LOCUS       NZ_LN850107            10375 bp    DNA     linear   CON 13-AUG-2024
DEFINITION  Alloactinosynnema sp. L-07 isolate Alloactinosynnema sp. L-07
            chromosome I.
ACCESSION   NZ_LN850107
VERSION     NZ_LN850107.1
KEYWORDS    RefSeq.
SOURCE      Alloactinosynnema sp. L-07
  ORGANISM  Alloactinosynnema sp. L-07
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae.
REFERENCE   1
  AUTHORS   Ramaraj,Thiru.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2015) National Center for Genome Resources (NCGR),
            National Center for Genome Resources (NCGR), 2935 Rodeo Park Drive
            East, Santa Fe, New Mexico, 87505, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900070365.1-RS_2024_08_13
            Annotation Date                   :: 08/13/2024 03:00:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,999
            CDSs (total)                      :: 6,934
            Genes (coding)                    :: 6,831
            CDSs (with protein)               :: 6,831
            Genes (RNA)                       :: 65
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 30 of 103
            Pseudo Genes (incomplete)         :: 83 of 103
            Pseudo Genes (internal stop)      :: 1 of 103
            Pseudo Genes (multiple problems)  :: 11 of 103
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:22:15
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4062650
            Orig. end                         :: 4073025
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LN850107.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10375
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="23"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10375
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10375
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5375](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5375)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(43..825)
                     /locus_tag="BN1701_RS17995"
     CDS             complement(43..825)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /locus_tag="BN1701_RS17995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_054050363.1"
                     /transl_table=11
                     /translation="MLTDSWGWRAAFGILVIDHDPVAEAEFWAMAPAGVAVNAARFASP
                     RRPGTSEYGDQARVVAESPDIARGLDCFGGMRLDAICVCFTTSSFLGGAEFDKAFAEQA
                     SVRAQGIPALTAAQATVAAMDAVGMTAPYVLVPPWFKDEIVAAGREYLTAAGFPPVAMD
                     RLDVGGDLRPWELWDAGAQWEVRPEQVYHQIRRAIPAGADGVVIAGNGLRCVDTIGPLE
                     ADLGLPVVTSNQACLWHCLRTARVDPAAVLGYGRLFTA"
     gene            complement(835..1752)
                     /locus_tag="BN1701_RS18000"
     CDS             complement(835..1752)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680895.1"
                     /locus_tag="BN1701_RS18000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DMT family transporter"
                     /protein_id="WP_054050365.1"
                     /transl_table=11
                     /translation="MSRVNLMLFLLLAAIWGASYTFIKVAVGGLTPAQLVLVRVVLGAA
                     VLYSVLRFTGGRLPKVPAVWGHIAVTAVLGMVAPFLLLAWGEQRTTAAMAGVLIAATPL
                     LTLAASTVALNTERATWRKIVGFGLGFVGVVIVIKPWAEDGELGSLPGQLAVLAAAACY
                     AAQTVYVRKMLSQKGIPPLVSSTTQVIAASILQAAITPFFPWQTPDLRVSVVVSIVLLG
                     VVGTGLAYLIYFRLITDAGAATASSVNYLVPVTAVAISVGLLGEPITWNMIVGTLVVLA
                     ALAFAENRLATKPSTAEPAKVTAE"
     gene            complement(1765..3129)
                     /locus_tag="BN1701_RS18005"
     CDS             complement(1765..3129)
                     /GO_function="GO:0004834 - tryptophan synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680896.1"
                     /locus_tag="BN1701_RS18005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="WP_054050367.1"
                     /sec_met_domain="PALP (E-value: 8.5e-49, bitscore: 165.2,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDPIKYELVESEIPRTWLNPAADIGEVAPIIDPVTREPVGFDGL
