LOCUS       NZ_FMHJ01000001        10984 bp    DNA     linear   CON 28-JUL-2024
DEFINITION  Streptomyces sp. WMMB 714, whole genome shotgun sequence.
ACCESSION   NZ_FMHJ01000001
VERSION     NZ_FMHJ01000001.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces sp. WMMB 714
  ORGANISM  Streptomyces sp. WMMB 714
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Varghese,Neha. and Submissions,Spin.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2016) DOE - JOINT GENOME INSTITUTE, 2800 Mitchell
            Drive, Walnut Creek, CA 94598, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900090125.1-RS_2024_07_28
            Annotation Date                   :: 07/28/2024 02:17:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,656
            CDSs (total)                      :: 5,578
            Genes (coding)                    :: 5,496
            CDSs (with protein)               :: 5,496
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 6 (5S)
            partial rRNAs                     :: 5, 4 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 82
            CDSs (without protein)            :: 82
            Pseudo Genes (ambiguous residues) :: 0 of 82
            Pseudo Genes (frameshifted)       :: 15 of 82
            Pseudo Genes (incomplete)         :: 72 of 82
            Pseudo Genes (internal stop)      :: 4 of 82
            Pseudo Genes (multiple problems)  :: 8 of 82
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 06:07:12
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 161809
            Orig. end                         :: 172793
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FMHJ01000001.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10984
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10984
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10984
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5984](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5984
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    152..166
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.1"
                     /tool="antismash"
     gene            complement(492..977)
                     /locus_tag="H181DRAFT_RS00510"
                     /old_locus_tag="H181DRAFT_00106"
     CDS             complement(492..977)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H181DRAFT_RS00510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H181DRAFT_00106"
                     /product="hypothetical protein"
                     /protein_id="WP_045862142.1"
                     /transl_table=11
                     /translation="MLAALRAGAALDAPAGALGVPVEHLAAFVLRDSEVRSALAGHSPE
                     EQLRARRHDFLTALRGTDGDRELAAWAVVLDVLDTVEWLADPVYAAEEALLLTAVAERA
                     SRPRRRIADELLDRAAELLETGATITEAARRVGVATGTLRSRSGGHPRLAAALPPKR"
     gene            complement(1302..2120)
                     /locus_tag="H181DRAFT_RS00515"
                     /old_locus_tag="H181DRAFT_00107"
     CDS             complement(1302..2120)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /locus_tag="H181DRAFT_RS00515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_00107"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_052708387.1"
