LOCUS       NZ_FMHJ01000029         2995 bp    DNA     linear   CON 28-JUL-2024
DEFINITION  Streptomyces sp. WMMB 714, whole genome shotgun sequence.
ACCESSION   NZ_FMHJ01000029
VERSION     NZ_FMHJ01000029.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. WMMB 714
  ORGANISM  Streptomyces sp. WMMB 714
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,Neha. and Submissions,Spin.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2016) DOE - JOINT GENOME INSTITUTE, 2800 Mitchell
            Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900090125.1-RS_2024_07_28
            Annotation Date                   :: 07/28/2024 02:17:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,656
            CDSs (total)                      :: 5,578
            Genes (coding)                    :: 5,496
            CDSs (with protein)               :: 5,496
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 5, 4 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 15 of 82
            Pseudo Genes (incomplete)         :: 72 of 82
            Pseudo Genes (internal stop)      :: 4 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2995
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FMHJ01000029.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..2956)
                     /locus_tag="H181DRAFT_RS28170"
                     /old_locus_tag="H181DRAFT_05658"
     CDS             complement(<1..2956)
                     /NRPS_PKS="Domain: Condensation_LCL (20-322). E-value:
                     1e-119. Score: 390.9. Matches aSDomain:
                     nrpspksdomains_H181DRAFT_RS28170_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (491-889). E-value: 2.1e-99.
                     Score: 324.9. Matches aSDomain:
                     nrpspksdomains_H181DRAFT_RS28170_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="H181DRAFT_RS28170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_05658"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_158715471.1"
                     /sec_met_domain="Condensation (E-value: 4.2e-72, bitscore:
                     228.8, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.9e-108, bitscore:
                     348.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALEGAAAARTPLRPAERPAQIPLSPAQWRLWFLNQMEGPSPTY
                     NIPYALRLSGRLDEDALRAALDDVVARHESLRTLFPDEGGDPRQHIVPADRARVTVTAV
                     HTTEDGLAAALTAAGREGFDLAAELPLRVHLLRTGATECVLLLNLHHIAGDGWSMAPLA
                     RDLMTAYEARCAGGAPEWAPLPVQYADYALWQRELLGHESDPGSELSRQLGHWRERLAE
                     LPAEIALPTDRPRPPVAGYDGALVRFRVSEELHAGLASLARESSATLFMVVQAGLAALL
                     SRLGAGPDVAVGSPVAGRTDEALDDLVGFFVNTLVLRTDVSGDPSFRELLGRVRESDLA
                     AYAHQDVPFERLVEVLNPQRSLSRNPLVQVMLALQNMPRTETALPGLRVRPEPLDVGIS
                     KFDLSFHLRESLGADGTEGGIDGVLEYSTDLFDHATVRALADRLVAVLRQAVADPDRPV
                     GAVDLLSPAEYSRLEQWNDTAAGPVDATLPELLEAQTARTPDAVAVRHAGTDTRYAELN
                     ARANQLARHLVDAGVGGEDLVGIAMPRTPELLVAVWAVLKAGAAYVPLDLDYPAQRLTL
                     MLDDAAPAYVLTTRDGAARLPDAARRITLDDPGVREAVAAYPDGDLGDGERVRPLTPDN
                     AAYAIFTSGSTGRPKAVVVPQRNVADLAAWAEGEFGADGLAAVTATTSLSFDVSVFELF
                     SPLLCGGRVDLEDDVLALAGGDASRVSGMLSTVPSAMAALLADPELPLRPHTLLYAGEA
                     LTAGLVRDTRAALPGCRVLNVYGPTEATVYATMAEPSPDDPAQPAIGNPLRNVTAHILD
                     ANLRPLPPGSPGELYLAGELHLARGYLNRPGLTAERFVASPFGPPGSRMYRTGDLARQR
                     ADGSVEYLGRSDDQVKIRGFRIEPREIETALTALPTVRECLVTSLRDHEGEQRLAAYVV
                     PASSGGVTADELRDVAVDALPGHLVPSAFVVLERLPLTVNGKLDRAALPVPEFSG"
     source          1..2995
                     /db_xref="taxon:1286822"