                     ARTMAPPLVEQELSSADEFEIPEPVRQHYAKWRCTPLYRARALEQALGTPARIYYKYEG
                     VAPTGSFKPNTAIPQAYYNKLAGKTGLVGETGAGQWGSAVALAAALFGMEAKVFMVKVS
                     FEQKPYRRTLMETYGATCSASPSTETEAGRTILGERPDHPGSLGTAKSEALQFAFANPD
                     YGYTRGSALNHVCAHQSVIGQEAIKQMELADDYPDIVVGAAGGGSNLAGLAFPFLRNQL
                     RGGPELRLIAVEPAACPTLTRGRIAYDHADSQGLTPLFRMHTLGHKFVPPAFHAGGLRA
                     HNIAPLISRAVEDKLMEAVSVRQNDCFEAGVLFARTEGIVPAPESTHAIRGAIDEAIRC
                     REDGTSKAILLGLSGHGDFDLVAYQRYLAGELPDDALSDDALDFGLASIPAPAHV"
     gene            complement(3152..3826)
                     /locus_tag="BN1701_RS18010"
     CDS             complement(3152..3826)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680897.1"
                     /locus_tag="BN1701_RS18010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transglutaminase family protein"
                     /protein_id="WP_054050369.1"
                     /transl_table=11
                     /translation="MIESTAQVTDDDLRETEFLDFTSPVVQAFTEKALRKKGDPTGEIE
                     RAVALYYAVRDGLHYEVYGADAARGAFQASAIIQRGMGFCVHKSIVYAAAVRALGIPSR
                     IVYGDVRNHLSSPRLRELVGGDVFCFHSLVSVFLGGDWVKATPVFNKLLCHVYRIAPLD
                     FDGRADSLYHPYDEEGRQHMEFLRMRGEFADFPYETVVAGISQAHPLLFQSEGSLATEA
                     VR"
     gene            complement(3843..4982)
                     /locus_tag="BN1701_RS18015"
     CDS             complement(3843..4982)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646413.1"
                     /locus_tag="BN1701_RS18015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_054055951.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 1.7e-16, bitscore:
                     59.5, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.8e-25, bitscore:
                     87.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 7.9e-34, bitscore:
                     115.7, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDWNDEQRALRKTIVDLGEKLGAGHIERDQAGEFDQSAWDLVRD
                     TGLLGLPFDPAFGGLGADLLTTMYVLEGLGESCRDGGFNFSVSTHMVSTGVALQRYGSA
                     ALKAKYLPMVCDGSAIGAHAITEPDAGSDMMAMRTTAKADGDVFVLNGSKAFVSNGPIA
                     GLYVVYTLTGKPGSPAAITALLVERDTPGLHVGKPVAKMGLRTSPLCELFFDDCRVPRE
                     NAVGGVGSGFLVLDHVMKWEILASFVINAGEMQHRFQSCVDYAKERAQFGKPIGSFQSV
                     SNKIVDMKVGVETSRKWLYDTAAKLVRRENVTTDIAITKLVVSEANVASAMAAVQIHGG
                     YGYLAEYGMEKELRNAVAGPIYSGTSEVQRQRIAAMVGL"
     gene            complement(5001..5375)
                     /locus_tag="BN1701_RS18020"
     CDS             complement(5001..5375)
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645375.1"
                     /locus_tag="BN1701_RS18020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_054050371.1"
                     /sec_met_domain="ectoine_synt (E-value: 3.6e-27, bitscore:
                     93.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRARSEVKCVDWGNGLSYRFLLGADAMGFTVAHTVVRANTKSR
                     LQYRMHLEACYCIAGAGEVQTPDGRSYRIEPGVLYALDQHDAHFLIADAGADLELISIF
                     NPPLRGDERHNLDADGFSQY"
     gene            complement(5372..5641)
                     /locus_tag="BN1701_RS18025"
     CDS             complement(5372..5641)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1147: putative acyl carrier protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680900.1"
                     /locus_tag="BN1701_RS18025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_054050373.1"
                     /transl_table=11
                     /translation="MSRLAEIKRFVLAEFAPDVGADELADDFDLLDNGVVDSLGLLRLI
                     AWVGLRYDIPVEEVDLTPDNFRSVDAIDDFVGSHIPELIQEGLR"
     gene            complement(5625..7067)
                     /locus_tag="BN1701_RS18030"
     CDS             complement(5625..7067)
                     /NRPS_PKS="Domain: AMP-binding (14-375). E-value: 6.6e-58.
