LOCUS       NZ_FMHJ01000004        11887 bp    DNA     linear   CON 28-JUL-2024
DEFINITION  Streptomyces sp. WMMB 714, whole genome shotgun sequence.
ACCESSION   NZ_FMHJ01000004
VERSION     NZ_FMHJ01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. WMMB 714
  ORGANISM  Streptomyces sp. WMMB 714
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,Neha. and Submissions,Spin.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2016) DOE - JOINT GENOME INSTITUTE, 2800 Mitchell
            Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900090125.1-RS_2024_07_28
            Annotation Date                   :: 07/28/2024 02:17:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,656
            CDSs (total)                      :: 5,578
            Genes (coding)                    :: 5,496
            CDSs (with protein)               :: 5,496
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 5, 4 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 15 of 82
            Pseudo Genes (incomplete)         :: 72 of 82
            Pseudo Genes (internal stop)      :: 4 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 179504
            Orig. end                         :: 191391
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FMHJ01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11887
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11887
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11887
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6887](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6887
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(787..1893)
                     /locus_tag="H181DRAFT_RS08545"
                     /old_locus_tag="H181DRAFT_01725"
     CDS             complement(787..1893)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1178: cobalamin synthesis protein/P47K family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018833994.1"
                     /locus_tag="H181DRAFT_RS08545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_01725"
                     /product="GTP-binding protein"
                     /protein_id="WP_052708504.1"
                     /transl_table=11
                     /translation="MITFIPLTGFLGAGKTTTMTTTALALQEKGHRVAVITNDQGVELV
                     DTQLVRSKLDSVAEVTGGCFCCKFEDLVEAVVALTGSDGVDTVIAEAVGSCTDLQATVV
                     RPLRRYYGDNMVVAPLTTVVDPLRHRAFERAAQRGEPESDLSYLFRQQLTEADVIAVNK
                     LDTITGDQADELLPGLRKQYGDAAVVGYSATTGEGVDTLLAAWEAPAGNQAVDLEVDYD
                     RYAAAEAQLAWMNQEVELSADGEEFDATDWARTVLARLSGWAAEHGAVIGHAKLTVDDG
                     TGEFAKLSLTEAGATPTVDSVLATAVADARAVVNARVACEPDDLDRAVTDAVAAADEAT
                     GARSSATSPVSFKPGYPRPVHRLAPAGA"
     gene            complement(1926..3185)
                     /locus_tag="H181DRAFT_RS08550"
                     /old_locus_tag="H181DRAFT_01726"
     CDS             complement(1926..3185)
                     /EC_number="2.3.1.-"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /locus_tag="H181DRAFT_RS08550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_01726"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_139131680.1"
