LOCUS       NZ_FMHJ01000005        10405 bp    DNA     linear   CON 28-JUL-2024
DEFINITION  Streptomyces sp. WMMB 714, whole genome shotgun sequence.
ACCESSION   NZ_FMHJ01000005
VERSION     NZ_FMHJ01000005.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. WMMB 714
  ORGANISM  Streptomyces sp. WMMB 714
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,Neha. and Submissions,Spin.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2016) DOE - JOINT GENOME INSTITUTE, 2800 Mitchell
            Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900090125.1-RS_2024_07_28
            Annotation Date                   :: 07/28/2024 02:17:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,656
            CDSs (total)                      :: 5,578
            Genes (coding)                    :: 5,496
            CDSs (with protein)               :: 5,496
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 5, 4 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 15 of 82
            Pseudo Genes (incomplete)         :: 72 of 82
            Pseudo Genes (internal stop)      :: 4 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 420916
            Orig. end                         :: 431321
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FMHJ01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="2"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(8..22)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: weak, score: 20.08"
                     /tool="antismash"
     gene            complement(88..312)
                     /locus_tag="H181DRAFT_RS11935"
                     /old_locus_tag="H181DRAFT_02405"
     CDS             complement(88..312)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H181DRAFT_RS11935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H181DRAFT_02405"
                     /product="hypothetical protein"
                     /protein_id="WP_139131698.1"
                     /transl_table=11
                     /translation="MKTSTARSARSVLCLVAWAACLPCLSLRTAWVSGSHIGIPEGSPL
                     REGGGMLAAANALGVVLDSFVVVRPHGPP"
     gene            complement(422..1192)
                     /locus_tag="H181DRAFT_RS11940"
                     /old_locus_tag="H181DRAFT_02406"
     CDS             complement(422..1192)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1195: IclR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381154.1"
                     /locus_tag="H181DRAFT_RS11940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_02406"
                     /product="IclR family transcriptional regulator"
                     /protein_id="WP_045864305.1"
                     /transl_table=11
                     /translation="MSAGETGASQVKSAVRTVELLEFFAGRPGMHSLASVQEAVGYPKS
                     SLYMLLRTLVDLGWVETDSTGTRYGIGVRALLVGTSYIDGDEAVAAARPTLDRLSEDTT
                     ETIHLARLDGTNVVYLATRQSQHYLRPFTRVGRRLPAHSTSLGKALLATYDDEQVREML
                     PEKLPALTEHTVTDREALIRELALVREQGYAVDREENTLGLRCFGVAIPYRTPARDAVS
                     CSVPVARLTPAHEQMIRDALFDARDRLTLATRRL"
     gene            1309..2844
                     /locus_tag="H181DRAFT_RS11945"
                     /old_locus_tag="H181DRAFT_02407"
     CDS             1309..2844
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013004607.1"
                     /locus_tag="H181DRAFT_RS11945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_02407"
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="WP_254666810.1"
                     /sec_met_domain="Aldedh (E-value: 2.5e-45, bitscore: 149.5,
                     seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVAAPVWSVDPRTGQQREQVGTEATAVDVDAAVRAAHGTLEPLA