                     Score: 188.1. Matches aSDomain:
                     nrpspksdomains_BN1701_RS18030_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /locus_tag="BN1701_RS18030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_082859917.1"
                     /sec_met_domain="AMP-binding (E-value: 9.3e-59, bitscore:
                     197.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDASAGHLWPGDSAAALSWQGVATGYRDLRSEVARAAELLAELP
                     GDEPVALIPHKSPAGLALVAACTRIERPCLLLPVDLGARSRAALLDQAGCRFLLRADAR
                     TVEEVGAPAAVPDLDGVGLLLTTSGSTGTPKIVPLPRRATAAFIDWAATEFGIGPGTVV
                     FSYAPLNFDLSLLDVWTTLAKGGHVVLAEQDRAVDGKYLLSVFTEAAVEVVQAVPLFFR
                     LLLDAADGRRFPAVRELVTTGEAMPPALAARLPDAFPNARLRNVYGSTETNDSFIHDID
                     PVSGLATPIGTPIAGVRALVVRDGQVLTGAGAGELWTATPFQSGRYLDPALTARSWVEA
                     PAGAGTYFRTGDNVTRDADGVLRLDGRSDFQVKVRGVRTNLLEVEHIVTAHPAVHEAVV
                     LGLPDDVAGHRLHAVVRLCPDATLNSVQLRQHCAARLPRTAIPSTMDIVVDPFPASPNG
                     KVDRAAIQASAREGVREHVAAR"
     CDS_motif       complement(5913..5972)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BN1701_RS18030_0002"
                     /evalue="1.50E-06"
                     /label="NRPS-A_a8"
                     /locus_tag="BN1701_RS18030"
                     /protein_end="385"
                     /protein_start="365"
                     /score="20.7"
                     /tool="antismash"
                     /translation="GRSDFQVKVRGVRTNLLEVE"
     aSDomain        complement(5943..7025)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_BN1701_RS18030_AMP-binding.1"
                     /evalue="6.60E-58"
                     /label="BN1701_RS18030_AMP-binding.1"
                     /locus_tag="BN1701_RS18030"
                     /protein_end="375"
                     /protein_start="14"
                     /score="188.1"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="AAALSWQGVATGYRDLRSEVARAAELLAELPGDEPVALIPHKSPA
                     GLALVAACTRIERPCLLLPVDLGARSRAALLDQAGCRFLLRADARTVEEVGAPAAVPDL
                     DGVGLLLTTSGSTGTPKIVPLPRRATAAFIDWAATEFGIGPGTVVFSYAPLNFDLSLLD
                     VWTTLAKGGHVVLAEQDRAVDGKYLLSVFTEAAVEVVQAVPLFFRLLLDAADGRRFPAV
                     RELVTTGEAMPPALAARLPDAFPNARLRNVYGSTETNDSFIHDIDPVSGLATPIGTPIA
                     GVRALVVRDGQVLTGAGAGELWTATPFQSGRYLDPALTARSWVEAPAGAGTYFRTGDNV
                     TRDADGVLRLDGRSDFQVKVR"
     CDS_motif       complement(6651..6698)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_BN1701_RS18030_0001"
                     /evalue="1.80E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="BN1701_RS18030"
                     /protein_end="139"
                     /protein_start="123"
                     /score="14.5"
                     /tool="antismash"
                     /translation="LLTTSGSTGTPKIVPL"
     misc_feature    complement(7041..7043)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    7069..7083
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.12"
                     /tool="antismash"
     gene            complement(7486..8100)
                     /locus_tag="BN1701_RS18035"
     CDS             complement(7486..8100)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698246.1"