                     /transl_table=11
                     /translation="MGGSPRVVVSTGQALPRMGWSEELTDLTDAWPYLGPTWLAATEKA
                     LPEAQPWHTVATRARGELAVLPGYVLASPPVVDHEPRTYLGRQAPTGEEVCCGAETDSA
                     RSAEIDELGTEPFFPTLLLGSPLGYRTEPAYNFWTPTLVGTLVEKLVPAAFDAGVRCVL
                     APWIPDRRVGRDLVTALDSAGGHHCFWGYEDFLSLDAPDWEGHLAALPLKKRQRIKADV
                     RKAAAAGITIERADGTGIRPYVERIAELTCLNREKNGAGEEPGHIAGILSALIDEGADV
                     RAYLGRKSGELVASCVTVRKHHRLFPKWAGFDYAAIGERSGVYFALVLDAPVRDAYAEG
                     LRTVEFGAGAHRAKVLRGCTPRAMTTSLLLSDPNLRPRAAAWLDAFGSSRHSAFGDAVP
                     SRPAAPPLLSESSGGCCSAG"
     gene            complement(3215..4465)
                     /locus_tag="H181DRAFT_RS28310"
                     /old_locus_tag="H181DRAFT_01727"
     CDS             complement(3215..4465)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_150234437.1"
                     /locus_tag="H181DRAFT_RS28310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_01727"
                     /product="RCCLKC-tail radical SAM protein"
                     /protein_id="WP_139131681.1"
                     /sec_met_domain="PF04055 (E-value: 2.8e-15, bitscore: 51.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQPRVLVASAFEAEMQPLTCAYAAASLQAHDVETVGWDAHFQPD
                     AFPEGPFDLALVSVQQFEGLERGLSLARELKERFATTVVAFGQYAQMNHREFLKAADAV
                     VMEEPELIGEELSDLARGTRDLHAVPALMTSRGMRPRPPRRRISVAAPARDLFPSLVHY
                     PAHHSPFGLMGNIEASRGCHHKCTYCSVYGAYDGGVAVYEADSVLADALQLADEGVRHF
                     CFIDAEFFNSRTIGAGIVERLADAVGPITFEFTTRVDHVLSYPRELEKLVSLGLRRVTC
                     ALEFPSDRVLRIFDKHIDVDHMRQAVVEARRIGFELYPTFIPFTPWVEYEELLGFEDWL
                     VEADLAHVTDPTALQTRLLLFKGSPLLSSPWMEDVATVDRGFWTEWTHPDRRVEELWQQ
                     RRSDAEDAGRVRCCVKC"
     gene            5001..6887
                     /locus_tag="H181DRAFT_RS08560"
                     /old_locus_tag="H181DRAFT_01728"
     CDS             5001..6887
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="H181DRAFT_RS08560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_01728"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_158715424.1"
                     /sec_met_domain="TIGR03975 (E-value: 4.8e-190, bitscore:
                     627.7, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 5e-12, bitscore: 41.3,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGSPQPQQTLGAPARRLRVALVNMPWARSDTPSIQCGLLASVVRE
                     AGHDCAVHYLNVELAAVVGPETYTGIAEAGGERLHQFGEWLFSYAAVGDVRPEEDYHAE
                     FHEVENIWRDVTGGGRAEISALRREVLPQWLARCAEEVDWGSYDVVGFSSTFLQNTASL
                     AMGRILKERHPELHVVYGGANFDGEMGEEYLRALPWIDHVVTGEGDIAFPALLHRIAAG
                     DRAGLPGVRSRGATVPTGESPRTQDLDTLPVPDYSDYFASLRRHGETAILGDAQVKLLV
                     EFSRGCWWGQKHHCTFCGLNALGMGFRPKSGARAMEELESLLSKHPVTHVEAVDNILDM
                     KHLSTLCTDLASRHWDVEIFYEVKANLTRAHLAVLKRAGIHRIQPGIESLSTHVLDLMR
                     KGSTKLINVRLLKWARYHGITVDWNILSGFPGETDADYEEQARTVPLLHHLQPPACGGV
                     IWLERFSPYFTDPSLGLSNVRPRTCYGYIYPDTLDHSKIAYYFDYDTDAVASQGARESL
