nt. (total: 10,405 nt) Show pHMM detection rules used

ectoine: ectoine_synt

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

H181DRAFT_RS11945: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

Transferases: aminotransferase

H181DRAFT_RS11965: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002052.3	0.86	other (ectoine)	ectoine	Streptomyces sp.
BGC0000853.3	0.85	other (other)	ectoine	Streptomyces anulatus
BGC0000856.3	0.72	other (other)	ectoine	Methylophaga thalassica
BGC0000852.5	0.70	other (other)	ectoine	Sporosarcina pasteurii
BGC0000858.3	0.66	other (other)	ectoine	Methylobacter marinus
BGC0000859.3	0.66	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000855.3	0.66	other (other)	ectoine	Methylomicrobium kenyense
BGC0000857.3	0.63	other (other)	ectoine	Methylophaga alcalica
BGC0000860.3	0.63	other (other)	ectoine	Methylarcula marina
BGC0000854.3	0.62	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002052.3	0.86	other (ectoine)	ectoine	Streptomyces sp.
BGC0000853.3	0.85	other (other)	ectoine	Streptomyces anulatus
BGC0000856.3	0.72	other (other)	ectoine	Methylophaga thalassica
BGC0000852.5	0.70	other (other)	ectoine	Sporosarcina pasteurii
BGC0000858.3	0.66	other (other)	ectoine	Methylobacter marinus
BGC0000859.3	0.66	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000855.3	0.66	other (other)	ectoine	Methylomicrobium kenyense
BGC0000857.3	0.63	other (other)	ectoine	Methylophaga alcalica
BGC0000860.3	0.63	other (other)	ectoine	Methylarcula marina
BGC0000854.3	0.62	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.93 out of 23.79

Confidence: medium

Additional weak hits (5):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 18.45 out of 27.78

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.64 out of 31.73

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.03 out of 19.26

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.08 out of 27.18

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.79 out of 36.53

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_FMHJ01000010.1 - Region 1 - RiPP-like

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Location: 45,438 - 56,710 nt. (total: 11,273 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

H181DRAFT_RS18750: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001972.4	0.36	other (other)	toxoflavin, fervenulin	Streptomyces hiroshimensis
BGC0000306.5	0.31	NRPS	arylomycin	Streptomyces filamentosus NRRL 11379
BGC0002303.3	0.30	ribosomal	pristinin A3	Streptomyces pristinaespiralis
BGC0002387.2	0.30	PKS	yangpumicin A	Micromonospora yangpuensis
BGC0000466.5	0.28	NRPS	yatakemycin	Streptomyces sp. TP-A2060
BGC0001377.5	0.28	PKS	uncialamycin	Streptomyces uncialis
BGC0000874.5	0.27	other (other)	blasticidin S	Streptomyces griseochromogenes
BGC0001060.5	0.26	PKS	dynemicin A	Micromonospora chersina
BGC0002788.2	0.26	PKS	14-hydroxyisochainin	Streptomyces peucetius subsp. caesius ATCC 27952
BGC0002408.3	0.25	NRPS	malleobactin X	Collimonas pratensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001972.4	0.36	other (other)	toxoflavin, fervenulin	Streptomyces hiroshimensis
BGC0000306.5	0.31	NRPS	arylomycin	Streptomyces filamentosus NRRL 11379
BGC0002303.3	0.30	ribosomal	pristinin A3	Streptomyces pristinaespiralis
BGC0002387.2	0.30	PKS	yangpumicin A	Micromonospora yangpuensis
BGC0000466.5	0.28	NRPS	yatakemycin	Streptomyces sp. TP-A2060
BGC0001377.5	0.28	PKS	uncialamycin	Streptomyces uncialis
BGC0000874.5	0.27	other (other)	blasticidin S	Streptomyces griseochromogenes
BGC0001060.5	0.26	PKS	dynemicin A	Micromonospora chersina
BGC0002788.2	0.26	PKS	14-hydroxyisochainin	Streptomyces peucetius subsp. caesius ATCC 27952
BGC0002408.3	0.25	NRPS	malleobactin X	Collimonas pratensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.50 out of 26.85

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.79 out of 19.52

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.32 out of 39.62

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_FMHJ01000001.1 - Region 1 - NRP-metallophore,NRPS,T1PKS

Download region SVG

Location: 1 - 91,917 nt. (total: 91,917 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

H181DRAFT_RS00060: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

H181DRAFT_RS00085: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

H181DRAFT_RS00090: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

H181DRAFT_RS00160: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: PPTase aminotransferase

H181DRAFT_RS00185: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

H181DRAFT_RS00195: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1060: 4'-phosphopantetheinyl transferase

Hydrolases: esterase hydrolase

H181DRAFT_RS00100: thioesterase

smcogs: SMCOG1004: thioesterase

H181DRAFT_RS00125: thioesterase

smcogs: SMCOG1004: thioesterase

H181DRAFT_RS00175: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

H181DRAFT_RS00205: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Isomerases: isomerase

H181DRAFT_RS00115: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

H181DRAFT_RS00050: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS00065: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

H181DRAFT_RS00070: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

H181DRAFT_RS00075: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS00080: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

H181DRAFT_RS00105: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

H181DRAFT_RS00145: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

H181DRAFT_RS00150: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

H181DRAFT_RS00155: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS00180: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	1.58	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	1.54	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001164.3	1.53	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001132.4	1.53	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000342.4	1.51	NRPS	enniatin	Fusarium equiseti
BGC0001160.5	1.51	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001758.4	1.48	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	1.45	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	1.42	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000307.4	1.39	NRPS	AbT1	Aureobasidium pullulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001164.3	0.74	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.73	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0000412.5	0.73	NRPS	pyochelin	Pseudomonas aeruginosa PAO1
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.73	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001050.5	0.73	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0001825.4	0.72	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	0.72	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.29 out of 23.79

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

Additional weak hits (19):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.54 out of 26.85

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.99 out of 26.33

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.37 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.42 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.95 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.55 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.73 out of 27.78

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.00 out of 29.38

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.03 out of 19.26

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.54 out of 20.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.20 out of 27.78

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.94 out of 27.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.82 out of 30.25

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.61 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.40 out of 35.63

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.98 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.18 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 91917:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala - D-Val) + (NH2 - Me-redmal) + (mal) + (Ser) + (ccmal) + (Me-Cys) + (Cys)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

H181DRAFT_RS00050: Ala - Val

Search NORINE for peptide: strict or relaxed

AMP-binding (500..892): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAMTFDIATLELQALICGEWNMYGPSEATIYALG
Nearest Stachelhaus code(s):: Ala DILQLCMIYK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1559..1961): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDHAFDASTYEVFFMAGGETNGYGPTETTTFAVL
Nearest Stachelhaus code(s):: Val DAYFLGGTFK (97% 8Å match)
Stachelhaus code match:: 90% (moderate)

H181DRAFT_RS00065: NH2 - mmal

CAL_domain (42..487): NH2

minowa: NH2

Prediction, score:

NH2: 146.8
Acetyl-CoA: 65.7
fatty_acid: 19.6
AHBA: 10.7
shikimic_acid: 0.0

PKS_AT (1242..1544): mmal

ATSignature: Malonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 70.8%; Propionyl-CoA: 62.5%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 106.5
Methoxymalonyl-CoA: 101.1
Malonyl-CoA: 87.8
Isobutyryl-CoA: 73.2
Ethylmalonyl-CoA: 65.9
3-Methylbutyryl-CoA: 56.6
Propionyl-CoA: 56.2
Benzoyl-CoA: 47.0
CHC-CoA: 42.1
2-Methylbutyryl-CoA: 39.1
inactive: 38.2
trans-1,2-CPDA: 34.9
Acetyl-CoA: 23.9
fatty_acid: 21.6

H181DRAFT_RS00070: mal

PKS_AT (563..859): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 158.4
inactive: 115.5
Methoxymalonyl-CoA: 81.2
Methylmalonyl-CoA: 69.4
Propionyl-CoA: 40.2
Ethylmalonyl-CoA: 36.0
2-Methylbutyryl-CoA: 33.6
CHC-CoA: 26.9
Isobutyryl-CoA: 21.1
trans-1,2-CPDA: 20.5
Benzoyl-CoA: 19.8
Acetyl-CoA: 18.3
fatty_acid: 14.3
3-Methylbutyryl-CoA: 3.9

H181DRAFT_RS00075: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (493..890): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFLCSGEHNLYGPTEAAIDVTA
Nearest Stachelhaus code(s):: Ser DVWHLSLIDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS00080: mal

PKS_AT (546..838): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 171.8
inactive: 86.8
Methoxymalonyl-CoA: 71.5
Methylmalonyl-CoA: 65.0
Propionyl-CoA: 55.1
Ethylmalonyl-CoA: 37.4
Benzoyl-CoA: 35.1
Isobutyryl-CoA: 31.7
CHC-CoA: 31.5
Acetyl-CoA: 25.5
2-Methylbutyryl-CoA: 23.8
fatty_acid: 16.6
trans-1,2-CPDA: 16.3
3-Methylbutyryl-CoA: 0.0

H181DRAFT_RS00145: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (548..942): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDIAFDLSVYDGNSLLSGDVSLGGATEAAIWSIH
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS00150: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (566..960): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDVSFDLSVWDGNSLLSGDVGLGGATEAAIWSNC
Nearest Stachelhaus code(s):: Cys DLWNLSLIWK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS00155: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (34..430): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: QLTLFPLACPGGSLQVGGAQQVYGMAEGLLNFTR
Nearest Stachelhaus code(s):: Sal ALPSQGVLNK (91% 8Å match); diOH-Bz PLPSQGVVNK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

H181DRAFT_RS00180: NH2

CAL_domain (162..481): NH2

minowa: NH2

Prediction, score:

NH2: 75.2
fatty_acid: 42.5
Acetyl-CoA: 20.2
shikimic_acid: 0.0
AHBA: 0.0

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
IolR	Regulator of the inositol catabolism

NZ_FMHJ01000001.1 - Region 2 - butyrolactone

Download region SVG

Location: 161,810 - 172,793 nt. (total: 10,984 nt) Show pHMM detection rules used

butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: dehydratase

H181DRAFT_RS00525: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000849.4	0.77	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000848.5	0.76	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000850.5	0.69	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0002578.2	0.38	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712
BGC0001458.4	0.33	other (aminocoumarin)	salinipostin G, Salinipostin G, Salinipostin A, Salinipostin B, Salinipostin C, Salinipostin D, Salinipostin E, Salinipostin F, Salinipostin H, Salinipostin I, Salinipostin J, Salinipostin K	Salinispora tropica CNB-440
BGC0000914.5	0.28	other (other)	methylenomycin A	Streptomyces coelicolor A3(2)
BGC0001083.5	0.28	PKS, terpene	merochlorin A, merochlorin B, deschloro-merochlorin A, deschloro-merochlorin B, isochloro-merochlorin B, dichloro-merochlorin B, merochlorin D, merochlorin C	Streptomyces sp. CNH189
BGC0000112.5	0.26	PKS	neocarzinostatin	Streptomyces carzinostaticus subsp. neocarzinostaticus
BGC0002642.2	0.26	other (other)	clipibycyclene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000262.5	0.25	PKS, saccharide	prejadomycin, rabelomycin, gaudimycin C, gaudimycin D, UWM6, gaudimycin A	Streptomyces sp. PGA64

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000849.4	0.77	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000848.5	0.76	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000850.5	0.69	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0002578.2	0.38	other (other)	gaburedin A, gaburedin B, gaburedin C, gaburedin D, gaburedin E, gaburedin F	Streptomyces venezuelae ATCC 10712
BGC0001458.4	0.33	other (aminocoumarin)	salinipostin G, Salinipostin G, Salinipostin A, Salinipostin B, Salinipostin C, Salinipostin D, Salinipostin E, Salinipostin F, Salinipostin H, Salinipostin I, Salinipostin J, Salinipostin K	Salinispora tropica CNB-440
BGC0000914.5	0.28	other (other)	methylenomycin A	Streptomyces coelicolor A3(2)
BGC0001083.5	0.28	PKS, terpene	merochlorin A, merochlorin B, deschloro-merochlorin A, deschloro-merochlorin B, isochloro-merochlorin B, dichloro-merochlorin B, merochlorin D, merochlorin C	Streptomyces sp. CNH189
BGC0000112.5	0.26	PKS	neocarzinostatin	Streptomyces carzinostaticus subsp. neocarzinostaticus
BGC0002642.2	0.26	other (other)	clipibycyclene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000262.5	0.25	PKS, saccharide	prejadomycin, rabelomycin, gaudimycin C, gaudimycin D, UWM6, gaudimycin A	Streptomyces sp. PGA64

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (5):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.10 out of 29.25

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.09 out of 26.06

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.42 out of 27.55

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.62 out of 39.62

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.11 out of 26.33

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description

NZ_FMHJ01000001.1 - Region 3 - RRE-containing,azole-containing-RiPP

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Location: 295,105 - 330,178 nt. (total: 35,074 nt) Show pHMM detection rules used

azole-containing-RiPP: ((YcaO or TIGR03604) and (((thio_amide and (PF06968 or PF04055 or PF07366)) or Lant_dehydr_C or Lant_dehydr_N or (PF06968 or PF04055 or PF07366)) or (goadsporin_like or PF00881 or TIGR03605)) or TIGR03882 or TIGR03603 or thiostrepton or TIGR03602)
RRE-containing: minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE])