                     /mol_type="genomic DNA"
                     /note="contig: H181DRAFT_scaffold00029.29"
                     /organism="Streptomyces sp. WMMB 714"
                     /strain="WMMB 714"
     region          1..2995
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2995
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2995
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:2956](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..2956)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(260..325)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0011"
                     /evalue="4.10E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="899"
                     /protein_start="877"
                     /score="38.0"
                     /tool="antismash"
                     /translation="YLGRSDDQVKIRGFRIEPREIE"
     aSDomain        complement(290..1483)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H181DRAFT_RS28170_AMP-binding.1"
                     /evalue="2.10E-99"
                     /label="H181DRAFT_RS28170_AMP-binding.1"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="889"
                     /protein_start="491"
                     /score="324.9"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="EAQTARTPDAVAVRHAGTDTRYAELNARANQLARHLVDAGVGGED
                     LVGIAMPRTPELLVAVWAVLKAGAAYVPLDLDYPAQRLTLMLDDAAPAYVLTTRDGAAR
                     LPDAARRITLDDPGVREAVAAYPDGDLGDGERVRPLTPDNAAYAIFTSGSTGRPKAVVV
                     PQRNVADLAAWAEGEFGADGLAAVTATTSLSFDVSVFELFSPLLCGGRVDLEDDVLALA
                     GGDASRVSGMLSTVPSAMAALLADPELPLRPHTLLYAGEALTAGLVRDTRAALPGCRVL
                     NVYGPTEATVYATMAEPSPDDPAQPAIGNPLRNVTAHILDANLRPLPPGSPGELYLAGE
                     LHLARGYLNRPGLTAERFVASPFGPPGSRMYRTGDLARQRADGSVEYLGRSDDQVKIR"
     aSModule        complement(290..2896)
                     /domains="nrpspksdomains_H181DRAFT_RS28170_Condensation_LCL
                     .1"
                     /domains="nrpspksdomains_H181DRAFT_RS28170_AMP-binding.1"
                     /incomplete
                     /locus_tags="H181DRAFT_RS28170"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(410..502)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0010"
                     /evalue="5.30E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="849"
                     /protein_start="818"
                     /score="41.1"
                     /tool="antismash"
                     /translation="PPGSPGELYLAGELHLARGYLNRPGLTAERF"
     CDS_motif       complement(992..1048)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0009"
                     /evalue="2.60E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="655"
                     /protein_start="636"
                     /score="26.1"
                     /tool="antismash"
                     /translation="AYAIFTSGSTGRPKAVVVP"
     CDS_motif       complement(1259..1300)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0008"
                     /evalue="1.60E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="566"
                     /protein_start="552"
                     /score="21.1"
                     /tool="antismash"
                     /translation="WAVLKAGAAYVPLD"
     CDS_motif       complement(1601..1672)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0007"
                     /evalue="7.50E+00"
                     /label="C2_starter_024-063"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="452"
                     /protein_start="428"
                     /score="-0.6"
                     /tool="antismash"