                     DRTVRAALLRAAAEQLGAASEEIITTADAETALGRPRLPGELARTQAQLRAFADEIEDG
                     TYLGITVNHADPDATPPCPDLRRYRIPLGVVAVYAASNFPLAFSVPGGDTASALAAGCP
                     VVVKAHPDHPATSELTAAALRRAAAEVGLPEDVLTVVHGFEAGVELIKHPLVAAAGFTG
                     SVRGGRALFDAAAARPAPIPFHGELGSLNPVVVTAAAAAERAEEIGAGLAGSMTMGTGQ
                     FCTKPGFVLAPEGEDGDRLLSALREKVAEAAQGPLLDSRMRESFLEGISARTELEGVEA
                     PVLAGAGDDEHTVGAGVLTVPAGKVGQPQYELLLEECFGPVTVVARYRDDDEVSAVLSR
                     LPGNLTATVQLSGGEAAGDGGRGARLLAALTPLAGRVLVNGWPTGVAVAPAQHHGGPYP
                     ATTSTSTSVGATAIERWLRPVAYQDAPDALLPAELRESNPLGLPRRVDGRWER"
     gene            complement(3016..3861)
                     /locus_tag="H181DRAFT_RS11950"
                     /old_locus_tag="H181DRAFT_02408"
     CDS             complement(3016..3861)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090397.1"
                     /locus_tag="H181DRAFT_RS11950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_02408"
                     /product="DUF1989 domain-containing protein"
                     /protein_id="WP_045864307.1"
                     /transl_table=11
                     /translation="MAGTAAAYRASEGGPLDVDRAFYGRAASALDGRELVERFTVPIRS
                     GRAWEVPAGHVCRLVTVDGPQVGDLNVWNRHDPRERLWASRTRQLQRAHVSTYDRLWST
                     LPFLRPLLTITSDTLAGYGRDAEGGRVHDLLGTRCDPYVNRMLTGEDFDFHCHSNLVRA
                     VLPYGLTEFDVHDVLNVFQCTGLNDDDQYFMKDCPARPGDHLEFFAELDLLCALSTCPG
                     GDLSVPMWGPGSDVDPVEFCHPIGVEVYRLAPELLEGWTPPERAAYRNMHGMSRPEWRP
                     "
     gene            complement(4007..4906)
                     /gene="thpD"
                     /locus_tag="H181DRAFT_RS11955"
                     /old_locus_tag="H181DRAFT_02409"
     CDS             complement(4007..4906)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358305.1"
                     /locus_tag="H181DRAFT_RS11955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_02409"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_045864308.1"
                     /sec_met_domain="PhyH (E-value: 3e-39, bitscore: 130.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTTEVATPRMDPVVWSEPGAPGPIEAAVLA
                     DYERDGFLSVDQLITPDEVDMYRQELNRLTTDADVRADERSIIEPKSDEIRTVFEVHKI
                     SELFADLVKDPRVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLP
                     HMRTVSVSIALTRNYDTNGGLMIMPGSHNTFLGCAGETPKDNYKKSLQMQDAGIPSDES
                     LTKFADAHGIKLFTGEPGSAVWFDCNCMHGSGDNITPYPRSNVFIVFNSVENEAVEPFA
                     APVRRPEFIGARDFTPVR"
     gene            complement(5001..5405)
                     /locus_tag="H181DRAFT_RS11960"
                     /old_locus_tag="H181DRAFT_02410"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907220.1"
                     /locus_tag="H181DRAFT_RS11960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_02410"
                     /product="ectoine synthase"
                     /protein_id="WP_045864309.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.2e-54, bitscore:
                     178.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSIKDIEGTDRHIKSASGTWESKRIVLAKERVGFSLHETTLY
                     AGTETSMWYANHIEAVLCVEGEAELTNDETGEKHLITPGTMYLLDGHEKHTMRPKTDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPAED"
     gene            complement(5482..6750)
                     /gene="ectB"
                     /locus_tag="H181DRAFT_RS11965"
                     /old_locus_tag="H181DRAFT_02411"
     CDS             complement(5482..6750)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-365). E-value: 6.2e-75.