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Azole RiPPs

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

H181DRAFT_RS01135: amidohydrolase

smcogs: SMCOG1244: amidohydrolase

Lyases: dehydratase

H181DRAFT_RS01165: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

H181DRAFT_RS01170: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001146.5	0.42	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0000603.5	0.35	ribosomal	cyclothiazomycin	Streptomyces hygroscopicus
BGC0001145.5	0.34	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0000607.5	0.32	ribosomal	micrococcin P1	Macrococcus caseolyticus
BGC0001640.3	0.31	ribosomal	kocurin	Kocuria rosea
BGC0000606.5	0.31	ribosomal	lactazole	Streptomyces lactacystinaeus
BGC0001860.4	0.30	ribosomal	thiomuracin	Thermobispora bispora DSM 43833
BGC0000566.5	0.28	ribosomal	streptolysin S	Streptococcus pyogenes M1 GAS
BGC0000613.5	0.28	ribosomal	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0000628.4	0.28	ribosomal	lactocillin	Lactobacillus paragasseri JV-V03

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001146.5	0.53	ribosomal	cyclothiazomycin C	Streptomyces sp. NRRL WC-3908
BGC0001860.4	0.50	ribosomal	thiomuracin	Thermobispora bispora DSM 43833
BGC0000603.5	0.49	ribosomal	cyclothiazomycin	Streptomyces hygroscopicus
BGC0001145.5	0.49	ribosomal	cyclothiazomycin b1	Streptomyces mobaraensis NBRC 13819 = DSM 40847
BGC0001472.5	0.48	ribosomal	berninamycin A	Streptomyces bernensis
BGC0001640.3	0.48	ribosomal	kocurin	Kocuria rosea
BGC0000607.5	0.47	ribosomal	micrococcin P1	Macrococcus caseolyticus
BGC0001174.4	0.47	ribosomal	corynazolicin	Corynebacterium urealyticum DSM 7111
BGC0000613.5	0.47	ribosomal	thiomuracin A	Nonomuraea sp. Bp3714-39
BGC0000606.5	0.47	ribosomal	lactazole	Streptomyces lactacystinaeus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.71 out of 20.47

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.82 out of 18.46

Confidence: medium

Additional weak hits (10):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.93 out of 26.85

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.72 out of 27.78

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 20.05 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.67 out of 26.06

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.34 out of 26.85

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.25 out of 20.97

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.03 out of 19.26

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.61 out of 26.33

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.35 out of 20.01

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.41 out of 24.78

Confidence: weak

(Thi)azol(in)e containing peptide predictions

No core peptides found.

RREFinder

TFBS Finder

Azole RiPPs

RRE predictions

: RRE type: Thiopeptide_F_RRE; Location: 10 - 90; Score: 60.3; RRE type: Heterocycloanthracin_C_RRE; Location: 10 - 94; Score: 39.3
: RRE type: Thiopeptide_F_RRE; Location: 10 - 90; Score: 60.3; RRE type: Heterocycloanthracin_C_RRE; Location: 10 - 94; Score: 39.3

TFBS definitions

Regulator	Description
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

Core peptide predictions

No core peptides found.

NZ_FMHJ01000001.1 - Region 4 - NRPS

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Location: 621,708 - 662,420 nt. (total: 40,713 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase aminotransferase methyltransferase

H181DRAFT_RS02700: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

H181DRAFT_RS02730: DegT/DnrJ/EryC1/StrS aminotransferase

smcogs: SMCOG1056: DegT/DnrJ/EryC1/StrS aminotransferase

H181DRAFT_RS02750: methyltransferase

smcogs: SMCOG1089: methyltransferase

H181DRAFT_RS29735: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: esterase

H181DRAFT_RS02710: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: hydratase

H181DRAFT_RS02740: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase

H181DRAFT_RS02705: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS02725: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.68	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.45 out of 29.72

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 24.85 out of 30.12

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (12):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.08 out of 30.97

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 17.99 out of 26.33

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.79 out of 19.52

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 14.37 out of 27.78

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.13 out of 32.74

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.25 out of 27.78

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.35 out of 36.54

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.86 out of 27.78

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.29 out of 29.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.28 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.07 out of 31.73

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.28 out of 27.18

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 4, location 621707 - 662420:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Thr - D-X) + (NMe-X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

H181DRAFT_RS02705: Thr - X

Search NORINE for peptide: strict or relaxed

AMP-binding (468..882): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LAAHFDFSVWEGSQVLGGEVNMYGITETTVHVTH
Nearest Stachelhaus code(s):: Thr DFWSVGMVHK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1565..1963): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAQAFDASVMEMLIVVGGEVNAYGPTETTCCATT
Nearest Stachelhaus code(s):: Leu DAMLVGAACK (97% 8Å match)
Stachelhaus code match:: 90% (moderate)

H181DRAFT_RS02725: X

AMP-binding (31..441): X

nrpys: Hpa

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: pip (Hpr)

Stachelhaus prediction details:

Stachelhaus sequence:: QATLFTWGCPGGSVQIGSAQQVFGMAEGLLCLTR
Nearest Stachelhaus code(s):: Hpa TWPSQGVLCK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS02750: X

AMP-binding (283..692): X

nrpys: Ph-Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LDSAFDPATFEALYWTGGDTAIYGPTEITLCTTK
Nearest Stachelhaus code(s):: Ph-Gly DPFLWGILCK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
NtrC	Nitrogen regulatory protein C
OsdR	Development and stress management regulator

NZ_FMHJ01000014.1 - Region 1 - NRPS,NRPS-like,T1PKS,linaridin

Download region SVG

Location: 1 - 63,213 nt. (total: 63,213 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
linaridin: (cypemycin or cypI)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

H181DRAFT_RS23005: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

H181DRAFT_RS23065: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: aminotransferase methyltransferase

H181DRAFT_RS22995: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

H181DRAFT_RS23035: methyltransferase

smcogs: SMCOG1248: methyltransferase

Hydrolases: esterase

H181DRAFT_RS22925: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: lyase

H181DRAFT_RS22920: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

H181DRAFT_RS22970: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

H181DRAFT_RS29005: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Isomerases: racemase

H181DRAFT_RS22975: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

Ligases: ligase synthase

H181DRAFT_RS22930: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

H181DRAFT_RS22935: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS23000: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002319.3	1.18	ribosomal	salinipeptin A, salinipeptin B, salinipeptin C, salinipeptin D	Streptomyces sp.
BGC0002135.2	1.18	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	1.17	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	1.16	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002075.3	1.16	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0000307.4	1.16	NRPS	AbT1	Aureobasidium pullulans
BGC0001166.5	1.14	NRPS	HC-toxin	Alternaria jesenskae
BGC0001240.4	1.13	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001249.5	1.13	NRPS	dimethylcoprogen	Alternaria alternata
BGC0000357.5	1.13	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002319.3	0.83	ribosomal	salinipeptin A, salinipeptin B, salinipeptin C, salinipeptin D	Streptomyces sp.
BGC0000583.5	0.80	ribosomal	grisemycin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000582.4	0.77	ribosomal	cypemycin	Streptomyces sp. OH-4156
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.65	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002075.3	0.65	NRPS	pyreudione A, pyreudione B, pyreudione C, pyreudione D, pyreudione E	Pseudomonas fluorescens
BGC0001758.4	0.65	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001050.5	0.64	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.31 out of 18.46

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.10 out of 23.79

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.25 out of 23.79

Confidence: medium

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 24.20 out of 29.51

Confidence: medium

Additional weak hits (17):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.54 out of 26.85

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.19 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.67 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.27 out of 29.38

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 17.22 out of 26.12

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.35 out of 32.74

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.09 out of 21.55

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.93 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.61 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.45 out of 30.98

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.00 out of 32.38

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.97 out of 30.98

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.10 out of 30.98

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.23 out of 30.98

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.66 out of 26.85

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 63213:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (His) + (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 43277:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (His) + (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 20153 - 63213:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (His) + (mal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

H181DRAFT_RS22930: mal

PKS_AT (556..839): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 70.8%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 146.2
Methoxymalonyl-CoA: 73.8
inactive: 59.1
Methylmalonyl-CoA: 54.2
Propionyl-CoA: 39.0
CHC-CoA: 28.0
fatty_acid: 25.4
2-Methylbutyryl-CoA: 25.1
Isobutyryl-CoA: 22.3
trans-1,2-CPDA: 21.4
Benzoyl-CoA: 19.2
Ethylmalonyl-CoA: 15.5
Acetyl-CoA: 15.2
3-Methylbutyryl-CoA: 0.0

H181DRAFT_RS22935: His

Search NORINE for peptide: strict or relaxed

AMP-binding (253..646): His

nrpys: His

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SARSFDSSAAGGLCITAGEVNEYGPTEATVWASF
Nearest Stachelhaus code(s):: His DSALIAEVWK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS23000: X

AMP-binding (13..395): X

nrpys: Ile, Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LPGAWDAFSLEGWLMTGGEVNGYGPVESTVFATT
Nearest Stachelhaus code(s):: Ile or Val DALWMGGVFK (62% 8Å match); Val DALWMGGVFK (53% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
DosR	Hypoxia stress response regulator
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FMHJ01000014.1 - Region 2 - terpene-precursor

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Location: 64,399 - 85,553 nt. (total: 21,155 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

H181DRAFT_RS23195: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

Transferases: methyltransferase

H181DRAFT_RS23120: methyltransferase

smcogs: SMCOG1089: methyltransferase

H181DRAFT_RS23145: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: peptidase

H181DRAFT_RS23110: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

Ligases: synthetase

H181DRAFT_RS23150: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001456.3	0.43	terpene	isorenieratene	Streptomyces argillaceus
BGC0000633.3	0.43	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.43	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002284.3	0.40	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000664.5	0.39	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001810.3	0.39	PKS	trehangelin	Polymorphospora rubra
BGC0000903.3	0.39	other (other)	fluorometabolite	Streptomyces cattleya

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001456.3	0.43	terpene	isorenieratene	Streptomyces argillaceus
BGC0000633.3	0.43	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.43	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000677.5	0.42	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002284.3	0.40	other (other)	corynecin III, corynecin I, corynecin II	Rhodococcus sp. H-CA8f
BGC0000649.3	0.40	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000664.5	0.39	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001810.3	0.39	PKS	trehangelin	Polymorphospora rubra
BGC0000903.3	0.39	other (other)	fluorometabolite	Streptomyces cattleya

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

H181DRAFT_RS23150: PT_FPPS_like

Domain 1 (67 - 377): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (5):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 15.56 out of 21.84

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.28 out of 30.25

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.86 out of 32.93

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description

NZ_FMHJ01000004.1 - Region 1 - other

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Location: 61,163 - 102,695 nt. (total: 41,533 nt) Show pHMM detection rules used

other: (Neocarzinostat or bcpB or frbD or mitE or prnB or CaiA or bacilysin or orf2_PTase)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002573.2	0.57	other (other)	phosphoramidon	Streptomyces mozunensis
BGC0000888.5	0.45	other (other)	bacilysin	Bacillus sp. CS93
BGC0000902.4	0.42	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0001184.4	0.41	other (other)	bacilysin	Bacillus velezensis FZB42
BGC0000285.5	0.40	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0002621.2	0.34	other (other)	angustmycin A, angustmycin B, angustmycin C, aristeromycin, arabinofuranosyladenine	Streptomyces angustmyceticus
BGC0001336.5	0.33	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0000821.5	0.33	other (other)	rebeccamycin	Lechevalieria aerocolonigenes
BGC0002545.3	0.32	PKS	sungeidine C, sungeidine B, sungeidine D, sungeidine H, sungeidine A, sungeidine E, sungeidine F, sungeidine G	Micromonospora sp.
BGC0002471.3	0.32	other (other)	baumannoferrin A, baumannoferrin B	Acinetobacter baumannii AYE

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002573.2	0.57	other (other)	phosphoramidon	Streptomyces mozunensis
BGC0000888.5	0.45	other (other)	bacilysin	Bacillus sp. CS93
BGC0000902.4	0.42	PKS	flaviolin	Saccharopolyspora erythraea NRRL 2338
BGC0001184.4	0.41	other (other)	bacilysin	Bacillus velezensis FZB42
BGC0000285.5	0.40	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0002621.2	0.34	other (other)	angustmycin A, angustmycin B, angustmycin C, aristeromycin, arabinofuranosyladenine	Streptomyces angustmyceticus
BGC0001336.5	0.33	other (other)	erdasporine A, erdasporine B, erdasporine C	uncultured bacterium
BGC0000821.5	0.33	other (other)	rebeccamycin	Lechevalieria aerocolonigenes
BGC0002545.3	0.32	PKS	sungeidine C, sungeidine B, sungeidine D, sungeidine H, sungeidine A, sungeidine E, sungeidine F, sungeidine G	Micromonospora sp.
BGC0002471.3	0.32	other (other)	baumannoferrin A, baumannoferrin B	Acinetobacter baumannii AYE

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.00 out of 20.47

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.33 out of 23.79

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.03 out of 29.72

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.79 out of 23.79

Confidence: medium

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 21.28 out of 26.33

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.90 out of 28.19

Confidence: medium

Additional weak hits (13):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.75 out of 29.38