                     /transl_table=11
                     /translation="MPRLDGPPHRRHDLQNFLREARARVDPAEHGLERPSDRRGRQVPG
                     LTQPQMDVLLHSRTGTYGSFERGAKTNPSTDYLDRLARYLKLNPQEWESLWRHTYGHNP
                     PYALDPGAGLDVAPVWRRVVHEMTTMAFISNVCWDLLECNSAFAEMFPDGKPPENVMRW
                     MLLDDGARDEVLLDWENEWAPVIIPQLVAARHAYPADPTLQELEKDLHSDPRAARVRRR
                     IAAAFTRPDSDDRPLRHPVLGPGRARLGAAEPLVNPGARLMVVEFEPDRP"
     misc_feature    2163..2176
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.09"
                     /tool="antismash"
     misc_feature    2318..2334
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.42"
                     /tool="antismash"
     gene            complement(2458..3075)
                     /locus_tag="H181DRAFT_RS00520"
                     /old_locus_tag="H181DRAFT_00108"
     CDS             complement(2458..3075)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="H181DRAFT_RS00520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_00108"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_158715404.1"
                     /transl_table=11
                     /translation="MAAARSFDRSGYQRTTLFEVSRAAAMSKGALSFHFGSKEELADTV
                     RREACTRTFQATELLRAKAQPALQTLIDLTHLVAEQLANDHLARAAVKLSRELETPPDP
                     EVNPLACWRQVFTEVAREAEKDGTLRPETTAETVAEVAFGLASAAPTDQAEGGADPHER
                     LSAHWQLLLYGLVSQPGPEHLWIAGTESDWLRVSGCELQKAE"
     misc_feature    complement(3205..3225)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.62"
                     /tool="antismash"
     gene            complement(3307..4269)
                     /locus_tag="H181DRAFT_RS00525"
                     /old_locus_tag="H181DRAFT_00109"
     CDS             complement(3307..4269)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: TD (0-134). E-value: 5.9e-08. Score:
                     24.2. Matches aSDomain:
                     nrpspksdomains_H181DRAFT_RS00525_TD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742094.1"
                     /locus_tag="H181DRAFT_RS00525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_00109"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_254666653.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 4.2e-12, bitscore:
                     40.8, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSGATGFLGSAVLARLGAHGAPGAPRTRERPGTAQELTVRAFTR
                     RHVALPAGVEQVHADLARPETLAGVCEGADVLVNAASHVGSDEQQCNLVNDLGTAALME
                     EARRAGVRRVVHLSTTAVYGRGPHRGASVTDLGPAPGSAASRSRLAGETHALRQGALVL
                     RAGLVTGEGDRWVVPALAELARRVPGLWLGGTGRLSLVAVEDLARLVTAAALRLPAAGG
                     AVHHAVHPQPVRLRDLLRTLAALGLLPPLSGELTWQQCLSLMRANPGRIRERQLELLAQ
                     DHFYSGESLWDACRCDPGPGPLARLPGAAAWYRRQLGLV"
     aSDomain        complement(3868..4269)
                     /aSDomain="TD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_H181DRAFT_RS00525_TD.1"
                     /evalue="5.90E-08"
                     /label="H181DRAFT_RS00525_TD.1"
                     /locus_tag="H181DRAFT_RS00525"
                     /protein_end="134"