                     YSAVGEWRATWREQESPTLVYRRLPGRLEVVDRRTGQEQRTVVEGWRAEAYEACGDTAH
                     SAKRLHQDLLDAGADVALDGVTSFLDECCDTGLMVSEDGKYLALALPENPHL"
     gene            complement(7110..8651)
                     /locus_tag="H181DRAFT_RS08565"
                     /old_locus_tag="H181DRAFT_01729"
     CDS             complement(7110..8651)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /locus_tag="H181DRAFT_RS08565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_01729"
                     /product="sodium:solute symporter family protein"
                     /protein_id="WP_107068707.1"
                     /transl_table=11
                     /translation="MNKIAESSSIYLFVFLGSLVLMLALGIWVARRNRTGEDFLLAGRR
                     LTIPFVTGSALATLVGTGSSLGAVSLSYTNGWAGALYGLGGAVGIFLLLWLFADVREHG
                     FMTFPEEMSYYYGANRIIKGAVAVVLFLATVGWLGAHILGGALYLSFLTDMDMTLAKIV
                     VAVGFGLYTVIGGYWAVVVTDMVQGTILFLGFSVIAVLALVKAGGFDAVNEKAPAEATS
                     FLGVETLGTVPAISMALVIAVGVLATPSHRQRIYSANSTRTARRSFALVGVLFAAFAIT
                     PPIVGLSAQTLRPGMDNPDLAFPALATEVFPVWVGALLLVSGLSATMSSGDSEALTGVT
                     IFLRDVIKLVTGKMPKADNMVVYSRIALVAVLLFALGGALLAETIIEYITTMIATVLTG
                     LLIAALLGKFWNRATWQGGLGAIVGGAAAATAVNLSDSLTEYWGNPVIPSLAVALLVGV
                     LVSLVTPRRTISDAEALRLLAVERAELDVGTQLRTGDSTPQPAPTAPDETTPDRS"
     gene            complement(8670..10277)
                     /locus_tag="H181DRAFT_RS08570"
                     /old_locus_tag="H181DRAFT_01730"
     CDS             complement(8670..10277)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /locus_tag="H181DRAFT_RS08570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_01730"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_045863691.1"
                     /transl_table=11
                     /translation="MNRNRVLLHGGEVVDGTGAERRRADVLVENGRISAVAGISRDIDA
                     SVMDVSGSVVFPGFIDVHAHDDLALLRPDGVEPKVMQGVTVDVVGNCGHGCAPVATDPL
                     MQASYSGPVLGRFPESPPWRSFPEYLDHLSATGLRTNAVALVPHGALRASISGYARRPL
                     DDRELARATRDLDEALERGAAGLSLGLMYAPGDAADRSELLALARVVAARGRILAAHIR
                     SEGDDIAGSLRELLDIARGSGVRVQLSHLKVVSPRNKGRMPEILTLLDAARSEGIDITA
                     DVYPYTAGSTTAAALFPNWALADGVPGLLASLDAPSERAEIVASMHRPWRRQENNFRAL
                     GADGILLAGFRQPVNEHYEGARLSDVADERGEDSAECLCRLMTEERGELTVVMFQMDEA
                     DVTAALSWPWSLIGSDGLPVQSPYTHPRMYGTFARVLTEYVRERGLLTLEEATRRMTAL
                     PAERFRIPARGVIRAGAAADLVVASPERIEDHATFAAPRRFPEHVRAVLVDGEFATLDG
                     AATESTAGALLPIERSTA"
     gene            complement(10274..11518)
                     /gene="thrC"
                     /locus_tag="H181DRAFT_RS08575"
                     /old_locus_tag="H181DRAFT_01731"
     CDS             complement(10274..11518)
                     /EC_number="4.2.3.1"
                     /GO_function="GO:0004795 - threonine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="thrC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /locus_tag="H181DRAFT_RS08575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_01731"
                     /product="threonine synthase"