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.93 out of 39.62

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.53 out of 27.78

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 19.48 out of 27.55

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.76 out of 29.38

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 20.85 out of 32.74

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.80 out of 19.26

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.70 out of 26.33

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.74 out of 29.38

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.54 out of 39.62

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.31 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.74 out of 28.19

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.87 out of 24.78

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
ANR	Anaerobic transcriptional regulator
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C
VqsM	Regulator of quorum-sensing signalling systems
ZuR variant 1	Zinc-responsive repressor

NZ_FMHJ01000004.1 - Region 2 - RiPP-like

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Location: 179,505 - 191,391 nt. (total: 11,887 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: synthase

H181DRAFT_RS08545: cobalamin synthesis protein/P47K family protein

smcogs: SMCOG1178: cobalamin synthesis protein/P47K family protein

H181DRAFT_RS08575: cysteine synthase

smcogs: SMCOG1081: cysteine synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002423.3	0.48	other (other)	arsinothricin	Burkholderia gladioli
BGC0001485.4	0.41	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0000468.5	0.39	ribosomal	bottromycin A2	Streptomyces bottropensis DSM40262
BGC0000470.6	0.39	ribosomal	bottromycin D	Streptomyces sp. WMMB272
BGC0001157.6	0.39	ribosomal	bottromycin A2	Streptomyces scabiei 87.22
BGC0000598.5	0.36	ribosomal	polytheonamide A, polytheonamide B	Candidatus Entotheonella factor
BGC0000469.6	0.36	ribosomal	bottromycin A2	Streptomyces sp. BC16019
BGC0001148.5	0.32	NRPS, ribosomal	pheganomycin	Streptomyces cirratus
BGC0001753.3	0.31	ribosomal	radamycin, globimycin	Streptomyces globisporus subsp. globisporus
BGC0000814.3	0.25	other (other)	K-252a	Nonomuraea longicatena

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002423.3	0.48	other (other)	arsinothricin	Burkholderia gladioli
BGC0001485.4	0.41	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0000468.5	0.39	ribosomal	bottromycin A2	Streptomyces bottropensis DSM40262
BGC0000470.6	0.39	ribosomal	bottromycin D	Streptomyces sp. WMMB272
BGC0001157.6	0.39	ribosomal	bottromycin A2	Streptomyces scabiei 87.22
BGC0000598.5	0.36	ribosomal	polytheonamide A, polytheonamide B	Candidatus Entotheonella factor
BGC0000469.6	0.36	ribosomal	bottromycin A2	Streptomyces sp. BC16019
BGC0001148.5	0.32	NRPS, ribosomal	pheganomycin	Streptomyces cirratus
BGC0001753.3	0.31	ribosomal	radamycin, globimycin	Streptomyces globisporus subsp. globisporus
BGC0000814.3	0.25	other (other)	K-252a	Nonomuraea longicatena

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

NZ_FMHJ01000004.1 - Region 3 - linaridin

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Location: 413,625 - 434,329 nt. (total: 20,705 nt) Show pHMM detection rules used

linaridin: (cypemycin or cypI)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

H181DRAFT_RS09680: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: methyltransferase

H181DRAFT_RS09690: methyltransferase

smcogs: SMCOG1248: methyltransferase

Hydrolases: hydrolase

H181DRAFT_RS09700: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001735.5	0.53	other (other)	pentostatine, vidarabine	Streptomyces antibioticus
BGC0001188.3	0.49	ribosomal	legonaridin	Streptomyces sp. CT34
BGC0001226.4	0.43	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.42	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0002669.2	0.42	ribosomal	TVA-YJ-2	Streptomyces sp. NRRL S-87
BGC0002698.2	0.42	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001660.3	0.41	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0000501.3	0.41	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000513.3	0.41	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0001579.4	0.40	ribosomal	duramycin	Streptomyces cinnamoneus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001735.5	0.53	other (other)	pentostatine, vidarabine	Streptomyces antibioticus
BGC0001188.3	0.49	ribosomal	legonaridin	Streptomyces sp. CT34
BGC0001226.4	0.43	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.42	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0002669.2	0.42	ribosomal	TVA-YJ-2	Streptomyces sp. NRRL S-87
BGC0002698.2	0.42	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001660.3	0.41	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0000501.3	0.41	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000513.3	0.41	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0001579.4	0.40	ribosomal	duramycin	Streptomyces cinnamoneus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 17.48 out of 18.57

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.76 out of 23.79

Confidence: medium

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 21.73 out of 27.04

Confidence: medium

Additional weak hits (12):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.91 out of 39.62

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.96 out of 20.97

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.68 out of 26.06

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.68 out of 30.25

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.79 out of 31.73

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 20.06 out of 30.25

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.20 out of 30.25

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.91 out of 29.65

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.37 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.63 out of 30.98

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.65 out of 28.19

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.45 out of 30.98

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
DasR	N-acetylglucosamine dependent repressor
IolR	Regulator of the inositol catabolism
ToxT	Regulator of MSHA biosynthesis

NZ_FMHJ01000003.1 - Region 1 - NRPS,NRPS-like

Download region SVG

Location: 75,655 - 141,826 nt. (total: 66,172 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase electron transfer

H181DRAFT_RS05815: flavodoxin

smcogs: SMCOG1134: flavodoxin

H181DRAFT_RS05880: 3-hydroxybutyryl-CoA dehydrogenase

smcogs: SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase

H181DRAFT_RS05900: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Hydrolases: phosphatase

H181DRAFT_RS05895: FkbH like protein

smcogs: SMCOG1256: FkbH like protein

Isomerases: mutase

H181DRAFT_RS05945: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase synthase

H181DRAFT_RS05870: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS05875: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS05885: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

H181DRAFT_RS05890: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS05910: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.82	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002164.2	0.66	NRPS	peramine	Epichloe festucae
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000342.4	0.65	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.65	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001135.5	0.68	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 23.74 out of 29.72

Confidence: medium

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 26.50 out of 30.12

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 21.35 out of 23.79

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.09 out of 23.79

Confidence: medium

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 25.46 out of 32.38

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.33 out of 19.52

Confidence: strong

Additional weak hits (5):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.56 out of 39.62

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 21.53 out of 32.38

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 20.97 out of 32.93

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 75654 - 141826:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (mal) + (D-Ala) + (Thr - D-Val)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 75654 - 132915:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (mal) + (D-Ala) + (Thr - D-Val)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 99843 - 141826:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X) + (mal) + (D-Ala) + (Thr - D-Val)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

H181DRAFT_RS05870: Thr - Val

Search NORINE for peptide: strict or relaxed

AMP-binding (513..934): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: LEAHFDFSVWEGNQVFGGEVNMYGITETTVHASR
Nearest Stachelhaus code(s):: Thr DFWNVGMVHK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1661..2077): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LDGSFDASTFEGWLWTGGDVNGYGPTETTTFATR
Nearest Stachelhaus code(s):: Val DAYWWGGTFK (91% 8Å match)
Stachelhaus code match:: 90% (moderate)

H181DRAFT_RS05875: Ala

Search NORINE for peptide: strict or relaxed

AMP-binding (478..903): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: LWATFDVSVAEPCASMAGEMNVYGPTEATVYATA
Nearest Stachelhaus code(s):: Ala DVFSVAVVYK (76% 8Å match)
Stachelhaus code match:: 70% (weak)

H181DRAFT_RS05890: X

AMP-binding (28..443): X

nrpys: Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: SPRRFDGMLGGWIVVVAGEVNEYGPTETTISCTV
Nearest Stachelhaus code(s):: Trp DVGIVGEITK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CsoR	Copper-responsive repressor
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C
OsdR	Development and stress management regulator

NZ_FMHJ01000003.1 - Region 2 - lanthipeptide-class-ii

BGC0001229.4 (SBI_06989):

Download region SVG

Location: 490,570 - 518,636 nt. (total: 28,067 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

H181DRAFT_RS07525: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Lyases: cyclase

H181DRAFT_RS07530: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

H181DRAFT_RS07540: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001229.4	0.81	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0002110.3	0.76	ribosomal	birimositide	Streptomyces rimosus subsp. rimosus WC-3908
BGC0000520.5	0.59	ribosomal	lacticin 3147 A1, lacticin 3147 A2	Lactococcus lactis
BGC0000555.3	0.58	ribosomal	staphylococcin C55 α, staphylococcin C55 β	Staphylococcus aureus
BGC0000623.3	0.53	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0001311.5	0.53	ribosomal	flavecins	Ruminococcus flavefaciens FD-1
BGC0000525.5	0.53	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002663.2	0.52	ribosomal	amyloliquecidin GF610	Bacillus amyloliquefaciens UMAF6639
BGC0000909.3	0.51	other (other)	melanin	Streptomyces avermitilis
BGC0001391.3	0.51	ribosomal	thusin	Bacillus thuringiensis serovar rongseni

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001229.4	0.81	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0002110.3	0.76	ribosomal	birimositide	Streptomyces rimosus subsp. rimosus WC-3908
BGC0000520.5	0.59	ribosomal	lacticin 3147 A1, lacticin 3147 A2	Lactococcus lactis
BGC0000555.3	0.58	ribosomal	staphylococcin C55 α, staphylococcin C55 β	Staphylococcus aureus
BGC0000623.3	0.53	ribosomal	plantaricin W α, plantaricin W β	Lactobacillus plantarum
BGC0001311.5	0.53	ribosomal	flavecins	Ruminococcus flavefaciens FD-1
BGC0000525.5	0.53	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002663.2	0.52	ribosomal	amyloliquecidin GF610	Bacillus amyloliquefaciens UMAF6639
BGC0000909.3	0.51	other (other)	melanin	Streptomyces avermitilis
BGC0001391.3	0.51	ribosomal	thusin	Bacillus thuringiensis serovar rongseni

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for H181DRAFT_RS07530

H181DRAFT_RS29345

Leader:

MSDDILGG

Core:

YDEAELVELSDADAYGGTAWVCATVTLATAAICPTTKCTSQC

Core with dehydrations:

YDEAELVELDhaDADAYGGDhbAWVCysADhbVDhbLADhbAAICysPDhbDhbKCysDhbDhaQCys

antiSMASH-DB 4.0 matches for H181DRAFT_RS29345

100.0%

NZ_BMMP01000005.1and 3 others:

NZ_BMMP01000005.1 Lanthipeptides: IEY56_RS09415_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS29345_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS29345_lanthipeptide

Lanthipeptides: IEY56_RS09415_lanthipeptide

1

YDEAELVELSDADAYGGTAWVCATVTLATAAICPTTKCTSQC

42

YDEAELVELSDADAYGGTAWVCATVTLATAAICPTTKCTSQC

1

YDEAELVELSDADAYGGTAWVCATVTLATAAICPTTKCTSQC

42

88.4%

NZ_QLLX01000002.1and 1 other:

NZ_QLLX01000002.1 Lanthipeptides: K378_RS30040_lanthipeptide

Lanthipeptides: K378_RS30040_lanthipeptide

1

YDEAELVELSDADAYGGTA-WVCATVTLATAAICPTTKCTSQC

42

YDEAELVELSDAD YGGT W ATVTLATAAICPTTKCTSQC

1

YDEAELVELSDADTYGGTGPWCIATVTLATAAICPTTKCTSQC

43

86.0%

NZ_JAASAG010000001.1and 1 other:

NZ_JAASAG010000001.1 Lanthipeptides: HCC61_RS01465_lanthipeptide

Lanthipeptides: HCC61_RS01465_lanthipeptide

1

YDEAELVELSDADAYGGTA-WVCATVTLATAAICPTTKCTSQC

42

YDE ELVELSDAD YGGT W ATVTLATAAICPTTKCTSQC

1

YDEDELVELSDADTYGGTGPWCVATVTLATAAICPTTKCTSQC

43

with 104 more sequence matches

Only the first 10 are displayed here.