                     /locus_tag="BN1701_RS18035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2567 domain-containing protein"
                     /protein_id="WP_054050377.1"
                     /transl_table=11
                     /translation="MPEPTESEVETPVSEAAVRVETPPPPVFPLWHQPRPKVRIKADLL
                     PAVSIASFIALFGMAIGWLWSRLAPAQLVVVAEGGKLIPLRAESYHRFDDLVLFVLLGL
                     GAGVVTGAGVWMLRERRGPVIMIAAVVGSGLAAWLAMQVGVSWAEARFVVEAAPKIGDV
                     LPVAPRLESRWVVVGWPLGAALAYGFAAAWNGRDDLGRRLG"
     misc_feature    complement(8065..8080)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.99"
                     /tool="antismash"
     gene            complement(8139..8342)
                     /locus_tag="BN1701_RS37370"
     CDS             complement(8139..8342)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684527.1"
                     /locus_tag="BN1701_RS37370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_054055952.1"
                     /transl_table=11
                     /translation="MFCVHCGRPPADGDHTPCQGGRAALEPPRFCGECARRMVVQVTPS
                     GWSARCSRHGETTSANGPVLTD"
     gene            complement(8345..9382)
                     /gene="bioB"
                     /locus_tag="BN1701_RS18045"
     CDS             complement(8345..9382)
                     /EC_number="2.8.1.6"
                     /GO_function="GO:0004076 - biotin synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="bioB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF06968"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684528.1"
                     /locus_tag="BN1701_RS18045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="biotin synthase BioB"
                     /protein_id="WP_054050378.1"
                     /sec_met_domain="PF04055 (E-value: 1.8e-15, bitscore: 56.4,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="PF06968 (E-value: 8.5e-12, bitscore: 43.7,
                     seeds: 824, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATAEHTQDSSIAGDILAVARDQVLERGEGLSEEQVLQVLRLGD
                     DRLDDLLALAHDVRMKWCGPEVEVEGIVSVKTGGCPEDCHFCSQSGRFPTPVRSAWLDI
                     PGLVEAARQTAETGATEFCIVAAVRGPDARLLSQVREGIKAIRESGNDIQIACSLGMLT
                     QEQVDELVAMGVHRYNHNLETAKSHFPNVVTTHSWEERWDTLRMVREAGMEVCSGGIIG
                     MGETYEQRAEFAVQLAELDPHEVPMNFLIPQPGTPYEKYDPIEGKDALRVVAAFRLALP
                     RTMLRFAGGRELTLGDLGAEKGMLGGVNAIIVGNYLTNLGRPAQQDLDMLADLKMPIKA
                     LSDSL"
     gene            complement(9452..10174)
                     /gene="bioD"
                     /locus_tag="BN1701_RS18050"
     CDS             complement(9452..10174)
                     /EC_number="6.3.3.3"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="bioD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153148.1"
                     /locus_tag="BN1701_RS18050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dethiobiotin synthase"
                     /protein_id="WP_054050380.1"
                     /transl_table=11
                     /translation="MSVLVITGTGTGVGKTVVTAGVAALALALRRRVAVLKPAQTGVAA
                     HEPGDLDDVRRLAGAVTTRELRRFPDPLAPDTAARRVGMPPVRPSEVAQAVGELAVTHD
                     LVLVEGAGGLLVRFDPDGGTLADVAWSLAAPTLIVAEAGLGTLNATALTAEALDRRGIT
                     CAGVVVGSWPAEPDLAALLNLDDLPSVAGAPLVGVFPAGAGALDPDEFAQVARRSLAPS