                     /protein_start="0"
                     /score="24.2"
                     /tool="antismash"
                     /translation="MLSGATGFLGSAVLARLGAHGAPGAPRTRERPGTAQELTVRAFTR
                     RHVALPAGVEQVHADLARPETLAGVCEGADVLVNAASHVGSDEQQCNLVNDLGTAALME
                     EARRAGVRRVVHLSTTAVYGRGPHRGASVT"
     gene            complement(4281..4898)
                     /locus_tag="H181DRAFT_RS00530"
                     /old_locus_tag="H181DRAFT_00110"
     CDS             complement(4281..4898)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986960.1"
                     /locus_tag="H181DRAFT_RS00530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_00110"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_045862144.1"
                     /transl_table=11
                     /translation="MQQRAVETRQLILEKAAEVFDEGGYRAARIADIMHRAGITQGALY
                     FHFSSKLDLARTVMLAQRDAVVLPPGPDGLQRLIDTTLLLAFELQTNKLLRAGVRLAVE
                     QEEIGFVDDAPYREWAQVFRGQLEAAAKRGELRSHVEVDRLAVLLVGCYTGVQHFSKLA
                     NARADLPDRIADLWHYMLLGIAPPQVAAEMVVNPEHPMLAAG"
     misc_feature    4855..4868
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.11"
                     /tool="antismash"
     gene            5001..5984
                     /locus_tag="H181DRAFT_RS00535"
                     /old_locus_tag="H181DRAFT_00111"
     CDS             5001..5984
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="H181DRAFT_RS00535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_00111"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_052708389.1"
                     /sec_met_domain="AfsA (E-value: 3.7e-19, bitscore: 63.7,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVTPAVPDLPESTAYSSPVPREFVHKSAHTEVLLTGWRRLGERE
                     FEVGAQWPRDHGYWRTGRGGVQDPMLLVETTRQVLPLLSHAVYRVPPGHQLIWSHHHCA
                     FAAPLLRVTDRPAEVVLRLSVSRPPLPGGRLRRLAVTMTAACEGRTLGTASTVFSAHSP
                     EVYARLREPRTDPAEAMSAALPPPAPVVPALVARESEREVVLAPAEDAARRRLRVVTSS
                     PWLFDHPVDHVPGMLLMEAARQCAHLVLQGRRSRLLHLVRMEAEFKRYVDLDEPAWVDW
                     SQPSSRPDSPSEDGEETMTVAITQRGVRAFTAVVGFAAAAVLPGAG"
     gene            6191..7030
                     /locus_tag="H181DRAFT_RS00540"
                     /old_locus_tag="H181DRAFT_00112"
     CDS             6191..7030
                     /GO_function="GO:0008276 - protein methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518540.1"
                     /locus_tag="H181DRAFT_RS00540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_00112"
                     /product="putative protein N(5)-glutamine
                     methyltransferase"
                     /protein_id="WP_045862145.1"
                     /transl_table=11
                     /translation="MTSPLPSPLPRSALVARLRAAGCVFAEDEADLLISTAGSPHELAT
                     MAGRRTAGEPLEHVLGWAGFCGMRTAVEAGVFVPRRRTEFLVRQAAEALRAVAGRCREE
                     AAAPVLVDLCCGSGAVGAALASQADGRVELHAGDVDPAAVRCARHNLAAYGGHVHHGDL
                     YEPLPAGLRGRVDVLVANVPYVPADEVPLLPPEARLHEPRVALDGGPDGLDVLRRVAAG
                     APGWLAPGGSLLVETSERQAPRALEAVADCGLVARAVTCEEFDATVITAARPDAGRA"