76.7%

NZ_CP107567.1and 3 others:

NZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptide

Lanthipeptides: OGH68_RS19755_lanthipeptide

1

YDEAELVELSDADAYGGTA-WVCATVTLATAAICPTTKCTSQC

42

YDEAEL+ELS+AD YGGTA W ATVTL +AA+CPTTKCTS C

1

YDEAELIELSEADTYGGTAAWCIATVTLVSAAMCPTTKCTSAC

43

72.1%

NZ_GG657757.1and 1 other:

NZ_GG657757.1 Lanthipeptides: SSQG_RS41860_lanthipeptide

Lanthipeptides: SSQG_RS41860_lanthipeptide

1

YDEAELVELSDADAYGGTA-WVCATVTLATAAICPTTKCTSQC

42

YDEAEL+ELS+AD +GGT W ATVTL TA +CPTTKCTS+C

1

YDEAELIELSEADNHGGTGPWCIATVTLVTATVCPTTKCTSKC

43

69.8%

NZ_KL997439.1and 1 other:

NZ_KL997439.1 Lanthipeptides: IQ62_RS33140_lanthipeptide

Lanthipeptides: IQ62_RS33140_lanthipeptide

1

YDEAELVELSDADAYGGTA-WVCATVTLATAAICPTTKCTSQC

42

YDEAEL+ELS+AD +GGT W ATVTL +A CPTTKCTS C

1

YDEAELIELSEADTHGGTGPWCIATVTLVSAVACPTTKCTSSC

43

65.2%

NZ_AKXV01000031.1and 1 other:

NZ_AKXV01000031.1 Lanthipeptides: Y19_RS44045_lanthipeptide

Lanthipeptides: Y19_RS44045_lanthipeptide

1

YDEAELVELSDADAYGGTAWVCATV----TLATAAICPTTKCTSQC

42

YDE ELVE+ +AD YGGT W CA V TL +A+ CPTTKCTSQC

1

YDEFELVEMGEADVYGGTTWSCAIVTAVATLVSASACPTTKCTSQC

46

65.1%

NZ_BNBV01000031.1and 3 others:

NZ_BNBV01000031.1 Lanthipeptides: IE286_RS22985_lanthipeptideNZ_BNBV01000031.1 Lanthipeptides: IE286_RS22990_lanthipeptideNZ_BNBV01000031.1 Lanthipeptides: IE286_RS22985_lanthipeptide

Lanthipeptides: IE286_RS22990_lanthipeptide

1

YDEAELVELSDADAYGGTAWVCATVTL-ATAAICPTTKCTSQC

42

YDEAEL+ELS+AD GGT V VT+ A+AA CPTTKCT+ C

1

YDEAELIELSEADVTGGTTPVSIAVTVAASAAFCPTTKCTNDC

43

62.8%

NZ_BJTV01000004.1and 1 other:

NZ_BJTV01000004.1 Lanthipeptides: TNCT1_RS35805_lanthipeptide

Lanthipeptides: TNCT1_RS35805_lanthipeptide

1

YDEAELVELSDADAYGGTAWVCATVTL-ATAAICPTTKCTSQC

42

YDEAEL+ELS+AD GGT V VT+ A+ A CPT KCTS C

1

YDEAELIELSEADVTGGTTPVSIAVTVAASVAFCPTIKCTSAC

43

58.1%

NZ_CP016795.1and 7 others:

NZ_CP016795.1 Lanthipeptides: BC342_RS37680_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18955_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18955_lanthipeptideNZ_CP043317.1 Lanthipeptides: FYC74_RS17685_lanthipeptideNZ_CP043317.1 Lanthipeptides: FYC74_RS17685_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01490_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01490_lanthipeptide

Lanthipeptides: BC342_RS37680_lanthipeptide

1

YDEAELVELSDADAYGG-TAWVCATVTLATAAICPTTKCTSQC

42

YDEAEL+ELS+AD GG T A A+ A CPTTKCT +C

1

YDEAELIELSEADVTGGTTPVSVAVSVAASVAFCPTTKCTKEC

43

54.5%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: IE314_RS15870_lanthipeptide

Lanthipeptides: IE314_RS15870_lanthipeptide

1

YDEAELVELSDADAYGGT-AWVCATVTLATA-AICPTTKCTSQC

42

YDE EL+EL + A+GGT + VC ++A + A CPTTKCTS+C

1

YDELELIELGEDVAHGGTTSPVCIATSVAVSTAFCPTTKCTSKC

44

54.3%

NZ_JAJAGO010000028.1and 1 other:

NZ_JAJAGO010000028.1 Lanthipeptides: LHJ74_RS33595_lanthipeptide

Lanthipeptides: LHJ74_RS33595_lanthipeptide

1

YDEAELVELSDADAYGGT------AWVCATVTLATAAICPTTKCTSQC

42

YDEAEL+EL+D++A+GGT +W C +T AT +ICPT+ C+S+C

1

YDEAELIELADSEAFGGTSPATTSSWTC--ITAATISICPTSACSSKC

46

48.1%

NC_016582.1and 1 other:

NC_016582.1 Lanthipeptides: SBI_RS34370_lanthipeptide

Lanthipeptides: SBI_RS34370_lanthipeptide

1

YDEAELVELSDADAYGGTAWVCATVTLA------------TAAICPTTKCTSQC

42

+DEAELVELS+AD +GGT + L +AA CPTTKCTS+C

1

WDEAELVELSEADVHGGTDPAVTGLILGIVAIQAGVAVGVSAAFCPTTKCTSRC

54

MIBiG 4.0 matches for H181DRAFT_RS29345

26.2%

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

29

TAAICPTTKCTSQC

42

+AA CPTTKCTS+C

24

SAAFCPTTKCTSRC

37

21.4%

BGC0002110.3 (QKV49894.1):

Lanthipeptide: birimositide

32

ICPTTKCTSQC

42

CPTTKCTS+C

16

FCPTTKCTSKC

26

H181DRAFT_RS07545

Leader:

MYKGDVSLVEEIAEQDFDGVAYGA

Core:

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

Core with dehydrations:

CysDhbDhbNDhbFDhaLDhaDVLGNDhbGGWCysDhbLDhbVECysQPNCysN

antiSMASH-DB 4.0 matches for H181DRAFT_RS07545

100.0%

NZ_BMMP01000005.1and 3 others:

NZ_BMMP01000005.1 Lanthipeptides: IEY56_RS28370_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS07545_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS07545_lanthipeptide

Lanthipeptides: IEY56_RS28370_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

93.3%

NZ_JAASAG010000001.1and 1 other:

NZ_JAASAG010000001.1 Lanthipeptides: HCC61_RS30395_lanthipeptide

Lanthipeptides: HCC61_RS30395_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD L NTGGWCTLTVECQPNCN

1

CTTNTFSLSDALTNTGGWCTLTVECQPNCN

30

90.0%

NZ_BNBV01000031.1and 1 other:

NZ_BNBV01000031.1 Lanthipeptides: IE286_RS22995_lanthipeptide

Lanthipeptides: IE286_RS22995_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD LGN+GGWCTLT ECQPNCN

1

CTTNTFSLSDALGNSGGWCTLTKECQPNCN

30

with 105 more sequence matches

Only the first 10 are displayed here.

90.0%

NZ_CP107567.1and 3 others:

NZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptide

Lanthipeptides: OGH68_RS19760_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD LGN GGWCTLT ECQPNCN

1

CTTNTFSLSDWLGNQGGWCTLTKECQPNCN

30

90.0%

NZ_QLLX01000002.1and 1 other:

NZ_QLLX01000002.1 Lanthipeptides: K378_RS05175_lanthipeptide

Lanthipeptides: K378_RS05175_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD L N GGWCTLTVECQPNCN

1

CTTNTFSLSDALTNQGGWCTLTVECQPNCN

30

86.7%

NZ_AKXV01000031.1and 4 others:

NZ_AKXV01000031.1 Lanthipeptides: Y19_RS0109895_lanthipeptideNZ_JABBGE010000004.1 Lanthipeptides: HHL19_RS13545_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS06140_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS06140_lanthipeptide

Lanthipeptides: Y19_RS0109895_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

C+TNTFSLSD LGN GGWCTLT ECQPNCN

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

86.7%

NZ_BJTV01000004.1and 1 other:

NZ_BJTV01000004.1 Lanthipeptides: TNCT1_RS10200_lanthipeptide

Lanthipeptides: TNCT1_RS10200_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD LGN GGWCTLT ECQ NCN

1

CTTNTFSLSDALGNHGGWCTLTKECQANCN

30

86.7%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: IE314_RS15865_lanthipeptide

Lanthipeptides: IE314_RS15865_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD +GN GGWCTLT ECQPNCN

1

CTTNTFSLSDAVGNHGGWCTLTKECQPNCN

30

86.7%

NZ_CP016795.1and 5 others:

NZ_CP016795.1 Lanthipeptides: BC342_RS18360_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18960_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18960_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01495_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01495_lanthipeptide

Lanthipeptides: BC342_RS18360_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD LGN GGWCTLT ECQPNC+

1

CTTNTFSLSDALGNHGGWCTLTKECQPNCS

30

86.7%

NZ_CP043317.1and 1 other:

NZ_CP043317.1 Lanthipeptides: FYC74_RS17680_lanthipeptide

Lanthipeptides: FYC74_RS17680_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD LGN GGWCTLT ECQPNC+

1

CTTNTFSLSDSLGNHGGWCTLTKECQPNCS

30

86.7%

NZ_CP047419.1and 3 others:

NZ_CP047419.1 Lanthipeptides: GTU71_RS12805_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptide

Lanthipeptides: GTU71_RS12805_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTF+LS+VLGN GGWCTLTVECQPNC

1

CTTNTFTLSNVLGNQGGWCTLTVECQPNCK

30

80.0%

NZ_LT985747.1and 1 other:

NZ_LT985747.1 Lanthipeptides: C8268_RS00165_lanthipeptide

Lanthipeptides: C8268_RS00165_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTF+LSD LGN GGWCTLT ECQ +CN

1

CTTNTFTLSDYLGNKGGWCTLTKECQRSCN

30

76.7%

NZ_CP084530.1and 1 other:

NZ_CP084530.1 Lanthipeptides: LDO05_RS02075_lanthipeptide

Lanthipeptides: LDO05_RS02075_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTF+LS+ LGN GGWCTLT ECQ +CN

1

CTTNTFTLSNYLGNNGGWCTLTKECQRSCN

30

MIBiG 4.0 matches for H181DRAFT_RS07545

86.7%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLS+ LGN GGWCTLT ECQPNCN

1

CTTNTFSLSNALGNNGGWCTLTKECQPNCN

30

50.0%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

6

FSLSDVLGNTGGWCTLTVECQPNCN

30

+++S LGN G +CTLTVEC P+CN

4

YNISCRLGNKGAYCTLTVECMPSCN

28

25.6%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

19

CTLTVECQPNCN

30

CTL+ ECQPNCN

28

CTLSKECQPNCN

39

allorf_493830_493922

Leader:

MDFPSGRDGHDIGGHSVTGG

Core:

RGRSCLAAQH

Core with dehydrations:

RGRDhaCysLAAQH

antiSMASH-DB 4.0 matches for allorf_493830_493922

100.0%

NZ_FMHJ01000003.1:

Lanthipeptides: allorf_493830_493922_lanthipeptide

1

RGRSCLAAQH

10

RGRSCLAAQH

1

RGRSCLAAQH

10

allorf_498973_499089

Leader:

MRACLRVTVAWSRIGGPVAGA

Core:

VCFTSATPCGGSVKRSR

Core with dehydrations:

VCysFDhbDhaADhbPCysGGDhaVKRDhaR

antiSMASH-DB 4.0 matches for allorf_498973_499089

100.0%

NZ_BMMP01000005.1and 3 others:

NZ_BMMP01000005.1 Lanthipeptides: allorf_174812_174928_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: allorf_498973_499089_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: allorf_498973_499089_lanthipeptide

Lanthipeptides: allorf_174812_174928_lanthipeptide

1

VCFTSATPCGGSVKRSR

17

VCFTSATPCGGSVKRSR

1

VCFTSATPCGGSVKRSR

17

allorf_511535_511666

Leader:

MKAAERISESAA

Core:

CWRRTSSHGLHVRGTVSRDVPILTIPGDLYD

Core with dehydrations:

CysWRRDhbDhaDhaHGLHVRGDhbVDhaRDVPILDhbIPGDLYD

antiSMASH-DB 4.0 matches for allorf_511535_511666

100.0%

NZ_FMHJ01000003.1and 1 other:

NZ_FMHJ01000003.1 Lanthipeptides: allorf_511535_511666_lanthipeptide

Lanthipeptides: allorf_511535_511666_lanthipeptide

1

CWRRTSSHGLHVRGTVSRDVPILTIPGDLYD

31

CWRRTSSHGLHVRGTVSRDVPILTIPGDLYD

1

CWRRTSSHGLHVRGTVSRDVPILTIPGDLYD

31

78.8%

NZ_BMMP01000005.1and 1 other:

NZ_BMMP01000005.1 Lanthipeptides: allorf_162219_162356_lanthipeptide

Lanthipeptides: allorf_162219_162356_lanthipeptide

1

CWRRTSSHGLHVRGTVSRDVPILTIPGDLYD

31

CWRRTSSHGLHVRGT S+DVPILT+PGD++D

1

CWRRTSSHGLHVRGTDSQDVPILTVPGDMHD

31

25.8%

NZ_CP022203.1:

Lanthipeptides: allorf_3832712_3832861_lanthipeptide

16

VSRD-VPILTIPGDLYD

31

++RD VP++ +PG L D

3

LTRDTVPVVVVPGCLSD

19

with 1 more sequence matches

22.6%

NZ_CP071868.1:

Lassopeptides: J4E96_RS19650_lassopeptide

13

RGTVSRDVPILTIP

26

RG + R+ P LT+P

3

RGVLEREAPSLTLP

16

allorf_511619_511831

Leader:

MAPSWARRRAVRVICSAVRVRAPGA

Core:

DGARPPGERAGLCADGQGRTGSPTEPVVPRSVVQIAWNRQDRHVP

Core with dehydrations:

DGARPPGERAGLCysADGQGRDhbGDhaPDhbEPVVPRDhaVVQIAWNRQDRHVP

antiSMASH-DB 4.0 matches for allorf_511619_511831

100.0%

NZ_FMHJ01000003.1and 1 other:

NZ_FMHJ01000003.1 Lanthipeptides: allorf_511619_511831_lanthipeptide

Lanthipeptides: allorf_511619_511831_lanthipeptide

1

DGARPPGERAGLCADGQGRTGSPTEPVVPRSVVQIAWNRQDRHVP

45

DGARPPGERAGLCADGQGRTGSPTEPVVPRSVVQIAWNRQDRHVP

1

DGARPPGERAGLCADGQGRTGSPTEPVVPRSVVQIAWNRQDRHVP

45

23.7%

NZ_CP031058.1:

