LOCUS       NZ_LN850107            10375 bp    DNA     linear   CON 13-AUG-2024
DEFINITION  Alloactinosynnema sp. L-07 isolate Alloactinosynnema sp. L-07
            chromosome I.
ACCESSION   NZ_LN850107
VERSION     NZ_LN850107.1
KEYWORDS    RefSeq.
SOURCE      Alloactinosynnema sp. L-07
  ORGANISM  Alloactinosynnema sp. L-07
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1
  AUTHORS   Ramaraj,Thiru.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2015) National Center for Genome Resources (NCGR),
            National Center for Genome Resources (NCGR), 2935 Rodeo Park Drive
            East, Santa Fe, New Mexico, 87505, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900070365.1-RS_2024_08_13
            Annotation Date                   :: 08/13/2024 03:00:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,999
            CDSs (total)                      :: 6,934
            Genes (coding)                    :: 6,831
            CDSs (with protein)               :: 6,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 30 of 103
            Pseudo Genes (incomplete)         :: 83 of 103
            Pseudo Genes (internal stop)      :: 1 of 103
            Pseudo Genes (multiple problems)  :: 11 of 103
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:22:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 7295352
            Orig. end                         :: 7305727
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LN850107.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10375
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="35"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10375
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10375
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5375](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5375
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            290..1132
                     /locus_tag="BN1701_RS37720"
     CDS             290..1132
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /locus_tag="BN1701_RS37720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="WP_054055404.1"
                     /transl_table=11
                     /translation="MSEASTFAASDIRAVTAAELANYRDVLRGAIGVRRVVGAPQASPV
                     YPFVLAMASFDEIVDQLTPEGQPRPGNVHLSQEIRVRRLVAPGERVGIDVEVLAARREA
                     KGVRLAIRCVVVGDDGAVVSELLTGALLVDATTPEPFGDMPAATELTAGPGPVERTAVT
                     ATIPAETVLAYADASGDHNPIHLDEVAAIAAGFPGVIAHGMSVLALITEDVVDRYAGGD
                     AARITGIGCRFSAPILPAEPLEITYVTDGGPVVKFTCKTPRGLAFKGGWVEIDGRRHG"
     gene            1310..2785
                     /locus_tag="BN1701_RS33290"
     CDS             1310..2785
                     /NRPS_PKS="Domain: AMP-binding (14-386). E-value: 6.4e-64.
                     Score: 207.9. Matches aSDomain:
                     nrpspksdomains_BN1701_RS33290_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /locus_tag="BN1701_RS33290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_054055405.1"
                     /sec_met_domain="AMP-binding (E-value: 4.2e-61, bitscore:
                     205.4, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAEPFLQDLVDSFVWHAERAPDAVALVWHDTEIGYGELLDLAHVE
                     RDKLVGHDLATGLPVGILAEKSPEAVALVLACLLARRPFLLPSPALGDDILATLFAQAG
                     CTTVLTTPGQARRMLPPTPVSSPPWPPANTAFMLTTSGSTGQPKIVPLSAQAVARFADW
                     AAPTFGIGPGRTVLNVAPLNFDICLLDVWTTLAAGGRVVMVDPAYAANGRHLAEVLRAN
                     EIHVVQAVPMFFGLLLAAGAEGFDSVEHVMVTGDATPDHVLAGIPLLFPKARLRNIYGC
                     TETNDSFIADVDGLPMAIGDPLPGVRAILVDEGRVITGPGTGELYVSTPFQTEGYLDAS
                     RNVGKFTDHPTGADDLRYFRTGDLVRRGGDGRLMLVGRADFQVKVRGVAVNTAEIEAAL
                     LEHPNVLEAAVAALPDPVAGAVLAAAIHRAPGSGLDSLALREHCVRRLPRAAIPSRMRI
                     TDEPLPKTATGKVDRAAVGRTGTGSSQAERRVS"
     aSDomain        1352..2467
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BN1701_RS33290_AMP-binding.1"
                     /evalue="6.40E-64"
                     /label="BN1701_RS33290_AMP-binding.1"
                     /locus_tag="BN1701_RS33290"
                     /protein_end="386"
                     /protein_start="14"
                     /score="207.9"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="WHAERAPDAVALVWHDTEIGYGELLDLAHVERDKLVGHDLATGLP
                     VGILAEKSPEAVALVLACLLARRPFLLPSPALGDDILATLFAQAGCTTVLTTPGQARRM
                     LPPTPVSSPPWPPANTAFMLTTSGSTGQPKIVPLSAQAVARFADWAAPTFGIGPGRTVL
                     NVAPLNFDICLLDVWTTLAAGGRVVMVDPAYAANGRHLAEVLRANEIHVVQAVPMFFGL
                     LLAAGAEGFDSVEHVMVTGDATPDHVLAGIPLLFPKARLRNIYGCTETNDSFIADVDGL
                     PMAIGDPLPGVRAILVDEGRVITGPGTGELYVSTPFQTEGYLDASRNVGKFTDHPTGAD
                     DLRYFRTGDLVRRGGDGRLMLVGRADFQVKVR"
     CDS_motif       1712..1768
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BN1701_RS33290_0001"
                     /evalue="3.40E-05"
                     /label="NRPS-A_a3"
                     /locus_tag="BN1701_RS33290"
                     /protein_end="153"
                     /protein_start="134"
                     /score="16.6"
                     /tool="antismash"
                     /translation="AFMLTTSGSTGQPKIVPLS"
     CDS_motif       2435..2497
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BN1701_RS33290_0002"
                     /evalue="1.50E-07"
                     /label="NRPS-A_a8"
                     /locus_tag="BN1701_RS33290"
                     /protein_end="396"
                     /protein_start="375"
                     /score="23.8"
                     /tool="antismash"
                     /translation="VGRADFQVKVRGVAVNTAEIE"
     gene            2782..3045
                     /locus_tag="BN1701_RS33295"
     CDS             2782..3045
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PP-binding (18-70). E-value: 1.7e-08.