     gene            complement(7161..8657)
                     /locus_tag="H181DRAFT_RS00545"
                     /old_locus_tag="H181DRAFT_00113"
     CDS             complement(7161..8657)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546261.1"
                     /locus_tag="H181DRAFT_RS00545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="H181DRAFT_00113"
                     /product="cytosine permease"
                     /protein_id="WP_078895437.1"
                     /transl_table=11
                     /translation="MTVEANGLNVIEDAERKGRPAQLFWPWFGSNVSVLGLSYGSFALG
                     FGIDFWQALAAGVAGIVFSFLLCGFIAVAGKRGSAPTMVLSRAAFGVRGNRLPSAVSWV
                     LTVGWETVLVILATLATSTVFDRLGWGGGTSTKIAALVVVSALTVLTGVVGFDLIMRMQ
                     SAITVVTAALTVVYIALVAGEIRWETAGSLPAGSAQEVVGALVFLMTGFGLGWVNAAAD
                     YARYLPRRTRGAAVAGWTTFGASLAPLVLLVFGLLLAGSSPRLSEAIAADPVGALTTLL
                     PTWFLVPFALVAVLGLVGGAVLDIYSSGLALLALGVRVPRYAAAALDGLLMILGTVYVV
                     FFSEDFLGSFMGFLTTLGVPVAAWCGIMLADVALRSRPYDDADLFSPSGRYGDVRWLPA
                     AVLCAATAAGWGLVTNTAAGWLSWQGYLLGPLGLGGTSGAWASANLGVLAALVSAFAVT
                     LVCHRARVKEQEAAPPSPSGPHGNARTAEDGAPGSAPTSA"
     gene            complement(8851..9420)
                     /locus_tag="H181DRAFT_RS00550"
                     /old_locus_tag="H181DRAFT_00114"
     CDS             complement(8851..9420)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="H181DRAFT_RS00550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="H181DRAFT_00114"
                     /product="hypothetical protein"
                     /protein_id="WP_045862147.1"
                     /transl_table=11
                     /translation="MPAADPVAATATRAAAPAPVRTRDVLVLGTAEVAYCVTDALRQAG
                     LSVGSWLAPADACHYRARDHIRRDLADPATLRSWSSAAVVVSVWHGSARHSHPGEVVEP
                     LVDAALARSAPHWLRLTRGVTDGGPQEAQAAVDHDPYRSTCYLPPEARTHLTRLRDQAD
                     PDGVYARTLTALLSVLPLTPGCSPRF"
ORIGIN
        1 gccgacggtc acgaagaagt aggcgaggta cagctgcacc gcccacaggg ccacttcgag
       61 ggcgatccgg gcggggctct tgcggcgtga acgcctggct gaggggttgg cgtgagaggt
      121 cggcggcgtg ccgctcgcgt gcgccatggt ggtctccttc tgcagacggt gcgcatgcac
      181 ttttcatgac ggacccggcg gccggccttc tgccgccaga gacccgcggc ggatccccgg
      241 ccgcagcggc agcgaggcgg cgacgccctg cgaacacgtg tgccgtgggt tccaccatgt
      301 gcgtatcacg tgcgcagcgc gcaccgcaga cagttgcgca tcctgttcaa cgaacacatt
      361 gcgtacgggc ccgccgcgac ctggtccttc ggacggacgg cggcgcaggc acgcggccac
      421 cggcgtcctg ggcgggcatc atcgtgccgc cctgcggcgc ccggttcccg ggtgctgcgg
      481 gccgcgggtg ctcagcgttt cggcgggagg gcggccgcca ggcggggatg ccccccgctg
      541 cggctgcgca gcgtccccgt ggcgacgccc actctgcgtg ccgcctcggt gatggtggcg
      601 cctgtctcga gcaactcggc tgctctgtcc agcagttcgt ccgcgatccg gcgccggggc
      661 cggctcgccc gctcggcgac cgcggtcagc agcagcgcct cttcggccgc gtacaccgga
      721 tcggccagcc actcgacggt gtccagcacg tccaggacga cggcccaggc ggcgagttcc
      781 ctgtctccgt cggtccccct cagcgccgtc aggaagtcgt gccgcctggc ccggagttgc
      841 tcttcgggcg agtgccccgc gagagcggac cggacctcgc tgtcacggag gacgaacgcg
      901 gcgaggtgct cgacgggaac tcccagcgcc ccggccggcg cgtcgagggc ggcgcctgcg