                     /protein_id="WP_139131682.1"
                     /sec_met_domain="PALP (E-value: 1e-71, bitscore: 236.6,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQPGSFAALGECTMCARKQDTAFPDYCVHCGGSVVLPPTGPPLP
                     DTTNLPGMWRYAGRLPITPETAWLSMGEGGSPVVPAAGTADWCGVERLLFKLDHLNPSG
                     SFKDRATAVGMAQAVRQRASAVVCASSGNAAGSTAAYAARAGLPAVVLMPEAVPPGKAA
                     VARAHGAAVLRVRGDYSNTFRAARELAGRFGWINLTTTFVNPVAVAGLKSAGYELAGQL
                     ADPPDWIVVPVGAGPLVHGIATAYRELHGSGDVAGVPRILGVQAEGCAPVAEAFARGAA
                     EVAPWQRVRTRISAISDPLRGYPSDGTYTLRLVRETGGAMATVADADADEAANQLAHRE
                     GLLIESGAAAGLAAVRTARERGTVRGDESVVCMLTGHGIKTLSTDVDPGPVITGTDEAV
                     RRLRTRTELAEGMAG"
ORIGIN
        1 ctccggcagg gcgggttccg tccgggcgtc ggccggtttg cgggcccgga tgatcgcggc
       61 gtgcaggccg tccgcggcct cctgggtgaa ctcgacttcg gcgtccttca gcccgacggc
      121 gctcagcaac tccacgtact ccgagacgga gagagcaccg gcgatgcatt cgctgtggct
      181 gccgcgttcg gcgcgctcct cggggctgag gcggtcttcg gcgacgacgt cgctgatgcc
      241 gaggcgtccg ccgggtgcca gcaccctggc catctcggcg aagacggcgg gcttgtcgac
      301 cgacaggttg atcacgcagt tggagatgat cacgtcgact gttccggccg gcagcggaac
      361 ggcctcgatg ctgcccttga ggagcaccac gttgtcggcc tctgcgtccg cgacgttcgc
      421 ggcggccagg gcgagcatct cttccaggaa gtccaggccg aagacccgcc cgtcggggcc
      481 gacgcggcgg gagctgagaa tggtgtcgag accggctccg gaccccagat cgagcacggt
      541 ctcgccttcg cgcagttcgg cgacggcggt ggggttgccg cagccgaggc tggccaggag
      601 agccgcgtcc gtcgccgccc ctggccgaac gtcctggtag gcggcgggcc cgaaggtctc
      661 cgggtcgggt gagcagcagg aaccgtccgt cccggcctcg tcggccttgg agcggtagta
      721 ggcgcgcact tcgtcctgga gcttcgcggc ggtgccggat gtgtcggtca tggacgggtc
      781 cttcggtcag gcgccggcgg gggcgaggcg gtgcacggga cggggatagc cgggtttgaa
      841 cgacaccggt gaggtggctg aggagcgggc tccggtggcc tcgtcggccg ctgccacggc
      901 gtcggtgacg gcgcggtcga ggtcgtccgg ttcgcacgcg acgcgggcgt tgacgaccgc
      961 gcgggcgtcg gccacggcgg ttgcgaggac gctgtccacg gtgggcgtcg cacccgcttc
     1021 ggtgagactc agcttggcga actcgccggt gccgtcgtcc acggtgagct tcgcgtgtcc
     1081 gatcaccgca ccgtgctccg ctgcccagcc ggagagacgg gcgaggaccg tacgggccca
     1141 gtccgtcgcg tcgaactcct cgccgtcggc ggacagttcg acttcctggt tcatccaggc
     1201 cagctgggcc tcggccgcgg cgtaccggtc gtagtcgacc tccaggtcca ccgcctggtt
     1261 tcccgcgggt gcctcccagg cagccaggag tgtgtccacg ccctcgcctg tggtcgccga
     1321 gtagcccacg acggcggcgt cgccgtactg cttccgcagt ccgggcagca gctcgtcggc
     1381 ctggtcgccg gtgatcgtgt cgagcttgtt cacggcgatc acgtcagcct cggtgagctg
     1441 ctgccggaag aggtaggaca ggtccgactc cggctcaccg cgctgcgccg cccgctcgaa
     1501 ggcccggtgg cgcagcgggt cgacgacggt cgtcaggggt gcgacgacca tgttgtcgcc
     1561 gtagtagcgg cgcagcggcc gtaccacggt ggcctgcagg tcggtgcagc tgccgacggc
     1621 ctcggcgatg accgtgtcga cgccgtcgga gccggtcagg gcgaccaccg cctcgacgag
     1681 atcctcgaac ttgcagcaga agcacccgcc ggtgacctcc gcgacgctgt ccagcttgct
     1741 gcgtacgagc tgggtgtcca cgagctccac accctggtcg ttggtgatga cggcgaccct
     1801 gtggcccttc tcctgcaggg ccagggcggt cgtggtcatg gtcgtggttt tgcccgcgcc
     1861 caggaacccg gtcagcggaa tgaaggtgat catgtgattc cccgtctcgt gggtcggagg