                     Score: 26.6. Matches aSDomain:
                     nrpspksdomains_BN1701_RS33295_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1147: putative acyl carrier protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680900.1"
                     /locus_tag="BN1701_RS33295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl carrier protein"
                     /protein_id="WP_054055406.1"
                     /sec_met_domain="PP-binding (E-value: 8.1e-06, bitscore:
                     24.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNVSTIKAFIIDEFLPDVAADGLAVDYDLLANGVVDSLGLLKMI
                     AWIEDRFGVPIGDDDLDPDNFRSVAAVDRFIDAARIGPVVGA"
     aSDomain        2836..2991
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BN1701_RS33295_PP-binding.1"
                     /evalue="1.70E-08"
                     /label="BN1701_RS33295_PP-binding.1"
                     /locus_tag="BN1701_RS33295"
                     /protein_end="70"
                     /protein_start="18"
                     /score="26.6"
                     /tool="antismash"
                     /translation="VAADGLAVDYDLLANGVVDSLGLLKMIAWIEDRFGVPIGDDDLDP
                     DNFRSVA"
     gene            3048..4049
                     /locus_tag="BN1701_RS33300"
     CDS             3048..4049
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /locus_tag="BN1701_RS33300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_054055407.1"
                     /transl_table=11
                     /translation="MHEVIAALVQGRGEIDEPAWRSLWDGIESSAVDRAEIAALLASLS
                     TKLPDPESLRALVRSLRVEAAPWPGTVNIVGTGGGPRTFNISTAAAFVAAAMGVPVVKS
                     GSRAYTGSAGSIDLLERLGVGLTKSLDETASTLAKQGVAFTGPFVYPSVLTRMARTLVP
                     LGMRRFGRFLNALGPFIATVPVAAQVTGVSAAVSLADLRELASAQDRRIWLCANDFGAD
                     ELLGFAHNVIHTNDGGPIRLSPGVLARGDGGLADLAEHPGSLVDHFLDVLAGKAHRTAL
                     EAVALNAAALAVASGHTPAWPDAYEAAESALRDGAARSLVDRLRKPTAVLHA"
     misc_feature    3207..3209
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4210..5004
                     /gene="trpA"
                     /locus_tag="BN1701_RS33305"
     CDS             4210..5004
                     /EC_number="4.2.1.20"
                     /GO_function="GO:0004834 - tryptophan synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpA"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /locus_tag="BN1701_RS33305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="WP_054055408.1"
                     /transl_table=11
                     /translation="MGESGLALFLNAGDPPLDVLADLVAMLDESGVDCLELAVPFPGSV
                     TDGLTVRQSANRALAQGVDLDATLDFIAKTRPGLRRLKIALLVDWSHSLKRRSLSTVAD
                     EVAAAGVDGLLVHGLPPRLKADYYLATDAAALPIVTTCYHRKSPPEVLAETAAKASAYV
                     YLVAHYGRSGSAPAAGYADLAGTVARLRETATAPIAVGFGVRTRRDVEAIHGVGADAVI
                     VGSAGVAVVEHALTRDRDVVADFHDFVRSLHPHHPLHTGRTR"
     gene            5001..5375
                     /locus_tag="BN1701_RS33310"
     CDS             5001..5375
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645375.1"
                     /locus_tag="BN1701_RS33310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_054055409.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.6e-31, bitscore:
                     106.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIIRELSGVRTVEWGNGLSRRFLVESDGMGYSLTDTTVRAGTKSL
                     LEYRNHLEACYCIEGSGEVVDMDGNSFPITAGVMYALDKNDAHFLIASPDEDLRLVCVF
                     SPALRGDEVHSLDESGSSHY"
     gene            5466..6605
                     /locus_tag="BN1701_RS33315"
     CDS             5466..6605
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646413.1"