                     /translation="DLFDHATVRALADRLVAVLRQAVA"
     CDS_motif       complement(1835..1954)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0006"
                     /evalue="9.90E-24"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="374"
                     /protein_start="334"
                     /score="75.8"
                     /tool="antismash"
                     /translation="RESDLAAYAHQDVPFERLVEVLNPQRSLSRNPLVQVMLAL"
     aSDomain        complement(1991..2896)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H181DRAFT_RS28170_Condensation_L
                     CL.1"
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.00E-119"
                     /label="H181DRAFT_RS28170_Condensation_LCL.1"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="322"
                     /protein_start="20"
                     /score="390.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIAGDG"
                     /tool="antismash"
                     /translation="AQIPLSPAQWRLWFLNQMEGPSPTYNIPYALRLSGRLDEDALRAA
                     LDDVVARHESLRTLFPDEGGDPRQHIVPADRARVTVTAVHTTEDGLAAALTAAGREGFD
                     LAAELPLRVHLLRTGATECVLLLNLHHIAGDGWSMAPLARDLMTAYEARCAGGAPEWAP
                     LPVQYADYALWQRELLGHESDPGSELSRQLGHWRERLAELPAEIALPTDRPRPPVAGYD
                     GALVRFRVSEELHAGLASLARESSATLFMVVQAGLAALLSRLGAGPDVAVGSPVAGRTD
                     EALDDLVGFFVNTLVLRTDVS"
     CDS_motif       complement(2003..2092)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0005"
                     /evalue="4.10E-16"
                     /label="C5_LCL_267-296"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="318"
                     /protein_start="288"
                     /score="51.3"
                     /tool="antismash"
                     /translation="DVAVGSPVAGRTDEALDDLVGFFVNTLVLR"
     CDS_motif       complement(2150..2194)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0004"
                     /evalue="4.30E+01"
                     /label="C3_dual_135-156"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="269"
                     /protein_start="254"
                     /score="-2.3"
                     /tool="antismash"
                     /translation="HAGLASLARESSATL"
     CDS_motif       complement(2360..2398)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0003"
                     /evalue="1.70E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="199"
                     /protein_start="186"
                     /score="20.4"
                     /tool="antismash"
                     /translation="QYADYALWQRELL"
     CDS_motif       complement(2447..2512)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0002"
                     /evalue="9.10E-11"
                     /label="C3_LCL_132-143"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="170"
                     /protein_start="148"
                     /score="34.2"
                     /tool="antismash"
                     /translation="LHHIAGDGWSMAPLARDLMTAY"
     CDS_motif       complement(2714..2830)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_H181DRAFT_RS28170_0001"
                     /evalue="3.90E-20"
                     /label="C2_LCL_024-062"
                     /locus_tag="H181DRAFT_RS28170"
                     /protein_end="81"
                     /protein_start="42"
                     /score="64.3"
                     /tool="antismash"
                     /translation="PTYNIPYALRLSGRLDEDALRAALDDVVARHESLRTLFP"
ORIGIN
        1 cacccgagaa ctccggcacc ggcaaagcag cccggtccaa cttcccgttc accgtcaacg
       61 gcaaccgctc cagcaccacg aacgccgacg gcacgaggtg ccccggcagc gcgtcgacgg