                     Score: 244.2. Matches aSDomain:
                     nrpspksdomains_H181DRAFT_RS11965_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="H181DRAFT_RS11965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_02411"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_045864310.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.2e-78, bitscore:
                     257.9, seeds: 13, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.2e-05, bitscore:
                     19.4, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRSWPTIFDRAQGSHMYDEDGHTY
                     LDFFAGAGTLNYGHNNPVLKRALLDYLERDGVVHGLDMSTTAKRAFLETFHEKILKPRD
                     MNHKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDDYLGGTTEDFVWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRDLADLCERHDMLLIVDDIQMGCGRTGAFFSFEEAGIKPDIITLSKSIGGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTATAAVETYWTDSQMEKQTLSRGEQVEAVLR
                     AISEEHTEDVADFRGRGLVWGLEFHDKDRAGAVAKRCFDLGLLVETSGPQSEVVKLLPS
                     LTITPEELDEGLRVLARAVRETA"
     aSDomain        complement(5656..6663)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H181DRAFT_RS11965_Aminotran_3.1"
                     /evalue="6.20E-75"
                     /label="H181DRAFT_RS11965_Aminotran_3.1"
                     /locus_tag="H181DRAFT_RS11965"
                     /protein_end="365"
                     /protein_start="29"
                     /score="244.2"
                     /tool="antismash"
                     /translation="DRAQGSHMYDEDGHTYLDFFAGAGTLNYGHNNPVLKRALLDYLER
                     DGVVHGLDMSTTAKRAFLETFHEKILKPRDMNHKVMFPGPTGTNAVEAALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDDYLGGTTEDFVWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRDLADLCERHDMLLIVDDIQMGCGRT
                     GAFFSFEEAGIKPDIITLSKSIGGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFVT
                     ATAAVETYWTDSQMEKQTLSRGEQVEAVLRAISEEHTEDVADFRGRGLVWGLEFH"
     gene            complement(6872..7399)
                     /gene="ectA"
                     /locus_tag="H181DRAFT_RS11970"
                     /old_locus_tag="H181DRAFT_02412"
     CDS             complement(6872..7399)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180701.1"
                     /locus_tag="H181DRAFT_RS11970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_02412"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_045864311.1"
                     /transl_table=11
                     /translation="MPEGLTLDAPRPEDGAALWRITRDSRTLDLNSSYSYLLWCRDFAG
                     TSVVARGADGEPVGFITGYVRPRQPRTLLVWQVAVDEAARGRGLAAAMLDGLAARVARE
                     AGIDGIETTVTPDNTPSNRLFTSFAARHGAPVEKEVLFDAGDFPESGHEPEVLYRIGPV
                     ASLSGLGPLTTR"
     misc_feature    complement(7397..7411)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.45"
                     /tool="antismash"