     1921 tcggatcaac cggcggagca gcagccgccg gacgattcgc tcagcagcgg cggcgcggcg
     1981 ggacgggacg gcaccgcgtc cccgaacgcg ctgtgcctgc tcgatccgaa cgcgtcgagc
     2041 caagcggccg cccggggacg caggttcgga tcggagagca gcagcgacgt ggtcatggcg
     2101 cggggcgtgc agccgcgcag aaccttcgcc cggtgagcgc cggcaccgaa ctcgacggtc
     2161 cgcagccctt cggcgtaggc gtcgcgcacg ggggcgtcga ggacgagggc gaagtagacg
     2221 ccgctgcgtt cgccgatggc cgcgtagtcg aagcccgccc acttggggaa gagccggtgg
     2281 tgcttgcgta cggtgacgca gctggcgacc agctcaccgc tcttgcgtcc gaggtaggcc
     2341 cgtacgtccg ctccctcgtc gatgagtgcg gagaggatgc cggcgatgtg gccgggctcc
     2401 tcgccggcgc cgttcttctc acggttgagg caggtgagct ccgcgatgcg ctcgacgtag
     2461 gggcgtatgc cggtgccgtc ggcgcgctcg atggtgatgc cggcggccgc ggccttgcgg
     2521 acgtccgcct tgatgcgctg acgcttcttc agggggaggg cggcgaggtg gccttcccag
     2581 tccggggcgt cgaggctcag gaagtcctcg tacccccaga agcagtggtg tccgcctgcc
     2641 gagtcgaggg cggtcaccag gtcccggccg acgcgccggt cggggatcca gggcgcgagg
     2701 acgcagcgga cgccggcgtc gaacgcggcg ggaaccagct tctccacgag ggtccccaca
     2761 agggtgggcg tccagaagtt gtacgcgggc tcggtgcggt agccgagcgg ggaacccagc
     2821 agcagggtgg ggaagaacgg ctcggtgccc agttcgtcga tctcggcgct gcgtgcggag
     2881 tccgtctccg ctccgcagca gacctcctca cccgtgggag cctgccggcc caggtaggtg
     2941 cggggttcgt ggtcgaccac cgggggtgag gcgaggacgt atccgggcag gacggccagc
     3001 tccccgcggg cgcgggtggc gacggtgtgc cacggctggg cttccgggag ggccttctcg
     3061 gtggccgcca gccaggtggg gcccagatac ggccaggcat cggtgaggtc ggtgagctcc
     3121 tcggaccagc ccatccgcgg aagcgcctgg ccggtggaga cgacgacgcg aggtgatccg
     3181 cccatctcgg gctccttcgg gtgggaggga ccggtcagca cttcacgcag caacggaccc
     3241 tgcccgcgtc ctccgcgtcg gatcttcgtt gctgccacag ctcctcgacc cgcctgtcgg
     3301 ggtgcgtcca ctccgtccag aagccgcggt cgacggtcgc gacgtcctcc atccacggtg
     3361 aggacagcag cggcgagcct ttgaacagca gcagccgggt ctggagggcg gtggggtcgg
     3421 tgacgtgggc gaggtctgcc tccaccagcc agtcctcgaa gccgagcagt tcttcgtact
     3481 cgacccaggg ggtgaagggg atgaacgtcg gatacagctc gaagccgatg cgtctggcct
     3541 cgacgaccgc ctggcgcatg tggtcgacgt cgatgtgctt gtcgaagatg cgcaggacgc
     3601 ggtcggaggg gaactccagg gcgcaggtga cgcggcgcag tccgagggag accagcttct
     3661 ccagctcacg tggatagctc aggacgtggt cgacccgtgt ggtgaactcg aacgtgatcg
     3721 ggccgacggc gtccgcgagc cgttccacga ttccggcgcc gatcgtgcgg gagttgaaga
     3781 actccgcgtc gatgaagcag aagtggcgta caccctcgtc cgcgagctgg agggcgtcgg
     3841 ccaggacgct gtcggcttcg tagacggcga caccgccgtc gtaggcgccg tagacggaac
     3901 agtaggtgca cttgtggtgg cagccgcggg acgcctcgat gttccccatc agcccgaacg
     3961 gcgagtggtg ggcggggtag tggacgaggg aggggaagag atcacgggcg ggcgcggcca
     4021 cggagatccg gcggcggggc ggcctggggc gcatcccccg ggacgtcatc agggcaggga
     4081 ccgcgtgcag gtcgcgcgtg ccgcgcgcca ggtcggacag ttcctcgccg atgagctcgg
     4141 gttcctccat gacgacggcg tccgccgcct tgaggaactc gcggtggttc atctgcgcgt
     4201 actggccgaa cgccacgacg gtggtggcga accgctcctt gagctcacgt gccaggctca
     4261 ggccgcgctc cagcccctcg aactgctgga ccgacaccag ggccaggtcg aacgggcctt
     4321 cagggaaggc atcgggctgg aagtgcgcgt cccagccgac ggtctccacg tcgtgagcct