                     LGGEFDVKEFVEQVSSGA"
     misc_feature    10227..10240
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 19.39"
                     /tool="antismash"
ORIGIN
        1 ggtgggcagg cccgcgtact cggtgtccga cttcgccagg gactacgccg tgaacagtcg
       61 cccgtaaccg aggacggccg ccggatccac ccgcgcggtg cgcaggcagt gccacaggca
      121 cgcctggttc gacgtgacca ccggcagtcc cagatcggcc tccaacggcc cgatggtgtc
      181 gacgcaccgc aggccgttgc ccgcgatcac cacgccgtcg gcgcctgctg gaatcgcgcg
      241 gcggatctgg tggtagacct gttccgggcg cacctcccac tgtgcgcccg cgtcccacag
      301 ttcccatggt cgcaggtctc cgccgacgtc caaccggtcc atggcgaccg gcgggaaacc
      361 ggccgctgtc aggtactccc gccctgcggc gactatctcg tccttgaacc aaggtggcac
      421 caggacgtac ggcgccgtca tgcccaccgc gtccatggcc gcgacggtcg cctgggcggc
      481 cgtcagcgcg gggatgccct gggcgcgaac gcttgcctgc tccgcgaagg ctttgtcgaa
      541 ctccgctcca ccgaggaagc tgctggtggt gaagcacacg cagatcgcgt cgagtcgcat
      601 tccgccgaag cagtccaggc cgcgggcgat atccggggac tcggccacca cccgcgcctg
      661 gtcgccgtac tccgaggttc ccggcctgcg cgggctggcg aagcgggccg cgttcaccgc
      721 gaccccggcc ggtgccatgg cccagaactc cgcctccgcc accggatcgt ggtcgatcac
      781 caggatgccg aacgcggcgc gccagcccca gctgtcggtg agcatcggtc ctccctattc
      841 ggcggtgacc ttggcgggtt cggcggtgga aggcttggtg gccagccggt tctcggcgaa
      901 agcgagcgcg gcgaggacga caagcgtgcc gacgatcatg ttccaggtga tcggctcgcc
      961 aagcaggccg acgctgatcg ccacggcggt gaccggcacc aggtagttga ccgaggacgc
     1021 ggtggcggct ccggcgtcgg tgatgagtcg gaagtagatc aggtacgcga ggccggtgcc
     1081 gaccacaccg aggagcacga tgctgaccac gaccgacacg cgcagatccg gggtctgcca
     1141 ggggaagaac ggtgtgatgg ccgcctgcag gatactggcc gcgatcacct gcgtggtgga
     1201 cgagaccagt ggtgggatcc ccttctgcga caacatcttc cgcacataca ccgtctgcgc
     1261 ggcatagcac gccgccgcgg cgagtacggc caactggccg ggcagcgaac ccagttcccc
     1321 gtcctcggcc cacggcttga tcacgatgac aacaccgacg aagccgaggc cgaagcccac
     1381 gatcttgcgc cacgtggccc gttcggtgtt gagcgcgacc gtcgacgcgg ccagggtcag
     1441 cagcggggtc gccgcgatga gcacgcccgc catcgccgcg gtggtgcgct gttcacccca
     1501 cgccagcaac agaaacggcg cgaccatgcc gagaaccgcg gtgacagcga tgtgtcccca
     1561 caccgcgggc accttcggga gcctgccgcc ggtgaaccgc agcaccgagt agagcacagc
     1621 ggcgcccagc accacccgca ccaacaccag ttgagccggg gtcaggccgc cgacggcgac
     1681 cttgatgaac gtgtagctgg cgccccagat cgcggcgagc agcaggaaca gcatcaggtt
     1741 gacacgactc atcttctgcg gtccttaaac gtgggcgggg gcggggatgc tggccagccc
     1801 gaagtcgagg gcgtcgtcgg acagcgcgtc gtcgggcagt tcaccggcaa ggtagcgctg
     1861 gtaggccacg agatcgaagt ccccgtggcc ggacaggccg agcaggatgg ccttgctggt
     1921 gccgtcctcg cggcagcgga tggcctcgtc gatggcgccg cggatcgcgt gcgtcgactc
     1981 gggggcgggc acgatgcctt cggtcctggc gaacagcacg cccgcctcga agcagtcgtt
     2041 ctgccgcacc gagaccgcct ccatcagctt gtcctcgacg gcgcggctga tcagcggggc
     2101 gatgttgtgc gcgcgcaggc cgcccgcgtg gaaggcgggc gggacgaact tgtgccccag
     2161 ggtgtgcatg cggaacagcg gcgtcagacc ctgcgaatcg gcgtggtcgt aggcgatgcg
     2221 gccgcgggtc agcgtcgggc aggcggcggg ctcgacggcg atgagccgca gctccggacc
     2281 gccgcgcaac tggttgcgca ggaaggggaa ggccaggcct gccaggttgc tgccgccgcc
     2341 cgcggcgccg acgacgatgt cggggtagtc gtcggcgagc tccatctgct tgatggcttc
     2401 ctggccgatg accgactggt gggcgcacac gtggttgagc gcgctgcctc tggtgtagcc