Lanthipeptides: allorf_3313863_3314099_lanthipeptide

6

PGERAGLCADGQGRTGSPTEPVVP------RSVVQIAWNRQDRHV

44

P RA CA G+ R G +P R I W RH+

4

PCSRALSCALGKTRVGLLAQPCRAHAPNPWRQRSWIGWPAASRHI

48

22.2%

NC_012489.1:

Lanthipeptides: allorf_4594448_4594576_lanthipeptide

2

GARPPGERAGLCADGQGRTGSPTEP

26

G RPP G C T PT P

8

GCRPPSPEHGGCPTMPSDTALPTRP

32

with 4 more sequence matches

20.0%

NZ_CP110813.1:

Lanthipeptides: allorf_5686197_5686325_lanthipeptide

18

GRTGSPTEPVVPRSVVQIAWNRQDRHV

44

GRT P PRS+V+++ + R +

1

GRTFVPKPETRPRSIVRLSTHPMTRRI

27

15.6%

NC_007622.1and 9 others:

NC_017337.1 Lanthipeptides: SAOV_RS09490_lanthipeptideNZ_CP038268.1 Lanthipeptides: SaO55_RS09725_lanthipeptideNZ_CP047792.1 Lanthipeptides: E3S88_RS07350_lanthipeptideNZ_CP094743.1 Lanthipeptides: MUA62_RS09295_lanthipeptideNZ_CP102962.1 Lanthipeptides: NW971_RS09560_lanthipeptideNZ_GG730342.1 Lanthipeptides: SAUG_RS17380_lanthipeptideNZ_JAGSAY010000004.1 Lanthipeptides: KC186_RS06750_lanthipeptideNZ_LR133917.1 Lanthipeptides: ELZ45_RS09165_lanthipeptideNZ_LS483317.1 Lanthipeptides: DQL75_RS09705_lanthipeptide

Lanthipeptides: SAB_RS08995_lanthipeptide

12

LCADGQGRTGS

22

LC+ G G+TGS

7

LCSFGCGKTGS

17

15.6%

NZ_KV802313.1and 1 other:

NZ_KV817389.1 Lanthipeptides: allorf_50141_50287_lanthipeptide

Lanthipeptides: allorf_165895_166041_lanthipeptide

17

QGRTGSPTEPVVPRSVV

33

QG +GS T P R +

7

QGSSGSLTSPASERYCI

23

15.6%

NZ_LR134304.1:

Lanthipeptides: EL116_RS09250_lanthipeptide

12

LCADGQGRTGS

22

LC+ G G TGS

7

LCSYGCGNTGS

17

MIBiG 4.0 matches for allorf_511619_511831

11.1%

BGC0000582.4 (ADR72962.1):

Linaridin: cypemycin

21

GSPTEPVVPRSVVQ

34

+P P V + V+Q

1

ATPATPTVAQFVIQ

14

Lanthipeptide(s) for H181DRAFT_RS07540

H181DRAFT_RS29345

Leader:

MSDDILGG

Core:

YDEAELVELSDADAYGGTAWVCATVTLATAAICPTTKCTSQC

Core with dehydrations:

YDEAELVELDhaDADAYGGDhbAWVCysADhbVDhbLADhbAAICysPDhbDhbKCysDhbDhaQCys

antiSMASH-DB 4.0 matches for H181DRAFT_RS29345

100.0%

NZ_BMMP01000005.1and 3 others:

NZ_BMMP01000005.1 Lanthipeptides: IEY56_RS09415_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS29345_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS29345_lanthipeptide

Lanthipeptides: IEY56_RS09415_lanthipeptide

1

YDEAELVELSDADAYGGTAWVCATVTLATAAICPTTKCTSQC

42

YDEAELVELSDADAYGGTAWVCATVTLATAAICPTTKCTSQC

1

YDEAELVELSDADAYGGTAWVCATVTLATAAICPTTKCTSQC

42

88.4%

NZ_QLLX01000002.1and 1 other:

NZ_QLLX01000002.1 Lanthipeptides: K378_RS30040_lanthipeptide

Lanthipeptides: K378_RS30040_lanthipeptide

1

YDEAELVELSDADAYGGTA-WVCATVTLATAAICPTTKCTSQC

42

YDEAELVELSDAD YGGT W ATVTLATAAICPTTKCTSQC

1

YDEAELVELSDADTYGGTGPWCIATVTLATAAICPTTKCTSQC

43

86.0%

NZ_JAASAG010000001.1and 1 other:

NZ_JAASAG010000001.1 Lanthipeptides: HCC61_RS01465_lanthipeptide

Lanthipeptides: HCC61_RS01465_lanthipeptide

1

YDEAELVELSDADAYGGTA-WVCATVTLATAAICPTTKCTSQC

42

YDE ELVELSDAD YGGT W ATVTLATAAICPTTKCTSQC

1

YDEDELVELSDADTYGGTGPWCVATVTLATAAICPTTKCTSQC

43

with 104 more sequence matches

Only the first 10 are displayed here.

76.7%

NZ_CP107567.1and 3 others:

NZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19755_lanthipeptide

Lanthipeptides: OGH68_RS19755_lanthipeptide

1

YDEAELVELSDADAYGGTA-WVCATVTLATAAICPTTKCTSQC

42

YDEAEL+ELS+AD YGGTA W ATVTL +AA+CPTTKCTS C

1

YDEAELIELSEADTYGGTAAWCIATVTLVSAAMCPTTKCTSAC

43

72.1%

NZ_GG657757.1and 1 other:

NZ_GG657757.1 Lanthipeptides: SSQG_RS41860_lanthipeptide

Lanthipeptides: SSQG_RS41860_lanthipeptide

1

YDEAELVELSDADAYGGTA-WVCATVTLATAAICPTTKCTSQC

42

YDEAEL+ELS+AD +GGT W ATVTL TA +CPTTKCTS+C

1

YDEAELIELSEADNHGGTGPWCIATVTLVTATVCPTTKCTSKC

43

69.8%

NZ_KL997439.1and 1 other:

NZ_KL997439.1 Lanthipeptides: IQ62_RS33140_lanthipeptide

Lanthipeptides: IQ62_RS33140_lanthipeptide

1

YDEAELVELSDADAYGGTA-WVCATVTLATAAICPTTKCTSQC

42

YDEAEL+ELS+AD +GGT W ATVTL +A CPTTKCTS C

1

YDEAELIELSEADTHGGTGPWCIATVTLVSAVACPTTKCTSSC

43

65.2%

NZ_AKXV01000031.1and 1 other:

NZ_AKXV01000031.1 Lanthipeptides: Y19_RS44045_lanthipeptide

Lanthipeptides: Y19_RS44045_lanthipeptide

1

YDEAELVELSDADAYGGTAWVCATV----TLATAAICPTTKCTSQC

42

YDE ELVE+ +AD YGGT W CA V TL +A+ CPTTKCTSQC

1

YDEFELVEMGEADVYGGTTWSCAIVTAVATLVSASACPTTKCTSQC

46

65.1%

NZ_BNBV01000031.1and 3 others:

NZ_BNBV01000031.1 Lanthipeptides: IE286_RS22985_lanthipeptideNZ_BNBV01000031.1 Lanthipeptides: IE286_RS22990_lanthipeptideNZ_BNBV01000031.1 Lanthipeptides: IE286_RS22985_lanthipeptide

Lanthipeptides: IE286_RS22990_lanthipeptide

1

YDEAELVELSDADAYGGTAWVCATVTL-ATAAICPTTKCTSQC

42

YDEAEL+ELS+AD GGT V VT+ A+AA CPTTKCT+ C

1

YDEAELIELSEADVTGGTTPVSIAVTVAASAAFCPTTKCTNDC

43

62.8%

NZ_BJTV01000004.1and 1 other:

NZ_BJTV01000004.1 Lanthipeptides: TNCT1_RS35805_lanthipeptide

Lanthipeptides: TNCT1_RS35805_lanthipeptide

1

YDEAELVELSDADAYGGTAWVCATVTL-ATAAICPTTKCTSQC

42

YDEAEL+ELS+AD GGT V VT+ A+ A CPT KCTS C

1

YDEAELIELSEADVTGGTTPVSIAVTVAASVAFCPTIKCTSAC

43

58.1%

NZ_CP016795.1and 7 others:

NZ_CP016795.1 Lanthipeptides: BC342_RS37680_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18955_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18955_lanthipeptideNZ_CP043317.1 Lanthipeptides: FYC74_RS17685_lanthipeptideNZ_CP043317.1 Lanthipeptides: FYC74_RS17685_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01490_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01490_lanthipeptide

Lanthipeptides: BC342_RS37680_lanthipeptide

1

YDEAELVELSDADAYGG-TAWVCATVTLATAAICPTTKCTSQC

42

YDEAEL+ELS+AD GG T A A+ A CPTTKCT +C

1

YDEAELIELSEADVTGGTTPVSVAVSVAASVAFCPTTKCTKEC

43

54.5%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: IE314_RS15870_lanthipeptide

Lanthipeptides: IE314_RS15870_lanthipeptide

1

YDEAELVELSDADAYGGT-AWVCATVTLATA-AICPTTKCTSQC

42

YDE EL+EL + A+GGT + VC ++A + A CPTTKCTS+C

1

YDELELIELGEDVAHGGTTSPVCIATSVAVSTAFCPTTKCTSKC

44

54.3%

NZ_JAJAGO010000028.1and 1 other:

NZ_JAJAGO010000028.1 Lanthipeptides: LHJ74_RS33595_lanthipeptide

Lanthipeptides: LHJ74_RS33595_lanthipeptide

1

YDEAELVELSDADAYGGT------AWVCATVTLATAAICPTTKCTSQC

42

YDEAEL+EL+D++A+GGT +W C +T AT +ICPT+ C+S+C

1

YDEAELIELADSEAFGGTSPATTSSWTC--ITAATISICPTSACSSKC

46

48.1%

NC_016582.1and 1 other:

NC_016582.1 Lanthipeptides: SBI_RS34370_lanthipeptide

Lanthipeptides: SBI_RS34370_lanthipeptide

1

YDEAELVELSDADAYGGTAWVCATVTLA------------TAAICPTTKCTSQC

42

+DEAELVELS+AD +GGT + L +AA CPTTKCTS+C

1

WDEAELVELSEADVHGGTDPAVTGLILGIVAIQAGVAVGVSAAFCPTTKCTSRC

54

MIBiG 4.0 matches for H181DRAFT_RS29345

26.2%

BGC0001229.4 (SBI_06989):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

29

TAAICPTTKCTSQC

42

+AA CPTTKCTS+C

24

SAAFCPTTKCTSRC

37

21.4%

BGC0002110.3 (QKV49894.1):

Lanthipeptide: birimositide

32

ICPTTKCTSQC

42

CPTTKCTS+C

16

FCPTTKCTSKC

26

H181DRAFT_RS07545

Leader:

MYKGDVSLVEEIAEQDFDGVAYGA

Core:

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

Core with dehydrations:

CysDhbDhbNDhbFDhaLDhaDVLGNDhbGGWCysDhbLDhbVECysQPNCysN

antiSMASH-DB 4.0 matches for H181DRAFT_RS07545

100.0%

NZ_BMMP01000005.1and 3 others:

NZ_BMMP01000005.1 Lanthipeptides: IEY56_RS28370_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS07545_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: H181DRAFT_RS07545_lanthipeptide

Lanthipeptides: IEY56_RS28370_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

93.3%

NZ_JAASAG010000001.1and 1 other:

NZ_JAASAG010000001.1 Lanthipeptides: HCC61_RS30395_lanthipeptide

Lanthipeptides: HCC61_RS30395_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD L NTGGWCTLTVECQPNCN

1

CTTNTFSLSDALTNTGGWCTLTVECQPNCN

30

90.0%

NZ_BNBV01000031.1and 1 other:

NZ_BNBV01000031.1 Lanthipeptides: IE286_RS22995_lanthipeptide

Lanthipeptides: IE286_RS22995_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD LGN+GGWCTLT ECQPNCN

1

CTTNTFSLSDALGNSGGWCTLTKECQPNCN

30

with 105 more sequence matches

Only the first 10 are displayed here.