                     /locus_tag="BN1701_RS33315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_054055410.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 3.3e-20, bitscore:
                     71.5, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 5.5e-25, bitscore:
                     86.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 2.9e-32, bitscore:
                     110.6, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEWNADQIDLRAGIAQWCEALSADHVERDEEGTFSWDKWKLVRQ
                     SGILGLPFDERFGGLGLDLVTTMGVLEALGEGNRDGGLSFSVTTSMCSTGVPLEAFGSA
                     ELKQRYLPRICSGELIGAHAITESEGGSDALRMLTTAERDGDEFVLRGSKTFVSNAPIA
                     DVFVVYAKTRPEGGALGVTALLVERDTPGLTVGKSMKKMGLRTSPLSELFFDDCRVPAT
                     QVIGRVGGGFLVLDHVMKREILFSFIINVGEMQHRLDRCLAYAKQRTQFGKPIGHYQSI
                     ANKLVDMRIGVENSRRWLYDTAERLAKGYNVTTEMAIAKLLTSEANLASGLAAVQIFGG
                     HGYMAETGLEKDLRNAVAGTIYSGTTEIQYNRIASMMGL"
     misc_feature    6600..6602
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6602..7318
                     /locus_tag="BN1701_RS33320"
     CDS             6602..7318
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /locus_tag="BN1701_RS33320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="WP_157368356.1"
                     /transl_table=11
                     /translation="MTSPAGRQEILLKTCGATSAADMDVLAAAGADLVGLWHGIPGGPA
                     ELDVDTLVELAAAARATGVLEPVLVTFLSDVDRLGDVLARSGIDWVQLHAYQSPLVVKG
                     LRQKGHSGLKVVKVLHVDDGGCVERRLVPAYERAGTDVFLLDRVGDNGQVGSTGQQLRE
                     SAVADLVPSLTLPFLLAGGISADNRADYPWLATHPGYLGIDVDTAARDHSGRFSGSNVA
                     AIALEWSTARSREVVG"
     gene            7389..8099
                     /locus_tag="BN1701_RS33325"
     CDS             7389..8099
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /locus_tag="BN1701_RS33325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="WP_197672168.1"
                     /transl_table=11
                     /translation="MSVFIEALLEARRPLIMEVKRRDPHGNDLMGGRTVAEVVAHYETA
                     GAACVSVVTGRWFGGTPELLREVVDRTGKPVLQKDFITRRDHIQQARRCGAAAVLLTAG
                     LLTRSSLANLIDAALSLELTPFVEITSEDEITGITSASQCVIAVNNKDIKTGERDTGDL
                     TRSERMLPFLRASGTRAPVSASGIDTPETAARLADLGYAGLLVGTALLRAPSVGDWLDA
                     FDNSRTTEFARKGA"
     gene            8103..9302
                     /gene="metK"
                     /locus_tag="BN1701_RS33330"
     CDS             8103..9302
                     /EC_number="2.5.1.6"
                     /GO_function="GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene="metK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672683.1"
                     /locus_tag="BN1701_RS33330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methionine adenosyltransferase"
                     /protein_id="WP_054055411.1"
                     /transl_table=11
                     /translation="MNSRLFTSESVTEGHPDKICDAISDSILDALLAKDPRSRVAVETM
                     VTTGQVHVAGEVTTEAYADIPGIVRDRVVEIGYDSSAKGFDGRSCGVNIAIGSQSPDIA
                     QGVDTAHEVRVDDSDGEDEIARQGAGDQGLMFGYACSDTPELMPLPIALAHRLALRLTA
                     VRKSGVMPYLRPDGKTQVTIEYAGDQPVRLDTVVVSSQHAEGVSLDSLLAPDVSEYVVA
                     PELEALGLESSGARLLVNPTGRFVVGGPMGDAGLTGRKIIVDTYGGMARHGGGAFSGKD
                     PSKVDRSAAYAMRWVAKNAVAAGLATRIEVQVAYAIGKAAPVGLFVETFGTETVDPTKI
                     QEAVRAVFDLRPGAIIRDLDLLRPIYAPTAAYGHFGRPDLDLPWERTNRVDALRGFLDI
                     "
     misc_feature    9311..9320
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    9390..9399
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            9407..10123
                     /locus_tag="BN1701_RS33335"
     CDS             9407..10123
                     /NRPS_PKS="Domain: Thioesterase (6-218). E-value: 1.6e-35.