     misc_feature    complement(7451..7467)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     misc_feature    complement(7607..7622)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7653..7671
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.79"
                     /tool="antismash"
     gene            7887..8978
                     /locus_tag="H181DRAFT_RS11975"
                     /old_locus_tag="H181DRAFT_02413"
     CDS             7887..8978
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014141820.1"
                     /locus_tag="H181DRAFT_RS11975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_02413"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_045864312.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 1.2e-15, bitscore:
                     52.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDGGILHIKGRVLAGPEDVRDELWVVGGHITYERPPGTVPDTGV
                     IEGWVLPGLVDAHCHVGLGSKGPVDESATEKQAITEREAGALLLRDAGSPSDTRWVDDR
                     ADLPRIIRAGRHIARTKRYIRNFAHEIEPGDLAAYTRREARRGDGWVKLVGDWIDREEG
                     DLAPCWPRWAVEEAIAAAHEEGARVTAHCFAESSLRDLVEAGIDCVEHATGLTEETVPL
                     FAERGVAIVPTLVNTATFPQLAEGGKKYPRWAAHMRQLHERRYETVRAAYEAGIPVFTG
                     TDAGGSLPHGLVSAEVGELVKAGIPAADALSAAVWGARRWLGRPGLEEGAPADLVVYAE
                     DPRADVGVLGSPRRVVLRGEVVH"
     misc_feature    complement(9825..9839)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.93"
                     /tool="antismash"
ORIGIN
        1 ccccgcggcc cacatggcgt gcacgtcgcc cggggcagca gggcggcgag ggagccgaga
       61 agcgcgtgag aggggcgcac ggccggctca cggcggcccg tggggtcgga cgacgacgaa
      121 ggagtccagc accacgccca gcgcgttcgc cgcggcgagc atcccgccgc cttcgcgcag
      181 cgggcttccc tccgggattc cgatgtggct gccggacacc caggcggtcc tgagcgagag
      241 acacggcagg cacgcggccc atgcgaccag gcacagaacg gagcgtgcgc ttcgcgcggt
      301 cgaggtcttc atgcgctcca ccctcgcgcc gcgccggggc gggggcgtcc tgcccgacgg
      361 cgaacggcct ccgccgggcg gcggagaccc cctcgtcccg ggtgcggagc cggccggccc
      421 gtcagaggcg cctggtggcc agcgtcagcc ggtcccgtgc gtcgaacagc gcgtccctga
      481 tcatctgctc gtgcgcgggc gtcagccgtg cgacggggac cgagcagctg acggcgtccc
      541 gcgcgggcgt ccggtagggg atggcaacgc cgaagcagcg cagccccagc gtgttctcct
      601 cgcggtcgac ggcgtagccc tgctcgcgta ccagcgcgag ctcccggatc agcgcctcgc
      661 ggtcggtgac ggtgtgctcc gtgagggccg gcagcttctc cggcagcatc tcgcggacct
      721 gctcgtcgtc gtacgtggcc agcagcgcct tgccgagcga cgtggagtgc gcgggcagcc
      781 gccgcccgac gcgggtgaag ggccgcaggt agtgctgcga ctggcgcgtg gcgaggtaga
      841 cgacgttcgt gccgtcgagc ctggcgaggt ggatggtctc ggtggtgtcc tcggagagcc
      901 ggtccagcgt cgggcgggcg gcggccacgg cctcgtcgcc gtcgatgtag gaggttccga
      961 ccagcagcgc ccgcacgccg atgccgtagc gtgtgcccgt cgagtccgtc tcgacccagc
     1021 ccagatcgac gagcgtgcga agaagcatgt acagactgga cttggggtac ccgacggctt
     1081 cctgcacgga ggcgagggag tgcatgccgg gccgtccggc gaagaactcc agcagctcca