90.0%

NZ_CP107567.1and 3 others:

NZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptideNZ_CP107567.1 Lanthipeptides: OGH68_RS19760_lanthipeptide

Lanthipeptides: OGH68_RS19760_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD LGN GGWCTLT ECQPNCN

1

CTTNTFSLSDWLGNQGGWCTLTKECQPNCN

30

90.0%

NZ_QLLX01000002.1and 1 other:

NZ_QLLX01000002.1 Lanthipeptides: K378_RS05175_lanthipeptide

Lanthipeptides: K378_RS05175_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD L N GGWCTLTVECQPNCN

1

CTTNTFSLSDALTNQGGWCTLTVECQPNCN

30

86.7%

NZ_AKXV01000031.1and 4 others:

NZ_AKXV01000031.1 Lanthipeptides: Y19_RS0109895_lanthipeptideNZ_JABBGE010000004.1 Lanthipeptides: HHL19_RS13545_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS06140_lanthipeptideNZ_LMEI01000006.1 Lanthipeptides: ASC82_RS06140_lanthipeptide

Lanthipeptides: Y19_RS0109895_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

C+TNTFSLSD LGN GGWCTLT ECQPNCN

1

CSTNTFSLSDFLGNQGGWCTLTKECQPNCN

30

86.7%

NZ_BJTV01000004.1and 1 other:

NZ_BJTV01000004.1 Lanthipeptides: TNCT1_RS10200_lanthipeptide

Lanthipeptides: TNCT1_RS10200_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD LGN GGWCTLT ECQ NCN

1

CTTNTFSLSDALGNHGGWCTLTKECQANCN

30

86.7%

NZ_BNCA01000011.1and 1 other:

NZ_BNCA01000011.1 Lanthipeptides: IE314_RS15865_lanthipeptide

Lanthipeptides: IE314_RS15865_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD +GN GGWCTLT ECQPNCN

1

CTTNTFSLSDAVGNHGGWCTLTKECQPNCN

30

86.7%

NZ_CP016795.1and 5 others:

NZ_CP016795.1 Lanthipeptides: BC342_RS18360_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18960_lanthipeptideNZ_CP036534.1 Lanthipeptides: EZV63_RS18960_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01495_lanthipeptideNZ_CP094676.1 Lanthipeptides: L6J92_RS01495_lanthipeptide

Lanthipeptides: BC342_RS18360_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD LGN GGWCTLT ECQPNC+

1

CTTNTFSLSDALGNHGGWCTLTKECQPNCS

30

86.7%

NZ_CP043317.1and 1 other:

NZ_CP043317.1 Lanthipeptides: FYC74_RS17680_lanthipeptide

Lanthipeptides: FYC74_RS17680_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLSD LGN GGWCTLT ECQPNC+

1

CTTNTFSLSDSLGNHGGWCTLTKECQPNCS

30

86.7%

NZ_CP047419.1and 3 others:

NZ_CP047419.1 Lanthipeptides: GTU71_RS12805_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptideNZ_PSTO01000045.1 Lanthipeptides: C5C10_RS15590_lanthipeptide

Lanthipeptides: GTU71_RS12805_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTF+LS+VLGN GGWCTLTVECQPNC

1

CTTNTFTLSNVLGNQGGWCTLTVECQPNCK

30

80.0%

NZ_LT985747.1and 1 other:

NZ_LT985747.1 Lanthipeptides: C8268_RS00165_lanthipeptide

Lanthipeptides: C8268_RS00165_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTF+LSD LGN GGWCTLT ECQ +CN

1

CTTNTFTLSDYLGNKGGWCTLTKECQRSCN

30

76.7%

NZ_CP084530.1and 1 other:

NZ_CP084530.1 Lanthipeptides: LDO05_RS02075_lanthipeptide

Lanthipeptides: LDO05_RS02075_lanthipeptide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTF+LS+ LGN GGWCTLT ECQ +CN

1

CTTNTFTLSNYLGNNGGWCTLTKECQRSCN

30

MIBiG 4.0 matches for H181DRAFT_RS07545

86.7%

BGC0002110.3 (QKV49893.1):

Lanthipeptide: birimositide

1

CTTNTFSLSDVLGNTGGWCTLTVECQPNCN

30

CTTNTFSLS+ LGN GGWCTLT ECQPNCN

1

CTTNTFSLSNALGNNGGWCTLTKECQPNCN

30

50.0%

BGC0000517.4 (BAB04173.1):

Lanthipeptide: haloduracin β or haloduracin α

6

FSLSDVLGNTGGWCTLTVECQPNCN

30

+++S LGN G +CTLTVEC P+CN

4

YNISCRLGNKGAYCTLTVECMPSCN

28

25.6%

BGC0001229.4 (SBI_06990):

Lanthipeptide: SBI-06990 A1 or SBI-06989 A2

19

CTLTVECQPNCN

30

CTL+ ECQPNCN

28

CTLSKECQPNCN

39

allorf_498973_499089

Leader:

MRACLRVTVAWSRIGGPVAGA

Core:

VCFTSATPCGGSVKRSR

Core with dehydrations:

VCysFDhbDhaADhbPCysGGDhaVKRDhaR

antiSMASH-DB 4.0 matches for allorf_498973_499089

100.0%

NZ_BMMP01000005.1and 3 others:

NZ_BMMP01000005.1 Lanthipeptides: allorf_174812_174928_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: allorf_498973_499089_lanthipeptideNZ_FMHJ01000003.1 Lanthipeptides: allorf_498973_499089_lanthipeptide

Lanthipeptides: allorf_174812_174928_lanthipeptide

1

VCFTSATPCGGSVKRSR

17

VCFTSATPCGGSVKRSR

1

VCFTSATPCGGSVKRSR

17

allorf_511535_511666

Leader:

MKAAERISESAA

Core:

CWRRTSSHGLHVRGTVSRDVPILTIPGDLYD

Core with dehydrations:

CysWRRDhbDhaDhaHGLHVRGDhbVDhaRDVPILDhbIPGDLYD

antiSMASH-DB 4.0 matches for allorf_511535_511666

100.0%

NZ_FMHJ01000003.1and 1 other:

NZ_FMHJ01000003.1 Lanthipeptides: allorf_511535_511666_lanthipeptide

Lanthipeptides: allorf_511535_511666_lanthipeptide

1

CWRRTSSHGLHVRGTVSRDVPILTIPGDLYD

31

CWRRTSSHGLHVRGTVSRDVPILTIPGDLYD

1

CWRRTSSHGLHVRGTVSRDVPILTIPGDLYD

31

78.8%

NZ_BMMP01000005.1and 1 other:

NZ_BMMP01000005.1 Lanthipeptides: allorf_162219_162356_lanthipeptide

Lanthipeptides: allorf_162219_162356_lanthipeptide

1

CWRRTSSHGLHVRGTVSRDVPILTIPGDLYD

31

CWRRTSSHGLHVRGT S+DVPILT+PGD++D

1

CWRRTSSHGLHVRGTDSQDVPILTVPGDMHD

31

25.8%

NZ_CP022203.1:

Lanthipeptides: allorf_3832712_3832861_lanthipeptide

16

VSRD-VPILTIPGDLYD

31

++RD VP++ +PG L D

3

LTRDTVPVVVVPGCLSD

19

with 1 more sequence matches

22.6%

NZ_CP071868.1:

Lassopeptides: J4E96_RS19650_lassopeptide

13

RGTVSRDVPILTIP

26

RG + R+ P LT+P

3

RGVLEREAPSLTLP

16

allorf_511619_511831

Leader:

MAPSWARRRAVRVICSAVRVRAPGA

Core:

DGARPPGERAGLCADGQGRTGSPTEPVVPRSVVQIAWNRQDRHVP

Core with dehydrations:

DGARPPGERAGLCysADGQGRDhbGDhaPDhbEPVVPRDhaVVQIAWNRQDRHVP

antiSMASH-DB 4.0 matches for allorf_511619_511831

100.0%

NZ_FMHJ01000003.1and 1 other:

NZ_FMHJ01000003.1 Lanthipeptides: allorf_511619_511831_lanthipeptide

Lanthipeptides: allorf_511619_511831_lanthipeptide

1

DGARPPGERAGLCADGQGRTGSPTEPVVPRSVVQIAWNRQDRHVP

45

DGARPPGERAGLCADGQGRTGSPTEPVVPRSVVQIAWNRQDRHVP

1

DGARPPGERAGLCADGQGRTGSPTEPVVPRSVVQIAWNRQDRHVP

45

23.7%

NZ_CP031058.1:

Lanthipeptides: allorf_3313863_3314099_lanthipeptide

6

PGERAGLCADGQGRTGSPTEPVVP------RSVVQIAWNRQDRHV

44

P RA CA G+ R G +P R I W RH+

4

PCSRALSCALGKTRVGLLAQPCRAHAPNPWRQRSWIGWPAASRHI

48

22.2%

NC_012489.1:

Lanthipeptides: allorf_4594448_4594576_lanthipeptide

2

GARPPGERAGLCADGQGRTGSPTEP

26

G RPP G C T PT P

8

GCRPPSPEHGGCPTMPSDTALPTRP

32

with 4 more sequence matches

20.0%

NZ_CP110813.1:

Lanthipeptides: allorf_5686197_5686325_lanthipeptide

18

GRTGSPTEPVVPRSVVQIAWNRQDRHV

44

GRT P PRS+V+++ + R +

1

GRTFVPKPETRPRSIVRLSTHPMTRRI

27

15.6%

NC_007622.1and 9 others:

NC_017337.1 Lanthipeptides: SAOV_RS09490_lanthipeptideNZ_CP038268.1 Lanthipeptides: SaO55_RS09725_lanthipeptideNZ_CP047792.1 Lanthipeptides: E3S88_RS07350_lanthipeptideNZ_CP094743.1 Lanthipeptides: MUA62_RS09295_lanthipeptideNZ_CP102962.1 Lanthipeptides: NW971_RS09560_lanthipeptideNZ_GG730342.1 Lanthipeptides: SAUG_RS17380_lanthipeptideNZ_JAGSAY010000004.1 Lanthipeptides: KC186_RS06750_lanthipeptideNZ_LR133917.1 Lanthipeptides: ELZ45_RS09165_lanthipeptideNZ_LS483317.1 Lanthipeptides: DQL75_RS09705_lanthipeptide

Lanthipeptides: SAB_RS08995_lanthipeptide

12

LCADGQGRTGS

22

LC+ G G+TGS

7

LCSFGCGKTGS

17

15.6%

NZ_KV802313.1and 1 other:

NZ_KV817389.1 Lanthipeptides: allorf_50141_50287_lanthipeptide

Lanthipeptides: allorf_165895_166041_lanthipeptide

17

QGRTGSPTEPVVPRSVV

33

QG +GS T P R +

7

QGSSGSLTSPASERYCI

23

15.6%

NZ_LR134304.1:

Lanthipeptides: EL116_RS09250_lanthipeptide

12

LCADGQGRTGS

22

LC+ G G TGS

7

LCSYGCGNTGS

17

MIBiG 4.0 matches for allorf_511619_511831

11.1%

BGC0000582.4 (ADR72962.1):

Linaridin: cypemycin

21

GSPTEPVVPRSVVQ

34

+P P V + V+Q

1

ATPATPTVAQFVIQ

14

allorf_516193_516348

Leader:

MAESSSSDTGRECAEGPAEAGS

Core:

GEERSRGRGKRLRNALCGGWPGRRAPQGS

Core with dehydrations:

GEERDhaRGRGKRLRNALCysGGWPGRRAPQGDha

antiSMASH-DB 4.0 matches for allorf_516193_516348

100.0%

NZ_FMHJ01000003.1:

Lanthipeptides: allorf_516193_516348_lanthipeptide

1

GEERSRGRGKRLRNALCGGWPGRRAPQGS

29

GEERSRGRGKRLRNALCGGWPGRRAPQGS

1

GEERSRGRGKRLRNALCGGWPGRRAPQGS

29

26.5%

NZ_CP031447.1:

Lanthipeptides: allorf_0154812_0155045_lanthipeptide

4

RSRGRGK-----RLRNALCGGWPGRRAP

26

RSR RG+ R+ + G PGRRAP

19

RSRLRGEACALVRIVGCVDDGGPGRRAP

46

19.6%

NC_009621.1and 4 others:

NZ_CP088111.1 Lanthipeptides: allorf_0786726_0786959_lanthipeptideNZ_NBUK01000063.1 Lanthipeptides: allorf_6661_6894_lanthipeptideNZ_VISX01000063.1 Lanthipeptides: allorf_1862_2095_lanthipeptideNZ_VITA01000039.1 Lanthipeptides: allorf_06616_06849_lanthipeptide

Lanthipeptides: allorf_1020482_1020715_lanthipeptide

5

SRGRGKRLRNALCGGWPGRRAPQG

28

++ KRL +C P RR P G

23

TKSLIKRLMMLVCP--PSRRVPPG

44

with 1 more sequence matches

17.2%

NZ_MSKU01000037.1:

Lanthipeptides: allorf_43371_43508_lanthipeptide

10

KRLRNALCGGWPG

22

+ N C GWP

2

STISNEHCWGWPS

14

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.96 out of 20.97

Confidence: medium

Additional weak hits (10):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.12 out of 29.38

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.93 out of 27.55

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.01 out of 20.97

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.96 out of 21.55

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.44 out of 24.78

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.88 out of 28.14

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.21 out of 24.78

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.14 out of 32.93

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.07 out of 26.85

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.17 out of 28.19

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

H181DRAFT_RS29345 - Class II

Cleavage pHMM score: -2.10

RODEO score: 0

Monoisotopic mass: 4178.9 Da

Molecular weight: 4181.6 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 4199.6 Da N = 1; 4217.7 Da N = 2; 4235.7 Da N = 3; 4253.7 Da N = 4; 4271.7 Da N = 5

H181DRAFT_RS07545 - Class II

Cleavage pHMM score: -3.70

RODEO score: 0

Monoisotopic mass: 3033.4 Da

Molecular weight: 3035.3 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3053.4 Da N = 1; 3071.4 Da N = 2; 3089.4 Da N = 3; 3107.4 Da N = 4

allorf_498973_499089 - Class II

Cleavage pHMM score: -3.40

RODEO score: 0

Monoisotopic mass: 1664.9 Da

Molecular weight: 1665.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1683.9 Da N = 1; 1702.0 Da N = 2; 1720.0 Da N = 3

allorf_511535_511666 - Class II

Cleavage pHMM score: -6.90

RODEO score: 0

Monoisotopic mass: 3397.8 Da

Molecular weight: 3399.8 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3417.8 Da N = 1; 3435.9 Da N = 2; 3453.9 Da N = 3; 3471.9 Da N = 4; 3489.9 Da N = 5

allorf_511619_511831 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 4716.4 Da