                     Score: 115.5. Matches aSDomain:
                     nrpspksdomains_BN1701_RS33335_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004: thioesterase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /locus_tag="BN1701_RS33335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="thioesterase II family protein"
                     /protein_id="WP_054055412.1"
                     /sec_met_domain="Thioesterase (E-value: 6.4e-32, bitscore:
                     110.2, seeds: 16, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 5.1e-10, bitscore:
                     39.0, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPDPIRLVCLAPAGGSSAMFKSWVSGLPAEFTVAAVDLPGRGAR
                     RREPPATSLVDLAGELASTVSGPGRYALFGHSLGGLLAFETGKRIVADGLPRPEFVVLA
                     GSRPPHQSSARLFAPLLDMDDDALIDALAEMGAVNPILRTSPLRALFLPALRSDLALIV
                     GYHPDATPMALDLMAWHADDDPLATPALGLEWSRYTTAAFTHTALRGDHYFPYDRLPVI
                     TELLRAAASRKVREPA"
     aSDomain        9425..10060
                     /ASF="active site serine inconclusive"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BN1701_RS33335_Thioesterase.1"
                     /evalue="1.60E-35"
                     /label="BN1701_RS33335_Thioesterase.1"
                     /locus_tag="BN1701_RS33335"
                     /protein_end="218"
                     /protein_start="6"
                     /score="115.5"
                     /tool="antismash"
                     /translation="RLVCLAPAGGSSAMFKSWVSGLPAEFTVAAVDLPGRGARRREPPA
                     TSLVDLAGELASTVSGPGRYALFGHSLGGLLAFETGKRIVADGLPRPEFVVLAGSRPPH
                     QSSARLFAPLLDMDDDALIDALAEMGAVNPILRTSPLRALFLPALRSDLALIVGYHPDA
                     TPMALDLMAWHADDDPLATPALGLEWSRYTTAAFTHTALRGDHYFPYDR"
     CDS_motif       9611..9664
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BN1701_RS33335_0001"
                     /evalue="1.30E-07"
                     /label="NRPS-te1"
                     /locus_tag="BN1701_RS33335"
                     /protein_end="86"
                     /protein_start="68"
                     /score="24.1"
                     /tool="antismash"
                     /translation="GRYALFGHSLGGLLAFET"
ORIGIN
        1 accgggccga ggccatcgct ctggcgaagg tcttcggcca gaccccaccg gtgaccgcga
       61 ccaagggcgt caccggccac ctgatcggcg ccgccgggac ggtcgagctg atcgcgacga
      121 tcctcgcgac cgccgccgga accgtcccgc cgaccgccaa ccacgaccga atcgagcctg
      181 gtatggacat cgacctggtg cacggccgac cgcgcccggt ggcctccggc cccgcgctgt
      241 cgaactcgtt cggcttcggc ggccacaacg cctgcctggt ggtggccccg tgagcgaagc
      301 atcgacgttc gccgccagcg acatccgcgc ggtcaccgcc gccgaactcg ccaactaccg
      361 cgacgtgctg cgcggcgcca tcggcgtgcg gcgggtcgtc ggggccccgc aggcgtcgcc
      421 ggtctatccg ttcgtgctcg cgatggccag cttcgatgag atcgtcgacc agctcacccc
      481 cgaggggcag ccccgtcccg gcaatgtcca tctgagccag gagatccggg tccgcaggct
      541 ggtcgcgccg ggggagcggg tgggcatcga cgtcgaggtg ctcgccgccc gccgcgaggc
      601 caagggcgtg cgcctggcga tccgctgtgt cgtggtcggc gacgacggcg ccgtcgtgtc