      961 cggagcgccg ccagcacggc ctcacgcctc tccggaccga ggcgggtctc acgctccagc
     1021 tccgcccagc gcgcggcgcg gcacctgggg cagcgtccgc cccggtgcca tcccccgggc
     1081 tcaccggggc gcaggcccct cggctcaccg cagctggctc cagccgcctt gcaccgtgta
     1141 cgcgttctca caggggcccc gctggaccac ggtccgggcc ggccggggcc gcgaacctcc
     1201 cccttctcct catggcggac atgctaccca ccgccgccac catcaagcgg acgctctcgc
     1261 tccggcggcc cagggcggcc cgggtccggc ctgcgggcag gtcaggggcg gtccggctcg
     1321 aactcgacga ccatgagccg cgccccgggg ttgacgagcg gctcggccgc accgagccgt
     1381 gccctgcccg gccccagaac ggggtggcgc agcgggcggt cgtcgctgtc gggccgggtg
     1441 aaggcagcgg cgatcctgcg ccggacgcgt gcggcgcgcg ggtcgctgtg caggtccttc
     1501 tccagttcct gcagcgtcgg gtcggccggg taggcgtgcc gggcggccac gagctgcggg
     1561 atgatcaccg gcgcccactc gttctcccag tccaggagga cctcgtcgcg cgccccgtcg
     1621 tccagcagca tccaccgcat gacgttctcg ggcggtttcc cgtccgggaa catctccgcg
     1681 aaggccgagt tgcattccag caggtcccag cagacgttgc ttatgaaggc catcgtcgtc
     1741 atctcgtgga cgacgcggcg ccacacgggc gcgacgtcca gaccggcgcc ggggtccagc
     1801 gcgtaaggag ggttgtgccc gtaggtgtgc cgccacagcg actcccactc ctgcgggttc
     1861 agcttcagat agcgcgccag acggtcgagg tagtcggtcg acgggttcgt cttcgcgccg
     1921 cgttcgaagc tgccgtaggt gcccgtccgg ctgtgcagga gcacgtccat ctgcggctgg
     1981 gtgaggccgg ggacctggcg tccccggcgg tcgctcggcc gctccagccc gtgctcggcg
     2041 ggatcgaccc gtgctcgagc ctccctgagg aagttctgca agtcgtgccg gcggtgcggc
     2101 gggccgtcga gccggggcat tctgacgctc ctcggactgt gcgggcgttt ttgagtaccc
     2161 cgttcttaca cgtaaaaggt cgcgcctgga aaggtgccag ccgcgcacgg catgatcggg
     2221 gggcaccacg ctgccgtcgg gggacagcgt ggcgcgagac gtgatgcatg gcgggactcc
     2281 tcggctcatc agggcgacga ggagtcaggg gaacgcctgc acgtgaggtc ggcaggtctc
     2341 ccatccgccg cgggcggcct gcccggctga cgcgtcttct ccggggtccc ggaccacggc
     2401 cgaccggagc cggtcccgtc ccccgccgga cgtcgcctcc gcccgactcg ggccggctca
     2461 ctcggccttc tgcagctcgc acccggagac gcgcagccag tccgactcgg tgcccgcgat
     2521 ccacagatgc tcggggccgg gctgggagac cagtccgtac agcagcaact gccagtgcgc
     2581 cgagaggcgt tcatgcggat cggctccgcc ttccgcctgg tccgtagggg ccgccgaggc
     2641 gaggccgaag gccacttccg ccaccgtctc cgccgtcgtc tcggggcgca gcgtcccgtc
     2701 cttctcggcc tcccgggcca cttcggtgaa gacctgccgc cagcaggcga gcgggttgac
     2761 ctcggggtcc ggcggtgtct ccagctcgcg ggacaacttc accgcggccc gggccagatg
     2821 gtcgttggcc agctgctcgg ccacgaggtg cgtgaggtcg atgagcgtct gcagcgccgg
     2881 ctgggccttg gcccgcagca gctccgtcgc ctggaaggtg cgggtgcacg cctcgcgccg
     2941 gacggtgtcg gccagttcct ccttgctgcc gaagtggaag gacagggcgc ccttgctcat
     3001 cgccgcggca cggctgacct cgaagagtgt ggtgcgctgg taaccggagc ggtcgaagga
     3061 tctcgctgcg gccatcgtca gtgcgtgccg cgtgagttct gcgcgctctt gcctcatgga
     3121 gcctgttccc ttcgtgcgta cgggtccgcg gggaaacgga ccgtcagggg cagcagctga
     3181 cggggagcac tgccgcccca gacaaacata caaagtgttc tgttttcatg cgctggaagg
     3241 gactacagag ccgcaggaga aggagtggaa gagtgatgcc ccgccaacgg gcttccgcaa
     3301 catgcttcag acgaggccga gttggcggcg gtaccaggca gcggcaccgg gcagcctcgc
     3361 caacgggccg ggccccgggt cgcatcggca cgcgtcccag agcgattccc cgctgtagaa
     3421 gtggtcctgc gcgagcagtt cgagctgccg ctcacggatc cgtccggggt tcgcgcgcat