     1141 ccgtgcggac agcggacttg acctgcgacg cacccgtctc accggctgac atcgaccttg
     1201 accccctcgt cgcggcactc ttagggtccc cgcagtggtt cactgacaga gacggtgttc
     1261 agcatatcga acgacctctc tgcggagaca agcaggcggc aggaggaaat cacggtggca
     1321 gcaccagtgt ggagcgtcga ccccaggacc ggacagcagc gcgagcaggt cggcacggag
     1381 gccacagccg tcgacgtcga cgcggcagta cgcgccgcgc acggcaccct cgagccgctc
     1441 gccgaccgca ccgtgcgcgc cgcgctgctc cgcgccgccg cggaacaact gggcgcggcg
     1501 agcgaggaga tcatcacgac ggcggacgcg gagaccgccc tcgggcggcc gcggctcccg
     1561 ggcgaactgg cccgcaccca ggcccagttg cgggccttcg ccgacgagat cgaggacggc
     1621 acctacctcg gcatcaccgt caaccacgcc gaccccgacg cgactccgcc ctgccccgac
     1681 ctgcgccgct accggatccc gctcggcgtc gtggcggtct atgccgcctc gaacttcccg
     1741 ctggccttct ccgtgcccgg cggcgacacc gccagcgcgc tcgccgcggg ctgccccgtc
     1801 gtcgtcaagg cccacccgga ccacccggcg acgtccgagc tgacggcggc ggcgttgcgc
     1861 cgcgcggccg ccgaggtggg gctgccggag gacgtgctca ccgtggtgca cgggttcgag
     1921 gcgggcgtgg agctcatcaa gcaccccctc gtcgcggcgg cggggttcac cgggtccgtc
     1981 cgcggcggcc gcgccctctt cgacgccgct gcggccaggc cggcgcccat ccccttccac
     2041 ggcgaactcg gctccctgaa ccccgtggtg gtcacggccg ccgcggcggc cgagcgggcg
     2101 gaggagatcg gtgcggggct cgcaggttcc atgacgatgg gcaccggcca gttctgcacg
     2161 aagcccggat tcgtcctcgc cccggagggt gaggacggcg accggctgct gtccgccctg
     2221 cgggagaagg tcgccgaggc ggcccaaggg cccttgctgg acagccggat gcgcgagtcc
     2281 ttcctggaag gcatcagcgc acggacggag ttggagggcg tcgaggctcc cgtgctcgcg
     2341 ggcgcgggcg acgacgagca caccgtcggt gccggcgttc tcaccgttcc cgccgggaag
     2401 gtcgggcagc cgcagtacga gttgctgctg gaggagtgct tcggcccggt caccgtcgtc
     2461 gcgcgctacc gggacgacga cgaggtctcc gcggtgctct cgcggctgcc cggcaacctg
     2521 accgccaccg tccagctcag cggaggcgaa gcggccgggg acggcggccg cggcgcgcgc
     2581 ctgctggcgg cgctgacccc gctggcgggc cgcgtccttg tcaacggctg gcccacgggc
     2641 gtcgccgtgg cgcccgcaca gcaccacggc ggcccctacc cggccaccac gtccacctcg
     2701 acctccgtgg gcgccaccgc gatcgagcgc tggctccgtc ccgtcgccta ccaggacgcg
     2761 ccggacgcgc tgctgccggc cgaactgcgc gagtccaacc cgctggggct gccgcggcgc
     2821 gtcgacgggc gctgggagcg ctgagaggcg cggtcggcgc gtggcgcggc ctgatccgga
     2881 aggaccccga ggccctggac aggccccgga caagccccgg acaagccccg gacaggcggc
     2941 gtcccgcggg aggccgccgc gcttgttccg ttcggcgatg gtgttcgggg cgctcgcgcc
     3001 tggaaagggg agggttcagg gccgccactc gggacggctc atgccgtgca tgttgcggta
     3061 cgcggcccgt tccgggggcg tccagccctc cagcagctcc ggagcgaggc ggtagacctc
     3121 gacgccgatc ggatggcaga actcgacggg gtcgacgtcg gagccaggcc cccacatggg
     3181 aaccgacaga tcgccgcccg ggcacgtcga cagcgcgcac agcaggtcga gttcggcgaa
     3241 gaactccaga tggtcgccgg ggcgcgcggg gcagtccttc atgaagtact ggtcgtcgtc
     3301 gttgagtccg gtgcactgga agacgttgag gacgtcgtgc acgtcgaact ccgtgagccc
     3361 gtacgggagt acggcgcgca ccaggttcga gtggcagtgg aagtcgaagt cctcgccggt
     3421 cagcatgcgg ttgacgtacg ggtcgcagcg cgtgccgagc aggtcgtgga ccctgccgcc