      661 cgagctgctc accggtgccc tgctggtcga cgcgaccacg ccggagccct tcggtgacat
      721 gcccgccgcc accgagctca ccgccggtcc cggccccgtc gaacgcaccg ccgtcaccgc
      781 gacgatcccc gccgagaccg tgctcgccta cgccgacgcc tcgggcgacc acaacccgat
      841 ccacctcgac gaggtcgccg cgatcgccgc gggcttcccc ggcgtgatcg cacacgggat
      901 gagcgtgctc gccctgatca ccgaggacgt ggtcgaccgc tacgcgggcg gcgacgccgc
      961 gcggatcacc gggatcgggt gccgcttctc cgcgccgatc ctgcccgcgg agccgctgga
     1021 gatcacctac gtcaccgacg gcggcccggt cgtcaagttc acctgcaaga ccccgcgcgg
     1081 cctggcgttc aaaggcggct gggtcgagat cgacgggagg cgccatggct gaggtgttcc
     1141 tgcgggacct ggtgggcagt tctcgcgtgg tttggcacgg caccgacggg aggcgtcatg
     1201 gctgagccgt tcctgcagga tctggtcggc ggtttcgggt ggggcttcgg taggcacgtc
     1261 gagcatgtgg cggccggtct ggtctggctg ggcatcgatg ggaggcgtca tggctgagcc
     1321 gttcctgcag gacctcgttg acagcttcgt gtggcacgcc gagcgcgcgc ccgacgcggt
     1381 cgcgctggtc tggcacgaca ccgagatcgg ctacggcgag ctgctcgacc tcgcccacgt
     1441 cgaacgcgac aagctcgtcg ggcacgacct ggccaccggc ctgcccgtcg gcatcctggc
     1501 ggagaagtcg ccggaggccg tggcgctggt gctggcctgt ctgctggccc gccgcccgtt
     1561 cctgctgcca tcgcccgcgc ttggcgacga catcctcgcc accctgttcg cgcaggcggg
     1621 ctgcaccacg gtcctgacca cgccgggcca ggccaggcgg atgctgccgc ccacgccggt
     1681 ctcctccccg ccgtggccgc ccgcgaacac cgcgttcatg ctcaccacgt ccggctccac
     1741 cgggcagccc aagatcgtcc cgctgagcgc gcaggcggtg gcccgcttcg ccgactgggc
     1801 cgcgccgacc ttcggcatcg gccccggccg caccgtgctc aacgtggcgc cgctgaactt
     1861 cgacatctgc ctgctcgacg tgtggacgac gctggccgcg ggcgggcggg tggtcatggt
     1921 cgacccggcc tacgccgcca acggccgcca cctcgccgaa gtactgcgcg ccaacgagat
     1981 ccacgtcgtg caggccgtgc caatgttctt cggactgctg ctggccgcgg gcgcggaggg
     2041 cttcgacagc gtcgagcacg tcatggtcac cggcgacgcc acccccgacc acgtgctcgc
     2101 cgggatcccg ctgctgttcc ccaaggcgcg gctgcgcaac atctacggct gcaccgagac
     2161 caacgacagc ttcatcgccg acgtcgacgg cttgccgatg gcgatcggcg atccgctgcc
     2221 cggggtgcgc gccatcctgg tcgacgaggg ccgcgtgatc accgggccgg gtacaggcga
     2281 gctgtatgtg tccacgccgt tccagaccga gggctacctc gacgcgtccc gcaacgtcgg
     2341 caagttcacc gaccacccga ccggcgccga cgacctgcgc tacttccgca ccggcgacct
     2401 ggtccggcgc ggcggcgacg gcaggctgat gctggtcggc cgcgccgact tccaggtcaa
     2461 ggtgcgcggc gtcgcggtca acaccgccga gatcgaggcg gcgctgctgg agcaccccaa
     2521 cgtgctggag gccgccgtgg ccgccctgcc cgacccggtc gcgggggccg tgctggccgc
     2581 tgccatccac cgggcgccgg gcagcggcct ggacagcctc gcgctgcggg agcactgcgt
     2641 gcggcggctt ccgcgcgccg ccatcccttc acgtatgcgg atcaccgacg agccgctgcc
     2701 caagaccgcc accgggaagg tcgaccgcgc cgcggtgggc cggaccggga ccgggtcgtc
     2761 tcaagccgaa agaagggtgt catgagcaac gtctccacga tcaaggcgtt catcatcgat
     2821 gaattcctcc ccgatgtcgc cgccgacggg ttggccgtcg attacgacct gctggccaac
     2881 ggtgtggtcg acagcctcgg cctgctgaag atgatcgcgt ggatcgagga ccgcttcggc
     2941 gtcccgatcg gcgacgacga ccttgacccg gacaacttcc gcagcgtcgc cgcggtcgac
     3001 cggttcatcg atgccgcgcg catcggcccc gtggtgggtg cctgaccatg cacgaggtga
     3061 tcgcggccct ggtccagggg cgaggtgaga tcgacgagcc cgcgtggcgc tcgctgtggg