     3481 cagggagagg cactgctgcc aggtcaactc cccgctcagc ggcggcagca gccccagggc
     3541 ggccagggtc cggagaaggt cgcgcaggcg cacgggctgg ggatggaccg cgtggtggac
     3601 ggctccgccc gccgccggaa gacggagcgc tgcggccgtg acgagccgtg ccaggtcctc
     3661 caccgccacg agtgagaggc gccccgttcc gcccagccac aaccccggta cgcggcgggc
     3721 gagttcggcc agcgccggca cgacccagcg gtcgccttca ccggtcacga gtccggcgcg
     3781 cagtacgagg gcgccctgac gcaacgcgtg cgtctccccg gcgagccggc tccgactggc
     3841 ggcggacccg ggagcgggcc ccaggtcggt gacggacgcg ccgcggtggg gaccccggcc
     3901 gtagacggcc gtcgtcgaca ggtgcacgac ccgccggaca ccggccctcc gcgcctcctc
     3961 catgagcgct gccgtaccca agtcgttgac caggttgcac tgttgctcgt cggagccgac
     4021 gtgggaggcg gcgttcacga gtacgtccgc tccctcgcag acgccggcca gggtctcggg
     4081 ccgggccagg tccgcgtgga cctgctccac accggcgggc agcgcgacgt gcctgcgagt
     4141 gaaggcacgt acggtcaact cctgagccgt gccagggcgt tcgcgggtgc ggggcgctcc
     4201 gggcgctccg tgtgcgccga gccgtgcgag gacggcggag ccgaggaacc ccgtggcacc
     4261 gctcagcacg acgcgcacga tcagcccgcc gcgagcatcg gatgctccgg attgacgacc
     4321 atctccgcgg cgacctgcgg tggggcgatg cccagcagca tgtagtgcca caggtcggcg
     4381 atgcggtcgg gaaggtcggc gcgggcgttg gcgagcttcg agaagtgctg cacgcccgtg
     4441 tagcagccca ccagcagcac ggcgagtcgg tccacttcga cgtggctgcg caactccccg
     4501 cgtttcgcgg ccgcttcgag ctggccgcgg aagacctgtg cccattcgcg gtacggcgcg
     4561 tcgtcgacga agccgatctc ctcctgctcc acggcgagcc gcaccccggc gcggagcagc
     4621 ttgttggtct gcagttcgaa ggcgagcagg agcgtggtgt cgatgagcct ctggagaccg
     4681 tccggccccg gggggagcac cacggcgtcg cgctgggcga gcatcaccgt gcgggcgagg
     4741 tcgagcttcg aggagaagtg gaagtacagg gcaccctggg tgatgccggc gcggtgcatg
     4801 atgtcggcga tcctcgccgc cctgtagccc ccctcgtcga agacctccgc cgccttctcc
     4861 aagatcaact gccgtgtttc gacggcgcgt tgctgcatcg gtttcgttct cgctgccacc
     4921 atccggcctc gccaccaaag ttctatggtt tccggcacag gtcactcact ctggacacca
     4981 tcccacccgg agggccgccg atgaccgtca cccccgcagt gccggacctg ccggaaagca
     5041 cagcctactc gtcgcccgtc ccccgcgagt tcgtgcacaa gtcggcgcat acggaggtgc
     5101 ttctgaccgg ctggcggcgg ctcggcgaga gagagttcga ggtgggcgcc cagtggccgc
     5161 gggaccacgg ctactggcgt acgggccgcg gcggggtgca ggatccgatg ctgctggtgg
     5221 agacgacccg gcaggtgctg ccgctgctgt cccatgcggt ctaccgcgta cctccggggc
     5281 accagttgat ctggagccac caccactgcg cgttcgccgc gcccttgttg cgtgtgacgg
     5341 accggcccgc cgaggtcgtc ctgcgcctgt ccgtgtcccg gccgccgctg cccggcggcc
     5401 ggctgcggag actggcggtg acgatgaccg ccgcatgcga gggacgcacc ctgggcacgg
     5461 cctcgaccgt cttcagcgcc cactcccccg aggtgtacgc gcggctgcgc gagccccgca
     5521 ccgacccggc cgaggcgatg tcggccgcgc tgccgccgcc ggcgccggtg gtccccgcgc
     5581 tcgtggcgag ggagagcgaa cgggaggtgg ttctcgcccc cgccgaggac gcggcgcgcc
     5641 ggcggctgcg cgtcgtcacc tccagtccgt ggctcttcga ccatccggtc gatcacgtac
     5701 ccgggatgct gctgatggag gcggcccgcc agtgcgctca tctcgtgctg cagggcaggc
     5761 ggtcccgtct gctccatctg gtgcgcatgg aggccgagtt caagcgctac gtcgacctgg
     5821 acgaacccgc gtgggtcgac tggtcgcagc cctcctcccg cccggactcc ccctcggagg
     5881 acggcgagga gacgatgacc gtcgcgatca cccagcgagg cgtgcgcgcc ttcaccgccg