Molecular weight: 4719.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4737.2 Da N = 1; 4755.2 Da N = 2; 4773.2 Da N = 3

allorf_516193_516348 - Class II

Cleavage pHMM score: -4.40

RODEO score: 0

Monoisotopic mass: 3127.7 Da

Molecular weight: 3129.5 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3147.5 Da N = 1

H181DRAFT_RS29345 - Class II

Cleavage pHMM score: -2.10

RODEO score: 0

Monoisotopic mass: 4178.9 Da

Molecular weight: 4181.6 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 4199.6 Da N = 1; 4217.7 Da N = 2; 4235.7 Da N = 3; 4253.7 Da N = 4; 4271.7 Da N = 5

H181DRAFT_RS07545 - Class II

Cleavage pHMM score: -3.70

RODEO score: 0

Monoisotopic mass: 3033.4 Da

Molecular weight: 3035.3 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3053.4 Da N = 1; 3071.4 Da N = 2; 3089.4 Da N = 3; 3107.4 Da N = 4

allorf_493830_493922 - Class II

Cleavage pHMM score: -3.10

RODEO score: 0

Monoisotopic mass: 1079.6 Da

Molecular weight: 1080.2 Da

Number of bridges: 1

allorf_498973_499089 - Class II

Cleavage pHMM score: -3.40

RODEO score: 0

Monoisotopic mass: 1664.9 Da

Molecular weight: 1665.9 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 1683.9 Da N = 1; 1702.0 Da N = 2; 1720.0 Da N = 3

allorf_511535_511666 - Class II

Cleavage pHMM score: -6.90

RODEO score: 0

Monoisotopic mass: 3397.8 Da

Molecular weight: 3399.8 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 3417.8 Da N = 1; 3435.9 Da N = 2; 3453.9 Da N = 3; 3471.9 Da N = 4; 3489.9 Da N = 5

allorf_511619_511831 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 4716.4 Da

Molecular weight: 4719.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 4737.2 Da N = 1; 4755.2 Da N = 2; 4773.2 Da N = 3

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A

NZ_FMHJ01000009.1 - Region 1 - NRPS,NRPS-like

Download region SVG

Location: 1 - 39,289 nt. (total: 39,289 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

H181DRAFT_RS17515: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

H181DRAFT_RS17635: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

H181DRAFT_RS17640: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Transferases: aminotransferase halogenase

H181DRAFT_RS17520: aminotransferase

smcogs: SMCOG1019: aminotransferase

H181DRAFT_RS17525: conventional flavin-dependent halogenase

halogenases: conventional flavin-dependent halogenase
MITE: MITE0000030Halogenation (identity: 62.30%)
smcogs: SMCOG1119: halogenase

Hydrolases: deacetylase esterase hydrolase

H181DRAFT_RS17510: thioesterase

smcogs: SMCOG1004: thioesterase

H181DRAFT_RS17575: thioesterase

smcogs: SMCOG1004: thioesterase

H181DRAFT_RS17595: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

H181DRAFT_RS17605: acetylornithine deacetylase

smcogs: SMCOG1247: acetylornithine deacetylase

Ligases: ligase

H181DRAFT_RS17505: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS17545: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS17550: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS17555: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.83	NRPS	dimethylcoprogen	Alternaria alternata
BGC0000412.5	0.77	NRPS	pyochelin	Pseudomonas aeruginosa PAO1
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.68	NRPS	AM-toxin	Alternaria alternata
BGC0001641.4	0.67	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.65	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0000412.5	0.71	NRPS	pyochelin	Pseudomonas aeruginosa PAO1
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.68	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.67	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.67	NRPS	AM-toxin	Alternaria alternata
BGC0001641.4	0.66	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.66	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001833.4	0.66	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.70 out of 26.85

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.88 out of 23.79

Confidence: medium

Additional weak hits (11):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.16 out of 30.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.39 out of 29.25

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 19.60 out of 26.12

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.38 out of 36.54

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 17.73 out of 30.25

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.90 out of 30.22

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.46 out of 20.97

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.44 out of 36.54

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.71 out of 29.65

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.86 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 39289:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Trp) + (Cys) + (Cys)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 30524:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Trp) + (Cys) + (Cys)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

H181DRAFT_RS17505: X

AMP-binding (32..433): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NACNAALGCPGGTLQVGSSMNVYGIGEGLITHTR
Nearest Stachelhaus code(s):: Hqa ALPTQGWLTK (76% 8Å match); COOH-Qui ALPTQGWLTK (74% 8Å match)
Stachelhaus code match:: 80% (moderate)

H181DRAFT_RS17545: Trp

Search NORINE for peptide: strict or relaxed

AMP-binding (7..400): Trp

nrpys: Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LTQAFDAWFWDLVLTSTGDFNAYGPTEVTVGASI
Nearest Stachelhaus code(s):: Trp DAWVMTGVGK (85% 8Å match); Kyn DAWTTTGVGK (71% 8Å match)
Stachelhaus code match:: 80% (moderate)

H181DRAFT_RS17550: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (578..975): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDISFDLSVWDGNTLLSGDVALGGATEAAIWSNA
Nearest Stachelhaus code(s):: Cys DLWNLSLIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS17555: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (546..940): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDIAFDLSVYDGNSLLSGDVSLGGATEAAIWSIH
Nearest Stachelhaus code(s):: Cys DLYNLSLIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism
RutR	Regulator of the pyrimidine and purine metabolism

NZ_FMHJ01000023.1 - Region 1 - NI-siderophore

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Location: 1 - 17,817 nt. (total: 17,817 nt) Show pHMM detection rules used

Region on contig edge.

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

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Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

H181DRAFT_RS27885: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

Lyases: decarboxylase

H181DRAFT_RS27890: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001478.5	0.84	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0002305.2	0.80	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000941.3	0.79	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000940.5	0.72	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001453.5	0.71	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0001572.3	0.70	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002464.3	0.61	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0002073.3	0.60	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0002690.2	0.58	other (other)	FW0622	Verrucosispora sp. FIM060022
BGC0002620.2	0.55	other (other)	fulvivirgamide A2, fulvivirgamide B2, fulvivirgamide B3, fulvivirgamide B4	Fulvivirga marina

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001478.5	0.84	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0002305.2	0.80	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000941.3	0.79	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000940.5	0.72	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001453.5	0.71	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0001572.3	0.70	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002464.3	0.61	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0002073.3	0.60	NRPS	dehydroxynocardamine	Corynebacterium propinquum
BGC0002690.2	0.58	other (other)	FW0622	Verrucosispora sp. FIM060022
BGC0002620.2	0.55	other (other)	fulvivirgamide A2, fulvivirgamide B2, fulvivirgamide B3, fulvivirgamide B4	Fulvivirga marina

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 29.62 out of 36.53

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 28.73 out of 36.53

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (7):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.33 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.77 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.58 out of 26.06

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.39 out of 35.63

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.45 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.14 out of 36.53

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
DmdR1	Iron(II)-dependent repressor

NZ_FMHJ01000006.1 - Region 1 - NRPS,butyrolactone,transAT-PKS

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Location: 1 - 81,431 nt. (total: 81,431 nt) Show pHMM detection rules used

Region on contig edge.

transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
butyrolactone: AfsA

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

H181DRAFT_RS12330: Heterocyclization

MITE: MITE0000043Heterocyclization (identity: 88.90%)
smcogs: SMCOG1007: cytochrome P450

H181DRAFT_RS12385: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

H181DRAFT_RS28805: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: PPTase acylase

H181DRAFT_RS12360: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

H181DRAFT_RS12390: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: esterase

H181DRAFT_RS12365: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

H181DRAFT_RS12290: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS12295: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

H181DRAFT_RS12305: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

H181DRAFT_RS12340: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

H181DRAFT_RS12345: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

H181DRAFT_RS12350: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

H181DRAFT_RS12355: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

H181DRAFT_RS12370: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000848.5	1.47	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000849.4	1.22	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0001535.3	1.00	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0001534.3	0.87	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000342.4	0.83	NRPS	enniatin	Fusarium equiseti
BGC0000850.5	0.82	other (other)	γ-butyrolactone	Streptomyces acidiscabies
BGC0000465.4	0.72	NRPS	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000426.5	0.69	NRPS	sevadicin	Paenibacillus larvae
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.69	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001535.3	0.78	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000848.5	0.78	other (other)	A-factor	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001534.3	0.74	other (other)	branched-chain fatty acids	Streptomyces filamentosus
BGC0000342.4	0.73	NRPS	enniatin	Fusarium equiseti
BGC0000849.4	0.70	other (butyrolactone)	SCB1, SCB2, SCB3	Streptomyces coelicolor A3(2)
BGC0000465.4	0.69	NRPS	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0000426.5	0.68	NRPS	sevadicin	Paenibacillus larvae
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (18):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.46 out of 30.22

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.25 out of 30.25

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 13.79 out of 27.78

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.31 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.08 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 12.89 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 14.55 out of 33.14

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.86 out of 19.52

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 13.84 out of 27.78

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 16.81 out of 36.54

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.88 out of 21.84

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.98 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 15.34 out of 29.38

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.79 out of 30.22

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.21 out of 28.19

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 19.41 out of 29.65

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 81431:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (NMe-Gly) + (D-X) + (ccmal - ccmal - Gly) + (mal) + (ohmal - mal) + (Ser - mal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

H181DRAFT_RS12290: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (465..857): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: FDMTFDIAALELQALTGGELNLYGPTETTIWSTA
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS12295: X

AMP-binding (469..879): X

nrpys: 4OH-Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWYSFDPAPLECYTVQAGEHNLYGPTETHVVTAG
Nearest Stachelhaus code(s):: 4OH-Pro DPLYVALVVK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS28805: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (3976..4389): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: TAFTFDISVLELQALCGGEWNVYGPTEATIWSTA
Nearest Stachelhaus code(s):: Gly DILQLGVIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS12350: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (975..1376): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWATFDVSVWEWHFLASGEHNLYGPTEAAVDVTH
Nearest Stachelhaus code(s):: Ser DVWHLSLVDK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS12360: mal

PKS_AT (5..288): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 83.3%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 98.0
Methoxymalonyl-CoA: 27.2
inactive: 19.8
Methylmalonyl-CoA: 18.1
trans-1,2-CPDA: 9.9
Propionyl-CoA: 8.7
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

H181DRAFT_RS12370: Cys

Search NORINE for peptide: strict or relaxed

AMP-binding (293..697): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TDISFDLSVYDGNTLLSGDVSAGGATEAAIWSIC
Nearest Stachelhaus code(s):: Cys DLYNLSAIWK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description

NZ_FMHJ01000006.1 - Region 2 - terpene

Download region SVG

Location: 263,936 - 290,559 nt. (total: 26,624 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

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Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

H181DRAFT_RS13335: dehydrogenase

smcogs: SMCOG1181: dehydrogenase

Transferases: aminotransferase transferase

H181DRAFT_RS13265: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

H181DRAFT_RS13340: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Ligases: synthetase

H181DRAFT_RS13285: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Translocases: transport

H181DRAFT_RS13310: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000663.5	0.65	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000664.5	0.50	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000656.5	0.50	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000646.5	0.49	terpene	β-carotein	uncultured bacterium
BGC0000640.3	0.48	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0002173.2	0.48	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0000859.3	0.48	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000856.3	0.47	other (other)	ectoine	Methylophaga thalassica
BGC0000855.3	0.47	other (other)	ectoine	Methylomicrobium kenyense

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000663.5	0.65	terpene	hopene	Streptomyces coelicolor A3(2)
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000664.5	0.50	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000656.5	0.50	terpene	zeaxanthin	Xanthobacter autotrophicus Py2
BGC0000646.5	0.49	terpene	β-carotein	uncultured bacterium
BGC0000640.3	0.48	terpene	carotenoid	Enterobacteriaceae bacterium DC404
BGC0002173.2	0.48	saccharide, terpene	fumihopaside A, compound 3, 21-β-H-hopane-3beta, 22-diol	Aspergillus fumigatus A1163
BGC0000859.3	0.48	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000856.3	0.47	other (other)	ectoine	Methylophaga thalassica
BGC0000855.3	0.47	other (other)	ectoine	Methylomicrobium kenyense

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
triterpene	hopanoid		C30	hopanoid skeleton	unknown
triterpene	polypodane		C30	polypodane skeleton	unknown

Genes with terpene domains:

H181DRAFT_RS13280: T2TS

Domain 1 (49 - 684): Type 2 terpene synthase

Subtype: Squalene substrate

Reactions:: squalene → hopanoid; squalene → polypodane

H181DRAFT_RS13285: PT_FPPS_like

Domain 1 (80 - 374): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

H181DRAFT_RS13295: PT_phytoene_like

Domain 1 (9 - 303): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → PSPP; FPP → squalene; FPP → diapophytoene; GGPP → phytoene; PSPP → hydroxysqualene

H181DRAFT_RS13300: PT_phytoene_like

Domain 1 (8 - 283): Prenyltransferase; Phytoene synthase like

Subtype: Hydroxysqualene synthase

Reactions:: PSPP → hydroxysqualene

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.23 out of 19.52

Confidence: medium

Additional weak hits (1):