     2461 gtagtccggg ttggcgaagg cgaactgcag cgcctccgac ttggcggtgc cgaggctgcc
     2521 ggggtggtcg ggccgttcgc ccaggatcgt gcggcccgcc tcggtctcgg tgctggggct
     2581 ggccgagcag gtcgccccgt aggtctccat cagggtgcgc cggtagggct tctgctcgaa
     2641 gctgaccttg accatgaaga ccttggcttc catgccgaac aaggccgcgg ccagcgccac
     2701 cgcgctgccc cactggcccg cgcccgtctc gccgaccagg ccggtcttgc ccgcgagctt
     2761 gttgtagtac gcctgcggga tcgcggtgtt gggcttgaag ctgccggtgg gcgcgacgcc
     2821 ctcgtacttg tagtagatcc gggcgggcgt gcccagcgcc tgctccagcg cacgggcgcg
     2881 gtagagcggg gtgcagcgcc acttggcgta gtgctggcgg accggctcgg ggatctcgaa
     2941 ctcgtcggcg ctgctcagtt cctgctcgac cagcggcggc gccatcgtcc tggccagccc
     3001 gtcgaagccc accggctcgc gcgtcaccgg gtcgatgatg ggggccacct cgccgatgtc
     3061 ggcggccggg ttgagccagg tgcgggggat ctccgactcg acgagttcgt acttgatcgg
     3121 atcgatcatg tgcttcgtcc ttagtgtgtt actagcgaac ggcttcggtg gcgagcgatc
     3181 cctcggactg gaacagcagc ggatgggcct ggctgatgcc cgcgacgacg gtctcgtagg
     3241 ggaagtcggc gaactcgccg cgcatccgca ggaactccat gtgctgacgg ccctcttcgt
     3301 cgtacgggtg gtagaggctg tccgcgcggc cgtcgaagtc cagcggggcg atccggtaga
     3361 cgtggcagag cagcttgttg aacacgggcg tggccttcac ccagtcgccg ccgaggaaga
     3421 ccgagacgag gctgtggaag cagaagacgt ccccgccgac gagttcacgc agccgcggtg
     3481 aggacaggtg gttgcggacg tcgccgtaga cgatgcggct ggggatgccc agcgcgcgca
     3541 cggccgcggc gtacacgatc gacttgtgca cgcagaaacc catgccgcgc tggatgatcg
     3601 cgctcgcctg gaacgcgccg cgggccgcgt cggccccgta gacctcgtag tgcagaccgt
     3661 cccgcaccgc gtagtacagg gcgacggcgc gctcgatctc gccggtcggg tcgcccttct
     3721 tgcgcagtgc cttctcggtg aacgcctgga ccaccgggct ggtgaagtcc aggaactcgg
     3781 tctcccgcag gtcgtcgtcg gtgacctggg cagtggactc gatcatgctt cctctccgtg
     3841 gttcacaggc cgaccatcgc cgcgatgcgc tggcgttgga cttcggaggt gcccgagtag
     3901 atcgggcccg ccaccgcgtt gcgcagttcc ttctccatcc cgtactcggc caggtagccg
     3961 tagccgccgt ggatctgcac cgcggccatc gccgaggcga cgttcgcctc actcaccacc
     4021 agcttggtga tggcgatgtc ggtggtgacg ttctcccgcc ggacgagctt cgccgcggtg
     4081 tcgtagagcc acttgcggga ggtctcgacg ccgaccttca tgtcgacgat cttgttggac
     4141 acggactgga acgagccgat cggcttaccg aactgcgcac gctccttggc gtagtccaca
     4201 caggactgga agcggtgctg catctcgcct gcgttgatga cgaacgaggc caggatctcc
     4261 catttcatca cgtggtcgag caccaggaag ccggatccga ccccgccgac cgcgttctcg
     4321 cgcgggaccc ggcagtcgtc gaagaacagc tcacacaacg gcgaggtccg taggcccatc
     4381 ttggcgaccg gcttgcccac gtgcaggcca ggggtgtccc gctcgacgag cagggccgtg
     4441 atcgccgcgg gactgcccgg cttcccggtc agcgtgtaga ccacgtacag cccggcgatc
     4501 gggccgttgc tcacgaacgc cttgctgccg ttgaggacga agacatcgcc gtcggccttc
     4561 gccgtggtgc gcatcgccat catgtccgac ccggcgtcgg gctcggtgat ggcgtgcgcg
     4621 ccgatcgccg agccgtcgca caccatcggc aggtacttgg ccttgagcgc ggccgagccg
     4681 taccgctgca gggccacgcc ggtgctgacc atgtgggtcg acaccgagaa gttgaacccg
     4741 ccgtcgcggc agctctcgcc gagtccctcc aggacgtaca tcgtggtcag caggtccgcg
     4801 cccagcccgc cgaaggccgg gtcgaacggc agcccgagca gtccggtgtc gcgcacgagg
     4861 tcccaggcgg actggtcgaa ctcgcccgcc tggtcccgtt cgatgtgacc ggcgcccagc
     4921 ttctcgccga gatcgacgat ggtcttgcgc agcgcgcgct gctcgtcgtt ccagtcgatc
     4981 accacatgct cctcgcccga tcagtactgg gagaacccgt cggcatccag gttgtgccgc
     5041 tcgtcgccgc gcaggggcgg gttgaagatg ctgatcagct cgaggtccgc gcccgcgtcg