     3121 acgggatcga gtcgagcgcc gtcgaccggg ccgagatcgc cgcattgctg gcgtcgctgt
     3181 ccacgaagct gcccgacccg gagtccttac gcgcactggt gaggtcattg cgcgtggaag
     3241 cggcgccgtg gccgggcacg gtgaacatcg tcggcaccgg cggcggaccg cgcacgttca
     3301 acatctccac cgccgcggcc ttcgtggccg cggcgatggg ggtgccggtc gtcaagtccg
     3361 gctcgcgcgc ctacaccggt agcgcgggct cgatcgacct gctggagcgg ctgggcgtcg
     3421 gcctgaccaa gtcgctcgac gagacggcgt cgacgctggc caagcagggc gtcgcgttca
     3481 ccggcccgtt cgtctacccg tcggtgctga cgcggatggc gcgaaccctg gtgccccttg
     3541 gcatgcggcg cttcggccgg ttcctcaacg cgctgggccc gttcatcgcc acggtcccgg
     3601 tcgccgccca ggtgactggc gtgtcggccg cggtctccct ggccgacctg cgcgagctgg
     3661 cctcggcgca ggaccgccgg atctggctgt gcgccaacga cttcggcgcc gacgagctgc
     3721 tcgggttcgc ccacaacgtg atccacacaa acgacggcgg cccgatccgg ctctcgccgg
     3781 gcgtgctggc ccgcggcgac ggcggtctcg ccgatctcgc ggagcacccg ggatcgctgg
     3841 tcgaccactt cctcgacgtg ctcgcgggca aggcccaccg caccgccctc gaagccgtcg
     3901 ccctcaacgc cgcggcgctg gctgtggcca gtggacacac gcccgcgtgg cccgacgcgt
     3961 acgaggcggc cgagtccgcc ctccgcgacg gcgccgcccg ctcgttggtg gaccgcttgc
     4021 gcaagccaac ggcggtgctc catgcctgag cggcccgagt tgtccacagt ggcgccgatc
     4081 catccacagg cacatcccga tcaccccgaa acccgccaga ccccgataga ctggccaagg
     4141 gccgtccccc cgggacaggg tgggggccgc gcgcgtgggg tttcgggagg tttcttcgag
     4201 cagcggccgg tgggcgagtc ggggttggcg ttgttcctca acgccggtga cccgccgctc
     4261 gacgtgttgg ccgatctcgt cgcgatgctg gacgagtccg gtgtggactg tctggagctg
     4321 gctgtcccgt tcccggggtc ggtgactgac ggcctcacgg tgcggcagtc cgcaaaccgg
     4381 gcactggccc aaggtgtcga cctcgacgcc acgctcgact tcatcgccaa gacccggccc
     4441 ggcctgcgca ggctcaagat cgcgctgctg gtcgactgga gtcactccct caagcgccgg
     4501 tcactgtcca cagtggccga cgaggtcgcc gccgccgggg tcgacggcct gctcgtgcac
     4561 ggcctgccgc cgcggctcaa ggccgactac tacctggcca cggatgcggc ggcgctgccc
     4621 atcgtgacca cctgctacca ccggaagtcc cctccggagg tgctggccga gaccgccgcg
     4681 aaggcgtcgg cctacgtcta cctggtggct cactacgggc gcagcggttc cgcgcccgcg
     4741 gcgggctacg ccgatctggc gggcacggtc gcccgcctgc gcgagacggc caccgcgccg
     4801 atcgcggtcg gcttcggcgt tcgcacgcgg cgcgacgtcg aggcgatcca cggggtcggg
     4861 gccgacgcgg tcatcgtcgg gtccgccggg gtcgccgtgg tggagcacgc cctgacgcga
     4921 gaccgggacg tggtcgcgga cttccacgac ttcgtccggt cactccaccc ccaccaccca
     4981 ctgcacacag ggagaacacg atgatcatcc gtgagctttc cggcgtccgc accgtcgagt
     5041 ggggcaacgg cctcagcagg cgattcctcg tcgagtccga cggcatgggg tacagcctga
     5101 ccgacacgac cgtccgcgcg ggcaccaagt cgctgctgga gtaccgcaac caccttgagg
     5161 cgtgctactg catcgagggg tccggcgagg tcgtcgacat ggacggcaac tccttcccga
     5221 tcaccgcggg cgtgatgtac gcgctggaca agaacgacgc gcacttcctg atcgccagcc
     5281 cggacgagga cctgcgcctg gtgtgcgtgt tctccccggc gctgcgcggc gacgaggtgc
     5341 acagcctcga cgagtccggt tcctcgcact actaactcgc acttgttgaa cggtgttgtc
     5401 agcaggttct ggcggctgat caggccgctg tcggcgtgtt gctgatcgaa tagggcggtg
     5461 tcgcggtgat cgagtggaac gccgatcaga tcgacctgcg cgcgggaatc gcgcagtggt