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.56 out of 23.43

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production

NZ_FMHJ01000008.1 - Region 1 - NRP-metallophore,NRPS

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Location: 254,646 - 303,480 nt. (total: 48,835 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Select a gene to view the details available for it

Gene overview

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MIBiG comparison

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SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

H181DRAFT_RS17100: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

H181DRAFT_RS17120: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Transferases: glycosyltransferase transferase

H181DRAFT_RS17035: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

H181DRAFT_RS17045: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Hydrolases: esterase hydrolase

H181DRAFT_RS17030: enterobactin/ferric enterobactin esterase

smcogs: SMCOG1098: enterobactin/ferric enterobactin esterase

H181DRAFT_RS17085: isochorismatase

smcogs: SMCOG1027: isochorismatase

Lyases: dehydratase

H181DRAFT_RS17050: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

H181DRAFT_RS17055: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Isomerases: isomerase

H181DRAFT_RS17095: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase

H181DRAFT_RS17075: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

H181DRAFT_RS17090: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001185.5	0.78	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0000343.5	0.71	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.69	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000401.5	0.67	NRPS	paenibactin	Paenibacillus elgii B69
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001641.4	0.64	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001185.5	0.71	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0000343.5	0.68	NRPS	enterobactin	Pseudomonas sp. J465
BGC0001833.4	0.67	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.67	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.67	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0000401.5	0.67	NRPS	paenibactin	Paenibacillus elgii B69
BGC0001128.4	0.67	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.67	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001641.4	0.65	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.22 out of 18.46

Confidence: strong

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 32.19 out of 36.53

Confidence: strong

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 33.07 out of 36.53

Confidence: strong

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 31.36 out of 36.53

Confidence: medium

Additional weak hits (10):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 16.79 out of 30.97

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.66 out of 30.22

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.04 out of 29.65

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.68 out of 39.62

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.41 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.84 out of 36.53

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.41 out of 25.75

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.62 out of 33.14

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.64 out of 35.63

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.25 out of 20.01

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 254645 - 303480:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

H181DRAFT_RS17075: X

AMP-binding (503..905): X

nrpys: Ala, Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFCSGEFNLYGPTEAAVDVTH
Nearest Stachelhaus code(s):: Ala or Ser DVWHFSLVDK (97% 8Å match); Ser DVWHFSLVDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

H181DRAFT_RS17090: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (32..444): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SASLFPLSSPGGGLLVGGAQQVFGMAEGLVNYTR
Nearest Stachelhaus code(s):: diOH-Bz PLPGLGVVNK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
DmdR1	Iron(II)-dependent repressor
RpoN	Regulator of nitrogen assimilation and virulence

NZ_FMHJ01000008.1 - Region 2 - RiPP-like

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Location: 314,001 - 324,213 nt. (total: 10,213 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001555.4	0.47	ribosomal	colicin V	Escherichia coli chi7122
BGC0000590.5	0.46	ribosomal	microcin N	Escherichia coli
BGC0000554.4	0.45	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000588.5	0.45	ribosomal	microcin L	Escherichia coli
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002698.2	0.41	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000484.5	0.39	ribosomal	glycocin F	Lactobacillus plantarum
BGC0000617.5	0.38	ribosomal	coagulin	Bacillus coagulans
BGC0000589.5	0.37	ribosomal	microcin M	Escherichia coli Nissle 1917

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001555.4	0.47	ribosomal	colicin V	Escherichia coli chi7122
BGC0000590.5	0.46	ribosomal	microcin N	Escherichia coli
BGC0000554.4	0.45	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000588.5	0.45	ribosomal	microcin L	Escherichia coli
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0002698.2	0.41	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0000484.5	0.39	ribosomal	glycocin F	Lactobacillus plantarum
BGC0000617.5	0.38	ribosomal	coagulin	Bacillus coagulans
BGC0000589.5	0.37	ribosomal	microcin M	Escherichia coli Nissle 1917

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.86 out of 23.79

Confidence: medium

Additional weak hits (5):

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.95 out of 30.25

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.66 out of 20.97

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.22 out of 35.63

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.86 out of 19.52

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NZ_FMHJ01000008.1 - Region 3 - terpene

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Location: 334,674 - 355,852 nt. (total: 21,179 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

H181DRAFT_RS17395: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

H181DRAFT_RS17400: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: methyltransferase

H181DRAFT_RS17355: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: cyclase

H181DRAFT_RS17360: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000657.5	0.73	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000659.3	0.63	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0000658.3	0.61	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002631.2	0.53	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0001310.5	0.51	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0001910.4	0.51	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001911.3	0.48	terpene	raimonol	Streptomyces anulatus
BGC0000844.5	0.45	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0001986.3	0.44	other (other)	purincyclamide	Streptomyces chrestomyceticus
BGC0001197.5	0.43	PKS	frankiamicin	Frankia sp. EAN1pec

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000657.5	0.73	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000659.3	0.63	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0000658.3	0.61	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002631.2	0.53	ribosomal	Myxarylin	Pyxidicoccus fallax
BGC0001310.5	0.51	PKS	naringenin	Streptomyces clavuligerus ATCC 27064
BGC0001910.4	0.51	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001911.3	0.48	terpene	raimonol	Streptomyces anulatus
BGC0000844.5	0.45	other (other)	clavulanic acid	Streptomyces clavuligerus
BGC0001986.3	0.44	other (other)	purincyclamide	Streptomyces chrestomyceticus
BGC0001197.5	0.43	PKS	frankiamicin	Frankia sp. EAN1pec

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
monoterpene		2-methylisoborneol	C11	C1-C6	OH
monoterpene		2-methylenebornane	C11	C1-C6	none

Genes with terpene domains:

H181DRAFT_RS17360: T1TS

Domain 1 (64 - 388): Type 1 terpene synthase

Subtype: 2-Methylisoborneol synthase type

Reactions:: 2-MeGPP → 2-methylisoborneol; 2-MeGPP → 2-methylenebornane

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.13 out of 19.26

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

Additional weak hits (1):

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.52 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
2-MeGPP	2-methylgeranyl diphosphate

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
VqsM	Regulator of quorum-sensing signalling systems

NZ_FMHJ01000012.1 - Region 1 - NRPS,T1PKS,hglE-KS

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Location: 1 - 86,990 nt. (total: 86,990 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
hglE-KS: (hglE or hglD)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

H181DRAFT_RS20945: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

H181DRAFT_RS21080: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

H181DRAFT_RS21175: aldehyde oxidase and xanthine dehydrogenase

smcogs: SMCOG1293: aldehyde oxidase and xanthine dehydrogenase

Transferases: acylase aminotransferase carbamoyltransferase glycosyltransferase

H181DRAFT_RS20930: ornithine carbamoyltransferase

smcogs: SMCOG1114: ornithine carbamoyltransferase

H181DRAFT_RS20985: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

H181DRAFT_RS21000: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

H181DRAFT_RS21110: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: dehalogenase hydrolase

H181DRAFT_RS20990: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

H181DRAFT_RS21155: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

H181DRAFT_RS21160: isochorismatase

smcogs: SMCOG1027: isochorismatase

Lyases: hydratase

H181DRAFT_RS20920: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: synthase

H181DRAFT_RS21005: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

H181DRAFT_RS21115: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001244.3	1.16	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001160.5	1.08	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	1.07	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001254.3	0.99	PKS	ACT-Toxin II	Alternaria alternata
BGC0000041.3	0.77	PKS	oronofacic acid	Pseudomonas syringae
BGC0000869.5	0.71	other (other)	heterocyst glycolipids	Nostoc sp. 'Peltigera membranacea cyanobiont'
BGC0001161.5	0.65	PKS	1-heptadecene	Moorea bouillonii PNG
BGC0001162.3	0.65	PKS	1-heptadecene	Moorea producens JHB
BGC0001163.3	0.65	PKS	1-heptadecene	Moorea producens 3L
BGC0000865.3	0.64	other (other)	eicoseicosapentaenoic acid	Photobacterium profundum SS9

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0002135.2	0.63	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.63	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae
BGC0001275.3	0.62	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000357.5	0.62	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.03 out of 29.72

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.52 out of 19.26

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 19.02 out of 23.79

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.75 out of 23.05

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (22):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.13 out of 28.14

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.19 out of 21.55

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.87 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.27 out of 27.78

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.57 out of 26.33

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.53 out of 32.93

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 22.52 out of 36.53

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 19.14 out of 22.40

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.91 out of 23.43

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.03 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.29 out of 28.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.21 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.74 out of 33.14

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.42 out of 23.43

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.68 out of 24.78

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.00 out of 35.63

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 16.62 out of 29.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.93 out of 29.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.78 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.90 out of 28.19

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.55 out of 36.53

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 0 - 86990:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Val) + (mal) + (pk)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 42550:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (pk)
Non-colinear ordering:: H181DRAFT_RS21005 + H181DRAFT_RS21000 + H181DRAFT_RS20995

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 2594 - 49800:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (mal) + (pk)
Non-colinear ordering:: H181DRAFT_RS21005 + H181DRAFT_RS21000 + H181DRAFT_RS20995

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 36071 - 86990:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (Val)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

H181DRAFT_RS21000: pk

PKS_AT (595..828): pk

ATSignature: (unknown)

No matches above 50%

minowa: inactive

Prediction, score:

inactive: 14.7
3-Methylbutyryl-CoA: 11.5
Malonyl-CoA: 8.6
Isobutyryl-CoA: 7.0
Methoxymalonyl-CoA: 5.5
fatty_acid: 5.3
Methylmalonyl-CoA: 1.5
trans-1,2-CPDA: 0.0
Propionyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
CHC-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

H181DRAFT_RS21005: mal

PKS_AT (1234..1527): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 89.5
inactive: 41.2
Methoxymalonyl-CoA: 39.2
Methylmalonyl-CoA: 32.1
Propionyl-CoA: 20.1
CHC-CoA: 13.3
fatty_acid: 10.2
Ethylmalonyl-CoA: 8.7
2-Methylbutyryl-CoA: 5.6
trans-1,2-CPDA: 0.0
Isobutyryl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

H181DRAFT_RS21110: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (1415..1813): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWHCFDASLLEPMLVCGGEVNVYGPSEVTVLATT
Nearest Stachelhaus code(s):: Val DALFVGIVLK (68% 8Å match); aIle DALVFGVVLK (56% 8Å match)
Stachelhaus code match:: 80% (moderate)

H181DRAFT_RS21115: mal

PKS_AT (599..881): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 58.3%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 127.1
Methylmalonyl-CoA: 71.0
Methoxymalonyl-CoA: 68.3
inactive: 60.7
Propionyl-CoA: 42.0
Ethylmalonyl-CoA: 35.0
CHC-CoA: 33.5
trans-1,2-CPDA: 32.7
Isobutyryl-CoA: 29.7
2-Methylbutyryl-CoA: 26.0
Acetyl-CoA: 20.8
fatty_acid: 19.2
Benzoyl-CoA: 14.2
3-Methylbutyryl-CoA: 12.4

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism
NtrC	Nitrogen regulatory protein C

NZ_FMHJ01000012.1 - Region 2 - NI-siderophore

Download region SVG

Location: 173,067 - 205,810 nt. (total: 32,744 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

H181DRAFT_RS21535: Antibiotic biosynthesis monooxygenase

smcogs: SMCOG1258: Antibiotic biosynthesis monooxygenase

Transferases: methyltransferase

H181DRAFT_RS21565: methyltransferase

smcogs: SMCOG1248: methyltransferase

Isomerases: mutase

H181DRAFT_RS21530: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.55	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0000942.4	0.54	other (other)	petrobactin	Bacillus anthracis str. Ames
BGC0001713.5	0.52	other (other)	ochrobactin	Ochrobactrum anthropi
BGC0000295.5	0.51	NRPS	acinetoferrin	Acinetobacter haemolyticus
BGC0001531.5	0.51	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0001572.3	0.50	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002682.2	0.50	other (other)	aerobactin	Vibrio mimicus
BGC0002305.2	0.49	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0002633.2	0.47	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0002683.2	0.47	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.55	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0000942.4	0.54	other (other)	petrobactin	Bacillus anthracis str. Ames
BGC0001713.5	0.52	other (other)	ochrobactin	Ochrobactrum anthropi
BGC0000295.5	0.51	NRPS	acinetoferrin	Acinetobacter haemolyticus
BGC0001531.5	0.51	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0001572.3	0.50	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002682.2	0.50	other (other)	aerobactin	Vibrio mimicus
BGC0002305.2	0.49	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0002633.2	0.47	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0002683.2	0.47	NRPS	schizokinen	Nostoc sp. PCC 7120 = FACHB-418

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.46 out of 20.47

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 22.14 out of 27.18

Confidence: medium

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 23.31 out of 27.18

Confidence: medium

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 20.93 out of 24.78

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.73 out of 18.46

Confidence: medium

Additional weak hits (8):

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.59 out of 26.85

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.38 out of 28.19

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.32 out of 27.18

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 23.09 out of 29.65

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.77 out of 25.88

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.66 out of 23.43

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.45 out of 32.93

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.88 out of 23.43

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
LexA variant 2	Repressor of DNA damage response
NrdR	Represses ribonucleotide reductase encoding genes
RpoN	Regulator of nitrogen assimilation and virulence
VqsM	Regulator of quorum-sensing signalling systems

NZ_FMHJ01000015.1 - Region 1 - terpene