      121 cgacgtcgcg cagctcgtcc gccgtgactc cgccggacga ggcgggcacc acgtaggcgg
      181 ccaggcgctg ttcgccttcg tggtcgcgga gcgaggtgac gaggcattcg cggacggtgg
      241 gcagcgcggt gagcgcggtc tcgatctccc ggggctcgat gcggaatccg cggatcttga
      301 cctggtcgtc tgagcggccg aggtactcga cggatccgtc ggccctctgc cgggccaggt
      361 cgccggtgcg gtacatgcgg ctgccgggcg gcccgaacgg gctcgccacg aagcgctccg
      421 ctgtgagtcc cgggcggttc aggtagccgc gggccaggtg gagttcgccc gcgaggtaga
      481 gctctcccgg tgaacccggg ggcagcggcc gcaggttggc gtccaggatg tgcgcggtga
      541 cgttgcgcag ggggttgccg atcgcgggct gcgccggatc gtcgggcgac ggttcggcca
      601 tcgtcgcgta gacggtggcc tcggtgggcc cgtagacgtt gaggacgcgg cagccgggca
      661 gggccgcacg ggtgtcccgc accagacccg ccgtcagcgc ctcgcccgcg tacagcaacg
      721 tgtgcgggcg cagcggtagt tcggggtcgg cgagcagggc cgccatggcg gacgggacgg
      781 tgctgagcat gccggagacg cgggaggcgt cgccgccggc cagcgcgagg acgtcgtcct
      841 cgaggtcgac gcggccgccg cagagcagcg gcgagaagag ctcgaagacg gacacgtcga
      901 agctgaggga ggtcgtggcg gtgacggctg ccaggccgtc ggcgccgaac tcgccttccg
      961 cccaggcggc caggtcggcc acgttgcgct gcgggacgac gacggccttg gggcgcccgg
     1021 tcgagccgga cgtgaagatc gcgtacgcgg cgttgtccgg ggtgagcggc cgtacgcgtt
     1081 cgccgtcgcc gaggtcgccg tcggggtacg ccgcgacggc ctcgcgcacg ccggggtcgt
     1141 cgagggtgat gcggcgggcc gcgtcgggaa gccgggcagc gccgtcgcgg gtcgtcagga
     1201 cgtacgccgg tgcggcgtcg tcgagcatca gcgtgagccg ctgggccggg tagtcgaggt
     1261 ccagcggcac gtaggcggcg ccggccttga gcacggccca tacggccacg aggagttccg
     1321 gggtgcgcgg catcgcgatg ccgacgaggt cctcgccgcc gacgccggcg tccaccaggt
     1381 ggcgggccaa ctggttggcc ctggcgttga gttcggcgta gcgggtgtcg gtgcccgcgt
     1441 gccgtacggc gaccgcgtcg ggtgtgcggg cggtctgggc ctccagcagc tccggcagcg
     1501 tggcgtcgac gggcccggcc gccgtgtcgt tccactgctc aaggcggctg tactcggccg
     1561 ggctgagcag gtcgacggcg ccgacgggcc ggtccgggtc cgcgacggcc tgccgcagca
     1621 ccgcgacgag gcggtcggcg agggcgcgga cagtggcgtg gtcgaacagg tcggtgctgt
     1681 actccaggac tccgtcgatg ccgccctccg tgccgtccgc tccgaggctc tcgcgcaggt
     1741 ggaaggagag gtcgaacttg gagatgccga cgtcgagcgg ttcgggacgc acccggagtc
     1801 cgggcagggc cgtctcggta cgcggcatgt tctgcagggc gagcatcacc tgcaccagcg
     1861 ggttccggga cagtgagcgc tgcgggttca gcacctccac cagacgctcg aacggcacgt
     1921 cctgatgggc gtacgccgcc aggtcgctct cgcgcacccg ccccagcagc tcccggaacg
     1981 acgggtcgcc cgagacgtcc gtgcgcagca ccagcgtgtt cacgaagaag ccgacgagat
     2041 cgtccagcgc ctcatcggta cgccccgcca cgggcgaccc caccgcgacg tccgggcccg
     2101 cgcccagccg cgacagcagc gcggccaggc ccgcctgcac caccatgaac agcgtcgccg
     2161 acgactcgcg cgccagcgag gccaggccgg cgtggagctc ctcgctcacg cggaagcgga
     2221 cgagggctcc gtcgtagccc gccacgggcg gacgggggcg gtcggtgggc agcgcgatct
     2281 cggcgggcag ctcggcgagg cgttcgcgcc agtgtccgag ctggcgggag agttcgctgc
     2341 ccgggtcgga ctcgtgcccg agcagctccc gctgccagag ggcgtagtcg gcgtactgca
     2401 cgggcagcgg cgcccactcg ggcgctcctc ccgcgcaccg cgcctcgtag gccgtcatca
     2461 ggtcacgggc gagcggggcc atggaccagc cgtcgcccgc gatgtggtgc aggttcagca
     2521 gcagcacgca ctcggtggcg ccggtgcgca acaggtggac gcgaaggggc agttcggcgg
     2581 ccaggtcgaa tccctcgcgt cccgccgcgg tgagggcggc ggcgaggccg tcctccgtcg
     2641 tgtgcacggc cgtcaccgtg acgcgggccc ggtcggcggg cacgatgtgc tggcgcgggt
     2701 ctcctccctc gtcggggaag agggtgcgca ggctctcgtg ccgggcgacc acgtcgtcca
     2761 gggcggcgcg cagcgcgtcc tcgtcgaggc gtcccgacag ccgcagggcg taggggatgt
     2821 tgtaggtggg actgggcccc tccatctggt tgaggaacca caggcgccac tgcgcgggtg
     2881 acagcgggat ctgcgcggga cgttcggccg gacgcagcgg cgtacgcgcc gcggccgcac
     2941 cctccagagc acgcaccaga cccgccggag tcggactgtc gaacaacgcc cgcac
//