     5101 gcgatcagga agtgggcgtc gtgctggtcg agcgcgtaga gcacgcccgg ctcgatccgg
     5161 tacgagcggc cgtccggggt ctgcacctcg cccgcgcccg cgatgcagta gcaggcctcc
     5221 aggtgcattc ggtactgcag gcgggacttg gtgttggccc gcaccaccgt gtgcgcgacc
     5281 gtgaagccca tcgcgtcggc acccagcagg aagcggtagc tcagaccgtt gccccagtcg
     5341 acgcatttca cctcggacct ggcacgcacg atcatctcaa gccctcctgg atcaactcgg
     5401 ggatgtggga accgacgaaa tcgtcgatgg cgtcgaccga ccggaagttg tccggcgtca
     5461 ggtcgacctc ctcgaccggg atgtcgtagc gcaagccaac ccaggcgatg agtcggagca
     5521 gcccgaggct gtcgacgacg ccgttgtcga gcaggtcgaa gtcatcggcc agttcgtcgg
     5581 ccccgacatc gggggcgaac tcggccagca cgaaccgttt tatctcagcg agccgcgaca
     5641 tgttctcgaa ctccttcacg cgccgatgcc tgtattgccg cgcggtcgac cttcccgttg
     5701 ggactggccg ggaacgggtc gaccacgatg tccatagtgg acggaatagc cgtccgggga
     5761 agccgggccg cgcagtgttg tcgcagctgg acgctgttga gcgtggcgtc cggacacagc
     5821 cggaccaccg cgtgcagccg gtggcccgcc acgtcgtctg gcaggccgag cacgaccgcc
     5881 tcgtggaccg cggggtgcgc ggtgacgatg tgttcgacct cgagcaggtt cgtccgcacg
     5941 ccccggacct tgacctggaa gtcggaccgg ccgtcgagcc gcagcacgcc gtcggcgtcg
     6001 cgggtcacgt tgtcgccggt ccggaagtag gttcccgcgc ccgccggggc ctcgacccaa
     6061 ctgcgggcgg tgagcgcggg gtcgaggtag cggccggact ggaacggcgt cgcggtccac
     6121 aactcccccg cgcccgcccc ggtgagcacc tggccatcgc ggacgaccag cgcccgcacc
     6181 ccggcgatcg gtgtgccgat cggggtggcc agcccgctga ccgggtcgat gtcgtggata
     6241 aagctgtcgt tggtctccgt ggacccgtag acgttgcgga ggcgggcgtt ggggaacgcg
     6301 tcgggcagcc gcgcggccag cgcgggcggc atcgcctcgc cggtcgtgac cagttcgcgc
     6361 acggcgggga agcgtcggcc gtcggcggcg tcgagcagca ggcggaagaa cagcgggacg
     6421 gcctggacga cctcgacggc ggcctcagtg aagacgctga gcaggtattt cccgtccacc
     6481 gcacggtcct gttcggccag tacgacgtgt ccgcccttgg ccaatgtggt ccacacgtcg
     6541 agcaggctga ggtcgaagtt cagcggggcg tagctgaaca ccaccgtgcc cggcccgatg
     6601 ccgaactccg tggcggccca gtcgatgaac gccgccgtcg cgcggcgcgg cagggggacg
     6661 atcttgggcg tcccggtcga tccggaggtg gtcagcagca agccgacccc gtcgaggtcc
     6721 ggcaccgcag cgggcgcccc gacctcctcg acggtgcggg cgtccgcgcg cagcaggaat
     6781 cggcatccgg cctggtcgag cagggccgcg cgactccgcg cgccaagatc gaccggcaac
     6841 agcagacagg gccgttcgat acgcgtgcac gcggcgacca aggcgagacc cgcgggcgac
     6901 ttgtgcggaa tgagcgccac cggctcgtcg cccggcagtt cggccagcag ttcggctgcc
     6961 ctggcgacct cggaccgcag gtcgcggtag ccggtggcca ccccttgcca cgaaagcgcc
     7021 gcggcggaat caccgggcca taaatgacct gcggacgcgt cagacatcat tctcccgaca
     7081 attcacggcc gacaacgagt tcggcgaata acgacattgt taatcgacat tgaggaccgc
     7141 gtccggggga gaattcccgg cccgttgcgg cagaacatta gctacgaaat ccgcccacgc
     7201 caaacctcca tcggagtgat cgactgaatc gagacaccgc cgacaagacc accctgcgtc
     7261 gacaattcag accaatccgg acaacccgga ttgatcttta caggtgaacg gaagccctgg
     7321 ccggcctagt aattctgcat gctcgatcac caacggtgac gacactcatt cgggtcggtg
     7381 catacacaga attagccgga taaatgggaa cggcgatgac ggcgcaaacc cattacgcgc
     7441 aatggtccag cggtgactgg tacgagtgaa ctatgtgcgg atttctcagc cgagcctgcg
     7501 gcccagatcg tcgcgaccat tccacgccgc ggcgaatccg taggccaggg cggcgcccag
     7561 cggccagccg accacgaccc atcgcgattc caaacgtggg gcgaccggca gcacgtcgcc
     7621 gatcttcggc gcggcctcca cgacgaagcg ggcttcggcc caggagacgc cgacctgcat
     7681 ggccagccac gcggccaggc ccgagccgac cacggccgcg atcatgatca ccggcccgcg