     5941 tggtgggctt cgctgccgca gcggtccttc ccggcgccgg gtgacgacgc ctccgggacg
     6001 ggcggaggcg tccgggccca cccgggcgca cagccgcacg ctcgggtatc gtccgtgctt
     6061 tcggtacggc gcgaaagagg aggtgggacc gatcaccgca gcaccagagg ggctgcactc
     6121 ccccgccgtc gccggcccgg gactggcgta ggcggccggg agcccccgtg gcaccactcg
     6181 aaaggcccgc atgacctcgc cgctcccctc tcccctcccc cgttccgctc tcgtcgcccg
     6241 cctccgcgcc gctggctgcg tgttcgccga ggacgaggcg gacctcctga tctccaccgc
     6301 cggttcgccg cacgagctgg ccacgatggc cggccgccgg acagcgggcg agcccctcga
     6361 acacgtcctg ggctgggccg ggttctgcgg gatgcgcacg gcggtcgagg cgggcgtgtt
     6421 cgtccctcgg cggcgcacgg agttcctggt acgtcaggcc gccgaggcgc tccgcgcggt
     6481 cgccggccgg tgccgggaag aggcggccgc ccccgtgctc gtcgacctgt gctgcggttc
     6541 gggcgcggtc ggggcggcgc tcgcctccca ggcggacggg cgcgtcgagc tgcacgcggg
     6601 cgatgtcgat ccggccgccg tacggtgcgc ccggcacaac ctcgccgcct acggcgggca
     6661 cgtccaccac ggcgacctgt acgagccact gcccgccggg ctgcggggac gtgtggacgt
     6721 gctggtggcc aacgtgccct acgtcccggc ggacgaggtc cccctccttc cgccggaagc
     6781 ccgcctccac gaaccccggg tggcgctgga cggcggcccg gacgggctcg acgtgctgcg
     6841 ccgggtcgcc gcgggtgctc ccggctggct ggccccgggc ggttcccttc tggtggagac
     6901 gagcgaacgg caggcgcccc gcgctctgga ggcggtcgcg gactgcgggc tggtggcccg
     6961 ggcggtgacg tgcgaggagt tcgacgcgac cgtcatcacc gcggcacggc cggacgccgg
     7021 ccgggcctga acatgtccga ctcgccttct cggacggtga cttgagggtc gtccgcaccc
     7081 tccgccctac gcggcgacgg cgccgtcccc ggtgaccgtc aaggggcgcg ttccttcatg
     7141 gagtcccgaa cgcggcctcg tcaggcgctg gtcggggccg aaccgggcgc cccgtcctcg
     7201 gcggtgcggg cgttgccgtg cggcccggac ggcgaagggg gcgcggcctc ctgctccttg
     7261 acccgggcgc ggtggcagac gagagtcacc gcgaacgccg agacgagagc cgcgagcacc
     7321 ccgaggttgg cggacgccca cgcccccgag gtgccgccga ggcccagcgg ccccagcagg
     7381 tagccctgcc aggacagcca gccggcggcg gtgttggtca ccaggcccca cccggcggcc
     7441 gtggccgcgc acagcaccgc ggccggaagc caccgcacgt cgccgtagcg gccggagggc
     7501 gagaagaggt ccgcgtcgtc gtagggacgg gagcgcagcg ccacgtcggc cagcatgatg
     7561 ccgcaccagg cggccacggg gacgccgagc gtggtgagga aacccatgaa ggagccgagg
     7621 aagtcctcgg agaagaacac gacgtagacg gttccgagga tcatgagcag cccgtccagc
     7681 gccgcggccg cgtagcgcgg cacccgcacc cccagggcga gcagggcgag ccccgaggag
     7741 tagatgtcca gcaccgcgcc gccgaccagg cccaacacgg ccaccagcgc gaacggcacg
     7801 aggaaccacg tcggcagcag cgtcgtcagg gcgccgacgg ggtcggcggc gatcgcctcg
     7861 ctcagccgcg gggaggaacc agcgagcagc agcccgaaga ccagcaggac gagcggcgcg
     7921 agggacgccc cgaaagtggt ccagcccgcc acggcagcgc ctcgcgtccg gcgcggcagg
     7981 taacgggcgt agtccgcggc ggcgttgacc cagccgagcc cgaagcccgt catcaggaac
     8041 accagcgctc cgacgacctc ctgcgccgag ccggcgggca ggctcccggc cgtctcccag
     8101 cggatctccc cggcgaccag ggcgatgtag acgacggtca gggcagccgt cacgaccgtg
     8161 atcgccgact gcatccgcat gatgaggtcg aagccgacga ctcctgtcag gaccgtcagc
     8221 gccgagacga cgaccagcgc ggcgatcttc gtcgacgtgc cgccgcccca gcccagcctg
     8281 tcgaagacgg tggacgtggc gagcgtcgcg aggatcacca gcacggtctc ccagccgacg
     8341 gtcagcaccc aggagaccgc ggacggcagc ctgttgcccc gcactccgaa cgccgcccgg
     8401 ctgagcacca tcgtcggcgc ggagccgcgc ttgcccgcca cggcgatgaa gccgcacagc