     4381 gcagggaagc tgccgcgtac gcacaggtca gaggctgcat ctccgcttcg aaagcggagg
     4441 cgacgagcac acgtggctga gtcacacttc cccccgggag tgagtcaatc caagaaagcg
     4501 aaccgtgcta aacgcgctgt gccctgtcgg tgagcgaagg ctcgggacgg ccggaacatt
     4561 gccgcctcta gcgatacatg tcaatagacc gatacggagc cgaccgcgac gaccctgttg
     4621 acgacaggtt cggtgcgccg ccgccagggc ccgcacggcg tcccggggcg gtgcctgaat
     4681 ctcgtcatgt gccgacttca gaagtcgaat tgagctcgct tgccctccgc ctgggtggaa
     4741 gtgtgatccc cgggggccgg attcctcgca gtcggtggtt cgcgtgtggc ccgtgaggct
     4801 ccatcggcat tcttcgatcg tgatcccgtg ccgagggctt cccacacgac ggaggcgtcc
     4861 ctacactcct gacggactct ccaagccaga cccagttcga accacggctc cactcggcgt
     4921 ccgcaccgcg tgtccgcgac gccggtacgg acagatccgg acccgagagg actggcatgt
     4981 cgccgaagac gctccccctg gtgggatcac cccagccaca gcagacgctc ggtgctccgg
     5041 cgcggcgact gcgggtcgca ctcgtcaaca tgccgtgggc gcgctccgac acgccgtcca
     5101 tccagtgcgg gcttctcgcg tccgtcgtgc gggaggccgg tcacgactgc gctgtgcact
     5161 acctcaacgt cgaactggcg gccgtcgtgg gtccggagac ctatacgggc atcgccgagg
     5221 cggggggtga gcgtctgcac cagttcggcg agtggctctt ctcctacgcc gctgtcgggg
     5281 acgtccggcc cgaggaggac tatcacgccg agttccacga ggtggagaac atctggcggg
     5341 acgtcaccgg aggtggccgc gccgagatca gcgctctgcg ccgtgaagtc ctgccccagt
     5401 ggctggcccg ctgcgcggag gaggtcgact gggggtccta cgacgtcgtc ggcttctcct
     5461 cgaccttcct gcagaacacc gcttcactcg ccatgggccg catcctgaag gaacgccatc
     5521 ccgagctgca cgtcgtctac ggcggggcca atttcgacgg ggagatgggc gaggagtacc
     5581 tcagggccct cccctggatc gaccacgtcg tgacgggaga aggggacatc gccttcccgg
     5641 cgcttctgca ccgcatcgcc gccggcgacc gcgcaggtct tcccggagtg cgatccagag
     5701 gtgcgaccgt cccgaccggg gagtccccgc gcacgcagga cctcgacacc ctccccgttc
     5761 cggactactc cgactacttc gcgagcctgc gacggcacgg cgaaacggca atcctgggcg
     5821 acgcgcaggt caagctgctc gtcgagttct cccgcggctg ctggtggggg cagaagcacc
     5881 actgcacctt ctgcggcctc aacgcactcg gcatgggctt ccgccccaag tcgggggccc
     5941 gggccatgga ggaactcgaa agcctgctga gcaagcatcc cgtcacccac gtcgaagccg
     6001 tggacaacat cctcgacatg aagcacctgt cgacgctgtg cacggacctc gcgtcgcggc
     6061 actgggacgt cgagatcttc tacgaggtca aggcgaacct gacacgggcc catctcgccg
     6121 tcctcaagcg tgcgggaatc caccgcatcc agcccggcat cgagagcctg agcacccacg
     6181 tcctcgacct gatgcggaag ggctccacca agctgatcaa cgtccgtctg ctcaagtggg
     6241 cgcgctatca cggcatcaca gtcgactgga acatcctcag cggcttcccc ggggagaccg
     6301 acgccgacta cgaggagcag gcccggacgg tgccgctgct gcaccatctt cagcccccgg
     6361 cctgcggcgg ggtgatctgg ctggaacgct tcagcccgta cttcacggac ccgtccctcg
     6421 gcctgtcgaa cgtgcgcccc cgcacctgct acggctacat ctatccggac accctcgacc
     6481 actccaagat cgcctactac ttcgactacg acacggatgc cgtggcctcc cagggagccc
     6541 gcgagtcgct ctactccgca gtgggcgaat ggcgcgcgac gtggcgggag caggagtcgc
     6601 cgactctcgt ctaccgccgc ctccccggtc ggctcgaagt cgtcgacagg cgcaccggcc
     6661 aggagcagcg caccgtggtc gagggctgga gggccgaagc gtacgaggca tgtggcgaca
     6721 cagcgcacag cgccaagcgt ctccaccagg accttctcga cgctggcgcc gacgtggcac
     6781 tggacggggt gacgtcgttc ctcgacgagt gctgtgacac cggtctgatg gtcagcgaag
     6841 acgggaagta cctcgcgctc gcgctaccgg agaatcccca cctgtagagg agcaatgcac