     3481 ctccgcgtcc ctcccgtatc cggcgagggt gtcggaggtg atcgtcagca gcggccgcag
     3541 gaacggcagc gtcgaccaca gccggtcgta ggtgctcacg tgcgcccgct gcaactgcct
     3601 tgtgcgcgag gcccacagcc gctcgcgggg atcgtgcctg ttccacacgt tgaggtcgcc
     3661 gacctggggg ccgtccaccg tgaccaggcg gcacacgtgc cccgcgggga cctcccatgc
     3721 ccggccggag cggatcggca cggtgaagcg ctccacgagc tcccgtccgt ccagcgcgga
     3781 ggccgcgcgt ccgtagaacg cgcggtcgac gtcgaggggg ccgccctcgg atgcccggta
     3841 tgcggctgcg gtgcctgcca tgcgtgcgta cgctccctcg ctgttgtgct gtcgtgctgt
     3901 ccgagtcgcc tccgccgatc tcagcagagc ggcggccgcg cggagaaggg ggaggccgcc
     3961 atgcggcctc ccccttctcc agcgggatca ggtgtgacgt cgtgtgtcac ctgacgggcg
     4021 tgaagtcccg cgccccgatg aactcggggc gtcgtaccgg tgccgcgaag ggctcgacgg
     4081 cctcgttctc cacgctgttg aagacgatga acacgttgct cctggggtac ggagtgatgt
     4141 tgtcaccgct tccgtgcatg cagttgcagt cgaaccagac cgccgaaccc ggctcgcccg
     4201 tgaagagctt gatgccgtgt gcgtcggcga acttggtcag ggactcgtcc gagggaatcc
     4261 cggcgtcctg catctgcagc gacttcttgt agttgtcctt cggcgtctcg cccgcgcagc
     4321 cgaggaaggt gttgtgcgac cccggcatga tcatcaagcc gccgttggtg tcgtagttgc
     4381 gggtcagcgc gatcgagacc gagacggtcc gcatgtgggg caggccgtct tccgcgtgcc
     4441 aggtctcgaa gtcggagtgc cagtagaagc ccgtggcccc gaagcccggc ttgacgttga
     4501 tcctggactg gtggacgtag acgtccgagc ccaggatctg ccgtgcccgg cccactaccc
     4561 gcgggtcctt caccaggtcg gcgaagagct cgctgatctt gtgcacctcg aacacggtgc
     4621 ggatctcgtc cgacttgggc tcgatgatcg agcgctcgtc ggcacggaca tccgcatccg
     4681 tggtgagccg gttcagttcc tgccggtaca tgtcgacctc atcgggagtg atgagctggt
     4741 cgaccgaaag gaatccgtcc cgctcgtagt ccgccagcac cgcggcctcg atgggccccg
     4801 gggcacccgg ctcggaccac accaccgggt ccatgcgtgg tgtggccacc tcggtggttc
     4861 cgcgagtcgg gtacaggtcg gcggttcgct cgggtgcggt ggtcatggtg ttgccttcct
     4921 ctcctctcgt acggccctgt attggtgggc gagcggcctg atgcggccga atcggcctgt
     4981 ggcggccggg ctttggaagc tcagtcctcg gcgggttcgg tgagaagcgg gtagacgccg
     5041 ttctcgtcgt gatcctcccg gccggtgacg ggcgggttga agacacagat gcaccggaag
     5101 tcggtcttgg ggcgcatggt gtgcttctcg tgcccgtcga ggaggtacat ggtgccgggg
     5161 gtgatcaggt gcttctcacc cgtctcgtcg ttggtgagtt ccgcctcgcc ctcgacgcag
     5221 agtacggctt cgatgtggtt tgcgtaccac atcgacgtct ccgtacccgc gtagagcgtg
     5281 gtctcgtgca gggagaagcc gactcgctcc ttggccagca cgatccgctt gctctcccag
     5341 gtaccggatg cggacttgat gtgtcggtcg gtgccttcga tgtccttgat ggaacgaaca
     5401 atcacgttct gtcttgccct tctctctcat tacggggtcg gcccgccccg gacgcaaccg
     5461 gggcgggccg tgaacgggaa gttacgcggt ttcgcggact gcgcgcgcca gcacgcgcag
     5521 cccctcgtcc agctcctcgg gagtgatcgt cagggacggc aggagcttga cgacctcgct
     5581 ctgcggaccc gaggtctcca cgagcagacc caggtcgaag cagcgcttgg cgacggcacc
     5641 ggcgcgatcc ttgtcgtgga actccaggcc ccacacgagg ccgcggccgc ggaagtcggc
     5701 cacgtcctcg gtgtgttcct cggaaatcgc gcgcaaaacc gcttccacct gctcaccgcg