     5521 gcgaggcgct cagcgccgac cacgtggagc gggacgagga aggtactttc tcctgggaca
     5581 aatggaaact ggtgcgccag tcgggcatcc tgggcctgcc gttcgacgaa cggttcggcg
     5641 gattgggcct ggacctggtc accacgatgg gcgtgctgga ggcccttggc gagggcaacc
     5701 gcgacggcgg cctgagcttc tcggtcacca ccagcatgtg cagcaccggt gtcccactgg
     5761 aggctttcgg ctccgctgag ctgaagcagc gctacctgcc gcggatctgt tccggcgagc
     5821 tgatcggcgc ccacgcgatc accgagtccg agggcggctc cgacgccctg cggatgctca
     5881 ccaccgccga gcgcgacggc gacgagttcg tcctgcgcgg cagcaagacc ttcgtcagca
     5941 acgccccgat cgccgacgtg ttcgtcgtct acgccaagac ccgcccagag ggcggtgcgc
     6001 tcggggtcac ggcgctgctg gtcgagcggg acacccccgg cctgaccgtg ggcaagtcca
     6061 tgaagaagat gggcctgcgc acgtcaccgc tcagcgagct gttcttcgac gactgccggg
     6121 tccccgccac ccaggtcatc ggccgggtgg gtggcgggtt cctggtcctc gaccacgtga
     6181 tgaagcggga gatcctgttc tccttcatca tcaatgtcgg cgagatgcag caccggctcg
     6241 accgctgcct ggcctacgcc aagcagcgca cccagttcgg caagccgatc ggccactacc
     6301 agtccatcgc gaacaagctc gtggacatgc gcatcggcgt ggagaactcc cgccgctggc
     6361 tctacgacac cgccgagcgg ttggccaagg gctacaacgt caccaccgag atggccatcg
     6421 ccaagctgct gaccagcgag gccaacctcg cctccggcct ggccgcggtg cagatcttcg
     6481 gcggccacgg ctacatggcc gagaccggac tggagaagga cctccgcaac gccgtcgcgg
     6541 gcaccatcta ctcgggcacg accgagatcc agtacaaccg aatcgcatcg atgatggggt
     6601 tatgaccagc cccgcgggca ggcaggagat cctgctcaag acgtgcggcg cgacttcggc
     6661 cgccgacatg gacgtgctcg ccgccgccgg tgccgacctc gtcgggctct ggcacggcat
     6721 cccgggcggt ccggccgaac tcgacgtcga cacgctcgtg gagttggccg ccgcggcgcg
     6781 ggcgaccggc gtgctggaac cggtcctggt gacgttccta agcgacgtcg accggctcgg
     6841 cgatgtgctc gcccgcagcg ggatcgactg ggtgcagctg cacgcctatc agtcgccgct
     6901 ggtggtcaag ggcctacgcc agaaaggcca cagtggactg aaggtggtca aggtgctgca
     6961 cgtcgacgac ggcggctgcg tcgagcgcag gctggtcccg gcctacgaac gcgcgggcac
     7021 cgacgtgttc ctgctcgacc gggtcggcga caacggccag gtcggcagca ccgggcagca
     7081 actgcgggag tccgcggtgg cagacctggt gcccagtctc accctgcctt tccttctcgc
     7141 gggcgggatc agcgccgaca accgtgccga ctacccgtgg ctggccacac accccggcta
     7201 cctcggcatc gacgtggaca ccgccgcccg tgaccacagt ggacgcttct ccggaagtaa
     7261 tgtggcggct atcgccttgg agtggagcac ggctcgatcg cgagaggtcg tcggatgagc
     7321 gagcttgcga gcgaatcatt gacacagggc gaccgcgagc ggcggcccgg agcctgcgag
     7381 gggccgtcgt gagtgttttc attgaggctt tgctggaagc gcggcgcccg ttgatcatgg
     7441 aggtcaagcg gcgcgacccg cacggcaacg acctgatggg cggtcggacc gtcgccgagg
     7501 tcgtcgccca ctacgagacc gcgggcgccg cgtgcgtctc agtggtgacc ggccgctggt
     7561 tcggcggcac ccccgagttg ctgcgtgagg tcgtcgaccg caccgggaaa ccggtgctgc
     7621 agaaggactt catcacccgc cgcgaccaca tccagcaagc ccgccgctgc ggcgcggccg
     7681 cggtcctgct caccgccggg ctgctgaccc gctccagcct ggccaacctg atcgacgcgg
     7741 ccctgtcgct ggaactgacc ccgttcgtcg agatcacctc cgaggacgag atcaccggca
     7801 tcacgtcggc ctcgcagtgc gtcatcgcgg tcaacaacaa ggacatcaag accggcgagc
     7861 gcgacacggg tgacctgacc aggagcgaac ggatgcttcc gttcctgcgc gcgtcgggaa
     7921 cccgggcgcc ggtcagcgcc agcgggatcg acacccctga gaccgccgcc cggctcgccg