     6901 cgcgttcagc tcgtacggac tctcgccgtg ctattcgaat agcagcgggc aggcatgctc
     6961 cgtgaccgcc acctcgcagc cgatcctcct caattgctcg ttcttgtcga cggttatcgg
     7021 gaggggtggc tgccttacgg cgaggtagcc ctgagcagga gcgttccgac cacgcctccg
     7081 ctcccgcgcc ggtacgtccc cgagggctgt cacgagcggt caggggtggt ctcgtcgggg
     7141 gcggtggggg cgggctgcgg agtgctgtcc ccggtgcgca actgcgttcc cacatcgagc
     7201 tccgcccgtt cgacggcgag cagtcgcagc gcttccgcat cgctgatcgt ccggcgcgga
     7261 gtcaccaggc tgaccagtac gcccacgagc agggccacgg ccagtgacgg gatcaccggg
     7321 ttgccccagt actcggtgag ggagtcgctg aggttgacgg cggtcgccgc ggccgcgcct
     7381 ccgacgatcg cgcccaggcc gccctgccat gtggcgcggt tccagaactt gccgaggagc
     7441 gcggcgatca gcagaccggt gagcactgtc gcgatcatcg tcgtgatgta ttcgatgatc
     7501 gtctccgcca gcagggcgcc tccgagtgcg aacagcagca ccgcgaccag ggctatccgt
     7561 gagtagacga ccatgttgtc cgccttcggc atcttccccg tcaccagctt gatgacgtcg
     7621 cgcaggaaga tcgtcacgcc ggtgagcgct tcggagtccc ccgacgacat ggtcgccgag
     7681 aggccggaga cgagcagcag cgcgccgacc cacacgggga agacctcggt ggcgagggcg
     7741 gggaacgcca ggtcgggatt gtccatgccc gggcgcaggg tctgggcgga gagccccacg
     7801 atgggcgggg tgatggcgaa ggccgcgaac agcactccga ccagtgcgaa gctgcggcgc
     7861 gctgtccgcg tgctgttcgc cgagtagatc cgctgcctgt gggagggcgt ggcgagtacg
     7921 cctaccgcga tcaccagggc catcgagatc gccggtacgg tgccgagcgt ctcgacgccg
     7981 aggaaggacg tcgcctcggc aggcgccttc tcgttgacgg catcgaagcc accggccttg
     8041 acgagcgcca ggacggctat cacggagaat ccgaggaaca ggatcgtgcc ctgcaccatg
     8101 tcggtgacca cgacggccca gtagccgccg atcaccgtgt acaggccgaa accgaccgcg
     8161 acgacgatct tggcgagagt catgtccata tcggtgagga acgacaagta cagcgccccg
     8221 ccgagaatat gggccccgag ccagccgacc gtggcgagga acaggaccac tgcgaccgcg
     8281 cctttgatta tccggttcgc cccgtagtag tagctcatct cctccgggaa cgtcatgaag
     8341 ccgtgctcgc ggacgtcggc gaacagccag agcagcaaga agatccctac cgcgccgccc
     8401 agcccgtaca gcgctcccgc ccagccgttg gtgtacgaca ggctgaccgc accgaggctg
     8461 gacccggtgc cgacgagcgt ggcgagcgcg ctgcccgtga cgaagggtat ggtcagccgc
     8521 cgtccggcaa ggaggaagtc ctcaccggtg cggttgcggc gggcgaccca gatgcccagg
     8581 gcgagcatga gcaccagcga gccgaggaag acgaacaggt agatggagct ggattcggcg
     8641 atcttgttca tgggtgaacc ttctgttcct caggcggtac ttctctcgat cggcagcaga
     8701 gcacccgccg tgctttcggt agcggccccg tcgagcgtcg cgaactcgcc gtcgaccagt
     8761 acggcgcgga cgtgttccgg gaagcggcgc ggagctgcga atgtcgcgtg atcctcgatg
     8821 cgttcgggac tggcgacgac caggtcggcg gcggcacccg ctcggatgac gcctcttgcg
     8881 ggaatccgga agcgctccgc gggcagagcg gtcattcgcc gggtcgcttc ctcaagagtg
     8941 agcaacccgc gctcgcggac gtattcggtg agcacccggg cgaaggtgcc gtacatccgt
     9001 ggatgcgtgt acggcgactg gacgggcagg ccgtccgatc cgatgaggga ccacggccag
     9061 gacagtgcgg ccgtcacatc ggcttcgtcc atctggaaca tgacgacggt gagttcgccg
     9121 cgctcctcgg tcatgagccg gcacaggcac tccgcggagt cctcgccgcg ttcgtccgcc
     9181 acatcggaca accgggcacc ctcgtagtgt tcgttgacgg gctgccggaa gccggccagc
     9241 aggatgccgt ccgcgccgag tgcccggaag ttgttctcct gacggcgcca cggccggtgc
     9301 atgctcgcca cgatctcggc gcgttccgac ggggcgtcca gcgaggcgag cagtcccgga
     9361 acaccgtctg cgagtgccca gttggggaac agcgccgcgg cggtcgtact gcctgccgtg