     5761 ggagagcgtc tgcttctcca tctggctgtc ggtccagtag gtctccaccg cggccgtggc
     5821 cgtcacgaag gccgggttgt tgccgcggaa ggtgccgttg tgctcgccgg gctcccagat
     5881 gtccagctcg ggcttgaaca gggtgagcgc cagcggcagt ccgtagccgc cgatggactt
     5941 cgacagggtg atgatgtccg gcttgatgcc cgcctcctcg aaggagaaga aggcgccggt
     6001 gcggccgcag cccatctgga tgtcgtcgac gatgagcagc atgtcgtgcc gctcgcacag
     6061 gtcggccagg tcgcgcagcc actcggcgcg cgcgacgttg atgccgccct cgccctgcac
     6121 ggtctcgacg atcaccgcgg cgggcttgtt gaggccggag ccctggtcct ccagcagccg
     6181 ctcgaaccag acgaagtcct cggtcgtgcc gccgaggtag tcgtcgaacg gcatcggggt
     6241 gccgtggacg agcgggatgc cggcgccggc gcgcttgaag gcgttgccgg tgacggcgag
     6301 cgagcccagc gacatgccgt ggaaggcgtt ggtgaaggag acgatcgact cgcggccctt
     6361 gaccttgcgg gcgagcttca gcgccgcctc gacggcgttg gtgccggtcg ggccggggaa
     6421 catcaccttg tggttcatgt cgcggggctt gaggatcttc tcgtggaagg tctccaggaa
     6481 ggcccgcttg gcggtcgtgc tcatgtccaa cccgtgcacg acgccgtcac gttcgaggta
     6541 gtcgagcagc gcccgtttga gaacggggtt gttgtggccg tagttgagcg ttcccgcacc
     6601 ggcgaagaag tccaggtagg tgtggccgtc ttcgtcgtac atgtgcgagc cctgggcccg
     6661 gtcgaagatc gtgggccagc tgcggcagta gctgcggact tccgactcga cggtctcgaa
     6721 gacgctgagg tccggctggg tgatggtcac agcatgctcc tgggagtgag ttgagtgctg
     6781 agaagtgctt cttgcggatg tgccttgcga agttcagggg ttgcggatct gttcgaagtt
     6841 cgggacgcga agcggtatgc gcggggggcg ctcagcgcgt ggtgagcggc ccgaggccgg
     6901 acagcgacgc gaccggcccg atgcggtaca gcacttcggg ttcgtgaccg ctctcgggga
     6961 agtcgcccgc gtcgaagagc acctccttct ccacgggggc gccgtggcgc gccgcgaaag
     7021 aggtgaagag gcggttcgag ggcgtgttgt ccggggtgac ggtggtctcg atcccgtcga
     7081 tccccgcttc ccgtgcgacg cgtgcggcga gcccgtcgag catcgccgcg gccagcccgc
     7141 ggcctcgtgc cgcctcgtcg acggcgacct gccagacgag gagcgtccgg ggctgccggg
     7201 ggcgcacgta gccggtgatg aagccgaccg gttcgccgtc cgcgccccgt gctaccacgg
     7261 aggtccctgc gaagtcgcgg caccacagga gatagctgta ggaggagttg aggtcgagcg
     7321 tccgtgagtc gcgggtgatg cgccacagcg ccgccccgtc ctcaggtcgc ggagcgtcga
     7381 gtgtcagtcc ctccggcatt tccaggaaaa tcctgtgggc acgtgcatgg tctgcttgtg
     7441 cggcggtcat gggtattaaa tttacccagc ggatgccaaa aacgcacggc gggccggggt
     7501 tacgcgagaa ggggtccctg tgttatcgcg cgcaggcgcg caggtgagat gggcggcgcc
     7561 gttatgcccc gacttaaagg gataatcccg gaggatgcgg gcggtctgtg gagtctgtca
     7621 catcctcgaa acgaccttgt gaggtcctcg aattacgtca gggtaaccat agaaaattcg
     7681 agtgtttaag cgatcggaaa gcgggaacaa gaatacgggc ggctacgctc gatgaattcc
     7741 gggaatgcat tctcctccgt tgagttcaat gtcgcatctc caggcccgcg agtgcaatgg
     7801 gatcgccacg ttccgtatgc ggtcctgagc agccggaaat gccggccggg tatgaacgcg
     7861 ggggagcggc tcgtaggctg cgggccatga gcgacggcgg gattctccac atcaaggggc
     7921 gcgtgctggc cgggcccgag gacgtccggg acgagctgtg ggtggtgggc gggcacatca
     7981 cctacgagcg ccccccgggc acggttcctg acacgggcgt catcgagggc tgggtgctgc
     8041 ccgggctcgt ggacgcccac tgccatgtgg gcctcggctc caagggcccc gtcgacgagt