     7741 ccgctcgcgc agcatccaca cgcccgcgcc ggtgacgacg cccgcgccga ggccgagcag
     7801 cacgaacagg acaagatcgt cgaaccggtg gtagctctcg gcgcgcagcg ggatcagctt
     7861 gccgccctcg gccaccacga cgagctgcgc cggggcgagc cgggaccaca gccagccgat
     7921 cgccatgccg aacagcgcga tgaacgacgc gatgctcacc gcgggcagca gatcggcctt
     7981 gatccggacc ttcggccgcg gctggtgcca cagcgggaac acgggcggcg gcggggtctc
     8041 gacgcggacg gctgcctcgg acacgggggt ctcgacctct gactccgtgg gttcgggcac
     8101 ccgacgacct ccaactcgcg ttgtacttcc ccgcgagact agtcggtcag cacgggcccg
     8161 ttcgcgctgg tcgtctcccc gtgcctgctg cagcgcgccg accacccgct cggcgtcacc
     8221 tgcacgacca tccgcctggc gcactcgccg cagaacctgg gtggctccaa cgcggcccgg
     8281 ccgccctggc acggggtgtg gtccccgtcc gcgggcggcc gtccgcagtg cacacagaac
     8341 atggtcacag cgagtcgctc agcgccttga tgggcatctt gaggtcggcg agcatgtcga
     8401 ggtcctgctg ggcggggcgg cccaggttgg tcaggtagtt gccgacgatg atcgcgttga
     8461 cgccgccgag catgcccttc tccgcgccca ggtcgcccag ggtcagctcc cggccgcccg
     8521 cgaagcgcag catcgtgcgc ggcagggcca gccggaacgc ggcgacgacg cgcagcgcgt
     8581 ccttgccctc gatcgggtcg tacttctcgt acggggtgcc gggctgcgga atgaggaagt
     8641 tcatcgggac ctcgtgcggg tccagctcgg ccagctgcac cgcgaactcg gcgcgctgct
     8701 cgtaggtctc gcccatgccg atgatgccgc cggagcagac ctccatgccc gcctcgcgca
     8761 ccatccgcag ggtgtcccag cgctcttccc acgagtgggt ggtgacgacg ttcgggaagt
     8821 gcgacttggc cgtctcgagg ttgtggttgt agcggtgcac gcccatcgcg accagctcgt
     8881 cgacctgctc ctgggtgagc atgccgagcg agcaggcgat ctggatgtcg ttgcccgact
     8941 ctcggatcgc cttgatgccc tcgcgcacct gcgagagcag gcgggcgtcg ggcccgcgca
     9001 cggcggcgac gatgcagaac tcggtggcac ccgtctcggc ggtctggcgg gcggcctcga
     9061 ccaggccggg gatgtccagc cacgcggagc gcacgggggt cgggaagcgg cccgactgcg
     9121 agcagaagtg acagtcctcg gggcagccgc cggtcttgac gctgacgatg ccctcgacct
     9181 cgacctccgg gccacaccac ttcatccgga cgtcgtgggc cagcgcgagc agatcgtcga
     9241 gccggtcgtc acccaggcgc aggacctgga gcacctgctc ctcggacaac ccttcgccgc
     9301 gctcaagcac ctggtcccgg gcgacggcga ggatgtcgcc tgcgatcgag ctgtcctgcg
     9361 tgtgctcggc ggttgccgtc actggtgcct cctcgtggtt ccggtccggg caagtctgcc
     9421 tcatggcgga cgagcccggc tgcgatgtcg ctcacgcgcc gctggagacc tgctcgacga
     9481 actccttgac gtcgaactcc ccgcccaggg acggagccag cgacctgcgt gcgacctggg
     9541 cgaactcgtc cgggtcgagc gctccggcac ccgcagggaa cacccctacc aggggtgcgc
     9601 ccgcgaccga ggggagatcg tcgaggttga gcagggcggc gagatcaggc tcggcgggcc
     9661 acgacccgac cacgaccccg gcgcaggtga tcccccgccg gtcgagtgcc tcggcggtca
     9721 gcgcggtggc gttgagcgta cccaacccgg cctcggccac gatcagcgtc ggcgcggcca
     9781 gcgaccaggc cacatcggcg agcgtgcccc catcggggtc gaaccggacc agcagcccgc
     9841 ccgcgccctc gaccagcacc aggtcatggg tcaccgcgag ctcaccgacc gcctgcgcga
     9901 cctccgacgg ccgcaccggg ggcatcccca cccgccgcgc ggcggtgtcg ggggccaacg
     9961 ggtcggggaa ccgacgcaac tcgcgggtgg tcactgctcc ggcgagtctg cgcacgtcat
    10021 cgaggtcgcc cggctcatgc gcggcgacac ctgtctgtgc gggcttgagc acggccaccc
    10081 gccgccggag tgccaacgcg agcgcggcga ccccagccgt gacgacggtc ttgccgacgc
    10141 ccgtgccggt cccggtgatc acaagaacac tcacgtggtg caggttagac ctggcaattg
    10201 gttatggccg ccacacccca acactctgga tatggtcgaa ccgtgagcaa cgacgtcctc
    10261 atctacagcg ccgactggtg cggcgactgc cgccgagcca agtcctggct gcgggccaac
    10321 accgtcccct tcaccgagat cgacgtcgag catgacgaca cggcccggga cctgg
//