     8461 aggaacgaga agacgatccc cgcgaccccg gcggccagtg cctgccagaa gtcgatcccg
     8521 aaccccagcg cgaacgagcc gtaactcagg cccagcaccg acacgttcga tccgaaccag
     8581 ggccagaaca gctgcgcggg gcggcccttg cgctcggcgt cctcgatgac gttgaggccg
     8641 ttggcctcca cggtgatctt cctcgcggga cggtcaccgt ccgccgccgc gcccggtacg
     8701 tccgttcctg ccccaggcac agtggtcccc cgtatcgccg gatcaaaaag ccagtgggag
     8761 cgttgcgtgc gctgcgcacg gcgtcaagtg ggcgttcgcg ccgggcagtt cggggacggg
     8821 gtgcgggccg ggccggcggc ggggccgggc tcagaagcgc gggctgcacc ccggggtcag
     8881 cggcaggacg ctcagcagcg cggtcagggt cctcgcgtag acgccgtccg gatcggcctg
     8941 gtcgcgcagc ctcgtcaggt gcgtccgggc ctcgggcggc aggtagcagg tcgaccggta
     9001 cggatcgtgg tcgacggcgg cctgtgcctc ctgcggcccg ccgtcggtga caccgcgcgt
     9061 gagccgcagc cagtggggag ccgaccgcgc cagagcggcg tcgacgagcg gctccacgac
     9121 ctcgcccggg tgggagtgcc gcgcggagcc gtgccagacg ctgacgacga ccgcggcaga
     9181 ggaccacgag cgcaaggttg cgggatccgc caggtcacgc cggatgtgat cacgggcccg
     9241 gtagtggcac gcgtcggcgg gggcgagcca gctgccgacg ctgagcccgg cctgacgcag
     9301 cgcgtcggtg acgcagtagg cgacctccgc ggtccccagc acgagcacgt cccgcgtccg
     9361 caccggtgcg ggcgccgcgg cgcgggtggc ggtggcggcg acggggtctg cagccggcat
     9421 ggtctcgtcc ctccgctcca ggtgtcgggg aaaaggcatc tcgggcaatc gggatctcct
     9481 acgaccgtag gcacccgtcg gaggccgcag aagggaggat cggtcgatcc ggggccggta
     9541 caccggtcga tatccgggcc ggcccggggc ggagcgcgct gcgagccgct acttcacgcg
     9601 ggcgacgccc ccgtagcagt tgacctcgtc ggccggcggc ggcacgcccg cgccgggcag
     9661 cggacgccag tccgggaggg agacgacccc ggggtccagc agctccatgc ccgcgaacag
     9721 cgcctcgatc tcctcgtgcg tgcggatgac cagcggactg accgtggagc tgtagtacgc
     9781 ctgcacgctg ccgatgaggt tccaggcgta gtcggcggtc atcgaggtga gggtgacgta
     9841 gctgccgggg gcgacgaacc gccgcacgtc gcggatgagc tttccggcct cgtcgtcgtc
     9901 caggaagtgg acgagggcgt tgacgctgac ggcgacgggc tcgtccaggt cgaggacgtc
     9961 gcgcagcccg ggcgaggaga acagagcgcg cgtgtcgagg acgtcggcct cgatgtacgc
    10021 ggtccggcca cgggcggtgc tgcgcagcag ggccgcggcg tgggcgagga cgatcgggtc
    10081 gtggtcgacg tagacgacgc gggcgccggg cgcggccccc tgcgcgatct cgtggagatt
    10141 cggggaggtg gggatgcccg accccaggtc gaggaactgg cggatgccca tctcggcggt
    10201 gagggtgcgg atggaccgct gcatgaagag ccggttctcc cgtaccgccg tggtggtgtg
    10261 cgggttgatc gcgaggatcc gctcggcggc ctcccggtcg gcgggccagt tgcagttccc
    10321 gccgaggagg tagtcgtaga cccgggcgac atgcggtcgg ccggtgtcca cctcggatac
    10381 gtccaccgtg cgctcccctc ccccggccgg cccgctcaca tgccggtcct ccatcgccgg
    10441 gacatctccc atcatgacgt ctccggtgat gcagagcgcc agcgccgtct cggacgcggg
    10501 ccccgggtac cgcgtcagct cctgctgcgt cgcggtgacc gcccggccca gccgccggta
    10561 cgcggcgcgg ggagtgatgc ccagcgcggc ggctgccgcg tccttgtcca tgtcggcggc
    10621 gacccaggcg gcgagcgtct cccggaggcc cggcgtcagg ccgtccaggc gttcgtccgc
    10681 ccaggcgcgg gcccccgggt ggtccaggac ctggcggagg gtgacagtgc ggtcgagcac
    10741 cggcgggggc ggcagggcgg ccaggcgcac cgcgagatac agcgcgctct gctgccagcg
    10801 ctgccgcggg tccaggcccg tgagcgcggc gaggcggtcc agccgctgtg cgagggtctg
    10861 ccggtgccag ccgacgagcc gcgcggcctc ggtgggcccc caccacagcg tctccccggc
    10921 gaggtgcagc aggtcctccc gctcctggtc gggcaggtcc cgcaacggct gcatcaccag
    10981 cgcc
//