     8101 cggccacgga gaagcaggcg atcacggagc gggaggccgg tgcgctgctg ctgcgcgacg
     8161 cgggctcccc ctcggacacc cgctgggtcg acgaccgtgc ggatctgccg cggatcatcc
     8221 gcgccggcag gcacatcgcc cgcacgaagc gctacatccg caacttcgcg cacgagatcg
     8281 aacccggcga cctcgccgcc tacacccggc gtgaggcccg ccgcggcgac ggctgggtga
     8341 agctcgtggg ggactggatc gaccgtgagg agggcgacct cgccccgtgc tggccgcgct
     8401 gggccgtgga ggaggcgatc gccgccgccc acgaggaggg cgcccgcgtc acggcgcact
     8461 gcttcgcgga aagctcgctc cgcgacctgg tcgaagccgg aatcgactgc gtcgaacacg
     8521 ccacggggct cacggaggag accgtcccgc tcttcgccga gcgcggcgtc gcgatcgtcc
     8581 ccaccctcgt gaacaccgcc acgttcccgc agctcgccga gggaggcaag aagtaccccc
     8641 gctgggccgc gcacatgcgg cagctccacg agcgccgcta cgagacggtc cgcgccgcgt
     8701 acgaggcggg catccccgtc ttcacgggga cggacgccgg cgggtcgctg ccgcacggac
     8761 tcgtctccgc ggaggtcggc gagctggtca aggcgggcat ccccgcggcg gacgccctgt
     8821 cggcggccgt ctggggcgcc cggcgctggc tgggccgccc cgggctggag gagggagccc
     8881 ccgccgacct cgtcgtgtac gcggaggacc cgcgggcgga tgtcggggtg ctgggctccc
     8941 cgcggagggt cgtgctgcgg ggagaggtgg tgcactagcc gtggaggacg gcggagcgcg
     9001 cacgggccgg gagcacgggc ggcgcacgga cgaggccccg cggcggacac cggaggggcc
     9061 ccggcgggcg gacgccgcag ggatcgtctc gtggagcgcc atggagctct cgcgggccgt
     9121 cgcgggccgc gaagtgacgt gcgccgaggt catgcgggca tacctggagc acatcgaacg
     9181 ggtcaatccg cacttctgcg cgatcgtcgg ggcccaggcg ccggagctct ccctggagca
     9241 ggcgcgagag cgggaccggg agctggagcg cggcgagagc cgcggctgga tgcacggctt
     9301 cccctacgcg gtgaaggacc tcgccgaggc gcgcggcctg ccgtggacgg agggttcgcc
     9361 cctgcacagg gaccgcgtcg cggagcggga cgccccgttc gtgcggcgcg tcagggacgc
     9421 cggcgccgtc gtgatcggca agaccaacac ccccgagttc ggcctgggtt cgcagacgta
     9481 caaccaggtg tgggggacga cgggcaacgc ctacgacccc gcgctcacgg cgggcggcag
     9541 cagcggggga gcggcctgcg ccctcgccct gcggatgctg ccggtcgccg acggcagcga
     9601 ctacatgggc tccctgcgca accccgccgc gttcaacaac gtgctcggca tgcgcccgag
     9661 tcagggacgc gttccgggcc ccgggttcac cggtcagccg ggcgtggccg gcccgatggc
     9721 ccgcacggtg acggaccttg cgtggctgct gtcggtgatg gcggggccgc acgaggagtc
     9781 cccgacggcg ttcccggaga gcggcgaacg cttccgggag cccctggaac gcgacttccg
     9841 ggggacccgc gtggcatggg tcggcgactg gcgggggcgg ctggccaccg agccgggagt
     9901 gctggaggtg tgccgctcct cgctggccgt cttcgaggcg ctcgggtgcg aagtcgtgga
     9961 cgccgtaccg gacttcccgg ccgacgagct ctggcggacg ttcctcacct ggcgctggtg
    10021 ggcggccctg gaccggtacg gcctgtacac cgaccccgcc gcgcgggagc ggatgaagcc
    10081 cgaggcggtg tgggagaccg agtggggact ggccctgagc gccctcgacg tcacggaggc
    10141 ggccgcgtcg cgggaccgct ggtacgcggc gctggcggag ttcttccgtg gttacgacta
    10201 catcctcgcc ccggcggcac aggtcttccc cttcgcgaag gagacgaggt ggcccgaggt
    10261 ggtcggcgga cgccgcatgg acacttacca ccgctggatg gagacggtgg tgccctggtc
    10321 gctggccggt gtgccggtga tcgggatgcc ggccgggttc ggccggcagg ggctgcccgc
    10381 gggcctgcag ctcgtgggca gaccg
//