     7981 acctcggcta cgcgggcctg ctggtcggca ccgccctact ccgcgcgccg agcgtggggg
     8041 actggctcga cgcgttcgac aactccagga ccaccgaatt cgcccggaaa ggggcctgat
     8101 ccatgaacag ccgcctgttc acctccgagt cggtcaccga gggacacccg gacaagatct
     8161 gcgacgcgat cagcgactcg atcctcgacg cgctgctcgc caaggacccg cgctcgcgcg
     8221 tcgccgtcga gacgatggtg accaccggcc aggtgcacgt cgcgggcgag gtcaccaccg
     8281 aggcgtacgc cgacatcccc ggcatcgtcc gcgaccgcgt ggtggagatc ggctacgact
     8341 cgtcggccaa gggcttcgac ggccgctcct gcggggtcaa catcgccatc gggtcgcagt
     8401 ccccggacat cgcccagggc gtcgacaccg cccacgaggt gcgcgtcgac gacagcgacg
     8461 gcgaggacga gatcgcccgc cagggcgcag gtgaccaggg cctgatgttc ggctacgcgt
     8521 gcagcgacac ccccgagctg atgccgctgc cgatcgccct ggctcaccgc cttgccctgc
     8581 ggctgaccgc ggtccgcaag tccggcgtga tgccgtacct gcggcccgac ggcaagaccc
     8641 aggtcaccat cgagtacgcc ggagaccagc cggtccggct cgacaccgtc gtcgtgtcgt
     8701 cgcagcacgc cgagggcgtg agcctggaca gcctgctcgc cccggacgtc agcgagtacg
     8761 tcgtggcccc ggaactggag gcgctcggcc tggagagctc gggcgctcgc ctgctggtga
     8821 acccgaccgg ccgcttcgtc gtcggcggcc cgatgggcga cgcgggcctg accggccgca
     8881 agatcatcgt cgacacctac ggcggcatgg cccgccacgg cggcggcgcg ttctcgggca
     8941 aggacccgtc gaaggtcgac cgctcggccg cgtacgcgat gcgctgggtg gcgaagaacg
     9001 cggtggccgc cggtctggcc acgcgcatcg aggtgcaggt cgcctacgcc atcggcaagg
     9061 cggccccggt cggcctgttc gtggagacct tcggcaccga gacggtcgac ccgaccaaga
     9121 tccaggaggc cgtccgcgcg gtgttcgacc tgcgcccggg cgcgatcatc cgtgatctcg
     9181 atctcctgcg cccgatctac gccccgaccg cggcctacgg tcacttcggc aggcccgacc
     9241 tcgacctgcc ctgggagcgc accaaccgcg tcgacgccct gcgcgggttt ctcgacatct
     9301 gatccatgcg gaaggcggcc cggacccatg acacccgacc gggttcgagt tggcgagccg
     9361 cgcgggtttc tcgacatctg atccatgcgg aaggcggccc ggacccatga cacccgaccc
     9421 gatccgactg gtgtgcctcg cccccgcggg cggctcgtcg gcgatgttca agtcatgggt
     9481 ctcgggcctg cccgcggagt tcaccgtcgc ggcggtggac ctccccggcc ggggggcccg
     9541 ccgccgcgag ccacccgcga cgtccctggt ggatctcgcc ggtgagctgg cgtccactgt
     9601 gtccggtccg ggtcggtacg cgctgttcgg ccacagcctc ggcggcctgc tcgccttcga
     9661 gaccggcaag cggatcgtcg cggatggact gccgcgcccg gagttcgtgg tgctggccgg
     9721 ttcccggccg ccgcaccagt cgtcggcccg gctgttcgcg cccctgctgg acatggacga
     9781 cgacgcgctg atcgacgccc tggccgagat gggcgcggtc aacccgatcc tgcgcacctc
     9841 accgcttcga gcgctgtttc tgcccgcgct gcgctccgac ctcgcgctga tcgtgggcta
     9901 ccaccccgac gccaccccga tggcgctgga cctgatggcc tggcacgccg acgacgaccc
     9961 gctggccacc cccgccctcg gcctcgaatg gtcccgctac accacggccg cgttcaccca
    10021 caccgccctg cgcggcgacc actacttccc atacgaccgg ctccccgtca tcaccgaact
    10081 cctccgcgcc gcggcgagtc ggaaggtgcg cgagcccgcc tagatctccc ggcggcgagg
    10141 cctcggttca gaccgactag ccactggtcc ctaggagttc cccaaagcgg aactcacggt
    10201 ctgattcggg gcctcgccgc cgggctcgac gcacaggcga ggacagctgg caactcctcc
    10261 cgtgccggat ggcgcgcacc ggatcgcgcc gactacggtg tcgaccgtgg cggtccgctg
    10321 ttcgtcgcgg gacggacgct gatgtcgagg agaccctgat gccccagtac gcggt
//