     9421 tacggataga cgtcggcggt gatgtcgata ccttccgacc gcgcggcgtc gagcagggtc
     9481 aggatctccg gcatgcgccc cttgttgcgc ggcgagacca ccttgaggtg actcagctgc
     9541 acccttaccc cgctgccccg tgcgatgtcg agcaactccc gcagggagcc ggcgatgtcg
     9601 tccccttcgc tgcggatgtg tgccgcgagg atccgtccgc gggcggcgac gactcgcgcc
     9661 agcgccagca gttcggaacg gtcggccgcg tcgccgggtg cgtacatcaa accgagtgac
     9721 aggccggccg caccccgttc cagggcttcg tccagatcac gtgtggcgcg ggcgagttcg
     9781 cggtcgtcca gcggtctgcg ggcgtagccg gagatcgagg cgcgcagcgc tccgtgcggt
     9841 acgagagcca cggcgttcgt gcgcagcccg gtcgcgctca ggtggtccag atactccggg
     9901 aagctccgcc agggaggcga ctcggggaag cggccgagaa ccggcccgga ataggacgcc
     9961 tgcatcagcg gatcggtggc caccggcgcg caaccgtgcc cgcagttgcc gaccacgtcc
    10021 acggtcacgc cctgcatgac cttcggttcg accccgtccg gccgcagcag cgccaagtcg
    10081 tcatgtgcgt gcacgtcgat gaagccggga aagacgaccg atcccgacac gtccatgacg
    10141 gaggcgtcga tgtcgcgcga gatgccggcg acggcgctga tccggccgtt ctccaccagc
    10201 acgtcggcgc ggcggcgttc ggcacctgtt ccgtcgacga cctcgccgcc gtgcagcagc
    10261 acccggttcc ggttcacccc gccattcctt ccgccagttc ggtgcgcgtc cggagccggc
    10321 gcaccgcctc gtcggtaccg gtgatcacgg ggcccgggtc gacgtcggtg gacaacgtct
    10381 tgatgccgtg accggtgagc atgcagacga cgctctcgtc cccgcggacc gtgccgcgct
    10441 cgcgcgcggt ccggaccgct gccaggccgg ctgcggcgcc ggattcgatg agcagccctt
    10501 cccggtgagc gagttggttc gcggcttcgt cggcgtcggc gtcggcgacc gtcgccatgg
    10561 ctccgcccgt ttcgcgcacc agccgcagtg tgtaggtgcc gtcggacgga tagccgcgca
    10621 gcgggtcgga tatcgccgag atccgggtcc gcacgcgctg ccaaggggcg acttcggccg
    10681 caccgcgggc gaaagcctcc gcgaccggcg cacagccctc ggcctgaacc ccgaggatcc
    10741 gcggtacccc cgcgacgtcc cccgagccgt gcagctcccg gtaggcggtc gcgatgccgt
    10801 gcacgagcgg gcccgcgccc accggcacca cgatccagtc cggcggatcg gccagttgac
    10861 ctgccagttc gtatccggcg ctcttcaggc ctgcgacggc gaccgggttg acgaaggtcg
    10921 tggtgaggtt gatccacccg aagcggccgg ccagttcccg tgccgcgcgg aaggtgttgc
    10981 tgtagtcccc gcggacccgc agcaccgccg cgccgtgcgc gcgggcgaca gcggcctttc
    11041 ccgggggcac cgcctccggc atcagcacca ctgccggcag gcccgccctg gccgcgtacg
    11101 cggctgtcga acccgccgcg ttgccactcg aggcacacac gacggcgctg gctcgctgcc
    11161 gcacggcctg cgccattccc acagcggtcg ccctgtcctt gaaggaaccc gacggattga
    11221 gatggtcgag cttgaacagc agacgctcga caccgcacca gtccgcggta ccggctgccg
    11281 ggacgacggg gctgccgccc tcccccatgc tcagccacgc ggtctccggt gtgatcggca
    11341 accggcccgc gtagcgccac atgcccggca ggttcgtggt gtccggcaag ggcggcccgg
    11401 tgggagggag caccaccgag ccgccgcagt gcacgcagta gtccgggaac gccgtgtcct
    11461 gcttgcgggc gcacatggtg cattcaccga gagctgcgaa cgatccgggc tgtgccacgt
    11521 cattcctcca cgccgtctca cgcactcgct gaggggcggt accgaaccaa cgatcacttg
    11581 catattttgc aagtcccttg cgtataatga cgcgaagcta gttcgggtgt ctcgtgccgt
    11641 caagggatcg gtccgtgccg cccagccgga gcgaggagta cgggtgtatg agtgagagcc
    11701 tccggcgcgg gctgagagtc ctcgccgcac tgtccgagga accggctacg gcgagccggg
    11761 tcgcgcagtc gcacggcgtc agcctctcca cagccgtgcg gctgctccag ctcctggccg
    11821 acgagggttt cgcccggcgc gacgagacgg gcaggtacca cgtcgggagc cagctgttgc
    11881 aggtcgc
//
